l(3)72Dn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	l(3)72Dn
Entrez Gene ID	39774
Full Name	lethal (3) 72Dn
Synonyms	CG5018,Dmel\CG5018,l(3)j5A4
General protein information	Preferred Names lethal (3) 72Dn Names CG5018 gene product from transcript CG5018-RA CG5018-PA CG5018-PB l(3)72Dn-PA l(3)72Dn-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 72D10-72D10

mRNA and Protein(s)

mRNA	Protein	Name
NM_140589.3	NP_648846.2	lethal (3) 72Dn, isoform A
NM_001275001.1	NP_001261930.1	lethal (3) 72Dn, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	CIRH1A	NP_116219.2
Danio rerio (zebrafish)	cirh1a	NP_998447.1
Arabidopsis thaliana (thale cress)	AT4G07410	NP_567317.2
Oryza sativa (rice)	Os03g0735100	NP_001051185.2
Rattus norvegicus (Norway rat)	Cirh1a	NP_001009640.1
Kluyveromyces lactis	KLLA0D13222g	XP_453652.1
Macaca mulatta (Rhesus monkey)	CIRH1A	XP_001093408.2
Canis lupus familiaris (dog)	CIRH1A	XP_546858.3
Bos taurus (cattle)	CIRH1A	NP_001192601.1
Saccharomyces cerevisiae (baker's yeast)	UTP4	NP_010611.3
Schizosaccharomyces pombe (fission yeast)	utp4	NP_596540.1
Eremothecium gossypii	AGOS_AGR367C	NP_987033.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006092	XP_316150.4
Arabidopsis thaliana (thale cress)	AT1G27470	NP_174067.2
Xenopus tropicalis (tropical clawed frog)	cirh1a	NP_001039163.1
Pan troglodytes (chimpanzee)	CIRH1A	XP_511062.3
Mus musculus (house mouse)	Cirh1a	NP_035704.2
Gallus gallus (chicken)	CIRH1A	XP_004944391.1
Drosophila melanogaster (fruit fly)	l(3)72Dn	NP_001261930.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following l(3)72Dn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the l(3)72Dn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18642	NM_140589.3 Latest version!	Drosophila melanogaster lethal (3) 72Dn (l(3)72Dn), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa20985	NM_001275001.1 Latest version!	Drosophila melanogaster lethal (3) 72Dn (l(3)72Dn), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

l(3)72Dn ( NM_140589.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648846.2 Drosophila melanogaster lethal (3) 72Dn (l(3)72Dn), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18642
Clone ID Related Accession (Same CDS sequence)	NM_140589.3
Accession Version	NM_140589.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (3) 72Dn, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140589.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGAATA ATGCGAAGAA GGAACCCCAG CAAAGGGGCA AGCATCAGGT GCATAATGTG 
CGCTTCTACA CTATAAAGCC GCGTGAAATT GTCAGCTTGG CGTATAGTAA ATCGAGCAAG 
TGCCTCGCTC TGAGTCGTGC AACACCCGTC ATTGAGCTAT GGAACCTGGA GCACGCCCCG 
TATTTGGATC GTGTAATCCA CCTGCCCACC GATTCTCATG TGGAATCTAT AGCCTGGGCG 
GGAAACCGCT TATTTTCCGT GGATTTAAGT GGTAAACTGA TCGAGTGGGA TGTGATTAAG 
CTAAAACAAC GATATGAGCA CTCGCCCACT GGCAATGCTC TCTGGAGTAT CGATGTGAAT 
CCCGCGGAAA CGGATATTGC CATTGGCTCA GAGGAAGGGC ACATCAACAT CCTGAGTATT 
GAGAATGACG AAATCACCTA TAAATCCCTA TTTAACAAGC AAAAAGGTCG CGTTCTCTGC 
ATCAAGTTTG ATAAGACTGG AACTAAACTG GTGACCGGCA CTGAGGGATT TGTTCGCATT 
TGGAACGTCC TCAAGGGAAC CACTCTGCAC ACGATGACCC TATCCGAAAA GAACGTAATA 
GTTTGGTCCC TGCAGGTGCT ATCCGATAAT ACGATTATTG CCGGTGATTC AGCGGGATTT 
GTTACCGTTT GGGATGCGGA TAACGCGACA CAGATAGATT CCACACGAGT TCTGGATAAA 
AACGTATTTG CCCTGGCTGT GAATAATAAG GAGGATCGAT TAGTTTGCAG TGGAATGCAG 
CCGCCGCTCA TCCGGATTTT CAGCAAAACC AAGATAAAGC GGGAAGAGTC CACCAGCGAG 
CGTTGGATTA AATTCCTACA GCGCGATGCC CACAAACACT ATGTGAAATC CCTGTTGGTT 
ATCGACGATC AAATTTATTC GGGAGGCAGG GATGGCATAT TGACCATTAC GTCCAGTGAA 
CGAATGCAGG CACATTTGTC GCAGCATGCT CCCTTTCTGA AAGGTTCCGT AGCTTCCATG 
GCAATCAGTA AAAAGCTTTT GCTTTTGCGA TATCCCAACA GCGTGCACCT GTGGCGCTTG 
GGTTCTGTGG CCCCGCAAGG CGAGGACAAG AAGAAACCCT GGGCACTGCC CGTCGGCCAC 
ACGGAGGAAC AACTTCTGGA GCTGCCACCC CAGAAACTTC TCCAACTAAA TGTCAAAGAG 
CACAACTTTA TACAGTCTGC TGCCATTTCA TCAGATGCAA ACTGGATTTG CTACTCTACC 
TTAAAGGAAC TCCGAATCAG TCGTTTGAAA AGTGATCCCC TGCAGGTGGA GCGTCTGGTA 
GACGATCTTC CCGAGGAACT TCAACCAGCC AGTCATATAA TTTTCACAAA GCAGGGAGAT 
CTAGTGCTGC TCAACCCGCA GAACAATCAT CTAAGTTGGT TTACTTTAGA GGAAGATCTG 
GTTACATTCA AATATACCAT CGATTTGAGT GAGAAGTGTA AGAATACGAT TAGCCACGTG 
GTTATCTCTT CGCATGGCGA ATACATTGTG GCTGCGAGCT CAGACCACAT AATATCAGTT 
TGGAAACTAC ATGGAAAACA ATACAAGCAC CTGCTGAACC TACCAAGACA CCGAGCAGGT 
ACCACCGCCC TATCCATGCA CGAGGATTAC CCACGCGTGG TGGTCGCCTA CGCCAATGGC 
CAGCTGGTGG AGTACGATTT GGTCAATCGG ATATTCACCT GTGAAACTAA TGAGTACCTG 
ATACCCGAGA CCAGGCGCCA TTGCATCAAT GGAATCAGCC TGGATCCACA AAATCGTAAT 
ATATTTATTG TTCACACCGA GGGCAATTTG TATGTGTTGG AGAGGGATCA GCATCTGGAT 
CCCAAAGAGC TTGTGAGCAA CAGCAAGTCT AAAAAGTTAT CAAATGGAAA CCGCTCTTCA 
GTTGATGGCG GCTCAAAAGG CCTGAGCCTC AAGACGACTC TGTCACGACA GCACCTGGTG 
CACGTGTCTC GCCTGAGCCC TAACGAACTT GTCAACGTCA GCATTTCGAC AAACAATCTG 
TTGGCTCCGC TGCCGCCGCC GTACCAGCGG AAGAAGTTCG GTGCCTCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648846.2
CDS	111..2201
Translation	MTNNAKKEPQQRGKHQVHNVRFYTIKPREIVSLAYSKSSKCLALSRATPVIELWNLEHAPYLDRVIHLPTDSHVESIAWAGNRLFSVDLSGKLIEWDVIKLKQRYEHSPTGNALWSIDVNPAETDIAIGSEEGHINILSIENDEITYKSLFNKQKGRVLCIKFDKTGTKLVTGTEGFVRIWNVLKGTTLHTMTLSEKNVIVWSLQVLSDNTIIAGDSAGFVTVWDADNATQIDSTRVLDKNVFALAVNNKEDRLVCSGMQPPLIRIFSKTKIKREESTSERWIKFLQRDAHKHYVKSLLVIDDQIYSGGRDGILTITSSERMQAHLSQHAPFLKGSVASMAISKKLLLLRYPNSVHLWRLGSVAPQGEDKKKPWALPVGHTEEQLLELPPQKLLQLNVKEHNFIQSAAISSDANWICYSTLKELRISRLKSDPLQVERLVDDLPEELQPASHIIFTKQGDLVLLNPQNNHLSWFTLEEDLVTFKYTIDLSEKCKNTISHVVISSHGEYIVAASSDHIISVWKLHGKQYKHLLNLPRHRAGTTALSMHEDYPRVVVAYANGQLVEYDLVNRIFTCETNEYLIPETRRHCINGISLDPQNRNIFIVHTEGNLYVLERDQHLDPKELVSNSKSKKLSNGNRSSVDGGSKGLSLKTTLSRQHLVHVSRLSPNELVNVSISTNNLLAPLPPPYQRKKFGAS

Target ORF information:

RefSeq Version	NM_140589.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (3) 72Dn (l(3)72Dn), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140589.3

ORF Insert Sequence:

ATGACGAATA ATGCGAAGAA GGAACCCCAG CAAAGGGGCA AGCATCAGGT GCATAATGTG 
CGCTTCTACA CTATAAAGCC GCGTGAAATT GTCAGCTTGG CGTATAGTAA ATCGAGCAAG 
TGCCTCGCTC TGAGTCGTGC AACACCCGTC ATTGAGCTAT GGAACCTGGA GCACGCCCCG 
TATTTGGATC GTGTAATCCA CCTGCCCACC GATTCTCATG TGGAATCTAT AGCCTGGGCG 
GGAAACCGCT TATTTTCCGT GGATTTAAGT GGTAAACTGA TCGAGTGGGA TGTGATTAAG 
CTAAAACAAC GATATGAGCA CTCGCCCACT GGCAATGCTC TCTGGAGTAT CGATGTGAAT 
CCCGCGGAAA CGGATATTGC CATTGGCTCA GAGGAAGGGC ACATCAACAT CCTGAGTATT 
GAGAATGACG AAATCACCTA TAAATCCCTA TTTAACAAGC AAAAAGGTCG CGTTCTCTGC 
ATCAAGTTTG ATAAGACTGG AACTAAACTG GTGACCGGCA CTGAGGGATT TGTTCGCATT 
TGGAACGTCC TCAAGGGAAC CACTCTGCAC ACGATGACCC TATCCGAAAA GAACGTAATA 
GTTTGGTCCC TGCAGGTGCT ATCCGATAAT ACGATTATTG CCGGTGATTC AGCGGGATTT 
GTTACCGTTT GGGATGCGGA TAACGCGACA CAGATAGATT CCACACGAGT TCTGGATAAA 
AACGTATTTG CCCTGGCTGT GAATAATAAG GAGGATCGAT TAGTTTGCAG TGGAATGCAG 
CCGCCGCTCA TCCGGATTTT CAGCAAAACC AAGATAAAGC GGGAAGAGTC CACCAGCGAG 
CGTTGGATTA AATTCCTACA GCGCGATGCC CACAAACACT ATGTGAAATC CCTGTTGGTT 
ATCGACGATC AAATTTATTC GGGAGGCAGG GATGGCATAT TGACCATTAC GTCCAGTGAA 
CGAATGCAGG CACATTTGTC GCAGCATGCT CCCTTTCTGA AAGGTTCCGT AGCTTCCATG 
GCAATCAGTA AAAAGCTTTT GCTTTTGCGA TATCCCAACA GCGTGCACCT GTGGCGCTTG 
GGTTCTGTGG CCCCGCAAGG CGAGGACAAG AAGAAACCCT GGGCACTGCC CGTCGGCCAC 
ACGGAGGAAC AACTTCTGGA GCTGCCACCC CAGAAACTTC TCCAACTAAA TGTCAAAGAG 
CACAACTTTA TACAGTCTGC TGCCATTTCA TCAGATGCAA ACTGGATTTG CTACTCTACC 
TTAAAGGAAC TCCGAATCAG TCGTTTGAAA AGTGATCCCC TGCAGGTGGA GCGTCTGGTA 
GACGATCTTC CCGAGGAACT TCAACCAGCC AGTCATATAA TTTTCACAAA GCAGGGAGAT 
CTAGTGCTGC TCAACCCGCA GAACAATCAT CTAAGTTGGT TTACTTTAGA GGAAGATCTG 
GTTACATTCA AATATACCAT CGATTTGAGT GAGAAGTGTA AGAATACGAT TAGCCACGTG 
GTTATCTCTT CGCATGGCGA ATACATTGTG GCTGCGAGCT CAGACCACAT AATATCAGTT 
TGGAAACTAC ATGGAAAACA ATACAAGCAC CTGCTGAACC TACCAAGACA CCGAGCAGGT 
ACCACCGCCC TATCCATGCA CGAGGATTAC CCACGCGTGG TGGTCGCCTA CGCCAATGGC 
CAGCTGGTGG AGTACGATTT GGTCAATCGG ATATTCACCT GTGAAACTAA TGAGTACCTG 
ATACCCGAGA CCAGGCGCCA TTGCATCAAT GGAATCAGCC TGGATCCACA AAATCGTAAT 
ATATTTATTG TTCACACCGA GGGCAATTTG TATGTGTTGG AGAGGGATCA GCATCTGGAT 
CCCAAAGAGC TTGTGAGCAA CAGCAAGTCT AAAAAGTTAT CAAATGGAAA CCGCTCTTCA 
GTTGATGGCG GCTCAAAAGG CCTGAGCCTC AAGACGACTC TGTCACGACA GCACCTGGTG 
CACGTGTCTC GCCTGAGCCC TAACGAACTT GTCAACGTCA GCATTTCGAC AAACAATCTG 
TTGGCTCCGC TGCCGCCGCC GTACCAGCGG AAGAAGTTCG GTGCCTCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

l(3)72Dn ( NM_001275001.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261930.1 Drosophila melanogaster lethal (3) 72Dn (l(3)72Dn), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa20985
Clone ID Related Accession (Same CDS sequence)	NM_001275001.1
Accession Version	NM_001275001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (3) 72Dn, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGAATA ATGCGAAGAA GGAACCCCAG CAAAGGGGCA AGCATCAGGT GCATAATGTG 
CGCTTCTACA CTATAAAGCC GCGTGAAATT GTCAGCTTGG CGTATAGTAA ATCGAGCAAG 
TGCCTCGCTC TGAGTCGTGC AACACCCGTC ATTGAGCTAT GGAACCTGGA GCACGCCCCG 
TATTTGGATC GTGTAATCCA CCTGCCCACC GATTCTCATG TGGAATCTAT AGCCTGGGCG 
GGAAACCGCT TATTTTCCGT GGATTTAAGT GGTAAACTGA TCGAGTGGGA TGTGATTAAG 
CTAAAACAAC GATATGAGCA CTCGCCCACT GGCAATGCTC TCTGGAGTAT CGATGTGAAT 
CCCGCGGAAA CGGATATTGC CATTGGCTCA GAGGAAGGGC ACATCAACAT CCTGAGTATT 
GAGAATGACG AAATCACCTA TAAATCCCTA TTTAACAAGC AAAAAGGTCG CGTTCTCTGC 
ATCAAGTTTG ATAAGACTGG AACTAAACTG GTGACCGGCA CTGAGGGATT TGTTCGCATT 
TGGAACGTCC TCAAGGGAAC CACTCTGCAC ACGATGACCC TATCCGAAAA GAACGTAATA 
GTTTGGTCCC TGCAGGTGCT ATCCGATAAT ACGATTATTG CCGGTGATTC AGCGGGATTT 
GTTACCGTTT GGGATGCGGA TAACGCGACA CAGATAGATT CCACACGAGT TCTGGATAAA 
AACGTATTTG CCCTGGCTGT GAATAATAAG GAGGATCGAT TAGTTTGCAG TGGAATGCAG 
CCGCCGCTCA TCCGGATTTT CAGCAAAACC AAGATAAAGC GGGAAGAGTC CACCAGCGAG 
CGTTGGATTA AATTCCTACA GCGCGATGCC CACAAACACT ATGTGAAATC CCTGTTGGTT 
ATCGACGATC AAATTTATTC GGGAGGCAGG GATGGCATAT TGACCATTAC GTCCAGTGAA 
CGAATGCAGG CACATTTGTC GCAGCATGCT CCCTTTCTGA AAGGTTCCGT AGCTTCCATG 
GCAATCAGTA AAAAGCTTTT GCTTTTGCGA TATCCCAACA GCGTGCACCT GTGGCGCTTG 
GGTTCTGTGG CCCCGCAAGG CGAGGACAAG AAGAAACCCT GGGCACTGCC CGTCGGCCAC 
ACGGAGGAAC AACTTCTGGA GCTGCCACCC CAGAAACTTC TCCAACTAAA TGTCAAAGAG 
CACAACTTTA TACAGTCTGC TGCCATTTCA TCAGATGCAA ACTGGATTTG CTACTCTACC 
TTAAAGGAAC TCCGAATCAG TCGTTTGAAA AGTGATCCCC TGCAGGTGGA GCGTCTGGTA 
GACGATCTTC CCGAGGAACT TCAACCAGCC AGTCATATAA TTTTCACAAA GCAGGGAGAT 
CTAGTGCTGC TCAACCCGCA GAACAATCAT CTAAGTTGGT TTACTTTAGA GGAAGATCTG 
GTTACATTCA AATATACCAT CGATTTGAGT GAGAAGTGTA AGAATACGAT TAGCCACGTG 
GTTATCTCTT CGCATGGCGA ATACATTGTG GCTGCGAGCT CAGACCACAT AATATCAGTT 
TGGAAACTAC ATGGAAAACA ATACAAGCAC CTGCTGAACC TACCAAGACA CCGAGCAGGT 
ACCACCGCCC TATCCATGCA CGAGGATTAC CCACGCGTGG TGGTCGCCTA CGCCAATGGC 
CAGCTGGTGG AGTACGATTT GGTCAATCGG ATATTCACCT GTGAAACTAA TGAGTACCTG 
ATACCCGAGA CCAGGCGCCA TTGCATCAAT GGAATCAGCC TGGATCCACA AAATCGTAAT 
ATATTTATTG TTCACACCGA GGGCAATTTG TATGTGTTGG AGAGGGATCA GCATCTGGAT 
CCCAAAGAGC TTGTGAGCAA CAGCAAGTCT AAAAAGTTAT CAAATGGAAA CCGCTCTTCA 
GTTGATGGCG GCTCAAAAGG CCTGAGCCTC AAGACGACTC TGTCACGACA GGTAAGATTG 
CACCTGGTGC ACGTGTCTCG CCTGAGCCCT AACGAACTTG TCAACGTCAG CATTTCGACA 
AACAATCTGT TGGCTCCGCT GCCGCCGCCG TACCAGCGGA AGAAGTTCGG TGCCTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261930.1
CDS	111..2210
Translation	MTNNAKKEPQQRGKHQVHNVRFYTIKPREIVSLAYSKSSKCLALSRATPVIELWNLEHAPYLDRVIHLPTDSHVESIAWAGNRLFSVDLSGKLIEWDVIKLKQRYEHSPTGNALWSIDVNPAETDIAIGSEEGHINILSIENDEITYKSLFNKQKGRVLCIKFDKTGTKLVTGTEGFVRIWNVLKGTTLHTMTLSEKNVIVWSLQVLSDNTIIAGDSAGFVTVWDADNATQIDSTRVLDKNVFALAVNNKEDRLVCSGMQPPLIRIFSKTKIKREESTSERWIKFLQRDAHKHYVKSLLVIDDQIYSGGRDGILTITSSERMQAHLSQHAPFLKGSVASMAISKKLLLLRYPNSVHLWRLGSVAPQGEDKKKPWALPVGHTEEQLLELPPQKLLQLNVKEHNFIQSAAISSDANWICYSTLKELRISRLKSDPLQVERLVDDLPEELQPASHIIFTKQGDLVLLNPQNNHLSWFTLEEDLVTFKYTIDLSEKCKNTISHVVISSHGEYIVAASSDHIISVWKLHGKQYKHLLNLPRHRAGTTALSMHEDYPRVVVAYANGQLVEYDLVNRIFTCETNEYLIPETRRHCINGISLDPQNRNIFIVHTEGNLYVLERDQHLDPKELVSNSKSKKLSNGNRSSVDGGSKGLSLKTTLSRQVRLHLVHVSRLSPNELVNVSISTNNLLAPLPPPYQRKKFGAS

Target ORF information:

RefSeq Version	NM_001275001.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (3) 72Dn (l(3)72Dn), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275001.1

ORF Insert Sequence:

ATGACGAATA ATGCGAAGAA GGAACCCCAG CAAAGGGGCA AGCATCAGGT GCATAATGTG 
CGCTTCTACA CTATAAAGCC GCGTGAAATT GTCAGCTTGG CGTATAGTAA ATCGAGCAAG 
TGCCTCGCTC TGAGTCGTGC AACACCCGTC ATTGAGCTAT GGAACCTGGA GCACGCCCCG 
TATTTGGATC GTGTAATCCA CCTGCCCACC GATTCTCATG TGGAATCTAT AGCCTGGGCG 
GGAAACCGCT TATTTTCCGT GGATTTAAGT GGTAAACTGA TCGAGTGGGA TGTGATTAAG 
CTAAAACAAC GATATGAGCA CTCGCCCACT GGCAATGCTC TCTGGAGTAT CGATGTGAAT 
CCCGCGGAAA CGGATATTGC CATTGGCTCA GAGGAAGGGC ACATCAACAT CCTGAGTATT 
GAGAATGACG AAATCACCTA TAAATCCCTA TTTAACAAGC AAAAAGGTCG CGTTCTCTGC 
ATCAAGTTTG ATAAGACTGG AACTAAACTG GTGACCGGCA CTGAGGGATT TGTTCGCATT 
TGGAACGTCC TCAAGGGAAC CACTCTGCAC ACGATGACCC TATCCGAAAA GAACGTAATA 
GTTTGGTCCC TGCAGGTGCT ATCCGATAAT ACGATTATTG CCGGTGATTC AGCGGGATTT 
GTTACCGTTT GGGATGCGGA TAACGCGACA CAGATAGATT CCACACGAGT TCTGGATAAA 
AACGTATTTG CCCTGGCTGT GAATAATAAG GAGGATCGAT TAGTTTGCAG TGGAATGCAG 
CCGCCGCTCA TCCGGATTTT CAGCAAAACC AAGATAAAGC GGGAAGAGTC CACCAGCGAG 
CGTTGGATTA AATTCCTACA GCGCGATGCC CACAAACACT ATGTGAAATC CCTGTTGGTT 
ATCGACGATC AAATTTATTC GGGAGGCAGG GATGGCATAT TGACCATTAC GTCCAGTGAA 
CGAATGCAGG CACATTTGTC GCAGCATGCT CCCTTTCTGA AAGGTTCCGT AGCTTCCATG 
GCAATCAGTA AAAAGCTTTT GCTTTTGCGA TATCCCAACA GCGTGCACCT GTGGCGCTTG 
GGTTCTGTGG CCCCGCAAGG CGAGGACAAG AAGAAACCCT GGGCACTGCC CGTCGGCCAC 
ACGGAGGAAC AACTTCTGGA GCTGCCACCC CAGAAACTTC TCCAACTAAA TGTCAAAGAG 
CACAACTTTA TACAGTCTGC TGCCATTTCA TCAGATGCAA ACTGGATTTG CTACTCTACC 
TTAAAGGAAC TCCGAATCAG TCGTTTGAAA AGTGATCCCC TGCAGGTGGA GCGTCTGGTA 
GACGATCTTC CCGAGGAACT TCAACCAGCC AGTCATATAA TTTTCACAAA GCAGGGAGAT 
CTAGTGCTGC TCAACCCGCA GAACAATCAT CTAAGTTGGT TTACTTTAGA GGAAGATCTG 
GTTACATTCA AATATACCAT CGATTTGAGT GAGAAGTGTA AGAATACGAT TAGCCACGTG 
GTTATCTCTT CGCATGGCGA ATACATTGTG GCTGCGAGCT CAGACCACAT AATATCAGTT 
TGGAAACTAC ATGGAAAACA ATACAAGCAC CTGCTGAACC TACCAAGACA CCGAGCAGGT 
ACCACCGCCC TATCCATGCA CGAGGATTAC CCACGCGTGG TGGTCGCCTA CGCCAATGGC 
CAGCTGGTGG AGTACGATTT GGTCAATCGG ATATTCACCT GTGAAACTAA TGAGTACCTG 
ATACCCGAGA CCAGGCGCCA TTGCATCAAT GGAATCAGCC TGGATCCACA AAATCGTAAT 
ATATTTATTG TTCACACCGA GGGCAATTTG TATGTGTTGG AGAGGGATCA GCATCTGGAT 
CCCAAAGAGC TTGTGAGCAA CAGCAAGTCT AAAAAGTTAT CAAATGGAAA CCGCTCTTCA 
GTTGATGGCG GCTCAAAAGG CCTGAGCCTC AAGACGACTC TGTCACGACA GGTAAGATTG 
CACCTGGTGC ACGTGTCTCG CCTGAGCCCT AACGAACTTG TCAACGTCAG CATTTCGACA 
AACAATCTGT TGGCTCCGCT GCCGCCGCCG TACCAGCGGA AGAAGTTCGG TGCCTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
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Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
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Combined evidence annotation of transposable elements in genome sequences.
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