NT5E-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	NT5E-2
Entrez Gene ID	246458
Full Name	Ecto-5'-nucleotidase 2
Synonyms	CG30104,CG4837,Dmel\CG30104
General protein information	Preferred Names Ecto-5'-nucleotidase 2 Names CG30104 gene product from transcript CG30104-RA CG30104-PA CG30104-PB NT5E-2-PA NT5E-2-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54B17-54B17

mRNA and Protein(s)

mRNA	Protein	Name
NM_137374.3	NP_611218.1	Ecto-5'-nucleotidase 2, isoform A
NM_166232.2	NP_725681.1	Ecto-5'-nucleotidase 2, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NT5E	XP_518619.3
Gallus gallus (chicken)	NT5E	XP_004940454.1
Danio rerio (zebrafish)	nt5e	NP_957226.1
Drosophila melanogaster (fruit fly)	NT5E-2	NP_611218.1
Xenopus tropicalis (tropical clawed frog)	nt5e	XP_002933973.1
Canis lupus familiaris (dog)	NT5E	XP_532221.2
Rattus norvegicus (Norway rat)	Nt5e	NP_067587.2
Drosophila melanogaster (fruit fly)	veil	NP_611217.1
Homo sapiens (human)	NT5E	NP_002517.1
Macaca mulatta (Rhesus monkey)	NT5E	XP_001086989.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003629	XP_313394.4
Magnaporthe oryzae (rice blast fungus)	MGG_12474	XP_003715781.1
Mus musculus (house mouse)	Nt5e	NP_035981.1
Neurospora crassa	NCU09659	XP_958725.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NT5E-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NT5E-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11607	NM_137374.3 Latest version!	Drosophila melanogaster Ecto-5'-nucleotidase 2 (NT5E-2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa11607	NM_166232.2 Latest version!	Drosophila melanogaster Ecto-5'-nucleotidase 2 (NT5E-2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NT5E-2 ( NM_137374.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611218.1 Drosophila melanogaster Ecto-5'-nucleotidase 2 (NT5E-2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11607
Clone ID Related Accession (Same CDS sequence)	NM_137374.3 , NM_166232.2
Accession Version	NM_137374.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Ecto-5'-nucleotidase 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137374.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTCAC GTTGGCTTTG CGGGATCTTC GTGATCGTCC TGGCCCATTG GACACGGGCC 
AATCCAATTT TGAGCCATCC CAAAGTGGCC ACCGAGTTCA TCATCCTCCA TAATAATGAT 
ATGCATGCCC GTTTCGATCA GACGAGCGTT AATAGCGGCA CCTGCCCACC GGAGGATGTA 
CATACGAATA AATGCTATGG AGGATTCGCC AGGGTGGCCC ACGAAGTTCG AAAGTATCGT 
AAGGAGGCTC AGGAGGGCGG TACCTCCGTA TTGTACTTAA ATGCTGGTGA TACCTACACT 
GGAACCTCTT GGTTTACTAT TTTCAAGGAC AAAATCGCCA GTGCTTTCCT TAACAAATTG 
AAACCGGATG CTATATCCTT GGGCAATCAT GAGTTTGATG AGAGAGTAGA GGGTCTAATA 
CCCTTCCTCA ACGAGGTCAC CTTTCCTGTA CTCGCCTGTA ATCTGGACCT AAGCAAGGTT 
CCCCAACTGA AGGCCACTTG GCACTTGGCT AACTCCGCCA TTCTGGAGAC CAATGGCACT 
AAGGTCGGTG TCATAGGTTA TTTGACTCCA GATACCAAAA AACTTACTCT CAACATGGAT 
GTGGAGTTCA ACGAGGAGGT GGAGTCCATT AATGTTGAAG CAAAAAAACT GAAGGCACAG 
GGCATCAAAA TCATTATCGC TCTTGGCCAC TCGGGTTATT TGAAGGATCT GGAGATAGCC 
AAGAACTGTC CGGAGGTGGA TATTGTCATT GGTGGCCATA CGAACACCTT TCTCTACACC 
GGTGCCCAGC CGGATGCGGA GCACATAGAT GGACCGTATC CAACGATGGT CAAGCAGAAC 
AGTGGCAAGG AGGTTCCCGT TGTGCAGGCC TATGCGTATA CCAAATATTT GGGAAAACTG 
CATGTACAGT TTGATGCCGA TGGCAACCTC ATCCAGTGGG ATGGATCTCC CATCCTGCTC 
AATGCTTCAG TGGCTCAGGA GCAGGACCTT CTCGATCTAT TGGAGGTCTT TCGTCCGAAT 
GTCACCAGGC TGGAGAAGTC GGTCGTCGGG CACACCAAGG TTCATCTGGA GGGCAACAAG 
GCGGTTTGCC GGGCCGAGGA GTGCAACCTG GGCAATCTGA TAGCCGATGC AATGGTGTTT 
AGTCGCCTGA TGGAGGAGCA GGGCGGTGAT TTCTGGACAG ATGCTGCCAT TTCTATAATG 
CAGGGCGGAG GCATTCGCAG CTCCATTGAA AAGCGATCGG ATGGCGCCAT CACCGACAAC 
GATATCCTTT CCGTTCTGCC CTGGGGTAAT AAGCTCTATA TGGTGCCCAT GACGGGTAGC 
ACTATTCGTC GAGCCTTAGA GCATGGAGCT GCCCTGAGAG GCAAAGATTC GGACGGTGGC 
TTCCTGCAGG TGTCCGGAAT CCGTGTGGTT TTCAATTCGA ACAAGCCCGA GGGTCAGCGA 
GTGGTTTCCG TGCAGGTTCG ATGTGCCGCC TGCCGAGTGC CCACCTATAG TGATCTGAAT 
GATACTGCCA TCTACAATGT CGTCCTGGGA GAGTTCCTGC TAGATGGTGG CGATGGACAT 
GTAATGAGGG ACTCAGCCCA CCAACCACAG CGCCTGCAAA ATAACGATCT CGAGGCGGTA 
TCCCAATATC TGAACCAGCG TGACTATGTC TATCCCGAGA TCGAGGGTCG TATTATTTTC 
ATTAATGCAT CCAGTACACT TATGGGCTCG GCAGCCCTGC TTCTTATCTC TTCCCTTCTG 
ATAAAAATTA TTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611218.1
CDS	134..1891
Translation	MQSRWLCGIFVIVLAHWTRANPILSHPKVATEFIILHNNDMHARFDQTSVNSGTCPPEDVHTNKCYGGFARVAHEVRKYRKEAQEGGTSVLYLNAGDTYTGTSWFTIFKDKIASAFLNKLKPDAISLGNHEFDERVEGLIPFLNEVTFPVLACNLDLSKVPQLKATWHLANSAILETNGTKVGVIGYLTPDTKKLTLNMDVEFNEEVESINVEAKKLKAQGIKIIIALGHSGYLKDLEIAKNCPEVDIVIGGHTNTFLYTGAQPDAEHIDGPYPTMVKQNSGKEVPVVQAYAYTKYLGKLHVQFDADGNLIQWDGSPILLNASVAQEQDLLDLLEVFRPNVTRLEKSVVGHTKVHLEGNKAVCRAEECNLGNLIADAMVFSRLMEEQGGDFWTDAAISIMQGGGIRSSIEKRSDGAITDNDILSVLPWGNKLYMVPMTGSTIRRALEHGAALRGKDSDGGFLQVSGIRVVFNSNKPEGQRVVSVQVRCAACRVPTYSDLNDTAIYNVVLGEFLLDGGDGHVMRDSAHQPQRLQNNDLEAVSQYLNQRDYVYPEIEGRIIFINASSTLMGSAALLLISSLLIKIIA

Target ORF information:

RefSeq Version	NM_137374.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecto-5'-nucleotidase 2 (NT5E-2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137374.3

ORF Insert Sequence:

ATGCAGTCAC GTTGGCTTTG CGGGATCTTC GTGATCGTCC TGGCCCATTG GACACGGGCC 
AATCCAATTT TGAGCCATCC CAAAGTGGCC ACCGAGTTCA TCATCCTCCA TAATAATGAT 
ATGCATGCCC GTTTCGATCA GACGAGCGTT AATAGCGGCA CCTGCCCACC GGAGGATGTA 
CATACGAATA AATGCTATGG AGGATTCGCC AGGGTGGCCC ACGAAGTTCG AAAGTATCGT 
AAGGAGGCTC AGGAGGGCGG TACCTCCGTA TTGTACTTAA ATGCTGGTGA TACCTACACT 
GGAACCTCTT GGTTTACTAT TTTCAAGGAC AAAATCGCCA GTGCTTTCCT TAACAAATTG 
AAACCGGATG CTATATCCTT GGGCAATCAT GAGTTTGATG AGAGAGTAGA GGGTCTAATA 
CCCTTCCTCA ACGAGGTCAC CTTTCCTGTA CTCGCCTGTA ATCTGGACCT AAGCAAGGTT 
CCCCAACTGA AGGCCACTTG GCACTTGGCT AACTCCGCCA TTCTGGAGAC CAATGGCACT 
AAGGTCGGTG TCATAGGTTA TTTGACTCCA GATACCAAAA AACTTACTCT CAACATGGAT 
GTGGAGTTCA ACGAGGAGGT GGAGTCCATT AATGTTGAAG CAAAAAAACT GAAGGCACAG 
GGCATCAAAA TCATTATCGC TCTTGGCCAC TCGGGTTATT TGAAGGATCT GGAGATAGCC 
AAGAACTGTC CGGAGGTGGA TATTGTCATT GGTGGCCATA CGAACACCTT TCTCTACACC 
GGTGCCCAGC CGGATGCGGA GCACATAGAT GGACCGTATC CAACGATGGT CAAGCAGAAC 
AGTGGCAAGG AGGTTCCCGT TGTGCAGGCC TATGCGTATA CCAAATATTT GGGAAAACTG 
CATGTACAGT TTGATGCCGA TGGCAACCTC ATCCAGTGGG ATGGATCTCC CATCCTGCTC 
AATGCTTCAG TGGCTCAGGA GCAGGACCTT CTCGATCTAT TGGAGGTCTT TCGTCCGAAT 
GTCACCAGGC TGGAGAAGTC GGTCGTCGGG CACACCAAGG TTCATCTGGA GGGCAACAAG 
GCGGTTTGCC GGGCCGAGGA GTGCAACCTG GGCAATCTGA TAGCCGATGC AATGGTGTTT 
AGTCGCCTGA TGGAGGAGCA GGGCGGTGAT TTCTGGACAG ATGCTGCCAT TTCTATAATG 
CAGGGCGGAG GCATTCGCAG CTCCATTGAA AAGCGATCGG ATGGCGCCAT CACCGACAAC 
GATATCCTTT CCGTTCTGCC CTGGGGTAAT AAGCTCTATA TGGTGCCCAT GACGGGTAGC 
ACTATTCGTC GAGCCTTAGA GCATGGAGCT GCCCTGAGAG GCAAAGATTC GGACGGTGGC 
TTCCTGCAGG TGTCCGGAAT CCGTGTGGTT TTCAATTCGA ACAAGCCCGA GGGTCAGCGA 
GTGGTTTCCG TGCAGGTTCG ATGTGCCGCC TGCCGAGTGC CCACCTATAG TGATCTGAAT 
GATACTGCCA TCTACAATGT CGTCCTGGGA GAGTTCCTGC TAGATGGTGG CGATGGACAT 
GTAATGAGGG ACTCAGCCCA CCAACCACAG CGCCTGCAAA ATAACGATCT CGAGGCGGTA 
TCCCAATATC TGAACCAGCG TGACTATGTC TATCCCGAGA TCGAGGGTCG TATTATTTTC 
ATTAATGCAT CCAGTACACT TATGGGCTCG GCAGCCCTGC TTCTTATCTC TTCCCTTCTG 
ATAAAAATTA TTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NT5E-2 ( NM_166232.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725681.1 Drosophila melanogaster Ecto-5'-nucleotidase 2 (NT5E-2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11607
Clone ID Related Accession (Same CDS sequence)	NM_137374.3 , NM_166232.2
Accession Version	NM_166232.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Ecto-5'-nucleotidase 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166232.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTCAC GTTGGCTTTG CGGGATCTTC GTGATCGTCC TGGCCCATTG GACACGGGCC 
AATCCAATTT TGAGCCATCC CAAAGTGGCC ACCGAGTTCA TCATCCTCCA TAATAATGAT 
ATGCATGCCC GTTTCGATCA GACGAGCGTT AATAGCGGCA CCTGCCCACC GGAGGATGTA 
CATACGAATA AATGCTATGG AGGATTCGCC AGGGTGGCCC ACGAAGTTCG AAAGTATCGT 
AAGGAGGCTC AGGAGGGCGG TACCTCCGTA TTGTACTTAA ATGCTGGTGA TACCTACACT 
GGAACCTCTT GGTTTACTAT TTTCAAGGAC AAAATCGCCA GTGCTTTCCT TAACAAATTG 
AAACCGGATG CTATATCCTT GGGCAATCAT GAGTTTGATG AGAGAGTAGA GGGTCTAATA 
CCCTTCCTCA ACGAGGTCAC CTTTCCTGTA CTCGCCTGTA ATCTGGACCT AAGCAAGGTT 
CCCCAACTGA AGGCCACTTG GCACTTGGCT AACTCCGCCA TTCTGGAGAC CAATGGCACT 
AAGGTCGGTG TCATAGGTTA TTTGACTCCA GATACCAAAA AACTTACTCT CAACATGGAT 
GTGGAGTTCA ACGAGGAGGT GGAGTCCATT AATGTTGAAG CAAAAAAACT GAAGGCACAG 
GGCATCAAAA TCATTATCGC TCTTGGCCAC TCGGGTTATT TGAAGGATCT GGAGATAGCC 
AAGAACTGTC CGGAGGTGGA TATTGTCATT GGTGGCCATA CGAACACCTT TCTCTACACC 
GGTGCCCAGC CGGATGCGGA GCACATAGAT GGACCGTATC CAACGATGGT CAAGCAGAAC 
AGTGGCAAGG AGGTTCCCGT TGTGCAGGCC TATGCGTATA CCAAATATTT GGGAAAACTG 
CATGTACAGT TTGATGCCGA TGGCAACCTC ATCCAGTGGG ATGGATCTCC CATCCTGCTC 
AATGCTTCAG TGGCTCAGGA GCAGGACCTT CTCGATCTAT TGGAGGTCTT TCGTCCGAAT 
GTCACCAGGC TGGAGAAGTC GGTCGTCGGG CACACCAAGG TTCATCTGGA GGGCAACAAG 
GCGGTTTGCC GGGCCGAGGA GTGCAACCTG GGCAATCTGA TAGCCGATGC AATGGTGTTT 
AGTCGCCTGA TGGAGGAGCA GGGCGGTGAT TTCTGGACAG ATGCTGCCAT TTCTATAATG 
CAGGGCGGAG GCATTCGCAG CTCCATTGAA AAGCGATCGG ATGGCGCCAT CACCGACAAC 
GATATCCTTT CCGTTCTGCC CTGGGGTAAT AAGCTCTATA TGGTGCCCAT GACGGGTAGC 
ACTATTCGTC GAGCCTTAGA GCATGGAGCT GCCCTGAGAG GCAAAGATTC GGACGGTGGC 
TTCCTGCAGG TGTCCGGAAT CCGTGTGGTT TTCAATTCGA ACAAGCCCGA GGGTCAGCGA 
GTGGTTTCCG TGCAGGTTCG ATGTGCCGCC TGCCGAGTGC CCACCTATAG TGATCTGAAT 
GATACTGCCA TCTACAATGT CGTCCTGGGA GAGTTCCTGC TAGATGGTGG CGATGGACAT 
GTAATGAGGG ACTCAGCCCA CCAACCACAG CGCCTGCAAA ATAACGATCT CGAGGCGGTA 
TCCCAATATC TGAACCAGCG TGACTATGTC TATCCCGAGA TCGAGGGTCG TATTATTTTC 
ATTAATGCAT CCAGTACACT TATGGGCTCG GCAGCCCTGC TTCTTATCTC TTCCCTTCTG 
ATAAAAATTA TTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725681.1
CDS	32..1789
Translation	MQSRWLCGIFVIVLAHWTRANPILSHPKVATEFIILHNNDMHARFDQTSVNSGTCPPEDVHTNKCYGGFARVAHEVRKYRKEAQEGGTSVLYLNAGDTYTGTSWFTIFKDKIASAFLNKLKPDAISLGNHEFDERVEGLIPFLNEVTFPVLACNLDLSKVPQLKATWHLANSAILETNGTKVGVIGYLTPDTKKLTLNMDVEFNEEVESINVEAKKLKAQGIKIIIALGHSGYLKDLEIAKNCPEVDIVIGGHTNTFLYTGAQPDAEHIDGPYPTMVKQNSGKEVPVVQAYAYTKYLGKLHVQFDADGNLIQWDGSPILLNASVAQEQDLLDLLEVFRPNVTRLEKSVVGHTKVHLEGNKAVCRAEECNLGNLIADAMVFSRLMEEQGGDFWTDAAISIMQGGGIRSSIEKRSDGAITDNDILSVLPWGNKLYMVPMTGSTIRRALEHGAALRGKDSDGGFLQVSGIRVVFNSNKPEGQRVVSVQVRCAACRVPTYSDLNDTAIYNVVLGEFLLDGGDGHVMRDSAHQPQRLQNNDLEAVSQYLNQRDYVYPEIEGRIIFINASSTLMGSAALLLISSLLIKIIA

Target ORF information:

RefSeq Version	NM_166232.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecto-5'-nucleotidase 2 (NT5E-2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166232.2

ORF Insert Sequence:

ATGCAGTCAC GTTGGCTTTG CGGGATCTTC GTGATCGTCC TGGCCCATTG GACACGGGCC 
AATCCAATTT TGAGCCATCC CAAAGTGGCC ACCGAGTTCA TCATCCTCCA TAATAATGAT 
ATGCATGCCC GTTTCGATCA GACGAGCGTT AATAGCGGCA CCTGCCCACC GGAGGATGTA 
CATACGAATA AATGCTATGG AGGATTCGCC AGGGTGGCCC ACGAAGTTCG AAAGTATCGT 
AAGGAGGCTC AGGAGGGCGG TACCTCCGTA TTGTACTTAA ATGCTGGTGA TACCTACACT 
GGAACCTCTT GGTTTACTAT TTTCAAGGAC AAAATCGCCA GTGCTTTCCT TAACAAATTG 
AAACCGGATG CTATATCCTT GGGCAATCAT GAGTTTGATG AGAGAGTAGA GGGTCTAATA 
CCCTTCCTCA ACGAGGTCAC CTTTCCTGTA CTCGCCTGTA ATCTGGACCT AAGCAAGGTT 
CCCCAACTGA AGGCCACTTG GCACTTGGCT AACTCCGCCA TTCTGGAGAC CAATGGCACT 
AAGGTCGGTG TCATAGGTTA TTTGACTCCA GATACCAAAA AACTTACTCT CAACATGGAT 
GTGGAGTTCA ACGAGGAGGT GGAGTCCATT AATGTTGAAG CAAAAAAACT GAAGGCACAG 
GGCATCAAAA TCATTATCGC TCTTGGCCAC TCGGGTTATT TGAAGGATCT GGAGATAGCC 
AAGAACTGTC CGGAGGTGGA TATTGTCATT GGTGGCCATA CGAACACCTT TCTCTACACC 
GGTGCCCAGC CGGATGCGGA GCACATAGAT GGACCGTATC CAACGATGGT CAAGCAGAAC 
AGTGGCAAGG AGGTTCCCGT TGTGCAGGCC TATGCGTATA CCAAATATTT GGGAAAACTG 
CATGTACAGT TTGATGCCGA TGGCAACCTC ATCCAGTGGG ATGGATCTCC CATCCTGCTC 
AATGCTTCAG TGGCTCAGGA GCAGGACCTT CTCGATCTAT TGGAGGTCTT TCGTCCGAAT 
GTCACCAGGC TGGAGAAGTC GGTCGTCGGG CACACCAAGG TTCATCTGGA GGGCAACAAG 
GCGGTTTGCC GGGCCGAGGA GTGCAACCTG GGCAATCTGA TAGCCGATGC AATGGTGTTT 
AGTCGCCTGA TGGAGGAGCA GGGCGGTGAT TTCTGGACAG ATGCTGCCAT TTCTATAATG 
CAGGGCGGAG GCATTCGCAG CTCCATTGAA AAGCGATCGG ATGGCGCCAT CACCGACAAC 
GATATCCTTT CCGTTCTGCC CTGGGGTAAT AAGCTCTATA TGGTGCCCAT GACGGGTAGC 
ACTATTCGTC GAGCCTTAGA GCATGGAGCT GCCCTGAGAG GCAAAGATTC GGACGGTGGC 
TTCCTGCAGG TGTCCGGAAT CCGTGTGGTT TTCAATTCGA ACAAGCCCGA GGGTCAGCGA 
GTGGTTTCCG TGCAGGTTCG ATGTGCCGCC TGCCGAGTGC CCACCTATAG TGATCTGAAT 
GATACTGCCA TCTACAATGT CGTCCTGGGA GAGTTCCTGC TAGATGGTGG CGATGGACAT 
GTAATGAGGG ACTCAGCCCA CCAACCACAG CGCCTGCAAA ATAACGATCT CGAGGCGGTA 
TCCCAATATC TGAACCAGCG TGACTATGTC TATCCCGAGA TCGAGGGTCG TATTATTTTC 
ATTAATGCAT CCAGTACACT TATGGGCTCG GCAGCCCTGC TTCTTATCTC TTCCCTTCTG 
ATAAAAATTA TTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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NT5E-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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