Nle cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nle
Entrez Gene ID	33234
Full Name	Notchless
Synonyms	CG2863,Dmel\CG2863,anon-WO0026364,l(2)k13714
General protein information	Preferred Names Notchless Names CG2863 gene product from transcript CG2863-RA CG2863-PA Nle-PA notchless
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21E2-21E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057946.4	NP_477294.2	notchless

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NLE1	XP_001174396.2
Macaca mulatta (Rhesus monkey)	NLE1	XP_001113853.1
Mus musculus (house mouse)	Nle1	NP_663406.2
Neurospora crassa	NCU00794	XP_964950.1
Schizosaccharomyces pombe (fission yeast)	SPCC18.05c	NP_588384.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002221	XP_307962.4
Kluyveromyces lactis	KLLA0C08547g	XP_452581.1
Eremothecium gossypii	AGOS_AER263C	NP_985120.1
Rattus norvegicus (Norway rat)	Nle1	NP_001121006.1
Caenorhabditis elegans (roundworm)	W07E6.2	NP_493745.1
Homo sapiens (human)	NLE1	NP_060566.2
Canis lupus familiaris (dog)	NLE1	XP_005624858.1
Danio rerio (zebrafish)	nle1	NP_001018418.1
Drosophila melanogaster (fruit fly)	Nle	NP_477294.2
Oryza sativa (rice)	Os11g0594200	NP_001068204.1
Gallus gallus (chicken)	NLE1	XP_415857.3
Saccharomyces cerevisiae (baker's yeast)	RSA4	NP_009997.2
Oryza sativa (rice)	Os10g0104500	NP_001064020.1
Bos taurus (cattle)	NLE1	NP_001014887.1
Arabidopsis thaliana (thale cress)	AT5G52820	NP_200094.1
Xenopus tropicalis (tropical clawed frog)	nle1	NP_001072540.1
Magnaporthe oryzae (rice blast fungus)	MGG_08708	XP_003719269.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nle gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nle cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13368	NM_057946.4 Latest version!	Drosophila melanogaster notchless (Nle), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nle ( NM_057946.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477294.2 Drosophila melanogaster notchless (Nle), mRNA.

Price & Availability↑

CloneID	OFa13368
Clone ID Related Accession (Same CDS sequence)	NM_057946.4
Accession Version	NM_057946.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	notchless
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057946.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGGCCA AAAAACAGAA AATGCAGGAG ACGGACACGG AGCAAGAGGC CACGCCACAT 
ACGATACAGG CGCGCCTCGT TTCGGACACG GGCGAGGAAG CGGGCCCGCC AATCGACCTG 
CCGGCAGGAA TCACTACCCA GCAATTGGGT CTGATTTGCA ACGCGCTGCT GAAAAACGAG 
GAAGCCACTC CATATTTGTT TTTCGTGGGC GAGGATGAGA TCAAGAAGAG CCTGGAGGAC 
ACGTTGGACT TGGCGTCAGT GGACACCGAA AACGTGATCG ATATTGTGTA TCAGCCACAG 
GCGGTTTTCA AAGTGCGCCC AGTGACAAGA TGCACGAGTT CCATGCCGGG ACACGCCGAG 
GCTGTGGTTT CGCTGAATTT CAGCCCGGAT GGTGCTCATC TCGCCAGTGG AAGTGGCGAC 
ACCACAGTGC GATTGTGGGA TCTTAACACA GAGACACCGC ACTTCACCTG CACAGGTCAT 
AAGCAGTGGG TTCTGTGCGT ATCCTGGGCT CCGGATGGCA AACGGTTGGC CAGCGGTTGC 
AAAGCGGGCT CTATAATCAT CTGGGACCCG GAGACGGGTC AGCAGAAGGG GCGACCCTTG 
AGTGGGCACA AGAAACACAT CAACTGCCTC GCCTGGGAAC CGTATCATCG CGATCCGGAG 
TGCAGGAAAC TTGCTTCCGC CAGTGGAGAC GGGGACTGCC GGATTTGGGA CGTGAAATTG 
GGCCAGTGCC TTATGAACAT TGCCGGACAC ACAAATGCTG TGACAGCAGT GAGATGGGGT 
GGAGCGGGCC TTATTTATAC ATCCTCCAAA GATCGCACAG TGAAGATGTG GCGAGCAGCT 
GATGGAATCT TGTGCCGGAC GTTCTCTGGC CACGCTCACT GGGTAAACAA CATTGCGCTG 
AGCACCGACT ACGTCCTGCG CACTGGTCCA TTCCATCCGG TGAAGGATCG CTCCAAGAGC 
CACCTCAGTT TGAGCACTGA GGAATTGCAG GAATCTGCCT TGAAGCGCTA CCAGGCCGTG 
TGCCCTGACG AGGTGGAGTC GCTGGTTTCC TGTTCGGATG ACAACACCCT CTATCTGTGG 
CGGAACAACC AGAACAAGTG CGTTGAGCGC ATGACAGGGC ACCAGAACGT GGTCAACGAC 
GTGAAATATT CGCCGGATGT AAAGCTAATT GCGTCTGCTT CATTTGACAA GTCAGTGCGT 
CTGTGGCGAG CCAGCGATGG TCAGTACATG GCCACCTTCC GGGGTCATGT GCAGGCTGTT 
TACACGGTTG CCTGGTCCGC GGACTCCCGC TTGATTGTTT CCGGCAGCAA AGACTCAACT 
CTAAAAGTAT GGAGTGTGCA GACGAAGAAA CTGGCACAGG AGCTGCCTGG ACATGCGGAT 
GAGGTGTTCG GAGTGGACTG GGCGCCCGAT GGCTCTAGAG TTGCCTCTGG TGGCAAGGAC 
AAAGTTATAA AGCTATGGGC TTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477294.2
CDS	58..1524
Translation	MLAKKQKMQETDTEQEATPHTIQARLVSDTGEEAGPPIDLPAGITTQQLGLICNALLKNEEATPYLFFVGEDEIKKSLEDTLDLASVDTENVIDIVYQPQAVFKVRPVTRCTSSMPGHAEAVVSLNFSPDGAHLASGSGDTTVRLWDLNTETPHFTCTGHKQWVLCVSWAPDGKRLASGCKAGSIIIWDPETGQQKGRPLSGHKKHINCLAWEPYHRDPECRKLASASGDGDCRIWDVKLGQCLMNIAGHTNAVTAVRWGGAGLIYTSSKDRTVKMWRAADGILCRTFSGHAHWVNNIALSTDYVLRTGPFHPVKDRSKSHLSLSTEELQESALKRYQAVCPDEVESLVSCSDDNTLYLWRNNQNKCVERMTGHQNVVNDVKYSPDVKLIASASFDKSVRLWRASDGQYMATFRGHVQAVYTVAWSADSRLIVSGSKDSTLKVWSVQTKKLAQELPGHADEVFGVDWAPDGSRVASGGKDKVIKLWAY

Target ORF information:

RefSeq Version	NM_057946.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster notchless (Nle), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057946.4

ORF Insert Sequence:

ATGTTGGCCA AAAAACAGAA AATGCAGGAG ACGGACACGG AGCAAGAGGC CACGCCACAT 
ACGATACAGG CGCGCCTCGT TTCGGACACG GGCGAGGAAG CGGGCCCGCC AATCGACCTG 
CCGGCAGGAA TCACTACCCA GCAATTGGGT CTGATTTGCA ACGCGCTGCT GAAAAACGAG 
GAAGCCACTC CATATTTGTT TTTCGTGGGC GAGGATGAGA TCAAGAAGAG CCTGGAGGAC 
ACGTTGGACT TGGCGTCAGT GGACACCGAA AACGTGATCG ATATTGTGTA TCAGCCACAG 
GCGGTTTTCA AAGTGCGCCC AGTGACAAGA TGCACGAGTT CCATGCCGGG ACACGCCGAG 
GCTGTGGTTT CGCTGAATTT CAGCCCGGAT GGTGCTCATC TCGCCAGTGG AAGTGGCGAC 
ACCACAGTGC GATTGTGGGA TCTTAACACA GAGACACCGC ACTTCACCTG CACAGGTCAT 
AAGCAGTGGG TTCTGTGCGT ATCCTGGGCT CCGGATGGCA AACGGTTGGC CAGCGGTTGC 
AAAGCGGGCT CTATAATCAT CTGGGACCCG GAGACGGGTC AGCAGAAGGG GCGACCCTTG 
AGTGGGCACA AGAAACACAT CAACTGCCTC GCCTGGGAAC CGTATCATCG CGATCCGGAG 
TGCAGGAAAC TTGCTTCCGC CAGTGGAGAC GGGGACTGCC GGATTTGGGA CGTGAAATTG 
GGCCAGTGCC TTATGAACAT TGCCGGACAC ACAAATGCTG TGACAGCAGT GAGATGGGGT 
GGAGCGGGCC TTATTTATAC ATCCTCCAAA GATCGCACAG TGAAGATGTG GCGAGCAGCT 
GATGGAATCT TGTGCCGGAC GTTCTCTGGC CACGCTCACT GGGTAAACAA CATTGCGCTG 
AGCACCGACT ACGTCCTGCG CACTGGTCCA TTCCATCCGG TGAAGGATCG CTCCAAGAGC 
CACCTCAGTT TGAGCACTGA GGAATTGCAG GAATCTGCCT TGAAGCGCTA CCAGGCCGTG 
TGCCCTGACG AGGTGGAGTC GCTGGTTTCC TGTTCGGATG ACAACACCCT CTATCTGTGG 
CGGAACAACC AGAACAAGTG CGTTGAGCGC ATGACAGGGC ACCAGAACGT GGTCAACGAC 
GTGAAATATT CGCCGGATGT AAAGCTAATT GCGTCTGCTT CATTTGACAA GTCAGTGCGT 
CTGTGGCGAG CCAGCGATGG TCAGTACATG GCCACCTTCC GGGGTCATGT GCAGGCTGTT 
TACACGGTTG CCTGGTCCGC GGACTCCCGC TTGATTGTTT CCGGCAGCAA AGACTCAACT 
CTAAAAGTAT GGAGTGTGCA GACGAAGAAA CTGGCACAGG AGCTGCCTGG ACATGCGGAT 
GAGGTGTTCG GAGTGGACTG GGCGCCCGAT GGCTCTAGAG TTGCCTCTGG TGGCAAGGAC 
AAAGTTATAA AGCTATGGGC TTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nle cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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