slif cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	slif
Entrez Gene ID	40510
Full Name	slimfast
Synonyms	CG11128,Dmel\CG11128,Slif,slifA
General protein information	Preferred Names slimfast Names CG11128 gene product from transcript CG11128-RA CG11128-PA CG11128-PB CG11128-PC slif-PA slif-PB slif-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 80B2-80B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_141171.3	NP_649428.1	slimfast, isoform C
NM_168978.2	NP_730765.1	slimfast, isoform B
NM_168977.2	NP_730764.1	slimfast, isoform A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SLC7A1	NP_001129264.1
Rattus norvegicus (Norway rat)	Slc7a1	NP_037243.1
Pan troglodytes (chimpanzee)	SLC7A1	XP_001139004.1
Danio rerio (zebrafish)	slc7a1	XP_005155334.1
Homo sapiens (human)	SLC7A1	NP_003036.1
Macaca mulatta (Rhesus monkey)	SLC7A1	XP_002800751.1
Gallus gallus (chicken)	SLC7A1	NP_001138962.1
Drosophila melanogaster (fruit fly)	slif	NP_649428.1
Xenopus tropicalis (tropical clawed frog)	slc7a1	XP_002934074.1
Drosophila melanogaster (fruit fly)	CG5535	NP_730312.1
Canis lupus familiaris (dog)	SLC7A1	XP_005635552.1
Mus musculus (house mouse)	Slc7a1	NP_031539.3
Arabidopsis thaliana (thale cress)	CAT4	NP_187022.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following slif gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slif cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19311	NM_141171.3 Latest version!	Drosophila melanogaster slimfast (slif), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa19311	NM_168978.2 Latest version!	Drosophila melanogaster slimfast (slif), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa19311	NM_168977.2 Latest version!	Drosophila melanogaster slimfast (slif), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slif ( NM_141171.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649428.1 Drosophila melanogaster slimfast (slif), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa19311
Clone ID Related Accession (Same CDS sequence)	NM_141171.3 , NM_168978.2 , NM_168977.2
Accession Version	NM_141171.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	slimfast, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_141171.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCAATC TCTGGAAGGC ATTGACGCGT CGCAAGACGG AGGACGTGAA CGAAGGCGAG 
TCCCAGTTGG CGCGAGTGCT GAACCTTTTC GATCTGACGG CTTTGGGAGT GGGCAGTACT 
TTGGGACTGG GAGTCTATGT GCTGGCTGGT CAGGTGGCCT TCAATATTGC CGGGCCAGCG 
GTGACCATTT CCTTCCTGAT TGCGGCCATC GCGTCTGCCT TCGCGGGGAT CTGCTATGCG 
GAGTTCGCCG CGCGAGTGCC CAAGGCGGGA AGCGCCTACG TATACAGCTA CGTGACCATT 
GGAGAGTTCG TCGCCTTCAC CATAGGATGG AATCTCATTC TGGAGTATGT GATTGGCACA 
GCCTCAGTTG CGCGCGGCCT GAGCGGATAT TTCGACTCTC TGATCAACAA CAACATGTCG 
AAGGCATTGA ATGAGTCGAT GCACATCGAC GTCGACTTTC TGGGTGACTA CCCCGATTTT 
TTGTCCTTCG GCATGGTGCT GCTCCTGGCG GCCATCCTCG CCTTTGGCGC CAAAGAGTCA 
AGCTTCCTGA ACAACATCTT CACCACAGTC AATCTGGTTA CGATCGCTAT CGTCCTGGTG 
GCTGGCGCCA TGAACGCCAA CGTCGACAAT TGGCGCATCC CGAAGAAAGA TGTTCCCGAG 
GGCTTTGGCA CGGGAGGCTT CATGCCGTTC GGCATTGCTG GAGTGATGGC CGGAGCTGCC 
AAATGCTTCT ACGGCTTCGT GGGGTTCGAC TGCATCGCCA CCACGGGCGA GGAGGCCATC 
AACCCAAAGC GCAACATTCC GCTCTCTATC GTGGTGTCGC TGATCATAAT CTTCCTATCC 
TACTTTGGAG TGTCCACGGT GCTGACTATG ATGCTGCCCT ACTACAAACA GGACAAGGAT 
GCACCTTTTC CTCACGCTTT CGATTCCGTC GAGTGGTACA CCATCAAGTG GATTGTGACC 
ATAGGTGCTG TGTTTGCGCT CTGCACTAGT TTGTTGGGCG CCATGTTCCC ACTGCCTCGC 
ATCCTCTACG CCATGGGCAA GGACGGCATA TTGTTCAAGA GGCTGTCCAC AGTGAACAGC 
TACACGAAGA CCCCGCTCCT GGCCACCATT GTGTCTGGAA TCTTTGCGTC CATAATGGCC 
ATGCTCTTTA ACCTGGACCA GCTTGTGGAC ATGATGTCCA TCGGAACCCT GTTAGCATAC 
ACCATCGTGG CAATTTGCGT GCTAGTGCTG CGCTACCAGG ACGAGGAGAT GACCAAGCTG 
GTGTCTGTCA AGGCACCGAA TGTTTTCCGG CAATTCTTCA ACGGCAACTC CTTCCGGGAG 
CCCAATTCTA TGACCTCGTC CATCACCAAA GTCGGCATTG TGGTGTTCGC CATCTTTTGC 
CTGGTCTGGT GCTCCCTCCA GAAAGTCTTC GACCTGGACA GCACCGGCGG AATTGTGGCG 
CTCAGCTTGG TGGGCGCTGT GCTAATCCTC ATCTGCGTTG TGATCGGCAT GCAGCCGGTG 
TCGACCATTG AGCTCACGTT CAAGGTGCCG CTAGTGCCGT TCGTGCCGTG CCTCAGCGTC 
TTTGCGAATT TGTACCTGAT GTTTCAGCTG GACCTGAACA CATGGATCCG CTTCCTCATC 
TGGATCGTGA TCGGCTACGT GATTTACTTC TGTTACGGCA TGCGGAACTC CACGCAGATA 
AGTAGGAGCC GCAGTCACGC CGAGGTGGCG GCAAGCGCTC TGCAGAACCA GGGACAGCAC 
GTGAATCCCG GCTTCGAGCC GGACTACAAG GTCGAAAATG GGGGTCGACC CTACGAGTTC 
TCCGAGAAGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649428.1
CDS	266..2080
Translation	MTNLWKALTRRKTEDVNEGESQLARVLNLFDLTALGVGSTLGLGVYVLAGQVAFNIAGPAVTISFLIAAIASAFAGICYAEFAARVPKAGSAYVYSYVTIGEFVAFTIGWNLILEYVIGTASVARGLSGYFDSLINNNMSKALNESMHIDVDFLGDYPDFLSFGMVLLLAAILAFGAKESSFLNNIFTTVNLVTIAIVLVAGAMNANVDNWRIPKKDVPEGFGTGGFMPFGIAGVMAGAAKCFYGFVGFDCIATTGEEAINPKRNIPLSIVVSLIIIFLSYFGVSTVLTMMLPYYKQDKDAPFPHAFDSVEWYTIKWIVTIGAVFALCTSLLGAMFPLPRILYAMGKDGILFKRLSTVNSYTKTPLLATIVSGIFASIMAMLFNLDQLVDMMSIGTLLAYTIVAICVLVLRYQDEEMTKLVSVKAPNVFRQFFNGNSFREPNSMTSSITKVGIVVFAIFCLVWCSLQKVFDLDSTGGIVALSLVGAVLILICVVIGMQPVSTIELTFKVPLVPFVPCLSVFANLYLMFQLDLNTWIRFLIWIVIGYVIYFCYGMRNSTQISRSRSHAEVAASALQNQGQHVNPGFEPDYKVENGGRPYEFSEKL

Target ORF information:

RefSeq Version	NM_141171.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slimfast (slif), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141171.3

ORF Insert Sequence:

ATGACCAATC TCTGGAAGGC ATTGACGCGT CGCAAGACGG AGGACGTGAA CGAAGGCGAG 
TCCCAGTTGG CGCGAGTGCT GAACCTTTTC GATCTGACGG CTTTGGGAGT GGGCAGTACT 
TTGGGACTGG GAGTCTATGT GCTGGCTGGT CAGGTGGCCT TCAATATTGC CGGGCCAGCG 
GTGACCATTT CCTTCCTGAT TGCGGCCATC GCGTCTGCCT TCGCGGGGAT CTGCTATGCG 
GAGTTCGCCG CGCGAGTGCC CAAGGCGGGA AGCGCCTACG TATACAGCTA CGTGACCATT 
GGAGAGTTCG TCGCCTTCAC CATAGGATGG AATCTCATTC TGGAGTATGT GATTGGCACA 
GCCTCAGTTG CGCGCGGCCT GAGCGGATAT TTCGACTCTC TGATCAACAA CAACATGTCG 
AAGGCATTGA ATGAGTCGAT GCACATCGAC GTCGACTTTC TGGGTGACTA CCCCGATTTT 
TTGTCCTTCG GCATGGTGCT GCTCCTGGCG GCCATCCTCG CCTTTGGCGC CAAAGAGTCA 
AGCTTCCTGA ACAACATCTT CACCACAGTC AATCTGGTTA CGATCGCTAT CGTCCTGGTG 
GCTGGCGCCA TGAACGCCAA CGTCGACAAT TGGCGCATCC CGAAGAAAGA TGTTCCCGAG 
GGCTTTGGCA CGGGAGGCTT CATGCCGTTC GGCATTGCTG GAGTGATGGC CGGAGCTGCC 
AAATGCTTCT ACGGCTTCGT GGGGTTCGAC TGCATCGCCA CCACGGGCGA GGAGGCCATC 
AACCCAAAGC GCAACATTCC GCTCTCTATC GTGGTGTCGC TGATCATAAT CTTCCTATCC 
TACTTTGGAG TGTCCACGGT GCTGACTATG ATGCTGCCCT ACTACAAACA GGACAAGGAT 
GCACCTTTTC CTCACGCTTT CGATTCCGTC GAGTGGTACA CCATCAAGTG GATTGTGACC 
ATAGGTGCTG TGTTTGCGCT CTGCACTAGT TTGTTGGGCG CCATGTTCCC ACTGCCTCGC 
ATCCTCTACG CCATGGGCAA GGACGGCATA TTGTTCAAGA GGCTGTCCAC AGTGAACAGC 
TACACGAAGA CCCCGCTCCT GGCCACCATT GTGTCTGGAA TCTTTGCGTC CATAATGGCC 
ATGCTCTTTA ACCTGGACCA GCTTGTGGAC ATGATGTCCA TCGGAACCCT GTTAGCATAC 
ACCATCGTGG CAATTTGCGT GCTAGTGCTG CGCTACCAGG ACGAGGAGAT GACCAAGCTG 
GTGTCTGTCA AGGCACCGAA TGTTTTCCGG CAATTCTTCA ACGGCAACTC CTTCCGGGAG 
CCCAATTCTA TGACCTCGTC CATCACCAAA GTCGGCATTG TGGTGTTCGC CATCTTTTGC 
CTGGTCTGGT GCTCCCTCCA GAAAGTCTTC GACCTGGACA GCACCGGCGG AATTGTGGCG 
CTCAGCTTGG TGGGCGCTGT GCTAATCCTC ATCTGCGTTG TGATCGGCAT GCAGCCGGTG 
TCGACCATTG AGCTCACGTT CAAGGTGCCG CTAGTGCCGT TCGTGCCGTG CCTCAGCGTC 
TTTGCGAATT TGTACCTGAT GTTTCAGCTG GACCTGAACA CATGGATCCG CTTCCTCATC 
TGGATCGTGA TCGGCTACGT GATTTACTTC TGTTACGGCA TGCGGAACTC CACGCAGATA 
AGTAGGAGCC GCAGTCACGC CGAGGTGGCG GCAAGCGCTC TGCAGAACCA GGGACAGCAC 
GTGAATCCCG GCTTCGAGCC GGACTACAAG GTCGAAAATG GGGGTCGACC CTACGAGTTC 
TCCGAGAAGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slif ( NM_168978.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730765.1 Drosophila melanogaster slimfast (slif), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19311
Clone ID Related Accession (Same CDS sequence)	NM_141171.3 , NM_168978.2 , NM_168977.2
Accession Version	NM_168978.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	slimfast, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168978.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCAATC TCTGGAAGGC ATTGACGCGT CGCAAGACGG AGGACGTGAA CGAAGGCGAG 
TCCCAGTTGG CGCGAGTGCT GAACCTTTTC GATCTGACGG CTTTGGGAGT GGGCAGTACT 
TTGGGACTGG GAGTCTATGT GCTGGCTGGT CAGGTGGCCT TCAATATTGC CGGGCCAGCG 
GTGACCATTT CCTTCCTGAT TGCGGCCATC GCGTCTGCCT TCGCGGGGAT CTGCTATGCG 
GAGTTCGCCG CGCGAGTGCC CAAGGCGGGA AGCGCCTACG TATACAGCTA CGTGACCATT 
GGAGAGTTCG TCGCCTTCAC CATAGGATGG AATCTCATTC TGGAGTATGT GATTGGCACA 
GCCTCAGTTG CGCGCGGCCT GAGCGGATAT TTCGACTCTC TGATCAACAA CAACATGTCG 
AAGGCATTGA ATGAGTCGAT GCACATCGAC GTCGACTTTC TGGGTGACTA CCCCGATTTT 
TTGTCCTTCG GCATGGTGCT GCTCCTGGCG GCCATCCTCG CCTTTGGCGC CAAAGAGTCA 
AGCTTCCTGA ACAACATCTT CACCACAGTC AATCTGGTTA CGATCGCTAT CGTCCTGGTG 
GCTGGCGCCA TGAACGCCAA CGTCGACAAT TGGCGCATCC CGAAGAAAGA TGTTCCCGAG 
GGCTTTGGCA CGGGAGGCTT CATGCCGTTC GGCATTGCTG GAGTGATGGC CGGAGCTGCC 
AAATGCTTCT ACGGCTTCGT GGGGTTCGAC TGCATCGCCA CCACGGGCGA GGAGGCCATC 
AACCCAAAGC GCAACATTCC GCTCTCTATC GTGGTGTCGC TGATCATAAT CTTCCTATCC 
TACTTTGGAG TGTCCACGGT GCTGACTATG ATGCTGCCCT ACTACAAACA GGACAAGGAT 
GCACCTTTTC CTCACGCTTT CGATTCCGTC GAGTGGTACA CCATCAAGTG GATTGTGACC 
ATAGGTGCTG TGTTTGCGCT CTGCACTAGT TTGTTGGGCG CCATGTTCCC ACTGCCTCGC 
ATCCTCTACG CCATGGGCAA GGACGGCATA TTGTTCAAGA GGCTGTCCAC AGTGAACAGC 
TACACGAAGA CCCCGCTCCT GGCCACCATT GTGTCTGGAA TCTTTGCGTC CATAATGGCC 
ATGCTCTTTA ACCTGGACCA GCTTGTGGAC ATGATGTCCA TCGGAACCCT GTTAGCATAC 
ACCATCGTGG CAATTTGCGT GCTAGTGCTG CGCTACCAGG ACGAGGAGAT GACCAAGCTG 
GTGTCTGTCA AGGCACCGAA TGTTTTCCGG CAATTCTTCA ACGGCAACTC CTTCCGGGAG 
CCCAATTCTA TGACCTCGTC CATCACCAAA GTCGGCATTG TGGTGTTCGC CATCTTTTGC 
CTGGTCTGGT GCTCCCTCCA GAAAGTCTTC GACCTGGACA GCACCGGCGG AATTGTGGCG 
CTCAGCTTGG TGGGCGCTGT GCTAATCCTC ATCTGCGTTG TGATCGGCAT GCAGCCGGTG 
TCGACCATTG AGCTCACGTT CAAGGTGCCG CTAGTGCCGT TCGTGCCGTG CCTCAGCGTC 
TTTGCGAATT TGTACCTGAT GTTTCAGCTG GACCTGAACA CATGGATCCG CTTCCTCATC 
TGGATCGTGA TCGGCTACGT GATTTACTTC TGTTACGGCA TGCGGAACTC CACGCAGATA 
AGTAGGAGCC GCAGTCACGC CGAGGTGGCG GCAAGCGCTC TGCAGAACCA GGGACAGCAC 
GTGAATCCCG GCTTCGAGCC GGACTACAAG GTCGAAAATG GGGGTCGACC CTACGAGTTC 
TCCGAGAAGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730765.1
CDS	136..1950
Translation	MTNLWKALTRRKTEDVNEGESQLARVLNLFDLTALGVGSTLGLGVYVLAGQVAFNIAGPAVTISFLIAAIASAFAGICYAEFAARVPKAGSAYVYSYVTIGEFVAFTIGWNLILEYVIGTASVARGLSGYFDSLINNNMSKALNESMHIDVDFLGDYPDFLSFGMVLLLAAILAFGAKESSFLNNIFTTVNLVTIAIVLVAGAMNANVDNWRIPKKDVPEGFGTGGFMPFGIAGVMAGAAKCFYGFVGFDCIATTGEEAINPKRNIPLSIVVSLIIIFLSYFGVSTVLTMMLPYYKQDKDAPFPHAFDSVEWYTIKWIVTIGAVFALCTSLLGAMFPLPRILYAMGKDGILFKRLSTVNSYTKTPLLATIVSGIFASIMAMLFNLDQLVDMMSIGTLLAYTIVAICVLVLRYQDEEMTKLVSVKAPNVFRQFFNGNSFREPNSMTSSITKVGIVVFAIFCLVWCSLQKVFDLDSTGGIVALSLVGAVLILICVVIGMQPVSTIELTFKVPLVPFVPCLSVFANLYLMFQLDLNTWIRFLIWIVIGYVIYFCYGMRNSTQISRSRSHAEVAASALQNQGQHVNPGFEPDYKVENGGRPYEFSEKL

Target ORF information:

RefSeq Version	NM_168978.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slimfast (slif), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168978.2

ORF Insert Sequence:

ATGACCAATC TCTGGAAGGC ATTGACGCGT CGCAAGACGG AGGACGTGAA CGAAGGCGAG 
TCCCAGTTGG CGCGAGTGCT GAACCTTTTC GATCTGACGG CTTTGGGAGT GGGCAGTACT 
TTGGGACTGG GAGTCTATGT GCTGGCTGGT CAGGTGGCCT TCAATATTGC CGGGCCAGCG 
GTGACCATTT CCTTCCTGAT TGCGGCCATC GCGTCTGCCT TCGCGGGGAT CTGCTATGCG 
GAGTTCGCCG CGCGAGTGCC CAAGGCGGGA AGCGCCTACG TATACAGCTA CGTGACCATT 
GGAGAGTTCG TCGCCTTCAC CATAGGATGG AATCTCATTC TGGAGTATGT GATTGGCACA 
GCCTCAGTTG CGCGCGGCCT GAGCGGATAT TTCGACTCTC TGATCAACAA CAACATGTCG 
AAGGCATTGA ATGAGTCGAT GCACATCGAC GTCGACTTTC TGGGTGACTA CCCCGATTTT 
TTGTCCTTCG GCATGGTGCT GCTCCTGGCG GCCATCCTCG CCTTTGGCGC CAAAGAGTCA 
AGCTTCCTGA ACAACATCTT CACCACAGTC AATCTGGTTA CGATCGCTAT CGTCCTGGTG 
GCTGGCGCCA TGAACGCCAA CGTCGACAAT TGGCGCATCC CGAAGAAAGA TGTTCCCGAG 
GGCTTTGGCA CGGGAGGCTT CATGCCGTTC GGCATTGCTG GAGTGATGGC CGGAGCTGCC 
AAATGCTTCT ACGGCTTCGT GGGGTTCGAC TGCATCGCCA CCACGGGCGA GGAGGCCATC 
AACCCAAAGC GCAACATTCC GCTCTCTATC GTGGTGTCGC TGATCATAAT CTTCCTATCC 
TACTTTGGAG TGTCCACGGT GCTGACTATG ATGCTGCCCT ACTACAAACA GGACAAGGAT 
GCACCTTTTC CTCACGCTTT CGATTCCGTC GAGTGGTACA CCATCAAGTG GATTGTGACC 
ATAGGTGCTG TGTTTGCGCT CTGCACTAGT TTGTTGGGCG CCATGTTCCC ACTGCCTCGC 
ATCCTCTACG CCATGGGCAA GGACGGCATA TTGTTCAAGA GGCTGTCCAC AGTGAACAGC 
TACACGAAGA CCCCGCTCCT GGCCACCATT GTGTCTGGAA TCTTTGCGTC CATAATGGCC 
ATGCTCTTTA ACCTGGACCA GCTTGTGGAC ATGATGTCCA TCGGAACCCT GTTAGCATAC 
ACCATCGTGG CAATTTGCGT GCTAGTGCTG CGCTACCAGG ACGAGGAGAT GACCAAGCTG 
GTGTCTGTCA AGGCACCGAA TGTTTTCCGG CAATTCTTCA ACGGCAACTC CTTCCGGGAG 
CCCAATTCTA TGACCTCGTC CATCACCAAA GTCGGCATTG TGGTGTTCGC CATCTTTTGC 
CTGGTCTGGT GCTCCCTCCA GAAAGTCTTC GACCTGGACA GCACCGGCGG AATTGTGGCG 
CTCAGCTTGG TGGGCGCTGT GCTAATCCTC ATCTGCGTTG TGATCGGCAT GCAGCCGGTG 
TCGACCATTG AGCTCACGTT CAAGGTGCCG CTAGTGCCGT TCGTGCCGTG CCTCAGCGTC 
TTTGCGAATT TGTACCTGAT GTTTCAGCTG GACCTGAACA CATGGATCCG CTTCCTCATC 
TGGATCGTGA TCGGCTACGT GATTTACTTC TGTTACGGCA TGCGGAACTC CACGCAGATA 
AGTAGGAGCC GCAGTCACGC CGAGGTGGCG GCAAGCGCTC TGCAGAACCA GGGACAGCAC 
GTGAATCCCG GCTTCGAGCC GGACTACAAG GTCGAAAATG GGGGTCGACC CTACGAGTTC 
TCCGAGAAGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slif ( NM_168977.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730764.1 Drosophila melanogaster slimfast (slif), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa19311
Clone ID Related Accession (Same CDS sequence)	NM_141171.3 , NM_168978.2 , NM_168977.2
Accession Version	NM_168977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	slimfast, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168977.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCAATC TCTGGAAGGC ATTGACGCGT CGCAAGACGG AGGACGTGAA CGAAGGCGAG 
TCCCAGTTGG CGCGAGTGCT GAACCTTTTC GATCTGACGG CTTTGGGAGT GGGCAGTACT 
TTGGGACTGG GAGTCTATGT GCTGGCTGGT CAGGTGGCCT TCAATATTGC CGGGCCAGCG 
GTGACCATTT CCTTCCTGAT TGCGGCCATC GCGTCTGCCT TCGCGGGGAT CTGCTATGCG 
GAGTTCGCCG CGCGAGTGCC CAAGGCGGGA AGCGCCTACG TATACAGCTA CGTGACCATT 
GGAGAGTTCG TCGCCTTCAC CATAGGATGG AATCTCATTC TGGAGTATGT GATTGGCACA 
GCCTCAGTTG CGCGCGGCCT GAGCGGATAT TTCGACTCTC TGATCAACAA CAACATGTCG 
AAGGCATTGA ATGAGTCGAT GCACATCGAC GTCGACTTTC TGGGTGACTA CCCCGATTTT 
TTGTCCTTCG GCATGGTGCT GCTCCTGGCG GCCATCCTCG CCTTTGGCGC CAAAGAGTCA 
AGCTTCCTGA ACAACATCTT CACCACAGTC AATCTGGTTA CGATCGCTAT CGTCCTGGTG 
GCTGGCGCCA TGAACGCCAA CGTCGACAAT TGGCGCATCC CGAAGAAAGA TGTTCCCGAG 
GGCTTTGGCA CGGGAGGCTT CATGCCGTTC GGCATTGCTG GAGTGATGGC CGGAGCTGCC 
AAATGCTTCT ACGGCTTCGT GGGGTTCGAC TGCATCGCCA CCACGGGCGA GGAGGCCATC 
AACCCAAAGC GCAACATTCC GCTCTCTATC GTGGTGTCGC TGATCATAAT CTTCCTATCC 
TACTTTGGAG TGTCCACGGT GCTGACTATG ATGCTGCCCT ACTACAAACA GGACAAGGAT 
GCACCTTTTC CTCACGCTTT CGATTCCGTC GAGTGGTACA CCATCAAGTG GATTGTGACC 
ATAGGTGCTG TGTTTGCGCT CTGCACTAGT TTGTTGGGCG CCATGTTCCC ACTGCCTCGC 
ATCCTCTACG CCATGGGCAA GGACGGCATA TTGTTCAAGA GGCTGTCCAC AGTGAACAGC 
TACACGAAGA CCCCGCTCCT GGCCACCATT GTGTCTGGAA TCTTTGCGTC CATAATGGCC 
ATGCTCTTTA ACCTGGACCA GCTTGTGGAC ATGATGTCCA TCGGAACCCT GTTAGCATAC 
ACCATCGTGG CAATTTGCGT GCTAGTGCTG CGCTACCAGG ACGAGGAGAT GACCAAGCTG 
GTGTCTGTCA AGGCACCGAA TGTTTTCCGG CAATTCTTCA ACGGCAACTC CTTCCGGGAG 
CCCAATTCTA TGACCTCGTC CATCACCAAA GTCGGCATTG TGGTGTTCGC CATCTTTTGC 
CTGGTCTGGT GCTCCCTCCA GAAAGTCTTC GACCTGGACA GCACCGGCGG AATTGTGGCG 
CTCAGCTTGG TGGGCGCTGT GCTAATCCTC ATCTGCGTTG TGATCGGCAT GCAGCCGGTG 
TCGACCATTG AGCTCACGTT CAAGGTGCCG CTAGTGCCGT TCGTGCCGTG CCTCAGCGTC 
TTTGCGAATT TGTACCTGAT GTTTCAGCTG GACCTGAACA CATGGATCCG CTTCCTCATC 
TGGATCGTGA TCGGCTACGT GATTTACTTC TGTTACGGCA TGCGGAACTC CACGCAGATA 
AGTAGGAGCC GCAGTCACGC CGAGGTGGCG GCAAGCGCTC TGCAGAACCA GGGACAGCAC 
GTGAATCCCG GCTTCGAGCC GGACTACAAG GTCGAAAATG GGGGTCGACC CTACGAGTTC 
TCCGAGAAGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730764.1
CDS	357..2171
Translation	MTNLWKALTRRKTEDVNEGESQLARVLNLFDLTALGVGSTLGLGVYVLAGQVAFNIAGPAVTISFLIAAIASAFAGICYAEFAARVPKAGSAYVYSYVTIGEFVAFTIGWNLILEYVIGTASVARGLSGYFDSLINNNMSKALNESMHIDVDFLGDYPDFLSFGMVLLLAAILAFGAKESSFLNNIFTTVNLVTIAIVLVAGAMNANVDNWRIPKKDVPEGFGTGGFMPFGIAGVMAGAAKCFYGFVGFDCIATTGEEAINPKRNIPLSIVVSLIIIFLSYFGVSTVLTMMLPYYKQDKDAPFPHAFDSVEWYTIKWIVTIGAVFALCTSLLGAMFPLPRILYAMGKDGILFKRLSTVNSYTKTPLLATIVSGIFASIMAMLFNLDQLVDMMSIGTLLAYTIVAICVLVLRYQDEEMTKLVSVKAPNVFRQFFNGNSFREPNSMTSSITKVGIVVFAIFCLVWCSLQKVFDLDSTGGIVALSLVGAVLILICVVIGMQPVSTIELTFKVPLVPFVPCLSVFANLYLMFQLDLNTWIRFLIWIVIGYVIYFCYGMRNSTQISRSRSHAEVAASALQNQGQHVNPGFEPDYKVENGGRPYEFSEKL

Target ORF information:

RefSeq Version	NM_168977.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slimfast (slif), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168977.2

ORF Insert Sequence:

ATGACCAATC TCTGGAAGGC ATTGACGCGT CGCAAGACGG AGGACGTGAA CGAAGGCGAG 
TCCCAGTTGG CGCGAGTGCT GAACCTTTTC GATCTGACGG CTTTGGGAGT GGGCAGTACT 
TTGGGACTGG GAGTCTATGT GCTGGCTGGT CAGGTGGCCT TCAATATTGC CGGGCCAGCG 
GTGACCATTT CCTTCCTGAT TGCGGCCATC GCGTCTGCCT TCGCGGGGAT CTGCTATGCG 
GAGTTCGCCG CGCGAGTGCC CAAGGCGGGA AGCGCCTACG TATACAGCTA CGTGACCATT 
GGAGAGTTCG TCGCCTTCAC CATAGGATGG AATCTCATTC TGGAGTATGT GATTGGCACA 
GCCTCAGTTG CGCGCGGCCT GAGCGGATAT TTCGACTCTC TGATCAACAA CAACATGTCG 
AAGGCATTGA ATGAGTCGAT GCACATCGAC GTCGACTTTC TGGGTGACTA CCCCGATTTT 
TTGTCCTTCG GCATGGTGCT GCTCCTGGCG GCCATCCTCG CCTTTGGCGC CAAAGAGTCA 
AGCTTCCTGA ACAACATCTT CACCACAGTC AATCTGGTTA CGATCGCTAT CGTCCTGGTG 
GCTGGCGCCA TGAACGCCAA CGTCGACAAT TGGCGCATCC CGAAGAAAGA TGTTCCCGAG 
GGCTTTGGCA CGGGAGGCTT CATGCCGTTC GGCATTGCTG GAGTGATGGC CGGAGCTGCC 
AAATGCTTCT ACGGCTTCGT GGGGTTCGAC TGCATCGCCA CCACGGGCGA GGAGGCCATC 
AACCCAAAGC GCAACATTCC GCTCTCTATC GTGGTGTCGC TGATCATAAT CTTCCTATCC 
TACTTTGGAG TGTCCACGGT GCTGACTATG ATGCTGCCCT ACTACAAACA GGACAAGGAT 
GCACCTTTTC CTCACGCTTT CGATTCCGTC GAGTGGTACA CCATCAAGTG GATTGTGACC 
ATAGGTGCTG TGTTTGCGCT CTGCACTAGT TTGTTGGGCG CCATGTTCCC ACTGCCTCGC 
ATCCTCTACG CCATGGGCAA GGACGGCATA TTGTTCAAGA GGCTGTCCAC AGTGAACAGC 
TACACGAAGA CCCCGCTCCT GGCCACCATT GTGTCTGGAA TCTTTGCGTC CATAATGGCC 
ATGCTCTTTA ACCTGGACCA GCTTGTGGAC ATGATGTCCA TCGGAACCCT GTTAGCATAC 
ACCATCGTGG CAATTTGCGT GCTAGTGCTG CGCTACCAGG ACGAGGAGAT GACCAAGCTG 
GTGTCTGTCA AGGCACCGAA TGTTTTCCGG CAATTCTTCA ACGGCAACTC CTTCCGGGAG 
CCCAATTCTA TGACCTCGTC CATCACCAAA GTCGGCATTG TGGTGTTCGC CATCTTTTGC 
CTGGTCTGGT GCTCCCTCCA GAAAGTCTTC GACCTGGACA GCACCGGCGG AATTGTGGCG 
CTCAGCTTGG TGGGCGCTGT GCTAATCCTC ATCTGCGTTG TGATCGGCAT GCAGCCGGTG 
TCGACCATTG AGCTCACGTT CAAGGTGCCG CTAGTGCCGT TCGTGCCGTG CCTCAGCGTC 
TTTGCGAATT TGTACCTGAT GTTTCAGCTG GACCTGAACA CATGGATCCG CTTCCTCATC 
TGGATCGTGA TCGGCTACGT GATTTACTTC TGTTACGGCA TGCGGAACTC CACGCAGATA 
AGTAGGAGCC GCAGTCACGC CGAGGTGGCG GCAAGCGCTC TGCAGAACCA GGGACAGCAC 
GTGAATCCCG GCTTCGAGCC GGACTACAAG GTCGAAAATG GGGGTCGACC CTACGAGTTC 
TCCGAGAAGC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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slif cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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