Mcm3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mcm3
Entrez Gene ID	31449
Full Name	Minichromosome maintenance 3
Synonyms	CG4206,DmMCM3,DmMcm3,DmeMCM3,Dmel\CG4206,MCM3,McM3,Mcp-PCR2,PCR2,dMCM3,dMcm3
General protein information	Preferred Names Minichromosome maintenance 3 Names CG4206 gene product from transcript CG4206-RA CG4206-PA CG4206-PB Mcm3-PA Mcm3-PB minichromosome maintenance 3 minichromosome maintenance-PCR2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 4F5-4F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297955.1	NP_001284884.1	minichromosome maintenance 3, isoform B
NM_078493.4	NP_511048.2	minichromosome maintenance 3, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mcm3	NP_032589.1
Arabidopsis thaliana (thale cress)	MCM3	NP_199440.1
Eremothecium gossypii	AGOS_AFR355C	NP_985902.2
Homo sapiens (human)	MCM3	NP_002379.3
Macaca mulatta (Rhesus monkey)	MCM3	XP_001106966.1
Gallus gallus (chicken)	MCM3	NP_001006421.1
Magnaporthe oryzae (rice blast fungus)	MGG_01759	XP_003714758.1
Rattus norvegicus (Norway rat)	Mcm3	NP_001178734.2
Saccharomyces cerevisiae (baker's yeast)	MCM3	NP_010882.1
Oryza sativa (rice)	Os05g0476200	NP_001055835.1
Schizosaccharomyces pombe (fission yeast)	mcm3	NP_587795.1
Kluyveromyces lactis	KLLA0E22243g	XP_454957.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001135	XP_322026.2
Pan troglodytes (chimpanzee)	MCM3	XP_001151750.2
Canis lupus familiaris (dog)	MCM3	XP_538960.3
Danio rerio (zebrafish)	mcm3	NP_997732.1
Drosophila melanogaster (fruit fly)	Mcm3	NP_511048.2
Xenopus tropicalis (tropical clawed frog)	zmcm3	NP_001039231.1
Neurospora crassa	NCU08009	XP_962679.1
Bos taurus (cattle)	MCM3	NP_001013604.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mcm3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mcm3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00845	NM_078493.4 Latest version!	Drosophila melanogaster minichromosome maintenance 3, transcript variant A (Mcm3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa21364	NM_001297955.1 Latest version!	Drosophila melanogaster minichromosome maintenance 3, transcript variant B (Mcm3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mcm3 ( NM_078493.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511048.2 Drosophila melanogaster minichromosome maintenance 3, transcript variant A (Mcm3), mRNA.

Price & Availability↑

CloneID	OFa00845
Clone ID Related Accession (Same CDS sequence)	NM_078493.4
Accession Version	NM_078493.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	minichromosome maintenance 3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078493.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCACG AGGGGGAGCA GTTCATCAAG GACATACAAC GCGAGTATGT GGACTTTCTG 
GACGACGAGG AGGATCAGGG CATCTATGCG GGCCACGTCA AGGATATGAT CGCGGAGAAG 
AGCAAGCGCC TGATCGTGAA TGTCAACGAT CTAAAACGCA AGAATCCGCA ACGTGCATTG 
GGGCTACTGA GCAACGCGGC GGATGAGCAG CTGGCCTTTG GCAGGGCGCT CAAGGAGTAT 
GCGTCCACGG TTGATCCGGG CTATGCCAAA ATGCATGAGG ATCTGTTTGT CGGCTTCGAG 
GGCTGCTTTG GCAATCGGCA TGTGACGCCG CGCTCCCTCA CCTCCATCTA TCTGGGCAAC 
ATGGTGTGCG TGGAGGGCAT TGTTACCAAG GTATCGCTGA TTCGTCCCAA GGTGGTGCGC 
AGCGTTCACT ATTGCCCCAA TACGCGTAAG GTAATGGAGC GCAAATACAC CGATCTCACA 
TCGTTCGAGG CTGTGCCATC TGGCGCTGCC TATCCCACAA AGGACGAGGA TGGCAATCTG 
CTGGAGACCG AATACGGTTT GTCCGTTTAC AAGGATCACC AGACGCTGAC CATTCAAGAG 
ATGCCCGAGA AGGCGCCGGC TGGCCAATTG CCGCGTTCGG TGGATATTGT GTGCGACGAC 
GATCTCGTGG ATCGCTGCAA GCCCGGCGAT CGTGTACAGA TTGTGGGCAG CTATCGCTGT 
TTGCCGGGCA AACGCGGTGG CTACACCTCG GGCACGTTCC GCACCGTGCT GCTGGCCAAC 
AATATATCGC TGCTCAGCAA GGAGAGCAAT TTGGACATAT CGCGCGAAGA CATCATGCTG 
TGCAAGAAGC TAGCGAAGAA TAACGACATC TTCGAGCTGC TCAGCAAGAG TTTGGCGCCC 
TCGATTCATG GACATGCCTA TGTGAAGCAG GCCATTCTTT GCCTGCTGCT CGGCGGCGTT 
GAGAAGATCC TGCCGAATGG CACACGCCTG CGTGGCGATA TTAATGTCTT GCTTATCGGC 
GATCCTTCGG TGGCCAAGTC GCAGCTACTG CGCTACGTCT TGAATACGGC ACCAAGGGCC 
ATACCGACAA CGGGTCGCGG ATCCAGCGGC GTCGGTCTGA CGGCGGCGGT TACCACGGAT 
CAGGAGACCG GTGAGCGGCG ATTGGAGGCG GGCGCCATGG TCCTGGCCGA TCGCGGTGTC 
GTCTGCATTG ATGAGTTTGA CAAGATGAGC GACATCGATC GCACAGCCAT TCACGAGGTG 
ATGGAACAGG GTCGTGTTAC CATTTCCAAA GCGGGCATCC ATGCCTCGCT CAATGCTCGC 
TGCTCCGTTT TGGCAGCCGC TAATCCGGTG TACGGAAGGT ATGATCAATA TAAGACGCCG 
ATGGAAAATA TTGGACTGCA GGATTCGCTG CTTTCGCGTT TCGATTTGCT CTTCGTTATG 
CTGGACGTGA TTGACAGCGA TGTGGATCAA ATGATTTCGG ACCATGTGGT GCGCATGCAT 
CGCTATCGCA ATCCCAAGGA GGCCGACGGC GAGCCCTTGT CCATGGGCAG CTCCTATGCG 
GACTCCTTGT CCTTTGTTTC CAGCAGCGAG GAGAAGAAGG ACACCGAGGT GTACGAAAAG 
TACGATGCCC TGCTGCATGG CAAGTCGCGT CAGCGTCACG AGAAGATCCT ATCCGTGGAG 
TTCATGCGCA AGTACATACA CATTGCCAAG TGTATGAAGC CCAAATTGGG CGAGCAGGCC 
TGCGAGGCGA TTGCCAATGA GTATTCGCGC CTGCGCTCCC AGGAGGCGGT CGAAACGGAT 
GTGGCACGCA CGCAGCCAAT AACGGCCCGT ACCCTGGAGA CCCTCATCCG TTTGTCCACG 
GCCCATGCCC GGGCACGCAT GTCCAAATCG GTGACCATCG ACGATGCCCA TGCGGCCATC 
GAACTGGTAC AGTTTGCCTA CTTTAAGAAG GTGCTGGATA AGGATCGCCC CAGCAAGCGT 
CGTCGCAACA GCGGCTCCGA TGCGGAGGAT GACAACGGTG AGGCATCTAG CCAGCGCTCA 
CCATCGCGAC GCTCCAAACG CACACGCACA GCGACCGTCG GTGCCGATAG CGATGAGGAG 
GATATTGAGC CACCACAGCC GGATGCTGGC GATTTGACGC GTCGCGAAAC GCGTCGTTCT 
CTGCCCGCTC GCTCGGTCGC CATGCTGATG GCCAGCCCCT CCAGTGAGGA GCAGTCGGTG 
GCCACATCGA CCACGGAGCC GGCCATCATT AGCGATGCCC GCCTGGGCGA GTTCAAGAAC 
AATTTGCAGC GACTGTTTCG CGAGGCACGC GAACAGAGCT TGGCGCTGGC CAGGATCACC 
ACGGCCATCA ACGTTGGCAG CCAGGAGCCG TTCACCGCTG GCGAAATCGA GGCCGCCGTG 
CATCGCATGA CCGAGGACAA TCAGATCATG GTCGCCGACG ATATAGTCTT TCTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511048.2
CDS	143..2602
Translation	MAHEGEQFIKDIQREYVDFLDDEEDQGIYAGHVKDMIAEKSKRLIVNVNDLKRKNPQRALGLLSNAADEQLAFGRALKEYASTVDPGYAKMHEDLFVGFEGCFGNRHVTPRSLTSIYLGNMVCVEGIVTKVSLIRPKVVRSVHYCPNTRKVMERKYTDLTSFEAVPSGAAYPTKDEDGNLLETEYGLSVYKDHQTLTIQEMPEKAPAGQLPRSVDIVCDDDLVDRCKPGDRVQIVGSYRCLPGKRGGYTSGTFRTVLLANNISLLSKESNLDISREDIMLCKKLAKNNDIFELLSKSLAPSIHGHAYVKQAILCLLLGGVEKILPNGTRLRGDINVLLIGDPSVAKSQLLRYVLNTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDIDRTAIHEVMEQGRVTISKAGIHASLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFVMLDVIDSDVDQMISDHVVRMHRYRNPKEADGEPLSMGSSYADSLSFVSSSEEKKDTEVYEKYDALLHGKSRQRHEKILSVEFMRKYIHIAKCMKPKLGEQACEAIANEYSRLRSQEAVETDVARTQPITARTLETLIRLSTAHARARMSKSVTIDDAHAAIELVQFAYFKKVLDKDRPSKRRRNSGSDAEDDNGEASSQRSPSRRSKRTRTATVGADSDEEDIEPPQPDAGDLTRRETRRSLPARSVAMLMASPSSEEQSVATSTTEPAIISDARLGEFKNNLQRLFREAREQSLALARITTAINVGSQEPFTAGEIEAAVHRMTEDNQIMVADDIVFLI

Target ORF information:

RefSeq Version	NM_078493.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster minichromosome maintenance 3, transcript variant A (Mcm3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078493.4

ORF Insert Sequence:

ATGGCCCACG AGGGGGAGCA GTTCATCAAG GACATACAAC GCGAGTATGT GGACTTTCTG 
GACGACGAGG AGGATCAGGG CATCTATGCG GGCCACGTCA AGGATATGAT CGCGGAGAAG 
AGCAAGCGCC TGATCGTGAA TGTCAACGAT CTAAAACGCA AGAATCCGCA ACGTGCATTG 
GGGCTACTGA GCAACGCGGC GGATGAGCAG CTGGCCTTTG GCAGGGCGCT CAAGGAGTAT 
GCGTCCACGG TTGATCCGGG CTATGCCAAA ATGCATGAGG ATCTGTTTGT CGGCTTCGAG 
GGCTGCTTTG GCAATCGGCA TGTGACGCCG CGCTCCCTCA CCTCCATCTA TCTGGGCAAC 
ATGGTGTGCG TGGAGGGCAT TGTTACCAAG GTATCGCTGA TTCGTCCCAA GGTGGTGCGC 
AGCGTTCACT ATTGCCCCAA TACGCGTAAG GTAATGGAGC GCAAATACAC CGATCTCACA 
TCGTTCGAGG CTGTGCCATC TGGCGCTGCC TATCCCACAA AGGACGAGGA TGGCAATCTG 
CTGGAGACCG AATACGGTTT GTCCGTTTAC AAGGATCACC AGACGCTGAC CATTCAAGAG 
ATGCCCGAGA AGGCGCCGGC TGGCCAATTG CCGCGTTCGG TGGATATTGT GTGCGACGAC 
GATCTCGTGG ATCGCTGCAA GCCCGGCGAT CGTGTACAGA TTGTGGGCAG CTATCGCTGT 
TTGCCGGGCA AACGCGGTGG CTACACCTCG GGCACGTTCC GCACCGTGCT GCTGGCCAAC 
AATATATCGC TGCTCAGCAA GGAGAGCAAT TTGGACATAT CGCGCGAAGA CATCATGCTG 
TGCAAGAAGC TAGCGAAGAA TAACGACATC TTCGAGCTGC TCAGCAAGAG TTTGGCGCCC 
TCGATTCATG GACATGCCTA TGTGAAGCAG GCCATTCTTT GCCTGCTGCT CGGCGGCGTT 
GAGAAGATCC TGCCGAATGG CACACGCCTG CGTGGCGATA TTAATGTCTT GCTTATCGGC 
GATCCTTCGG TGGCCAAGTC GCAGCTACTG CGCTACGTCT TGAATACGGC ACCAAGGGCC 
ATACCGACAA CGGGTCGCGG ATCCAGCGGC GTCGGTCTGA CGGCGGCGGT TACCACGGAT 
CAGGAGACCG GTGAGCGGCG ATTGGAGGCG GGCGCCATGG TCCTGGCCGA TCGCGGTGTC 
GTCTGCATTG ATGAGTTTGA CAAGATGAGC GACATCGATC GCACAGCCAT TCACGAGGTG 
ATGGAACAGG GTCGTGTTAC CATTTCCAAA GCGGGCATCC ATGCCTCGCT CAATGCTCGC 
TGCTCCGTTT TGGCAGCCGC TAATCCGGTG TACGGAAGGT ATGATCAATA TAAGACGCCG 
ATGGAAAATA TTGGACTGCA GGATTCGCTG CTTTCGCGTT TCGATTTGCT CTTCGTTATG 
CTGGACGTGA TTGACAGCGA TGTGGATCAA ATGATTTCGG ACCATGTGGT GCGCATGCAT 
CGCTATCGCA ATCCCAAGGA GGCCGACGGC GAGCCCTTGT CCATGGGCAG CTCCTATGCG 
GACTCCTTGT CCTTTGTTTC CAGCAGCGAG GAGAAGAAGG ACACCGAGGT GTACGAAAAG 
TACGATGCCC TGCTGCATGG CAAGTCGCGT CAGCGTCACG AGAAGATCCT ATCCGTGGAG 
TTCATGCGCA AGTACATACA CATTGCCAAG TGTATGAAGC CCAAATTGGG CGAGCAGGCC 
TGCGAGGCGA TTGCCAATGA GTATTCGCGC CTGCGCTCCC AGGAGGCGGT CGAAACGGAT 
GTGGCACGCA CGCAGCCAAT AACGGCCCGT ACCCTGGAGA CCCTCATCCG TTTGTCCACG 
GCCCATGCCC GGGCACGCAT GTCCAAATCG GTGACCATCG ACGATGCCCA TGCGGCCATC 
GAACTGGTAC AGTTTGCCTA CTTTAAGAAG GTGCTGGATA AGGATCGCCC CAGCAAGCGT 
CGTCGCAACA GCGGCTCCGA TGCGGAGGAT GACAACGGTG AGGCATCTAG CCAGCGCTCA 
CCATCGCGAC GCTCCAAACG CACACGCACA GCGACCGTCG GTGCCGATAG CGATGAGGAG 
GATATTGAGC CACCACAGCC GGATGCTGGC GATTTGACGC GTCGCGAAAC GCGTCGTTCT 
CTGCCCGCTC GCTCGGTCGC CATGCTGATG GCCAGCCCCT CCAGTGAGGA GCAGTCGGTG 
GCCACATCGA CCACGGAGCC GGCCATCATT AGCGATGCCC GCCTGGGCGA GTTCAAGAAC 
AATTTGCAGC GACTGTTTCG CGAGGCACGC GAACAGAGCT TGGCGCTGGC CAGGATCACC 
ACGGCCATCA ACGTTGGCAG CCAGGAGCCG TTCACCGCTG GCGAAATCGA GGCCGCCGTG 
CATCGCATGA CCGAGGACAA TCAGATCATG GTCGCCGACG ATATAGTCTT TCTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcm3 ( NM_001297955.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284884.1 Drosophila melanogaster minichromosome maintenance 3, transcript variant B (Mcm3), mRNA.

Price & Availability↑

CloneID	OFa21364
Clone ID Related Accession (Same CDS sequence)	NM_001297955.1
Accession Version	NM_001297955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	minichromosome maintenance 3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCACG AGGGGGAGCA GTTCATCAAG GACATACAAC GCGAGTATGT GGACTTTCTG 
GACGACGAGG AGGATCAGGG CATCTATGCG GGCCACGTCA AGGATATGAT CGCGGAGAAG 
AGCAAGCGCC TGATCGTGAA TGTCAACGAT CTAAAACGCA AGAATCCGCA ACGTGCATTG 
GGGCTACTGA GCAACGCGGC GGATGAGCAG CTGGCCTTTG GCAGGGCGCT CAAGGAGTAT 
GCGTCCACGG TTGATCCGGG CTATGCCAAA ATGCATGAGG ATCTGTTTGT CGGCTTCGAG 
GGCTGCTTTG GCAATCGGCA TGTGACGCCG CGCTCCCTCA CCTCCATCTA TCTGGGCAAC 
ATGGTGTGCG TGGAGGGCAT TGTTACCAAG GTATCGCTGA TTCGTCCCAA GGTGGTGCGC 
AGCGTTCACT ATTGCCCCAA TACGCGTAAG GTAATGGAGC GCAAATACAC CGATCTCACA 
TCGTTCGAGG CTGTGCCATC TGGCGCTGCC TATCCCACAA AGGACGAGGA TGGCAATCTG 
CTGGAGACCG AATACGGTTT GTCCGTTTAC AAGGATCACC AGACGCTGAC CATTCAAGAG 
ATGCCCGAGA AGGCGCCGGC TGGCCAATTG CCGCGTTCGG TGGATATTGT GTGCGACGAC 
GATCTCGTGG ATCGCTGCAA GCCCGGCGAT CGTGTACAGA TTGTGGGCAG CTATCGCTGT 
TTGCCGGGCA AACGCGGTGG CTACACCTCG GGCACGTTCC GCACCGTGCT GCTGGCCAAC 
AATATATCGC TGCTCAGCAA GGAGAGCAAT TTGGACATAT CGCGCGAAGA CATCATGCTG 
TGCAAGAAGC TAGCGAAGAA TAACGACATC TTCGAGCTGC TCAGCAAGAG TTTGGCGCCC 
TCGATTCATG GACATGCCTA TGTGAAGCAG GCCATTCTTT GCCTGCTGCT CGGCGGCGTT 
GAGAAGATCC TGCCGAATGG CACACGCCTG CGTGGCGATA TTAATGTCTT GCTTATCGGC 
GATCCTTCGG TGGCCAAGTC GCAGCTACTG CGCTACGTCT TGAATACGGC ACCAAGGGCC 
ATACCGACAA CGGGTCGCGG ATCCAGCGGC GTCGGTCTGA CGGCGGCGGT TACCACGGAT 
CAGGAGACCG GTGAGCGGCG ATTGGAGGCG GGCGCCATGG TCCTGGCCGA TCGCGGTGTC 
GTCTGCATTG ATGAGTTTGA CAAGATGAGC GACATCGATC GCACAGCCAT TCACGAGGTG 
ATGGAACAGG GTCGTGTTAC CATTTCCAAA GCGGGCATCC ATGCCTCGCT CAATGCTCGC 
TGCTCCGTTT TGGCAGCCGC TAATCCGGTG TACGGAAGGT ATGATCAATA TAAGACGCCG 
ATGGAAAATA TTGGACTGCA GGATTCGCTG CTTTCGCGTT TCGATTTGCT CTTCGTTATG 
CTGGACGTGA TTGACAGCGA TGTGGATCAA ATGATTTCGG ACCATGTGGT GCGCATGCAT 
CGCTATCGCA ATCCCAAGGA GGCCGACGGC GAGCCCTTGT CCATGGGCAG CTCCTATGCG 
GACTCCTTGT CCTTTGTTTC CAGCAGCGAG GAGAAGGACA CCGAGGTGTA CGAAAAGTAC 
GATGCCCTGC TGCATGGCAA GTCGCGTCAG CGTCACGAGA AGATCCTATC CGTGGAGTTC 
ATGCGCAAGT ACATACACAT TGCCAAGTGT ATGAAGCCCA AATTGGGCGA GCAGGCCTGC 
GAGGCGATTG CCAATGAGTA TTCGCGCCTG CGCTCCCAGG AGGCGGTCGA AACGGATGTG 
GCACGCACGC AGCCAATAAC GGCCCGTACC CTGGAGACCC TCATCCGTTT GTCCACGGCC 
CATGCCCGGG CACGCATGTC CAAATCGGTG ACCATCGACG ATGCCCATGC GGCCATCGAA 
CTGGTACAGT TTGCCTACTT TAAGAAGGTG CTGGATAAGG ATCGCCCCAG CAAGCGTCGT 
CGCAACAGCG GCTCCGATGC GGAGGATGAC AACGGTGAGG CATCTAGCCA GCGCTCACCA 
TCGCGACGCT CCAAACGCAC ACGCACAGCG ACCGTCGGTG CCGATAGCGA TGAGGAGGAT 
ATTGAGCCAC CACAGCCGGA TGCTGGCGAT TTGACGCGTC GCGAAACGCG TCGTTCTCTG 
CCCGCTCGCT CGGTCGCCAT GCTGATGGCC AGCCCCTCCA GTGAGGAGCA GTCGGTGGCC 
ACATCGACCA CGGAGCCGGC CATCATTAGC GATGCCCGCC TGGGCGAGTT CAAGAACAAT 
TTGCAGCGAC TGTTTCGCGA GGCACGCGAA CAGAGCTTGG CGCTGGCCAG GATCACCACG 
GCCATCAACG TTGGCAGCCA GGAGCCGTTC ACCGCTGGCG AAATCGAGGC CGCCGTGCAT 
CGCATGACCG AGGACAATCA GATCATGGTC GCCGACGATA TAGTCTTTCT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284884.1
CDS	143..2599
Translation	MAHEGEQFIKDIQREYVDFLDDEEDQGIYAGHVKDMIAEKSKRLIVNVNDLKRKNPQRALGLLSNAADEQLAFGRALKEYASTVDPGYAKMHEDLFVGFEGCFGNRHVTPRSLTSIYLGNMVCVEGIVTKVSLIRPKVVRSVHYCPNTRKVMERKYTDLTSFEAVPSGAAYPTKDEDGNLLETEYGLSVYKDHQTLTIQEMPEKAPAGQLPRSVDIVCDDDLVDRCKPGDRVQIVGSYRCLPGKRGGYTSGTFRTVLLANNISLLSKESNLDISREDIMLCKKLAKNNDIFELLSKSLAPSIHGHAYVKQAILCLLLGGVEKILPNGTRLRGDINVLLIGDPSVAKSQLLRYVLNTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDIDRTAIHEVMEQGRVTISKAGIHASLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFVMLDVIDSDVDQMISDHVVRMHRYRNPKEADGEPLSMGSSYADSLSFVSSSEEKDTEVYEKYDALLHGKSRQRHEKILSVEFMRKYIHIAKCMKPKLGEQACEAIANEYSRLRSQEAVETDVARTQPITARTLETLIRLSTAHARARMSKSVTIDDAHAAIELVQFAYFKKVLDKDRPSKRRRNSGSDAEDDNGEASSQRSPSRRSKRTRTATVGADSDEEDIEPPQPDAGDLTRRETRRSLPARSVAMLMASPSSEEQSVATSTTEPAIISDARLGEFKNNLQRLFREAREQSLALARITTAINVGSQEPFTAGEIEAAVHRMTEDNQIMVADDIVFLI

Target ORF information:

RefSeq Version	NM_001297955.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster minichromosome maintenance 3, transcript variant B (Mcm3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297955.1

ORF Insert Sequence:

ATGGCCCACG AGGGGGAGCA GTTCATCAAG GACATACAAC GCGAGTATGT GGACTTTCTG 
GACGACGAGG AGGATCAGGG CATCTATGCG GGCCACGTCA AGGATATGAT CGCGGAGAAG 
AGCAAGCGCC TGATCGTGAA TGTCAACGAT CTAAAACGCA AGAATCCGCA ACGTGCATTG 
GGGCTACTGA GCAACGCGGC GGATGAGCAG CTGGCCTTTG GCAGGGCGCT CAAGGAGTAT 
GCGTCCACGG TTGATCCGGG CTATGCCAAA ATGCATGAGG ATCTGTTTGT CGGCTTCGAG 
GGCTGCTTTG GCAATCGGCA TGTGACGCCG CGCTCCCTCA CCTCCATCTA TCTGGGCAAC 
ATGGTGTGCG TGGAGGGCAT TGTTACCAAG GTATCGCTGA TTCGTCCCAA GGTGGTGCGC 
AGCGTTCACT ATTGCCCCAA TACGCGTAAG GTAATGGAGC GCAAATACAC CGATCTCACA 
TCGTTCGAGG CTGTGCCATC TGGCGCTGCC TATCCCACAA AGGACGAGGA TGGCAATCTG 
CTGGAGACCG AATACGGTTT GTCCGTTTAC AAGGATCACC AGACGCTGAC CATTCAAGAG 
ATGCCCGAGA AGGCGCCGGC TGGCCAATTG CCGCGTTCGG TGGATATTGT GTGCGACGAC 
GATCTCGTGG ATCGCTGCAA GCCCGGCGAT CGTGTACAGA TTGTGGGCAG CTATCGCTGT 
TTGCCGGGCA AACGCGGTGG CTACACCTCG GGCACGTTCC GCACCGTGCT GCTGGCCAAC 
AATATATCGC TGCTCAGCAA GGAGAGCAAT TTGGACATAT CGCGCGAAGA CATCATGCTG 
TGCAAGAAGC TAGCGAAGAA TAACGACATC TTCGAGCTGC TCAGCAAGAG TTTGGCGCCC 
TCGATTCATG GACATGCCTA TGTGAAGCAG GCCATTCTTT GCCTGCTGCT CGGCGGCGTT 
GAGAAGATCC TGCCGAATGG CACACGCCTG CGTGGCGATA TTAATGTCTT GCTTATCGGC 
GATCCTTCGG TGGCCAAGTC GCAGCTACTG CGCTACGTCT TGAATACGGC ACCAAGGGCC 
ATACCGACAA CGGGTCGCGG ATCCAGCGGC GTCGGTCTGA CGGCGGCGGT TACCACGGAT 
CAGGAGACCG GTGAGCGGCG ATTGGAGGCG GGCGCCATGG TCCTGGCCGA TCGCGGTGTC 
GTCTGCATTG ATGAGTTTGA CAAGATGAGC GACATCGATC GCACAGCCAT TCACGAGGTG 
ATGGAACAGG GTCGTGTTAC CATTTCCAAA GCGGGCATCC ATGCCTCGCT CAATGCTCGC 
TGCTCCGTTT TGGCAGCCGC TAATCCGGTG TACGGAAGGT ATGATCAATA TAAGACGCCG 
ATGGAAAATA TTGGACTGCA GGATTCGCTG CTTTCGCGTT TCGATTTGCT CTTCGTTATG 
CTGGACGTGA TTGACAGCGA TGTGGATCAA ATGATTTCGG ACCATGTGGT GCGCATGCAT 
CGCTATCGCA ATCCCAAGGA GGCCGACGGC GAGCCCTTGT CCATGGGCAG CTCCTATGCG 
GACTCCTTGT CCTTTGTTTC CAGCAGCGAG GAGAAGGACA CCGAGGTGTA CGAAAAGTAC 
GATGCCCTGC TGCATGGCAA GTCGCGTCAG CGTCACGAGA AGATCCTATC CGTGGAGTTC 
ATGCGCAAGT ACATACACAT TGCCAAGTGT ATGAAGCCCA AATTGGGCGA GCAGGCCTGC 
GAGGCGATTG CCAATGAGTA TTCGCGCCTG CGCTCCCAGG AGGCGGTCGA AACGGATGTG 
GCACGCACGC AGCCAATAAC GGCCCGTACC CTGGAGACCC TCATCCGTTT GTCCACGGCC 
CATGCCCGGG CACGCATGTC CAAATCGGTG ACCATCGACG ATGCCCATGC GGCCATCGAA 
CTGGTACAGT TTGCCTACTT TAAGAAGGTG CTGGATAAGG ATCGCCCCAG CAAGCGTCGT 
CGCAACAGCG GCTCCGATGC GGAGGATGAC AACGGTGAGG CATCTAGCCA GCGCTCACCA 
TCGCGACGCT CCAAACGCAC ACGCACAGCG ACCGTCGGTG CCGATAGCGA TGAGGAGGAT 
ATTGAGCCAC CACAGCCGGA TGCTGGCGAT TTGACGCGTC GCGAAACGCG TCGTTCTCTG 
CCCGCTCGCT CGGTCGCCAT GCTGATGGCC AGCCCCTCCA GTGAGGAGCA GTCGGTGGCC 
ACATCGACCA CGGAGCCGGC CATCATTAGC GATGCCCGCC TGGGCGAGTT CAAGAACAAT 
TTGCAGCGAC TGTTTCGCGA GGCACGCGAA CAGAGCTTGG CGCTGGCCAG GATCACCACG 
GCCATCAACG TTGGCAGCCA GGAGCCGTTC ACCGCTGGCG AAATCGAGGC CGCCGTGCAT 
CGCATGACCG AGGACAATCA GATCATGGTC GCCGACGATA TAGTCTTTCT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mcm3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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