lpin1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lpin1
Entrez Gene ID	799099
Full Name	lipin 1
Synonyms	zgc:194552,zgc:194558
General protein information	Preferred Names lipin 1 Names phosphatidate phosphatase LPIN1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005158809.4	XP_005158866.1	phosphatidate phosphatase LPIN1 isoform X1
NM_001044353.1	NP_001037818.1	phosphatidate phosphatase LPIN1
XM_005158809.3	XP_005158866.1	phosphatidate phosphatase LPIN1 isoform X1
XM_005158810.3	XP_005158867.1	phosphatidate phosphatase LPIN1 isoform X2
XM_005158810.4	XP_005158867.1	phosphatidate phosphatase LPIN1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	LPIN1	NP_001248357.1
Rattus norvegicus (Norway rat)	Lpin1	NP_001012111.1
Macaca mulatta (Rhesus monkey)	LOC697682	XP_002808075.1
Gallus gallus (chicken)	LPIN1	XP_004935828.1
Pan troglodytes (chimpanzee)	LPIN1	XP_001160223.2
Mus musculus (house mouse)	Lpin1	NP_056578.2
Canis lupus familiaris (dog)	LPIN1	XP_532878.3
Bos taurus (cattle)	LPIN1	NP_001193085.1
Xenopus tropicalis (tropical clawed frog)	lpin1	NP_001072495.1
Danio rerio (zebrafish)	lpin1	NP_001037818.1

Gene Ontology
Bibliography

Related articles in PubMed

Canonical Wnt Signaling Remodels Lipid Metabolism in Zebrafish Hepatocytes following Ras Oncogenic Insult.
Yao Y, Sun S, Wang J, Fei F, Dong Z, Ke AW, He R, Wang L, Zhang L, Ji MB, Li Q, Yu M, Shi GM, Fan J, Gong Z, Wang X
Cancer research78(19)5548-5560(2018 10)

Comparative Study of Different Diets-Induced NAFLD Models of Zebrafish.
Chen B, Zheng YM, Zhang JP
Frontiers in endocrinology9366(2018)

Distinct Molecular Signature of Murine Fetal Liver and Adult Hematopoietic Stem Cells Identify Novel Regulators of Hematopoietic Stem Cell Function.
Manesia JK, Franch M, Tabas-Madrid D, Nogales-Cadenas R, Vanwelden T, Van Den Bosch E, Xu Z, Pascual-Montano A, Khurana S, Verfaillie CM
Stem cells and development26(8)573-584(2017 04)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Tributyltin promoted hepatic steatosis in zebrafish (Danio rerio) and the molecular pathogenesis involved.
Zhang J, Sun P, Kong T, Yang F, Guan W
Aquatic toxicology (Amsterdam, Netherlands)170208-215(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following lpin1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lpin1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31093	NM_001044353.1 Latest version!	Danio rerio lipin 1 (lpin1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa22629	XM_005158809.4 Latest version!	Danio rerio lipin 1 (lpin1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa22630	XM_005158810.4 Latest version!	Danio rerio lipin 1 (lpin1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa22629	XM_005158809.3	Danio rerio lipin 1 (lpin1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa22630	XM_005158810.3	Danio rerio lipin 1 (lpin1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lpin1 ( NM_001044353.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001037818.1 Danio rerio lipin 1 (lpin1), mRNA.

Price & Availability↑

CloneID	ODa31093
Clone ID Related Accession (Same CDS sequence)	NM_001044353.1
Accession Version	NM_001044353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1547827200000
Organism	Danio rerio(zebrafish)
Product	phosphatidate phosphatase LPIN1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY822039.1. On Aug 25, 2006 this sequence version replaced XM_679712.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY822039.1, BC163248.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAATTACG TGGGTCAGCT GGCCGGTCAG GTTTTCGTGC AGGTGAAGGA GCTGTACAGA 
GGTCTTAATC CTGCCACTCT GTCTGGCTGT ATCGATGTCA TTGTGGTGCG TCAGCCTGAT 
GGCACACTGC AGTGCTCACC GTTTCACGTC CGATTCGGCA AGATGGGTGT GCTGCGCTCC 
CGGGAGAAAG TGGTTGACAT TGAGATAAAT GGGGAGCCGG TTAGTTTACA CATGAAACTG 
GGCGAAAATG GAGAGGCGTT CTTTGTGAAA GAAACAGAAG ATGATGGGGA GATGGTTCCA 
TCCTACTTGG CTACATCGCC CATCATTTCT GAAGGCTCTG CTCTAATGCA GGCCCAAATG 
GGTAAAGGCA CGTCCCGCCT GTCTGAATCT GGAAACGGAA CCACCATTCA AACCCTGGGC 
CCCATGCAGC CCCCTGGTGA CAGTTCAATA ATGAAGAAAA GGAGGAAAAG GAGAAGAAAA 
TCTAAAGTAG ACAGCATGAA GCGTGAGGAC AATGGAGACT CAGAAGACGA GGACATGTTT 
CCCATCGACA TGAGTTCTGA TGATGATGAC ACAGAGGCTT CAGGCAGCAG AGCAATGTCG 
CATGATTTGT TCGCTGAGCA GCTGAAAGGA GCAAGCAACA CATACATGCA GTCTGAAAGG 
AACTGGGCTG GTGTTCAAAG CACCGGCTTG GAGAAGACCT GCTCTTTACC CATTCCCACC 
AATACAGGAT TATCCATCTC CTGCCCTCAA CAATCCGCGA TGTTCCACAG AACAGACAGT 
CCAAATGGCT CCTTGCCATC TACACCTAAG AGTGACTCAG AGTTGTTGGA TAGTCAAAGG 
AACAAAGGAA AACCAGACAA TCCTGAGATG CTGTGGACAT GGGGAGAGCT GCCTCACGCT 
GCTAAGCCTT CATTCCGGCA GCCGCTACCA GAGCCCATGG TGGTCACCCC AAAGTCAATA 
CCGCGATCTG AAAGCACGCA CTTCAGAGCC ATCACTGAGG ATGGGATGGT GGAGTCTCGG 
GGCAGTGTTA TGTATGTGCC TGATATCAGA ACTGAAGAGG CAACATCTAC TGCGGTTGAA 
GAAGTGGAGC CCATCAGTGT AGTCGCTCAT CTGATCACAG AACTCGAGGA CGGCCGTCAC 
AGTCCTGGAG CTCATGCCAT TAGCAAGACG GACTCTCCAT CCAAAAGAAA AGACAAGAGA 
AGCCCACACT TGGGATCAGA TGGAGTATAT CTGGATGACA TCACAGAGTT GGAGCCTGAG 
GTGGCGGCCT TGTACTTTCC AAAAAGTGAT GGAGCAACAT CTTTGAGGAG TGGAGCAGAT 
TCTAGAAGTG CCAATCAGTC TCCTCAGTCA GTGGGCAGTA GTGGAATGGA CAGTGGGGTG 
GAAAGCTTTT CTGACCAGTT AGGAGACTTG CCTCACATTG CGATCTCTCT ATGCGGAGGT 
CTGTCAGAAA ACAGAGAGAT CACTAGAGAG GAATTTGAGG AACGTGCAAT ATCTTATCAG 
GAGTTTGCAG ATAATCCCGC TATCATTGAT GATCCCAACT TGGTTGTAAA GATTGGAACT 
AAGTATTACA ACTGGACCAC AGCCGCTCCT ATTGTACTGG CAATGCAGGT TTTTCAGAAG 
CCCTTGCCAA CGACTACAGT AGAGAACATC ATGAAGGAGA AGATGCCCAA GAAAGGAGGA 
CGCTGGTGGT TCTCGTGGCG CGGAAGAAAC AGCAGCTCCA AATCGGAATC AGCAGCAGAC 
CAGATTGAAT GTGGAGATGA GTCAATAAGA ACAGGATCTG TCAGCAGATT AAAGGATGAA 
TCCTCATCCA GTGAAGATGA CAGTGGGGGT GCTAAACAGA TTGCTGTGAT GAATCAGCCT 
GAAGTTCTCC ATAGCTCTGG AGGCCACTGC TACAGAAAGA CACTTCGTTT GTCCCCAGAA 
CAGCTAGCCA GTCTGAACTT AAAAGATGGC CCCAATGATG TGGTTTTCAG CGTCACCACC 
CAATACCAAG GCACCTGCCG TTGTGAGGGC ACCATTTATT TGTGGAACTG GGATGACAAA 
ATAGTGATTT CAGATATTGA TGGCACCATC ACAAGGTCTG ACACCCTGGG ACACATTTTA 
CCAACTCTGG GTAAAGACTG GACCCATCAG GGCATTGCCA GACTTTATCA CCGAGTAAGC 
CAGAACGGCT ACAAGTTTAT GTACTGTTCA GCTCGGGCGA TTGGCATGGC TGATATGACC 
CGGGGTTACC TGCACTGGGT TAATGAAAGA GGCACTATGC TACCAATGGG ACCTGTGCTG 
CTTAGTCCTA GCAGTCTATT CTCTGCATTT CACAGGGAGG TCATTGAGAA GAAGCCAGAA 
AAGTTCAAAA TCGAATGTCT GACTGACATT AAAAACTTGT TCTATCCAAA CGCAGAACCC 
TTCTATGCTG CTTTTGGAAA CAGGGCAACA GATGTTTATT CATATAAAGA AGTGGGAGTG 
CCTTTGAACA GAATATTCAC TGTGAATCCT AAGGGAGAGC TCATTCAAGA ACATGCAAAG 
ACCAACATAT CATCTTATGG TCGTCTGTGT GAGGTTGTGG ATCATGTCTT TCCTCTTTTG 
ATCAGAGGGA ACACCACCGA CTTCCCCTGT TCAGACACAT TCAGCCAGTT CACCTTCTCG 
AGAGAACAGT TACCAGAAGT AGAAAAGCAG GATCAACAGG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037818.1
CDS	1..2685
Translation	MNYVGQLAGQVFVQVKELYRGLNPATLSGCIDVIVVRQPDGTLQCSPFHVRFGKMGVLRSREKVVDIEINGEPVSLHMKLGENGEAFFVKETEDDGEMVPSYLATSPIISEGSALMQAQMGKGTSRLSESGNGTTIQTLGPMQPPGDSSIMKKRRKRRRKSKVDSMKREDNGDSEDEDMFPIDMSSDDDDTEASGSRAMSHDLFAEQLKGASNTYMQSERNWAGVQSTGLEKTCSLPIPTNTGLSISCPQQSAMFHRTDSPNGSLPSTPKSDSELLDSQRNKGKPDNPEMLWTWGELPHAAKPSFRQPLPEPMVVTPKSIPRSESTHFRAITEDGMVESRGSVMYVPDIRTEEATSTAVEEVEPISVVAHLITELEDGRHSPGAHAISKTDSPSKRKDKRSPHLGSDGVYLDDITELEPEVAALYFPKSDGATSLRSGADSRSANQSPQSVGSSGMDSGVESFSDQLGDLPHIAISLCGGLSENREITREEFEERAISYQEFADNPAIIDDPNLVVKIGTKYYNWTTAAPIVLAMQVFQKPLPTTTVENIMKEKMPKKGGRWWFSWRGRNSSSKSESAADQIECGDESIRTGSVSRLKDESSSSEDDSGGAKQIAVMNQPEVLHSSGGHCYRKTLRLSPEQLASLNLKDGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKIVISDIDGTITRSDTLGHILPTLGKDWTHQGIARLYHRVSQNGYKFMYCSARAIGMADMTRGYLHWVNERGTMLPMGPVLLSPSSLFSAFHREVIEKKPEKFKIECLTDIKNLFYPNAEPFYAAFGNRATDVYSYKEVGVPLNRIFTVNPKGELIQEHAKTNISSYGRLCEVVDHVFPLLIRGNTTDFPCSDTFSQFTFSREQLPEVEKQDQQA

Target ORF information:

RefSeq Version	NM_001044353.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lipin 1 (lpin1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044353.1

ORF Insert Sequence:

ATGAATTACG TGGGTCAGCT GGCCGGTCAG GTTTTCGTGC AGGTGAAGGA GCTGTACAGA 
GGTCTTAATC CTGCCACTCT GTCTGGCTGT ATCGATGTCA TTGTGGTGCG TCAGCCTGAT 
GGCACACTGC AGTGCTCACC GTTTCACGTC CGATTCGGCA AGATGGGTGT GCTGCGCTCC 
CGGGAGAAAG TGGTTGACAT TGAGATAAAT GGGGAGCCGG TTAGTTTACA CATGAAACTG 
GGCGAAAATG GAGAGGCGTT CTTTGTGAAA GAAACAGAAG ATGATGGGGA GATGGTTCCA 
TCCTACTTGG CTACATCGCC CATCATTTCT GAAGGCTCTG CTCTAATGCA GGCCCAAATG 
GGTAAAGGCA CGTCCCGCCT GTCTGAATCT GGAAACGGAA CCACCATTCA AACCCTGGGC 
CCCATGCAGC CCCCTGGTGA CAGTTCAATA ATGAAGAAAA GGAGGAAAAG GAGAAGAAAA 
TCTAAAGTAG ACAGCATGAA GCGTGAGGAC AATGGAGACT CAGAAGACGA GGACATGTTT 
CCCATCGACA TGAGTTCTGA TGATGATGAC ACAGAGGCTT CAGGCAGCAG AGCAATGTCG 
CATGATTTGT TCGCTGAGCA GCTGAAAGGA GCAAGCAACA CATACATGCA GTCTGAAAGG 
AACTGGGCTG GTGTTCAAAG CACCGGCTTG GAGAAGACCT GCTCTTTACC CATTCCCACC 
AATACAGGAT TATCCATCTC CTGCCCTCAA CAATCCGCGA TGTTCCACAG AACAGACAGT 
CCAAATGGCT CCTTGCCATC TACACCTAAG AGTGACTCAG AGTTGTTGGA TAGTCAAAGG 
AACAAAGGAA AACCAGACAA TCCTGAGATG CTGTGGACAT GGGGAGAGCT GCCTCACGCT 
GCTAAGCCTT CATTCCGGCA GCCGCTACCA GAGCCCATGG TGGTCACCCC AAAGTCAATA 
CCGCGATCTG AAAGCACGCA CTTCAGAGCC ATCACTGAGG ATGGGATGGT GGAGTCTCGG 
GGCAGTGTTA TGTATGTGCC TGATATCAGA ACTGAAGAGG CAACATCTAC TGCGGTTGAA 
GAAGTGGAGC CCATCAGTGT AGTCGCTCAT CTGATCACAG AACTCGAGGA CGGCCGTCAC 
AGTCCTGGAG CTCATGCCAT TAGCAAGACG GACTCTCCAT CCAAAAGAAA AGACAAGAGA 
AGCCCACACT TGGGATCAGA TGGAGTATAT CTGGATGACA TCACAGAGTT GGAGCCTGAG 
GTGGCGGCCT TGTACTTTCC AAAAAGTGAT GGAGCAACAT CTTTGAGGAG TGGAGCAGAT 
TCTAGAAGTG CCAATCAGTC TCCTCAGTCA GTGGGCAGTA GTGGAATGGA CAGTGGGGTG 
GAAAGCTTTT CTGACCAGTT AGGAGACTTG CCTCACATTG CGATCTCTCT ATGCGGAGGT 
CTGTCAGAAA ACAGAGAGAT CACTAGAGAG GAATTTGAGG AACGTGCAAT ATCTTATCAG 
GAGTTTGCAG ATAATCCCGC TATCATTGAT GATCCCAACT TGGTTGTAAA GATTGGAACT 
AAGTATTACA ACTGGACCAC AGCCGCTCCT ATTGTACTGG CAATGCAGGT TTTTCAGAAG 
CCCTTGCCAA CGACTACAGT AGAGAACATC ATGAAGGAGA AGATGCCCAA GAAAGGAGGA 
CGCTGGTGGT TCTCGTGGCG CGGAAGAAAC AGCAGCTCCA AATCGGAATC AGCAGCAGAC 
CAGATTGAAT GTGGAGATGA GTCAATAAGA ACAGGATCTG TCAGCAGATT AAAGGATGAA 
TCCTCATCCA GTGAAGATGA CAGTGGGGGT GCTAAACAGA TTGCTGTGAT GAATCAGCCT 
GAAGTTCTCC ATAGCTCTGG AGGCCACTGC TACAGAAAGA CACTTCGTTT GTCCCCAGAA 
CAGCTAGCCA GTCTGAACTT AAAAGATGGC CCCAATGATG TGGTTTTCAG CGTCACCACC 
CAATACCAAG GCACCTGCCG TTGTGAGGGC ACCATTTATT TGTGGAACTG GGATGACAAA 
ATAGTGATTT CAGATATTGA TGGCACCATC ACAAGGTCTG ACACCCTGGG ACACATTTTA 
CCAACTCTGG GTAAAGACTG GACCCATCAG GGCATTGCCA GACTTTATCA CCGAGTAAGC 
CAGAACGGCT ACAAGTTTAT GTACTGTTCA GCTCGGGCGA TTGGCATGGC TGATATGACC 
CGGGGTTACC TGCACTGGGT TAATGAAAGA GGCACTATGC TACCAATGGG ACCTGTGCTG 
CTTAGTCCTA GCAGTCTATT CTCTGCATTT CACAGGGAGG TCATTGAGAA GAAGCCAGAA 
AAGTTCAAAA TCGAATGTCT GACTGACATT AAAAACTTGT TCTATCCAAA CGCAGAACCC 
TTCTATGCTG CTTTTGGAAA CAGGGCAACA GATGTTTATT CATATAAAGA AGTGGGAGTG 
CCTTTGAACA GAATATTCAC TGTGAATCCT AAGGGAGAGC TCATTCAAGA ACATGCAAAG 
ACCAACATAT CATCTTATGG TCGTCTGTGT GAGGTTGTGG ATCATGTCTT TCCTCTTTTG 
ATCAGAGGGA ACACCACCGA CTTCCCCTGT TCAGACACAT TCAGCCAGTT CACCTTCTCG 
AGAGAACAGT TACCAGAAGT AGAAAAGCAG GATCAACAGG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lpin1 ( XM_005158809.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158866.1 Danio rerio lipin 1 (lpin1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22629
Clone ID Related Accession (Same CDS sequence)	XM_005158809.4 , XM_005158809.3
Accession Version	XM_005158809.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	phosphatidate phosphatase LPIN1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158809.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGCAG AGGATCAAGT GGACGAATAC GATGAGAGAT TTGGCCCAAA TGAGACGAGC 
TGGACATGGA GTCAGACCAT GAATTACGTG GGTCAGCTGG CCGGTCAGGT TTTCGTGCAG 
GTGAAGGAGC TGTACAGAGG TCTTAATCCT GCCACTCTGT CTGGCTGTAT CGATGTCATT 
GTGGTGCGTC AGCCTGATGG CACACTGCAG TGCTCACCGT TTCACGTCCG ATTCGGCAAG 
ATGGGTGTGC TGCGCTCCCG GGAGAAAGTG GTTGACATTG AGATAAATGG GGAGCCGGTT 
AGTTTACACA TGAAACTGGG CGAAAATGGA GAGGCGTTCT TTGTGAAAGA AACAGAAGAT 
GATGGGGAGA TGGTTCCATC CTACTTGGCT ACATCGCCCA TCATTTCTGA AGGCTCTGCT 
CTAATGCAGG CCCAAATGGG TAAAGGCACG TCCCGCCTGT CTGAATCTGG AAACGGAACC 
ACCATTCAAA CCCTGGGCCC CATGCAGCCC CCTGGTGACA GTTCAATAAT GAAGAAAAGG 
AGGAAAAGGA GAAGAAAATC TAAAGTAGAC AGCATGAAGC GTGAGGACAA TGGAGACTCA 
GAAGACGAGG ACATGTTTCC CATCGACATG AGTTCTGATG ATGATGACAC AGAGGCTTCA 
GGCAGCAGAG CAATGTCGCA TGATTTGTTC GCTGAGCAGC TGAAAGGAGC AAGCAACACA 
TACATGCAGT CTGAAAGGAA CTGGGCTGGT GTTCAAAGCA CCGGCTTGGA GAAGACCTGC 
TCTTTACCCA TTCCCACCAA TACAGGATTA TCCATCTCCT GCCCTCAACA ATCCGCGATG 
TTCCACAGAA CAGACAGTCC AAATGGCTCC TTGCCATCTA CACCTAAGAG TGACTCAGAG 
TTGTTGGATA GTCAAAGGAA CAAAGGAAAA CCAGACAATC CTGAGATGCT GTGGACATGG 
GGAGAGCTGC CTCACGCTGC TAAGCCTTCA TTCCGGCAGC CGCTACCAGA GCCCATGGTG 
GTCACCCCAA AGTCAATACC GCGATCTGAA AGCACGCACT TCAGAGCCAT CACTGGGGAT 
GGGATGGTGG AGTCTCGGGG CAGTGTTATG TATGTGCCTG ATATCAGAAC TGAAGAGGCA 
ACATCTACTG CGGTTGAAGA AGTGGAGCCC ATCAGTGTAG TCGCTCATCT GATCACAGAA 
CTCGAGGACG GCCGTCACAG TCCTGGAGCT CATGCCATTA GCAAGACGGA CTCTCCATCC 
AAAAGAAAAG ACAAGAGAAG CCCACACTTG GGATCAGATG GAGTATATCT GGATGACATC 
ACAGAGTTGG AGCCTGAGGT GGCGGCCTTG TACTTTCCAA AAAGTGATGG AGCAACATCT 
TTGAGGAGTG GAGCAGATTC TAGAAGTGCC AATCAGTCTC CTCAGTCAGT GGGCAGTAGT 
GGAATGGACA GTGGGGTGGA AAGCTTTTCT GACCAGTTAG GAGACTTGCC TCACATTGCG 
ATCTCTCTAT GCGGAGGTCT GTCAGAAAAC AGAGAGATCA CTAGAGAGGA ATTTGAGGAA 
CGTGCAATAT CTTATCAGGA GTTTGCAGAT AATCCCGCTA TCATTGATGA TCCCAACTTG 
GTTGTAAAGA TTGGAACTAA GTATTACAAC TGGACCACAG CCGCTCCTAT TGTACTGGCA 
ATGCAGGTTT TTCAGAAGCC CTTGCCAACG GCTACAGTAG AGAACATCAT GAAGGAGAAG 
ATGCCCAAGA AAGGAGGACG CTGGTGGTTC TCGTGGCGCG GAAGAAACAG CAGCTCCAAA 
TCGGAATCAG CAGCAGACCA GATTGAATGT GGAGATGAGT CAATAAGAAC AGGATCTGTC 
AGCAGATTAA AGGATGAATC CTCATCCAGT GAAGATGACA GTGGGGGTGC TAAACAGATT 
GCTGTGATGA ATCAGCCTGA AGTTCTCCAT AGCTCTGGAG GCCACTGCTA CAGAAAGACA 
CTTCGTTTGT CCCCAGAACA GCTAGCCAGT CTGAACTTAA AAGATGGCCC CAATGATGTG 
GTTTTCAGCG TCACCACCCA ATACCAAGGC ACCTGCCGTT GTGAGGGCAC CATTTATTTG 
TGGAACTGGG ATGACAAAAT AGTGATTTCA GATATTGATG GCACCATCAC AAGGTCTGAC 
ACCCTGGGAC ACATTTTACC AACTCTGGGA AAAGACTGGA CCCATCAGGG CATTGCCAGA 
CTTTATCACC GAGTAAGCCA GAACGGCTAC AAGTTTATGT ACTGTTCAGC TCGGGCGATT 
GGCATGGCTG ATATGACCCG GGGTTACCTG CACTGGGTTA ATGAAAGAGG CACTATGCTA 
CCAATGGGAC CTGTGCTGCT TAGTCCTAGC AGTCTATTCT CTGCATTTCA CAGGGAGGTC 
ATTGAGAAGA AGCCAGAAAA GTTCAAAATC GAATGTCTGA CTGACATTAA AAACTTGTTC 
TATCCAAACG CAGAACCCTT CTATGCTGCT TTTGGAAACA GGGCAACAGA TGTTTATTCA 
TATAAAGAAG TGGGAGTGCC TTTGAACAGA ATATTCACTG TGAATCCTAA GGGAGAGCTC 
ATTCAAGAAC ATGCAAAGAC CAACATATCA TCTTATGGTC GTCTGTGTGA GGTTGTGGAT 
CATGTCTTTC CTCTTCTGAT CAGAGGGAAC ACCACCGACT TCCCCTGTTC AGACACATTC 
AGCCAGTTCA CCTTCTGGAG AGAACAGTTA CCAGAAGTAG AAAAGCAGGA TCAACAGGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158866.1
CDS	76..2838
Translation	MIAEDQVDEYDERFGPNETSWTWSQTMNYVGQLAGQVFVQVKELYRGLNPATLSGCIDVIVVRQPDGTLQCSPFHVRFGKMGVLRSREKVVDIEINGEPVSLHMKLGENGEAFFVKETEDDGEMVPSYLATSPIISEGSALMQAQMGKGTSRLSESGNGTTIQTLGPMQPPGDSSIMKKRRKRRRKSKVDSMKREDNGDSEDEDMFPIDMSSDDDDTEASGSRAMSHDLFAEQLKGASNTYMQSERNWAGVQSTGLEKTCSLPIPTNTGLSISCPQQSAMFHRTDSPNGSLPSTPKSDSELLDSQRNKGKPDNPEMLWTWGELPHAAKPSFRQPLPEPMVVTPKSIPRSESTHFRAITGDGMVESRGSVMYVPDIRTEEATSTAVEEVEPISVVAHLITELEDGRHSPGAHAISKTDSPSKRKDKRSPHLGSDGVYLDDITELEPEVAALYFPKSDGATSLRSGADSRSANQSPQSVGSSGMDSGVESFSDQLGDLPHIAISLCGGLSENREITREEFEERAISYQEFADNPAIIDDPNLVVKIGTKYYNWTTAAPIVLAMQVFQKPLPTATVENIMKEKMPKKGGRWWFSWRGRNSSSKSESAADQIECGDESIRTGSVSRLKDESSSSEDDSGGAKQIAVMNQPEVLHSSGGHCYRKTLRLSPEQLASLNLKDGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKIVISDIDGTITRSDTLGHILPTLGKDWTHQGIARLYHRVSQNGYKFMYCSARAIGMADMTRGYLHWVNERGTMLPMGPVLLSPSSLFSAFHREVIEKKPEKFKIECLTDIKNLFYPNAEPFYAAFGNRATDVYSYKEVGVPLNRIFTVNPKGELIQEHAKTNISSYGRLCEVVDHVFPLLIRGNTTDFPCSDTFSQFTFWREQLPEVEKQDQQA

Target ORF information:

RefSeq Version	XM_005158809.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lipin 1 (lpin1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158809.4

ORF Insert Sequence:

ATGATAGCAG AGGATCAAGT GGACGAATAC GATGAGAGAT TTGGCCCAAA TGAGACGAGC 
TGGACATGGA GTCAGACCAT GAATTACGTG GGTCAGCTGG CCGGTCAGGT TTTCGTGCAG 
GTGAAGGAGC TGTACAGAGG TCTTAATCCT GCCACTCTGT CTGGCTGTAT CGATGTCATT 
GTGGTGCGTC AGCCTGATGG CACACTGCAG TGCTCACCGT TTCACGTCCG ATTCGGCAAG 
ATGGGTGTGC TGCGCTCCCG GGAGAAAGTG GTTGACATTG AGATAAATGG GGAGCCGGTT 
AGTTTACACA TGAAACTGGG CGAAAATGGA GAGGCGTTCT TTGTGAAAGA AACAGAAGAT 
GATGGGGAGA TGGTTCCATC CTACTTGGCT ACATCGCCCA TCATTTCTGA AGGCTCTGCT 
CTAATGCAGG CCCAAATGGG TAAAGGCACG TCCCGCCTGT CTGAATCTGG AAACGGAACC 
ACCATTCAAA CCCTGGGCCC CATGCAGCCC CCTGGTGACA GTTCAATAAT GAAGAAAAGG 
AGGAAAAGGA GAAGAAAATC TAAAGTAGAC AGCATGAAGC GTGAGGACAA TGGAGACTCA 
GAAGACGAGG ACATGTTTCC CATCGACATG AGTTCTGATG ATGATGACAC AGAGGCTTCA 
GGCAGCAGAG CAATGTCGCA TGATTTGTTC GCTGAGCAGC TGAAAGGAGC AAGCAACACA 
TACATGCAGT CTGAAAGGAA CTGGGCTGGT GTTCAAAGCA CCGGCTTGGA GAAGACCTGC 
TCTTTACCCA TTCCCACCAA TACAGGATTA TCCATCTCCT GCCCTCAACA ATCCGCGATG 
TTCCACAGAA CAGACAGTCC AAATGGCTCC TTGCCATCTA CACCTAAGAG TGACTCAGAG 
TTGTTGGATA GTCAAAGGAA CAAAGGAAAA CCAGACAATC CTGAGATGCT GTGGACATGG 
GGAGAGCTGC CTCACGCTGC TAAGCCTTCA TTCCGGCAGC CGCTACCAGA GCCCATGGTG 
GTCACCCCAA AGTCAATACC GCGATCTGAA AGCACGCACT TCAGAGCCAT CACTGGGGAT 
GGGATGGTGG AGTCTCGGGG CAGTGTTATG TATGTGCCTG ATATCAGAAC TGAAGAGGCA 
ACATCTACTG CGGTTGAAGA AGTGGAGCCC ATCAGTGTAG TCGCTCATCT GATCACAGAA 
CTCGAGGACG GCCGTCACAG TCCTGGAGCT CATGCCATTA GCAAGACGGA CTCTCCATCC 
AAAAGAAAAG ACAAGAGAAG CCCACACTTG GGATCAGATG GAGTATATCT GGATGACATC 
ACAGAGTTGG AGCCTGAGGT GGCGGCCTTG TACTTTCCAA AAAGTGATGG AGCAACATCT 
TTGAGGAGTG GAGCAGATTC TAGAAGTGCC AATCAGTCTC CTCAGTCAGT GGGCAGTAGT 
GGAATGGACA GTGGGGTGGA AAGCTTTTCT GACCAGTTAG GAGACTTGCC TCACATTGCG 
ATCTCTCTAT GCGGAGGTCT GTCAGAAAAC AGAGAGATCA CTAGAGAGGA ATTTGAGGAA 
CGTGCAATAT CTTATCAGGA GTTTGCAGAT AATCCCGCTA TCATTGATGA TCCCAACTTG 
GTTGTAAAGA TTGGAACTAA GTATTACAAC TGGACCACAG CCGCTCCTAT TGTACTGGCA 
ATGCAGGTTT TTCAGAAGCC CTTGCCAACG GCTACAGTAG AGAACATCAT GAAGGAGAAG 
ATGCCCAAGA AAGGAGGACG CTGGTGGTTC TCGTGGCGCG GAAGAAACAG CAGCTCCAAA 
TCGGAATCAG CAGCAGACCA GATTGAATGT GGAGATGAGT CAATAAGAAC AGGATCTGTC 
AGCAGATTAA AGGATGAATC CTCATCCAGT GAAGATGACA GTGGGGGTGC TAAACAGATT 
GCTGTGATGA ATCAGCCTGA AGTTCTCCAT AGCTCTGGAG GCCACTGCTA CAGAAAGACA 
CTTCGTTTGT CCCCAGAACA GCTAGCCAGT CTGAACTTAA AAGATGGCCC CAATGATGTG 
GTTTTCAGCG TCACCACCCA ATACCAAGGC ACCTGCCGTT GTGAGGGCAC CATTTATTTG 
TGGAACTGGG ATGACAAAAT AGTGATTTCA GATATTGATG GCACCATCAC AAGGTCTGAC 
ACCCTGGGAC ACATTTTACC AACTCTGGGA AAAGACTGGA CCCATCAGGG CATTGCCAGA 
CTTTATCACC GAGTAAGCCA GAACGGCTAC AAGTTTATGT ACTGTTCAGC TCGGGCGATT 
GGCATGGCTG ATATGACCCG GGGTTACCTG CACTGGGTTA ATGAAAGAGG CACTATGCTA 
CCAATGGGAC CTGTGCTGCT TAGTCCTAGC AGTCTATTCT CTGCATTTCA CAGGGAGGTC 
ATTGAGAAGA AGCCAGAAAA GTTCAAAATC GAATGTCTGA CTGACATTAA AAACTTGTTC 
TATCCAAACG CAGAACCCTT CTATGCTGCT TTTGGAAACA GGGCAACAGA TGTTTATTCA 
TATAAAGAAG TGGGAGTGCC TTTGAACAGA ATATTCACTG TGAATCCTAA GGGAGAGCTC 
ATTCAAGAAC ATGCAAAGAC CAACATATCA TCTTATGGTC GTCTGTGTGA GGTTGTGGAT 
CATGTCTTTC CTCTTCTGAT CAGAGGGAAC ACCACCGACT TCCCCTGTTC AGACACATTC 
AGCCAGTTCA CCTTCTGGAG AGAACAGTTA CCAGAAGTAG AAAAGCAGGA TCAACAGGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lpin1 ( XM_005158809.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158866.1 Danio rerio lipin 1 (lpin1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22629
Clone ID Related Accession (Same CDS sequence)	XM_005158809.4 , XM_005158809.3
Accession Version	XM_005158809.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	phosphatidate phosphatase LPIN1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGCAG AGGATCAAGT GGACGAATAC GATGAGAGAT TTGGCCCAAA TGAGACGAGC 
TGGACATGGA GTCAGACCAT GAATTACGTG GGTCAGCTGG CCGGTCAGGT TTTCGTGCAG 
GTGAAGGAGC TGTACAGAGG TCTTAATCCT GCCACTCTGT CTGGCTGTAT CGATGTCATT 
GTGGTGCGTC AGCCTGATGG CACACTGCAG TGCTCACCGT TTCACGTCCG ATTCGGCAAG 
ATGGGTGTGC TGCGCTCCCG GGAGAAAGTG GTTGACATTG AGATAAATGG GGAGCCGGTT 
AGTTTACACA TGAAACTGGG CGAAAATGGA GAGGCGTTCT TTGTGAAAGA AACAGAAGAT 
GATGGGGAGA TGGTTCCATC CTACTTGGCT ACATCGCCCA TCATTTCTGA AGGCTCTGCT 
CTAATGCAGG CCCAAATGGG TAAAGGCACG TCCCGCCTGT CTGAATCTGG AAACGGAACC 
ACCATTCAAA CCCTGGGCCC CATGCAGCCC CCTGGTGACA GTTCAATAAT GAAGAAAAGG 
AGGAAAAGGA GAAGAAAATC TAAAGTAGAC AGCATGAAGC GTGAGGACAA TGGAGACTCA 
GAAGACGAGG ACATGTTTCC CATCGACATG AGTTCTGATG ATGATGACAC AGAGGCTTCA 
GGCAGCAGAG CAATGTCGCA TGATTTGTTC GCTGAGCAGC TGAAAGGAGC AAGCAACACA 
TACATGCAGT CTGAAAGGAA CTGGGCTGGT GTTCAAAGCA CCGGCTTGGA GAAGACCTGC 
TCTTTACCCA TTCCCACCAA TACAGGATTA TCCATCTCCT GCCCTCAACA ATCCGCGATG 
TTCCACAGAA CAGACAGTCC AAATGGCTCC TTGCCATCTA CACCTAAGAG TGACTCAGAG 
TTGTTGGATA GTCAAAGGAA CAAAGGAAAA CCAGACAATC CTGAGATGCT GTGGACATGG 
GGAGAGCTGC CTCACGCTGC TAAGCCTTCA TTCCGGCAGC CGCTACCAGA GCCCATGGTG 
GTCACCCCAA AGTCAATACC GCGATCTGAA AGCACGCACT TCAGAGCCAT CACTGGGGAT 
GGGATGGTGG AGTCTCGGGG CAGTGTTATG TATGTGCCTG ATATCAGAAC TGAAGAGGCA 
ACATCTACTG CGGTTGAAGA AGTGGAGCCC ATCAGTGTAG TCGCTCATCT GATCACAGAA 
CTCGAGGACG GCCGTCACAG TCCTGGAGCT CATGCCATTA GCAAGACGGA CTCTCCATCC 
AAAAGAAAAG ACAAGAGAAG CCCACACTTG GGATCAGATG GAGTATATCT GGATGACATC 
ACAGAGTTGG AGCCTGAGGT GGCGGCCTTG TACTTTCCAA AAAGTGATGG AGCAACATCT 
TTGAGGAGTG GAGCAGATTC TAGAAGTGCC AATCAGTCTC CTCAGTCAGT GGGCAGTAGT 
GGAATGGACA GTGGGGTGGA AAGCTTTTCT GACCAGTTAG GAGACTTGCC TCACATTGCG 
ATCTCTCTAT GCGGAGGTCT GTCAGAAAAC AGAGAGATCA CTAGAGAGGA ATTTGAGGAA 
CGTGCAATAT CTTATCAGGA GTTTGCAGAT AATCCCGCTA TCATTGATGA TCCCAACTTG 
GTTGTAAAGA TTGGAACTAA GTATTACAAC TGGACCACAG CCGCTCCTAT TGTACTGGCA 
ATGCAGGTTT TTCAGAAGCC CTTGCCAACG GCTACAGTAG AGAACATCAT GAAGGAGAAG 
ATGCCCAAGA AAGGAGGACG CTGGTGGTTC TCGTGGCGCG GAAGAAACAG CAGCTCCAAA 
TCGGAATCAG CAGCAGACCA GATTGAATGT GGAGATGAGT CAATAAGAAC AGGATCTGTC 
AGCAGATTAA AGGATGAATC CTCATCCAGT GAAGATGACA GTGGGGGTGC TAAACAGATT 
GCTGTGATGA ATCAGCCTGA AGTTCTCCAT AGCTCTGGAG GCCACTGCTA CAGAAAGACA 
CTTCGTTTGT CCCCAGAACA GCTAGCCAGT CTGAACTTAA AAGATGGCCC CAATGATGTG 
GTTTTCAGCG TCACCACCCA ATACCAAGGC ACCTGCCGTT GTGAGGGCAC CATTTATTTG 
TGGAACTGGG ATGACAAAAT AGTGATTTCA GATATTGATG GCACCATCAC AAGGTCTGAC 
ACCCTGGGAC ACATTTTACC AACTCTGGGA AAAGACTGGA CCCATCAGGG CATTGCCAGA 
CTTTATCACC GAGTAAGCCA GAACGGCTAC AAGTTTATGT ACTGTTCAGC TCGGGCGATT 
GGCATGGCTG ATATGACCCG GGGTTACCTG CACTGGGTTA ATGAAAGAGG CACTATGCTA 
CCAATGGGAC CTGTGCTGCT TAGTCCTAGC AGTCTATTCT CTGCATTTCA CAGGGAGGTC 
ATTGAGAAGA AGCCAGAAAA GTTCAAAATC GAATGTCTGA CTGACATTAA AAACTTGTTC 
TATCCAAACG CAGAACCCTT CTATGCTGCT TTTGGAAACA GGGCAACAGA TGTTTATTCA 
TATAAAGAAG TGGGAGTGCC TTTGAACAGA ATATTCACTG TGAATCCTAA GGGAGAGCTC 
ATTCAAGAAC ATGCAAAGAC CAACATATCA TCTTATGGTC GTCTGTGTGA GGTTGTGGAT 
CATGTCTTTC CTCTTCTGAT CAGAGGGAAC ACCACCGACT TCCCCTGTTC AGACACATTC 
AGCCAGTTCA CCTTCTGGAG AGAACAGTTA CCAGAAGTAG AAAAGCAGGA TCAACAGGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158866.1
CDS	98..2860
Translation	MIAEDQVDEYDERFGPNETSWTWSQTMNYVGQLAGQVFVQVKELYRGLNPATLSGCIDVIVVRQPDGTLQCSPFHVRFGKMGVLRSREKVVDIEINGEPVSLHMKLGENGEAFFVKETEDDGEMVPSYLATSPIISEGSALMQAQMGKGTSRLSESGNGTTIQTLGPMQPPGDSSIMKKRRKRRRKSKVDSMKREDNGDSEDEDMFPIDMSSDDDDTEASGSRAMSHDLFAEQLKGASNTYMQSERNWAGVQSTGLEKTCSLPIPTNTGLSISCPQQSAMFHRTDSPNGSLPSTPKSDSELLDSQRNKGKPDNPEMLWTWGELPHAAKPSFRQPLPEPMVVTPKSIPRSESTHFRAITGDGMVESRGSVMYVPDIRTEEATSTAVEEVEPISVVAHLITELEDGRHSPGAHAISKTDSPSKRKDKRSPHLGSDGVYLDDITELEPEVAALYFPKSDGATSLRSGADSRSANQSPQSVGSSGMDSGVESFSDQLGDLPHIAISLCGGLSENREITREEFEERAISYQEFADNPAIIDDPNLVVKIGTKYYNWTTAAPIVLAMQVFQKPLPTATVENIMKEKMPKKGGRWWFSWRGRNSSSKSESAADQIECGDESIRTGSVSRLKDESSSSEDDSGGAKQIAVMNQPEVLHSSGGHCYRKTLRLSPEQLASLNLKDGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKIVISDIDGTITRSDTLGHILPTLGKDWTHQGIARLYHRVSQNGYKFMYCSARAIGMADMTRGYLHWVNERGTMLPMGPVLLSPSSLFSAFHREVIEKKPEKFKIECLTDIKNLFYPNAEPFYAAFGNRATDVYSYKEVGVPLNRIFTVNPKGELIQEHAKTNISSYGRLCEVVDHVFPLLIRGNTTDFPCSDTFSQFTFWREQLPEVEKQDQQA

Target ORF information:

RefSeq Version	XM_005158809.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lipin 1 (lpin1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158809.3

ORF Insert Sequence:

ATGATAGCAG AGGATCAAGT GGACGAATAC GATGAGAGAT TTGGCCCAAA TGAGACGAGC 
TGGACATGGA GTCAGACCAT GAATTACGTG GGTCAGCTGG CCGGTCAGGT TTTCGTGCAG 
GTGAAGGAGC TGTACAGAGG TCTTAATCCT GCCACTCTGT CTGGCTGTAT CGATGTCATT 
GTGGTGCGTC AGCCTGATGG CACACTGCAG TGCTCACCGT TTCACGTCCG ATTCGGCAAG 
ATGGGTGTGC TGCGCTCCCG GGAGAAAGTG GTTGACATTG AGATAAATGG GGAGCCGGTT 
AGTTTACACA TGAAACTGGG CGAAAATGGA GAGGCGTTCT TTGTGAAAGA AACAGAAGAT 
GATGGGGAGA TGGTTCCATC CTACTTGGCT ACATCGCCCA TCATTTCTGA AGGCTCTGCT 
CTAATGCAGG CCCAAATGGG TAAAGGCACG TCCCGCCTGT CTGAATCTGG AAACGGAACC 
ACCATTCAAA CCCTGGGCCC CATGCAGCCC CCTGGTGACA GTTCAATAAT GAAGAAAAGG 
AGGAAAAGGA GAAGAAAATC TAAAGTAGAC AGCATGAAGC GTGAGGACAA TGGAGACTCA 
GAAGACGAGG ACATGTTTCC CATCGACATG AGTTCTGATG ATGATGACAC AGAGGCTTCA 
GGCAGCAGAG CAATGTCGCA TGATTTGTTC GCTGAGCAGC TGAAAGGAGC AAGCAACACA 
TACATGCAGT CTGAAAGGAA CTGGGCTGGT GTTCAAAGCA CCGGCTTGGA GAAGACCTGC 
TCTTTACCCA TTCCCACCAA TACAGGATTA TCCATCTCCT GCCCTCAACA ATCCGCGATG 
TTCCACAGAA CAGACAGTCC AAATGGCTCC TTGCCATCTA CACCTAAGAG TGACTCAGAG 
TTGTTGGATA GTCAAAGGAA CAAAGGAAAA CCAGACAATC CTGAGATGCT GTGGACATGG 
GGAGAGCTGC CTCACGCTGC TAAGCCTTCA TTCCGGCAGC CGCTACCAGA GCCCATGGTG 
GTCACCCCAA AGTCAATACC GCGATCTGAA AGCACGCACT TCAGAGCCAT CACTGGGGAT 
GGGATGGTGG AGTCTCGGGG CAGTGTTATG TATGTGCCTG ATATCAGAAC TGAAGAGGCA 
ACATCTACTG CGGTTGAAGA AGTGGAGCCC ATCAGTGTAG TCGCTCATCT GATCACAGAA 
CTCGAGGACG GCCGTCACAG TCCTGGAGCT CATGCCATTA GCAAGACGGA CTCTCCATCC 
AAAAGAAAAG ACAAGAGAAG CCCACACTTG GGATCAGATG GAGTATATCT GGATGACATC 
ACAGAGTTGG AGCCTGAGGT GGCGGCCTTG TACTTTCCAA AAAGTGATGG AGCAACATCT 
TTGAGGAGTG GAGCAGATTC TAGAAGTGCC AATCAGTCTC CTCAGTCAGT GGGCAGTAGT 
GGAATGGACA GTGGGGTGGA AAGCTTTTCT GACCAGTTAG GAGACTTGCC TCACATTGCG 
ATCTCTCTAT GCGGAGGTCT GTCAGAAAAC AGAGAGATCA CTAGAGAGGA ATTTGAGGAA 
CGTGCAATAT CTTATCAGGA GTTTGCAGAT AATCCCGCTA TCATTGATGA TCCCAACTTG 
GTTGTAAAGA TTGGAACTAA GTATTACAAC TGGACCACAG CCGCTCCTAT TGTACTGGCA 
ATGCAGGTTT TTCAGAAGCC CTTGCCAACG GCTACAGTAG AGAACATCAT GAAGGAGAAG 
ATGCCCAAGA AAGGAGGACG CTGGTGGTTC TCGTGGCGCG GAAGAAACAG CAGCTCCAAA 
TCGGAATCAG CAGCAGACCA GATTGAATGT GGAGATGAGT CAATAAGAAC AGGATCTGTC 
AGCAGATTAA AGGATGAATC CTCATCCAGT GAAGATGACA GTGGGGGTGC TAAACAGATT 
GCTGTGATGA ATCAGCCTGA AGTTCTCCAT AGCTCTGGAG GCCACTGCTA CAGAAAGACA 
CTTCGTTTGT CCCCAGAACA GCTAGCCAGT CTGAACTTAA AAGATGGCCC CAATGATGTG 
GTTTTCAGCG TCACCACCCA ATACCAAGGC ACCTGCCGTT GTGAGGGCAC CATTTATTTG 
TGGAACTGGG ATGACAAAAT AGTGATTTCA GATATTGATG GCACCATCAC AAGGTCTGAC 
ACCCTGGGAC ACATTTTACC AACTCTGGGA AAAGACTGGA CCCATCAGGG CATTGCCAGA 
CTTTATCACC GAGTAAGCCA GAACGGCTAC AAGTTTATGT ACTGTTCAGC TCGGGCGATT 
GGCATGGCTG ATATGACCCG GGGTTACCTG CACTGGGTTA ATGAAAGAGG CACTATGCTA 
CCAATGGGAC CTGTGCTGCT TAGTCCTAGC AGTCTATTCT CTGCATTTCA CAGGGAGGTC 
ATTGAGAAGA AGCCAGAAAA GTTCAAAATC GAATGTCTGA CTGACATTAA AAACTTGTTC 
TATCCAAACG CAGAACCCTT CTATGCTGCT TTTGGAAACA GGGCAACAGA TGTTTATTCA 
TATAAAGAAG TGGGAGTGCC TTTGAACAGA ATATTCACTG TGAATCCTAA GGGAGAGCTC 
ATTCAAGAAC ATGCAAAGAC CAACATATCA TCTTATGGTC GTCTGTGTGA GGTTGTGGAT 
CATGTCTTTC CTCTTCTGAT CAGAGGGAAC ACCACCGACT TCCCCTGTTC AGACACATTC 
AGCCAGTTCA CCTTCTGGAG AGAACAGTTA CCAGAAGTAG AAAAGCAGGA TCAACAGGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lpin1 ( XM_005158810.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158867.1 Danio rerio lipin 1 (lpin1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa22630
Clone ID Related Accession (Same CDS sequence)	XM_005158810.3 , XM_005158810.4
Accession Version	XM_005158810.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	phosphatidate phosphatase LPIN1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158810.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTACG TGGGTCAGCT GGCCGGTCAG GTTTTCGTGC AGGTGAAGGA GCTGTACAGA 
GGTCTTAATC CTGCCACTCT GTCTGGCTGT ATCGATGTCA TTGTGGTGCG TCAGCCTGAT 
GGCACACTGC AGTGCTCACC GTTTCACGTC CGATTCGGCA AGATGGGTGT GCTGCGCTCC 
CGGGAGAAAG TGGTTGACAT TGAGATAAAT GGGGAGCCGG TTAGTTTACA CATGAAACTG 
GGCGAAAATG GAGAGGCGTT CTTTGTGAAA GAAACAGAAG ATGATGGGGA GATGGTTCCA 
TCCTACTTGG CTACATCGCC CATCATTTCT GAAGGCTCTG CTCTAATGCA GGCCCAAATG 
GGTAAAGGCA CGTCCCGCCT GTCTGAATCT GGAAACGGAA CCACCATTCA AACCCTGGGC 
CCCATGCAGC CCCCTGGTGA CAGTTCAATA ATGAAGAAAA GGAGGAAAAG GAGAAGAAAA 
TCTAAAGTAG ACAGCATGAA GCGTGAGGAC AATGGAGACT CAGAAGACGA GGACATGTTT 
CCCATCGACA TGAGTTCTGA TGATGATGAC ACAGAGGCTT CAGGCAGCAG AGCAATGTCG 
CATGATTTGT TCGCTGAGCA GCTGAAAGGA GCAAGCAACA CATACATGCA GTCTGAAAGG 
AACTGGGCTG GTGTTCAAAG CACCGGCTTG GAGAAGACCT GCTCTTTACC CATTCCCACC 
AATACAGGAT TATCCATCTC CTGCCCTCAA CAATCCGCGA TGTTCCACAG AACAGACAGT 
CCAAATGGCT CCTTGCCATC TACACCTAAG AGTGACTCAG AGTTGTTGGA TAGTCAAAGG 
AACAAAGGAA AACCAGACAA TCCTGAGATG CTGTGGACAT GGGGAGAGCT GCCTCACGCT 
GCTAAGCCTT CATTCCGGCA GCCGCTACCA GAGCCCATGG TGGTCACCCC AAAGTCAATA 
CCGCGATCTG AAAGCACGCA CTTCAGAGCC ATCACTGGGG ATGGGATGGT GGAGTCTCGG 
GGCAGTGTTA TGTATGTGCC TGATATCAGA ACTGAAGAGG CAACATCTAC TGCGGTTGAA 
GAAGTGGAGC CCATCAGTGT AGTCGCTCAT CTGATCACAG AACTCGAGGA CGGCCGTCAC 
AGTCCTGGAG CTCATGCCAT TAGCAAGACG GACTCTCCAT CCAAAAGAAA AGACAAGAGA 
AGCCCACACT TGGGATCAGA TGGAGTATAT CTGGATGACA TCACAGAGTT GGAGCCTGAG 
GTGGCGGCCT TGTACTTTCC AAAAAGTGAT GGAGCAACAT CTTTGAGGAG TGGAGCAGAT 
TCTAGAAGTG CCAATCAGTC TCCTCAGTCA GTGGGCAGTA GTGGAATGGA CAGTGGGGTG 
GAAAGCTTTT CTGACCAGTT AGGAGACTTG CCTCACATTG CGATCTCTCT ATGCGGAGGT 
CTGTCAGAAA ACAGAGAGAT CACTAGAGAG GAATTTGAGG AACGTGCAAT ATCTTATCAG 
GAGTTTGCAG ATAATCCCGC TATCATTGAT GATCCCAACT TGGTTGTAAA GATTGGAACT 
AAGTATTACA ACTGGACCAC AGCCGCTCCT ATTGTACTGG CAATGCAGGT TTTTCAGAAG 
CCCTTGCCAA CGGCTACAGT AGAGAACATC ATGAAGGAGA AGATGCCCAA GAAAGGAGGA 
CGCTGGTGGT TCTCGTGGCG CGGAAGAAAC AGCAGCTCCA AATCGGAATC AGCAGCAGAC 
CAGATTGAAT GTGGAGATGA GTCAATAAGA ACAGGATCTG TCAGCAGATT AAAGGATGAA 
TCCTCATCCA GTGAAGATGA CAGTGGGGGT GCTAAACAGA TTGCTGTGAT GAATCAGCCT 
GAAGTTCTCC ATAGCTCTGG AGGCCACTGC TACAGAAAGA CACTTCGTTT GTCCCCAGAA 
CAGCTAGCCA GTCTGAACTT AAAAGATGGC CCCAATGATG TGGTTTTCAG CGTCACCACC 
CAATACCAAG GCACCTGCCG TTGTGAGGGC ACCATTTATT TGTGGAACTG GGATGACAAA 
ATAGTGATTT CAGATATTGA TGGCACCATC ACAAGGTCTG ACACCCTGGG ACACATTTTA 
CCAACTCTGG GAAAAGACTG GACCCATCAG GGCATTGCCA GACTTTATCA CCGAGTAAGC 
CAGAACGGCT ACAAGTTTAT GTACTGTTCA GCTCGGGCGA TTGGCATGGC TGATATGACC 
CGGGGTTACC TGCACTGGGT TAATGAAAGA GGCACTATGC TACCAATGGG ACCTGTGCTG 
CTTAGTCCTA GCAGTCTATT CTCTGCATTT CACAGGGAGG TCATTGAGAA GAAGCCAGAA 
AAGTTCAAAA TCGAATGTCT GACTGACATT AAAAACTTGT TCTATCCAAA CGCAGAACCC 
TTCTATGCTG CTTTTGGAAA CAGGGCAACA GATGTTTATT CATATAAAGA AGTGGGAGTG 
CCTTTGAACA GAATATTCAC TGTGAATCCT AAGGGAGAGC TCATTCAAGA ACATGCAAAG 
ACCAACATAT CATCTTATGG TCGTCTGTGT GAGGTTGTGG ATCATGTCTT TCCTCTTCTG 
ATCAGAGGGA ACACCACCGA CTTCCCCTGT TCAGACACAT TCAGCCAGTT CACCTTCTGG 
AGAGAACAGT TACCAGAAGT AGAAAAGCAG GATCAACAGG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158867.1
CDS	286..2970
Translation	MNYVGQLAGQVFVQVKELYRGLNPATLSGCIDVIVVRQPDGTLQCSPFHVRFGKMGVLRSREKVVDIEINGEPVSLHMKLGENGEAFFVKETEDDGEMVPSYLATSPIISEGSALMQAQMGKGTSRLSESGNGTTIQTLGPMQPPGDSSIMKKRRKRRRKSKVDSMKREDNGDSEDEDMFPIDMSSDDDDTEASGSRAMSHDLFAEQLKGASNTYMQSERNWAGVQSTGLEKTCSLPIPTNTGLSISCPQQSAMFHRTDSPNGSLPSTPKSDSELLDSQRNKGKPDNPEMLWTWGELPHAAKPSFRQPLPEPMVVTPKSIPRSESTHFRAITGDGMVESRGSVMYVPDIRTEEATSTAVEEVEPISVVAHLITELEDGRHSPGAHAISKTDSPSKRKDKRSPHLGSDGVYLDDITELEPEVAALYFPKSDGATSLRSGADSRSANQSPQSVGSSGMDSGVESFSDQLGDLPHIAISLCGGLSENREITREEFEERAISYQEFADNPAIIDDPNLVVKIGTKYYNWTTAAPIVLAMQVFQKPLPTATVENIMKEKMPKKGGRWWFSWRGRNSSSKSESAADQIECGDESIRTGSVSRLKDESSSSEDDSGGAKQIAVMNQPEVLHSSGGHCYRKTLRLSPEQLASLNLKDGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKIVISDIDGTITRSDTLGHILPTLGKDWTHQGIARLYHRVSQNGYKFMYCSARAIGMADMTRGYLHWVNERGTMLPMGPVLLSPSSLFSAFHREVIEKKPEKFKIECLTDIKNLFYPNAEPFYAAFGNRATDVYSYKEVGVPLNRIFTVNPKGELIQEHAKTNISSYGRLCEVVDHVFPLLIRGNTTDFPCSDTFSQFTFWREQLPEVEKQDQQA

Target ORF information:

RefSeq Version	XM_005158810.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lipin 1 (lpin1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158810.3

ORF Insert Sequence:

ATGAATTACG TGGGTCAGCT GGCCGGTCAG GTTTTCGTGC AGGTGAAGGA GCTGTACAGA 
GGTCTTAATC CTGCCACTCT GTCTGGCTGT ATCGATGTCA TTGTGGTGCG TCAGCCTGAT 
GGCACACTGC AGTGCTCACC GTTTCACGTC CGATTCGGCA AGATGGGTGT GCTGCGCTCC 
CGGGAGAAAG TGGTTGACAT TGAGATAAAT GGGGAGCCGG TTAGTTTACA CATGAAACTG 
GGCGAAAATG GAGAGGCGTT CTTTGTGAAA GAAACAGAAG ATGATGGGGA GATGGTTCCA 
TCCTACTTGG CTACATCGCC CATCATTTCT GAAGGCTCTG CTCTAATGCA GGCCCAAATG 
GGTAAAGGCA CGTCCCGCCT GTCTGAATCT GGAAACGGAA CCACCATTCA AACCCTGGGC 
CCCATGCAGC CCCCTGGTGA CAGTTCAATA ATGAAGAAAA GGAGGAAAAG GAGAAGAAAA 
TCTAAAGTAG ACAGCATGAA GCGTGAGGAC AATGGAGACT CAGAAGACGA GGACATGTTT 
CCCATCGACA TGAGTTCTGA TGATGATGAC ACAGAGGCTT CAGGCAGCAG AGCAATGTCG 
CATGATTTGT TCGCTGAGCA GCTGAAAGGA GCAAGCAACA CATACATGCA GTCTGAAAGG 
AACTGGGCTG GTGTTCAAAG CACCGGCTTG GAGAAGACCT GCTCTTTACC CATTCCCACC 
AATACAGGAT TATCCATCTC CTGCCCTCAA CAATCCGCGA TGTTCCACAG AACAGACAGT 
CCAAATGGCT CCTTGCCATC TACACCTAAG AGTGACTCAG AGTTGTTGGA TAGTCAAAGG 
AACAAAGGAA AACCAGACAA TCCTGAGATG CTGTGGACAT GGGGAGAGCT GCCTCACGCT 
GCTAAGCCTT CATTCCGGCA GCCGCTACCA GAGCCCATGG TGGTCACCCC AAAGTCAATA 
CCGCGATCTG AAAGCACGCA CTTCAGAGCC ATCACTGGGG ATGGGATGGT GGAGTCTCGG 
GGCAGTGTTA TGTATGTGCC TGATATCAGA ACTGAAGAGG CAACATCTAC TGCGGTTGAA 
GAAGTGGAGC CCATCAGTGT AGTCGCTCAT CTGATCACAG AACTCGAGGA CGGCCGTCAC 
AGTCCTGGAG CTCATGCCAT TAGCAAGACG GACTCTCCAT CCAAAAGAAA AGACAAGAGA 
AGCCCACACT TGGGATCAGA TGGAGTATAT CTGGATGACA TCACAGAGTT GGAGCCTGAG 
GTGGCGGCCT TGTACTTTCC AAAAAGTGAT GGAGCAACAT CTTTGAGGAG TGGAGCAGAT 
TCTAGAAGTG CCAATCAGTC TCCTCAGTCA GTGGGCAGTA GTGGAATGGA CAGTGGGGTG 
GAAAGCTTTT CTGACCAGTT AGGAGACTTG CCTCACATTG CGATCTCTCT ATGCGGAGGT 
CTGTCAGAAA ACAGAGAGAT CACTAGAGAG GAATTTGAGG AACGTGCAAT ATCTTATCAG 
GAGTTTGCAG ATAATCCCGC TATCATTGAT GATCCCAACT TGGTTGTAAA GATTGGAACT 
AAGTATTACA ACTGGACCAC AGCCGCTCCT ATTGTACTGG CAATGCAGGT TTTTCAGAAG 
CCCTTGCCAA CGGCTACAGT AGAGAACATC ATGAAGGAGA AGATGCCCAA GAAAGGAGGA 
CGCTGGTGGT TCTCGTGGCG CGGAAGAAAC AGCAGCTCCA AATCGGAATC AGCAGCAGAC 
CAGATTGAAT GTGGAGATGA GTCAATAAGA ACAGGATCTG TCAGCAGATT AAAGGATGAA 
TCCTCATCCA GTGAAGATGA CAGTGGGGGT GCTAAACAGA TTGCTGTGAT GAATCAGCCT 
GAAGTTCTCC ATAGCTCTGG AGGCCACTGC TACAGAAAGA CACTTCGTTT GTCCCCAGAA 
CAGCTAGCCA GTCTGAACTT AAAAGATGGC CCCAATGATG TGGTTTTCAG CGTCACCACC 
CAATACCAAG GCACCTGCCG TTGTGAGGGC ACCATTTATT TGTGGAACTG GGATGACAAA 
ATAGTGATTT CAGATATTGA TGGCACCATC ACAAGGTCTG ACACCCTGGG ACACATTTTA 
CCAACTCTGG GAAAAGACTG GACCCATCAG GGCATTGCCA GACTTTATCA CCGAGTAAGC 
CAGAACGGCT ACAAGTTTAT GTACTGTTCA GCTCGGGCGA TTGGCATGGC TGATATGACC 
CGGGGTTACC TGCACTGGGT TAATGAAAGA GGCACTATGC TACCAATGGG ACCTGTGCTG 
CTTAGTCCTA GCAGTCTATT CTCTGCATTT CACAGGGAGG TCATTGAGAA GAAGCCAGAA 
AAGTTCAAAA TCGAATGTCT GACTGACATT AAAAACTTGT TCTATCCAAA CGCAGAACCC 
TTCTATGCTG CTTTTGGAAA CAGGGCAACA GATGTTTATT CATATAAAGA AGTGGGAGTG 
CCTTTGAACA GAATATTCAC TGTGAATCCT AAGGGAGAGC TCATTCAAGA ACATGCAAAG 
ACCAACATAT CATCTTATGG TCGTCTGTGT GAGGTTGTGG ATCATGTCTT TCCTCTTCTG 
ATCAGAGGGA ACACCACCGA CTTCCCCTGT TCAGACACAT TCAGCCAGTT CACCTTCTGG 
AGAGAACAGT TACCAGAAGT AGAAAAGCAG GATCAACAGG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lpin1 ( XM_005158810.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158867.1 Danio rerio lipin 1 (lpin1), transcript variant X2, mRNA.

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CloneID	ODa22630
Clone ID Related Accession (Same CDS sequence)	XM_005158810.3 , XM_005158810.4
Accession Version	XM_005158810.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	phosphatidate phosphatase LPIN1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158810.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTACG TGGGTCAGCT GGCCGGTCAG GTTTTCGTGC AGGTGAAGGA GCTGTACAGA 
GGTCTTAATC CTGCCACTCT GTCTGGCTGT ATCGATGTCA TTGTGGTGCG TCAGCCTGAT 
GGCACACTGC AGTGCTCACC GTTTCACGTC CGATTCGGCA AGATGGGTGT GCTGCGCTCC 
CGGGAGAAAG TGGTTGACAT TGAGATAAAT GGGGAGCCGG TTAGTTTACA CATGAAACTG 
GGCGAAAATG GAGAGGCGTT CTTTGTGAAA GAAACAGAAG ATGATGGGGA GATGGTTCCA 
TCCTACTTGG CTACATCGCC CATCATTTCT GAAGGCTCTG CTCTAATGCA GGCCCAAATG 
GGTAAAGGCA CGTCCCGCCT GTCTGAATCT GGAAACGGAA CCACCATTCA AACCCTGGGC 
CCCATGCAGC CCCCTGGTGA CAGTTCAATA ATGAAGAAAA GGAGGAAAAG GAGAAGAAAA 
TCTAAAGTAG ACAGCATGAA GCGTGAGGAC AATGGAGACT CAGAAGACGA GGACATGTTT 
CCCATCGACA TGAGTTCTGA TGATGATGAC ACAGAGGCTT CAGGCAGCAG AGCAATGTCG 
CATGATTTGT TCGCTGAGCA GCTGAAAGGA GCAAGCAACA CATACATGCA GTCTGAAAGG 
AACTGGGCTG GTGTTCAAAG CACCGGCTTG GAGAAGACCT GCTCTTTACC CATTCCCACC 
AATACAGGAT TATCCATCTC CTGCCCTCAA CAATCCGCGA TGTTCCACAG AACAGACAGT 
CCAAATGGCT CCTTGCCATC TACACCTAAG AGTGACTCAG AGTTGTTGGA TAGTCAAAGG 
AACAAAGGAA AACCAGACAA TCCTGAGATG CTGTGGACAT GGGGAGAGCT GCCTCACGCT 
GCTAAGCCTT CATTCCGGCA GCCGCTACCA GAGCCCATGG TGGTCACCCC AAAGTCAATA 
CCGCGATCTG AAAGCACGCA CTTCAGAGCC ATCACTGGGG ATGGGATGGT GGAGTCTCGG 
GGCAGTGTTA TGTATGTGCC TGATATCAGA ACTGAAGAGG CAACATCTAC TGCGGTTGAA 
GAAGTGGAGC CCATCAGTGT AGTCGCTCAT CTGATCACAG AACTCGAGGA CGGCCGTCAC 
AGTCCTGGAG CTCATGCCAT TAGCAAGACG GACTCTCCAT CCAAAAGAAA AGACAAGAGA 
AGCCCACACT TGGGATCAGA TGGAGTATAT CTGGATGACA TCACAGAGTT GGAGCCTGAG 
GTGGCGGCCT TGTACTTTCC AAAAAGTGAT GGAGCAACAT CTTTGAGGAG TGGAGCAGAT 
TCTAGAAGTG CCAATCAGTC TCCTCAGTCA GTGGGCAGTA GTGGAATGGA CAGTGGGGTG 
GAAAGCTTTT CTGACCAGTT AGGAGACTTG CCTCACATTG CGATCTCTCT ATGCGGAGGT 
CTGTCAGAAA ACAGAGAGAT CACTAGAGAG GAATTTGAGG AACGTGCAAT ATCTTATCAG 
GAGTTTGCAG ATAATCCCGC TATCATTGAT GATCCCAACT TGGTTGTAAA GATTGGAACT 
AAGTATTACA ACTGGACCAC AGCCGCTCCT ATTGTACTGG CAATGCAGGT TTTTCAGAAG 
CCCTTGCCAA CGGCTACAGT AGAGAACATC ATGAAGGAGA AGATGCCCAA GAAAGGAGGA 
CGCTGGTGGT TCTCGTGGCG CGGAAGAAAC AGCAGCTCCA AATCGGAATC AGCAGCAGAC 
CAGATTGAAT GTGGAGATGA GTCAATAAGA ACAGGATCTG TCAGCAGATT AAAGGATGAA 
TCCTCATCCA GTGAAGATGA CAGTGGGGGT GCTAAACAGA TTGCTGTGAT GAATCAGCCT 
GAAGTTCTCC ATAGCTCTGG AGGCCACTGC TACAGAAAGA CACTTCGTTT GTCCCCAGAA 
CAGCTAGCCA GTCTGAACTT AAAAGATGGC CCCAATGATG TGGTTTTCAG CGTCACCACC 
CAATACCAAG GCACCTGCCG TTGTGAGGGC ACCATTTATT TGTGGAACTG GGATGACAAA 
ATAGTGATTT CAGATATTGA TGGCACCATC ACAAGGTCTG ACACCCTGGG ACACATTTTA 
CCAACTCTGG GAAAAGACTG GACCCATCAG GGCATTGCCA GACTTTATCA CCGAGTAAGC 
CAGAACGGCT ACAAGTTTAT GTACTGTTCA GCTCGGGCGA TTGGCATGGC TGATATGACC 
CGGGGTTACC TGCACTGGGT TAATGAAAGA GGCACTATGC TACCAATGGG ACCTGTGCTG 
CTTAGTCCTA GCAGTCTATT CTCTGCATTT CACAGGGAGG TCATTGAGAA GAAGCCAGAA 
AAGTTCAAAA TCGAATGTCT GACTGACATT AAAAACTTGT TCTATCCAAA CGCAGAACCC 
TTCTATGCTG CTTTTGGAAA CAGGGCAACA GATGTTTATT CATATAAAGA AGTGGGAGTG 
CCTTTGAACA GAATATTCAC TGTGAATCCT AAGGGAGAGC TCATTCAAGA ACATGCAAAG 
ACCAACATAT CATCTTATGG TCGTCTGTGT GAGGTTGTGG ATCATGTCTT TCCTCTTCTG 
ATCAGAGGGA ACACCACCGA CTTCCCCTGT TCAGACACAT TCAGCCAGTT CACCTTCTGG 
AGAGAACAGT TACCAGAAGT AGAAAAGCAG GATCAACAGG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158867.1
CDS	533..3217
Translation	MNYVGQLAGQVFVQVKELYRGLNPATLSGCIDVIVVRQPDGTLQCSPFHVRFGKMGVLRSREKVVDIEINGEPVSLHMKLGENGEAFFVKETEDDGEMVPSYLATSPIISEGSALMQAQMGKGTSRLSESGNGTTIQTLGPMQPPGDSSIMKKRRKRRRKSKVDSMKREDNGDSEDEDMFPIDMSSDDDDTEASGSRAMSHDLFAEQLKGASNTYMQSERNWAGVQSTGLEKTCSLPIPTNTGLSISCPQQSAMFHRTDSPNGSLPSTPKSDSELLDSQRNKGKPDNPEMLWTWGELPHAAKPSFRQPLPEPMVVTPKSIPRSESTHFRAITGDGMVESRGSVMYVPDIRTEEATSTAVEEVEPISVVAHLITELEDGRHSPGAHAISKTDSPSKRKDKRSPHLGSDGVYLDDITELEPEVAALYFPKSDGATSLRSGADSRSANQSPQSVGSSGMDSGVESFSDQLGDLPHIAISLCGGLSENREITREEFEERAISYQEFADNPAIIDDPNLVVKIGTKYYNWTTAAPIVLAMQVFQKPLPTATVENIMKEKMPKKGGRWWFSWRGRNSSSKSESAADQIECGDESIRTGSVSRLKDESSSSEDDSGGAKQIAVMNQPEVLHSSGGHCYRKTLRLSPEQLASLNLKDGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKIVISDIDGTITRSDTLGHILPTLGKDWTHQGIARLYHRVSQNGYKFMYCSARAIGMADMTRGYLHWVNERGTMLPMGPVLLSPSSLFSAFHREVIEKKPEKFKIECLTDIKNLFYPNAEPFYAAFGNRATDVYSYKEVGVPLNRIFTVNPKGELIQEHAKTNISSYGRLCEVVDHVFPLLIRGNTTDFPCSDTFSQFTFWREQLPEVEKQDQQA

Target ORF information:

RefSeq Version	XM_005158810.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lipin 1 (lpin1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158810.4

ORF Insert Sequence:

ATGAATTACG TGGGTCAGCT GGCCGGTCAG GTTTTCGTGC AGGTGAAGGA GCTGTACAGA 
GGTCTTAATC CTGCCACTCT GTCTGGCTGT ATCGATGTCA TTGTGGTGCG TCAGCCTGAT 
GGCACACTGC AGTGCTCACC GTTTCACGTC CGATTCGGCA AGATGGGTGT GCTGCGCTCC 
CGGGAGAAAG TGGTTGACAT TGAGATAAAT GGGGAGCCGG TTAGTTTACA CATGAAACTG 
GGCGAAAATG GAGAGGCGTT CTTTGTGAAA GAAACAGAAG ATGATGGGGA GATGGTTCCA 
TCCTACTTGG CTACATCGCC CATCATTTCT GAAGGCTCTG CTCTAATGCA GGCCCAAATG 
GGTAAAGGCA CGTCCCGCCT GTCTGAATCT GGAAACGGAA CCACCATTCA AACCCTGGGC 
CCCATGCAGC CCCCTGGTGA CAGTTCAATA ATGAAGAAAA GGAGGAAAAG GAGAAGAAAA 
TCTAAAGTAG ACAGCATGAA GCGTGAGGAC AATGGAGACT CAGAAGACGA GGACATGTTT 
CCCATCGACA TGAGTTCTGA TGATGATGAC ACAGAGGCTT CAGGCAGCAG AGCAATGTCG 
CATGATTTGT TCGCTGAGCA GCTGAAAGGA GCAAGCAACA CATACATGCA GTCTGAAAGG 
AACTGGGCTG GTGTTCAAAG CACCGGCTTG GAGAAGACCT GCTCTTTACC CATTCCCACC 
AATACAGGAT TATCCATCTC CTGCCCTCAA CAATCCGCGA TGTTCCACAG AACAGACAGT 
CCAAATGGCT CCTTGCCATC TACACCTAAG AGTGACTCAG AGTTGTTGGA TAGTCAAAGG 
AACAAAGGAA AACCAGACAA TCCTGAGATG CTGTGGACAT GGGGAGAGCT GCCTCACGCT 
GCTAAGCCTT CATTCCGGCA GCCGCTACCA GAGCCCATGG TGGTCACCCC AAAGTCAATA 
CCGCGATCTG AAAGCACGCA CTTCAGAGCC ATCACTGGGG ATGGGATGGT GGAGTCTCGG 
GGCAGTGTTA TGTATGTGCC TGATATCAGA ACTGAAGAGG CAACATCTAC TGCGGTTGAA 
GAAGTGGAGC CCATCAGTGT AGTCGCTCAT CTGATCACAG AACTCGAGGA CGGCCGTCAC 
AGTCCTGGAG CTCATGCCAT TAGCAAGACG GACTCTCCAT CCAAAAGAAA AGACAAGAGA 
AGCCCACACT TGGGATCAGA TGGAGTATAT CTGGATGACA TCACAGAGTT GGAGCCTGAG 
GTGGCGGCCT TGTACTTTCC AAAAAGTGAT GGAGCAACAT CTTTGAGGAG TGGAGCAGAT 
TCTAGAAGTG CCAATCAGTC TCCTCAGTCA GTGGGCAGTA GTGGAATGGA CAGTGGGGTG 
GAAAGCTTTT CTGACCAGTT AGGAGACTTG CCTCACATTG CGATCTCTCT ATGCGGAGGT 
CTGTCAGAAA ACAGAGAGAT CACTAGAGAG GAATTTGAGG AACGTGCAAT ATCTTATCAG 
GAGTTTGCAG ATAATCCCGC TATCATTGAT GATCCCAACT TGGTTGTAAA GATTGGAACT 
AAGTATTACA ACTGGACCAC AGCCGCTCCT ATTGTACTGG CAATGCAGGT TTTTCAGAAG 
CCCTTGCCAA CGGCTACAGT AGAGAACATC ATGAAGGAGA AGATGCCCAA GAAAGGAGGA 
CGCTGGTGGT TCTCGTGGCG CGGAAGAAAC AGCAGCTCCA AATCGGAATC AGCAGCAGAC 
CAGATTGAAT GTGGAGATGA GTCAATAAGA ACAGGATCTG TCAGCAGATT AAAGGATGAA 
TCCTCATCCA GTGAAGATGA CAGTGGGGGT GCTAAACAGA TTGCTGTGAT GAATCAGCCT 
GAAGTTCTCC ATAGCTCTGG AGGCCACTGC TACAGAAAGA CACTTCGTTT GTCCCCAGAA 
CAGCTAGCCA GTCTGAACTT AAAAGATGGC CCCAATGATG TGGTTTTCAG CGTCACCACC 
CAATACCAAG GCACCTGCCG TTGTGAGGGC ACCATTTATT TGTGGAACTG GGATGACAAA 
ATAGTGATTT CAGATATTGA TGGCACCATC ACAAGGTCTG ACACCCTGGG ACACATTTTA 
CCAACTCTGG GAAAAGACTG GACCCATCAG GGCATTGCCA GACTTTATCA CCGAGTAAGC 
CAGAACGGCT ACAAGTTTAT GTACTGTTCA GCTCGGGCGA TTGGCATGGC TGATATGACC 
CGGGGTTACC TGCACTGGGT TAATGAAAGA GGCACTATGC TACCAATGGG ACCTGTGCTG 
CTTAGTCCTA GCAGTCTATT CTCTGCATTT CACAGGGAGG TCATTGAGAA GAAGCCAGAA 
AAGTTCAAAA TCGAATGTCT GACTGACATT AAAAACTTGT TCTATCCAAA CGCAGAACCC 
TTCTATGCTG CTTTTGGAAA CAGGGCAACA GATGTTTATT CATATAAAGA AGTGGGAGTG 
CCTTTGAACA GAATATTCAC TGTGAATCCT AAGGGAGAGC TCATTCAAGA ACATGCAAAG 
ACCAACATAT CATCTTATGG TCGTCTGTGT GAGGTTGTGG ATCATGTCTT TCCTCTTCTG 
ATCAGAGGGA ACACCACCGA CTTCCCCTGT TCAGACACAT TCAGCCAGTT CACCTTCTGG 
AGAGAACAGT TACCAGAAGT AGAAAAGCAG GATCAACAGG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Canonical Wnt Signaling Remodels Lipid Metabolism in Zebrafish Hepatocytes following Ras Oncogenic Insult.
Cancer research78(19)5548-5560(2018 10)
Yao Y,Sun S,Wang J,Fei F,Dong Z,Ke AW,He R,Wang L,Zhang L,Ji MB,Li Q,Yu M,Shi GM,Fan J,Gong Z,Wang X

Comparative Study of Different Diets-Induced NAFLD Models of Zebrafish.
Frontiers in endocrinology9366(2018)
Chen B,Zheng YM,Zhang JP

Distinct Molecular Signature of Murine Fetal Liver and Adult Hematopoietic Stem Cells Identify Novel Regulators of Hematopoietic Stem Cell Function.
Stem cells and development26(8)573-584(2017 04)
Manesia JK,Franch M,Tabas-Madrid D,Nogales-Cadenas R,Vanwelden T,Van Den Bosch E,Xu Z,Pascual-Montano A,Khurana S,Verfaillie CM

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Tributyltin promoted hepatic steatosis in zebrafish (Danio rerio) and the molecular pathogenesis involved.
Aquatic toxicology (Amsterdam, Netherlands)170208-215(2016 Jan)
Zhang J,Sun P,Kong T,Yang F,Guan W

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lpin1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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