Top3alpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Top3alpha
Entrez Gene ID	35236
Full Name	Topoisomerase 3alpha
Synonyms	CG10123,DmTop3alpha,Dmel\CG10123,Top3a,Topo IIIalpha,dTopoIIIa,top3alpha,topo IIIalpha
General protein information	Preferred Names Topoisomerase 3alpha Names CG10123 gene product from transcript CG10123-RA CG10123-PA Top 3alpha Top3alpha-PA Topoisomerase3alpha topoisomerase 3-alpha topoisomerase 3alpha topoisomerase IIIa topoisomerase IIIalpha
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37E4-37E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_078878.3	NP_523602.2	topoisomerase 3alpha

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	top3a	XP_688695.4
Pan troglodytes (chimpanzee)	TOP3A	XP_511323.2
Macaca mulatta (Rhesus monkey)	TOP3A	XP_001095437.2
Caenorhabditis elegans (roundworm)	top-3	NP_499558.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010028	XP_319175.3
Xenopus tropicalis (tropical clawed frog)	top3a	XP_002935889.1
Homo sapiens (human)	TOP3A	NP_004609.1
Bos taurus (cattle)	TOP3A	NP_001071443.1
Mus musculus (house mouse)	Top3a	NP_033436.1
Rattus norvegicus (Norway rat)	Top3a	XP_006246613.1
Gallus gallus (chicken)	TOP3A	NP_001025807.1
Arabidopsis thaliana (thale cress)	TOP3A	NP_201197.1
Canis lupus familiaris (dog)	TOP3A	XP_546656.2
Drosophila melanogaster (fruit fly)	Top3alpha	NP_523602.2
Oryza sativa (rice)	Os03g0165000	NP_001173278.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Top3alpha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top3alpha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14980	NM_078878.3 Latest version!	Drosophila melanogaster topoisomerase 3alpha (Top3alpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Top3alpha ( NM_078878.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523602.2 Drosophila melanogaster topoisomerase 3alpha (Top3alpha), mRNA.

Price & Availability↑

CloneID	OFa14980
Clone ID Related Accession (Same CDS sequence)	NM_078878.3
Accession Version	NM_078878.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase 3alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078878.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAGCTG TCTTAACATG TTTTCGCCCC GTCGTCGCCT CACACAGAAG ATATTGCGCT 
AATCCCGGCC AGGCAATGAA GTACTTAAAC GTGGCTGAGA AGAACGACGC CGCCAAAACG 
ATTGCCGGCC TGCTGTCAAA TGGCGCAGCA CAAAGGCGCG AAGGCTACTC GGTGTACAAC 
AAGGTCTTCG ATTTCGAGGC CCCTGTGCGC GGTCAGAATG CCAAAATGGT TATGACCTCC 
GTATCCGGCC ACATGATGCA GCTGGCATTC CAGGTGTCTT ACAAAAATTG GAGAACGGTG 
GATCCGCGCT CTTTATTCGA CGCGCCGGTG AAAAAGGGCG TGGGCTCGGA TTATGAGCCT 
ATCAAAAGGA CCTTGGAACG CGAGGTGCGC GGCTGTCAGG GATTGATTAT CTGGACGGAT 
TGCGATCGCG AGGGCGAGAA CATTGGCTAC GAGATTATTG ATGTGTGTCG CGCTATCAAA 
CCGAATATTT CGGTTTATCG TGCCACTTTC TCGGAGATTA CCACGGTGGC GGTGCGTCGG 
GCTCTGCAGC AATTGGGGCA GCCGGACAAA AGGCAGAGCG ATGCAGTGGA TGTGCGCACG 
GAACTGGATT TGCGAACTGG TGCCGCCATC ACCCGGTTTC AGACAATGCG CCTACAACGT 
CTGTTCCCCG AAAAGATAGC TGATAAGCTC ATATCCTATG GAAGCTGCCA AATTCCCACC 
CTGGGATTCG TCGCCGAACG GTACAAGGAG ATTGAGGCCT TCGTATCCGA GCCATTTTGG 
AAGATTAAGG TTCTGCACAC AATTGATGAC TTGACGGTGG AGTTTAATTG GGCCCGAAAC 
CGACTCTTCG ATAAGGAAGC TTGTGAAAAC TATTTGCTCC TCTGCCTGGC CGAACCCGAT 
CCTAGAGCGC TCGTGGAGAG CGTCACCGTT AAGCCCAAAC ACAAATGGCG CCCAACTCCG 
CTGGATACCG TGGAAATGGA GAAGCTTGGC TCGAGGAAAC TCAAGCTATC TGCCAAGGAG 
ACGATGACAA TAGCCGAAAA ATTATATACC AAGGGTTTCA TCAGCTATCC CCGTACGGAA 
ACCAACCAGT TCTCCAAGGA GTTCGCTCTG GCACCACTAG TTGAGATGCA AACGGGTCAC 
CGCGACTGGG GAGCATTTGC CCAGCGGGTG ATTGAGTGGG GACCAAATCC TCGAAACGGT 
AACAAATCGG ATCAGGCCCA TCCTCCAATT CATCCCACCA AGCTGGCAGA AAACCTGCAG 
GGAAACGAGG CGCGAGTGTA CGAACTGGTC GTCCGGCACT TCCTCGCCTG CGTCAGCAAA 
GATGCAGTCG GCTCCGAAAC CCTTGTCCAT ATCGACATTG CCGGTGAGAA GTTCACGGCC 
AATGGTCTGG TTATTCACGA ACGAAACTAC CTAGATGTTT ATGTTTATGA CAAGTGGAGT 
GCCAAGCAGA TCCATCACTA TGAAAACGGG CAGCGTTTTG AGCCCACGGA AGTGTCGCTC 
CACGAAGGTG CCACCACTGC CCCGCCCCTG CTGACCGAAG CGGATTTGAT TGCACTGATG 
GAAAAGCATG GGATTGGTAC AGATGCCACG CACGCTGAGC ACATAAACAC GATCAAGGAA 
CGCGGATACA TCGGAGTGCT GGATAAGGGC TTTTTGGTAC CTGGAGTTAT TGGAATGGGA 
CTGTATGAGG GATACGATGC CATGGAGCTG GCCCTTGCCA AGCCACAACT TCGTGCTGAG 
TTTGAATTGG ATCTGAAACT AATTTGCCAG GGGCAGAAAG ATCCCAAGGT GGTCCTGACC 
GAACAGATAG CCAAGTACAA GCAGGCCTAT CAGCAGATTA CCGATAAGAT TACCGCCATG 
GACGCCAAGA TATCGGCTCG CATTAATGAG ACACCAGCTG CCAATTCTGC GGTGCAGGAA 
GGAGCTGATG GAAGTGCTCC AAGTCACGGC ATCATCCAAT CGATCTTCCA GTGCCCTAAG 
TGCAATGAGG CTCCTCTGGC CCTAAAGCCA AAGAAGAACC AGCAGGGCTG GTACATTGGC 
TGCAATAACT TTCCCGACTG CAAAAACGCA GTTTGGCTTC CAACGGAGTG CAAGGACGCA 
TCTGTGCTGG ACGAGTGCTG TCCCACCTGT GGCGATGGAT ACCGGATGCT CAAGTTCCGC 
CTGAGCACGC CTTACTATCG CGGGGTATTT GGTACTCCCA GCGGTTGGTA CAAAACCTGC 
TTGCCCTGCG ATAATCTCTT TCGCACCACG TTCAACATTA ATTTGGACTC GGTTAAGAAA 
GTGGGAGGCA TTGTGGGTGA AGTGCGTGGT GGAGGAGGGG GTCCAGGCCC AGGACCCGGT 
GGCGGTGGCT CTGGCAGAGG AGCCGGCTCT GGTGGATGGA GCAGCGGACC TGGAGGCGGT 
GGCTCTGGCG GAGGCGGCGG CTCTGGAGGA TGGGGCAGCG GAACTGGAGG CGGTGGCTCT 
GGAGGATGGG GCAGCGGAAC TGGAGGCGGT GGCTTGGGCG GAGGAAAGGG CAAGAAACCT 
GGCGGCGAAT CTAAAAAATC AGCTACAAAA AAACCTCCGA ACGAACCCAA ACCGAAAAAG 
ACGAAAGAAC CGAAAGCGGC ACCCAACAAA AAGACTAGCA GCAAATCGTC TGGCAGCATA 
CGGAGCTTCT TTACAAGTGC GGCGCCTACA AATTCCGCCA GCAATGGACT AGATGAGTTT 
TTCGATAGTA ACGATGGCTT TGAGGACGCC ATGTTGGCTG CTGCCGAATC CGTGGAATCG 
AGTAGCCAAC CCAAAACCAT TAGTATGGTC CCTCTTGATG ACGACATAGC CGCCGCTTTT 
GCAGCGGATG ATGATGCTGA ATTCGAGGCG TTGGTAAACG GCGGAACCAT GCCCACCGAA 
TCCAATGGGG ACCAGCAGCT GGACAAAAGT CTCTCGGAAT GGATAAAGGA GCAGGACAAA 
GCGGACGAGA GGCCCATGCT TTGGGGAACT CGGGAGCGAG CGTCGTTAGG TACTGCTGCT 
CCCACTCCTC CTCCGAAACC GGCAGCTAAA CGACCGAGAT GGGACAGTGT CGAACGGGAT 
TCCACACCAC CCTCTTCGGT TCCGGAATCC GAAACAGTGC TGTGTACCGG ATGTCAGCAG 
CCAGCTCGCC AGAACACTGT CCGTAAGAAT GGACCCAACC TAGGCAGGCT GTACTACAAG 
TGTCCCAAAC CGGATGAGTG CAACTTCTTT CAGTGGGCTG ATGAGCCACC GTCGTCGGCC 
AAAAGCAAGA ATTCCACCGG CAGTGCACCA CAAAGCACAA CATCCTGGGG CTCCAATCGA 
GTGGTTACCC TGCCTAGCAT TCAGCAGAGC AACTCACAGC GTGGCCAATC ATCTATGCGA 
TCAAACTCCA GCAGTACTGT CACCATTACT CAAACGAAAA CCAAACAGCA GGAGAGGAAT 
ACAGCGACTC CAGGCGACGG AGAGGAGGTG ATGTGCAACT GTGGGCAGTT GGCTAGCCAG 
CTGACGGTCC GAAAAGACGG ACCAAACCAG GGTAGACCCT TTTATGCCTG TCCCACGCGG 
GAGAAGTCAT GCGGTTTCTT CAAATGGGGA GATGAGGACC AAAACCAAGG TGCTAGCAGC 
ACCTCTTGGG GCTCGGCTAA TCGAAATCCC CCTGGACGCA GTCAGCCCAC AGCTATTACG 
TCCGATGGTC CCAAAACGAG ACGCTGTGGT CTCTGTCGCA AGGAGGGACA CACAAGGAAC 
AAGTGTCCAC GCAAGGATGA GTTTGATATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523602.2
CDS	71..3823
Translation	MKAVLTCFRPVVASHRRYCANPGQAMKYLNVAEKNDAAKTIAGLLSNGAAQRREGYSVYNKVFDFEAPVRGQNAKMVMTSVSGHMMQLAFQVSYKNWRTVDPRSLFDAPVKKGVGSDYEPIKRTLEREVRGCQGLIIWTDCDREGENIGYEIIDVCRAIKPNISVYRATFSEITTVAVRRALQQLGQPDKRQSDAVDVRTELDLRTGAAITRFQTMRLQRLFPEKIADKLISYGSCQIPTLGFVAERYKEIEAFVSEPFWKIKVLHTIDDLTVEFNWARNRLFDKEACENYLLLCLAEPDPRALVESVTVKPKHKWRPTPLDTVEMEKLGSRKLKLSAKETMTIAEKLYTKGFISYPRTETNQFSKEFALAPLVEMQTGHRDWGAFAQRVIEWGPNPRNGNKSDQAHPPIHPTKLAENLQGNEARVYELVVRHFLACVSKDAVGSETLVHIDIAGEKFTANGLVIHERNYLDVYVYDKWSAKQIHHYENGQRFEPTEVSLHEGATTAPPLLTEADLIALMEKHGIGTDATHAEHINTIKERGYIGVLDKGFLVPGVIGMGLYEGYDAMELALAKPQLRAEFELDLKLICQGQKDPKVVLTEQIAKYKQAYQQITDKITAMDAKISARINETPAANSAVQEGADGSAPSHGIIQSIFQCPKCNEAPLALKPKKNQQGWYIGCNNFPDCKNAVWLPTECKDASVLDECCPTCGDGYRMLKFRLSTPYYRGVFGTPSGWYKTCLPCDNLFRTTFNINLDSVKKVGGIVGEVRGGGGGPGPGPGGGGSGRGAGSGGWSSGPGGGGSGGGGGSGGWGSGTGGGGSGGWGSGTGGGGLGGGKGKKPGGESKKSATKKPPNEPKPKKTKEPKAAPNKKTSSKSSGSIRSFFTSAAPTNSASNGLDEFFDSNDGFEDAMLAAAESVESSSQPKTISMVPLDDDIAAAFAADDDAEFEALVNGGTMPTESNGDQQLDKSLSEWIKEQDKADERPMLWGTRERASLGTAAPTPPPKPAAKRPRWDSVERDSTPPSSVPESETVLCTGCQQPARQNTVRKNGPNLGRLYYKCPKPDECNFFQWADEPPSSAKSKNSTGSAPQSTTSWGSNRVVTLPSIQQSNSQRGQSSMRSNSSSTVTITQTKTKQQERNTATPGDGEEVMCNCGQLASQLTVRKDGPNQGRPFYACPTREKSCGFFKWGDEDQNQGASSTSWGSANRNPPGRSQPTAITSDGPKTRRCGLCRKEGHTRNKCPRKDEFDM

Target ORF information:

RefSeq Version	NM_078878.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase 3alpha (Top3alpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078878.3

ORF Insert Sequence:

ATGAAAGCTG TCTTAACATG TTTTCGCCCC GTCGTCGCCT CACACAGAAG ATATTGCGCT 
AATCCCGGCC AGGCAATGAA GTACTTAAAC GTGGCTGAGA AGAACGACGC CGCCAAAACG 
ATTGCCGGCC TGCTGTCAAA TGGCGCAGCA CAAAGGCGCG AAGGCTACTC GGTGTACAAC 
AAGGTCTTCG ATTTCGAGGC CCCTGTGCGC GGTCAGAATG CCAAAATGGT TATGACCTCC 
GTATCCGGCC ACATGATGCA GCTGGCATTC CAGGTGTCTT ACAAAAATTG GAGAACGGTG 
GATCCGCGCT CTTTATTCGA CGCGCCGGTG AAAAAGGGCG TGGGCTCGGA TTATGAGCCT 
ATCAAAAGGA CCTTGGAACG CGAGGTGCGC GGCTGTCAGG GATTGATTAT CTGGACGGAT 
TGCGATCGCG AGGGCGAGAA CATTGGCTAC GAGATTATTG ATGTGTGTCG CGCTATCAAA 
CCGAATATTT CGGTTTATCG TGCCACTTTC TCGGAGATTA CCACGGTGGC GGTGCGTCGG 
GCTCTGCAGC AATTGGGGCA GCCGGACAAA AGGCAGAGCG ATGCAGTGGA TGTGCGCACG 
GAACTGGATT TGCGAACTGG TGCCGCCATC ACCCGGTTTC AGACAATGCG CCTACAACGT 
CTGTTCCCCG AAAAGATAGC TGATAAGCTC ATATCCTATG GAAGCTGCCA AATTCCCACC 
CTGGGATTCG TCGCCGAACG GTACAAGGAG ATTGAGGCCT TCGTATCCGA GCCATTTTGG 
AAGATTAAGG TTCTGCACAC AATTGATGAC TTGACGGTGG AGTTTAATTG GGCCCGAAAC 
CGACTCTTCG ATAAGGAAGC TTGTGAAAAC TATTTGCTCC TCTGCCTGGC CGAACCCGAT 
CCTAGAGCGC TCGTGGAGAG CGTCACCGTT AAGCCCAAAC ACAAATGGCG CCCAACTCCG 
CTGGATACCG TGGAAATGGA GAAGCTTGGC TCGAGGAAAC TCAAGCTATC TGCCAAGGAG 
ACGATGACAA TAGCCGAAAA ATTATATACC AAGGGTTTCA TCAGCTATCC CCGTACGGAA 
ACCAACCAGT TCTCCAAGGA GTTCGCTCTG GCACCACTAG TTGAGATGCA AACGGGTCAC 
CGCGACTGGG GAGCATTTGC CCAGCGGGTG ATTGAGTGGG GACCAAATCC TCGAAACGGT 
AACAAATCGG ATCAGGCCCA TCCTCCAATT CATCCCACCA AGCTGGCAGA AAACCTGCAG 
GGAAACGAGG CGCGAGTGTA CGAACTGGTC GTCCGGCACT TCCTCGCCTG CGTCAGCAAA 
GATGCAGTCG GCTCCGAAAC CCTTGTCCAT ATCGACATTG CCGGTGAGAA GTTCACGGCC 
AATGGTCTGG TTATTCACGA ACGAAACTAC CTAGATGTTT ATGTTTATGA CAAGTGGAGT 
GCCAAGCAGA TCCATCACTA TGAAAACGGG CAGCGTTTTG AGCCCACGGA AGTGTCGCTC 
CACGAAGGTG CCACCACTGC CCCGCCCCTG CTGACCGAAG CGGATTTGAT TGCACTGATG 
GAAAAGCATG GGATTGGTAC AGATGCCACG CACGCTGAGC ACATAAACAC GATCAAGGAA 
CGCGGATACA TCGGAGTGCT GGATAAGGGC TTTTTGGTAC CTGGAGTTAT TGGAATGGGA 
CTGTATGAGG GATACGATGC CATGGAGCTG GCCCTTGCCA AGCCACAACT TCGTGCTGAG 
TTTGAATTGG ATCTGAAACT AATTTGCCAG GGGCAGAAAG ATCCCAAGGT GGTCCTGACC 
GAACAGATAG CCAAGTACAA GCAGGCCTAT CAGCAGATTA CCGATAAGAT TACCGCCATG 
GACGCCAAGA TATCGGCTCG CATTAATGAG ACACCAGCTG CCAATTCTGC GGTGCAGGAA 
GGAGCTGATG GAAGTGCTCC AAGTCACGGC ATCATCCAAT CGATCTTCCA GTGCCCTAAG 
TGCAATGAGG CTCCTCTGGC CCTAAAGCCA AAGAAGAACC AGCAGGGCTG GTACATTGGC 
TGCAATAACT TTCCCGACTG CAAAAACGCA GTTTGGCTTC CAACGGAGTG CAAGGACGCA 
TCTGTGCTGG ACGAGTGCTG TCCCACCTGT GGCGATGGAT ACCGGATGCT CAAGTTCCGC 
CTGAGCACGC CTTACTATCG CGGGGTATTT GGTACTCCCA GCGGTTGGTA CAAAACCTGC 
TTGCCCTGCG ATAATCTCTT TCGCACCACG TTCAACATTA ATTTGGACTC GGTTAAGAAA 
GTGGGAGGCA TTGTGGGTGA AGTGCGTGGT GGAGGAGGGG GTCCAGGCCC AGGACCCGGT 
GGCGGTGGCT CTGGCAGAGG AGCCGGCTCT GGTGGATGGA GCAGCGGACC TGGAGGCGGT 
GGCTCTGGCG GAGGCGGCGG CTCTGGAGGA TGGGGCAGCG GAACTGGAGG CGGTGGCTCT 
GGAGGATGGG GCAGCGGAAC TGGAGGCGGT GGCTTGGGCG GAGGAAAGGG CAAGAAACCT 
GGCGGCGAAT CTAAAAAATC AGCTACAAAA AAACCTCCGA ACGAACCCAA ACCGAAAAAG 
ACGAAAGAAC CGAAAGCGGC ACCCAACAAA AAGACTAGCA GCAAATCGTC TGGCAGCATA 
CGGAGCTTCT TTACAAGTGC GGCGCCTACA AATTCCGCCA GCAATGGACT AGATGAGTTT 
TTCGATAGTA ACGATGGCTT TGAGGACGCC ATGTTGGCTG CTGCCGAATC CGTGGAATCG 
AGTAGCCAAC CCAAAACCAT TAGTATGGTC CCTCTTGATG ACGACATAGC CGCCGCTTTT 
GCAGCGGATG ATGATGCTGA ATTCGAGGCG TTGGTAAACG GCGGAACCAT GCCCACCGAA 
TCCAATGGGG ACCAGCAGCT GGACAAAAGT CTCTCGGAAT GGATAAAGGA GCAGGACAAA 
GCGGACGAGA GGCCCATGCT TTGGGGAACT CGGGAGCGAG CGTCGTTAGG TACTGCTGCT 
CCCACTCCTC CTCCGAAACC GGCAGCTAAA CGACCGAGAT GGGACAGTGT CGAACGGGAT 
TCCACACCAC CCTCTTCGGT TCCGGAATCC GAAACAGTGC TGTGTACCGG ATGTCAGCAG 
CCAGCTCGCC AGAACACTGT CCGTAAGAAT GGACCCAACC TAGGCAGGCT GTACTACAAG 
TGTCCCAAAC CGGATGAGTG CAACTTCTTT CAGTGGGCTG ATGAGCCACC GTCGTCGGCC 
AAAAGCAAGA ATTCCACCGG CAGTGCACCA CAAAGCACAA CATCCTGGGG CTCCAATCGA 
GTGGTTACCC TGCCTAGCAT TCAGCAGAGC AACTCACAGC GTGGCCAATC ATCTATGCGA 
TCAAACTCCA GCAGTACTGT CACCATTACT CAAACGAAAA CCAAACAGCA GGAGAGGAAT 
ACAGCGACTC CAGGCGACGG AGAGGAGGTG ATGTGCAACT GTGGGCAGTT GGCTAGCCAG 
CTGACGGTCC GAAAAGACGG ACCAAACCAG GGTAGACCCT TTTATGCCTG TCCCACGCGG 
GAGAAGTCAT GCGGTTTCTT CAAATGGGGA GATGAGGACC AAAACCAAGG TGCTAGCAGC 
ACCTCTTGGG GCTCGGCTAA TCGAAATCCC CCTGGACGCA GTCAGCCCAC AGCTATTACG 
TCCGATGGTC CCAAAACGAG ACGCTGTGGT CTCTGTCGCA AGGAGGGACA CACAAGGAAC 
AAGTGTCCAC GCAAGGATGA GTTTGATATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Top3alpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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