hnf1ba cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hnf1ba
Entrez Gene ID	792391
Full Name	HNF1 homeobox Ba
Synonyms	chunp6877,hnf1b,mst,tcf2,vhnf1
General protein information	Preferred Names HNF1 homeobox Ba Names hepatocyte nuclear factor 1-beta-A HNF-1-beta-A HNF-1B-2 HNF-1B-A HNF1 homeobox b TCF-2 hepatocyte nuclear factor 1-beta hepatocyte nuclear factor 1-beta-2 hob transcription factor 2, hepatic variant hepatic nuclear factor 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021481477.1	XP_021337152.1	hepatocyte nuclear factor 1-beta-A isoform X1
XM_005157431.3	XP_005157488.1	hepatocyte nuclear factor 1-beta-A isoform X1
NM_131880.3	NP_571955.3	hepatocyte nuclear factor 1-beta-A
XM_021481478.1	XP_021337153.1	hepatocyte nuclear factor 1-beta-A isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC610402	XP_005624847.1
Homo sapiens (human)	HNF1B	NP_000449.1
Pan troglodytes (chimpanzee)	HNF1B	XP_523618.2
Mus musculus (house mouse)	Hnf1b	NP_033356.2
Gallus gallus (chicken)	HNF1B	XP_004946816.1
Bos taurus (cattle)	HNF1B	NP_001179784.1
Rattus norvegicus (Norway rat)	Hnf1b	NP_037235.1
Danio rerio (zebrafish)	hnf1ba	NP_571955.3
Macaca mulatta (Rhesus monkey)	HNF1B	XP_001112223.1
Xenopus tropicalis (tropical clawed frog)	hnf1b	XP_002939679.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion.
Naylor RW, Chang HG, Qubisi S, Davidson AJ
eLife7(2018 11)

Analysis of novel caudal hindbrain genes reveals different regulatory logic for gene expression in rhombomere 4 versus 5/6 in embryonic zebrafish.
Ghosh P, Maurer JM, Sagerström CG
Neural development13(1)13(2018 06)

Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach.
Pinto RA, Almeida-Santos J, Lourenço R, Saúde L
BMC developmental biology18(1)14(2018 06)

Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development.
Selland LG, Koch S, Laraque M, Waskiewicz AJ
Mechanisms of development15028-41(2018 04)

Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish.
Naylor RW, Dodd RC, Davidson AJ
Scientific reports635647(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hnf1ba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hnf1ba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148994	XM_021481478.1 Latest version!	Danio rerio HNF1 homeobox Ba (hnf1ba), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa20321	XM_005157431.3 Latest version!	Danio rerio HNF1 homeobox Ba (hnf1ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa01216	NM_131880.3 Latest version!	Danio rerio HNF1 homeobox Ba (hnf1ba), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa148993	XM_021481477.1 Latest version!	Danio rerio HNF1 homeobox Ba (hnf1ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hnf1ba ( XM_021481478.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337153.1 Danio rerio HNF1 homeobox Ba (hnf1ba), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa148994
Clone ID Related Accession (Same CDS sequence)	XM_021481478.1
Accession Version	XM_021481478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	hepatocyte nuclear factor 1-beta-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAAAG GCTACATGCA GCAGCACAAT ATCCCTCAGC GCGAGGTGGT GGATGTGACG 
GGCCTGAATC AGTCGCACCT GTCGCAGCAC CTGAATAAAG GCACGCCTAT GAAAACGCAG 
AAACGCGCGG CGCTCTACAC CTGGTATGTG AGGAAACAGC GGGAGATCTT GCGACAATTC 
AACCAGGCCA CACAAGGCTC TGGCGCCACC ATGTTAGACA AAGGAAATCA GGATCAGGTA 
CTCCTTTTTT TCTCAGAGTT TAGTCAGTCT GGGCAAGGTA TGGTCCAGCC AGGTGATGAT 
GCTGCTATTG AGCCTGCTTG CAAGAAGCTC AGACGCAACC GTTTCAAATG GGGGCCTGCA 
TCCCAACAAA TCCTTTACCA GGCTTATGAG CGGCAGAAGA ACCCCAGCAA AGAGGAGAGA 
GAGGCATTGG TGGAGGAGTG CAACCGGGCT GAGTGCCTCC AAAGAGGAGT GTCTCCATCG 
AAAGCTCATG GACTTGGCTC CAACCTGGTC ACAGAGGTGC GAGTCTACAA CTGGTTTGCT 
AACAGAAGAA AGGAAGAAGC CTTCAGACAA AAGTTGGCTA TGGATGCCTA CAGTGGGCCA 
GCGCATAGCC TAAACTCCCT CCTTTCACAT AGTTCCCCGC ATCACCCACA AACTAGCAGC 
TCCCCACCAA GCAAGATGCA AGGTGTCCGG TACAGCCAAC AAGGTCCAGG TGAAGTCAGT 
TCTTCAACAA CGATCAATCA CCATAGCAGC AATGCCATGT CAACCAGCCA GTCAGTGTTA 
CAGCAGGTCT CTCCAGGGGC GCTGGACCCC AGTCACAGCC TCCTGTCACC TGATGCCAAG 
ATGATCTCGG TATCAGGTGG AGGTCTTCCT CCAGTAAGCA CCCTGACCAA CATCCACGCA 
TCCCACCATG TGCACCAACA GACCCCTAAC CTTATAATGC CACTCTCTGG AGTCATGGCC 
ATTGCACAAA GTGAGTGTCC ATCCATATTT GGTTTCCTTT TCAGATTATT CATACATTCT 
CCTCTTGACG TCGTAATATT CTTGCATTCA GGTTTGAACA CATCGCAAGC ACAGACGGTG 
CCTGTCATAA ACAGTGTTGC AGGCAGTCTG GCAGCACTGC AGCCAGTGCA GTTCTCTCAG 
CAGTTAAATA GCCAACACCA GCAGCTCATG CAACAGTCAT CTGGACACAT GAGCCAACAG 
TCCTTCATGG CCTCCGTCTC ACACTCACAC ATGTACCCAC ACAAGCAGGA GCCACCCCAG 
TATTCTCATT CGTCTCGGTT TCCCCCGGCC ATGGTGGTGA CAGACGCCAA CAGCCTCAGT 
ACACTGAGCT CAATGTCCTC CAGCAAACAG TGTCCACTAC AGGCCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337153.1
CDS	235..1605
Translation	MIKGYMQQHNIPQREVVDVTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREILRQFNQATQGSGATMLDKGNQDQVLLFFSEFSQSGQGMVQPGDDAAIEPACKKLRRNRFKWGPASQQILYQAYERQKNPSKEEREALVEECNRAECLQRGVSPSKAHGLGSNLVTEVRVYNWFANRRKEEAFRQKLAMDAYSGPAHSLNSLLSHSSPHHPQTSSSPPSKMQGVRYSQQGPGEVSSSTTINHHSSNAMSTSQSVLQQVSPGALDPSHSLLSPDAKMISVSGGGLPPVSTLTNIHASHHVHQQTPNLIMPLSGVMAIAQSECPSIFGFLFRLFIHSPLDVVIFLHSGLNTSQAQTVPVINSVAGSLAALQPVQFSQQLNSQHQQLMQQSSGHMSQQSFMASVSHSHMYPHKQEPPQYSHSSRFPPAMVVTDANSLSTLSSMSSSKQCPLQAW

Target ORF information:

RefSeq Version	XM_021481478.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HNF1 homeobox Ba (hnf1ba), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481478.1

ORF Insert Sequence:

ATGATCAAAG GCTACATGCA GCAGCACAAT ATCCCTCAGC GCGAGGTGGT GGATGTGACG 
GGCCTGAATC AGTCGCACCT GTCGCAGCAC CTGAATAAAG GCACGCCTAT GAAAACGCAG 
AAACGCGCGG CGCTCTACAC CTGGTATGTG AGGAAACAGC GGGAGATCTT GCGACAATTC 
AACCAGGCCA CACAAGGCTC TGGCGCCACC ATGTTAGACA AAGGAAATCA GGATCAGGTA 
CTCCTTTTTT TCTCAGAGTT TAGTCAGTCT GGGCAAGGTA TGGTCCAGCC AGGTGATGAT 
GCTGCTATTG AGCCTGCTTG CAAGAAGCTC AGACGCAACC GTTTCAAATG GGGGCCTGCA 
TCCCAACAAA TCCTTTACCA GGCTTATGAG CGGCAGAAGA ACCCCAGCAA AGAGGAGAGA 
GAGGCATTGG TGGAGGAGTG CAACCGGGCT GAGTGCCTCC AAAGAGGAGT GTCTCCATCG 
AAAGCTCATG GACTTGGCTC CAACCTGGTC ACAGAGGTGC GAGTCTACAA CTGGTTTGCT 
AACAGAAGAA AGGAAGAAGC CTTCAGACAA AAGTTGGCTA TGGATGCCTA CAGTGGGCCA 
GCGCATAGCC TAAACTCCCT CCTTTCACAT AGTTCCCCGC ATCACCCACA AACTAGCAGC 
TCCCCACCAA GCAAGATGCA AGGTGTCCGG TACAGCCAAC AAGGTCCAGG TGAAGTCAGT 
TCTTCAACAA CGATCAATCA CCATAGCAGC AATGCCATGT CAACCAGCCA GTCAGTGTTA 
CAGCAGGTCT CTCCAGGGGC GCTGGACCCC AGTCACAGCC TCCTGTCACC TGATGCCAAG 
ATGATCTCGG TATCAGGTGG AGGTCTTCCT CCAGTAAGCA CCCTGACCAA CATCCACGCA 
TCCCACCATG TGCACCAACA GACCCCTAAC CTTATAATGC CACTCTCTGG AGTCATGGCC 
ATTGCACAAA GTGAGTGTCC ATCCATATTT GGTTTCCTTT TCAGATTATT CATACATTCT 
CCTCTTGACG TCGTAATATT CTTGCATTCA GGTTTGAACA CATCGCAAGC ACAGACGGTG 
CCTGTCATAA ACAGTGTTGC AGGCAGTCTG GCAGCACTGC AGCCAGTGCA GTTCTCTCAG 
CAGTTAAATA GCCAACACCA GCAGCTCATG CAACAGTCAT CTGGACACAT GAGCCAACAG 
TCCTTCATGG CCTCCGTCTC ACACTCACAC ATGTACCCAC ACAAGCAGGA GCCACCCCAG 
TATTCTCATT CGTCTCGGTT TCCCCCGGCC ATGGTGGTGA CAGACGCCAA CAGCCTCAGT 
ACACTGAGCT CAATGTCCTC CAGCAAACAG TGTCCACTAC AGGCCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hnf1ba ( XM_005157431.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157488.1 Danio rerio HNF1 homeobox Ba (hnf1ba), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20321
Clone ID Related Accession (Same CDS sequence)	XM_005157431.3
Accession Version	XM_005157431.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	hepatocyte nuclear factor 1-beta-A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157431.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAAAG GCTACATGCA GCAGCACAAT ATCCCTCAGC GCGAGGTGGT GGATGTGACG 
GGCCTGAATC AGTCGCACCT GTCGCAGCAC CTGAATAAAG GCACGCCTAT GAAAACGCAG 
AAACGCGCGG CGCTCTACAC CTGGTATGTG AGGAAACAGC GGGAGATCTT GCGACAATTC 
AACCAGGCCA CACAAGGCTC TGGCGCCACC ATGTTAGACA AAGGAAATCA GGATCAGGTA 
CTCCTTTTTT TCTCAGAGTT TAGTCAGTCT GGGCAAGGTA TGGTCCAGCC AGGTGATGAT 
GCTGCTATTG AGCCTGCTTG CAAGAAGCTC AGACGCAACC GTTTCAAATG GGGGCCTGCA 
TCCCAACAAA TCCTTTACCA GGCTTATGAG CGGCAGAAGA ACCCCAGCAA AGAGGAGAGA 
GAGGCATTGG TGGAGGAGTG CAACCGGGCT GAGTGCCTCC AAAGAGGAGT GTCTCCATCG 
AAAGCTCATG GACTTGGCTC CAACCTGGTC ACAGAGGTGC GAGTCTACAA CTGGTTTGCT 
AACAGAAGAA AGGAAGAAGC CTTCAGACAA AAGTTGGCTA TGGATGCCTA CAGTGGGCCA 
GCGCATAGCC TAAACTCCCT CCTTTCACAT AGTTCCCCGC ATCACCCACA AACTAGCAGC 
TCCCCACCAA GCAAGATGCA AGGTGTCCGG TACAGCCAAC AAGGTCCAGG TGAAGTCAGT 
TCTTCAACAA CGATCAATCA CCATAGCAGC AATGCCATGT CAACCAGCCA GTCAGTGTTA 
CAGCAGGTCT CTCCAGGGGC GCTGGACCCC AGTCACAGCC TCCTGTCACC TGATGCCAAG 
ATGATCTCGG TATCAGGTGG AGGTCTTCCT CCAGTAAGCA CCCTGACCAA CATCCACGCA 
TCCCACCATG TGCACCAACA GACCCCTAAC CTTATAATGC CACTCTCTGG AGTCATGGCC 
ATTGCACAAA GTTTGAACAC ATCGCAAGCA CAGACGGTGC CTGTCATAAA CAGTGTTGCA 
GGCAGTCTGG CAGCACTGCA GCCAGTGCAG TTCTCTCAGC AGTTAAATAG CCAACACCAG 
CAGCTCATGC AACAGTCATC TGGACACATG AGCCAACAGT CCTTCATGGC CTCCGTCTCA 
CACTCACACA TGTACCCACA CAAGCAGGAG CCACCCCAGT ATTCTCATTC GTCTCGGTTT 
CCCCCGGCCA TGGTGGTGAC AGACGCCAAC AGCCTCAGTA CACTGAGCTC AATGTCCTCC 
AGCAAACAGT GTCCACTACA GGCCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157488.1
CDS	274..1563
Translation	MIKGYMQQHNIPQREVVDVTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREILRQFNQATQGSGATMLDKGNQDQVLLFFSEFSQSGQGMVQPGDDAAIEPACKKLRRNRFKWGPASQQILYQAYERQKNPSKEEREALVEECNRAECLQRGVSPSKAHGLGSNLVTEVRVYNWFANRRKEEAFRQKLAMDAYSGPAHSLNSLLSHSSPHHPQTSSSPPSKMQGVRYSQQGPGEVSSSTTINHHSSNAMSTSQSVLQQVSPGALDPSHSLLSPDAKMISVSGGGLPPVSTLTNIHASHHVHQQTPNLIMPLSGVMAIAQSLNTSQAQTVPVINSVAGSLAALQPVQFSQQLNSQHQQLMQQSSGHMSQQSFMASVSHSHMYPHKQEPPQYSHSSRFPPAMVVTDANSLSTLSSMSSSKQCPLQAW

Target ORF information:

RefSeq Version	XM_005157431.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HNF1 homeobox Ba (hnf1ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157431.3

ORF Insert Sequence:

ATGATCAAAG GCTACATGCA GCAGCACAAT ATCCCTCAGC GCGAGGTGGT GGATGTGACG 
GGCCTGAATC AGTCGCACCT GTCGCAGCAC CTGAATAAAG GCACGCCTAT GAAAACGCAG 
AAACGCGCGG CGCTCTACAC CTGGTATGTG AGGAAACAGC GGGAGATCTT GCGACAATTC 
AACCAGGCCA CACAAGGCTC TGGCGCCACC ATGTTAGACA AAGGAAATCA GGATCAGGTA 
CTCCTTTTTT TCTCAGAGTT TAGTCAGTCT GGGCAAGGTA TGGTCCAGCC AGGTGATGAT 
GCTGCTATTG AGCCTGCTTG CAAGAAGCTC AGACGCAACC GTTTCAAATG GGGGCCTGCA 
TCCCAACAAA TCCTTTACCA GGCTTATGAG CGGCAGAAGA ACCCCAGCAA AGAGGAGAGA 
GAGGCATTGG TGGAGGAGTG CAACCGGGCT GAGTGCCTCC AAAGAGGAGT GTCTCCATCG 
AAAGCTCATG GACTTGGCTC CAACCTGGTC ACAGAGGTGC GAGTCTACAA CTGGTTTGCT 
AACAGAAGAA AGGAAGAAGC CTTCAGACAA AAGTTGGCTA TGGATGCCTA CAGTGGGCCA 
GCGCATAGCC TAAACTCCCT CCTTTCACAT AGTTCCCCGC ATCACCCACA AACTAGCAGC 
TCCCCACCAA GCAAGATGCA AGGTGTCCGG TACAGCCAAC AAGGTCCAGG TGAAGTCAGT 
TCTTCAACAA CGATCAATCA CCATAGCAGC AATGCCATGT CAACCAGCCA GTCAGTGTTA 
CAGCAGGTCT CTCCAGGGGC GCTGGACCCC AGTCACAGCC TCCTGTCACC TGATGCCAAG 
ATGATCTCGG TATCAGGTGG AGGTCTTCCT CCAGTAAGCA CCCTGACCAA CATCCACGCA 
TCCCACCATG TGCACCAACA GACCCCTAAC CTTATAATGC CACTCTCTGG AGTCATGGCC 
ATTGCACAAA GTTTGAACAC ATCGCAAGCA CAGACGGTGC CTGTCATAAA CAGTGTTGCA 
GGCAGTCTGG CAGCACTGCA GCCAGTGCAG TTCTCTCAGC AGTTAAATAG CCAACACCAG 
CAGCTCATGC AACAGTCATC TGGACACATG AGCCAACAGT CCTTCATGGC CTCCGTCTCA 
CACTCACACA TGTACCCACA CAAGCAGGAG CCACCCCAGT ATTCTCATTC GTCTCGGTTT 
CCCCCGGCCA TGGTGGTGAC AGACGCCAAC AGCCTCAGTA CACTGAGCTC AATGTCCTCC 
AGCAAACAGT GTCCACTACA GGCCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hnf1ba ( NM_131880.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571955.3 Danio rerio HNF1 homeobox Ba (hnf1ba), mRNA.

Price & Availability↑

CloneID	ODa01216
Clone ID Related Accession (Same CDS sequence)	NM_131880.3
Accession Version	NM_131880.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Danio rerio(zebrafish)
Product	hepatocyte nuclear factor 1-beta-A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX511308.11. On or before Dec 1, 2007 this sequence version replaced XM_001331761.1, NM_131880.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY569309.1, BC129147.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505370, SAMEA3505372 [ECO:0000350] ##Evidence-Data-END##

ATGTTTGCAA ACATGGTGTC CAAGTTGACA TCTTTGCAAC AGGAGCTTCT GAGCGCCTTG 
TTGGACTCTG GAGTTACCAA AGATGTGCTT CTGCAAGCTT TGGAGGACCT GGACCCGAGC 
CCGAGCGCTT TTGGAGTTAA ACTGGACAGT CTGCAGATGT CCCCGTCCGG CTCCAAACTC 
AGCGATACGG ATTCAAAGCC GGTGTTTCAT ACGCTCACTA ATGGACACAG TAAAGGGAAG 
TTATCCGGAG ATGAAGGCTC CGAGGACGGG GATGATTATG ACACACCGCC AATTCTCAAA 
GAGCTCCAGT CTCAAAACAC CGAGGAAGCG GCCGAGCAGA GGGCAGAAAT AGAGCGAATG 
TTGGCGGAGG ACCCGTGGCG CGCGGCCCGC ATGATCAAAG GCTACATGCA GCAGCACAAT 
ATCCCTCAGC GCGAGGTGGT GGATGTGACG GGCCTGAATC AGTCGCACCT GTCGCAGCAC 
CTGAATAAAG GCACGCCTAT GAAAACGCAG AAACGCGCGG CGCTCTACAC CTGGTATGTG 
AGGAAACAGC GGGAGATCTT GCGACAATTC AACCAGGCCA CACAAGGCTC TGGCGCCACC 
ATGTTAGACA AAGGAAATCA GGATCAGGTA CTCCTTTTTT TCTCAGAGTT TAGTCAGTCT 
GGGCAAGGTA TGGTCCAGCC AGGTGATGAT GCTGCTATTG AGCCTGCTTG CAAGAAGCTC 
AGACGCAACC GTTTCAAATG GGGGCCTGCA TCCCAACAAA TCCTTTACCA GGCTTATGAG 
CGGCAGAAGA ACCCCAGCAA AGAGGAGAGA GAGGCATTGG TGGAGGAGTG CAACCGGGCT 
GAGTGCCTCC AAAGAGGAGT GTCTCCATCG AAAGCTCATG GACTTGGCTC CAACCTGGTC 
ACAGAGGTGC GAGTCTACAA CTGGTTTGCT AACAGAAGAA AGGAAGAAGC CTTCAGACAA 
AAGTTGGCTA TGGATGCCTA CAGTGGGCCA GCGCATAGCC TAAACTCCCT CCTTTCACAT 
AGTTCCCCGC ATCACCCACA AACTAGCAGC TCCCCACCAA GCAAGATGCA AGGTGTCCGG 
TACAGCCAAC AAGGTCCAGG TGAAGTCAGT TCTTCAACAA CGATCAATCA CCATAGCAGC 
AATGCCATGT CAACCAGCCA GTCAGTGTTA CAGCAGGTCT CTCCAGGGGC GCTGGACCCC 
AGTCACAGCC TCCTGTCACC TGATGCCAAG ATGATCTCGG TATCAGGTGG AGGTCTTCCT 
CCAGTAAGCA CCCTGACCAA CATCCACGCA TCCCACCATG TGCACCAACA GACCCCTAAC 
CTTATAATGC CACTCTCTGG AGTCATGGCC ATTGCACAAA GTTTGAACAC ATCGCAAGCA 
CAGACGGTGC CTGTCATAAA CAGTGTTGCA GGCAGTCTGG CAGCACTGCA GCCAGTGCAG 
TTCTCTCAGC AGTTAAATAG CCAACACCAG CAGCTCATGC AACAGTCATC TGGACACATG 
AGCCAACAGT CCTTCATGGC CTCCGTCTCA CACTCACACA TGTACCCACA CAAGCAGGAG 
CCACCCCAGT ATTCTCATTC GTCTCGGTTT CCCCCGGCCA TGGTGGTGAC AGACGCCAAC 
AGCCTCAGTA CACTGAGCTC AATGTCCTCC AGCAAACAGT GTCCACTACA GGCCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571955.3
CDS	136..1815
Translation	MFANMVSKLTSLQQELLSALLDSGVTKDVLLQALEDLDPSPSAFGVKLDSLQMSPSGSKLSDTDSKPVFHTLTNGHSKGKLSGDEGSEDGDDYDTPPILKELQSQNTEEAAEQRAEIERMLAEDPWRAARMIKGYMQQHNIPQREVVDVTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREILRQFNQATQGSGATMLDKGNQDQVLLFFSEFSQSGQGMVQPGDDAAIEPACKKLRRNRFKWGPASQQILYQAYERQKNPSKEEREALVEECNRAECLQRGVSPSKAHGLGSNLVTEVRVYNWFANRRKEEAFRQKLAMDAYSGPAHSLNSLLSHSSPHHPQTSSSPPSKMQGVRYSQQGPGEVSSSTTINHHSSNAMSTSQSVLQQVSPGALDPSHSLLSPDAKMISVSGGGLPPVSTLTNIHASHHVHQQTPNLIMPLSGVMAIAQSLNTSQAQTVPVINSVAGSLAALQPVQFSQQLNSQHQQLMQQSSGHMSQQSFMASVSHSHMYPHKQEPPQYSHSSRFPPAMVVTDANSLSTLSSMSSSKQCPLQAW

Target ORF information:

RefSeq Version	NM_131880.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HNF1 homeobox Ba (hnf1ba), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131880.3

ORF Insert Sequence:

ATGTTTGCAA ACATGGTGTC CAAGTTGACA TCTTTGCAAC AGGAGCTTCT GAGCGCCTTG 
TTGGACTCTG GAGTTACCAA AGATGTGCTT CTGCAAGCTT TGGAGGACCT GGACCCGAGC 
CCGAGCGCTT TTGGAGTTAA ACTGGACAGT CTGCAGATGT CCCCGTCCGG CTCCAAACTC 
AGCGATACGG ATTCAAAGCC GGTGTTTCAT ACGCTCACTA ATGGACACAG TAAAGGGAAG 
TTATCCGGAG ATGAAGGCTC CGAGGACGGG GATGATTATG ACACACCGCC AATTCTCAAA 
GAGCTCCAGT CTCAAAACAC CGAGGAAGCG GCCGAGCAGA GGGCAGAAAT AGAGCGAATG 
TTGGCGGAGG ACCCGTGGCG CGCGGCCCGC ATGATCAAAG GCTACATGCA GCAGCACAAT 
ATCCCTCAGC GCGAGGTGGT GGATGTGACG GGCCTGAATC AGTCGCACCT GTCGCAGCAC 
CTGAATAAAG GCACGCCTAT GAAAACGCAG AAACGCGCGG CGCTCTACAC CTGGTATGTG 
AGGAAACAGC GGGAGATCTT GCGACAATTC AACCAGGCCA CACAAGGCTC TGGCGCCACC 
ATGTTAGACA AAGGAAATCA GGATCAGGTA CTCCTTTTTT TCTCAGAGTT TAGTCAGTCT 
GGGCAAGGTA TGGTCCAGCC AGGTGATGAT GCTGCTATTG AGCCTGCTTG CAAGAAGCTC 
AGACGCAACC GTTTCAAATG GGGGCCTGCA TCCCAACAAA TCCTTTACCA GGCTTATGAG 
CGGCAGAAGA ACCCCAGCAA AGAGGAGAGA GAGGCATTGG TGGAGGAGTG CAACCGGGCT 
GAGTGCCTCC AAAGAGGAGT GTCTCCATCG AAAGCTCATG GACTTGGCTC CAACCTGGTC 
ACAGAGGTGC GAGTCTACAA CTGGTTTGCT AACAGAAGAA AGGAAGAAGC CTTCAGACAA 
AAGTTGGCTA TGGATGCCTA CAGTGGGCCA GCGCATAGCC TAAACTCCCT CCTTTCACAT 
AGTTCCCCGC ATCACCCACA AACTAGCAGC TCCCCACCAA GCAAGATGCA AGGTGTCCGG 
TACAGCCAAC AAGGTCCAGG TGAAGTCAGT TCTTCAACAA CGATCAATCA CCATAGCAGC 
AATGCCATGT CAACCAGCCA GTCAGTGTTA CAGCAGGTCT CTCCAGGGGC GCTGGACCCC 
AGTCACAGCC TCCTGTCACC TGATGCCAAG ATGATCTCGG TATCAGGTGG AGGTCTTCCT 
CCAGTAAGCA CCCTGACCAA CATCCACGCA TCCCACCATG TGCACCAACA GACCCCTAAC 
CTTATAATGC CACTCTCTGG AGTCATGGCC ATTGCACAAA GTTTGAACAC ATCGCAAGCA 
CAGACGGTGC CTGTCATAAA CAGTGTTGCA GGCAGTCTGG CAGCACTGCA GCCAGTGCAG 
TTCTCTCAGC AGTTAAATAG CCAACACCAG CAGCTCATGC AACAGTCATC TGGACACATG 
AGCCAACAGT CCTTCATGGC CTCCGTCTCA CACTCACACA TGTACCCACA CAAGCAGGAG 
CCACCCCAGT ATTCTCATTC GTCTCGGTTT CCCCCGGCCA TGGTGGTGAC AGACGCCAAC 
AGCCTCAGTA CACTGAGCTC AATGTCCTCC AGCAAACAGT GTCCACTACA GGCCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hnf1ba ( XM_021481477.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337152.1 Danio rerio HNF1 homeobox Ba (hnf1ba), transcript variant X1, mRNA.

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CloneID	ODa148993
Clone ID Related Accession (Same CDS sequence)	XM_021481477.1
Accession Version	XM_021481477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	hepatocyte nuclear factor 1-beta-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCAA ACATGGTGTC CAAGTTGACA TCTTTGCAAC AGGAGCTTCT GAGCGCCTTG 
TTGGACTCTG GAGTTACCAA AGATGTGCTT CTGCAAGCTT TGGAGGACCT GGACCCGAGC 
CCGAGCGCTT TTGGAGTTAA ACTGGACAGT CTGCAGATGT CCCCGTCCGG CTCCAAACTC 
AGCGATACGG ATTCAAAGCC GGTGTTTCAT ACGCTCACTA ATGGACACAG TAAAGGGAAG 
TTATCCGGAG ATGAAGGCTC CGAGGACGGG GATGATTATG ACACACCGCC AATTCTCAAA 
GAGCTCCAGT CTCAAAACAC CGAGGAAGCG GCCGAGCAGA GGGCAGAAAT AGAGCGAATG 
TTGGCGGAGG ACCCGTGGCG CGCGGCCCGC ATGATCAAAG GCTACATGCA GCAGCACAAT 
ATCCCTCAGC GCGAGGTGGT GGATGTGACG GGCCTGAATC AGTCGCACCT GTCGCAGCAC 
CTGAATAAAG GCACGCCTAT GAAAACGCAG AAACGCGCGG CGCTCTACAC CTGGTATGTG 
AGGAAACAGC GGGAGATCTT GCGACAATTC AACCAGGCCA CACAAGGCTC TGGCGCCACC 
ATGTTAGACA AAGGAAATCA GGATCAGGTA CTCCTTTTTT TCTCAGAGTT TAGTCAGTCT 
GGGCAAGGTA TGGTCCAGCC AGGTGATGAT GCTGCTATTG AGCCTGCTTG CAAGAAGCTC 
AGACGCAACC GTTTCAAATG GGGGCCTGCA TCCCAACAAA TCCTTTACCA GGCTTATGAG 
CGGCAGAAGA ACCCCAGCAA AGAGGAGAGA GAGGCATTGG TGGAGGAGTG CAACCGGGCT 
GAGTGCCTCC AAAGAGGAGT GTCTCCATCG AAAGCTCATG GACTTGGCTC CAACCTGGTC 
ACAGAGGTGC GAGTCTACAA CTGGTTTGCT AACAGAAGAA AGGAAGAAGC CTTCAGACAA 
AAGTTGGCTA TGGATGCCTA CAGTGGGCCA GCGCATAGCC TAAACTCCCT CCTTTCACAT 
AGTTCCCCGC ATCACCCACA AACTAGCAGC TCCCCACCAA GCAAGATGCA AGGTGTCCGG 
TACAGCCAAC AAGGTCCAGG TGAAGTCAGT TCTTCAACAA CGATCAATCA CCATAGCAGC 
AATGCCATGT CAACCAGCCA GTCAGTGTTA CAGCAGGTCT CTCCAGGGGC GCTGGACCCC 
AGTCACAGCC TCCTGTCACC TGATGCCAAG ATGATCTCGG TATCAGGTGG AGGTCTTCCT 
CCAGTAAGCA CCCTGACCAA CATCCACGCA TCCCACCATG TGCACCAACA GACCCCTAAC 
CTTATAATGC CACTCTCTGG AGTCATGGCC ATTGCACAAA GTGAGTGTCC ATCCATATTT 
GGTTTCCTTT TCAGATTATT CATACATTCT CCTCTTGACG TCGTAATATT CTTGCATTCA 
GGTTTGAACA CATCGCAAGC ACAGACGGTG CCTGTCATAA ACAGTGTTGC AGGCAGTCTG 
GCAGCACTGC AGCCAGTGCA GTTCTCTCAG CAGTTAAATA GCCAACACCA GCAGCTCATG 
CAACAGTCAT CTGGACACAT GAGCCAACAG TCCTTCATGG CCTCCGTCTC ACACTCACAC 
ATGTACCCAC ACAAGCAGGA GCCACCCCAG TATTCTCATT CGTCTCGGTT TCCCCCGGCC 
ATGGTGGTGA CAGACGCCAA CAGCCTCAGT ACACTGAGCT CAATGTCCTC CAGCAAACAG 
TGTCCACTAC AGGCCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337152.1
CDS	141..1901
Translation	MFANMVSKLTSLQQELLSALLDSGVTKDVLLQALEDLDPSPSAFGVKLDSLQMSPSGSKLSDTDSKPVFHTLTNGHSKGKLSGDEGSEDGDDYDTPPILKELQSQNTEEAAEQRAEIERMLAEDPWRAARMIKGYMQQHNIPQREVVDVTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREILRQFNQATQGSGATMLDKGNQDQVLLFFSEFSQSGQGMVQPGDDAAIEPACKKLRRNRFKWGPASQQILYQAYERQKNPSKEEREALVEECNRAECLQRGVSPSKAHGLGSNLVTEVRVYNWFANRRKEEAFRQKLAMDAYSGPAHSLNSLLSHSSPHHPQTSSSPPSKMQGVRYSQQGPGEVSSSTTINHHSSNAMSTSQSVLQQVSPGALDPSHSLLSPDAKMISVSGGGLPPVSTLTNIHASHHVHQQTPNLIMPLSGVMAIAQSECPSIFGFLFRLFIHSPLDVVIFLHSGLNTSQAQTVPVINSVAGSLAALQPVQFSQQLNSQHQQLMQQSSGHMSQQSFMASVSHSHMYPHKQEPPQYSHSSRFPPAMVVTDANSLSTLSSMSSSKQCPLQAW

Target ORF information:

RefSeq Version	XM_021481477.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HNF1 homeobox Ba (hnf1ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481477.1

ORF Insert Sequence:

ATGTTTGCAA ACATGGTGTC CAAGTTGACA TCTTTGCAAC AGGAGCTTCT GAGCGCCTTG 
TTGGACTCTG GAGTTACCAA AGATGTGCTT CTGCAAGCTT TGGAGGACCT GGACCCGAGC 
CCGAGCGCTT TTGGAGTTAA ACTGGACAGT CTGCAGATGT CCCCGTCCGG CTCCAAACTC 
AGCGATACGG ATTCAAAGCC GGTGTTTCAT ACGCTCACTA ATGGACACAG TAAAGGGAAG 
TTATCCGGAG ATGAAGGCTC CGAGGACGGG GATGATTATG ACACACCGCC AATTCTCAAA 
GAGCTCCAGT CTCAAAACAC CGAGGAAGCG GCCGAGCAGA GGGCAGAAAT AGAGCGAATG 
TTGGCGGAGG ACCCGTGGCG CGCGGCCCGC ATGATCAAAG GCTACATGCA GCAGCACAAT 
ATCCCTCAGC GCGAGGTGGT GGATGTGACG GGCCTGAATC AGTCGCACCT GTCGCAGCAC 
CTGAATAAAG GCACGCCTAT GAAAACGCAG AAACGCGCGG CGCTCTACAC CTGGTATGTG 
AGGAAACAGC GGGAGATCTT GCGACAATTC AACCAGGCCA CACAAGGCTC TGGCGCCACC 
ATGTTAGACA AAGGAAATCA GGATCAGGTA CTCCTTTTTT TCTCAGAGTT TAGTCAGTCT 
GGGCAAGGTA TGGTCCAGCC AGGTGATGAT GCTGCTATTG AGCCTGCTTG CAAGAAGCTC 
AGACGCAACC GTTTCAAATG GGGGCCTGCA TCCCAACAAA TCCTTTACCA GGCTTATGAG 
CGGCAGAAGA ACCCCAGCAA AGAGGAGAGA GAGGCATTGG TGGAGGAGTG CAACCGGGCT 
GAGTGCCTCC AAAGAGGAGT GTCTCCATCG AAAGCTCATG GACTTGGCTC CAACCTGGTC 
ACAGAGGTGC GAGTCTACAA CTGGTTTGCT AACAGAAGAA AGGAAGAAGC CTTCAGACAA 
AAGTTGGCTA TGGATGCCTA CAGTGGGCCA GCGCATAGCC TAAACTCCCT CCTTTCACAT 
AGTTCCCCGC ATCACCCACA AACTAGCAGC TCCCCACCAA GCAAGATGCA AGGTGTCCGG 
TACAGCCAAC AAGGTCCAGG TGAAGTCAGT TCTTCAACAA CGATCAATCA CCATAGCAGC 
AATGCCATGT CAACCAGCCA GTCAGTGTTA CAGCAGGTCT CTCCAGGGGC GCTGGACCCC 
AGTCACAGCC TCCTGTCACC TGATGCCAAG ATGATCTCGG TATCAGGTGG AGGTCTTCCT 
CCAGTAAGCA CCCTGACCAA CATCCACGCA TCCCACCATG TGCACCAACA GACCCCTAAC 
CTTATAATGC CACTCTCTGG AGTCATGGCC ATTGCACAAA GTGAGTGTCC ATCCATATTT 
GGTTTCCTTT TCAGATTATT CATACATTCT CCTCTTGACG TCGTAATATT CTTGCATTCA 
GGTTTGAACA CATCGCAAGC ACAGACGGTG CCTGTCATAA ACAGTGTTGC AGGCAGTCTG 
GCAGCACTGC AGCCAGTGCA GTTCTCTCAG CAGTTAAATA GCCAACACCA GCAGCTCATG 
CAACAGTCAT CTGGACACAT GAGCCAACAG TCCTTCATGG CCTCCGTCTC ACACTCACAC 
ATGTACCCAC ACAAGCAGGA GCCACCCCAG TATTCTCATT CGTCTCGGTT TCCCCCGGCC 
ATGGTGGTGA CAGACGCCAA CAGCCTCAGT ACACTGAGCT CAATGTCCTC CAGCAAACAG 
TGTCCACTAC AGGCCTGGTG A