Ube3a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube3a
Entrez Gene ID	39266
Full Name	Ubiquitin protein ligase E3A
Synonyms	AS,As,CG6190,Dmel\CG6190,Dube3A,Dube3a,NM_140195.2,UBE3A,dUBE3A,dUbe3a,das,dube3A,dube3a,ube3a
General protein information	Preferred Names Ubiquitin protein ligase E3A Names CG6190 gene product from transcript CG6190-RA CG6190-PA Ube3a-PA angelman syndrome drosophila angelman syndrome ubiquitin protein ligase E3A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 68B1-68B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_140195.4	NP_648452.1	ubiquitin protein ligase E3A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ube3a	NP_035798.2
Rattus norvegicus (Norway rat)	Ube3a	NP_001178766.1
Danio rerio (zebrafish)	ube3a	NP_001007319.1
Pan troglodytes (chimpanzee)	UBE3A	XP_001161311.1
Bos taurus (cattle)	UBE3A	NP_001091932.1
Gallus gallus (chicken)	UBE3A	XP_004938531.1
Drosophila melanogaster (fruit fly)	Ube3a	NP_648452.1
Homo sapiens (human)	UBE3A	NP_570854.1
Macaca mulatta (Rhesus monkey)	UBE3A	XP_001108661.1
Canis lupus familiaris (dog)	UBE3A	XP_005618288.1
Xenopus tropicalis (tropical clawed frog)	ube3a	NP_001001213.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012366	XP_551819.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ube3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18232	NM_140195.4 Latest version!	Drosophila melanogaster ubiquitin protein ligase E3A (Ube3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube3a ( NM_140195.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648452.1 Drosophila melanogaster ubiquitin protein ligase E3A (Ube3a), mRNA.

Price & Availability↑

CloneID	OFa18232
Clone ID Related Accession (Same CDS sequence)	NM_140195.4
Accession Version	NM_140195.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin protein ligase E3A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140195.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGGTG GCGGGGGTGG CGAGGATGAT CAGCATCTGC CAGGTGTTTC CAGTGCATCA 
GGATCTGCAG CTGGAGCGGT GGGCGGGCGA GCCACGCCCG AAATGAAGCG TTCGGCGGTG 
CGCAGCCTAA TCAAGCGATA CTTCCACCAA CTACAATCCG GCTGTGGGAA CGCCAACTGC 
AGCAATGCCA ATTGCGCCTC CAGCGGCAAG GTGGCGCCCA TGACGCCCAA CGAGGTGGCC 
GCCAGAGCAC TGCAGCTCTT TTCACAGGAT GCACAGCTCT GCGAGGCGTT CACATCGGAG 
GCCAACAGCC CCCAGGACGT GGACATGCTT TCGCCGAACG ACAGTAGCAG TAGTAGCAGC 
GGCAGCTCGA CAAGCACAAT TACCTCAGCC AGTACCACCA CGACAACCAG CAGACAATCG 
CAGTCAACGC CAGCTGCCGT TGTAGTGCCC GTATCCAGTC CATATCTTAC CCAGAGTGTG 
CCGCAACTGG ATATCGCCGG CGCTGAACAC TCCTCCGGCG ACTCGGAACC GTGTACACCC 
ACCCTGGCGC CAGTCCACAG CTTGGATGCC AACAGTCTGA TAGCGCTCTA TGAGCAATGT 
AGGGCGGCTG ACAGTTACGA CAGGATTTGC CACGCAATCG GCGTTGTGTT CTCCAGCGTA 
GATAGACTGG GCAAGAGCTT CATTCGCAGC GCAGAACCAG CGGCTTCTAG TAGTCTCCAG 
GAGCTGCTGG CCAATTCACC GGGGGCTTTA AACAAGGAGC AGCTACGAAC TTTGGAAGGG 
GAACACGATA AAGACGAAGA CAGTACACAA CAGGTGGACG AACAATCAGA ATCGGCGGCT 
AATGCTACTG CTACTGCAGA ATCAGTCGAA TCAGAGGCGG AGCCCGAAGA AGAGGACGAT 
GATGTGTGTT CATCCCAAAG CAGCGACACA CTGGTGGATC TGCCCGGTTT GCGACGAGTG 
CAGCGTCTCC TTTTTGGCTG TCAAATACGA GCTATAACGG AGAAGCTTAC CAGCAGCGTT 
ATTCAGCTGT CTGACTGGGT GATTTACATG CGTACGGATT GGGAGAGGGT CATTCACTGT 
CTGGTCATCT GCTTTGATCT AGCCACCAAT ACCAATAACA GTGTGGTGGA CATGGACTAT 
CTGGACAGGG TGCTGCCAAA ATTGTGCCAA GCGGCGTCGG CCATGCCAGT GCCGGCACAG 
GCGCGCCTGG CCAGGATCTG GGCTGCTCAT TGTTCAGATC AGCTGCATTC GTTGATCGCA 
GCCTGCCAGC AGCAAATTAC TCTACAGGTG CTTTTGGACG AGGAATCCAT GCGCGAGAAC 
GAATCCATAA TCAGTGTCAC CAAAGTTTTA AAGATTGTCT TCTACGCGAA CATTCTGGCT 
AGCGAGCTGG AGCGACCGTC GTGTCGGGTG CCGCTGGAGG ATCGAACGGA AGCGGCGACC 
GCATCTGGCA GCGCAGCAGT GGAGGACGAC CTATTCGTCT ACAACTCTTT GTTGGAGCCG 
CACATGCCCA AATTCGCCGA GGATCAGTTT GAAAAGGAGC TGCAGGTGTC GGCAATCGAT 
TGCCGGAAAC CACTGATACC ACTTGAGGAG TTCTACAACG AGGCACTCAG TGAAAACATT 
CAGATGCACC ACGACTACCT GTCCTACAAG ACGTTAGCCA TGGAGAGCGA GATTGGTTCC 
GGCCACGCGA ACTATTTTTC GTTCATGCTG TACGCCTTTA TCCTCACGCC GTCAACAAAA 
GTGGATGCCT TGTACTATGA CAGTCGAATG AGGATGTATA GCGAGCGCTA CTCCTCCCTT 
CACTCCATCC TCAACAATAT TGGCCAGGTA GGACAGGAAG ATAATCCTCG GCCGGATCTT 
AAGCTGACGG TGCGAAGGGA CCAGCTCATC AACGACGCAC TTATTGGGCT GGAAATGGTG 
GCCATGAGCA ATCCAAAGGA TCTCAAGAAG CAGTTGGTGG TGGAGTTTGT CGGCGAGCAG 
GGGATCGATG AAGGCGGCGT ATCCAAAGAG TTCTTCCAGC TAATCGTGGA GGAGATATTT 
AACCCGGCTT TTGGAATGTT TATACAGCAA GAGGAGACCA ACAATATGTG GTTCAATGCC 
ACACCATTTG AGAACGGAGC GCAATTCACT CTGATTGGTA TAATTATTGG CCTGGCGATC 
TATAACAACG TCACCCTGGC CGTAAACTTC CCCATGGTGG TCTATCGCAA GTTGATAGGC 
TATTGCGGCA CGTTCGCAGA CCTCAGCGAC TGGAGTCCGG CGCTGTACAA GAGTCTGAAG 
TCCATGCTGG ACTATCAGGG CCAGGATATG GAGGAGGTAT TCGAACAGAC CTTCAAGATT 
AGCTATAGCG ATGTTTTCGG TGATGTGGTA CAACACGAAC TGGTGCCGAA CGGCCAGGAT 
GTACTGGTGG GGCAGCACAA CAAGGAACTG TTTGTCAACC TGTACAGTGA TTTCCTGCTT 
AACACGAATA TCGAACAGCA GTTCAATGCT TTCCGCAAGG GCTTCGAAAT GGTTACGGAC 
GAATCGCCAC TGAAGCTGCT CTTCCGTCCA GAGGAGATTG AAATGCTGGT CTGCGGCAGT 
CGTGAATTCG ACTTTGTGGA ACTGGAAAAC TCCACTGTGT ACGAGGGCGG CTACACGGAG 
AAGAGCCAGT ACATCCAGGA CTTCTGGAGC ATAGTGCATG CGATGCCCAG CGAGGACAAG 
CACAAGCTGC TCGAATTCAC GACGGGATCG GCGCGTGTTC CGGTTGGCGG CCTGAAATGC 
CTGCGTCTGC TAATCACGCG TCACGGTCCG GATAGCGATC GGTTGCCTAC CTCGCACACC 
TGCTTCAACG TTCTCCTCCT GCCCGAGTAC AGCAGCCGCG AGAAGCTGGA GGAGCGCCTG 
ATGAAGGCCA TCAACTACTC GAAGGGCTTC GGCATGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648452.1
CDS	378..3299
Translation	MNGGGGGEDDQHLPGVSSASGSAAGAVGGRATPEMKRSAVRSLIKRYFHQLQSGCGNANCSNANCASSGKVAPMTPNEVAARALQLFSQDAQLCEAFTSEANSPQDVDMLSPNDSSSSSSGSSTSTITSASTTTTTSRQSQSTPAAVVVPVSSPYLTQSVPQLDIAGAEHSSGDSEPCTPTLAPVHSLDANSLIALYEQCRAADSYDRICHAIGVVFSSVDRLGKSFIRSAEPAASSSLQELLANSPGALNKEQLRTLEGEHDKDEDSTQQVDEQSESAANATATAESVESEAEPEEEDDDVCSSQSSDTLVDLPGLRRVQRLLFGCQIRAITEKLTSSVIQLSDWVIYMRTDWERVIHCLVICFDLATNTNNSVVDMDYLDRVLPKLCQAASAMPVPAQARLARIWAAHCSDQLHSLIAACQQQITLQVLLDEESMRENESIISVTKVLKIVFYANILASELERPSCRVPLEDRTEAATASGSAAVEDDLFVYNSLLEPHMPKFAEDQFEKELQVSAIDCRKPLIPLEEFYNEALSENIQMHHDYLSYKTLAMESEIGSGHANYFSFMLYAFILTPSTKVDALYYDSRMRMYSERYSSLHSILNNIGQVGQEDNPRPDLKLTVRRDQLINDALIGLEMVAMSNPKDLKKQLVVEFVGEQGIDEGGVSKEFFQLIVEEIFNPAFGMFIQQEETNNMWFNATPFENGAQFTLIGIIIGLAIYNNVTLAVNFPMVVYRKLIGYCGTFADLSDWSPALYKSLKSMLDYQGQDMEEVFEQTFKISYSDVFGDVVQHELVPNGQDVLVGQHNKELFVNLYSDFLLNTNIEQQFNAFRKGFEMVTDESPLKLLFRPEEIEMLVCGSREFDFVELENSTVYEGGYTEKSQYIQDFWSIVHAMPSEDKHKLLEFTTGSARVPVGGLKCLRLLITRHGPDSDRLPTSHTCFNVLLLPEYSSREKLEERLMKAINYSKGFGML

Target ORF information:

RefSeq Version	NM_140195.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin protein ligase E3A (Ube3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140195.4

ORF Insert Sequence:

ATGAACGGTG GCGGGGGTGG CGAGGATGAT CAGCATCTGC CAGGTGTTTC CAGTGCATCA 
GGATCTGCAG CTGGAGCGGT GGGCGGGCGA GCCACGCCCG AAATGAAGCG TTCGGCGGTG 
CGCAGCCTAA TCAAGCGATA CTTCCACCAA CTACAATCCG GCTGTGGGAA CGCCAACTGC 
AGCAATGCCA ATTGCGCCTC CAGCGGCAAG GTGGCGCCCA TGACGCCCAA CGAGGTGGCC 
GCCAGAGCAC TGCAGCTCTT TTCACAGGAT GCACAGCTCT GCGAGGCGTT CACATCGGAG 
GCCAACAGCC CCCAGGACGT GGACATGCTT TCGCCGAACG ACAGTAGCAG TAGTAGCAGC 
GGCAGCTCGA CAAGCACAAT TACCTCAGCC AGTACCACCA CGACAACCAG CAGACAATCG 
CAGTCAACGC CAGCTGCCGT TGTAGTGCCC GTATCCAGTC CATATCTTAC CCAGAGTGTG 
CCGCAACTGG ATATCGCCGG CGCTGAACAC TCCTCCGGCG ACTCGGAACC GTGTACACCC 
ACCCTGGCGC CAGTCCACAG CTTGGATGCC AACAGTCTGA TAGCGCTCTA TGAGCAATGT 
AGGGCGGCTG ACAGTTACGA CAGGATTTGC CACGCAATCG GCGTTGTGTT CTCCAGCGTA 
GATAGACTGG GCAAGAGCTT CATTCGCAGC GCAGAACCAG CGGCTTCTAG TAGTCTCCAG 
GAGCTGCTGG CCAATTCACC GGGGGCTTTA AACAAGGAGC AGCTACGAAC TTTGGAAGGG 
GAACACGATA AAGACGAAGA CAGTACACAA CAGGTGGACG AACAATCAGA ATCGGCGGCT 
AATGCTACTG CTACTGCAGA ATCAGTCGAA TCAGAGGCGG AGCCCGAAGA AGAGGACGAT 
GATGTGTGTT CATCCCAAAG CAGCGACACA CTGGTGGATC TGCCCGGTTT GCGACGAGTG 
CAGCGTCTCC TTTTTGGCTG TCAAATACGA GCTATAACGG AGAAGCTTAC CAGCAGCGTT 
ATTCAGCTGT CTGACTGGGT GATTTACATG CGTACGGATT GGGAGAGGGT CATTCACTGT 
CTGGTCATCT GCTTTGATCT AGCCACCAAT ACCAATAACA GTGTGGTGGA CATGGACTAT 
CTGGACAGGG TGCTGCCAAA ATTGTGCCAA GCGGCGTCGG CCATGCCAGT GCCGGCACAG 
GCGCGCCTGG CCAGGATCTG GGCTGCTCAT TGTTCAGATC AGCTGCATTC GTTGATCGCA 
GCCTGCCAGC AGCAAATTAC TCTACAGGTG CTTTTGGACG AGGAATCCAT GCGCGAGAAC 
GAATCCATAA TCAGTGTCAC CAAAGTTTTA AAGATTGTCT TCTACGCGAA CATTCTGGCT 
AGCGAGCTGG AGCGACCGTC GTGTCGGGTG CCGCTGGAGG ATCGAACGGA AGCGGCGACC 
GCATCTGGCA GCGCAGCAGT GGAGGACGAC CTATTCGTCT ACAACTCTTT GTTGGAGCCG 
CACATGCCCA AATTCGCCGA GGATCAGTTT GAAAAGGAGC TGCAGGTGTC GGCAATCGAT 
TGCCGGAAAC CACTGATACC ACTTGAGGAG TTCTACAACG AGGCACTCAG TGAAAACATT 
CAGATGCACC ACGACTACCT GTCCTACAAG ACGTTAGCCA TGGAGAGCGA GATTGGTTCC 
GGCCACGCGA ACTATTTTTC GTTCATGCTG TACGCCTTTA TCCTCACGCC GTCAACAAAA 
GTGGATGCCT TGTACTATGA CAGTCGAATG AGGATGTATA GCGAGCGCTA CTCCTCCCTT 
CACTCCATCC TCAACAATAT TGGCCAGGTA GGACAGGAAG ATAATCCTCG GCCGGATCTT 
AAGCTGACGG TGCGAAGGGA CCAGCTCATC AACGACGCAC TTATTGGGCT GGAAATGGTG 
GCCATGAGCA ATCCAAAGGA TCTCAAGAAG CAGTTGGTGG TGGAGTTTGT CGGCGAGCAG 
GGGATCGATG AAGGCGGCGT ATCCAAAGAG TTCTTCCAGC TAATCGTGGA GGAGATATTT 
AACCCGGCTT TTGGAATGTT TATACAGCAA GAGGAGACCA ACAATATGTG GTTCAATGCC 
ACACCATTTG AGAACGGAGC GCAATTCACT CTGATTGGTA TAATTATTGG CCTGGCGATC 
TATAACAACG TCACCCTGGC CGTAAACTTC CCCATGGTGG TCTATCGCAA GTTGATAGGC 
TATTGCGGCA CGTTCGCAGA CCTCAGCGAC TGGAGTCCGG CGCTGTACAA GAGTCTGAAG 
TCCATGCTGG ACTATCAGGG CCAGGATATG GAGGAGGTAT TCGAACAGAC CTTCAAGATT 
AGCTATAGCG ATGTTTTCGG TGATGTGGTA CAACACGAAC TGGTGCCGAA CGGCCAGGAT 
GTACTGGTGG GGCAGCACAA CAAGGAACTG TTTGTCAACC TGTACAGTGA TTTCCTGCTT 
AACACGAATA TCGAACAGCA GTTCAATGCT TTCCGCAAGG GCTTCGAAAT GGTTACGGAC 
GAATCGCCAC TGAAGCTGCT CTTCCGTCCA GAGGAGATTG AAATGCTGGT CTGCGGCAGT 
CGTGAATTCG ACTTTGTGGA ACTGGAAAAC TCCACTGTGT ACGAGGGCGG CTACACGGAG 
AAGAGCCAGT ACATCCAGGA CTTCTGGAGC ATAGTGCATG CGATGCCCAG CGAGGACAAG 
CACAAGCTGC TCGAATTCAC GACGGGATCG GCGCGTGTTC CGGTTGGCGG CCTGAAATGC 
CTGCGTCTGC TAATCACGCG TCACGGTCCG GATAGCGATC GGTTGCCTAC CTCGCACACC 
TGCTTCAACG TTCTCCTCCT GCCCGAGTAC AGCAGCCGCG AGAAGCTGGA GGAGCGCCTG 
ATGAAGGCCA TCAACTACTC GAAGGGCTTC GGCATGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ube3a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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