SCOT cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCOT
Entrez Gene ID	38261
Full Name	Succinyl-CoA:3-ketoacid CoA transferase
Synonyms	BcDNA:RH54340,CG1140,CT1116,DmSCOT,Dmel\CG1140,cDNA B
General protein information	Preferred Names Succinyl-CoA:3-ketoacid CoA transferase Names CG1140 gene product from transcript CG1140-RB 3-oxoacid CoA-transferase 3-oxoacid-CoA transferase 3-oxoacid-CoA-transferase CG1140-PA CG1140-PB CG1140-PC SCOT-PA SCOT-PB SCOT-PC citric acid synthetase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62B11-62B12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274372.1	NP_001261301.1	Succinyl-CoA:3-ketoacid CoA transferase, isoform C
NM_139426.3	NP_647683.1	Succinyl-CoA:3-ketoacid CoA transferase, isoform A
NM_167928.2	NP_728699.1	Succinyl-CoA:3-ketoacid CoA transferase, isoform B

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	OXCT1	XP_001114587.1
Gallus gallus (chicken)	OXCT1	NP_001006578.2
Caenorhabditis elegans (roundworm)	C05C10.3	NP_496144.1
Xenopus tropicalis (tropical clawed frog)	oxct1	NP_001007927.1
Neurospora crassa	NCU06881	XP_959228.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006096	XP_316153.4
Canis lupus familiaris (dog)	OXCT1	XP_536487.2
Rattus norvegicus (Norway rat)	Oxct1	NP_001121052.1
Magnaporthe oryzae (rice blast fungus)	MGG_11480	XP_003718114.1
Pan troglodytes (chimpanzee)	OXCT1	XP_001137948.1
Homo sapiens (human)	OXCT1	NP_000427.1
Mus musculus (house mouse)	Oxct1	NP_077150.1
Danio rerio (zebrafish)	oxct1b	NP_001070618.1
Drosophila melanogaster (fruit fly)	CG1140	NP_647683.1
Bos taurus (cattle)	OXCT1	NP_001069538.1
Danio rerio (zebrafish)	oxct1a	NP_001007292.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SCOT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCOT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa20447	NM_001274372.1 Latest version!	Drosophila melanogaster Succinyl-CoA:3-ketoacid CoA transferase (SCOT), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa17257	NM_167928.2 Latest version!	Drosophila melanogaster Succinyl-CoA:3-ketoacid CoA transferase (SCOT), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa17258	NM_139426.3 Latest version!	Drosophila melanogaster Succinyl-CoA:3-ketoacid CoA transferase (SCOT), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCOT ( NM_001274372.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261301.1 Drosophila melanogaster Succinyl-CoA:3-ketoacid CoA transferase (SCOT), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa20447
Clone ID Related Accession (Same CDS sequence)	NM_001274372.1
Accession Version	NM_001274372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Succinyl-CoA:3-ketoacid CoA transferase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCAACG CGCTCAAGCA GAAAGGAGTC AAGAACATCA CTGGCGTTTC CAACAATGGA 
GGTGTGGACG ACACTGGTCT GGGTGTCCTG ATCAAGCAGA AGCAGGTGTC CAAGGTAATT 
GGCTCCTATG TCGGCGAAAA CACGGAGTTG GTGCGTCAAT ATCTGGAAGG AGAACTGGCC 
GTGGAACTGA CTCCCCAAGG CACCCTAGCG GAAAAGATCC GCGCTGGAGG AGCCGGCATT 
CCGGCCTTCT ACACGCCCAC TGGCTACGCC ACTCTTGTCC AGGAAGGCGG AGCACCCATT 
AAGTACTCCA AGGATGGCAA AGTGGAAATA TCCAGCGAAA AGAAGCCCGT GAAGGAGTTC 
AACGGAAAGA ACTACGTGAT GGAGGAGTCC ATCTTCGCAG ACTTTGCCTT TGTCAAGGCC 
CAGAAGGCCG ATCCCTTGGG CAATCTGGTG TTCAACAAAG CTGCCAGAAA CTTCAATGCA 
CCCATGTGCA GGGCTGCCAA AATCACCGTG GCCGAAGTGG AGGAAATCGT GCCCATCGGA 
GCCCTTTCTC CCGATGAGAT CCATGTGCCC GGCATCTACA TTAACCGCAT CTTCAAGGGC 
ACCAACTACA ACAAGCGTGT GGAACGCTTG CGCATCACGG AACCCAAGGA TCCCAGCAAA 
CCCGCTCCTC CACCAAATCC CGCCCAGGTT TTACGTGAGC GCATTGCCCG TCGAGTGGCT 
CTTGAGTTCC ACGACGGCAT GTATGCCAAT CTGGGCATTG GCATTCCCGT GCTCTCCTCC 
AACTACATCC CCAAGGGCAT GAACGTGATG CTGCAGTCGG AGAACGGAAT CCTGGGCCTG 
GGACCCTTCC CCACCAAGGA TAAAGTGGAT CCGGACTTGA TCAACGCCGG AAAGGAGAGT 
GTTACCGTGG TGCCCGGAGC CTCATACTTT GGCTCCGATG ACAGCTTTGC CATGATCCGT 
GGTGGTCATG TGGATATCAC CATTTTGGGC GCCATGGAGG TGTCGGCCAC AGGCGATCTG 
GCCAACTGGA TGATTCCTGG CAAACTGGTT AAGGGAATGG GTGGAGCCAT GGATTTGGTG 
GCTGCGCCTG GCACCAAGGT GATCATCACC ATGGAGCACA ATGCCCGCGA TGGTTCCCCC 
AAGATCCTGG ACACCTGCTC CCTTCCGCTC ACTGGCAAGG GTGTGATCGA CATGATCATC 
TCGGAGAAGG CTGTCTTCAC GGTGGAAAAG GGCGTGGGCC TAACGCTGAT CGAGGTGGCC 
GAGGGTTACA CCGTGGACGA CATCATTGCC AGCACTGGAG CCAAGTTCAC AGTGTCGCCC 
AATCTGAAGA AAATGGGTCA AATTCCGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261301.1
CDS	528..1880
Translation	MINALKQKGVKNITGVSNNGGVDDTGLGVLIKQKQVSKVIGSYVGENTELVRQYLEGELAVELTPQGTLAEKIRAGGAGIPAFYTPTGYATLVQEGGAPIKYSKDGKVEISSEKKPVKEFNGKNYVMEESIFADFAFVKAQKADPLGNLVFNKAARNFNAPMCRAAKITVAEVEEIVPIGALSPDEIHVPGIYINRIFKGTNYNKRVERLRITEPKDPSKPAPPPNPAQVLRERIARRVALEFHDGMYANLGIGIPVLSSNYIPKGMNVMLQSENGILGLGPFPTKDKVDPDLINAGKESVTVVPGASYFGSDDSFAMIRGGHVDITILGAMEVSATGDLANWMIPGKLVKGMGGAMDLVAAPGTKVIITMEHNARDGSPKILDTCSLPLTGKGVIDMIISEKAVFTVEKGVGLTLIEVAEGYTVDDIIASTGAKFTVSPNLKKMGQIPV

Target ORF information:

RefSeq Version	NM_001274372.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Succinyl-CoA:3-ketoacid CoA transferase (SCOT), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274372.1

ORF Insert Sequence:

ATGATCAACG CGCTCAAGCA GAAAGGAGTC AAGAACATCA CTGGCGTTTC CAACAATGGA 
GGTGTGGACG ACACTGGTCT GGGTGTCCTG ATCAAGCAGA AGCAGGTGTC CAAGGTAATT 
GGCTCCTATG TCGGCGAAAA CACGGAGTTG GTGCGTCAAT ATCTGGAAGG AGAACTGGCC 
GTGGAACTGA CTCCCCAAGG CACCCTAGCG GAAAAGATCC GCGCTGGAGG AGCCGGCATT 
CCGGCCTTCT ACACGCCCAC TGGCTACGCC ACTCTTGTCC AGGAAGGCGG AGCACCCATT 
AAGTACTCCA AGGATGGCAA AGTGGAAATA TCCAGCGAAA AGAAGCCCGT GAAGGAGTTC 
AACGGAAAGA ACTACGTGAT GGAGGAGTCC ATCTTCGCAG ACTTTGCCTT TGTCAAGGCC 
CAGAAGGCCG ATCCCTTGGG CAATCTGGTG TTCAACAAAG CTGCCAGAAA CTTCAATGCA 
CCCATGTGCA GGGCTGCCAA AATCACCGTG GCCGAAGTGG AGGAAATCGT GCCCATCGGA 
GCCCTTTCTC CCGATGAGAT CCATGTGCCC GGCATCTACA TTAACCGCAT CTTCAAGGGC 
ACCAACTACA ACAAGCGTGT GGAACGCTTG CGCATCACGG AACCCAAGGA TCCCAGCAAA 
CCCGCTCCTC CACCAAATCC CGCCCAGGTT TTACGTGAGC GCATTGCCCG TCGAGTGGCT 
CTTGAGTTCC ACGACGGCAT GTATGCCAAT CTGGGCATTG GCATTCCCGT GCTCTCCTCC 
AACTACATCC CCAAGGGCAT GAACGTGATG CTGCAGTCGG AGAACGGAAT CCTGGGCCTG 
GGACCCTTCC CCACCAAGGA TAAAGTGGAT CCGGACTTGA TCAACGCCGG AAAGGAGAGT 
GTTACCGTGG TGCCCGGAGC CTCATACTTT GGCTCCGATG ACAGCTTTGC CATGATCCGT 
GGTGGTCATG TGGATATCAC CATTTTGGGC GCCATGGAGG TGTCGGCCAC AGGCGATCTG 
GCCAACTGGA TGATTCCTGG CAAACTGGTT AAGGGAATGG GTGGAGCCAT GGATTTGGTG 
GCTGCGCCTG GCACCAAGGT GATCATCACC ATGGAGCACA ATGCCCGCGA TGGTTCCCCC 
AAGATCCTGG ACACCTGCTC CCTTCCGCTC ACTGGCAAGG GTGTGATCGA CATGATCATC 
TCGGAGAAGG CTGTCTTCAC GGTGGAAAAG GGCGTGGGCC TAACGCTGAT CGAGGTGGCC 
GAGGGTTACA CCGTGGACGA CATCATTGCC AGCACTGGAG CCAAGTTCAC AGTGTCGCCC 
AATCTGAAGA AAATGGGTCA AATTCCGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCOT ( NM_167928.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728699.1 Drosophila melanogaster Succinyl-CoA:3-ketoacid CoA transferase (SCOT), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17257
Clone ID Related Accession (Same CDS sequence)	NM_167928.2
Accession Version	NM_167928.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Succinyl-CoA:3-ketoacid CoA transferase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167928.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGGCT GGCTTGCGGC TATTGCGTGC TACTCCACGT CGGGAAAACA GCGGAACGGC 
AAGATCTACG AGTCGGCCAT AGATGCTGTG GCCGATGTGC AGGATGGTGC CCAGATCCTG 
TTCGGCGGAT TCGGAATCTG CGGCATTCCG GAGAAGATGA TCAACGCGCT CAAGCAGAAA 
GGAGTCAAGA ACATCACTGG CGTTTCCAAC AATGGAGGTG TGGACGACAC TGGTCTGGGT 
GTCCTGATCA AGCAGAAGCA GGTGTCCAAG GTAATTGGCT CCTATGTCGG CGAAAACACG 
GAGTTGGTGC GTCAATATCT GGAAGGAGAA CTGGCCGTGG AACTGACTCC CCAAGGCACC 
CTAGCGGAAA AGATCCGCGC TGGAGGAGCC GGCATTCCGG CCTTCTACAC GCCCACTGGC 
TACGCCACTC TTGTCCAGGA AGGCGGAGCA CCCATTAAGT ACTCCAAGGA TGGCAAAGTG 
GAAATATCCA GCGAAAAGAA GCCCGTGAAG GAGTTCAACG GAAAGAACTA CGTGATGGAG 
GAGTCCATCT TCGCAGACTT TGCCTTTGTC AAGGCCCAGA AGGCCGATCC CTTGGGCAAT 
CTGGTGTTCA ACAAAGCTGC CAGAAACTTC AATGCACCCA TGTGCAGGGC TGCCAAAATC 
ACCGTGGCCG AAGTGGAGGA AATCGTGCCC ATCGGAGCCC TTTCTCCCGA TGAGATCCAT 
GTGCCCGGCA TCTACATTAA CCGCATCTTC AAGGGCACCA ACTACAACAA GCGTGTGGAA 
CGCTTGCGCA TCACGGAACC CAAGGATCCC AGCAAACCCG CTCCTCCACC AAATCCCGCC 
CAGGTTTTAC GTGAGCGCAT TGCCCGTCGA GTGGCTCTTG AGTTCCACGA CGGCATGTAT 
GCCAATCTGG GCATTGGCAT TCCCGTGCTC TCCTCCAACT ACATCCCCAA GGGCATGAAC 
GTGATGCTGC AGTCGGAGAA CGGAATCCTG GGCCTGGGAC CCTTCCCCAC CAAGGATAAA 
GTGGATCCGG ACTTGATCAA CGCCGGAAAG GAGAGTGTTA CCGTGGTGCC CGGAGCCTCA 
TACTTTGGCT CCGATGACAG CTTTGCCATG ATCCGTGGTG GTCATGTGGA TATCACCATT 
TTGGGCGCCA TGGAGGTGTC GGCCACAGGC GATCTGGCCA ACTGGATGAT TCCTGGCAAA 
CTGGTTAAGG GAATGGGTGG AGCCATGGAT TTGGTGGCTG CGCCTGGCAC CAAGGTGATC 
ATCACCATGG AGCACAATGC CCGCGATGGT TCCCCCAAGA TCCTGGACAC CTGCTCCCTT 
CCGCTCACTG GCAAGGGTGT GATCGACATG ATCATCTCGG AGAAGGCTGT CTTCACGGTG 
GAAAAGGGCG TGGGCCTAAC GCTGATCGAG GTGGCCGAGG GTTACACCGT GGACGACATC 
ATTGCCAGCA CTGGAGCCAA GTTCACAGTG TCGCCCAATC TGAAGAAAAT GGGTCAAATT 
CCGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728699.1
CDS	332..1840
Translation	MDGWLAAIACYSTSGKQRNGKIYESAIDAVADVQDGAQILFGGFGICGIPEKMINALKQKGVKNITGVSNNGGVDDTGLGVLIKQKQVSKVIGSYVGENTELVRQYLEGELAVELTPQGTLAEKIRAGGAGIPAFYTPTGYATLVQEGGAPIKYSKDGKVEISSEKKPVKEFNGKNYVMEESIFADFAFVKAQKADPLGNLVFNKAARNFNAPMCRAAKITVAEVEEIVPIGALSPDEIHVPGIYINRIFKGTNYNKRVERLRITEPKDPSKPAPPPNPAQVLRERIARRVALEFHDGMYANLGIGIPVLSSNYIPKGMNVMLQSENGILGLGPFPTKDKVDPDLINAGKESVTVVPGASYFGSDDSFAMIRGGHVDITILGAMEVSATGDLANWMIPGKLVKGMGGAMDLVAAPGTKVIITMEHNARDGSPKILDTCSLPLTGKGVIDMIISEKAVFTVEKGVGLTLIEVAEGYTVDDIIASTGAKFTVSPNLKKMGQIPV

Target ORF information:

RefSeq Version	NM_167928.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Succinyl-CoA:3-ketoacid CoA transferase (SCOT), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167928.2

ORF Insert Sequence:

ATGGATGGCT GGCTTGCGGC TATTGCGTGC TACTCCACGT CGGGAAAACA GCGGAACGGC 
AAGATCTACG AGTCGGCCAT AGATGCTGTG GCCGATGTGC AGGATGGTGC CCAGATCCTG 
TTCGGCGGAT TCGGAATCTG CGGCATTCCG GAGAAGATGA TCAACGCGCT CAAGCAGAAA 
GGAGTCAAGA ACATCACTGG CGTTTCCAAC AATGGAGGTG TGGACGACAC TGGTCTGGGT 
GTCCTGATCA AGCAGAAGCA GGTGTCCAAG GTAATTGGCT CCTATGTCGG CGAAAACACG 
GAGTTGGTGC GTCAATATCT GGAAGGAGAA CTGGCCGTGG AACTGACTCC CCAAGGCACC 
CTAGCGGAAA AGATCCGCGC TGGAGGAGCC GGCATTCCGG CCTTCTACAC GCCCACTGGC 
TACGCCACTC TTGTCCAGGA AGGCGGAGCA CCCATTAAGT ACTCCAAGGA TGGCAAAGTG 
GAAATATCCA GCGAAAAGAA GCCCGTGAAG GAGTTCAACG GAAAGAACTA CGTGATGGAG 
GAGTCCATCT TCGCAGACTT TGCCTTTGTC AAGGCCCAGA AGGCCGATCC CTTGGGCAAT 
CTGGTGTTCA ACAAAGCTGC CAGAAACTTC AATGCACCCA TGTGCAGGGC TGCCAAAATC 
ACCGTGGCCG AAGTGGAGGA AATCGTGCCC ATCGGAGCCC TTTCTCCCGA TGAGATCCAT 
GTGCCCGGCA TCTACATTAA CCGCATCTTC AAGGGCACCA ACTACAACAA GCGTGTGGAA 
CGCTTGCGCA TCACGGAACC CAAGGATCCC AGCAAACCCG CTCCTCCACC AAATCCCGCC 
CAGGTTTTAC GTGAGCGCAT TGCCCGTCGA GTGGCTCTTG AGTTCCACGA CGGCATGTAT 
GCCAATCTGG GCATTGGCAT TCCCGTGCTC TCCTCCAACT ACATCCCCAA GGGCATGAAC 
GTGATGCTGC AGTCGGAGAA CGGAATCCTG GGCCTGGGAC CCTTCCCCAC CAAGGATAAA 
GTGGATCCGG ACTTGATCAA CGCCGGAAAG GAGAGTGTTA CCGTGGTGCC CGGAGCCTCA 
TACTTTGGCT CCGATGACAG CTTTGCCATG ATCCGTGGTG GTCATGTGGA TATCACCATT 
TTGGGCGCCA TGGAGGTGTC GGCCACAGGC GATCTGGCCA ACTGGATGAT TCCTGGCAAA 
CTGGTTAAGG GAATGGGTGG AGCCATGGAT TTGGTGGCTG CGCCTGGCAC CAAGGTGATC 
ATCACCATGG AGCACAATGC CCGCGATGGT TCCCCCAAGA TCCTGGACAC CTGCTCCCTT 
CCGCTCACTG GCAAGGGTGT GATCGACATG ATCATCTCGG AGAAGGCTGT CTTCACGGTG 
GAAAAGGGCG TGGGCCTAAC GCTGATCGAG GTGGCCGAGG GTTACACCGT GGACGACATC 
ATTGCCAGCA CTGGAGCCAA GTTCACAGTG TCGCCCAATC TGAAGAAAAT GGGTCAAATT 
CCGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCOT ( NM_139426.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647683.1 Drosophila melanogaster Succinyl-CoA:3-ketoacid CoA transferase (SCOT), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17258
Clone ID Related Accession (Same CDS sequence)	NM_139426.3
Accession Version	NM_139426.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Succinyl-CoA:3-ketoacid CoA transferase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139426.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCTGCC GACTGGTGGG AAACAGGAGT TTGGGTGCAC GATATACCGC CTCCATCAAG 
GCTATTGCGT GCTACTCCAC GTCGGGAAAA CAGCGGAACG GCAAGATCTA CGAGTCGGCC 
ATAGATGCTG TGGCCGATGT GCAGGATGGT GCCCAGATCC TGTTCGGCGG ATTCGGAATC 
TGCGGCATTC CGGAGAAGAT GATCAACGCG CTCAAGCAGA AAGGAGTCAA GAACATCACT 
GGCGTTTCCA ACAATGGAGG TGTGGACGAC ACTGGTCTGG GTGTCCTGAT CAAGCAGAAG 
CAGGTGTCCA AGGTAATTGG CTCCTATGTC GGCGAAAACA CGGAGTTGGT GCGTCAATAT 
CTGGAAGGAG AACTGGCCGT GGAACTGACT CCCCAAGGCA CCCTAGCGGA AAAGATCCGC 
GCTGGAGGAG CCGGCATTCC GGCCTTCTAC ACGCCCACTG GCTACGCCAC TCTTGTCCAG 
GAAGGCGGAG CACCCATTAA GTACTCCAAG GATGGCAAAG TGGAAATATC CAGCGAAAAG 
AAGCCCGTGA AGGAGTTCAA CGGAAAGAAC TACGTGATGG AGGAGTCCAT CTTCGCAGAC 
TTTGCCTTTG TCAAGGCCCA GAAGGCCGAT CCCTTGGGCA ATCTGGTGTT CAACAAAGCT 
GCCAGAAACT TCAATGCACC CATGTGCAGG GCTGCCAAAA TCACCGTGGC CGAAGTGGAG 
GAAATCGTGC CCATCGGAGC CCTTTCTCCC GATGAGATCC ATGTGCCCGG CATCTACATT 
AACCGCATCT TCAAGGGCAC CAACTACAAC AAGCGTGTGG AACGCTTGCG CATCACGGAA 
CCCAAGGATC CCAGCAAACC CGCTCCTCCA CCAAATCCCG CCCAGGTTTT ACGTGAGCGC 
ATTGCCCGTC GAGTGGCTCT TGAGTTCCAC GACGGCATGT ATGCCAATCT GGGCATTGGC 
ATTCCCGTGC TCTCCTCCAA CTACATCCCC AAGGGCATGA ACGTGATGCT GCAGTCGGAG 
AACGGAATCC TGGGCCTGGG ACCCTTCCCC ACCAAGGATA AAGTGGATCC GGACTTGATC 
AACGCCGGAA AGGAGAGTGT TACCGTGGTG CCCGGAGCCT CATACTTTGG CTCCGATGAC 
AGCTTTGCCA TGATCCGTGG TGGTCATGTG GATATCACCA TTTTGGGCGC CATGGAGGTG 
TCGGCCACAG GCGATCTGGC CAACTGGATG ATTCCTGGCA AACTGGTTAA GGGAATGGGT 
GGAGCCATGG ATTTGGTGGC TGCGCCTGGC ACCAAGGTGA TCATCACCAT GGAGCACAAT 
GCCCGCGATG GTTCCCCCAA GATCCTGGAC ACCTGCTCCC TTCCGCTCAC TGGCAAGGGT 
GTGATCGACA TGATCATCTC GGAGAAGGCT GTCTTCACGG TGGAAAAGGG CGTGGGCCTA 
ACGCTGATCG AGGTGGCCGA GGGTTACACC GTGGACGACA TCATTGCCAG CACTGGAGCC 
AAGTTCACAG TGTCGCCCAA TCTGAAGAAA ATGGGTCAAA TTCCGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647683.1
CDS	92..1642
Translation	MLCRLVGNRSLGARYTASIKAIACYSTSGKQRNGKIYESAIDAVADVQDGAQILFGGFGICGIPEKMINALKQKGVKNITGVSNNGGVDDTGLGVLIKQKQVSKVIGSYVGENTELVRQYLEGELAVELTPQGTLAEKIRAGGAGIPAFYTPTGYATLVQEGGAPIKYSKDGKVEISSEKKPVKEFNGKNYVMEESIFADFAFVKAQKADPLGNLVFNKAARNFNAPMCRAAKITVAEVEEIVPIGALSPDEIHVPGIYINRIFKGTNYNKRVERLRITEPKDPSKPAPPPNPAQVLRERIARRVALEFHDGMYANLGIGIPVLSSNYIPKGMNVMLQSENGILGLGPFPTKDKVDPDLINAGKESVTVVPGASYFGSDDSFAMIRGGHVDITILGAMEVSATGDLANWMIPGKLVKGMGGAMDLVAAPGTKVIITMEHNARDGSPKILDTCSLPLTGKGVIDMIISEKAVFTVEKGVGLTLIEVAEGYTVDDIIASTGAKFTVSPNLKKMGQIPV

Target ORF information:

RefSeq Version	NM_139426.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Succinyl-CoA:3-ketoacid CoA transferase (SCOT), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139426.3

ORF Insert Sequence:

ATGCTCTGCC GACTGGTGGG AAACAGGAGT TTGGGTGCAC GATATACCGC CTCCATCAAG 
GCTATTGCGT GCTACTCCAC GTCGGGAAAA CAGCGGAACG GCAAGATCTA CGAGTCGGCC 
ATAGATGCTG TGGCCGATGT GCAGGATGGT GCCCAGATCC TGTTCGGCGG ATTCGGAATC 
TGCGGCATTC CGGAGAAGAT GATCAACGCG CTCAAGCAGA AAGGAGTCAA GAACATCACT 
GGCGTTTCCA ACAATGGAGG TGTGGACGAC ACTGGTCTGG GTGTCCTGAT CAAGCAGAAG 
CAGGTGTCCA AGGTAATTGG CTCCTATGTC GGCGAAAACA CGGAGTTGGT GCGTCAATAT 
CTGGAAGGAG AACTGGCCGT GGAACTGACT CCCCAAGGCA CCCTAGCGGA AAAGATCCGC 
GCTGGAGGAG CCGGCATTCC GGCCTTCTAC ACGCCCACTG GCTACGCCAC TCTTGTCCAG 
GAAGGCGGAG CACCCATTAA GTACTCCAAG GATGGCAAAG TGGAAATATC CAGCGAAAAG 
AAGCCCGTGA AGGAGTTCAA CGGAAAGAAC TACGTGATGG AGGAGTCCAT CTTCGCAGAC 
TTTGCCTTTG TCAAGGCCCA GAAGGCCGAT CCCTTGGGCA ATCTGGTGTT CAACAAAGCT 
GCCAGAAACT TCAATGCACC CATGTGCAGG GCTGCCAAAA TCACCGTGGC CGAAGTGGAG 
GAAATCGTGC CCATCGGAGC CCTTTCTCCC GATGAGATCC ATGTGCCCGG CATCTACATT 
AACCGCATCT TCAAGGGCAC CAACTACAAC AAGCGTGTGG AACGCTTGCG CATCACGGAA 
CCCAAGGATC CCAGCAAACC CGCTCCTCCA CCAAATCCCG CCCAGGTTTT ACGTGAGCGC 
ATTGCCCGTC GAGTGGCTCT TGAGTTCCAC GACGGCATGT ATGCCAATCT GGGCATTGGC 
ATTCCCGTGC TCTCCTCCAA CTACATCCCC AAGGGCATGA ACGTGATGCT GCAGTCGGAG 
AACGGAATCC TGGGCCTGGG ACCCTTCCCC ACCAAGGATA AAGTGGATCC GGACTTGATC 
AACGCCGGAA AGGAGAGTGT TACCGTGGTG CCCGGAGCCT CATACTTTGG CTCCGATGAC 
AGCTTTGCCA TGATCCGTGG TGGTCATGTG GATATCACCA TTTTGGGCGC CATGGAGGTG 
TCGGCCACAG GCGATCTGGC CAACTGGATG ATTCCTGGCA AACTGGTTAA GGGAATGGGT 
GGAGCCATGG ATTTGGTGGC TGCGCCTGGC ACCAAGGTGA TCATCACCAT GGAGCACAAT 
GCCCGCGATG GTTCCCCCAA GATCCTGGAC ACCTGCTCCC TTCCGCTCAC TGGCAAGGGT 
GTGATCGACA TGATCATCTC GGAGAAGGCT GTCTTCACGG TGGAAAAGGG CGTGGGCCTA 
ACGCTGATCG AGGTGGCCGA GGGTTACACC GTGGACGACA TCATTGCCAG CACTGGAGCC 
AAGTTCACAG TGTCGCCCAA TCTGAAGAAA ATGGGTCAAA TTCCGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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SCOT cDNA ORF clone, Drosophila melanogaster(fruit fly)

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ORF Insert Sequence:

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ORF Insert Sequence:

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ORF Insert Sequence:

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