colt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	colt
Entrez Gene ID	33470
Full Name	congested-like trachea
Synonyms	CG3057,Dmel\CG3057
General protein information	Preferred Names congested-like trachea Names CG3057 gene product from transcript CG3057-RA CG3057-PA CG3057-PD collapsed trachea colt-PA colt-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23A5-23A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_205894.1	NP_995616.1	congested-like trachea, isoform D
NM_057873.5	NP_477221.1	congested-like trachea, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SLC25A20	XP_863142.1
Gallus gallus (chicken)	SLC25A20	XP_414400.1
Xenopus tropicalis (tropical clawed frog)	slc25a20.2	NP_989099.1
Neurospora crassa	NCU07263	XP_962117.1
Rattus norvegicus (Norway rat)	Slc25a20	NP_446417.2
Caenorhabditis elegans (roundworm)	dif-1	NP_501223.1
Homo sapiens (human)	SLC25A20	NP_000378.1
Macaca mulatta (Rhesus monkey)	SLC25A20	XP_001111751.1
Bos taurus (cattle)	SLC25A20	NP_001071404.1
Mus musculus (house mouse)	Slc25a20	NP_065266.1
Danio rerio (zebrafish)	slc25a20	NP_957153.1
Pan troglodytes (chimpanzee)	SLC25A20	XP_516446.3
Saccharomyces cerevisiae (baker's yeast)	CRC1	NP_014743.1
Arabidopsis thaliana (thale cress)	BOU	NP_568670.1
Kluyveromyces lactis	KLLA0C13431g	XP_452799.1
Eremothecium gossypii	AGOS_ADL264C	NP_983832.1
Oryza sativa (rice)	Os10g0573700	NP_001065471.1
Drosophila melanogaster (fruit fly)	colt	NP_477221.1
Magnaporthe oryzae (rice blast fungus)	MGG_10492	XP_003713396.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006104	XP_316164.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following colt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the colt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13540	NM_205894.1 Latest version!	Drosophila melanogaster congested-like trachea (colt), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa13540	NM_057873.5 Latest version!	Drosophila melanogaster congested-like trachea (colt), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

colt ( NM_205894.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995616.1 Drosophila melanogaster congested-like trachea (colt), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa13540
Clone ID Related Accession (Same CDS sequence)	NM_205894.1 , NM_057873.5
Accession Version	NM_205894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	congested-like trachea, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCACCA CGGAGAACGT GTCCACGGAG CGCAAAGCGA ATCCAGTGAA GTCCTTCCTG 
ACCGGCGGAT TCGGTGGGAT CTGCAATGTG CTCTCTGGCC ATCCGCTGGA CACCATCAAG 
GTGCGCCTGC AGACCATGCC GCGTCCGGCG CCGGGTGAGC AGCCCCTGTA CCGCGGCACC 
TTCGACTGTG CGGCCAAGAC GATCAAAAAT GAGGGCGTCC GCGGTCTGTA CAAGGGCATG 
TCCGCACCGT TGACGGGCGT TGCTCCGATC TTTGCGATGT GCTTCGCCGG CTATGCGCTG 
GGCAAGCGGC TGCAGCAGCG CGGCGAGGAC GCCAAGCTGA CCTACCCGCA AATCTTCGTG 
GCCGGCTCCT TCTCCGGCCT GTTCTCCACC CTGATAATGG CACCCGGCGA GCGCATCAAG 
GTGCTCCTGC AGACGCAGCA GGGTCAGGGT GGCGAGCGCA AGTACAACGG CATGATCGAC 
TGCGCCGGCA AGCTATACAA GGAGGGTGGA CTTCGCAGCG TTTTCAAGGG CAGCTGCGCC 
ACAATGCTCA GGGATTTGCC CGCCAACGGG CTGTACTTCC TGGTCTACGA GGCGCTGCAG 
GATGTGGCCA AGTCGAAGTC AGAGACGGGA CAAATCAGTA CTGCCTCAAC GATTTTCGCA 
GGTGGAGTGG CGGGCATGGC CTACTGGATT CTGGGCATGC CAGCCGACGT GTTAAAGAGC 
CGCCTACAAT CGGCTCCCGA AGGCACCTAC AAGCACGGCA TACGCAGTGT CTTCAAGGAT 
CTGATTGTCA AGGATGGACC TCTGGCCTTG TACCGCGGCG TGACGCCCAT CATGCTCCGA 
GCCTTCCCAG CCAACGCTGC CTGCTTCTTC GGCATTGAGT TGGCTAACAA ATTCTTTAAC 
ATCGTAGCTC CAAATTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995616.1
CDS	502..1422
Translation	MTTTENVSTERKANPVKSFLTGGFGGICNVLSGHPLDTIKVRLQTMPRPAPGEQPLYRGTFDCAAKTIKNEGVRGLYKGMSAPLTGVAPIFAMCFAGYALGKRLQQRGEDAKLTYPQIFVAGSFSGLFSTLIMAPGERIKVLLQTQQGQGGERKYNGMIDCAGKLYKEGGLRSVFKGSCATMLRDLPANGLYFLVYEALQDVAKSKSETGQISTASTIFAGGVAGMAYWILGMPADVLKSRLQSAPEGTYKHGIRSVFKDLIVKDGPLALYRGVTPIMLRAFPANAACFFGIELANKFFNIVAPNF

Target ORF information:

RefSeq Version	NM_205894.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster congested-like trachea (colt), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205894.1

ORF Insert Sequence:

ATGACCACCA CGGAGAACGT GTCCACGGAG CGCAAAGCGA ATCCAGTGAA GTCCTTCCTG 
ACCGGCGGAT TCGGTGGGAT CTGCAATGTG CTCTCTGGCC ATCCGCTGGA CACCATCAAG 
GTGCGCCTGC AGACCATGCC GCGTCCGGCG CCGGGTGAGC AGCCCCTGTA CCGCGGCACC 
TTCGACTGTG CGGCCAAGAC GATCAAAAAT GAGGGCGTCC GCGGTCTGTA CAAGGGCATG 
TCCGCACCGT TGACGGGCGT TGCTCCGATC TTTGCGATGT GCTTCGCCGG CTATGCGCTG 
GGCAAGCGGC TGCAGCAGCG CGGCGAGGAC GCCAAGCTGA CCTACCCGCA AATCTTCGTG 
GCCGGCTCCT TCTCCGGCCT GTTCTCCACC CTGATAATGG CACCCGGCGA GCGCATCAAG 
GTGCTCCTGC AGACGCAGCA GGGTCAGGGT GGCGAGCGCA AGTACAACGG CATGATCGAC 
TGCGCCGGCA AGCTATACAA GGAGGGTGGA CTTCGCAGCG TTTTCAAGGG CAGCTGCGCC 
ACAATGCTCA GGGATTTGCC CGCCAACGGG CTGTACTTCC TGGTCTACGA GGCGCTGCAG 
GATGTGGCCA AGTCGAAGTC AGAGACGGGA CAAATCAGTA CTGCCTCAAC GATTTTCGCA 
GGTGGAGTGG CGGGCATGGC CTACTGGATT CTGGGCATGC CAGCCGACGT GTTAAAGAGC 
CGCCTACAAT CGGCTCCCGA AGGCACCTAC AAGCACGGCA TACGCAGTGT CTTCAAGGAT 
CTGATTGTCA AGGATGGACC TCTGGCCTTG TACCGCGGCG TGACGCCCAT CATGCTCCGA 
GCCTTCCCAG CCAACGCTGC CTGCTTCTTC GGCATTGAGT TGGCTAACAA ATTCTTTAAC 
ATCGTAGCTC CAAATTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

colt ( NM_057873.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477221.1 Drosophila melanogaster congested-like trachea (colt), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13540
Clone ID Related Accession (Same CDS sequence)	NM_205894.1 , NM_057873.5
Accession Version	NM_057873.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	congested-like trachea, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057873.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCACCA CGGAGAACGT GTCCACGGAG CGCAAAGCGA ATCCAGTGAA GTCCTTCCTG 
ACCGGCGGAT TCGGTGGGAT CTGCAATGTG CTCTCTGGCC ATCCGCTGGA CACCATCAAG 
GTGCGCCTGC AGACCATGCC GCGTCCGGCG CCGGGTGAGC AGCCCCTGTA CCGCGGCACC 
TTCGACTGTG CGGCCAAGAC GATCAAAAAT GAGGGCGTCC GCGGTCTGTA CAAGGGCATG 
TCCGCACCGT TGACGGGCGT TGCTCCGATC TTTGCGATGT GCTTCGCCGG CTATGCGCTG 
GGCAAGCGGC TGCAGCAGCG CGGCGAGGAC GCCAAGCTGA CCTACCCGCA AATCTTCGTG 
GCCGGCTCCT TCTCCGGCCT GTTCTCCACC CTGATAATGG CACCCGGCGA GCGCATCAAG 
GTGCTCCTGC AGACGCAGCA GGGTCAGGGT GGCGAGCGCA AGTACAACGG CATGATCGAC 
TGCGCCGGCA AGCTATACAA GGAGGGTGGA CTTCGCAGCG TTTTCAAGGG CAGCTGCGCC 
ACAATGCTCA GGGATTTGCC CGCCAACGGG CTGTACTTCC TGGTCTACGA GGCGCTGCAG 
GATGTGGCCA AGTCGAAGTC AGAGACGGGA CAAATCAGTA CTGCCTCAAC GATTTTCGCA 
GGTGGAGTGG CGGGCATGGC CTACTGGATT CTGGGCATGC CAGCCGACGT GTTAAAGAGC 
CGCCTACAAT CGGCTCCCGA AGGCACCTAC AAGCACGGCA TACGCAGTGT CTTCAAGGAT 
CTGATTGTCA AGGATGGACC TCTGGCCTTG TACCGCGGCG TGACGCCCAT CATGCTCCGA 
GCCTTCCCAG CCAACGCTGC CTGCTTCTTC GGCATTGAGT TGGCTAACAA ATTCTTTAAC 
ATCGTAGCTC CAAATTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477221.1
CDS	505..1425
Translation	MTTTENVSTERKANPVKSFLTGGFGGICNVLSGHPLDTIKVRLQTMPRPAPGEQPLYRGTFDCAAKTIKNEGVRGLYKGMSAPLTGVAPIFAMCFAGYALGKRLQQRGEDAKLTYPQIFVAGSFSGLFSTLIMAPGERIKVLLQTQQGQGGERKYNGMIDCAGKLYKEGGLRSVFKGSCATMLRDLPANGLYFLVYEALQDVAKSKSETGQISTASTIFAGGVAGMAYWILGMPADVLKSRLQSAPEGTYKHGIRSVFKDLIVKDGPLALYRGVTPIMLRAFPANAACFFGIELANKFFNIVAPNF

Target ORF information:

RefSeq Version	NM_057873.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster congested-like trachea (colt), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057873.5

ORF Insert Sequence:

ATGACCACCA CGGAGAACGT GTCCACGGAG CGCAAAGCGA ATCCAGTGAA GTCCTTCCTG 
ACCGGCGGAT TCGGTGGGAT CTGCAATGTG CTCTCTGGCC ATCCGCTGGA CACCATCAAG 
GTGCGCCTGC AGACCATGCC GCGTCCGGCG CCGGGTGAGC AGCCCCTGTA CCGCGGCACC 
TTCGACTGTG CGGCCAAGAC GATCAAAAAT GAGGGCGTCC GCGGTCTGTA CAAGGGCATG 
TCCGCACCGT TGACGGGCGT TGCTCCGATC TTTGCGATGT GCTTCGCCGG CTATGCGCTG 
GGCAAGCGGC TGCAGCAGCG CGGCGAGGAC GCCAAGCTGA CCTACCCGCA AATCTTCGTG 
GCCGGCTCCT TCTCCGGCCT GTTCTCCACC CTGATAATGG CACCCGGCGA GCGCATCAAG 
GTGCTCCTGC AGACGCAGCA GGGTCAGGGT GGCGAGCGCA AGTACAACGG CATGATCGAC 
TGCGCCGGCA AGCTATACAA GGAGGGTGGA CTTCGCAGCG TTTTCAAGGG CAGCTGCGCC 
ACAATGCTCA GGGATTTGCC CGCCAACGGG CTGTACTTCC TGGTCTACGA GGCGCTGCAG 
GATGTGGCCA AGTCGAAGTC AGAGACGGGA CAAATCAGTA CTGCCTCAAC GATTTTCGCA 
GGTGGAGTGG CGGGCATGGC CTACTGGATT CTGGGCATGC CAGCCGACGT GTTAAAGAGC 
CGCCTACAAT CGGCTCCCGA AGGCACCTAC AAGCACGGCA TACGCAGTGT CTTCAAGGAT 
CTGATTGTCA AGGATGGACC TCTGGCCTTG TACCGCGGCG TGACGCCCAT CATGCTCCGA 
GCCTTCCCAG CCAACGCTGC CTGCTTCTTC GGCATTGAGT TGGCTAACAA ATTCTTTAAC 
ATCGTAGCTC CAAATTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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colt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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