gria1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gria1a
Entrez Gene ID	798689
Full Name	glutamate receptor, ionotropic, AMPA 1a
Synonyms	ZfGluR1a,glur1a,gria1.1,zfGRIA1[a]
General protein information	Preferred Names glutamate receptor, ionotropic, AMPA 1a Names glutamate receptor 1 AMPA receptor subunit GluR1A Gria1b glutamate receptor, ionotropic, AMPA1.1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005173262.3	XP_005173319.1	glutamate receptor 1 isoform X3
XM_005173262.4	XP_005173319.1	glutamate receptor 1 isoform X1
XM_009291147.2	XP_009289422.1	glutamate receptor 1 isoform X2
XM_005173263.3	XP_005173320.1	glutamate receptor 1 isoform X1
NM_205598.1	NP_991161.1	glutamate receptor 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GRIA1	NP_001107655.1
Canis lupus familiaris (dog)	GRIA1	XP_546286.2
Bos taurus (cattle)	GRIA1	XP_002689365.1
Danio rerio (zebrafish)	gria1b	NP_991293.1
Rattus norvegicus (Norway rat)	Gria1	NP_113796.1
Macaca mulatta (Rhesus monkey)	GRIA1	XP_002804636.1
Mus musculus (house mouse)	Gria1	NP_001106796.1
Gallus gallus (chicken)	GRIA1	NP_001001774.1
Danio rerio (zebrafish)	gria1a	NP_991161.1
Pan troglodytes (chimpanzee)	GRIA1	XP_003310973.1
Xenopus tropicalis (tropical clawed frog)	gria1	XP_002941536.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Knockdown of Carboxypeptidase A6 in Zebrafish Larvae Reduces Response to Seizure-Inducing Drugs and Causes Changes in the Level of mRNAs Encoding Signaling Molecules.
Lopes MW, Sapio MR, Leal RB, Fricker LD
PloS one11(4)e0152905(2016)

Growth hormone (GH) increases cognition and expression of ionotropic glutamate receptors (AMPA and NMDA) in transgenic zebrafish (Danio rerio).
Studzinski AL, Barros DM, Marins LF
Behavioural brain research29436-42(2015 Nov)

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
Deng C, Chen H, Yang N, Feng Y, Hsueh AJ
The Journal of biological chemistry290(30)18261-8(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gria1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gria1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa30700	NM_205598.1 Latest version!	Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa19744	XM_005173262.4 Latest version!	Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa19745	XM_005173263.3 Latest version!	Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa47286	XM_009291147.2 Latest version!	Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa19744	XM_005173262.3	Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gria1a ( NM_205598.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991161.1 Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), mRNA.

Price & Availability↑

CloneID	ODa30700
Clone ID Related Accession (Same CDS sequence)	NM_205598.1
Accession Version	NM_205598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1501862400000
Organism	Danio rerio(zebrafish)
Product	glutamate receptor 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF525741.1. On Feb 21, 2008 this sequence version replaced XM_001336429.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF525741.1, BC162505.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCAGCGAA GCCTCACACT GCTCTGCACC AGTTTTCTGG GATTGTGCTT CGGTTCCAGT 
TTCCCAAGTA ATATCAACAT AGGAGGGTTA TTCCCAACCG AATCACACGA ATATGAAGTG 
TTTCGTTTTG CGCTCTCGCA TCACCAGGAT ATCCCTAAAC TGGTACCTCA AGTGGATATG 
GTCAAATTGG GCAATAGCTT CTCCATGACT TATGCGTTTT GTTCGCAGTT CTCCAAAGGA 
GTGTACGCAA TCATTGGCGT CTACGACAGG AAGACCGTCA ACATGTTGAT GTCGTTCTGC 
GGTGCGCTAC ACGTCTGCTT CGTGACGCCC AGCTTCCCTA TGGAAAACTC CAACCAGTTT 
GTCATCCAGC TGAGGCCTGA ACTGCAGGAC GCCCTGGTCG GTGTAATCGA GCACTACAGG 
TGGTCTAAGT TTGTCTACAT GTACAGCTCG GACTCTGGAC TGTCTGTTCT GCAGAAGGTT 
CTGGACACAG CAGCAGAGCA TAACTGGCTG GTGACCTCTG TAAACGTGGA GACAATGACA 
GAGGCTTCCT TTCTCAAAGT GTTCCAGGAT CTGGACAAAA AGAAGGAGGG CCAGATAATT 
ATCGACTGTG AGCTGGAGAG ACTCACCTCC ATTCTGAAGA AGATCATCGA GCTGGGGAAG 
AATGTGAAGA GCTACCACTA CATCCTAGCG AACCTGGGAT TTTTGGACAT TGACCTGACG 
GAGTTTAAGA AGGGTGAGGC GAATGTAACA GGCTTTCAGC TGGTGAATTA CGCAGATCCT 
AATGTCAGCA GGACAGTGCA GCAGTGGATG GAGTTTGACA ATAAAGATGC CAAAGTTCCC 
AAGCGAAGAC TGAAGTACAC TGGAGCTCTC ACATATGATG GGGTCAAAGT CATGTCCACA 
GCTTTCCAGA ACCTCCGCAG GCAGAGGATA GATATATCTC GACGCGGAAA TGCTGGAGAA 
TGTCTGGCCA ACCCACCGGC ACCCTGGGGC CAAGGCATTG ACATTCAGAG AGCGCTTCAA 
CAGGTTCGGA TTGAAGGCTT AACAGGACAC ATTCAGTTTA ACGAGAAAGG ACGAAGGACA 
AACTACTCTA TCAGTGTGAT GGAGCTCAGG CCGACGGGAC CAAAGAAAGT GGGTTACTGG 
AATGAGGATG AGAAATTTGT GAGCACGGCG GCCTACATGC CGGGAAGTAA TGAGACTTAC 
GGACTTCAGA ACCGCACATA TATTGTTACC ACTATTCTGG AATCTCCATA TGTCATGCTG 
AAGAAGAACC ATGAACATTT CACAGGAAAC GATAAGTATG AAGGTTATTG TGTGGAACTG 
GCAGCAGAGA TCGCCAAACA TGTGGGATAC CACTACATAC TCAAGATAGT GGCTGATGGG 
AAATATGGAG CCAGAGATGC GGAGACCATG ATGTGGAATG GGATGGTAGG AGAGCTTGTC 
TATGGTAAAG CTGATGTAGC TGTGGCCCCG CTTACTATCA CCTTGGTCAG AGAGGAAGTC 
ATTGATTTCT CCAAGCCCTT CATGAGCCTG GGCATCTCCA TCATGATCAA AAAACCCACC 
AAATCTAAGC CTGGCGTCTT CTCCTTCCTG GACCCTCTGG CCTATGAGAT CTGGATGTGT 
ATTGTGTTTG CTTACATCGG CGTGAGCGTG GTACTTTTTC TGGTCAGCCG CTTCAGTCCC 
TATGAGTGGC ATGCAGATGA CTGTGAGGAC GGAGCAGAGG AGAACCCAAA CCAGCCGTCC 
TCATCATCCG CCCAACCGGG TCAGAACCAG CAAAACCAGA ACCAGCAGAG TCAGGAGCAA 
ACCAACGAGT TTGGCATCTT CAACAGTCTC TGGTTCTCAC TGGGAGCTTT CATGCAGCAG 
GGCTGTGACA TTTCCCCCAG GTCTCTGTCT GGCCGTATCG TGGGTGGTGT GTGGTGGTTC 
TTCACGCTAA TCATCATCTC CTCGTACACA GCCAACCTTG CTGCATTTCT CACAGTAGAG 
AGGATGGTGT CGCCCATCGA GAGTGCAGAA GATTTGGCCA AACAGACTGA GATTGCTTAC 
GGCACTCTGG ACGCAGGCTC CACCAAAGAG TTCTTCAGGA GGTCAAAGAT TGCAGTCTTT 
GAGAAGATGT GGTCTTACAT GAAGTCAGCA GATCCCTCAG TGTTTGTGAA AACCACAGAT 
GAAGGAGTTA TGCGGGTGAG GAAATCCAAA GGGAAGTATG CATACTTGCT GGAGTCCACC 
ATGAACGAGT ATATCGAGCA GCGCAAACCC TGCGACACTA TGAAGGTTGG AGGGAACCTG 
GACTCCAAAG GCTACGGTAT TGCTACCCCC AAAGGTTCCC CTTTAAGAAA CCCAGTAAAC 
CTGGCAGTGT TAAAACTGAA TGAACAAGGC CTGTTGGACA AATTGAAAAA CAAATGGTGG 
TACGACAAGG GAGAGTGTGG CAGCGGAGGA GGTGATTCCA AGGACAAGAC GAGTGCTCTT 
AGCCTCAGTA ACGTGGCTGG GGTTTTCTAC ATCCTCATCG GCGGTCTGGG TCTGGCCATG 
CTGGTGGCTC TGGTGGAGTT CTGCTATAAA TCCAGGACAG AGTCTCGCAG AATGAAGCAA 
TCCATCAACG ACGCCATGCA CTGCTCCACG CTGACCAGGA TGAGCGGGAA CGGCAGCGGT 
GGGGAGAACG GGCGGATCGT CACACATGAC TTCCCCAAAG CTGTGCAGAC GCTGCCGTGC 
ATGAGCCACG CGGCCAGCAT GGGCCTGGGA GCCTCTGGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991161.1
CDS	114..2858
Translation	MQRSLTLLCTSFLGLCFGSSFPSNINIGGLFPTESHEYEVFRFALSHHQDIPKLVPQVDMVKLGNSFSMTYAFCSQFSKGVYAIIGVYDRKTVNMLMSFCGALHVCFVTPSFPMENSNQFVIQLRPELQDALVGVIEHYRWSKFVYMYSSDSGLSVLQKVLDTAAEHNWLVTSVNVETMTEASFLKVFQDLDKKKEGQIIIDCELERLTSILKKIIELGKNVKSYHYILANLGFLDIDLTEFKKGEANVTGFQLVNYADPNVSRTVQQWMEFDNKDAKVPKRRLKYTGALTYDGVKVMSTAFQNLRRQRIDISRRGNAGECLANPPAPWGQGIDIQRALQQVRIEGLTGHIQFNEKGRRTNYSISVMELRPTGPKKVGYWNEDEKFVSTAAYMPGSNETYGLQNRTYIVTTILESPYVMLKKNHEHFTGNDKYEGYCVELAAEIAKHVGYHYILKIVADGKYGARDAETMMWNGMVGELVYGKADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPTKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHADDCEDGAEENPNQPSSSSAQPGQNQQNQNQQSQEQTNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDAGSTKEFFRRSKIAVFEKMWSYMKSADPSVFVKTTDEGVMRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSPLRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALVEFCYKSRTESRRMKQSINDAMHCSTLTRMSGNGSGGENGRIVTHDFPKAVQTLPCMSHAASMGLGASGM

Target ORF information:

RefSeq Version	NM_205598.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205598.1

ORF Insert Sequence:

ATGCAGCGAA GCCTCACACT GCTCTGCACC AGTTTTCTGG GATTGTGCTT CGGTTCCAGT 
TTCCCAAGTA ATATCAACAT AGGAGGGTTA TTCCCAACCG AATCACACGA ATATGAAGTG 
TTTCGTTTTG CGCTCTCGCA TCACCAGGAT ATCCCTAAAC TGGTACCTCA AGTGGATATG 
GTCAAATTGG GCAATAGCTT CTCCATGACT TATGCGTTTT GTTCGCAGTT CTCCAAAGGA 
GTGTACGCAA TCATTGGCGT CTACGACAGG AAGACCGTCA ACATGTTGAT GTCGTTCTGC 
GGTGCGCTAC ACGTCTGCTT CGTGACGCCC AGCTTCCCTA TGGAAAACTC CAACCAGTTT 
GTCATCCAGC TGAGGCCTGA ACTGCAGGAC GCCCTGGTCG GTGTAATCGA GCACTACAGG 
TGGTCTAAGT TTGTCTACAT GTACAGCTCG GACTCTGGAC TGTCTGTTCT GCAGAAGGTT 
CTGGACACAG CAGCAGAGCA TAACTGGCTG GTGACCTCTG TAAACGTGGA GACAATGACA 
GAGGCTTCCT TTCTCAAAGT GTTCCAGGAT CTGGACAAAA AGAAGGAGGG CCAGATAATT 
ATCGACTGTG AGCTGGAGAG ACTCACCTCC ATTCTGAAGA AGATCATCGA GCTGGGGAAG 
AATGTGAAGA GCTACCACTA CATCCTAGCG AACCTGGGAT TTTTGGACAT TGACCTGACG 
GAGTTTAAGA AGGGTGAGGC GAATGTAACA GGCTTTCAGC TGGTGAATTA CGCAGATCCT 
AATGTCAGCA GGACAGTGCA GCAGTGGATG GAGTTTGACA ATAAAGATGC CAAAGTTCCC 
AAGCGAAGAC TGAAGTACAC TGGAGCTCTC ACATATGATG GGGTCAAAGT CATGTCCACA 
GCTTTCCAGA ACCTCCGCAG GCAGAGGATA GATATATCTC GACGCGGAAA TGCTGGAGAA 
TGTCTGGCCA ACCCACCGGC ACCCTGGGGC CAAGGCATTG ACATTCAGAG AGCGCTTCAA 
CAGGTTCGGA TTGAAGGCTT AACAGGACAC ATTCAGTTTA ACGAGAAAGG ACGAAGGACA 
AACTACTCTA TCAGTGTGAT GGAGCTCAGG CCGACGGGAC CAAAGAAAGT GGGTTACTGG 
AATGAGGATG AGAAATTTGT GAGCACGGCG GCCTACATGC CGGGAAGTAA TGAGACTTAC 
GGACTTCAGA ACCGCACATA TATTGTTACC ACTATTCTGG AATCTCCATA TGTCATGCTG 
AAGAAGAACC ATGAACATTT CACAGGAAAC GATAAGTATG AAGGTTATTG TGTGGAACTG 
GCAGCAGAGA TCGCCAAACA TGTGGGATAC CACTACATAC TCAAGATAGT GGCTGATGGG 
AAATATGGAG CCAGAGATGC GGAGACCATG ATGTGGAATG GGATGGTAGG AGAGCTTGTC 
TATGGTAAAG CTGATGTAGC TGTGGCCCCG CTTACTATCA CCTTGGTCAG AGAGGAAGTC 
ATTGATTTCT CCAAGCCCTT CATGAGCCTG GGCATCTCCA TCATGATCAA AAAACCCACC 
AAATCTAAGC CTGGCGTCTT CTCCTTCCTG GACCCTCTGG CCTATGAGAT CTGGATGTGT 
ATTGTGTTTG CTTACATCGG CGTGAGCGTG GTACTTTTTC TGGTCAGCCG CTTCAGTCCC 
TATGAGTGGC ATGCAGATGA CTGTGAGGAC GGAGCAGAGG AGAACCCAAA CCAGCCGTCC 
TCATCATCCG CCCAACCGGG TCAGAACCAG CAAAACCAGA ACCAGCAGAG TCAGGAGCAA 
ACCAACGAGT TTGGCATCTT CAACAGTCTC TGGTTCTCAC TGGGAGCTTT CATGCAGCAG 
GGCTGTGACA TTTCCCCCAG GTCTCTGTCT GGCCGTATCG TGGGTGGTGT GTGGTGGTTC 
TTCACGCTAA TCATCATCTC CTCGTACACA GCCAACCTTG CTGCATTTCT CACAGTAGAG 
AGGATGGTGT CGCCCATCGA GAGTGCAGAA GATTTGGCCA AACAGACTGA GATTGCTTAC 
GGCACTCTGG ACGCAGGCTC CACCAAAGAG TTCTTCAGGA GGTCAAAGAT TGCAGTCTTT 
GAGAAGATGT GGTCTTACAT GAAGTCAGCA GATCCCTCAG TGTTTGTGAA AACCACAGAT 
GAAGGAGTTA TGCGGGTGAG GAAATCCAAA GGGAAGTATG CATACTTGCT GGAGTCCACC 
ATGAACGAGT ATATCGAGCA GCGCAAACCC TGCGACACTA TGAAGGTTGG AGGGAACCTG 
GACTCCAAAG GCTACGGTAT TGCTACCCCC AAAGGTTCCC CTTTAAGAAA CCCAGTAAAC 
CTGGCAGTGT TAAAACTGAA TGAACAAGGC CTGTTGGACA AATTGAAAAA CAAATGGTGG 
TACGACAAGG GAGAGTGTGG CAGCGGAGGA GGTGATTCCA AGGACAAGAC GAGTGCTCTT 
AGCCTCAGTA ACGTGGCTGG GGTTTTCTAC ATCCTCATCG GCGGTCTGGG TCTGGCCATG 
CTGGTGGCTC TGGTGGAGTT CTGCTATAAA TCCAGGACAG AGTCTCGCAG AATGAAGCAA 
TCCATCAACG ACGCCATGCA CTGCTCCACG CTGACCAGGA TGAGCGGGAA CGGCAGCGGT 
GGGGAGAACG GGCGGATCGT CACACATGAC TTCCCCAAAG CTGTGCAGAC GCTGCCGTGC 
ATGAGCCACG CGGCCAGCAT GGGCCTGGGA GCCTCTGGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gria1a ( XM_005173262.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173319.1 Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa19744
Clone ID Related Accession (Same CDS sequence)	XM_005173262.3 , XM_005173262.4
Accession Version	XM_005173262.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	glutamate receptor 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173262.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGAA GCCTCACACT GCTCTGCACC AGTTTTCTGG GATTGTGCTT CGGTTCCAGT 
TTCCCAAGTA ATATCAACAT AGGAGGGTTA TTCCCAACCG AATCACACGA ATATGAAGTG 
TTTCGTTTTG CGCTCTCGCA TCACCAGGAT ATCCCTAAAC TGGTACCTCA AGTGGATATG 
GTCAAATTGG GCAATAGCTT CTCCATGACT TATGCGTTTT GTTCGCAGTT CTCCAAAGGA 
GTGTACGCAA TCATTGGCGT CTACGACAGG AAGACCGTCA ACATGTTGAT GTCGTTCTGC 
GGTGCGCTAC ACGTCTGCTT CGTGACGCCC AGCTTCCCTA TGGAAAACTC CAACCAGTTT 
GTCATCCAGC TGAGGCCTGA ACTGCAGGAC GCCCTGGTTG GTGTAATCGA GCACTACAGG 
TGGTCTAAGT TTGTCTACAT GTACAGCTCG GACTCTGGAC TGTCTGTTCT GCAGAAGGTT 
CTGGACACAG CAGCAGAGCA TAACTGGCTG GTGACCTCTG TGAACGTGGA GACAATGACA 
GAGGCGTCCT TTCTCAAAGT GTTCCAGGAT CTGGACAAAA AGAAGGAGGG CCAGATAATC 
ATCGACTGTG AGCTGGAGAG ACTCACCTCC ATTCTGAAGA AGATCATCGA GCTGGGGAAG 
AATGTGAAGA GCTACCACTA CATCCTAGCG AACCTGGGAT TTTTGGACAT TGACCTGACG 
GAGTTTAAGA AGGGTGAGGC GAATGTAACA GGCTTTCAGC TGGTGAATTA CGCAGATCCT 
AATGTCAGCA GGACAGTGCA GCAGTGGATG GAGTTTGACA ATAAAGATGC CAAAGTTCCC 
AAGCGAAGAC TGAAGTACAC TGGAGCTCTC ACATATGATG GGGTCAAAGT CATGTCCACA 
GCTTTCCAGA ACCTCCGCAG GCAGAGGATA GATATATCTC GACGCGGAAA TGCTGGAGAA 
TGTCTGGCCA ATCCACCGGC ACCCTGGGGC CAAGGCATTG ACATTCAGAG AGCGCTTCAA 
CAGGTTCGGA TTGAAGGCTT AACAGGACAC ATTCAGTTTA ACGAGAAAGG ACGAAGGACA 
AACTACTCTA TCAGTGTGAT GGAGCTCAGG CCGACGGGAC CAAAGAAAGT GGGTTACTGG 
AATGAGGATG AGAAATTTGT GAGCACGGCG GCCTACATGC CGGGAAGTAA TGAGACTTAC 
GGACTTCAGA ACCGCACATA TATTGTTACC ACTATTCTGG AATCTCCATA TGTCATGCTG 
AAGAAGAACC ATGAACATTT CACAGGAAAC GATAAGTATG AAGGTTATTG TGTGGAACTG 
GCTGCAGAGA TCGCCAAACA TGTGGGATAC CACTACATAC TCAAGATAGT GGCTGATGGG 
AAATATGGAG CCAGAGATGC GGAGACCATG ATGTGGAATG GGATGGTAGG AGAGCTTGTC 
TATGGTAAAG CTGATGTAGC TGTGGCCCCG CTGACTATCA CCTTGGTCAG AGAGGAAGTC 
ATTGATTTCT CCAAGCCCTT CATGAGCCTG GGCATCTCCA TCATGATCAA AAAACCCACC 
AAATCTAAGC CTGGCGTCTT CTCCTTCCTG GACCCTCTGG CCTATGAGAT CTGGATGTGT 
ATTGTGTTTG CTTACATCGG CGTGAGCGTG GTACTTTTTC TGGTCAGCCG CTTCAGTCCC 
TATGAGTGGC ATGCAGATGA CTGTGAGGAC GGAGCAGAGG AGAACCCAAA CCAGCCGTCC 
TCATCATCCG CCCAACCGGG TCAGAACCAG CAAAACCAGA ACCAGCAGAG TCAGGAGCAA 
ACCAACGAGT TTGGCATCTT CAACAGTCTC TGGTTCTCAC TGGGAGCTTT CATGCAGCAG 
GGCTGTGACA TTTCCCCCAG GTCTCTGTCT GGCCGTATCG TGGGTGGTGT GTGGTGGTTC 
TTTACGCTAA TCATCATCTC CTCGTACACA GCCAACCTTG CTGCATTTCT CACAGTAGAG 
AGGATGGTGT CGCCCATCGA GAGTGCAGAA GATTTGGCCA AACAGACTGA GATTGCTTAC 
GGCACTCTGG ACGCAGGCTC CACCAAAGAG TTCTTCAGGA GGTCAAAGAT TGCAGTCTTT 
GAGAAGATGT GGTCTTACAT GAAGTCAGCA GATCCCTCAG TGTTTGTGAA AACCACAGAT 
GAAGGAGTTA TGCGGGTGAG GAAATCCAAA GGGAAGTATG CATACTTGCT GGAGTCCACC 
ATGAACGAGT ATATCGAGCA GCGCAAACCT TGCGACACTA TGAAGGTTGG AGGGAACCTG 
GACTCCAAAG GCTACGGTAT TGCTACCCCC AAAGGTTCCC CTTTAAGAAT CCCAGTTAAC 
CTAGCGGTGT TGAAACTCAG TGAGCAAGCC GTCTTAGACA AACTGAAAAA CAAATGGTGG 
TACGATAAGG GGGAGTGTGG AAGCAAGGAC TCCGGAAGAA AGGACAAGAC GAGTGCTCTT 
AGCCTCAGTA ACGTGGCTGG GGTTTTCTAC ATCCTGATCG GCGGTCTGGG TCTGGCCATG 
CTGGTGGCTC TGGTGGAGTT CTGCTATAAA TCCAGGACAG AGTCTCGCAG AATGAAGCAA 
TCCATCAACG ACGCCATGCA CTGCTCCACG CTGACCAGGA TGAGCGGGAA CGGCAACGGT 
GGGGAGAACG GGCGGATCGT CACACATGAC TTCCCCAAAG CTGTGCAGAC GCTGCCGTGC 
ATGAGCCACG CGGCCAGCAT GGGCCTGGGA GCCTCTGGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173319.1
CDS	221..2965
Translation	MQRSLTLLCTSFLGLCFGSSFPSNINIGGLFPTESHEYEVFRFALSHHQDIPKLVPQVDMVKLGNSFSMTYAFCSQFSKGVYAIIGVYDRKTVNMLMSFCGALHVCFVTPSFPMENSNQFVIQLRPELQDALVGVIEHYRWSKFVYMYSSDSGLSVLQKVLDTAAEHNWLVTSVNVETMTEASFLKVFQDLDKKKEGQIIIDCELERLTSILKKIIELGKNVKSYHYILANLGFLDIDLTEFKKGEANVTGFQLVNYADPNVSRTVQQWMEFDNKDAKVPKRRLKYTGALTYDGVKVMSTAFQNLRRQRIDISRRGNAGECLANPPAPWGQGIDIQRALQQVRIEGLTGHIQFNEKGRRTNYSISVMELRPTGPKKVGYWNEDEKFVSTAAYMPGSNETYGLQNRTYIVTTILESPYVMLKKNHEHFTGNDKYEGYCVELAAEIAKHVGYHYILKIVADGKYGARDAETMMWNGMVGELVYGKADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPTKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHADDCEDGAEENPNQPSSSSAQPGQNQQNQNQQSQEQTNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDAGSTKEFFRRSKIAVFEKMWSYMKSADPSVFVKTTDEGVMRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSPLRIPVNLAVLKLSEQAVLDKLKNKWWYDKGECGSKDSGRKDKTSALSLSNVAGVFYILIGGLGLAMLVALVEFCYKSRTESRRMKQSINDAMHCSTLTRMSGNGNGGENGRIVTHDFPKAVQTLPCMSHAASMGLGASGM

Target ORF information:

RefSeq Version	XM_005173262.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173262.3

ORF Insert Sequence:

ATGCAGCGAA GCCTCACACT GCTCTGCACC AGTTTTCTGG GATTGTGCTT CGGTTCCAGT 
TTCCCAAGTA ATATCAACAT AGGAGGGTTA TTCCCAACCG AATCACACGA ATATGAAGTG 
TTTCGTTTTG CGCTCTCGCA TCACCAGGAT ATCCCTAAAC TGGTACCTCA AGTGGATATG 
GTCAAATTGG GCAATAGCTT CTCCATGACT TATGCGTTTT GTTCGCAGTT CTCCAAAGGA 
GTGTACGCAA TCATTGGCGT CTACGACAGG AAGACCGTCA ACATGTTGAT GTCGTTCTGC 
GGTGCGCTAC ACGTCTGCTT CGTGACGCCC AGCTTCCCTA TGGAAAACTC CAACCAGTTT 
GTCATCCAGC TGAGGCCTGA ACTGCAGGAC GCCCTGGTTG GTGTAATCGA GCACTACAGG 
TGGTCTAAGT TTGTCTACAT GTACAGCTCG GACTCTGGAC TGTCTGTTCT GCAGAAGGTT 
CTGGACACAG CAGCAGAGCA TAACTGGCTG GTGACCTCTG TGAACGTGGA GACAATGACA 
GAGGCGTCCT TTCTCAAAGT GTTCCAGGAT CTGGACAAAA AGAAGGAGGG CCAGATAATC 
ATCGACTGTG AGCTGGAGAG ACTCACCTCC ATTCTGAAGA AGATCATCGA GCTGGGGAAG 
AATGTGAAGA GCTACCACTA CATCCTAGCG AACCTGGGAT TTTTGGACAT TGACCTGACG 
GAGTTTAAGA AGGGTGAGGC GAATGTAACA GGCTTTCAGC TGGTGAATTA CGCAGATCCT 
AATGTCAGCA GGACAGTGCA GCAGTGGATG GAGTTTGACA ATAAAGATGC CAAAGTTCCC 
AAGCGAAGAC TGAAGTACAC TGGAGCTCTC ACATATGATG GGGTCAAAGT CATGTCCACA 
GCTTTCCAGA ACCTCCGCAG GCAGAGGATA GATATATCTC GACGCGGAAA TGCTGGAGAA 
TGTCTGGCCA ATCCACCGGC ACCCTGGGGC CAAGGCATTG ACATTCAGAG AGCGCTTCAA 
CAGGTTCGGA TTGAAGGCTT AACAGGACAC ATTCAGTTTA ACGAGAAAGG ACGAAGGACA 
AACTACTCTA TCAGTGTGAT GGAGCTCAGG CCGACGGGAC CAAAGAAAGT GGGTTACTGG 
AATGAGGATG AGAAATTTGT GAGCACGGCG GCCTACATGC CGGGAAGTAA TGAGACTTAC 
GGACTTCAGA ACCGCACATA TATTGTTACC ACTATTCTGG AATCTCCATA TGTCATGCTG 
AAGAAGAACC ATGAACATTT CACAGGAAAC GATAAGTATG AAGGTTATTG TGTGGAACTG 
GCTGCAGAGA TCGCCAAACA TGTGGGATAC CACTACATAC TCAAGATAGT GGCTGATGGG 
AAATATGGAG CCAGAGATGC GGAGACCATG ATGTGGAATG GGATGGTAGG AGAGCTTGTC 
TATGGTAAAG CTGATGTAGC TGTGGCCCCG CTGACTATCA CCTTGGTCAG AGAGGAAGTC 
ATTGATTTCT CCAAGCCCTT CATGAGCCTG GGCATCTCCA TCATGATCAA AAAACCCACC 
AAATCTAAGC CTGGCGTCTT CTCCTTCCTG GACCCTCTGG CCTATGAGAT CTGGATGTGT 
ATTGTGTTTG CTTACATCGG CGTGAGCGTG GTACTTTTTC TGGTCAGCCG CTTCAGTCCC 
TATGAGTGGC ATGCAGATGA CTGTGAGGAC GGAGCAGAGG AGAACCCAAA CCAGCCGTCC 
TCATCATCCG CCCAACCGGG TCAGAACCAG CAAAACCAGA ACCAGCAGAG TCAGGAGCAA 
ACCAACGAGT TTGGCATCTT CAACAGTCTC TGGTTCTCAC TGGGAGCTTT CATGCAGCAG 
GGCTGTGACA TTTCCCCCAG GTCTCTGTCT GGCCGTATCG TGGGTGGTGT GTGGTGGTTC 
TTTACGCTAA TCATCATCTC CTCGTACACA GCCAACCTTG CTGCATTTCT CACAGTAGAG 
AGGATGGTGT CGCCCATCGA GAGTGCAGAA GATTTGGCCA AACAGACTGA GATTGCTTAC 
GGCACTCTGG ACGCAGGCTC CACCAAAGAG TTCTTCAGGA GGTCAAAGAT TGCAGTCTTT 
GAGAAGATGT GGTCTTACAT GAAGTCAGCA GATCCCTCAG TGTTTGTGAA AACCACAGAT 
GAAGGAGTTA TGCGGGTGAG GAAATCCAAA GGGAAGTATG CATACTTGCT GGAGTCCACC 
ATGAACGAGT ATATCGAGCA GCGCAAACCT TGCGACACTA TGAAGGTTGG AGGGAACCTG 
GACTCCAAAG GCTACGGTAT TGCTACCCCC AAAGGTTCCC CTTTAAGAAT CCCAGTTAAC 
CTAGCGGTGT TGAAACTCAG TGAGCAAGCC GTCTTAGACA AACTGAAAAA CAAATGGTGG 
TACGATAAGG GGGAGTGTGG AAGCAAGGAC TCCGGAAGAA AGGACAAGAC GAGTGCTCTT 
AGCCTCAGTA ACGTGGCTGG GGTTTTCTAC ATCCTGATCG GCGGTCTGGG TCTGGCCATG 
CTGGTGGCTC TGGTGGAGTT CTGCTATAAA TCCAGGACAG AGTCTCGCAG AATGAAGCAA 
TCCATCAACG ACGCCATGCA CTGCTCCACG CTGACCAGGA TGAGCGGGAA CGGCAACGGT 
GGGGAGAACG GGCGGATCGT CACACATGAC TTCCCCAAAG CTGTGCAGAC GCTGCCGTGC 
ATGAGCCACG CGGCCAGCAT GGGCCTGGGA GCCTCTGGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gria1a ( XM_005173262.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173319.1 Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19744
Clone ID Related Accession (Same CDS sequence)	XM_005173262.3 , XM_005173262.4
Accession Version	XM_005173262.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	glutamate receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173262.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGAA GCCTCACACT GCTCTGCACC AGTTTTCTGG GATTGTGCTT CGGTTCCAGT 
TTCCCAAGTA ATATCAACAT AGGAGGGTTA TTCCCAACCG AATCACACGA ATATGAAGTG 
TTTCGTTTTG CGCTCTCGCA TCACCAGGAT ATCCCTAAAC TGGTACCTCA AGTGGATATG 
GTCAAATTGG GCAATAGCTT CTCCATGACT TATGCGTTTT GTTCGCAGTT CTCCAAAGGA 
GTGTACGCAA TCATTGGCGT CTACGACAGG AAGACCGTCA ACATGTTGAT GTCGTTCTGC 
GGTGCGCTAC ACGTCTGCTT CGTGACGCCC AGCTTCCCTA TGGAAAACTC CAACCAGTTT 
GTCATCCAGC TGAGGCCTGA ACTGCAGGAC GCCCTGGTTG GTGTAATCGA GCACTACAGG 
TGGTCTAAGT TTGTCTACAT GTACAGCTCG GACTCTGGAC TGTCTGTTCT GCAGAAGGTT 
CTGGACACAG CAGCAGAGCA TAACTGGCTG GTGACCTCTG TGAACGTGGA GACAATGACA 
GAGGCGTCCT TTCTCAAAGT GTTCCAGGAT CTGGACAAAA AGAAGGAGGG CCAGATAATC 
ATCGACTGTG AGCTGGAGAG ACTCACCTCC ATTCTGAAGA AGATCATCGA GCTGGGGAAG 
AATGTGAAGA GCTACCACTA CATCCTAGCG AACCTGGGAT TTTTGGACAT TGACCTGACG 
GAGTTTAAGA AGGGTGAGGC GAATGTAACA GGCTTTCAGC TGGTGAATTA CGCAGATCCT 
AATGTCAGCA GGACAGTGCA GCAGTGGATG GAGTTTGACA ATAAAGATGC CAAAGTTCCC 
AAGCGAAGAC TGAAGTACAC TGGAGCTCTC ACATATGATG GGGTCAAAGT CATGTCCACA 
GCTTTCCAGA ACCTCCGCAG GCAGAGGATA GATATATCTC GACGCGGAAA TGCTGGAGAA 
TGTCTGGCCA ATCCACCGGC ACCCTGGGGC CAAGGCATTG ACATTCAGAG AGCGCTTCAA 
CAGGTTCGGA TTGAAGGCTT AACAGGACAC ATTCAGTTTA ACGAGAAAGG ACGAAGGACA 
AACTACTCTA TCAGTGTGAT GGAGCTCAGG CCGACGGGAC CAAAGAAAGT GGGTTACTGG 
AATGAGGATG AGAAATTTGT GAGCACGGCG GCCTACATGC CGGGAAGTAA TGAGACTTAC 
GGACTTCAGA ACCGCACATA TATTGTTACC ACTATTCTGG AATCTCCATA TGTCATGCTG 
AAGAAGAACC ATGAACATTT CACAGGAAAC GATAAGTATG AAGGTTATTG TGTGGAACTG 
GCTGCAGAGA TCGCCAAACA TGTGGGATAC CACTACATAC TCAAGATAGT GGCTGATGGG 
AAATATGGAG CCAGAGATGC GGAGACCATG ATGTGGAATG GGATGGTAGG AGAGCTTGTC 
TATGGTAAAG CTGATGTAGC TGTGGCCCCG CTGACTATCA CCTTGGTCAG AGAGGAAGTC 
ATTGATTTCT CCAAGCCCTT CATGAGCCTG GGCATCTCCA TCATGATCAA AAAACCCACC 
AAATCTAAGC CTGGCGTCTT CTCCTTCCTG GACCCTCTGG CCTATGAGAT CTGGATGTGT 
ATTGTGTTTG CTTACATCGG CGTGAGCGTG GTACTTTTTC TGGTCAGCCG CTTCAGTCCC 
TATGAGTGGC ATGCAGATGA CTGTGAGGAC GGAGCAGAGG AGAACCCAAA CCAGCCGTCC 
TCATCATCCG CCCAACCGGG TCAGAACCAG CAAAACCAGA ACCAGCAGAG TCAGGAGCAA 
ACCAACGAGT TTGGCATCTT CAACAGTCTC TGGTTCTCAC TGGGAGCTTT CATGCAGCAG 
GGCTGTGACA TTTCCCCCAG GTCTCTGTCT GGCCGTATCG TGGGTGGTGT GTGGTGGTTC 
TTTACGCTAA TCATCATCTC CTCGTACACA GCCAACCTTG CTGCATTTCT CACAGTAGAG 
AGGATGGTGT CGCCCATCGA GAGTGCAGAA GATTTGGCCA AACAGACTGA GATTGCTTAC 
GGCACTCTGG ACGCAGGCTC CACCAAAGAG TTCTTCAGGA GGTCAAAGAT TGCAGTCTTT 
GAGAAGATGT GGTCTTACAT GAAGTCAGCA GATCCCTCAG TGTTTGTGAA AACCACAGAT 
GAAGGAGTTA TGCGGGTGAG GAAATCCAAA GGGAAGTATG CATACTTGCT GGAGTCCACC 
ATGAACGAGT ATATCGAGCA GCGCAAACCT TGCGACACTA TGAAGGTTGG AGGGAACCTG 
GACTCCAAAG GCTACGGTAT TGCTACCCCC AAAGGTTCCC CTTTAAGAAT CCCAGTTAAC 
CTAGCGGTGT TGAAACTCAG TGAGCAAGCC GTCTTAGACA AACTGAAAAA CAAATGGTGG 
TACGATAAGG GGGAGTGTGG AAGCAAGGAC TCCGGAAGAA AGGACAAGAC GAGTGCTCTT 
AGCCTCAGTA ACGTGGCTGG GGTTTTCTAC ATCCTGATCG GCGGTCTGGG TCTGGCCATG 
CTGGTGGCTC TGGTGGAGTT CTGCTATAAA TCCAGGACAG AGTCTCGCAG AATGAAGCAA 
TCCATCAACG ACGCCATGCA CTGCTCCACG CTGACCAGGA TGAGCGGGAA CGGCAACGGT 
GGGGAGAACG GGCGGATCGT CACACATGAC TTCCCCAAAG CTGTGCAGAC GCTGCCGTGC 
ATGAGCCACG CGGCCAGCAT GGGCCTGGGA GCCTCTGGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173319.1
CDS	212..2956
Translation	MQRSLTLLCTSFLGLCFGSSFPSNINIGGLFPTESHEYEVFRFALSHHQDIPKLVPQVDMVKLGNSFSMTYAFCSQFSKGVYAIIGVYDRKTVNMLMSFCGALHVCFVTPSFPMENSNQFVIQLRPELQDALVGVIEHYRWSKFVYMYSSDSGLSVLQKVLDTAAEHNWLVTSVNVETMTEASFLKVFQDLDKKKEGQIIIDCELERLTSILKKIIELGKNVKSYHYILANLGFLDIDLTEFKKGEANVTGFQLVNYADPNVSRTVQQWMEFDNKDAKVPKRRLKYTGALTYDGVKVMSTAFQNLRRQRIDISRRGNAGECLANPPAPWGQGIDIQRALQQVRIEGLTGHIQFNEKGRRTNYSISVMELRPTGPKKVGYWNEDEKFVSTAAYMPGSNETYGLQNRTYIVTTILESPYVMLKKNHEHFTGNDKYEGYCVELAAEIAKHVGYHYILKIVADGKYGARDAETMMWNGMVGELVYGKADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPTKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHADDCEDGAEENPNQPSSSSAQPGQNQQNQNQQSQEQTNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDAGSTKEFFRRSKIAVFEKMWSYMKSADPSVFVKTTDEGVMRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSPLRIPVNLAVLKLSEQAVLDKLKNKWWYDKGECGSKDSGRKDKTSALSLSNVAGVFYILIGGLGLAMLVALVEFCYKSRTESRRMKQSINDAMHCSTLTRMSGNGNGGENGRIVTHDFPKAVQTLPCMSHAASMGLGASGM

Target ORF information:

RefSeq Version	XM_005173262.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173262.4

ORF Insert Sequence:

ATGCAGCGAA GCCTCACACT GCTCTGCACC AGTTTTCTGG GATTGTGCTT CGGTTCCAGT 
TTCCCAAGTA ATATCAACAT AGGAGGGTTA TTCCCAACCG AATCACACGA ATATGAAGTG 
TTTCGTTTTG CGCTCTCGCA TCACCAGGAT ATCCCTAAAC TGGTACCTCA AGTGGATATG 
GTCAAATTGG GCAATAGCTT CTCCATGACT TATGCGTTTT GTTCGCAGTT CTCCAAAGGA 
GTGTACGCAA TCATTGGCGT CTACGACAGG AAGACCGTCA ACATGTTGAT GTCGTTCTGC 
GGTGCGCTAC ACGTCTGCTT CGTGACGCCC AGCTTCCCTA TGGAAAACTC CAACCAGTTT 
GTCATCCAGC TGAGGCCTGA ACTGCAGGAC GCCCTGGTTG GTGTAATCGA GCACTACAGG 
TGGTCTAAGT TTGTCTACAT GTACAGCTCG GACTCTGGAC TGTCTGTTCT GCAGAAGGTT 
CTGGACACAG CAGCAGAGCA TAACTGGCTG GTGACCTCTG TGAACGTGGA GACAATGACA 
GAGGCGTCCT TTCTCAAAGT GTTCCAGGAT CTGGACAAAA AGAAGGAGGG CCAGATAATC 
ATCGACTGTG AGCTGGAGAG ACTCACCTCC ATTCTGAAGA AGATCATCGA GCTGGGGAAG 
AATGTGAAGA GCTACCACTA CATCCTAGCG AACCTGGGAT TTTTGGACAT TGACCTGACG 
GAGTTTAAGA AGGGTGAGGC GAATGTAACA GGCTTTCAGC TGGTGAATTA CGCAGATCCT 
AATGTCAGCA GGACAGTGCA GCAGTGGATG GAGTTTGACA ATAAAGATGC CAAAGTTCCC 
AAGCGAAGAC TGAAGTACAC TGGAGCTCTC ACATATGATG GGGTCAAAGT CATGTCCACA 
GCTTTCCAGA ACCTCCGCAG GCAGAGGATA GATATATCTC GACGCGGAAA TGCTGGAGAA 
TGTCTGGCCA ATCCACCGGC ACCCTGGGGC CAAGGCATTG ACATTCAGAG AGCGCTTCAA 
CAGGTTCGGA TTGAAGGCTT AACAGGACAC ATTCAGTTTA ACGAGAAAGG ACGAAGGACA 
AACTACTCTA TCAGTGTGAT GGAGCTCAGG CCGACGGGAC CAAAGAAAGT GGGTTACTGG 
AATGAGGATG AGAAATTTGT GAGCACGGCG GCCTACATGC CGGGAAGTAA TGAGACTTAC 
GGACTTCAGA ACCGCACATA TATTGTTACC ACTATTCTGG AATCTCCATA TGTCATGCTG 
AAGAAGAACC ATGAACATTT CACAGGAAAC GATAAGTATG AAGGTTATTG TGTGGAACTG 
GCTGCAGAGA TCGCCAAACA TGTGGGATAC CACTACATAC TCAAGATAGT GGCTGATGGG 
AAATATGGAG CCAGAGATGC GGAGACCATG ATGTGGAATG GGATGGTAGG AGAGCTTGTC 
TATGGTAAAG CTGATGTAGC TGTGGCCCCG CTGACTATCA CCTTGGTCAG AGAGGAAGTC 
ATTGATTTCT CCAAGCCCTT CATGAGCCTG GGCATCTCCA TCATGATCAA AAAACCCACC 
AAATCTAAGC CTGGCGTCTT CTCCTTCCTG GACCCTCTGG CCTATGAGAT CTGGATGTGT 
ATTGTGTTTG CTTACATCGG CGTGAGCGTG GTACTTTTTC TGGTCAGCCG CTTCAGTCCC 
TATGAGTGGC ATGCAGATGA CTGTGAGGAC GGAGCAGAGG AGAACCCAAA CCAGCCGTCC 
TCATCATCCG CCCAACCGGG TCAGAACCAG CAAAACCAGA ACCAGCAGAG TCAGGAGCAA 
ACCAACGAGT TTGGCATCTT CAACAGTCTC TGGTTCTCAC TGGGAGCTTT CATGCAGCAG 
GGCTGTGACA TTTCCCCCAG GTCTCTGTCT GGCCGTATCG TGGGTGGTGT GTGGTGGTTC 
TTTACGCTAA TCATCATCTC CTCGTACACA GCCAACCTTG CTGCATTTCT CACAGTAGAG 
AGGATGGTGT CGCCCATCGA GAGTGCAGAA GATTTGGCCA AACAGACTGA GATTGCTTAC 
GGCACTCTGG ACGCAGGCTC CACCAAAGAG TTCTTCAGGA GGTCAAAGAT TGCAGTCTTT 
GAGAAGATGT GGTCTTACAT GAAGTCAGCA GATCCCTCAG TGTTTGTGAA AACCACAGAT 
GAAGGAGTTA TGCGGGTGAG GAAATCCAAA GGGAAGTATG CATACTTGCT GGAGTCCACC 
ATGAACGAGT ATATCGAGCA GCGCAAACCT TGCGACACTA TGAAGGTTGG AGGGAACCTG 
GACTCCAAAG GCTACGGTAT TGCTACCCCC AAAGGTTCCC CTTTAAGAAT CCCAGTTAAC 
CTAGCGGTGT TGAAACTCAG TGAGCAAGCC GTCTTAGACA AACTGAAAAA CAAATGGTGG 
TACGATAAGG GGGAGTGTGG AAGCAAGGAC TCCGGAAGAA AGGACAAGAC GAGTGCTCTT 
AGCCTCAGTA ACGTGGCTGG GGTTTTCTAC ATCCTGATCG GCGGTCTGGG TCTGGCCATG 
CTGGTGGCTC TGGTGGAGTT CTGCTATAAA TCCAGGACAG AGTCTCGCAG AATGAAGCAA 
TCCATCAACG ACGCCATGCA CTGCTCCACG CTGACCAGGA TGAGCGGGAA CGGCAACGGT 
GGGGAGAACG GGCGGATCGT CACACATGAC TTCCCCAAAG CTGTGCAGAC GCTGCCGTGC 
ATGAGCCACG CGGCCAGCAT GGGCCTGGGA GCCTCTGGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gria1a ( XM_005173263.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173320.1 Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19745
Clone ID Related Accession (Same CDS sequence)	XM_005173263.3
Accession Version	XM_005173263.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	glutamate receptor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173263.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGAA GCCTCACACT GCTCTGCACC AGTTTTCTGG GATTGTGCTT CGGTTCCAGT 
TTCCCAAGTA ATATCAACAT AGGAGGGTTA TTCCCAACCG AATCACACGA ATATGAAGTG 
TTTCGTTTTG CGCTCTCGCA TCACCAGGAT ATCCCTAAAC TGGTACCTCA AGTGGATATG 
GTCAAATTGG GCAATAGCTT CTCCATGACT TATGCGTTTT GTTCGCAGTT CTCCAAAGGA 
GTGTACGCAA TCATTGGCGT CTACGACAGG AAGACCGTCA ACATGTTGAT GTCGTTCTGC 
GGTGCGCTAC ACGTCTGCTT CGTGACGCCC AGCTTCCCTA TGGAAAACTC CAACCAGTTT 
GTCATCCAGC TGAGGCCTGA ACTGCAGGAC GCCCTGGTTG GTGTAATCGA GCACTACAGG 
TGGTCTAAGT TTGTCTACAT GTACAGCTCG GACTCTGGAC TGTCTGTTCT GCAGAAGGTT 
CTGGACACAG CAGCAGAGCA TAACTGGCTG GTGACCTCTG TGAACGTGGA GACAATGACA 
GAGGCGTCCT TTCTCAAAGT GTTCCAGGAT CTGGACAAAA AGAAGGAGGG CCAGATAATC 
ATCGACTGTG AGCTGGAGAG ACTCACCTCC ATTCTGAAGA AGATCATCGA GCTGGGGAAG 
AATGTGAAGA GCTACCACTA CATCCTAGCG AACCTGGGAT TTTTGGACAT TGACCTGACG 
GAGTTTAAGA AGGGTGAGGC GAATGTAACA GGCTTTCAGC TGGTGAATTA CGCAGATCCT 
AATGTCAGCA GGACAGTGCA GCAGTGGATG GAGTTTGACA ATAAAGATGC CAAAGTTCCC 
AAGCGAAGAC TGAAGTACAC TGGAGCTCTC ACATATGATG GGGTCAAAGT CATGTCCACA 
GCTTTCCAGA ACCTCCGCAG GCAGAGGATA GATATATCTC GACGCGGAAA TGCTGGAGAA 
TGTCTGGCCA ATCCACCGGC ACCCTGGGGC CAAGGCATTG ACATTCAGAG AGCGCTTCAA 
CAGGTTCGGA TTGAAGGCTT AACAGGACAC ATTCAGTTTA ACGAGAAAGG ACGAAGGACA 
AACTACTCTA TCAGTGTGAT GGAGCTCAGG CCGACGGGAC CAAAGAAAGT GGGTTACTGG 
AATGAGGATG AGAAATTTGT GAGCACGGCG GCCTACATGC CGGGAAGTAA TGAGACTTAC 
GGACTTCAGA ACCGCACATA TATTGTTACC ACTATTCTGG AATCTCCATA TGTCATGCTG 
AAGAAGAACC ATGAACATTT CACAGGAAAC GATAAGTATG AAGGTTATTG TGTGGAACTG 
GCTGCAGAGA TCGCCAAACA TGTGGGATAC CACTACATAC TCAAGATAGT GGCTGATGGG 
AAATATGGAG CCAGAGATGC GGAGACCATG ATGTGGAATG GGATGGTAGG AGAGCTTGTC 
TATGGTAAAG CTGATGTAGC TGTGGCCCCG CTGACTATCA CCTTGGTCAG AGAGGAAGTC 
ATTGATTTCT CCAAGCCCTT CATGAGCCTG GGCATCTCCA TCATGATCAA AAAACCCACC 
AAATCTAAGC CTGGCGTCTT CTCCTTCCTG GACCCTCTGG CCTATGAGAT CTGGATGTGT 
ATTGTGTTTG CTTACATCGG CGTGAGCGTG GTACTTTTTC TGGTCAGCCG CTTCAGTCCC 
TATGAGTGGC ATGCAGATGA CTGTGAGGAC GGAGCAGAGG AGAACCCAAA CCAGCCGTCC 
TCATCATCCG CCCAACCGGG TCAGAACCAG CAAAACCAGA ACCAGCAGAG TCAGGAGCAA 
ACCAACGAGT TTGGCATCTT CAACAGTCTC TGGTTCTCAC TGGGAGCTTT CATGCAGCAG 
GGCTGTGACA TTTCCCCCAG GTCTCTGTCT GGCCGTATCG TGGGTGGTGT GTGGTGGTTC 
TTTACGCTAA TCATCATCTC CTCGTACACA GCCAACCTTG CTGCATTTCT CACAGTAGAG 
AGGATGGTGT CGCCCATCGA GAGTGCAGAA GATTTGGCCA AACAGACTGA GATTGCTTAC 
GGCACTCTGG ACGCAGGCTC CACCAAAGAG TTCTTCAGGA GGTCAAAGAT TGCAGTCTTT 
GAGAAGATGT GGTCTTACAT GAAGTCAGCA GATCCCTCAG TGTTTGTGAA AACCACAGAT 
GAAGGAGTTA TGCGGGTGAG GAAATCCAAA GGGAAGTATG CATACTTGCT GGAGTCCACC 
ATGAACGAGT ATATCGAGCA GCGCAAACCT TGCGACACTA TGAAGGTTGG AGGGAACCTG 
GACTCCAAAG GCTACGGTAT TGCTACCCCC AAAGGTTCCC CTTTAAGAAA CCCAGTAAAC 
CTGGCAGTGT TAAAACTGAA TGAACAAGGC CTGTTGGACA AATTGAAAAA CAAATGGTGG 
TACGACAAGG GAGAGTGTGG CAGCGGAGGA GGTGATTCCA AGGACAAGAC GAGTGCTCTT 
AGCCTCAGTA ACGTGGCTGG GGTTTTCTAC ATCCTGATCG GCGGTCTGGG TCTGGCCATG 
CTGGTGGCTC TGGTGGAGTT CTGCTATAAA TCCAGGACAG AGTCTCGCAG AATGAAGGTG 
TCCGCTCAGG CCTTTCACTG CACCGCCTTA GACAGCAGCT ACACTGAGCT GCAGAGTTAC 
GGCCTGTACT CGCCAGACAC TGTTAAGATA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173320.1
CDS	221..2893
Translation	MQRSLTLLCTSFLGLCFGSSFPSNINIGGLFPTESHEYEVFRFALSHHQDIPKLVPQVDMVKLGNSFSMTYAFCSQFSKGVYAIIGVYDRKTVNMLMSFCGALHVCFVTPSFPMENSNQFVIQLRPELQDALVGVIEHYRWSKFVYMYSSDSGLSVLQKVLDTAAEHNWLVTSVNVETMTEASFLKVFQDLDKKKEGQIIIDCELERLTSILKKIIELGKNVKSYHYILANLGFLDIDLTEFKKGEANVTGFQLVNYADPNVSRTVQQWMEFDNKDAKVPKRRLKYTGALTYDGVKVMSTAFQNLRRQRIDISRRGNAGECLANPPAPWGQGIDIQRALQQVRIEGLTGHIQFNEKGRRTNYSISVMELRPTGPKKVGYWNEDEKFVSTAAYMPGSNETYGLQNRTYIVTTILESPYVMLKKNHEHFTGNDKYEGYCVELAAEIAKHVGYHYILKIVADGKYGARDAETMMWNGMVGELVYGKADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPTKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHADDCEDGAEENPNQPSSSSAQPGQNQQNQNQQSQEQTNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDAGSTKEFFRRSKIAVFEKMWSYMKSADPSVFVKTTDEGVMRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSPLRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALVEFCYKSRTESRRMKVSAQAFHCTALDSSYTELQSYGLYSPDTVKI

Target ORF information:

RefSeq Version	XM_005173263.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173263.3

ORF Insert Sequence:

ATGCAGCGAA GCCTCACACT GCTCTGCACC AGTTTTCTGG GATTGTGCTT CGGTTCCAGT 
TTCCCAAGTA ATATCAACAT AGGAGGGTTA TTCCCAACCG AATCACACGA ATATGAAGTG 
TTTCGTTTTG CGCTCTCGCA TCACCAGGAT ATCCCTAAAC TGGTACCTCA AGTGGATATG 
GTCAAATTGG GCAATAGCTT CTCCATGACT TATGCGTTTT GTTCGCAGTT CTCCAAAGGA 
GTGTACGCAA TCATTGGCGT CTACGACAGG AAGACCGTCA ACATGTTGAT GTCGTTCTGC 
GGTGCGCTAC ACGTCTGCTT CGTGACGCCC AGCTTCCCTA TGGAAAACTC CAACCAGTTT 
GTCATCCAGC TGAGGCCTGA ACTGCAGGAC GCCCTGGTTG GTGTAATCGA GCACTACAGG 
TGGTCTAAGT TTGTCTACAT GTACAGCTCG GACTCTGGAC TGTCTGTTCT GCAGAAGGTT 
CTGGACACAG CAGCAGAGCA TAACTGGCTG GTGACCTCTG TGAACGTGGA GACAATGACA 
GAGGCGTCCT TTCTCAAAGT GTTCCAGGAT CTGGACAAAA AGAAGGAGGG CCAGATAATC 
ATCGACTGTG AGCTGGAGAG ACTCACCTCC ATTCTGAAGA AGATCATCGA GCTGGGGAAG 
AATGTGAAGA GCTACCACTA CATCCTAGCG AACCTGGGAT TTTTGGACAT TGACCTGACG 
GAGTTTAAGA AGGGTGAGGC GAATGTAACA GGCTTTCAGC TGGTGAATTA CGCAGATCCT 
AATGTCAGCA GGACAGTGCA GCAGTGGATG GAGTTTGACA ATAAAGATGC CAAAGTTCCC 
AAGCGAAGAC TGAAGTACAC TGGAGCTCTC ACATATGATG GGGTCAAAGT CATGTCCACA 
GCTTTCCAGA ACCTCCGCAG GCAGAGGATA GATATATCTC GACGCGGAAA TGCTGGAGAA 
TGTCTGGCCA ATCCACCGGC ACCCTGGGGC CAAGGCATTG ACATTCAGAG AGCGCTTCAA 
CAGGTTCGGA TTGAAGGCTT AACAGGACAC ATTCAGTTTA ACGAGAAAGG ACGAAGGACA 
AACTACTCTA TCAGTGTGAT GGAGCTCAGG CCGACGGGAC CAAAGAAAGT GGGTTACTGG 
AATGAGGATG AGAAATTTGT GAGCACGGCG GCCTACATGC CGGGAAGTAA TGAGACTTAC 
GGACTTCAGA ACCGCACATA TATTGTTACC ACTATTCTGG AATCTCCATA TGTCATGCTG 
AAGAAGAACC ATGAACATTT CACAGGAAAC GATAAGTATG AAGGTTATTG TGTGGAACTG 
GCTGCAGAGA TCGCCAAACA TGTGGGATAC CACTACATAC TCAAGATAGT GGCTGATGGG 
AAATATGGAG CCAGAGATGC GGAGACCATG ATGTGGAATG GGATGGTAGG AGAGCTTGTC 
TATGGTAAAG CTGATGTAGC TGTGGCCCCG CTGACTATCA CCTTGGTCAG AGAGGAAGTC 
ATTGATTTCT CCAAGCCCTT CATGAGCCTG GGCATCTCCA TCATGATCAA AAAACCCACC 
AAATCTAAGC CTGGCGTCTT CTCCTTCCTG GACCCTCTGG CCTATGAGAT CTGGATGTGT 
ATTGTGTTTG CTTACATCGG CGTGAGCGTG GTACTTTTTC TGGTCAGCCG CTTCAGTCCC 
TATGAGTGGC ATGCAGATGA CTGTGAGGAC GGAGCAGAGG AGAACCCAAA CCAGCCGTCC 
TCATCATCCG CCCAACCGGG TCAGAACCAG CAAAACCAGA ACCAGCAGAG TCAGGAGCAA 
ACCAACGAGT TTGGCATCTT CAACAGTCTC TGGTTCTCAC TGGGAGCTTT CATGCAGCAG 
GGCTGTGACA TTTCCCCCAG GTCTCTGTCT GGCCGTATCG TGGGTGGTGT GTGGTGGTTC 
TTTACGCTAA TCATCATCTC CTCGTACACA GCCAACCTTG CTGCATTTCT CACAGTAGAG 
AGGATGGTGT CGCCCATCGA GAGTGCAGAA GATTTGGCCA AACAGACTGA GATTGCTTAC 
GGCACTCTGG ACGCAGGCTC CACCAAAGAG TTCTTCAGGA GGTCAAAGAT TGCAGTCTTT 
GAGAAGATGT GGTCTTACAT GAAGTCAGCA GATCCCTCAG TGTTTGTGAA AACCACAGAT 
GAAGGAGTTA TGCGGGTGAG GAAATCCAAA GGGAAGTATG CATACTTGCT GGAGTCCACC 
ATGAACGAGT ATATCGAGCA GCGCAAACCT TGCGACACTA TGAAGGTTGG AGGGAACCTG 
GACTCCAAAG GCTACGGTAT TGCTACCCCC AAAGGTTCCC CTTTAAGAAA CCCAGTAAAC 
CTGGCAGTGT TAAAACTGAA TGAACAAGGC CTGTTGGACA AATTGAAAAA CAAATGGTGG 
TACGACAAGG GAGAGTGTGG CAGCGGAGGA GGTGATTCCA AGGACAAGAC GAGTGCTCTT 
AGCCTCAGTA ACGTGGCTGG GGTTTTCTAC ATCCTGATCG GCGGTCTGGG TCTGGCCATG 
CTGGTGGCTC TGGTGGAGTT CTGCTATAAA TCCAGGACAG AGTCTCGCAG AATGAAGGTG 
TCCGCTCAGG CCTTTCACTG CACCGCCTTA GACAGCAGCT ACACTGAGCT GCAGAGTTAC 
GGCCTGTACT CGCCAGACAC TGTTAAGATA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gria1a ( XM_009291147.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289422.1 Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), transcript variant X2, mRNA.

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CloneID	ODa47286
Clone ID Related Accession (Same CDS sequence)	XM_009291147.2
Accession Version	XM_009291147.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	glutamate receptor 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291147.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGAA GCCTCACACT GCTCTGCACC AGTTTTCTGG GATTGTGCTT CGGTTCCAGT 
TTCCCAAGTA ATATCAACAT AGGAGGGTTA TTCCCAACCG AATCACACGA ATATGAAGTG 
TTTCGTTTTG CGCTCTCGCA TCACCAGGAT ATCCCTAAAC TGGTACCTCA AGTGGATATG 
GTCAAATTGG GCAATAGCTT CTCCATGACT TATGCGTTTT GTTCGCAGTT CTCCAAAGGA 
GTGTACGCAA TCATTGGCGT CTACGACAGG AAGACCGTCA ACATGTTGAT GTCGTTCTGC 
GGTGCGCTAC ACGTCTGCTT CGTGACGCCC AGCTTCCCTA TGGAAAACTC CAACCAGTTT 
GTCATCCAGC TGAGGCCTGA ACTGCAGGAC GCCCTGGTTG GTGTAATCGA GCACTACAGG 
TGGTCTAAGT TTGTCTACAT GTACAGCTCG GACTCTGGAC TGTCTGTTCT GCAGAAGGTT 
CTGGACACAG CAGCAGAGCA TAACTGGCTG GTGACCTCTG TGAACGTGGA GACAATGACA 
GAGGCGTCCT TTCTCAAAGT GTTCCAGGAT CTGGACAAAA AGAAGGAGGG CCAGATAATC 
ATCGACTGTG AGCTGGAGAG ACTCACCTCC ATTCTGAAGA AGATCATCGA GCTGGGGAAG 
AATGTGAAGA GCTACCACTA CATCCTAGCG AACCTGGGAT TTTTGGACAT TGACCTGACG 
GAGTTTAAGA AGGGTGAGGC GAATGTAACA GGCTTTCAGC TGGTGAATTA CGCAGATCCT 
AATGTCAGCA GGACAGTGCA GCAGTGGATG GAGTTTGACA ATAAAGATGC CAAAGTTCCC 
AAGCGAAGAC TGAAGTACAC TGGAGCTCTC ACATATGATG GGGTCAAAGT CATGTCCACA 
GCTTTCCAGA ACCTCCGCAG GCAGAGGATA GATATATCTC GACGCGGAAA TGCTGGAGAA 
TGTCTGGCCA ATCCACCGGC ACCCTGGGGC CAAGGCATTG ACATTCAGAG AGCGCTTCAA 
CAGGTTCGGA TTGAAGGCTT AACAGGACAC ATTCAGTTTA ACGAGAAAGG ACGAAGGACA 
AACTACTCTA TCAGTGTGAT GGAGCTCAGG CCGACGGGAC CAAAGAAAGT GGGTTACTGG 
AATGAGGATG AGAAATTTGT GAGCACGGCG GCCTACATGC CGGGAAGTAA TGAGACTTAC 
GGACTTCAGA ACCGCACATA TATTGTTACC ACTATTCTGG AATCTCCATA TGTCATGCTG 
AAGAAGAACC ATGAACATTT CACAGGAAAC GATAAGTATG AAGGTTATTG TGTGGAACTG 
GCTGCAGAGA TCGCCAAACA TGTGGGATAC CACTACATAC TCAAGATAGT GGCTGATGGG 
AAATATGGAG CCAGAGATGC GGAGACCATG ATGTGGAATG GGATGGTAGG AGAGCTTGTC 
TATGGTAAAG CTGATGTAGC TGTGGCCCCG CTGACTATCA CCTTGGTCAG AGAGGAAGTC 
ATTGATTTCT CCAAGCCCTT CATGAGCCTG GGCATCTCCA TCATGATCAA AAAACCCACC 
AAATCTAAGC CTGGCGTCTT CTCCTTCCTG GACCCTCTGG CCTATGAGAT CTGGATGTGT 
ATTGTGTTTG CTTACATCGG CGTGAGCGTG GTACTTTTTC TGGTCAGCCG CTTCAGTCCC 
TATGAGTGGC ATGCAGATGA CTGTGAGGAC GGAGCAGAGG AGAACCCAAA CCAGCCGTCC 
TCATCATCCG CCCAACCGGG TCAGAACCAG CAAAACCAGA ACCAGCAGAG TCAGGAGCAA 
ACCAACGAGT TTGGCATCTT CAACAGTCTC TGGTTCTCAC TGGGAGCTTT CATGCAGCAG 
GGCTGTGACA TTTCCCCCAG GTCTCTGTCT GGCCGTATCG TGGGTGGTGT GTGGTGGTTC 
TTTACGCTAA TCATCATCTC CTCGTACACA GCCAACCTTG CTGCATTTCT CACAGTAGAG 
AGGATGGTGT CGCCCATCGA GAGTGCAGAA GATTTGGCCA AACAGACTGA GATTGCTTAC 
GGCACTCTGG ACGCAGGCTC CACCAAAGAG TTCTTCAGGA GGTCAAAGAT TGCAGTCTTT 
GAGAAGATGT GGTCTTACAT GAAGTCAGCA GATCCCTCAG TGTTTGTGAA AACCACAGAT 
GAAGGAGTTA TGCGGGTGAG GAAATCCAAA GGGAAGTATG CATACTTGCT GGAGTCCACC 
ATGAACGAGT ATATCGAGCA GCGCAAACCT TGCGACACTA TGAAGGTTGG AGGGAACCTG 
GACTCCAAAG GCTACGGTAT TGCTACCCCC AAAGGTTCCC CTTTAAGAAA CCCAGTAAAC 
CTGGCAGTGT TAAAACTGAA TGAACAAGGC CTGTTGGACA AATTGAAAAA CAAATGGTGG 
TACGACAAGG GAGAGTGTGG CAGCGGAGGA GGTGATTCCA AGGTCAGCCC CAATGTGACA 
AACCCAGCGG GACAAGACGA GTGCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289422.1
CDS	221..2710
Translation	MQRSLTLLCTSFLGLCFGSSFPSNINIGGLFPTESHEYEVFRFALSHHQDIPKLVPQVDMVKLGNSFSMTYAFCSQFSKGVYAIIGVYDRKTVNMLMSFCGALHVCFVTPSFPMENSNQFVIQLRPELQDALVGVIEHYRWSKFVYMYSSDSGLSVLQKVLDTAAEHNWLVTSVNVETMTEASFLKVFQDLDKKKEGQIIIDCELERLTSILKKIIELGKNVKSYHYILANLGFLDIDLTEFKKGEANVTGFQLVNYADPNVSRTVQQWMEFDNKDAKVPKRRLKYTGALTYDGVKVMSTAFQNLRRQRIDISRRGNAGECLANPPAPWGQGIDIQRALQQVRIEGLTGHIQFNEKGRRTNYSISVMELRPTGPKKVGYWNEDEKFVSTAAYMPGSNETYGLQNRTYIVTTILESPYVMLKKNHEHFTGNDKYEGYCVELAAEIAKHVGYHYILKIVADGKYGARDAETMMWNGMVGELVYGKADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPTKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHADDCEDGAEENPNQPSSSSAQPGQNQQNQNQQSQEQTNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDAGSTKEFFRRSKIAVFEKMWSYMKSADPSVFVKTTDEGVMRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSPLRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKVSPNVTNPAGQDECS

Target ORF information:

RefSeq Version	XM_009291147.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, AMPA 1a (gria1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291147.2

ORF Insert Sequence:

ATGCAGCGAA GCCTCACACT GCTCTGCACC AGTTTTCTGG GATTGTGCTT CGGTTCCAGT 
TTCCCAAGTA ATATCAACAT AGGAGGGTTA TTCCCAACCG AATCACACGA ATATGAAGTG 
TTTCGTTTTG CGCTCTCGCA TCACCAGGAT ATCCCTAAAC TGGTACCTCA AGTGGATATG 
GTCAAATTGG GCAATAGCTT CTCCATGACT TATGCGTTTT GTTCGCAGTT CTCCAAAGGA 
GTGTACGCAA TCATTGGCGT CTACGACAGG AAGACCGTCA ACATGTTGAT GTCGTTCTGC 
GGTGCGCTAC ACGTCTGCTT CGTGACGCCC AGCTTCCCTA TGGAAAACTC CAACCAGTTT 
GTCATCCAGC TGAGGCCTGA ACTGCAGGAC GCCCTGGTTG GTGTAATCGA GCACTACAGG 
TGGTCTAAGT TTGTCTACAT GTACAGCTCG GACTCTGGAC TGTCTGTTCT GCAGAAGGTT 
CTGGACACAG CAGCAGAGCA TAACTGGCTG GTGACCTCTG TGAACGTGGA GACAATGACA 
GAGGCGTCCT TTCTCAAAGT GTTCCAGGAT CTGGACAAAA AGAAGGAGGG CCAGATAATC 
ATCGACTGTG AGCTGGAGAG ACTCACCTCC ATTCTGAAGA AGATCATCGA GCTGGGGAAG 
AATGTGAAGA GCTACCACTA CATCCTAGCG AACCTGGGAT TTTTGGACAT TGACCTGACG 
GAGTTTAAGA AGGGTGAGGC GAATGTAACA GGCTTTCAGC TGGTGAATTA CGCAGATCCT 
AATGTCAGCA GGACAGTGCA GCAGTGGATG GAGTTTGACA ATAAAGATGC CAAAGTTCCC 
AAGCGAAGAC TGAAGTACAC TGGAGCTCTC ACATATGATG GGGTCAAAGT CATGTCCACA 
GCTTTCCAGA ACCTCCGCAG GCAGAGGATA GATATATCTC GACGCGGAAA TGCTGGAGAA 
TGTCTGGCCA ATCCACCGGC ACCCTGGGGC CAAGGCATTG ACATTCAGAG AGCGCTTCAA 
CAGGTTCGGA TTGAAGGCTT AACAGGACAC ATTCAGTTTA ACGAGAAAGG ACGAAGGACA 
AACTACTCTA TCAGTGTGAT GGAGCTCAGG CCGACGGGAC CAAAGAAAGT GGGTTACTGG 
AATGAGGATG AGAAATTTGT GAGCACGGCG GCCTACATGC CGGGAAGTAA TGAGACTTAC 
GGACTTCAGA ACCGCACATA TATTGTTACC ACTATTCTGG AATCTCCATA TGTCATGCTG 
AAGAAGAACC ATGAACATTT CACAGGAAAC GATAAGTATG AAGGTTATTG TGTGGAACTG 
GCTGCAGAGA TCGCCAAACA TGTGGGATAC CACTACATAC TCAAGATAGT GGCTGATGGG 
AAATATGGAG CCAGAGATGC GGAGACCATG ATGTGGAATG GGATGGTAGG AGAGCTTGTC 
TATGGTAAAG CTGATGTAGC TGTGGCCCCG CTGACTATCA CCTTGGTCAG AGAGGAAGTC 
ATTGATTTCT CCAAGCCCTT CATGAGCCTG GGCATCTCCA TCATGATCAA AAAACCCACC 
AAATCTAAGC CTGGCGTCTT CTCCTTCCTG GACCCTCTGG CCTATGAGAT CTGGATGTGT 
ATTGTGTTTG CTTACATCGG CGTGAGCGTG GTACTTTTTC TGGTCAGCCG CTTCAGTCCC 
TATGAGTGGC ATGCAGATGA CTGTGAGGAC GGAGCAGAGG AGAACCCAAA CCAGCCGTCC 
TCATCATCCG CCCAACCGGG TCAGAACCAG CAAAACCAGA ACCAGCAGAG TCAGGAGCAA 
ACCAACGAGT TTGGCATCTT CAACAGTCTC TGGTTCTCAC TGGGAGCTTT CATGCAGCAG 
GGCTGTGACA TTTCCCCCAG GTCTCTGTCT GGCCGTATCG TGGGTGGTGT GTGGTGGTTC 
TTTACGCTAA TCATCATCTC CTCGTACACA GCCAACCTTG CTGCATTTCT CACAGTAGAG 
AGGATGGTGT CGCCCATCGA GAGTGCAGAA GATTTGGCCA AACAGACTGA GATTGCTTAC 
GGCACTCTGG ACGCAGGCTC CACCAAAGAG TTCTTCAGGA GGTCAAAGAT TGCAGTCTTT 
GAGAAGATGT GGTCTTACAT GAAGTCAGCA GATCCCTCAG TGTTTGTGAA AACCACAGAT 
GAAGGAGTTA TGCGGGTGAG GAAATCCAAA GGGAAGTATG CATACTTGCT GGAGTCCACC 
ATGAACGAGT ATATCGAGCA GCGCAAACCT TGCGACACTA TGAAGGTTGG AGGGAACCTG 
GACTCCAAAG GCTACGGTAT TGCTACCCCC AAAGGTTCCC CTTTAAGAAA CCCAGTAAAC 
CTGGCAGTGT TAAAACTGAA TGAACAAGGC CTGTTGGACA AATTGAAAAA CAAATGGTGG 
TACGACAAGG GAGAGTGTGG CAGCGGAGGA GGTGATTCCA AGGTCAGCCC CAATGTGACA 
AACCCAGCGG GACAAGACGA GTGCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Knockdown of Carboxypeptidase A6 in Zebrafish Larvae Reduces Response to Seizure-Inducing Drugs and Causes Changes in the Level of mRNAs Encoding Signaling Molecules.
PloS one11(4)e0152905(2016)
Lopes MW,Sapio MR,Leal RB,Fricker LD

Growth hormone (GH) increases cognition and expression of ionotropic glutamate receptors (AMPA and NMDA) in transgenic zebrafish (Danio rerio).
Behavioural brain research29436-42(2015 Nov)
Studzinski AL,Barros DM,Marins LF

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
The Journal of biological chemistry290(30)18261-8(2015 Jul)
Deng C,Chen H,Yang N,Feng Y,Hsueh AJ

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gria1a cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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