Gal cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gal
Entrez Gene ID	33839
Full Name	beta galactosidase
Synonyms	CG9092,Dmel\CG9092,beta-GAL,beta-Gal-1,beta-gal,dbeta-Gal,gal,lacZ-1
General protein information	Preferred Names beta galactosidase Names CG9092 gene product from transcript CG9092-RB CG9092-PA CG9092-PB Gal-PA Gal-PB beta-galactosidase-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26B2-26B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273191.1	NP_001260120.1	beta galactosidase, isoform B
NM_135134.3	NP_608978.2	beta galactosidase, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002055	XP_550752.3
Drosophila melanogaster (fruit fly)	Gal	NP_001260120.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gal gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gal cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13855	NM_135134.3 Latest version!	Drosophila melanogaster beta galactosidase (Gal), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa16310	NM_001273191.1 Latest version!	Drosophila melanogaster beta galactosidase (Gal), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gal ( NM_135134.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608978.2 Drosophila melanogaster beta galactosidase (Gal), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13855
Clone ID Related Accession (Same CDS sequence)	NM_135134.3
Accession Version	NM_135134.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	beta galactosidase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135134.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGTT GTCGTCGCAA TCGAAAGCTG ACGATGGCCG TAAGTGGATG TCTGATAATT 
GCTGTGATGG CCCTCACCGT TGGGCTTTGT GTGGGACTGA GCGGTGACAC CGACGCCCCC 
GAGGAACAGC CAAGGTTTAC GATCGACCAT GAGGCCAACA CCTTCATGCT GGATGGCCAG 
CCCTTCCGGT ACGTCTCTGG ATCGTTTCAC TATTTCCGCG CTGTACCCGA GTCCTGGCGA 
AGTCGGTTGC GCACCATGCG AGCATCTGGT CTTAATGCTC TGGATACCTA TGTTGAATGG 
TCGCTCCACA ATCCGCACGA TGGAGAATAC AACTGGGAGG GCATTGCGGA TGTTGTCAAG 
TTTCTGGAGA TTGCTCAGGA GGAAGACTTC TACATCATTC TCCGCCCAGG TCCCTACATT 
TGTGCTGAGC GAGATAATGG TGGTCTTCCT CACTGGTTGT TTACCAAATA CCCGTCCATC 
AAGATGCGGA CCAATGACCC CAACTACATC TCAGAGGTGG GCAAGTGGTA TGCGGAGCTG 
ATGCCACGCC TCCAGCACCT GTTCGTAGGC AACGGCGGCA AGATCATAAT GGTGCAGGTG 
GAGAACGAGT ACGGTGACTA TGCCTGCGAT CACGACTACC TCAACTGGCT GCGAGACGAG 
ACGGAGAAGT ATGTGTCCGG CAAGGCGCTG CTCTTCACGG TGGACATACC CAACGAGAAG 
ATGAGCTGTG GAAAGATCGA GAACGTGTTC GCCACCACTG ACTTCGGCAT CGATCGCATC 
AATGAGATTG ACAAAATCTG GGCAATGCTG CGTGCCCTGC AGCCGACTGG CCCACTGGTC 
AACTCGGAGT TCTATCCCGG CTGGCTAACG CATTGGCAGG AGCAGAACCA AAGGCGTGAT 
GGCCAGGAGG TAGCCAATGC ACTAAGGACA ATTCTGAGCT ACAACGCTAG TGTTAACCTC 
TATATGTTCT TTGGCGGCAC AAACTTTGGG TTCACTGCTG GTGCCAACTA TAATCTGGAC 
GGTGGCATCG GATATGCGGC AGACATAACC AGCTACGACT ACGATGCAGT GATGGACGAG 
GCCGGTGGCG TCACCACTAA GTACAACCTT GTCAAGGCTG TGATTGGAGA ATTCCTTCCG 
CTGCCGGAAA TCACCTTGAA TCCCGCTAAG CGTCTGGCCT ACGGAAGGGT GGAGCTGACA 
CCCAAGCTGA CACTGCTCTC GACGGAAGGA CGTGCTGCGC TCTCCAAGGG TGATCCCGTC 
GAATCGATCA AGCCGAAAAC CTTCGAGGAA CTAGACTTGT ACTCGGGCCT CGTCCTGTAC 
GAGACCGAGC TGCCCAGCAT GGATCTAGAC CCAGCCTTGC TGAAAATAGA TCAAATTAAC 
GATCGAGCAC ACGTATTCGT AGACCAGGAA CTGGTGGGCA CACTCTCCCG CGAGGCACAG 
ATCTACTCGC TGCCGCTTAG CAAGGGTTGG GGCAGCACAC TGCAGCTGCT GGTGGAGAAC 
CAAGGACGCG TCAACTTCTA CATCTCCAAC GACACGAAGG GCATCTTTGG TGAGGTGAGC 
CTGCAGCTGC ACAATGGTGG TTACCTCCCG CTGGAGAACT GGCGGAGCAC CGCTTTCCCG 
CTGGAGCAAT CCGCCGTCGA GCTCTGGCGG CGCGAACACA CTGACGAGAA GGCCTTGGAC 
CCGTTGCTGG CAAGGCAACG CATTCTTCGA AACGGACCAA TACTCTACAC TGGCAGCCTG 
ACAGTGACGG AGGTGGGCGA CACCTATTTG AACATGGCCG GTTGGGGCAA GGGCGTGGCC 
TATGTGAACG GTTTTAATTT GGGACGCTAC TGGCCTGTGG CAGGTCCACA GGTCACCTTA 
TATGTGCCCA ACGAGATACT GAAAGTGGGT GAAAACTCTC TGGTGATCCT TGAGTACCAA 
CGAGCGAATA AGACAGCAAC TGGCGAGGAT CTACCTGCAG TGCAGTTCGA CGCAGTTGCT 
CAGCTGGACG GAGAGTCTGG TGATGTTCCA CTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608978.2
CDS	139..2157
Translation	MSGCRRNRKLTMAVSGCLIIAVMALTVGLCVGLSGDTDAPEEQPRFTIDHEANTFMLDGQPFRYVSGSFHYFRAVPESWRSRLRTMRASGLNALDTYVEWSLHNPHDGEYNWEGIADVVKFLEIAQEEDFYIILRPGPYICAERDNGGLPHWLFTKYPSIKMRTNDPNYISEVGKWYAELMPRLQHLFVGNGGKIIMVQVENEYGDYACDHDYLNWLRDETEKYVSGKALLFTVDIPNEKMSCGKIENVFATTDFGIDRINEIDKIWAMLRALQPTGPLVNSEFYPGWLTHWQEQNQRRDGQEVANALRTILSYNASVNLYMFFGGTNFGFTAGANYNLDGGIGYAADITSYDYDAVMDEAGGVTTKYNLVKAVIGEFLPLPEITLNPAKRLAYGRVELTPKLTLLSTEGRAALSKGDPVESIKPKTFEELDLYSGLVLYETELPSMDLDPALLKIDQINDRAHVFVDQELVGTLSREAQIYSLPLSKGWGSTLQLLVENQGRVNFYISNDTKGIFGEVSLQLHNGGYLPLENWRSTAFPLEQSAVELWRREHTDEKALDPLLARQRILRNGPILYTGSLTVTEVGDTYLNMAGWGKGVAYVNGFNLGRYWPVAGPQVTLYVPNEILKVGENSLVILEYQRANKTATGEDLPAVQFDAVAQLDGESGDVPLK

Target ORF information:

RefSeq Version	NM_135134.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta galactosidase (Gal), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135134.3

ORF Insert Sequence:

ATGAGCGGTT GTCGTCGCAA TCGAAAGCTG ACGATGGCCG TAAGTGGATG TCTGATAATT 
GCTGTGATGG CCCTCACCGT TGGGCTTTGT GTGGGACTGA GCGGTGACAC CGACGCCCCC 
GAGGAACAGC CAAGGTTTAC GATCGACCAT GAGGCCAACA CCTTCATGCT GGATGGCCAG 
CCCTTCCGGT ACGTCTCTGG ATCGTTTCAC TATTTCCGCG CTGTACCCGA GTCCTGGCGA 
AGTCGGTTGC GCACCATGCG AGCATCTGGT CTTAATGCTC TGGATACCTA TGTTGAATGG 
TCGCTCCACA ATCCGCACGA TGGAGAATAC AACTGGGAGG GCATTGCGGA TGTTGTCAAG 
TTTCTGGAGA TTGCTCAGGA GGAAGACTTC TACATCATTC TCCGCCCAGG TCCCTACATT 
TGTGCTGAGC GAGATAATGG TGGTCTTCCT CACTGGTTGT TTACCAAATA CCCGTCCATC 
AAGATGCGGA CCAATGACCC CAACTACATC TCAGAGGTGG GCAAGTGGTA TGCGGAGCTG 
ATGCCACGCC TCCAGCACCT GTTCGTAGGC AACGGCGGCA AGATCATAAT GGTGCAGGTG 
GAGAACGAGT ACGGTGACTA TGCCTGCGAT CACGACTACC TCAACTGGCT GCGAGACGAG 
ACGGAGAAGT ATGTGTCCGG CAAGGCGCTG CTCTTCACGG TGGACATACC CAACGAGAAG 
ATGAGCTGTG GAAAGATCGA GAACGTGTTC GCCACCACTG ACTTCGGCAT CGATCGCATC 
AATGAGATTG ACAAAATCTG GGCAATGCTG CGTGCCCTGC AGCCGACTGG CCCACTGGTC 
AACTCGGAGT TCTATCCCGG CTGGCTAACG CATTGGCAGG AGCAGAACCA AAGGCGTGAT 
GGCCAGGAGG TAGCCAATGC ACTAAGGACA ATTCTGAGCT ACAACGCTAG TGTTAACCTC 
TATATGTTCT TTGGCGGCAC AAACTTTGGG TTCACTGCTG GTGCCAACTA TAATCTGGAC 
GGTGGCATCG GATATGCGGC AGACATAACC AGCTACGACT ACGATGCAGT GATGGACGAG 
GCCGGTGGCG TCACCACTAA GTACAACCTT GTCAAGGCTG TGATTGGAGA ATTCCTTCCG 
CTGCCGGAAA TCACCTTGAA TCCCGCTAAG CGTCTGGCCT ACGGAAGGGT GGAGCTGACA 
CCCAAGCTGA CACTGCTCTC GACGGAAGGA CGTGCTGCGC TCTCCAAGGG TGATCCCGTC 
GAATCGATCA AGCCGAAAAC CTTCGAGGAA CTAGACTTGT ACTCGGGCCT CGTCCTGTAC 
GAGACCGAGC TGCCCAGCAT GGATCTAGAC CCAGCCTTGC TGAAAATAGA TCAAATTAAC 
GATCGAGCAC ACGTATTCGT AGACCAGGAA CTGGTGGGCA CACTCTCCCG CGAGGCACAG 
ATCTACTCGC TGCCGCTTAG CAAGGGTTGG GGCAGCACAC TGCAGCTGCT GGTGGAGAAC 
CAAGGACGCG TCAACTTCTA CATCTCCAAC GACACGAAGG GCATCTTTGG TGAGGTGAGC 
CTGCAGCTGC ACAATGGTGG TTACCTCCCG CTGGAGAACT GGCGGAGCAC CGCTTTCCCG 
CTGGAGCAAT CCGCCGTCGA GCTCTGGCGG CGCGAACACA CTGACGAGAA GGCCTTGGAC 
CCGTTGCTGG CAAGGCAACG CATTCTTCGA AACGGACCAA TACTCTACAC TGGCAGCCTG 
ACAGTGACGG AGGTGGGCGA CACCTATTTG AACATGGCCG GTTGGGGCAA GGGCGTGGCC 
TATGTGAACG GTTTTAATTT GGGACGCTAC TGGCCTGTGG CAGGTCCACA GGTCACCTTA 
TATGTGCCCA ACGAGATACT GAAAGTGGGT GAAAACTCTC TGGTGATCCT TGAGTACCAA 
CGAGCGAATA AGACAGCAAC TGGCGAGGAT CTACCTGCAG TGCAGTTCGA CGCAGTTGCT 
CAGCTGGACG GAGAGTCTGG TGATGTTCCA CTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gal ( NM_001273191.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260120.1 Drosophila melanogaster beta galactosidase (Gal), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16310
Clone ID Related Accession (Same CDS sequence)	NM_001273191.1
Accession Version	NM_001273191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	beta galactosidase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGTT GTCGTCGCAA TCGAAAGCTG ACGATGGCCG TAAGTGGATG TCTGATAATT 
GCTGTGATGG CCCTCACCGT TGGGCTTTGT GTGGGACTGA GCGGTGACAC CGACGCCCCC 
GAGCCAAGGT TTACGATCGA CCATGAGGCC AACACCTTCA TGCTGGATGG CCAGCCCTTC 
CGGTACGTCT CTGGATCGTT TCACTATTTC CGCGCTGTAC CCGAGTCCTG GCGAAGTCGG 
TTGCGCACCA TGCGAGCATC TGGTCTTAAT GCTCTGGATA CCTATGTTGA ATGGTCGCTC 
CACAATCCGC ACGATGGAGA ATACAACTGG GAGGGCATTG CGGATGTTGT CAAGTTTCTG 
GAGATTGCTC AGGAGGAAGA CTTCTACATC ATTCTCCGCC CAGGTCCCTA CATTTGTGCT 
GAGCGAGATA ATGGTGGTCT TCCTCACTGG TTGTTTACCA AATACCCGTC CATCAAGATG 
CGGACCAATG ACCCCAACTA CATCTCAGAG GTGGGCAAGT GGTATGCGGA GCTGATGCCA 
CGCCTCCAGC ACCTGTTCGT AGGCAACGGC GGCAAGATCA TAATGGTGCA GGTGGAGAAC 
GAGTACGGTG ACTATGCCTG CGATCACGAC TACCTCAACT GGCTGCGAGA CGAGACGGAG 
AAGTATGTGT CCGGCAAGGC GCTGCTCTTC ACGGTGGACA TACCCAACGA GAAGATGAGC 
TGTGGAAAGA TCGAGAACGT GTTCGCCACC ACTGACTTCG GCATCGATCG CATCAATGAG 
ATTGACAAAA TCTGGGCAAT GCTGCGTGCC CTGCAGCCGA CTGGCCCACT GGTCAACTCG 
GAGTTCTATC CCGGCTGGCT AACGCATTGG CAGGAGCAGA ACCAAAGGCG TGATGGCCAG 
GAGGTAGCCA ATGCACTAAG GACAATTCTG AGCTACAACG CTAGTGTTAA CCTCTATATG 
TTCTTTGGCG GCACAAACTT TGGGTTCACT GCTGGTGCCA ACTATAATCT GGACGGTGGC 
ATCGGATATG CGGCAGACAT AACCAGCTAC GACTACGATG CAGTGATGGA CGAGGCCGGT 
GGCGTCACCA CTAAGTACAA CCTTGTCAAG GCTGTGATTG GAGAATTCCT TCCGCTGCCG 
GAAATCACCT TGAATCCCGC TAAGCGTCTG GCCTACGGAA GGGTGGAGCT GACACCCAAG 
CTGACACTGC TCTCGACGGA AGGACGTGCT GCGCTCTCCA AGGGTGATCC CGTCGAATCG 
ATCAAGCCGA AAACCTTCGA GGAACTAGAC TTGTACTCGG GCCTCGTCCT GTACGAGACC 
GAGCTGCCCA GCATGGATCT AGACCCAGCC TTGCTGAAAA TAGATCAAAT TAACGATCGA 
GCACACGTAT TCGTAGACCA GGAACTGGTG GGCACACTCT CCCGCGAGGC ACAGATCTAC 
TCGCTGCCGC TTAGCAAGGG TTGGGGCAGC ACACTGCAGC TGCTGGTGGA GAACCAAGGA 
CGCGTCAACT TCTACATCTC CAACGACACG AAGGGCATCT TTGGTGAGGT GAGCCTGCAG 
CTGCACAATG GTGGTTACCT CCCGCTGGAG AACTGGCGGA GCACCGCTTT CCCGCTGGAG 
CAATCCGCCG TCGAGCTCTG GCGGCGCGAA CACACTGACG AGAAGGCCTT GGACCCGTTG 
CTGGCAAGGC AACGCATTCT TCGAAACGGA CCAATACTCT ACACTGGCAG CCTGACAGTG 
ACGGAGGTGG GCGACACCTA TTTGAACATG GCCGGTTGGG GCAAGGGCGT GGCCTATGTG 
AACGGTTTTA ATTTGGGACG CTACTGGCCT GTGGCAGGTC CACAGGTCAC CTTATATGTG 
CCCAACGAGA TACTGAAAGT GGGTGAAAAC TCTCTGGTGA TCCTTGAGTA CCAACGAGCG 
AATAAGACAG CAACTGGCGA GGATCTACCT GCAGTGCAGT TCGACGCAGT TGCTCAGCTG 
GACGGAGAGT CTGGTGATGT TCCACTTAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260120.1
CDS	139..2151
Translation	MSGCRRNRKLTMAVSGCLIIAVMALTVGLCVGLSGDTDAPEPRFTIDHEANTFMLDGQPFRYVSGSFHYFRAVPESWRSRLRTMRASGLNALDTYVEWSLHNPHDGEYNWEGIADVVKFLEIAQEEDFYIILRPGPYICAERDNGGLPHWLFTKYPSIKMRTNDPNYISEVGKWYAELMPRLQHLFVGNGGKIIMVQVENEYGDYACDHDYLNWLRDETEKYVSGKALLFTVDIPNEKMSCGKIENVFATTDFGIDRINEIDKIWAMLRALQPTGPLVNSEFYPGWLTHWQEQNQRRDGQEVANALRTILSYNASVNLYMFFGGTNFGFTAGANYNLDGGIGYAADITSYDYDAVMDEAGGVTTKYNLVKAVIGEFLPLPEITLNPAKRLAYGRVELTPKLTLLSTEGRAALSKGDPVESIKPKTFEELDLYSGLVLYETELPSMDLDPALLKIDQINDRAHVFVDQELVGTLSREAQIYSLPLSKGWGSTLQLLVENQGRVNFYISNDTKGIFGEVSLQLHNGGYLPLENWRSTAFPLEQSAVELWRREHTDEKALDPLLARQRILRNGPILYTGSLTVTEVGDTYLNMAGWGKGVAYVNGFNLGRYWPVAGPQVTLYVPNEILKVGENSLVILEYQRANKTATGEDLPAVQFDAVAQLDGESGDVPLK

Target ORF information:

RefSeq Version	NM_001273191.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta galactosidase (Gal), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273191.1

ORF Insert Sequence:

ATGAGCGGTT GTCGTCGCAA TCGAAAGCTG ACGATGGCCG TAAGTGGATG TCTGATAATT 
GCTGTGATGG CCCTCACCGT TGGGCTTTGT GTGGGACTGA GCGGTGACAC CGACGCCCCC 
GAGCCAAGGT TTACGATCGA CCATGAGGCC AACACCTTCA TGCTGGATGG CCAGCCCTTC 
CGGTACGTCT CTGGATCGTT TCACTATTTC CGCGCTGTAC CCGAGTCCTG GCGAAGTCGG 
TTGCGCACCA TGCGAGCATC TGGTCTTAAT GCTCTGGATA CCTATGTTGA ATGGTCGCTC 
CACAATCCGC ACGATGGAGA ATACAACTGG GAGGGCATTG CGGATGTTGT CAAGTTTCTG 
GAGATTGCTC AGGAGGAAGA CTTCTACATC ATTCTCCGCC CAGGTCCCTA CATTTGTGCT 
GAGCGAGATA ATGGTGGTCT TCCTCACTGG TTGTTTACCA AATACCCGTC CATCAAGATG 
CGGACCAATG ACCCCAACTA CATCTCAGAG GTGGGCAAGT GGTATGCGGA GCTGATGCCA 
CGCCTCCAGC ACCTGTTCGT AGGCAACGGC GGCAAGATCA TAATGGTGCA GGTGGAGAAC 
GAGTACGGTG ACTATGCCTG CGATCACGAC TACCTCAACT GGCTGCGAGA CGAGACGGAG 
AAGTATGTGT CCGGCAAGGC GCTGCTCTTC ACGGTGGACA TACCCAACGA GAAGATGAGC 
TGTGGAAAGA TCGAGAACGT GTTCGCCACC ACTGACTTCG GCATCGATCG CATCAATGAG 
ATTGACAAAA TCTGGGCAAT GCTGCGTGCC CTGCAGCCGA CTGGCCCACT GGTCAACTCG 
GAGTTCTATC CCGGCTGGCT AACGCATTGG CAGGAGCAGA ACCAAAGGCG TGATGGCCAG 
GAGGTAGCCA ATGCACTAAG GACAATTCTG AGCTACAACG CTAGTGTTAA CCTCTATATG 
TTCTTTGGCG GCACAAACTT TGGGTTCACT GCTGGTGCCA ACTATAATCT GGACGGTGGC 
ATCGGATATG CGGCAGACAT AACCAGCTAC GACTACGATG CAGTGATGGA CGAGGCCGGT 
GGCGTCACCA CTAAGTACAA CCTTGTCAAG GCTGTGATTG GAGAATTCCT TCCGCTGCCG 
GAAATCACCT TGAATCCCGC TAAGCGTCTG GCCTACGGAA GGGTGGAGCT GACACCCAAG 
CTGACACTGC TCTCGACGGA AGGACGTGCT GCGCTCTCCA AGGGTGATCC CGTCGAATCG 
ATCAAGCCGA AAACCTTCGA GGAACTAGAC TTGTACTCGG GCCTCGTCCT GTACGAGACC 
GAGCTGCCCA GCATGGATCT AGACCCAGCC TTGCTGAAAA TAGATCAAAT TAACGATCGA 
GCACACGTAT TCGTAGACCA GGAACTGGTG GGCACACTCT CCCGCGAGGC ACAGATCTAC 
TCGCTGCCGC TTAGCAAGGG TTGGGGCAGC ACACTGCAGC TGCTGGTGGA GAACCAAGGA 
CGCGTCAACT TCTACATCTC CAACGACACG AAGGGCATCT TTGGTGAGGT GAGCCTGCAG 
CTGCACAATG GTGGTTACCT CCCGCTGGAG AACTGGCGGA GCACCGCTTT CCCGCTGGAG 
CAATCCGCCG TCGAGCTCTG GCGGCGCGAA CACACTGACG AGAAGGCCTT GGACCCGTTG 
CTGGCAAGGC AACGCATTCT TCGAAACGGA CCAATACTCT ACACTGGCAG CCTGACAGTG 
ACGGAGGTGG GCGACACCTA TTTGAACATG GCCGGTTGGG GCAAGGGCGT GGCCTATGTG 
AACGGTTTTA ATTTGGGACG CTACTGGCCT GTGGCAGGTC CACAGGTCAC CTTATATGTG 
CCCAACGAGA TACTGAAAGT GGGTGAAAAC TCTCTGGTGA TCCTTGAGTA CCAACGAGCG 
AATAAGACAG CAACTGGCGA GGATCTACCT GCAGTGCAGT TCGACGCAGT TGCTCAGCTG 
GACGGAGAGT CTGGTGATGT TCCACTTAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gal cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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