Nmd3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nmd3
Entrez Gene ID	31195
Full Name	Nonsense-mediated mRNA 3
Synonyms	CG3460,Dmel\CG3460,EG:152A3.1,II,TcD12,TcD13,anon-2Eb,dNmd3
General protein information	Preferred Names Nonsense-mediated mRNA 3 Names CG3460 gene product from transcript CG3460-RA CG3460-PA Nmd3-PA transcription unit III
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2E1-2E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057984.5	NP_477332.1	Nonsense-mediated mRNA 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NMD3	XP_545261.3
Caenorhabditis elegans (roundworm)	T25G3.3	NP_492114.2
Oryza sativa (rice)	Os10g0573900	NP_001065473.1
Kluyveromyces lactis	KLLA0A05159g	XP_451227.1
Rattus norvegicus (Norway rat)	Nmd3	NP_001101152.1
Danio rerio (zebrafish)	nmd3	NP_001013587.1
Saccharomyces cerevisiae (baker's yeast)	NMD3	NP_012040.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005375	XP_315383.4
Schizosaccharomyces pombe (fission yeast)	nmd3	NP_593077.1
Macaca mulatta (Rhesus monkey)	NMD3	XP_001096663.2
Mus musculus (house mouse)	Nmd3	NP_598548.1
Neurospora crassa	NCU08663	XP_961964.1
Arabidopsis thaliana (thale cress)	AT2G03820	NP_178476.1
Xenopus tropicalis (tropical clawed frog)	nmd3	NP_989367.2
Magnaporthe oryzae (rice blast fungus)	MGG_05817	XP_003712132.1
Homo sapiens (human)	NMD3	NP_057022.2
Pan troglodytes (chimpanzee)	NMD3	XP_516854.2
Drosophila melanogaster (fruit fly)	Nmd3	NP_477332.1
Bos taurus (cattle)	NMD3	NP_001068888.1
Gallus gallus (chicken)	NMD3	XP_422813.2
Eremothecium gossypii	AGOS_AER299C	NP_985156.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nmd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nmd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00572	NM_057984.5 Latest version!	Drosophila melanogaster Nonsense-mediated mRNA 3 (Nmd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nmd3 ( NM_057984.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477332.1 Drosophila melanogaster Nonsense-mediated mRNA 3 (Nmd3), mRNA.

Price & Availability↑

CloneID	OFa00572
Clone ID Related Accession (Same CDS sequence)	NM_057984.5
Accession Version	NM_057984.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Nonsense-mediated mRNA 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057984.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCACG AATACGTTGG GGTTAGCCCG CTGGGCGCAG CGGACGGCAA GGGCGCCGGA 
TCGAACGCCG TCATCCTGTG CTGCGAGTGC GGAGTGGCCA TCCAGCCGAA TCCGGCCAAC 
ATGTGCGTCA CCTGCCTGCG CAACCATGTC GACATCACGG AGAACATCCC AAAGCAGGCC 
GTGCTCCACT TCTGTCGTAA TTGCGAGCGC TATCTGCAAC CGCCGAACGA GTGGATACAG 
GCCGCCTTGG AGTCCCGCGA ACTCTTGGCC ATTTGCCTTA AGAAGCTAAA AGGCCTGAAG 
GAAGTGAAGT TGGTGGATGC CGGCTTCATC TGGACAGAAC AGCACTCCAA GCGCATAAAG 
GTGAAGCTGA CTGTGCATGG GGAGATCATC GGGGGAACGA TGCTCCAGCA GGTATTCGTG 
GTGGAGTTCA CTGTGCAGAA CCAGATGTGC AACGACTGCC ATCGGACCGA GGCCAAGGAC 
TTCTGGCGCT GCCTCGTGCA GGTTCGTCAG CGGGCCGAGA ACAAGAAAAC CTTTTACTAT 
CTGGAACAGC TGATCCTTAA GCACAAAGCC CACGAGAACA CTCTGGGCAT AAAGCCGGAG 
CATGGCGGCC TGGATTTCTT CTATGCCAAT GATAATCATG CCCGGCGCAT GGTTGATTTC 
CTCCTGACCA TGGTGCCCGG CAAGGTAACA ACCTCGAAGC GTCTCATCTC TCACGACATC 
CACAGCAACA ACTATAACTA CAAGTACAAC TGGTCAGTGG AAATAGCACC AGTATCGAAG 
GACAGTGCAG TTTGCCTGTC CAAAAAGCTG CGCCACCAGC TGGGAAATCT CTCGCCATTG 
GTTCTGGTCA ACCGGGTGTC TAGCAGCATT CACTTGATTG ATCCCCTGAC CGCACAGATT 
GCAGAACTCA CCTCGCAGGT CTATTTCAGA GCTCCCTTTG AGGCCGTCTG CAATCCCAAG 
CAGCTGGTTG AGTACGTCGT CATGGACATT GATGTGATCA TGGAAAAGGA TCGAAAGACC 
TATCCTGGTC AAGGGCAGGT TTCCTTCAAG CACGCTCTCT GCGACATTTG GGTGGTGCGC 
GCCTCGGAGC TGGGCATCAA CGATAACCCC ATTCACACAC GCTCCCACTT GGGCCACCTG 
CTCAAGGTGG GCGATTCCGT AATGGGCTAC AACACCGGAG AGGCCAACAT CAACGATCTG 
GAGTTCGACA AATTGTCGTC GGATGAAATT CCGGATGTAA TTCTTGTGCG CAAGCACTAC 
GATCGACAGA CCAGACTCAG TCAGCGCGTG TGGAAGATTA AGCATCTGGC GGCGGAGGCT 
ACCGATGAGC GCAGCAAGTA CGACTACCAC GAGTTTCTTG ACGATCTCGA GGAAAACGAG 
GATATCCGCG GTCAGGTCAA CATCTATCGG GACACTAACA AGACGATACC AATCGAGGTA 
CAGGCCGCTG GAGGTGATGT GCCCCAGATC ACACTGGAGG AGATGTTGGA AGATATGACA 
TTAGAGTGTG CAGACGACGA AATGGGCGAG GAGGGAGGTG CTGACGATAC CGAGCCACAG 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477332.1
CDS	131..1696
Translation	MDHEYVGVSPLGAADGKGAGSNAVILCCECGVAIQPNPANMCVTCLRNHVDITENIPKQAVLHFCRNCERYLQPPNEWIQAALESRELLAICLKKLKGLKEVKLVDAGFIWTEQHSKRIKVKLTVHGEIIGGTMLQQVFVVEFTVQNQMCNDCHRTEAKDFWRCLVQVRQRAENKKTFYYLEQLILKHKAHENTLGIKPEHGGLDFFYANDNHARRMVDFLLTMVPGKVTTSKRLISHDIHSNNYNYKYNWSVEIAPVSKDSAVCLSKKLRHQLGNLSPLVLVNRVSSSIHLIDPLTAQIAELTSQVYFRAPFEAVCNPKQLVEYVVMDIDVIMEKDRKTYPGQGQVSFKHALCDIWVVRASELGINDNPIHTRSHLGHLLKVGDSVMGYNTGEANINDLEFDKLSSDEIPDVILVRKHYDRQTRLSQRVWKIKHLAAEATDERSKYDYHEFLDDLEENEDIRGQVNIYRDTNKTIPIEVQAAGGDVPQITLEEMLEDMTLECADDEMGEEGGADDTEPQL

Target ORF information:

RefSeq Version	NM_057984.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nonsense-mediated mRNA 3 (Nmd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057984.5

ORF Insert Sequence:

ATGGATCACG AATACGTTGG GGTTAGCCCG CTGGGCGCAG CGGACGGCAA GGGCGCCGGA 
TCGAACGCCG TCATCCTGTG CTGCGAGTGC GGAGTGGCCA TCCAGCCGAA TCCGGCCAAC 
ATGTGCGTCA CCTGCCTGCG CAACCATGTC GACATCACGG AGAACATCCC AAAGCAGGCC 
GTGCTCCACT TCTGTCGTAA TTGCGAGCGC TATCTGCAAC CGCCGAACGA GTGGATACAG 
GCCGCCTTGG AGTCCCGCGA ACTCTTGGCC ATTTGCCTTA AGAAGCTAAA AGGCCTGAAG 
GAAGTGAAGT TGGTGGATGC CGGCTTCATC TGGACAGAAC AGCACTCCAA GCGCATAAAG 
GTGAAGCTGA CTGTGCATGG GGAGATCATC GGGGGAACGA TGCTCCAGCA GGTATTCGTG 
GTGGAGTTCA CTGTGCAGAA CCAGATGTGC AACGACTGCC ATCGGACCGA GGCCAAGGAC 
TTCTGGCGCT GCCTCGTGCA GGTTCGTCAG CGGGCCGAGA ACAAGAAAAC CTTTTACTAT 
CTGGAACAGC TGATCCTTAA GCACAAAGCC CACGAGAACA CTCTGGGCAT AAAGCCGGAG 
CATGGCGGCC TGGATTTCTT CTATGCCAAT GATAATCATG CCCGGCGCAT GGTTGATTTC 
CTCCTGACCA TGGTGCCCGG CAAGGTAACA ACCTCGAAGC GTCTCATCTC TCACGACATC 
CACAGCAACA ACTATAACTA CAAGTACAAC TGGTCAGTGG AAATAGCACC AGTATCGAAG 
GACAGTGCAG TTTGCCTGTC CAAAAAGCTG CGCCACCAGC TGGGAAATCT CTCGCCATTG 
GTTCTGGTCA ACCGGGTGTC TAGCAGCATT CACTTGATTG ATCCCCTGAC CGCACAGATT 
GCAGAACTCA CCTCGCAGGT CTATTTCAGA GCTCCCTTTG AGGCCGTCTG CAATCCCAAG 
CAGCTGGTTG AGTACGTCGT CATGGACATT GATGTGATCA TGGAAAAGGA TCGAAAGACC 
TATCCTGGTC AAGGGCAGGT TTCCTTCAAG CACGCTCTCT GCGACATTTG GGTGGTGCGC 
GCCTCGGAGC TGGGCATCAA CGATAACCCC ATTCACACAC GCTCCCACTT GGGCCACCTG 
CTCAAGGTGG GCGATTCCGT AATGGGCTAC AACACCGGAG AGGCCAACAT CAACGATCTG 
GAGTTCGACA AATTGTCGTC GGATGAAATT CCGGATGTAA TTCTTGTGCG CAAGCACTAC 
GATCGACAGA CCAGACTCAG TCAGCGCGTG TGGAAGATTA AGCATCTGGC GGCGGAGGCT 
ACCGATGAGC GCAGCAAGTA CGACTACCAC GAGTTTCTTG ACGATCTCGA GGAAAACGAG 
GATATCCGCG GTCAGGTCAA CATCTATCGG GACACTAACA AGACGATACC AATCGAGGTA 
CAGGCCGCTG GAGGTGATGT GCCCCAGATC ACACTGGAGG AGATGTTGGA AGATATGACA 
TTAGAGTGTG CAGACGACGA AATGGGCGAG GAGGGAGGTG CTGACGATAC CGAGCCACAG 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nmd3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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