CtsB1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CtsB1
Entrez Gene ID	32341
Full Name	Cathepsin B1
Synonyms	CB,CG-10992,CG10992,CTSB1,Dmel\CG10992,anon-EST:ParkEST132
General protein information	Preferred Names Cathepsin B1 Names CG10992 gene product from transcript CG10992-RC CG10992-PA CG10992-PC CtsB1-PA CtsB1-PC cathepsin B cathepsin B1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 12C4-12C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_132692.3	NP_572920.1	cathepsin B1, isoform A
NM_001272607.2	NP_001259536.2	cathepsin B1, isoform C

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CTSB	NP_776456.1
Gallus gallus (chicken)	CTSB	XP_004935955.1
Danio rerio (zebrafish)	ctsba	NP_998501.1
Oryza sativa (rice)	Os05g0310500	NP_001174338.1
Homo sapiens (human)	CTSB	NP_680090.1
Canis lupus familiaris (dog)	CTSB	XP_543203.3
Rattus norvegicus (Norway rat)	Ctsb	NP_072119.2
Arabidopsis thaliana (thale cress)	AT1G02300	NP_563647.1
Pan troglodytes (chimpanzee)	CTSB	XP_003317965.1
Xenopus tropicalis (tropical clawed frog)	ctsb	NP_989225.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004533	XP_313835.5
Drosophila melanogaster (fruit fly)	CtsB1	NP_572920.1
Arabidopsis thaliana (thale cress)	AT1G02305	NP_563648.1
Mus musculus (house mouse)	Ctsb	NP_031824.1
Arabidopsis thaliana (thale cress)	AT4G01610	NP_567215.1
Macaca mulatta (Rhesus monkey)	CTSB	XP_002805301.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CtsB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CtsB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01545	NM_001272607.2 Latest version!	Drosophila melanogaster cathepsin B1, transcript variant C (CtsB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa01545	NM_132692.3 Latest version!	Drosophila melanogaster cathepsin B1, transcript variant A (CtsB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CtsB1 ( NM_132692.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572920.1 Drosophila melanogaster cathepsin B1, transcript variant A (CtsB1), mRNA.

Price & Availability↑

CloneID	OFa01545
Clone ID Related Accession (Same CDS sequence)	NM_132692.3 , NM_001272607.2
Accession Version	NM_132692.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	cathepsin B1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132692.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTAT TGCTCCTGGT GGCCACCGCG GCCTCCGTGG CGGCACTCAC GTCCGGTGAG 
CCGTCACTGC TCTCGGATGA GTTCATCGAG GTGGTGCGCA GTAAGGCCAA AACCTGGACG 
GTGGGTCGCA ATTTCGATGC ATCCGTAACG GAGGGACATA TTCGCCGCCT GATGGGCGTC 
CATCCGGATG CGCACAAGTT CGCACTGCCC GACAAGCGTG AAGTTCTTGG CGATCTATAC 
GTGAATAGCG TTGACGAACT TCCCGAAGAG TTCGATTCCC GCAAGCAATG GCCAAACTGC 
CCAACCATTG GCGAGATTCG CGACCAGGGT TCATGCGGCT CCTGCTGGGC CTTTGGTGCC 
GTGGAAGCCA TGTCCGATCG CGTGTGCATC CATTCCGGCG GCAAGGTGAA TTTCCACTTT 
TCGGCCGATG ATCTCGTCTC CTGCTGCCAC ACCTGCGGAT TTGGCTGCAA TGGCGGTTTC 
CCCGGCGCCG CCTGGAGCTA CTGGACGCGC AAGGGAATCG TCAGCGGAGG ACCCTACGGA 
TCTAATCAGG GCTGCCGTCC TTACGAGATT TCTCCCTGTG AGCACCATGT GAATGGAACC 
CGACCACCAT GTGCCCATGG CGGTAGAACG CCCAAGTGTT CGCACGTCTG CCAGAGCGGT 
TACACCGTCG ATTATGCCAA GGACAAGCAC TTCGGCTCCA AGTCGTACTC GGTGAGGCGT 
AATGTGCGTG AAATTCAGGA GGAGATCATG ACCAATGGAC CGGTTGAGGG CGCCTTCACC 
GTTTATGAGG ATCTCATTCT GTACAAGGAC GGTGTCTATC AGCACGAGCA TGGCAAGGAG 
CTGGGCGGTC ATGCCATTCG CATTTTGGGC TGGGGCGTTT GGGGCGAGGA GAAGATTCCG 
TACTGGCTAA TTGGTAACTC GTGGAACACC GATTGGGGCG ATCATGGTTT CTTCCGCATC 
CTGCGTGGCC AGGATCATTG CGGCATCGAG AGCTCCATTT CGGCGGGTCT GCCCAAGCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572920.1
CDS	242..1264
Translation	MNLLLLVATAASVAALTSGEPSLLSDEFIEVVRSKAKTWTVGRNFDASVTEGHIRRLMGVHPDAHKFALPDKREVLGDLYVNSVDELPEEFDSRKQWPNCPTIGEIRDQGSCGSCWAFGAVEAMSDRVCIHSGGKVNFHFSADDLVSCCHTCGFGCNGGFPGAAWSYWTRKGIVSGGPYGSNQGCRPYEISPCEHHVNGTRPPCAHGGRTPKCSHVCQSGYTVDYAKDKHFGSKSYSVRRNVREIQEEIMTNGPVEGAFTVYEDLILYKDGVYQHEHGKELGGHAIRILGWGVWGEEKIPYWLIGNSWNTDWGDHGFFRILRGQDHCGIESSISAGLPKL

Target ORF information:

RefSeq Version	NM_132692.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cathepsin B1, transcript variant A (CtsB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132692.3

ORF Insert Sequence:

ATGAATCTAT TGCTCCTGGT GGCCACCGCG GCCTCCGTGG CGGCACTCAC GTCCGGTGAG 
CCGTCACTGC TCTCGGATGA GTTCATCGAG GTGGTGCGCA GTAAGGCCAA AACCTGGACG 
GTGGGTCGCA ATTTCGATGC ATCCGTAACG GAGGGACATA TTCGCCGCCT GATGGGCGTC 
CATCCGGATG CGCACAAGTT CGCACTGCCC GACAAGCGTG AAGTTCTTGG CGATCTATAC 
GTGAATAGCG TTGACGAACT TCCCGAAGAG TTCGATTCCC GCAAGCAATG GCCAAACTGC 
CCAACCATTG GCGAGATTCG CGACCAGGGT TCATGCGGCT CCTGCTGGGC CTTTGGTGCC 
GTGGAAGCCA TGTCCGATCG CGTGTGCATC CATTCCGGCG GCAAGGTGAA TTTCCACTTT 
TCGGCCGATG ATCTCGTCTC CTGCTGCCAC ACCTGCGGAT TTGGCTGCAA TGGCGGTTTC 
CCCGGCGCCG CCTGGAGCTA CTGGACGCGC AAGGGAATCG TCAGCGGAGG ACCCTACGGA 
TCTAATCAGG GCTGCCGTCC TTACGAGATT TCTCCCTGTG AGCACCATGT GAATGGAACC 
CGACCACCAT GTGCCCATGG CGGTAGAACG CCCAAGTGTT CGCACGTCTG CCAGAGCGGT 
TACACCGTCG ATTATGCCAA GGACAAGCAC TTCGGCTCCA AGTCGTACTC GGTGAGGCGT 
AATGTGCGTG AAATTCAGGA GGAGATCATG ACCAATGGAC CGGTTGAGGG CGCCTTCACC 
GTTTATGAGG ATCTCATTCT GTACAAGGAC GGTGTCTATC AGCACGAGCA TGGCAAGGAG 
CTGGGCGGTC ATGCCATTCG CATTTTGGGC TGGGGCGTTT GGGGCGAGGA GAAGATTCCG 
TACTGGCTAA TTGGTAACTC GTGGAACACC GATTGGGGCG ATCATGGTTT CTTCCGCATC 
CTGCGTGGCC AGGATCATTG CGGCATCGAG AGCTCCATTT CGGCGGGTCT GCCCAAGCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CtsB1 ( NM_001272607.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259536.2 Drosophila melanogaster cathepsin B1, transcript variant C (CtsB1), mRNA.

Price & Availability↑

CloneID	OFa01545
Clone ID Related Accession (Same CDS sequence)	NM_132692.3 , NM_001272607.2
Accession Version	NM_001272607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	cathepsin B1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272607.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTAT TGCTCCTGGT GGCCACCGCG GCCTCCGTGG CGGCACTCAC GTCCGGTGAG 
CCGTCACTGC TCTCGGATGA GTTCATCGAG GTGGTGCGCA GTAAGGCCAA AACCTGGACG 
GTGGGTCGCA ATTTCGATGC ATCCGTAACG GAGGGACATA TTCGCCGCCT GATGGGCGTC 
CATCCGGATG CGCACAAGTT CGCACTGCCC GACAAGCGTG AAGTTCTTGG CGATCTATAC 
GTGAATAGCG TTGACGAACT TCCCGAAGAG TTCGATTCCC GCAAGCAATG GCCAAACTGC 
CCAACCATTG GCGAGATTCG CGACCAGGGT TCATGCGGCT CCTGCTGGGC CTTTGGTGCC 
GTGGAAGCCA TGTCCGATCG CGTGTGCATC CATTCCGGCG GCAAGGTGAA TTTCCACTTT 
TCGGCCGATG ATCTCGTCTC CTGCTGCCAC ACCTGCGGAT TTGGCTGCAA TGGCGGTTTC 
CCCGGCGCCG CCTGGAGCTA CTGGACGCGC AAGGGAATCG TCAGCGGAGG ACCCTACGGA 
TCTAATCAGG GCTGCCGTCC TTACGAGATT TCTCCCTGTG AGCACCATGT GAATGGAACC 
CGACCACCAT GTGCCCATGG CGGTAGAACG CCCAAGTGTT CGCACGTCTG CCAGAGCGGT 
TACACCGTCG ATTATGCCAA GGACAAGCAC TTCGGCTCCA AGTCGTACTC GGTGAGGCGT 
AATGTGCGTG AAATTCAGGA GGAGATCATG ACCAATGGAC CGGTTGAGGG CGCCTTCACC 
GTTTATGAGG ATCTCATTCT GTACAAGGAC GGTGTCTATC AGCACGAGCA TGGCAAGGAG 
CTGGGCGGTC ATGCCATTCG CATTTTGGGC TGGGGCGTTT GGGGCGAGGA GAAGATTCCG 
TACTGGCTAA TTGGTAACTC GTGGAACACC GATTGGGGCG ATCATGGTTT CTTCCGCATC 
CTGCGTGGCC AGGATCATTG CGGCATCGAG AGCTCCATTT CGGCGGGTCT GCCCAAGCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259536.2
CDS	242..1264
Translation	MNLLLLVATAASVAALTSGEPSLLSDEFIEVVRSKAKTWTVGRNFDASVTEGHIRRLMGVHPDAHKFALPDKREVLGDLYVNSVDELPEEFDSRKQWPNCPTIGEIRDQGSCGSCWAFGAVEAMSDRVCIHSGGKVNFHFSADDLVSCCHTCGFGCNGGFPGAAWSYWTRKGIVSGGPYGSNQGCRPYEISPCEHHVNGTRPPCAHGGRTPKCSHVCQSGYTVDYAKDKHFGSKSYSVRRNVREIQEEIMTNGPVEGAFTVYEDLILYKDGVYQHEHGKELGGHAIRILGWGVWGEEKIPYWLIGNSWNTDWGDHGFFRILRGQDHCGIESSISAGLPKL

Target ORF information:

RefSeq Version	NM_001272607.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cathepsin B1, transcript variant C (CtsB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272607.2

ORF Insert Sequence:

ATGAATCTAT TGCTCCTGGT GGCCACCGCG GCCTCCGTGG CGGCACTCAC GTCCGGTGAG 
CCGTCACTGC TCTCGGATGA GTTCATCGAG GTGGTGCGCA GTAAGGCCAA AACCTGGACG 
GTGGGTCGCA ATTTCGATGC ATCCGTAACG GAGGGACATA TTCGCCGCCT GATGGGCGTC 
CATCCGGATG CGCACAAGTT CGCACTGCCC GACAAGCGTG AAGTTCTTGG CGATCTATAC 
GTGAATAGCG TTGACGAACT TCCCGAAGAG TTCGATTCCC GCAAGCAATG GCCAAACTGC 
CCAACCATTG GCGAGATTCG CGACCAGGGT TCATGCGGCT CCTGCTGGGC CTTTGGTGCC 
GTGGAAGCCA TGTCCGATCG CGTGTGCATC CATTCCGGCG GCAAGGTGAA TTTCCACTTT 
TCGGCCGATG ATCTCGTCTC CTGCTGCCAC ACCTGCGGAT TTGGCTGCAA TGGCGGTTTC 
CCCGGCGCCG CCTGGAGCTA CTGGACGCGC AAGGGAATCG TCAGCGGAGG ACCCTACGGA 
TCTAATCAGG GCTGCCGTCC TTACGAGATT TCTCCCTGTG AGCACCATGT GAATGGAACC 
CGACCACCAT GTGCCCATGG CGGTAGAACG CCCAAGTGTT CGCACGTCTG CCAGAGCGGT 
TACACCGTCG ATTATGCCAA GGACAAGCAC TTCGGCTCCA AGTCGTACTC GGTGAGGCGT 
AATGTGCGTG AAATTCAGGA GGAGATCATG ACCAATGGAC CGGTTGAGGG CGCCTTCACC 
GTTTATGAGG ATCTCATTCT GTACAAGGAC GGTGTCTATC AGCACGAGCA TGGCAAGGAG 
CTGGGCGGTC ATGCCATTCG CATTTTGGGC TGGGGCGTTT GGGGCGAGGA GAAGATTCCG 
TACTGGCTAA TTGGTAACTC GTGGAACACC GATTGGGGCG ATCATGGTTT CTTCCGCATC 
CTGCGTGGCC AGGATCATTG CGGCATCGAG AGCTCCATTT CGGCGGGTCT GCCCAAGCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CtsB1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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