Spx cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spx
Entrez Gene ID	31558
Full Name	Spliceosomal protein on the X
Synonyms	CG3780,Dmel\CG3780,SPX,spx
General protein information	Preferred Names Spliceosomal protein on the X Names CG3780 gene product from transcript CG3780-RA CG3780-PA Spx-PA spliceosomal protein on the X
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5E3-5E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_078503.5	NP_511058.1	spliceosomal protein on the X

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spx gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spx cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03117	NM_078503.5 Latest version!	Drosophila melanogaster spliceosomal protein on the X (Spx), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spx ( NM_078503.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511058.1 Drosophila melanogaster spliceosomal protein on the X (Spx), mRNA.

Price & Availability↑

CloneID	OFa03117
Clone ID Related Accession (Same CDS sequence)	NM_078503.5
Accession Version	NM_078503.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	spliceosomal protein on the X
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078503.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCAG GTCCAATTGC TGAACGCAAC CAAGATGCCA CCATCTATGC CGGCGGTCTT 
GACGACAAGG TGTCGGAAAC GCTGCTTTGG GAGCTGTTTG TCCAGGCAGG ACCAGTGGTG 
AACGTACACA TGCCCAAGGA TCGTGTCACC CAAATGCACC AGGGCTATGG ATTCGTCGAG 
TTCCTCAGCG AGGAAGACGC CGACTATGGA ATAAAGATCA TGAACATGAT CAAGCTTTAC 
GGCAAGCCCA TTCGCGTGAA CAAGGCGTCG GCGCATCAGA AGAACCTGGA CGTCGGCGCC 
AACATCTTCA TTGGCAACCT AGACGTGGAA GTGGACGAGA AGCTGCTGTA CGATACGTTC 
TCCGCCTTCG GCGTTATACT GCAGACGCCG AAGATCATGC GAGACCCAGA GACGGGCAAG 
TCTAAGAGCT TTGCGTTCAT CAACTTTGCC AGCTTCGAGG CCAGCGATGC GGCCATGGAC 
GCCATGAACG GCCAGTACCT GTGCAACCGT CCCATTTCCG TGTCCTATGC CTTCAAGAAG 
GATCACAAGG GCGAGCGTCA CGGTTCGGCT GCGGAGCGCC TGTTGGCCGC CCAGAATCCA 
TCGACCCATG CAGACCGGCC GCATCAATTG TTCGCCGATG CACCGGTGCA AACCATGATG 
CCCCAGATGC CGGGCCAGAT CCCGGCCCAG ATGCCGGGCC AGATGATGCC GCCACCCATG 
ATGGCGCCGC CGCCGCCGGT GGTGCCCGTT TCGAATAACA ATATGGGCAT GCTAGCACCG 
CCGCCGCCCG TTCCCCAGCC TGCACCGTTC CCAGCTACGA TACCGCCGCC TCCGTTGCCA 
CCGATGACCG GCGGACAGCC GCCGCTGCCG CCAGCAATGG GCATTCCGCC GCCGCCTCGC 
ATGATGCAAC CAAATGCATG GGCACCGCCC GGCATGCCTG CTCCTCCGCC CAGACCACCG 
CCCACCAACT GGCGTCCGCC GCCCGTGCCC TTCCCACCCA CGCCCTACGC ACGGCCCTAT 
CAACCCGACG GCTACCAGTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511058.1
CDS	90..1133
Translation	MAAGPIAERNQDATIYAGGLDDKVSETLLWELFVQAGPVVNVHMPKDRVTQMHQGYGFVEFLSEEDADYGIKIMNMIKLYGKPIRVNKASAHQKNLDVGANIFIGNLDVEVDEKLLYDTFSAFGVILQTPKIMRDPETGKSKSFAFINFASFEASDAAMDAMNGQYLCNRPISVSYAFKKDHKGERHGSAAERLLAAQNPSTHADRPHQLFADAPVQTMMPQMPGQIPAQMPGQMMPPPMMAPPPPVVPVSNNNMGMLAPPPPVPQPAPFPATIPPPPLPPMTGGQPPLPPAMGIPPPPRMMQPNAWAPPGMPAPPPRPPPTNWRPPPVPFPPTPYARPYQPDGYQY

Target ORF information:

RefSeq Version	NM_078503.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spliceosomal protein on the X (Spx), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078503.5

ORF Insert Sequence:

ATGGCGGCAG GTCCAATTGC TGAACGCAAC CAAGATGCCA CCATCTATGC CGGCGGTCTT 
GACGACAAGG TGTCGGAAAC GCTGCTTTGG GAGCTGTTTG TCCAGGCAGG ACCAGTGGTG 
AACGTACACA TGCCCAAGGA TCGTGTCACC CAAATGCACC AGGGCTATGG ATTCGTCGAG 
TTCCTCAGCG AGGAAGACGC CGACTATGGA ATAAAGATCA TGAACATGAT CAAGCTTTAC 
GGCAAGCCCA TTCGCGTGAA CAAGGCGTCG GCGCATCAGA AGAACCTGGA CGTCGGCGCC 
AACATCTTCA TTGGCAACCT AGACGTGGAA GTGGACGAGA AGCTGCTGTA CGATACGTTC 
TCCGCCTTCG GCGTTATACT GCAGACGCCG AAGATCATGC GAGACCCAGA GACGGGCAAG 
TCTAAGAGCT TTGCGTTCAT CAACTTTGCC AGCTTCGAGG CCAGCGATGC GGCCATGGAC 
GCCATGAACG GCCAGTACCT GTGCAACCGT CCCATTTCCG TGTCCTATGC CTTCAAGAAG 
GATCACAAGG GCGAGCGTCA CGGTTCGGCT GCGGAGCGCC TGTTGGCCGC CCAGAATCCA 
TCGACCCATG CAGACCGGCC GCATCAATTG TTCGCCGATG CACCGGTGCA AACCATGATG 
CCCCAGATGC CGGGCCAGAT CCCGGCCCAG ATGCCGGGCC AGATGATGCC GCCACCCATG 
ATGGCGCCGC CGCCGCCGGT GGTGCCCGTT TCGAATAACA ATATGGGCAT GCTAGCACCG 
CCGCCGCCCG TTCCCCAGCC TGCACCGTTC CCAGCTACGA TACCGCCGCC TCCGTTGCCA 
CCGATGACCG GCGGACAGCC GCCGCTGCCG CCAGCAATGG GCATTCCGCC GCCGCCTCGC 
ATGATGCAAC CAAATGCATG GGCACCGCCC GGCATGCCTG CTCCTCCGCC CAGACCACCG 
CCCACCAACT GGCGTCCGCC GCCCGTGCCC TTCCCACCCA CGCCCTACGC ACGGCCCTAT 
CAACCCGACG GCTACCAGTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Spx cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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