eIF2alpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eIF2alpha
Entrez Gene ID	32617
Full Name	eukaryotic translation Initiation Factor 2alpha
Synonyms	CG9946,Dmel\CG9946,EIF-2alpha,Eif2alpha,IF2A_DROME,M(1)14C,M(1)19-153,M(1)8e3-10,deIF2alpha,eIF-2,eIF-2 a,eIF-2 alpha,eIF-2alphaeIF-2alpha,eIF2 alpha,eIF2-alpha,eIF2a,eIF2alpha,eiF-2alpha,elF2alpha,l(1)14Cf,l(1)19-153
General protein information	Preferred Names eukaryotic translation Initiation Factor 2alpha Names CG9946 gene product from transcript CG9946-RB CG9946-PA CG9946-PB eIF-2alpha-PA eIF-2alpha-PB eukaryotic initiation factor 2alpha eukaryotic translation initiation factor 2 eukaryotic translation initiation factor 2alpha minute (1) 14C
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14B13-14C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_132902.4	NP_573130.1	eukaryotic translation initiation factor 2 subunit alpha, isoform A
NM_001298400.1	NP_001285329.1	eukaryotic translation initiation factor 2 subunit alpha, isoform B

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_13200	XP_003712017.1
Bos taurus (cattle)	EIF2S1	NP_787007.1
Danio rerio (zebrafish)	eif2s1b	NP_571875.1
Saccharomyces cerevisiae (baker's yeast)	SUI2	NP_012540.3
Arabidopsis thaliana (thale cress)	EIF2 ALPHA	NP_196166.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011190	XP_309455.4
Canis lupus familiaris (dog)	EIF2S1	XP_005623592.1
Caenorhabditis elegans (roundworm)	Y37E3.10	NP_490930.1
Neurospora crassa	NCU08277	XP_961905.1
Mus musculus (house mouse)	Eif2s1	NP_080390.1
Rattus norvegicus (Norway rat)	Eif2s1	NP_062229.1
Drosophila melanogaster (fruit fly)	eIF-2alpha	NP_573130.1
Arabidopsis thaliana (thale cress)	AT2G40290	NP_565927.1
Xenopus tropicalis (tropical clawed frog)	eif2s1	NP_001005630.1
Schizosaccharomyces pombe (fission yeast)	tif211	NP_594081.1
Kluyveromyces lactis	KLLA0A11814g	XP_451514.1
Oryza sativa (rice)	Os07g0692800	NP_001060724.1
Eremothecium gossypii	AGOS_AGR320W	NP_986986.2
Homo sapiens (human)	EIF2S1	NP_004085.1
Macaca mulatta (Rhesus monkey)	EIF2S1	XP_001105924.1
Gallus gallus (chicken)	EIF2S1	NP_001006477.1
Oryza sativa (rice)	Os03g0296400	NP_001049829.1
Pan troglodytes (chimpanzee)	EIF2S1	XP_510016.1
Danio rerio (zebrafish)	eif2s1a	NP_955863.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eIF2alpha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eIF2alpha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01804	NM_132902.4 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa01804	NM_001298400.1 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eIF2alpha ( NM_132902.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573130.1 Drosophila melanogaster eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01804
Clone ID Related Accession (Same CDS sequence)	NM_132902.4 , NM_001298400.1
Accession Version	NM_132902.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 2 subunit alpha, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132902.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCTGA CGTCGCGCTT CTACAACGAG CGGTATCCGG AGATCGAGGA TGTCGTTATG 
GTGAACGTGC TGTCCATCGC CGAGATGGGC GCCTACGTTC ATCTGCTTGA GTACAACAAC 
ATCGAGGGCA TGATCCTGCT GTCGGAGCTG TCCCGCCGGC GCATCCGCTC CATCAACAAG 
CTGATTCGTG TCGGCAAGAC CGAACCGGTG GTGGTTATCC GTGTTGACAA GGAGAAGGGC 
TACATCGATC TGTCGAAGCG TCGCGTTTCG CCCGAAGATG TCGAGAAGTG CACCGAGCGC 
TTCGCTAAGG CCAAGGCCAT CAACTCACTG CTCCGCCATG TCGCCGACAT ACTCGGCTTC 
GAGGGCAACG AGAAGCTGGA AGATCTCTAC CAGAAGACCG CCTGGCACTT CGAGAAGAAG 
TACAACAACA AGACGGTCGC CTACGACATC TTCAAGCAAT CGGTCACCGA TCCCACCGTC 
TTCGATGAGT GCAACCTGGA ACCGGAGACC AAGGAGGTCC TGTTGAGCAA CATCAAGCGG 
AAGCTGGTCT CGCCCACTGT CAAGATCCGT GCGGACATCG AGTGCTCCTG CTACGGTTAC 
GAGGGCATCG ACGCTGTCAA GGCATCGCTC ACCAAGGGCC TGGAGCTGAG CACCGAGGAG 
CTGCCCATTC GCATCAACCT GATAGCACCG CCACTCTATG TAATGACCAC ATCCACTACC 
AAGAAGACAG ACGGTTTAAA GGCTCTGGAG GTGGCCATTG AGCACATTCG CGCCAAGACC 
AGCGAGTACG ATGGAGAGTT CAAGGTGATC ATGGCACCCA AACTGGTTAC GGCCATCGAC 
GAGGCGGATC TGGCCAGACG CCTGGAGCGC GCTGAGGCCG AGAACGCCCA GGTGGCTGGC 
GACGATGACG AGGAGGATGG CGCCGACCAG GAGGGCATGC AGTTCGATCC AGAGAAGGAG 
TTCAACCACA AGGGATCGGG GGCGGGTCGT GCGAACGAGG AGGATGAGGA GGAGGAAGAG 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573130.1
CDS	115..1140
Translation	MALTSRFYNERYPEIEDVVMVNVLSIAEMGAYVHLLEYNNIEGMILLSELSRRRIRSINKLIRVGKTEPVVVIRVDKEKGYIDLSKRRVSPEDVEKCTERFAKAKAINSLLRHVADILGFEGNEKLEDLYQKTAWHFEKKYNNKTVAYDIFKQSVTDPTVFDECNLEPETKEVLLSNIKRKLVSPTVKIRADIECSCYGYEGIDAVKASLTKGLELSTEELPIRINLIAPPLYVMTTSTTKKTDGLKALEVAIEHIRAKTSEYDGEFKVIMAPKLVTAIDEADLARRLERAEAENAQVAGDDDEEDGADQEGMQFDPEKEFNHKGSGAGRANEEDEEEEED

Target ORF information:

RefSeq Version	NM_132902.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132902.4

ORF Insert Sequence:

ATGGCCCTGA CGTCGCGCTT CTACAACGAG CGGTATCCGG AGATCGAGGA TGTCGTTATG 
GTGAACGTGC TGTCCATCGC CGAGATGGGC GCCTACGTTC ATCTGCTTGA GTACAACAAC 
ATCGAGGGCA TGATCCTGCT GTCGGAGCTG TCCCGCCGGC GCATCCGCTC CATCAACAAG 
CTGATTCGTG TCGGCAAGAC CGAACCGGTG GTGGTTATCC GTGTTGACAA GGAGAAGGGC 
TACATCGATC TGTCGAAGCG TCGCGTTTCG CCCGAAGATG TCGAGAAGTG CACCGAGCGC 
TTCGCTAAGG CCAAGGCCAT CAACTCACTG CTCCGCCATG TCGCCGACAT ACTCGGCTTC 
GAGGGCAACG AGAAGCTGGA AGATCTCTAC CAGAAGACCG CCTGGCACTT CGAGAAGAAG 
TACAACAACA AGACGGTCGC CTACGACATC TTCAAGCAAT CGGTCACCGA TCCCACCGTC 
TTCGATGAGT GCAACCTGGA ACCGGAGACC AAGGAGGTCC TGTTGAGCAA CATCAAGCGG 
AAGCTGGTCT CGCCCACTGT CAAGATCCGT GCGGACATCG AGTGCTCCTG CTACGGTTAC 
GAGGGCATCG ACGCTGTCAA GGCATCGCTC ACCAAGGGCC TGGAGCTGAG CACCGAGGAG 
CTGCCCATTC GCATCAACCT GATAGCACCG CCACTCTATG TAATGACCAC ATCCACTACC 
AAGAAGACAG ACGGTTTAAA GGCTCTGGAG GTGGCCATTG AGCACATTCG CGCCAAGACC 
AGCGAGTACG ATGGAGAGTT CAAGGTGATC ATGGCACCCA AACTGGTTAC GGCCATCGAC 
GAGGCGGATC TGGCCAGACG CCTGGAGCGC GCTGAGGCCG AGAACGCCCA GGTGGCTGGC 
GACGATGACG AGGAGGATGG CGCCGACCAG GAGGGCATGC AGTTCGATCC AGAGAAGGAG 
TTCAACCACA AGGGATCGGG GGCGGGTCGT GCGAACGAGG AGGATGAGGA GGAGGAAGAG 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF2alpha ( NM_001298400.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285329.1 Drosophila melanogaster eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01804
Clone ID Related Accession (Same CDS sequence)	NM_132902.4 , NM_001298400.1
Accession Version	NM_001298400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 2 subunit alpha, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCTGA CGTCGCGCTT CTACAACGAG CGGTATCCGG AGATCGAGGA TGTCGTTATG 
GTGAACGTGC TGTCCATCGC CGAGATGGGC GCCTACGTTC ATCTGCTTGA GTACAACAAC 
ATCGAGGGCA TGATCCTGCT GTCGGAGCTG TCCCGCCGGC GCATCCGCTC CATCAACAAG 
CTGATTCGTG TCGGCAAGAC CGAACCGGTG GTGGTTATCC GTGTTGACAA GGAGAAGGGC 
TACATCGATC TGTCGAAGCG TCGCGTTTCG CCCGAAGATG TCGAGAAGTG CACCGAGCGC 
TTCGCTAAGG CCAAGGCCAT CAACTCACTG CTCCGCCATG TCGCCGACAT ACTCGGCTTC 
GAGGGCAACG AGAAGCTGGA AGATCTCTAC CAGAAGACCG CCTGGCACTT CGAGAAGAAG 
TACAACAACA AGACGGTCGC CTACGACATC TTCAAGCAAT CGGTCACCGA TCCCACCGTC 
TTCGATGAGT GCAACCTGGA ACCGGAGACC AAGGAGGTCC TGTTGAGCAA CATCAAGCGG 
AAGCTGGTCT CGCCCACTGT CAAGATCCGT GCGGACATCG AGTGCTCCTG CTACGGTTAC 
GAGGGCATCG ACGCTGTCAA GGCATCGCTC ACCAAGGGCC TGGAGCTGAG CACCGAGGAG 
CTGCCCATTC GCATCAACCT GATAGCACCG CCACTCTATG TAATGACCAC ATCCACTACC 
AAGAAGACAG ACGGTTTAAA GGCTCTGGAG GTGGCCATTG AGCACATTCG CGCCAAGACC 
AGCGAGTACG ATGGAGAGTT CAAGGTGATC ATGGCACCCA AACTGGTTAC GGCCATCGAC 
GAGGCGGATC TGGCCAGACG CCTGGAGCGC GCTGAGGCCG AGAACGCCCA GGTGGCTGGC 
GACGATGACG AGGAGGATGG CGCCGACCAG GAGGGCATGC AGTTCGATCC AGAGAAGGAG 
TTCAACCACA AGGGATCGGG GGCGGGTCGT GCGAACGAGG AGGATGAGGA GGAGGAAGAG 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285329.1
CDS	115..1140
Translation	MALTSRFYNERYPEIEDVVMVNVLSIAEMGAYVHLLEYNNIEGMILLSELSRRRIRSINKLIRVGKTEPVVVIRVDKEKGYIDLSKRRVSPEDVEKCTERFAKAKAINSLLRHVADILGFEGNEKLEDLYQKTAWHFEKKYNNKTVAYDIFKQSVTDPTVFDECNLEPETKEVLLSNIKRKLVSPTVKIRADIECSCYGYEGIDAVKASLTKGLELSTEELPIRINLIAPPLYVMTTSTTKKTDGLKALEVAIEHIRAKTSEYDGEFKVIMAPKLVTAIDEADLARRLERAEAENAQVAGDDDEEDGADQEGMQFDPEKEFNHKGSGAGRANEEDEEEEED

Target ORF information:

RefSeq Version	NM_001298400.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298400.1

ORF Insert Sequence:

ATGGCCCTGA CGTCGCGCTT CTACAACGAG CGGTATCCGG AGATCGAGGA TGTCGTTATG 
GTGAACGTGC TGTCCATCGC CGAGATGGGC GCCTACGTTC ATCTGCTTGA GTACAACAAC 
ATCGAGGGCA TGATCCTGCT GTCGGAGCTG TCCCGCCGGC GCATCCGCTC CATCAACAAG 
CTGATTCGTG TCGGCAAGAC CGAACCGGTG GTGGTTATCC GTGTTGACAA GGAGAAGGGC 
TACATCGATC TGTCGAAGCG TCGCGTTTCG CCCGAAGATG TCGAGAAGTG CACCGAGCGC 
TTCGCTAAGG CCAAGGCCAT CAACTCACTG CTCCGCCATG TCGCCGACAT ACTCGGCTTC 
GAGGGCAACG AGAAGCTGGA AGATCTCTAC CAGAAGACCG CCTGGCACTT CGAGAAGAAG 
TACAACAACA AGACGGTCGC CTACGACATC TTCAAGCAAT CGGTCACCGA TCCCACCGTC 
TTCGATGAGT GCAACCTGGA ACCGGAGACC AAGGAGGTCC TGTTGAGCAA CATCAAGCGG 
AAGCTGGTCT CGCCCACTGT CAAGATCCGT GCGGACATCG AGTGCTCCTG CTACGGTTAC 
GAGGGCATCG ACGCTGTCAA GGCATCGCTC ACCAAGGGCC TGGAGCTGAG CACCGAGGAG 
CTGCCCATTC GCATCAACCT GATAGCACCG CCACTCTATG TAATGACCAC ATCCACTACC 
AAGAAGACAG ACGGTTTAAA GGCTCTGGAG GTGGCCATTG AGCACATTCG CGCCAAGACC 
AGCGAGTACG ATGGAGAGTT CAAGGTGATC ATGGCACCCA AACTGGTTAC GGCCATCGAC 
GAGGCGGATC TGGCCAGACG CCTGGAGCGC GCTGAGGCCG AGAACGCCCA GGTGGCTGGC 
GACGATGACG AGGAGGATGG CGCCGACCAG GAGGGCATGC AGTTCGATCC AGAGAAGGAG 
TTCAACCACA AGGGATCGGG GGCGGGTCGT GCGAACGAGG AGGATGAGGA GGAGGAAGAG 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eIF2alpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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