Hrs cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hrs
Entrez Gene ID	33458
Full Name	Hepatocyte growth factor regulated tyrosine kinase substrate
Synonyms	CG2903,DmHRS,Dmel\CG2903,HRS,HRS-2,Vps27,dHrs,hrs,l(2)23AB5,l(2)23Ad,l(2)23Ad(Hrs),vps27
General protein information	Preferred Names Hepatocyte growth factor regulated tyrosine kinase substrate Names CG2903 gene product from transcript CG2903-RC CG2903-PA CG2903-PB CG2903-PC F[[1]]F[[0]] ATPase HGF-regulated tyrosine kinase substrate Hrs-PA Hrs-PB Hrs-PC hepatocyte growth factor regulated tyrosine kinase hepatocyte growth factor regulated tyrosine kinase substrate hepatocyte growth factor-regulated tyrosine kinase substrate hepatocyte growth factor-regulated tyrosine substrate hepatocyte growth-factor-regulated substrate
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23A3-23A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_080360.5	NP_525099.3	hepatocyte growth factor regulated tyrosine kinase substrate, isoform B
NM_164497.1	NP_722831.1	hepatocyte growth factor regulated tyrosine kinase substrate, isoform A
NM_164496.1	NP_722830.2	hepatocyte growth factor regulated tyrosine kinase substrate, isoform C

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	HGS	XP_001111673.1
Bos taurus (cattle)	HGS	NP_001039554.1
Danio rerio (zebrafish)	hgs	NP_956162.1
Caenorhabditis elegans (roundworm)	hgrs-1	NP_501375.2
Canis lupus familiaris (dog)	HGS	XP_005624060.1
Mus musculus (house mouse)	Hgs	NP_001152800.1
Gallus gallus (chicken)	HGS	XP_426233.3
Drosophila melanogaster (fruit fly)	Hrs	NP_525099.3
Pan troglodytes (chimpanzee)	HGS	XP_511742.4
Rattus norvegicus (Norway rat)	Hgs	NP_062260.2
Xenopus tropicalis (tropical clawed frog)	hgs	NP_001016513.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008887	XP_319634.4
Homo sapiens (human)	HGS	NP_004703.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hrs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hrs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13529	NM_080360.5 Latest version!	Drosophila melanogaster hepatocyte growth factor regulated tyrosine kinase substrate, transcript variant B (Hrs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa13529	NM_164496.1 Latest version!	Drosophila melanogaster hepatocyte growth factor regulated tyrosine kinase substrate, transcript variant C (Hrs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa13530	NM_164497.1 Latest version!	Drosophila melanogaster hepatocyte growth factor regulated tyrosine kinase substrate, transcript variant A (Hrs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hrs ( NM_080360.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525099.3 Drosophila melanogaster hepatocyte growth factor regulated tyrosine kinase substrate, transcript variant B (Hrs), mRNA.

Price & Availability↑

CloneID	OFa13529
Clone ID Related Accession (Same CDS sequence)	NM_080360.5 , NM_164496.1
Accession Version	NM_080360.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	hepatocyte growth factor regulated tyrosine kinase substrate, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_080360.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCGAT CAAGTTTCGA CAAAAACTTA GAAAATGCCA CCAGTCACTT GCGTCTGGAG 
CCCGACTGGC CATCAATTCT TCTGATTTGC GATGAAATCA ACCAGAAAGA TGTCACTCCC 
AAGAATGCCT TTGCCGCCAT CAAGAAGAAG ATGAACTCGC CCAACCCGCA CTCCTCCTGC 
TATTCCCTCC TGGTCCTCGA GAGCATCGTC AAGAACTGCG GCGCACCCGT TCACGAGGAG 
GTGTTCACCA AGGAGAACTG CGAGATGTTC AGCAGTTTCC TCGAGTCGAC GCCCCACGAG 
AACGTGCGCC AGAAGATGCT GGAGCTGGTG CAGACCTGGG CGTACGCCTT CCGCTCCTCG 
GACAAGTACC AGGCCATCAA GGACACCATG ACCATCCTGA AGGCGAAGGG GCACACTTTC 
CCCGAACTTC GGGAGGCAGA TGCCATGTTC ACGGCGGACA CGGCGCCCAA CTGGGCAGAT 
GGCAGGGTAT GCCATCGCTG TCGCGTGGAG TTCACCTTCA CCAACCGGAA GCACCACTGC 
CGGAACTGCG GCCAGGTCTT CTGTGGCCAA TGCACGGCCA AGCAGTGTCC GCTGCCCAAG 
TACGGCATCG AAAAGGAGGT GCGTGTGTGC GACGGCTGCT TCGCTGCCCT CCAGCGTCCG 
ACGAGCGGCA GTGGAGGCGC TAAGTCCGGA CCACGTCCGG CGGACAGCGA GCTACCGGCG 
GAGTATCTGA ACAGCACTTT GGCGCAGCAG GTGCAGACAC CAGCTCGCAA GACCGAGCAG 
GAACTCAAGG AGGAGGAGGA ACTGCAGCTG GCGCTGGCCC TCAGTCAATC GGAAGCGGAG 
CAGCAGAAGC CCAAGCTGCA ATCCCTGCCA CCCGCTGCCT ATCGCATGCA GCAAAGGTCT 
CCCAGTCCGG AGGCACCGCC AGAACCCAAG GAGTATCACC AGCAGCCGGA GGAGGCTACC 
AATCCGGAGC TGGCCAAGTA CTTGAACCGC AGCTACTGGG AGCAGCGCAA GATCAGCGAA 
TCGTCATCCA TGGCAAGTCC CTCGGCACCG AGCCCCATGC CACCAACGCC GCAGCCCCAG 
CAGATCATGC CCCTGCAGGT CAAGAGTGCG GATGAAGTGC AGATCGATGA GTTTGCCGCC 
AATATGCGCA CGCAGGTGGA GATCTTTGTT AACCGCATGA AGTCGAACTC GAGCCGCGGT 
CGCAGCATCT CCAACGACTC CTCTGTGCAG ACCCTCTTCA TGACCCTGAC CTCGTTGCAC 
TCGCAGCAGT TGTCGTACAT CAAGGAGATG GACGACAAGC GCATGTGGTA CGAGCAGCTG 
CAGGACAAGC TAACCCAGAT CAAGGACTCG CGTGCTGCGC TGGATCAGCT GCGTCAGGAG 
CATGTGGAGA AGCTGCGCAG GATCGCCGAG GAGCAGGAAC GTCAGCGACA GATGCAGATG 
GCCCAGAAAC TGGACATAAT GCGCAAGAAG AAGCAGGAGT ATCTGCAGTA TCAACGACAG 
CTAGCATTGC AGCGCATCCA GGAGCAGGAG CGCGAGATGC AGCTGCGCCA GGAGCAGCAG 
AAGGCGCAGT ATTTGATGGG CCAGTCGGCT CCACCTTTCC CCTACATGCC ACCATCGGCG 
GTGCCACAAC ATGGTTCGCC CTCGCATCAG CTCAACAACG TTTATAATCC CTATGCTGCC 
GGTGTGCCCG GCTATTTGCC TCAGGGTCCT GCACCGGCTC CCAACGGACA TGGCCAGTTT 
CAGGCCATTC CGCCGGGCAT GTACAATCCG GCAATACAGC AGCCGATGCC GCCGAATCTC 
CAGCCCGGCG GATTAATGCA ACAGCCCGCG CCACCTGGAA ATCCCCAGAT GATGCCGCCC 
ATGCCCGAAA ATCAGTTTGC CAACAATCCT GCTGCTATCC TCCAGCTGCC GCAGCAGCAT 
TCGATCGCCC AGCCTCCTCA AATACCATTC CAACCGCAGC CTCAACAGAT TCCTGGTCAG 
CAGCCTCAAC AGATTCCTGG TCAGCAGCCT CAACAGATTC CTGGTCAGCA GCCTCAACAG 
ATTCCTGGTC AGCAGCCACA GCAGATTCCT GTCCAGCAAC CTCAGCCACA ACCCCAAATG 
GGTCACGTAA TGCTGCAGCA GCACCAGGCT CCTCCAGCAG CACAGGCACC TCCAGTTACC 
GAAATCGCCA ACAATCAAGT CCAAGCGGTG GCTGCTGCAC CTGCTCCGCC GCAAAACGAA 
CCGGGACCCG CGCCCGTTAA AGCTGAGGAG CCGGCTACGG CGGAACTGAT TAGCTTTGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525099.3
CDS	140..2422
Translation	MFRSSFDKNLENATSHLRLEPDWPSILLICDEINQKDVTPKNAFAAIKKKMNSPNPHSSCYSLLVLESIVKNCGAPVHEEVFTKENCEMFSSFLESTPHENVRQKMLELVQTWAYAFRSSDKYQAIKDTMTILKAKGHTFPELREADAMFTADTAPNWADGRVCHRCRVEFTFTNRKHHCRNCGQVFCGQCTAKQCPLPKYGIEKEVRVCDGCFAALQRPTSGSGGAKSGPRPADSELPAEYLNSTLAQQVQTPARKTEQELKEEEELQLALALSQSEAEQQKPKLQSLPPAAYRMQQRSPSPEAPPEPKEYHQQPEEATNPELAKYLNRSYWEQRKISESSSMASPSAPSPMPPTPQPQQIMPLQVKSADEVQIDEFAANMRTQVEIFVNRMKSNSSRGRSISNDSSVQTLFMTLTSLHSQQLSYIKEMDDKRMWYEQLQDKLTQIKDSRAALDQLRQEHVEKLRRIAEEQERQRQMQMAQKLDIMRKKKQEYLQYQRQLALQRIQEQEREMQLRQEQQKAQYLMGQSAPPFPYMPPSAVPQHGSPSHQLNNVYNPYAAGVPGYLPQGPAPAPNGHGQFQAIPPGMYNPAIQQPMPPNLQPGGLMQQPAPPGNPQMMPPMPENQFANNPAAILQLPQQHSIAQPPQIPFQPQPQQIPGQQPQQIPGQQPQQIPGQQPQQIPGQQPQQIPVQQPQPQPQMGHVMLQQHQAPPAAQAPPVTEIANNQVQAVAAAPAPPQNEPGPAPVKAEEPATAELISFD

Target ORF information:

RefSeq Version	NM_080360.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hepatocyte growth factor regulated tyrosine kinase substrate, transcript variant B (Hrs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080360.5

ORF Insert Sequence:

ATGTTTCGAT CAAGTTTCGA CAAAAACTTA GAAAATGCCA CCAGTCACTT GCGTCTGGAG 
CCCGACTGGC CATCAATTCT TCTGATTTGC GATGAAATCA ACCAGAAAGA TGTCACTCCC 
AAGAATGCCT TTGCCGCCAT CAAGAAGAAG ATGAACTCGC CCAACCCGCA CTCCTCCTGC 
TATTCCCTCC TGGTCCTCGA GAGCATCGTC AAGAACTGCG GCGCACCCGT TCACGAGGAG 
GTGTTCACCA AGGAGAACTG CGAGATGTTC AGCAGTTTCC TCGAGTCGAC GCCCCACGAG 
AACGTGCGCC AGAAGATGCT GGAGCTGGTG CAGACCTGGG CGTACGCCTT CCGCTCCTCG 
GACAAGTACC AGGCCATCAA GGACACCATG ACCATCCTGA AGGCGAAGGG GCACACTTTC 
CCCGAACTTC GGGAGGCAGA TGCCATGTTC ACGGCGGACA CGGCGCCCAA CTGGGCAGAT 
GGCAGGGTAT GCCATCGCTG TCGCGTGGAG TTCACCTTCA CCAACCGGAA GCACCACTGC 
CGGAACTGCG GCCAGGTCTT CTGTGGCCAA TGCACGGCCA AGCAGTGTCC GCTGCCCAAG 
TACGGCATCG AAAAGGAGGT GCGTGTGTGC GACGGCTGCT TCGCTGCCCT CCAGCGTCCG 
ACGAGCGGCA GTGGAGGCGC TAAGTCCGGA CCACGTCCGG CGGACAGCGA GCTACCGGCG 
GAGTATCTGA ACAGCACTTT GGCGCAGCAG GTGCAGACAC CAGCTCGCAA GACCGAGCAG 
GAACTCAAGG AGGAGGAGGA ACTGCAGCTG GCGCTGGCCC TCAGTCAATC GGAAGCGGAG 
CAGCAGAAGC CCAAGCTGCA ATCCCTGCCA CCCGCTGCCT ATCGCATGCA GCAAAGGTCT 
CCCAGTCCGG AGGCACCGCC AGAACCCAAG GAGTATCACC AGCAGCCGGA GGAGGCTACC 
AATCCGGAGC TGGCCAAGTA CTTGAACCGC AGCTACTGGG AGCAGCGCAA GATCAGCGAA 
TCGTCATCCA TGGCAAGTCC CTCGGCACCG AGCCCCATGC CACCAACGCC GCAGCCCCAG 
CAGATCATGC CCCTGCAGGT CAAGAGTGCG GATGAAGTGC AGATCGATGA GTTTGCCGCC 
AATATGCGCA CGCAGGTGGA GATCTTTGTT AACCGCATGA AGTCGAACTC GAGCCGCGGT 
CGCAGCATCT CCAACGACTC CTCTGTGCAG ACCCTCTTCA TGACCCTGAC CTCGTTGCAC 
TCGCAGCAGT TGTCGTACAT CAAGGAGATG GACGACAAGC GCATGTGGTA CGAGCAGCTG 
CAGGACAAGC TAACCCAGAT CAAGGACTCG CGTGCTGCGC TGGATCAGCT GCGTCAGGAG 
CATGTGGAGA AGCTGCGCAG GATCGCCGAG GAGCAGGAAC GTCAGCGACA GATGCAGATG 
GCCCAGAAAC TGGACATAAT GCGCAAGAAG AAGCAGGAGT ATCTGCAGTA TCAACGACAG 
CTAGCATTGC AGCGCATCCA GGAGCAGGAG CGCGAGATGC AGCTGCGCCA GGAGCAGCAG 
AAGGCGCAGT ATTTGATGGG CCAGTCGGCT CCACCTTTCC CCTACATGCC ACCATCGGCG 
GTGCCACAAC ATGGTTCGCC CTCGCATCAG CTCAACAACG TTTATAATCC CTATGCTGCC 
GGTGTGCCCG GCTATTTGCC TCAGGGTCCT GCACCGGCTC CCAACGGACA TGGCCAGTTT 
CAGGCCATTC CGCCGGGCAT GTACAATCCG GCAATACAGC AGCCGATGCC GCCGAATCTC 
CAGCCCGGCG GATTAATGCA ACAGCCCGCG CCACCTGGAA ATCCCCAGAT GATGCCGCCC 
ATGCCCGAAA ATCAGTTTGC CAACAATCCT GCTGCTATCC TCCAGCTGCC GCAGCAGCAT 
TCGATCGCCC AGCCTCCTCA AATACCATTC CAACCGCAGC CTCAACAGAT TCCTGGTCAG 
CAGCCTCAAC AGATTCCTGG TCAGCAGCCT CAACAGATTC CTGGTCAGCA GCCTCAACAG 
ATTCCTGGTC AGCAGCCACA GCAGATTCCT GTCCAGCAAC CTCAGCCACA ACCCCAAATG 
GGTCACGTAA TGCTGCAGCA GCACCAGGCT CCTCCAGCAG CACAGGCACC TCCAGTTACC 
GAAATCGCCA ACAATCAAGT CCAAGCGGTG GCTGCTGCAC CTGCTCCGCC GCAAAACGAA 
CCGGGACCCG CGCCCGTTAA AGCTGAGGAG CCGGCTACGG CGGAACTGAT TAGCTTTGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hrs ( NM_164496.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722830.2 Drosophila melanogaster hepatocyte growth factor regulated tyrosine kinase substrate, transcript variant C (Hrs), mRNA.

Price & Availability↑

CloneID	OFa13529
Clone ID Related Accession (Same CDS sequence)	NM_080360.5 , NM_164496.1
Accession Version	NM_164496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	hepatocyte growth factor regulated tyrosine kinase substrate, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCGAT CAAGTTTCGA CAAAAACTTA GAAAATGCCA CCAGTCACTT GCGTCTGGAG 
CCCGACTGGC CATCAATTCT TCTGATTTGC GATGAAATCA ACCAGAAAGA TGTCACTCCC 
AAGAATGCCT TTGCCGCCAT CAAGAAGAAG ATGAACTCGC CCAACCCGCA CTCCTCCTGC 
TATTCCCTCC TGGTCCTCGA GAGCATCGTC AAGAACTGCG GCGCACCCGT TCACGAGGAG 
GTGTTCACCA AGGAGAACTG CGAGATGTTC AGCAGTTTCC TCGAGTCGAC GCCCCACGAG 
AACGTGCGCC AGAAGATGCT GGAGCTGGTG CAGACCTGGG CGTACGCCTT CCGCTCCTCG 
GACAAGTACC AGGCCATCAA GGACACCATG ACCATCCTGA AGGCGAAGGG GCACACTTTC 
CCCGAACTTC GGGAGGCAGA TGCCATGTTC ACGGCGGACA CGGCGCCCAA CTGGGCAGAT 
GGCAGGGTAT GCCATCGCTG TCGCGTGGAG TTCACCTTCA CCAACCGGAA GCACCACTGC 
CGGAACTGCG GCCAGGTCTT CTGTGGCCAA TGCACGGCCA AGCAGTGTCC GCTGCCCAAG 
TACGGCATCG AAAAGGAGGT GCGTGTGTGC GACGGCTGCT TCGCTGCCCT CCAGCGTCCG 
ACGAGCGGCA GTGGAGGCGC TAAGTCCGGA CCACGTCCGG CGGACAGCGA GCTACCGGCG 
GAGTATCTGA ACAGCACTTT GGCGCAGCAG GTGCAGACAC CAGCTCGCAA GACCGAGCAG 
GAACTCAAGG AGGAGGAGGA ACTGCAGCTG GCGCTGGCCC TCAGTCAATC GGAAGCGGAG 
CAGCAGAAGC CCAAGCTGCA ATCCCTGCCA CCCGCTGCCT ATCGCATGCA GCAAAGGTCT 
CCCAGTCCGG AGGCACCGCC AGAACCCAAG GAGTATCACC AGCAGCCGGA GGAGGCTACC 
AATCCGGAGC TGGCCAAGTA CTTGAACCGC AGCTACTGGG AGCAGCGCAA GATCAGCGAA 
TCGTCATCCA TGGCAAGTCC CTCGGCACCG AGCCCCATGC CACCAACGCC GCAGCCCCAG 
CAGATCATGC CCCTGCAGGT CAAGAGTGCG GATGAAGTGC AGATCGATGA GTTTGCCGCC 
AATATGCGCA CGCAGGTGGA GATCTTTGTT AACCGCATGA AGTCGAACTC GAGCCGCGGT 
CGCAGCATCT CCAACGACTC CTCTGTGCAG ACCCTCTTCA TGACCCTGAC CTCGTTGCAC 
TCGCAGCAGT TGTCGTACAT CAAGGAGATG GACGACAAGC GCATGTGGTA CGAGCAGCTG 
CAGGACAAGC TAACCCAGAT CAAGGACTCG CGTGCTGCGC TGGATCAGCT GCGTCAGGAG 
CATGTGGAGA AGCTGCGCAG GATCGCCGAG GAGCAGGAAC GTCAGCGACA GATGCAGATG 
GCCCAGAAAC TGGACATAAT GCGCAAGAAG AAGCAGGAGT ATCTGCAGTA TCAACGACAG 
CTAGCATTGC AGCGCATCCA GGAGCAGGAG CGCGAGATGC AGCTGCGCCA GGAGCAGCAG 
AAGGCGCAGT ATTTGATGGG CCAGTCGGCT CCACCTTTCC CCTACATGCC ACCATCGGCG 
GTGCCACAAC ATGGTTCGCC CTCGCATCAG CTCAACAACG TTTATAATCC CTATGCTGCC 
GGTGTGCCCG GCTATTTGCC TCAGGGTCCT GCACCGGCTC CCAACGGACA TGGCCAGTTT 
CAGGCCATTC CGCCGGGCAT GTACAATCCG GCAATACAGC AGCCGATGCC GCCGAATCTC 
CAGCCCGGCG GATTAATGCA ACAGCCCGCG CCACCTGGAA ATCCCCAGAT GATGCCGCCC 
ATGCCCGAAA ATCAGTTTGC CAACAATCCT GCTGCTATCC TCCAGCTGCC GCAGCAGCAT 
TCGATCGCCC AGCCTCCTCA AATACCATTC CAACCGCAGC CTCAACAGAT TCCTGGTCAG 
CAGCCTCAAC AGATTCCTGG TCAGCAGCCT CAACAGATTC CTGGTCAGCA GCCTCAACAG 
ATTCCTGGTC AGCAGCCACA GCAGATTCCT GTCCAGCAAC CTCAGCCACA ACCCCAAATG 
GGTCACGTAA TGCTGCAGCA GCACCAGGCT CCTCCAGCAG CACAGGCACC TCCAGTTACC 
GAAATCGCCA ACAATCAAGT CCAAGCGGTG GCTGCTGCAC CTGCTCCGCC GCAAAACGAA 
CCGGGACCCG CGCCCGTTAA AGCTGAGGAG CCGGCTACGG CGGAACTGAT TAGCTTTGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722830.2
CDS	91..2373
Translation	MFRSSFDKNLENATSHLRLEPDWPSILLICDEINQKDVTPKNAFAAIKKKMNSPNPHSSCYSLLVLESIVKNCGAPVHEEVFTKENCEMFSSFLESTPHENVRQKMLELVQTWAYAFRSSDKYQAIKDTMTILKAKGHTFPELREADAMFTADTAPNWADGRVCHRCRVEFTFTNRKHHCRNCGQVFCGQCTAKQCPLPKYGIEKEVRVCDGCFAALQRPTSGSGGAKSGPRPADSELPAEYLNSTLAQQVQTPARKTEQELKEEEELQLALALSQSEAEQQKPKLQSLPPAAYRMQQRSPSPEAPPEPKEYHQQPEEATNPELAKYLNRSYWEQRKISESSSMASPSAPSPMPPTPQPQQIMPLQVKSADEVQIDEFAANMRTQVEIFVNRMKSNSSRGRSISNDSSVQTLFMTLTSLHSQQLSYIKEMDDKRMWYEQLQDKLTQIKDSRAALDQLRQEHVEKLRRIAEEQERQRQMQMAQKLDIMRKKKQEYLQYQRQLALQRIQEQEREMQLRQEQQKAQYLMGQSAPPFPYMPPSAVPQHGSPSHQLNNVYNPYAAGVPGYLPQGPAPAPNGHGQFQAIPPGMYNPAIQQPMPPNLQPGGLMQQPAPPGNPQMMPPMPENQFANNPAAILQLPQQHSIAQPPQIPFQPQPQQIPGQQPQQIPGQQPQQIPGQQPQQIPGQQPQQIPVQQPQPQPQMGHVMLQQHQAPPAAQAPPVTEIANNQVQAVAAAPAPPQNEPGPAPVKAEEPATAELISFD

Target ORF information:

RefSeq Version	NM_164496.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hepatocyte growth factor regulated tyrosine kinase substrate, transcript variant C (Hrs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164496.1

ORF Insert Sequence:

ATGTTTCGAT CAAGTTTCGA CAAAAACTTA GAAAATGCCA CCAGTCACTT GCGTCTGGAG 
CCCGACTGGC CATCAATTCT TCTGATTTGC GATGAAATCA ACCAGAAAGA TGTCACTCCC 
AAGAATGCCT TTGCCGCCAT CAAGAAGAAG ATGAACTCGC CCAACCCGCA CTCCTCCTGC 
TATTCCCTCC TGGTCCTCGA GAGCATCGTC AAGAACTGCG GCGCACCCGT TCACGAGGAG 
GTGTTCACCA AGGAGAACTG CGAGATGTTC AGCAGTTTCC TCGAGTCGAC GCCCCACGAG 
AACGTGCGCC AGAAGATGCT GGAGCTGGTG CAGACCTGGG CGTACGCCTT CCGCTCCTCG 
GACAAGTACC AGGCCATCAA GGACACCATG ACCATCCTGA AGGCGAAGGG GCACACTTTC 
CCCGAACTTC GGGAGGCAGA TGCCATGTTC ACGGCGGACA CGGCGCCCAA CTGGGCAGAT 
GGCAGGGTAT GCCATCGCTG TCGCGTGGAG TTCACCTTCA CCAACCGGAA GCACCACTGC 
CGGAACTGCG GCCAGGTCTT CTGTGGCCAA TGCACGGCCA AGCAGTGTCC GCTGCCCAAG 
TACGGCATCG AAAAGGAGGT GCGTGTGTGC GACGGCTGCT TCGCTGCCCT CCAGCGTCCG 
ACGAGCGGCA GTGGAGGCGC TAAGTCCGGA CCACGTCCGG CGGACAGCGA GCTACCGGCG 
GAGTATCTGA ACAGCACTTT GGCGCAGCAG GTGCAGACAC CAGCTCGCAA GACCGAGCAG 
GAACTCAAGG AGGAGGAGGA ACTGCAGCTG GCGCTGGCCC TCAGTCAATC GGAAGCGGAG 
CAGCAGAAGC CCAAGCTGCA ATCCCTGCCA CCCGCTGCCT ATCGCATGCA GCAAAGGTCT 
CCCAGTCCGG AGGCACCGCC AGAACCCAAG GAGTATCACC AGCAGCCGGA GGAGGCTACC 
AATCCGGAGC TGGCCAAGTA CTTGAACCGC AGCTACTGGG AGCAGCGCAA GATCAGCGAA 
TCGTCATCCA TGGCAAGTCC CTCGGCACCG AGCCCCATGC CACCAACGCC GCAGCCCCAG 
CAGATCATGC CCCTGCAGGT CAAGAGTGCG GATGAAGTGC AGATCGATGA GTTTGCCGCC 
AATATGCGCA CGCAGGTGGA GATCTTTGTT AACCGCATGA AGTCGAACTC GAGCCGCGGT 
CGCAGCATCT CCAACGACTC CTCTGTGCAG ACCCTCTTCA TGACCCTGAC CTCGTTGCAC 
TCGCAGCAGT TGTCGTACAT CAAGGAGATG GACGACAAGC GCATGTGGTA CGAGCAGCTG 
CAGGACAAGC TAACCCAGAT CAAGGACTCG CGTGCTGCGC TGGATCAGCT GCGTCAGGAG 
CATGTGGAGA AGCTGCGCAG GATCGCCGAG GAGCAGGAAC GTCAGCGACA GATGCAGATG 
GCCCAGAAAC TGGACATAAT GCGCAAGAAG AAGCAGGAGT ATCTGCAGTA TCAACGACAG 
CTAGCATTGC AGCGCATCCA GGAGCAGGAG CGCGAGATGC AGCTGCGCCA GGAGCAGCAG 
AAGGCGCAGT ATTTGATGGG CCAGTCGGCT CCACCTTTCC CCTACATGCC ACCATCGGCG 
GTGCCACAAC ATGGTTCGCC CTCGCATCAG CTCAACAACG TTTATAATCC CTATGCTGCC 
GGTGTGCCCG GCTATTTGCC TCAGGGTCCT GCACCGGCTC CCAACGGACA TGGCCAGTTT 
CAGGCCATTC CGCCGGGCAT GTACAATCCG GCAATACAGC AGCCGATGCC GCCGAATCTC 
CAGCCCGGCG GATTAATGCA ACAGCCCGCG CCACCTGGAA ATCCCCAGAT GATGCCGCCC 
ATGCCCGAAA ATCAGTTTGC CAACAATCCT GCTGCTATCC TCCAGCTGCC GCAGCAGCAT 
TCGATCGCCC AGCCTCCTCA AATACCATTC CAACCGCAGC CTCAACAGAT TCCTGGTCAG 
CAGCCTCAAC AGATTCCTGG TCAGCAGCCT CAACAGATTC CTGGTCAGCA GCCTCAACAG 
ATTCCTGGTC AGCAGCCACA GCAGATTCCT GTCCAGCAAC CTCAGCCACA ACCCCAAATG 
GGTCACGTAA TGCTGCAGCA GCACCAGGCT CCTCCAGCAG CACAGGCACC TCCAGTTACC 
GAAATCGCCA ACAATCAAGT CCAAGCGGTG GCTGCTGCAC CTGCTCCGCC GCAAAACGAA 
CCGGGACCCG CGCCCGTTAA AGCTGAGGAG CCGGCTACGG CGGAACTGAT TAGCTTTGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hrs ( NM_164497.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722831.1 Drosophila melanogaster hepatocyte growth factor regulated tyrosine kinase substrate, transcript variant A (Hrs), mRNA.

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CloneID	OFa13530
Clone ID Related Accession (Same CDS sequence)	NM_164497.1
Accession Version	NM_164497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	hepatocyte growth factor regulated tyrosine kinase substrate, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTCGG AGGAGGAACT TAGCATTGAG GAGGAATCCC TTGACACCAT GACCATCCTG 
AAGGCGAAGG GGCACACTTT CCCCGAACTT CGGGAGGCAG ATGCCATGTT CACGGCGGAC 
ACGGCGCCCA ACTGGGCAGA TGGCAGGGTA TGCCATCGCT GTCGCGTGGA GTTCACCTTC 
ACCAACCGGA AGCACCACTG CCGGAACTGC GGCCAGGTCT TCTGTGGCCA ATGCACGGCC 
AAGCAGTGTC CGCTGCCCAA GTACGGCATC GAAAAGGAGG TGCGTGTGTG CGACGGCTGC 
TTCGCTGCCC TCCAGCGTCC GACGAGCGGC AGTGGAGGCG CTAAGTCCGG ACCACGTCCG 
GCGGACAGCG AGCTACCGGC GGAGTATCTG AACAGCACTT TGGCGCAGCA GGTGCAGACA 
CCAGCTCGCA AGACCGAGCA GGAACTCAAG GAGGAGGAGG AACTGCAGCT GGCGCTGGCC 
CTCAGTCAAT CGGAAGCGGA GCAGCAGAAG CCCAAGCTGC AATCCCTGCC ACCCGCTGCC 
TATCGCATGC AGCAAAGGTC TCCCAGTCCG GAGGCACCGC CAGAACCCAA GGAGTATCAC 
CAGCAGCCGG AGGAGGCTAC CAATCCGGAG CTGGCCAAGT ACTTGAACCG CAGCTACTGG 
GAGCAGCGCA AGATCAGCGA ATCGTCATCC ATGGCAAGTC CCTCGGCACC GAGCCCCATG 
CCACCAACGC CGCAGCCCCA GCAGATCATG CCCCTGCAGG TCAAGAGTGC GGATGAAGTG 
CAGATCGATG AGTTTGCCGC CAATATGCGC ACGCAGGTGG AGATCTTTGT TAACCGCATG 
AAGTCGAACT CGAGCCGCGG TCGCAGCATC TCCAACGACT CCTCTGTGCA GACCCTCTTC 
ATGACCCTGA CCTCGTTGCA CTCGCAGCAG TTGTCGTACA TCAAGGAGAT GGACGACAAG 
CGCATGTGGT ACGAGCAGCT GCAGGACAAG CTAACCCAGA TCAAGGACTC GCGTGCTGCG 
CTGGATCAGC TGCGTCAGGA GCATGTGGAG AAGCTGCGCA GGATCGCCGA GGAGCAGGAA 
CGTCAGCGAC AGATGCAGAT GGCCCAGAAA CTGGACATAA TGCGCAAGAA GAAGCAGGAG 
TATCTGCAGT ATCAACGACA GCTAGCATTG CAGCGCATCC AGGAGCAGGA GCGCGAGATG 
CAGCTGCGCC AGGAGCAGCA GAAGGCGCAG TATTTGATGG GCCAGTCGGC TCCACCTTTC 
CCCTACATGC CACCATCGGC GGTGCCACAA CATGGTTCGC CCTCGCATCA GCTCAACAAC 
GTTTATAATC CCTATGCTGC CGGTGTGCCC GGCTATTTGC CTCAGGGTCC TGCACCGGCT 
CCCAACGGAC ATGGCCAGTT TCAGGCCATT CCGCCGGGCA TGTACAATCC GGCAATACAG 
CAGCCGATGC CGCCGAATCT CCAGCCCGGC GGATTAATGC AACAGCCCGC GCCACCTGGA 
AATCCCCAGA TGATGCCGCC CATGCCCGAA AATCAGTTTG CCAACAATCC TGCTGCTATC 
CTCCAGCTGC CGCAGCAGCA TTCGATCGCC CAGCCTCCTC AAATACCATT CCAACCGCAG 
CCTCAACAGA TTCCTGGTCA GCAGCCTCAA CAGATTCCTG GTCAGCAGCC TCAACAGATT 
CCTGGTCAGC AGCCTCAACA GATTCCTGGT CAGCAGCCAC AGCAGATTCC TGTCCAGCAA 
CCTCAGCCAC AACCCCAAAT GGGTCACGTA ATGCTGCAGC AGCACCAGGC TCCTCCAGCA 
GCACAGGCAC CTCCAGTTAC CGAAATCGCC AACAATCAAG TCCAAGCGGT GGCTGCTGCA 
CCTGCTCCGC CGCAAAACGA ACCGGGACCC GCGCCCGTTA AAGCTGAGGA GCCGGCTACG 
GCGGAACTGA TTAGCTTTGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722831.1
CDS	16..1959
Translation	MSSEEELSIEEESLDTMTILKAKGHTFPELREADAMFTADTAPNWADGRVCHRCRVEFTFTNRKHHCRNCGQVFCGQCTAKQCPLPKYGIEKEVRVCDGCFAALQRPTSGSGGAKSGPRPADSELPAEYLNSTLAQQVQTPARKTEQELKEEEELQLALALSQSEAEQQKPKLQSLPPAAYRMQQRSPSPEAPPEPKEYHQQPEEATNPELAKYLNRSYWEQRKISESSSMASPSAPSPMPPTPQPQQIMPLQVKSADEVQIDEFAANMRTQVEIFVNRMKSNSSRGRSISNDSSVQTLFMTLTSLHSQQLSYIKEMDDKRMWYEQLQDKLTQIKDSRAALDQLRQEHVEKLRRIAEEQERQRQMQMAQKLDIMRKKKQEYLQYQRQLALQRIQEQEREMQLRQEQQKAQYLMGQSAPPFPYMPPSAVPQHGSPSHQLNNVYNPYAAGVPGYLPQGPAPAPNGHGQFQAIPPGMYNPAIQQPMPPNLQPGGLMQQPAPPGNPQMMPPMPENQFANNPAAILQLPQQHSIAQPPQIPFQPQPQQIPGQQPQQIPGQQPQQIPGQQPQQIPGQQPQQIPVQQPQPQPQMGHVMLQQHQAPPAAQAPPVTEIANNQVQAVAAAPAPPQNEPGPAPVKAEEPATAELISFD

Target ORF information:

RefSeq Version	NM_164497.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hepatocyte growth factor regulated tyrosine kinase substrate, transcript variant A (Hrs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164497.1

ORF Insert Sequence:

ATGAGCTCGG AGGAGGAACT TAGCATTGAG GAGGAATCCC TTGACACCAT GACCATCCTG 
AAGGCGAAGG GGCACACTTT CCCCGAACTT CGGGAGGCAG ATGCCATGTT CACGGCGGAC 
ACGGCGCCCA ACTGGGCAGA TGGCAGGGTA TGCCATCGCT GTCGCGTGGA GTTCACCTTC 
ACCAACCGGA AGCACCACTG CCGGAACTGC GGCCAGGTCT TCTGTGGCCA ATGCACGGCC 
AAGCAGTGTC CGCTGCCCAA GTACGGCATC GAAAAGGAGG TGCGTGTGTG CGACGGCTGC 
TTCGCTGCCC TCCAGCGTCC GACGAGCGGC AGTGGAGGCG CTAAGTCCGG ACCACGTCCG 
GCGGACAGCG AGCTACCGGC GGAGTATCTG AACAGCACTT TGGCGCAGCA GGTGCAGACA 
CCAGCTCGCA AGACCGAGCA GGAACTCAAG GAGGAGGAGG AACTGCAGCT GGCGCTGGCC 
CTCAGTCAAT CGGAAGCGGA GCAGCAGAAG CCCAAGCTGC AATCCCTGCC ACCCGCTGCC 
TATCGCATGC AGCAAAGGTC TCCCAGTCCG GAGGCACCGC CAGAACCCAA GGAGTATCAC 
CAGCAGCCGG AGGAGGCTAC CAATCCGGAG CTGGCCAAGT ACTTGAACCG CAGCTACTGG 
GAGCAGCGCA AGATCAGCGA ATCGTCATCC ATGGCAAGTC CCTCGGCACC GAGCCCCATG 
CCACCAACGC CGCAGCCCCA GCAGATCATG CCCCTGCAGG TCAAGAGTGC GGATGAAGTG 
CAGATCGATG AGTTTGCCGC CAATATGCGC ACGCAGGTGG AGATCTTTGT TAACCGCATG 
AAGTCGAACT CGAGCCGCGG TCGCAGCATC TCCAACGACT CCTCTGTGCA GACCCTCTTC 
ATGACCCTGA CCTCGTTGCA CTCGCAGCAG TTGTCGTACA TCAAGGAGAT GGACGACAAG 
CGCATGTGGT ACGAGCAGCT GCAGGACAAG CTAACCCAGA TCAAGGACTC GCGTGCTGCG 
CTGGATCAGC TGCGTCAGGA GCATGTGGAG AAGCTGCGCA GGATCGCCGA GGAGCAGGAA 
CGTCAGCGAC AGATGCAGAT GGCCCAGAAA CTGGACATAA TGCGCAAGAA GAAGCAGGAG 
TATCTGCAGT ATCAACGACA GCTAGCATTG CAGCGCATCC AGGAGCAGGA GCGCGAGATG 
CAGCTGCGCC AGGAGCAGCA GAAGGCGCAG TATTTGATGG GCCAGTCGGC TCCACCTTTC 
CCCTACATGC CACCATCGGC GGTGCCACAA CATGGTTCGC CCTCGCATCA GCTCAACAAC 
GTTTATAATC CCTATGCTGC CGGTGTGCCC GGCTATTTGC CTCAGGGTCC TGCACCGGCT 
CCCAACGGAC ATGGCCAGTT TCAGGCCATT CCGCCGGGCA TGTACAATCC GGCAATACAG 
CAGCCGATGC CGCCGAATCT CCAGCCCGGC GGATTAATGC AACAGCCCGC GCCACCTGGA 
AATCCCCAGA TGATGCCGCC CATGCCCGAA AATCAGTTTG CCAACAATCC TGCTGCTATC 
CTCCAGCTGC CGCAGCAGCA TTCGATCGCC CAGCCTCCTC AAATACCATT CCAACCGCAG 
CCTCAACAGA TTCCTGGTCA GCAGCCTCAA CAGATTCCTG GTCAGCAGCC TCAACAGATT 
CCTGGTCAGC AGCCTCAACA GATTCCTGGT CAGCAGCCAC AGCAGATTCC TGTCCAGCAA 
CCTCAGCCAC AACCCCAAAT GGGTCACGTA ATGCTGCAGC AGCACCAGGC TCCTCCAGCA 
GCACAGGCAC CTCCAGTTAC CGAAATCGCC AACAATCAAG TCCAAGCGGT GGCTGCTGCA 
CCTGCTCCGC CGCAAAACGA ACCGGGACCC GCGCCCGTTA AAGCTGAGGA GCCGGCTACG 
GCGGAACTGA TTAGCTTTGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hrs cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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