notch3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	notch3
Entrez Gene ID	58066
Full Name	notch 3
Synonyms	fa14b08,notch5,wu:fa14b08
General protein information	Preferred Names notch 3 Names neurogenic locus notch homolog protein 3 notch homolog 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131549.2	NP_571624.2	neurogenic locus notch homolog protein 3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	notch3	NP_571624.2
Caenorhabditis elegans (roundworm)	lin-12	NP_499007.1
Caenorhabditis elegans (roundworm)	glp-1	NP_499014.1

Gene Ontology
Bibliography

Related articles in PubMed

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Xue Y, Liu D, Cui G, Ding Y, Ai D, Gao S, Zhang Y, Suo S, Wang X, Lv P, Zhou C, Li Y, Chen X, Peng G, Jing N, Han JJ, Liu F
Cell reports27(5)1567-1578.e5(2019 04)

The Progress of CRISPR/Cas9-Mediated Gene Editing in Generating Mouse/Zebrafish Models of Human Skeletal Diseases.
Wu N, Liu B, Du H, Zhao S, Li Y, Cheng X, Wang S, Lin J, Zhou J, , Qiu G, Wu Z, Zhang J
Computational and structural biotechnology journal17954-962(2019)

Rescue of hematopoietic stem/progenitor cells formation in plcg1 zebrafish mutant.
Ferri-Lagneau KF, Haider J, Sang S, Leung T
Scientific reports9(1)244(2019 Jan)

Notch Inhibition Promotes Differentiation of Liver Progenitor Cells into Hepatocytes via
Russell JO, Ko S, Monga SP, Shin D
Stem cells international20198451282(2019)

A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion.
Naylor RW, Chang HG, Qubisi S, Davidson AJ
eLife7(2018 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following notch3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the notch3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03210	NM_131549.2 Latest version!	Danio rerio notch receptor 3 (notch3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

notch3 ( NM_131549.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571624.2 Danio rerio notch receptor 3 (notch3), mRNA.

Price & Availability↑

CloneID	ODa03210
Clone ID Related Accession (Same CDS sequence)	NM_131549.2
Accession Version	NM_131549.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576252800000
Organism	Danio rerio(zebrafish)
Product	neurogenic locus notch homolog protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF152001.1. On or before Jul 6, 2005 this sequence version replaced XM_702556.1, XM_679661.1, NM_131549.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF152001.1, GDQH01001522.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGGAATT ACAGCCTTTG GATATTTTTG TCAATTTTAT ATCTGGTGCA CAACAGCGAA 
GGATTGCTCT GCGTGGATAC ACACAGGCCG TGCGAAAATG GAGGAATGTG CATTGATTCA 
AGATGCTTAT GCCGGCCTGG TTATATTGGT TCGCTCTGTC AGCATCTGGA TCCCTGTCAC 
CGTTCGCCTT GTCTAAATGC AGCAGCTTGC AAGAGCCAAG TGGCGAATTC AATCCCTCAG 
TACACCTGTG TGTGTCAGCG AGGCTTTAGA GGTCAGGATT GTTCTCTCGT TGATGCGTGT 
GCCACAAGTC CTTGTGCCAA TGGTGCTCGT TGTACCAATT GGAATAATCA CTACAACTGC 
TCATGCCCAC CTGGGTATCA GGGAAAGAAC TGTCGCAATG ATATTGATGA GTGCCGCAAA 
CCCGGAAAGT GTCTTAATGG TGGCATCTGC ATGAATACAC ATGGCTCATT TCGCTGTGAG 
TGCCTTACAG GGTACAGCGG GCGCACATGC GAGGTGCCCA CCCAGCCCTG TGCCCCATCA 
CAATGTCTCA ACGGCGGCAC CTGCCATCAG ACCGGTGACC ACACCTACGA ATGTGCTTGT 
TTGCCAGGCT TCAGGGGACA CAATTGTGAG GACAATGTTG ATGACTGCCC TGGACACAAA 
TGCATGAATA ATGGGATCTG CGTCGATGGT GTCAACACAT ATAATTGCCA GTGCCCTCCT 
GAGTGGACAG GCCAGTACTG TGCTGAGGAT GTCAACGAGT GTCTCATGCA GCCCAATGCT 
TGTCACAATG GTGGTACATG CTTTAACACT ATTGGAGGGC ACACCTGTGT ATGCGTTAAT 
GGCTGGACTG GGGATGACTG CAGTGAAAAC ATTGACGACT GTGCCACAGC TGTCTGCTTC 
AACGGGGCCA CATGTCATGA CCGTGTGGCC TCCTTTTTCT GCGAGTGCCC TGTGGGCAAA 
ACTGGTTTAC TCTGTCATCT GGATGATGCT TGTGTCAGTA ACCCCTGTAA TGAGGGTGCG 
GTGTGTGACA CTAACCCACT GAATGGCCGG GCAATCTGCA CCTGCCCAGC TGGCTTTGTT 
GGTGGTGCCT GCAACCAAGA CATGGATGAA TGTTCCATTG GTGCCAACCC CTGTGAGCAT 
TTTGGGAAGT GTGTAAACAC TGAAGGCTCG TTCCAGTGCC AGTGTGGACG GGGCTACACT 
GGCCCTCGCT GCGAGATTGA TATCAACGAG TGTCTGTCCA TGCCTTGCCA AAACGATGCC 
ACCTGCCTGG ACCGCATTGG CGAGTTCACA TGCATCTGTA TGCCTGGCTA TCAGGGGAAG 
TACTGTGAGG TGGACATTGA TGAATGCGAG AGCAACCCCT GCGTAAATGA TGGCATCTGT 
CGAGATATGG TCAACGGCTT CACCTGCACC TGTCAGCCAG GGTTTACTGG CACCATGTGT 
CAAATCGACA TTGATGAGTG CGCCAGCACT CCTTGCCAGA ATGGAGCCAA GTGCATTGAC 
CGACCTAATG GATATGAATG CCGCTGTGCT GAAGGTTTTG AAGGAAGACT GTGTGAGAGC 
AACATTGATA ATTGTAAGCC TGACCCATGC CACCATGGTA CTTGCGTAGA TGGCATTGCC 
AGTTATACTT GCAACTGTGA GCCTGGCTAC ACTGGCTATC GCTGCGAAAA CCAGCTCAAT 
GAGTGCCATA GCAACCCCTG CCAGAATGGT GGCAAATGTG TTGACCTTGT CAACAAATAC 
ATCTGCCAGT GTCAACATGG CACTTCAGGT ACCAACTGTG AAATCAACTT TGACGACTGT 
GCTAGCAACC CTTGTGACTA CGGAATTTGC AAAGATGGCA TAAATCGCTA TGAGTGTGTC 
TGCAAACCTG GCTTTACCGG ACCTCAATGT AAGGATGAGA TAGATGAGTG CCAGTCGAAT 
CCCTGTCGCA ATGGAGGGAC CTGTGTAGAT GATGAGAATG GCTTCCACTG TCAGTGCCCT 
GAAGGGTTCC ATGATCCCTA CTGCTATTCA CAAGTGGATG AGTGTGCCAG CAATCCATGT 
TTACATGGGA CCTGCAGGGA TGACCCTAAT GGCTATCGGT GTGACTGTGA GCCTGGATGG 
GTGGGTAAGA ACTGTGACCT AGACCGAAAC GACTGTTTGC CCAGCCCCTG CCAGAATGCA 
GGCACCTGCT TTGACCAGCT CAATGGTTTC ACCTGCAAGT GTCGCCAAGG TTTTAGAGGT 
AACTTATGTC AGGTGAACAT CAATGAGTGT GCCTCCAGCC CCTGTCTAAA TCAGGGCACC 
TGTGTGGATG GAGTTGCTAG TTTCACCTGT CTCTGTGAAC CACCTTATAG TGGACCCACT 
TGTGCAGAAC TGCTCACACC TTGCTCTCCC AACCCCTGTG CCAACCATGC TTCCTGCGTG 
CACACACCTG ACTACCTAGG CTACCAGTGT AACTGCCAAC CAGGCTGGCA AGGCCATTTG 
TGCAACAATG ACATCAATGA ATGCACCTCT AACCCGTGTA AGAACCGTGG GACTTGCACA 
AATACCCTTG GAGGCTATGT GTGCTCCTGC CGTGCTGGCT ACACTGGACC CAATTGTGAA 
ACTGATATCA ATGACTGCTC GCCTAACCCC TGTCTGAATG GTGGATCCTG TACTGATGGA 
GTAAACTCAT TCCGCTGCAG TTGTCTGCCA GGCTTTACGG GGGCACGCTG TGCGACTGAG 
CTGAATGAAT GTCAGAGTGT ACCCTGCAAG AATGGTGGTA CCTGCACTGA TTATGTCAAC 
AGCTATACTT GTACCTGTAA ACCAGGCTTC ACAGGCCTTC TGTGTGAGAC CAACGTACCA 
GACTGCACTG AGAGCTCCTG TTTTAATGGG GGAACCTGCA CTGATGGGAT CAATGGTTTC 
AAATGCACCT GCCGCACAGG ATTCACTGGA GATTACTGCC AGTATGAAGT TAACGAGTGT 
GACTCTCAGC CATGCCTCAA TGGAGGAATT TGTCAAGATG CCATGGAGTC ATATCGCTGC 
TCATGTCCAA AAGGCTACAC TGGCCCACGT TGCCAGTATC CAGTTGATTG GTGCAGACCT 
TCTAACCCAT GCAAGAATGG TGGTCGCTGC AGACAAAAAG ATGCCTCCTT CACATGTGAC 
TGTTTGGGTG GCTGGTCAGG TCGTTATTGT GATATCCCAG GAGTGTCCTG TGAAGTCGCA 
GCCAGACAAA GAGGCCGCCA GACAGATGAG CTTTGCCTTC ACGGTGGACA CTGTGTCAAC 
ACTGGCAACA CGCACTACTG CAAATGTCCG GCAGACTACA CAGGCAGTTA CTGCGAGTCT 
CAGTTTGACC ACTGTGAGGA AAAACCTTGC CTCAATGGCG CCACCTGCAG AAGCTACATG 
GGAGGATTTA CTTGTGATTG TATGCCTGGT TATGAGGGGA ACAACTGTGA GAGAGAGGTC 
AATGAGTGCC AGTCTCACCC ATGTCAGAAT GGAGGAACCT GCATTGACTT AGTGGGACAT 
TACATCTGCT CCTGCCCTCC AGGAACACTG GGTGTACTCT GTGAGATAAA TGAGGATGAT 
TGTGCAACTC CCTCATGGCC TCGGGGAATG CCCAAATGTC AAAATAATGG CACATGTGTG 
GACAGAGTTG GAGGGTACAG ATGTAACTGT CCTCCAGGCT TCACTGGAGA ACGTTGCGAG 
GGAGACATCA ACGAATGTCT TTCCAACCCC TGCAACCCCT CAAACAGCTT GGACTGCATA 
CAGTTGCCCA ATGATTACCA ATGTGTGTGC AAACCTGGCT TTACAGGACG AGGGTGTCAA 
AGTAGATTCA GCGTGTGCGA GTCTCAGCCC TGTAAAAATG GAGGAGTATG TTCCTTGTCT 
GGCAGCTCCC CACCAGGATA CTCTTGCACT TGTCAGCTTG GGTACGCAGG TTCTAACTGT 
GAGAGGAGCA TGAACTGCAA AGAGCTGCCT TGCTACAATG GAGGCAGTTG CACTCTCACC 
ACACGAGGGG CACGTTGTAC TTGCATTCAG GGCTTTGGTG GGCCATTGTG TCAGCACCGC 
AGCAATGACG GCTGCTCCTC CAAGCCATGC CACAATGGAG GCTTATGTAC AGAGGAAACC 
AGCTATCCAT TTTTTCACTG CCAGTGCACC AATGGCTGGA AAGGCAAACG ATGTGAACAG 
AAAACTGGGT CATCGGCTCC ACTTCCTTCT CCTTGCCCTA TTGCTGACTG CTTCAGCAAG 
GCCAATGACG GTGTGTGTGA CAAAGAATGC AACTCCTTAG ACTGCCGCTG GGACGGAGGT 
GACTGTTCTC TTGCTGTAAA CCCCTGGGCA CGCTGTGCAG ACCCTCGATG CTGGCGGCTC 
TTCAATAACA GCCAATGTGA CGAGTTTTGC AATAACGCAG AGTGCCTGTT CGATAATTTC 
GACTGCATAA ACAAAGAGAA AGTCTGCAAC CCCATCTATG AAGCATACTG CACTGACCAT 
TATGCAGATG GACTCTGTGA TCAAGGATGC AATACTGAAG AATGTGGCTG GGATGGACTG 
GATTGTGCAA GGAAGATTCC AGAAGACCTT GCTGAAGATA TGCTTGTTAT TGTAGTCCTA 
CTGCCCCCAG AGGAGCTTCT AAGGACACAA ACAGCTTTCC TGCAGAAACT AAGTGCCATC 
CTACGCACCA CGTTGCGCTT CCGTCTCGAT CGAAATGGAG ATTACATGAT CCGACCTTAC 
ACAGGCCGTG AGACGCGCAT TAAACGGGAG CTTAATCCTC AGGAGGTCAT TGGGTCCATT 
GTATATTTGG AAATAGACAA CAGGCTGTGC TCTCAGGGCT CAGATGACTG CTTCCGCAAT 
GCTGACAGTG CTGCTGAATA CCTTGGTGCT CTGTCTGCTC GGGAGATGTT GCGCTTCCCA 
TACCCCATTA AAGAAGTGAC AAGTGAAAAA AGAGAGCCCT CGATTACTGA GATACCCGAA 
TGGGCTAGGC TACTGCTAGT AGGTGTAGCT TCTCTCTTCT TGTTAGTAAT CTTGATGGTT 
GGCATGTTGA TTGCCCGCCG CAAGCGTGAA CACAGCACAC TCTGGTTCCC TGAGGGCTTC 
TTCCTCAAAA AGGAAACAAG CAGTAACAAG AACCGCAGAG AACCTGTGGG CCAAGATTCA 
CTGGGCATGA AACACATGCC AAAGACAGTG GAAGAGTCTC TTCTGGCTGA TCACAGTGAC 
CAGTGGATAG ATACAGACTG CCCAGAGGCT AAGCGACTTA AGGTGGAAGA ACCAAGCATT 
CTGTCAGATG GTGAGGATGC AGTTGACAGC AGACAGTGGA CACAGCATCA CTTGGCAGCA 
GCAGATATCC GCATGCCACC TTCCATGGCA CTTACACCAC CACAAGGAGA GTTTGACAGT 
GACTGCATGG ATGTTAATGT CAGAGGCCCT GATGGCTTCA CACCTCTGAT GCTGGCATCT 
TTCTGTGGAG GAGGGCTTGA GCCAGAGGTG ACCGAAGATG ATGACTCAGA TGAATCCTCA 
GCCAACATCA TATCTGACCT CATTTACCAG GGAGCATCAC TTGCAGCTCA AACTGACCGC 
ACTGGAGAGA CTGCTCTGCA CCTGGCTGCA CGCTATGCCC GGGCAGATGC AGCTAAAAGA 
CTCCTGGATG CTGGGGCTGA TGCAAATGCA CAGGATAACA CAGGTCGCTC ACCTTTGCAT 
GCAGCTGTGG CAGCTGATGC ACAGGGAGTT TTCCAGATTC TGATCAGGAA CCGTGCCACA 
GACTTGGACG CGCGCATGTA TGATGGCTCC ACTGCTCTGA TACTTGCAGC CCGTCTGGCA 
GTAGAGGGCA TGGTGGAAGA GTTGATCACC TGCCATGCTG ATGTTAATGC TGTTGATGAA 
ATCGGAAAGT CAGCATTGCA CTGGGCAGCA GCAGTCAATA ACGTTGAAGC TACTATTGCC 
TTGTTGAAGA ATGGTGCCAA TAAAGATATG CAAGACCTCA AGGAGGAGAC TCCGCTGTTC 
TTGGCTGCCA GGGAAGGCAG CTGTGAGGCA GTAAAGGTGT TGTTGGCTCA CTTTGCCAAT 
AGGGAAATAA CAGATCACAT GGACAGACTC CCTCGAGATA TTGCCCAGGA GCGCATGCAT 
CATGACATTG TGCAGCTACT GGATGAATAT AACACTGTGC GAAGTCCACA GGGTCATGCA 
GGGGCAGGTC ACCACCTCTC TGGTAGCCAC ACACTCTCAC CGCTCATGTG CCCTCCCAGC 
AACTTCCTCC AAGGGCTGAA AAGTACCCCT CAGGGTAAGA AGAACCGTCG CCCGGGAGCT 
AAAGGCATTG GTGGACAGCA CGCTTCCGGC CTGAAAGATT CAGCCAAAGG ACGCAACAAA 
CGGCTGACTC TAGACATGCA GAGTGCTTTG CTGGAGAGCT CTGTCACGCT CTCCCCTGTT 
GACTCACTGG ATTCACCACG AGGAGGCGCC AGTAACGCTG GCTATGTCAC CAATCCAACC 
TCACCAGCTG CCATGCCCTC TCCCGGCCTT TTCCACTCCT CTATGTCTGT CCCTAATACC 
CCCATGGTGC ACAGCAGTAT CCTGGACAGC ACCAGCCCTT TTTCTGTCTC CCTGGCACAG 
TTGAGTGACC TGGGAGATGG TGGGCTCTCA ATGCAGGGGC GTGTGGGCAT GCAAGGAGGG 
GGTGTCAACC CAGGCCCCCA TAACTACGTG ATGAACGCCG GTCAAATGAG CCTTAGTATG 
GGCATGGTAA GTCCAGTAAG CGTGCCGTTT GACTGGCATA ACCGCATGCC TCCATCTTCT 
CAGTGCAGTC AGCCAATGAT CAGCATGGTG CATCCAGTAA GTAGTCAAGC AGGCATGCTC 
CCACAAACAC CAACCCTGCA AAGCAGCATG CTATTGCACC AGCAGGTGTT CCGTAATGCC 
CAGCAGCCCA TCCTGCAGCC CACACCTGTC TCCAGCACAC CGTCTATTAG TCCAGTCAAA 
CTGCCTCCCA TTGCTGAGCA GCAGCCGCAG CAACTTCACA GCCACACCCT CACCAACCCC 
AACCTCTCCC GCATGGCCAC CTCCACTCCC CCAACTCCAC AGCAGGCACA GCCTCCTCCA 
GCTTTCTACC AACCACAACA GCCACAAGCT CAGCCGCCGC AGGCTCCGAC AGCCCCTCAG 
GCAACGCAAG CACAAGCTAT TCCATCTCAG GCACCCCCAG CACAGGTGAG CGGCAGCACC 
GCAGGCCCAG AGGATTACCC CACACCACCT TCCCAGCACA GCTACAGCTC AGCCATGGAT 
GCAACGCCTA AGCATTATCT GCATGTGCCC AATGAACATC CTTACCTGAC CCCTTCTCCA 
GAGTCTCCAG AGCCCTGGTC CAGCCCTTCC CCTCACTGTG TGTCTGATTG GTCTGACTCC 
ACACCCAGCC CAGCTGTCAC AGCTCCTTCT CAAACCCAGA TTTCACAAGT CCAGGAGTCC 
AACGGGAAGA TGCAGGTGTT TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571624.2
CDS	237..7643
Translation	MGNYSLWIFLSILYLVHNSEGLLCVDTHRPCENGGMCIDSRCLCRPGYIGSLCQHLDPCHRSPCLNAAACKSQVANSIPQYTCVCQRGFRGQDCSLVDACATSPCANGARCTNWNNHYNCSCPPGYQGKNCRNDIDECRKPGKCLNGGICMNTHGSFRCECLTGYSGRTCEVPTQPCAPSQCLNGGTCHQTGDHTYECACLPGFRGHNCEDNVDDCPGHKCMNNGICVDGVNTYNCQCPPEWTGQYCAEDVNECLMQPNACHNGGTCFNTIGGHTCVCVNGWTGDDCSENIDDCATAVCFNGATCHDRVASFFCECPVGKTGLLCHLDDACVSNPCNEGAVCDTNPLNGRAICTCPAGFVGGACNQDMDECSIGANPCEHFGKCVNTEGSFQCQCGRGYTGPRCEIDINECLSMPCQNDATCLDRIGEFTCICMPGYQGKYCEVDIDECESNPCVNDGICRDMVNGFTCTCQPGFTGTMCQIDIDECASTPCQNGAKCIDRPNGYECRCAEGFEGRLCESNIDNCKPDPCHHGTCVDGIASYTCNCEPGYTGYRCENQLNECHSNPCQNGGKCVDLVNKYICQCQHGTSGTNCEINFDDCASNPCDYGICKDGINRYECVCKPGFTGPQCKDEIDECQSNPCRNGGTCVDDENGFHCQCPEGFHDPYCYSQVDECASNPCLHGTCRDDPNGYRCDCEPGWVGKNCDLDRNDCLPSPCQNAGTCFDQLNGFTCKCRQGFRGNLCQVNINECASSPCLNQGTCVDGVASFTCLCEPPYSGPTCAELLTPCSPNPCANHASCVHTPDYLGYQCNCQPGWQGHLCNNDINECTSNPCKNRGTCTNTLGGYVCSCRAGYTGPNCETDINDCSPNPCLNGGSCTDGVNSFRCSCLPGFTGARCATELNECQSVPCKNGGTCTDYVNSYTCTCKPGFTGLLCETNVPDCTESSCFNGGTCTDGINGFKCTCRTGFTGDYCQYEVNECDSQPCLNGGICQDAMESYRCSCPKGYTGPRCQYPVDWCRPSNPCKNGGRCRQKDASFTCDCLGGWSGRYCDIPGVSCEVAARQRGRQTDELCLHGGHCVNTGNTHYCKCPADYTGSYCESQFDHCEEKPCLNGATCRSYMGGFTCDCMPGYEGNNCEREVNECQSHPCQNGGTCIDLVGHYICSCPPGTLGVLCEINEDDCATPSWPRGMPKCQNNGTCVDRVGGYRCNCPPGFTGERCEGDINECLSNPCNPSNSLDCIQLPNDYQCVCKPGFTGRGCQSRFSVCESQPCKNGGVCSLSGSSPPGYSCTCQLGYAGSNCERSMNCKELPCYNGGSCTLTTRGARCTCIQGFGGPLCQHRSNDGCSSKPCHNGGLCTEETSYPFFHCQCTNGWKGKRCEQKTGSSAPLPSPCPIADCFSKANDGVCDKECNSLDCRWDGGDCSLAVNPWARCADPRCWRLFNNSQCDEFCNNAECLFDNFDCINKEKVCNPIYEAYCTDHYADGLCDQGCNTEECGWDGLDCARKIPEDLAEDMLVIVVLLPPEELLRTQTAFLQKLSAILRTTLRFRLDRNGDYMIRPYTGRETRIKRELNPQEVIGSIVYLEIDNRLCSQGSDDCFRNADSAAEYLGALSAREMLRFPYPIKEVTSEKREPSITEIPEWARLLLVGVASLFLLVILMVGMLIARRKREHSTLWFPEGFFLKKETSSNKNRREPVGQDSLGMKHMPKTVEESLLADHSDQWIDTDCPEAKRLKVEEPSILSDGEDAVDSRQWTQHHLAAADIRMPPSMALTPPQGEFDSDCMDVNVRGPDGFTPLMLASFCGGGLEPEVTEDDDSDESSANIISDLIYQGASLAAQTDRTGETALHLAARYARADAAKRLLDAGADANAQDNTGRSPLHAAVAADAQGVFQILIRNRATDLDARMYDGSTALILAARLAVEGMVEELITCHADVNAVDEIGKSALHWAAAVNNVEATIALLKNGANKDMQDLKEETPLFLAAREGSCEAVKVLLAHFANREITDHMDRLPRDIAQERMHHDIVQLLDEYNTVRSPQGHAGAGHHLSGSHTLSPLMCPPSNFLQGLKSTPQGKKNRRPGAKGIGGQHASGLKDSAKGRNKRLTLDMQSALLESSVTLSPVDSLDSPRGGASNAGYVTNPTSPAAMPSPGLFHSSMSVPNTPMVHSSILDSTSPFSVSLAQLSDLGDGGLSMQGRVGMQGGGVNPGPHNYVMNAGQMSLSMGMVSPVSVPFDWHNRMPPSSQCSQPMISMVHPVSSQAGMLPQTPTLQSSMLLHQQVFRNAQQPILQPTPVSSTPSISPVKLPPIAEQQPQQLHSHTLTNPNLSRMATSTPPTPQQAQPPPAFYQPQQPQAQPPQAPTAPQATQAQAIPSQAPPAQVSGSTAGPEDYPTPPSQHSYSSAMDATPKHYLHVPNEHPYLTPSPESPEPWSSPSPHCVSDWSDSTPSPAVTAPSQTQISQVQESNGKMQVFA

Target ORF information:

RefSeq Version	NM_131549.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio notch receptor 3 (notch3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131549.2

ORF Insert Sequence:

ATGGGGAATT ACAGCCTTTG GATATTTTTG TCAATTTTAT ATCTGGTGCA CAACAGCGAA 
GGATTGCTCT GCGTGGATAC ACACAGGCCG TGCGAAAATG GAGGAATGTG CATTGATTCA 
AGATGCTTAT GCCGGCCTGG TTATATTGGT TCGCTCTGTC AGCATCTGGA TCCCTGTCAC 
CGTTCGCCTT GTCTAAATGC AGCAGCTTGC AAGAGCCAAG TGGCGAATTC AATCCCTCAG 
TACACCTGTG TGTGTCAGCG AGGCTTTAGA GGTCAGGATT GTTCTCTCGT TGATGCGTGT 
GCCACAAGTC CTTGTGCCAA TGGTGCTCGT TGTACCAATT GGAATAATCA CTACAACTGC 
TCATGCCCAC CTGGGTATCA GGGAAAGAAC TGTCGCAATG ATATTGATGA GTGCCGCAAA 
CCCGGAAAGT GTCTTAATGG TGGCATCTGC ATGAATACAC ATGGCTCATT TCGCTGTGAG 
TGCCTTACAG GGTACAGCGG GCGCACATGC GAGGTGCCCA CCCAGCCCTG TGCCCCATCA 
CAATGTCTCA ACGGCGGCAC CTGCCATCAG ACCGGTGACC ACACCTACGA ATGTGCTTGT 
TTGCCAGGCT TCAGGGGACA CAATTGTGAG GACAATGTTG ATGACTGCCC TGGACACAAA 
TGCATGAATA ATGGGATCTG CGTCGATGGT GTCAACACAT ATAATTGCCA GTGCCCTCCT 
GAGTGGACAG GCCAGTACTG TGCTGAGGAT GTCAACGAGT GTCTCATGCA GCCCAATGCT 
TGTCACAATG GTGGTACATG CTTTAACACT ATTGGAGGGC ACACCTGTGT ATGCGTTAAT 
GGCTGGACTG GGGATGACTG CAGTGAAAAC ATTGACGACT GTGCCACAGC TGTCTGCTTC 
AACGGGGCCA CATGTCATGA CCGTGTGGCC TCCTTTTTCT GCGAGTGCCC TGTGGGCAAA 
ACTGGTTTAC TCTGTCATCT GGATGATGCT TGTGTCAGTA ACCCCTGTAA TGAGGGTGCG 
GTGTGTGACA CTAACCCACT GAATGGCCGG GCAATCTGCA CCTGCCCAGC TGGCTTTGTT 
GGTGGTGCCT GCAACCAAGA CATGGATGAA TGTTCCATTG GTGCCAACCC CTGTGAGCAT 
TTTGGGAAGT GTGTAAACAC TGAAGGCTCG TTCCAGTGCC AGTGTGGACG GGGCTACACT 
GGCCCTCGCT GCGAGATTGA TATCAACGAG TGTCTGTCCA TGCCTTGCCA AAACGATGCC 
ACCTGCCTGG ACCGCATTGG CGAGTTCACA TGCATCTGTA TGCCTGGCTA TCAGGGGAAG 
TACTGTGAGG TGGACATTGA TGAATGCGAG AGCAACCCCT GCGTAAATGA TGGCATCTGT 
CGAGATATGG TCAACGGCTT CACCTGCACC TGTCAGCCAG GGTTTACTGG CACCATGTGT 
CAAATCGACA TTGATGAGTG CGCCAGCACT CCTTGCCAGA ATGGAGCCAA GTGCATTGAC 
CGACCTAATG GATATGAATG CCGCTGTGCT GAAGGTTTTG AAGGAAGACT GTGTGAGAGC 
AACATTGATA ATTGTAAGCC TGACCCATGC CACCATGGTA CTTGCGTAGA TGGCATTGCC 
AGTTATACTT GCAACTGTGA GCCTGGCTAC ACTGGCTATC GCTGCGAAAA CCAGCTCAAT 
GAGTGCCATA GCAACCCCTG CCAGAATGGT GGCAAATGTG TTGACCTTGT CAACAAATAC 
ATCTGCCAGT GTCAACATGG CACTTCAGGT ACCAACTGTG AAATCAACTT TGACGACTGT 
GCTAGCAACC CTTGTGACTA CGGAATTTGC AAAGATGGCA TAAATCGCTA TGAGTGTGTC 
TGCAAACCTG GCTTTACCGG ACCTCAATGT AAGGATGAGA TAGATGAGTG CCAGTCGAAT 
CCCTGTCGCA ATGGAGGGAC CTGTGTAGAT GATGAGAATG GCTTCCACTG TCAGTGCCCT 
GAAGGGTTCC ATGATCCCTA CTGCTATTCA CAAGTGGATG AGTGTGCCAG CAATCCATGT 
TTACATGGGA CCTGCAGGGA TGACCCTAAT GGCTATCGGT GTGACTGTGA GCCTGGATGG 
GTGGGTAAGA ACTGTGACCT AGACCGAAAC GACTGTTTGC CCAGCCCCTG CCAGAATGCA 
GGCACCTGCT TTGACCAGCT CAATGGTTTC ACCTGCAAGT GTCGCCAAGG TTTTAGAGGT 
AACTTATGTC AGGTGAACAT CAATGAGTGT GCCTCCAGCC CCTGTCTAAA TCAGGGCACC 
TGTGTGGATG GAGTTGCTAG TTTCACCTGT CTCTGTGAAC CACCTTATAG TGGACCCACT 
TGTGCAGAAC TGCTCACACC TTGCTCTCCC AACCCCTGTG CCAACCATGC TTCCTGCGTG 
CACACACCTG ACTACCTAGG CTACCAGTGT AACTGCCAAC CAGGCTGGCA AGGCCATTTG 
TGCAACAATG ACATCAATGA ATGCACCTCT AACCCGTGTA AGAACCGTGG GACTTGCACA 
AATACCCTTG GAGGCTATGT GTGCTCCTGC CGTGCTGGCT ACACTGGACC CAATTGTGAA 
ACTGATATCA ATGACTGCTC GCCTAACCCC TGTCTGAATG GTGGATCCTG TACTGATGGA 
GTAAACTCAT TCCGCTGCAG TTGTCTGCCA GGCTTTACGG GGGCACGCTG TGCGACTGAG 
CTGAATGAAT GTCAGAGTGT ACCCTGCAAG AATGGTGGTA CCTGCACTGA TTATGTCAAC 
AGCTATACTT GTACCTGTAA ACCAGGCTTC ACAGGCCTTC TGTGTGAGAC CAACGTACCA 
GACTGCACTG AGAGCTCCTG TTTTAATGGG GGAACCTGCA CTGATGGGAT CAATGGTTTC 
AAATGCACCT GCCGCACAGG ATTCACTGGA GATTACTGCC AGTATGAAGT TAACGAGTGT 
GACTCTCAGC CATGCCTCAA TGGAGGAATT TGTCAAGATG CCATGGAGTC ATATCGCTGC 
TCATGTCCAA AAGGCTACAC TGGCCCACGT TGCCAGTATC CAGTTGATTG GTGCAGACCT 
TCTAACCCAT GCAAGAATGG TGGTCGCTGC AGACAAAAAG ATGCCTCCTT CACATGTGAC 
TGTTTGGGTG GCTGGTCAGG TCGTTATTGT GATATCCCAG GAGTGTCCTG TGAAGTCGCA 
GCCAGACAAA GAGGCCGCCA GACAGATGAG CTTTGCCTTC ACGGTGGACA CTGTGTCAAC 
ACTGGCAACA CGCACTACTG CAAATGTCCG GCAGACTACA CAGGCAGTTA CTGCGAGTCT 
CAGTTTGACC ACTGTGAGGA AAAACCTTGC CTCAATGGCG CCACCTGCAG AAGCTACATG 
GGAGGATTTA CTTGTGATTG TATGCCTGGT TATGAGGGGA ACAACTGTGA GAGAGAGGTC 
AATGAGTGCC AGTCTCACCC ATGTCAGAAT GGAGGAACCT GCATTGACTT AGTGGGACAT 
TACATCTGCT CCTGCCCTCC AGGAACACTG GGTGTACTCT GTGAGATAAA TGAGGATGAT 
TGTGCAACTC CCTCATGGCC TCGGGGAATG CCCAAATGTC AAAATAATGG CACATGTGTG 
GACAGAGTTG GAGGGTACAG ATGTAACTGT CCTCCAGGCT TCACTGGAGA ACGTTGCGAG 
GGAGACATCA ACGAATGTCT TTCCAACCCC TGCAACCCCT CAAACAGCTT GGACTGCATA 
CAGTTGCCCA ATGATTACCA ATGTGTGTGC AAACCTGGCT TTACAGGACG AGGGTGTCAA 
AGTAGATTCA GCGTGTGCGA GTCTCAGCCC TGTAAAAATG GAGGAGTATG TTCCTTGTCT 
GGCAGCTCCC CACCAGGATA CTCTTGCACT TGTCAGCTTG GGTACGCAGG TTCTAACTGT 
GAGAGGAGCA TGAACTGCAA AGAGCTGCCT TGCTACAATG GAGGCAGTTG CACTCTCACC 
ACACGAGGGG CACGTTGTAC TTGCATTCAG GGCTTTGGTG GGCCATTGTG TCAGCACCGC 
AGCAATGACG GCTGCTCCTC CAAGCCATGC CACAATGGAG GCTTATGTAC AGAGGAAACC 
AGCTATCCAT TTTTTCACTG CCAGTGCACC AATGGCTGGA AAGGCAAACG ATGTGAACAG 
AAAACTGGGT CATCGGCTCC ACTTCCTTCT CCTTGCCCTA TTGCTGACTG CTTCAGCAAG 
GCCAATGACG GTGTGTGTGA CAAAGAATGC AACTCCTTAG ACTGCCGCTG GGACGGAGGT 
GACTGTTCTC TTGCTGTAAA CCCCTGGGCA CGCTGTGCAG ACCCTCGATG CTGGCGGCTC 
TTCAATAACA GCCAATGTGA CGAGTTTTGC AATAACGCAG AGTGCCTGTT CGATAATTTC 
GACTGCATAA ACAAAGAGAA AGTCTGCAAC CCCATCTATG AAGCATACTG CACTGACCAT 
TATGCAGATG GACTCTGTGA TCAAGGATGC AATACTGAAG AATGTGGCTG GGATGGACTG 
GATTGTGCAA GGAAGATTCC AGAAGACCTT GCTGAAGATA TGCTTGTTAT TGTAGTCCTA 
CTGCCCCCAG AGGAGCTTCT AAGGACACAA ACAGCTTTCC TGCAGAAACT AAGTGCCATC 
CTACGCACCA CGTTGCGCTT CCGTCTCGAT CGAAATGGAG ATTACATGAT CCGACCTTAC 
ACAGGCCGTG AGACGCGCAT TAAACGGGAG CTTAATCCTC AGGAGGTCAT TGGGTCCATT 
GTATATTTGG AAATAGACAA CAGGCTGTGC TCTCAGGGCT CAGATGACTG CTTCCGCAAT 
GCTGACAGTG CTGCTGAATA CCTTGGTGCT CTGTCTGCTC GGGAGATGTT GCGCTTCCCA 
TACCCCATTA AAGAAGTGAC AAGTGAAAAA AGAGAGCCCT CGATTACTGA GATACCCGAA 
TGGGCTAGGC TACTGCTAGT AGGTGTAGCT TCTCTCTTCT TGTTAGTAAT CTTGATGGTT 
GGCATGTTGA TTGCCCGCCG CAAGCGTGAA CACAGCACAC TCTGGTTCCC TGAGGGCTTC 
TTCCTCAAAA AGGAAACAAG CAGTAACAAG AACCGCAGAG AACCTGTGGG CCAAGATTCA 
CTGGGCATGA AACACATGCC AAAGACAGTG GAAGAGTCTC TTCTGGCTGA TCACAGTGAC 
CAGTGGATAG ATACAGACTG CCCAGAGGCT AAGCGACTTA AGGTGGAAGA ACCAAGCATT 
CTGTCAGATG GTGAGGATGC AGTTGACAGC AGACAGTGGA CACAGCATCA CTTGGCAGCA 
GCAGATATCC GCATGCCACC TTCCATGGCA CTTACACCAC CACAAGGAGA GTTTGACAGT 
GACTGCATGG ATGTTAATGT CAGAGGCCCT GATGGCTTCA CACCTCTGAT GCTGGCATCT 
TTCTGTGGAG GAGGGCTTGA GCCAGAGGTG ACCGAAGATG ATGACTCAGA TGAATCCTCA 
GCCAACATCA TATCTGACCT CATTTACCAG GGAGCATCAC TTGCAGCTCA AACTGACCGC 
ACTGGAGAGA CTGCTCTGCA CCTGGCTGCA CGCTATGCCC GGGCAGATGC AGCTAAAAGA 
CTCCTGGATG CTGGGGCTGA TGCAAATGCA CAGGATAACA CAGGTCGCTC ACCTTTGCAT 
GCAGCTGTGG CAGCTGATGC ACAGGGAGTT TTCCAGATTC TGATCAGGAA CCGTGCCACA 
GACTTGGACG CGCGCATGTA TGATGGCTCC ACTGCTCTGA TACTTGCAGC CCGTCTGGCA 
GTAGAGGGCA TGGTGGAAGA GTTGATCACC TGCCATGCTG ATGTTAATGC TGTTGATGAA 
ATCGGAAAGT CAGCATTGCA CTGGGCAGCA GCAGTCAATA ACGTTGAAGC TACTATTGCC 
TTGTTGAAGA ATGGTGCCAA TAAAGATATG CAAGACCTCA AGGAGGAGAC TCCGCTGTTC 
TTGGCTGCCA GGGAAGGCAG CTGTGAGGCA GTAAAGGTGT TGTTGGCTCA CTTTGCCAAT 
AGGGAAATAA CAGATCACAT GGACAGACTC CCTCGAGATA TTGCCCAGGA GCGCATGCAT 
CATGACATTG TGCAGCTACT GGATGAATAT AACACTGTGC GAAGTCCACA GGGTCATGCA 
GGGGCAGGTC ACCACCTCTC TGGTAGCCAC ACACTCTCAC CGCTCATGTG CCCTCCCAGC 
AACTTCCTCC AAGGGCTGAA AAGTACCCCT CAGGGTAAGA AGAACCGTCG CCCGGGAGCT 
AAAGGCATTG GTGGACAGCA CGCTTCCGGC CTGAAAGATT CAGCCAAAGG ACGCAACAAA 
CGGCTGACTC TAGACATGCA GAGTGCTTTG CTGGAGAGCT CTGTCACGCT CTCCCCTGTT 
GACTCACTGG ATTCACCACG AGGAGGCGCC AGTAACGCTG GCTATGTCAC CAATCCAACC 
TCACCAGCTG CCATGCCCTC TCCCGGCCTT TTCCACTCCT CTATGTCTGT CCCTAATACC 
CCCATGGTGC ACAGCAGTAT CCTGGACAGC ACCAGCCCTT TTTCTGTCTC CCTGGCACAG 
TTGAGTGACC TGGGAGATGG TGGGCTCTCA ATGCAGGGGC GTGTGGGCAT GCAAGGAGGG 
GGTGTCAACC CAGGCCCCCA TAACTACGTG ATGAACGCCG GTCAAATGAG CCTTAGTATG 
GGCATGGTAA GTCCAGTAAG CGTGCCGTTT GACTGGCATA ACCGCATGCC TCCATCTTCT 
CAGTGCAGTC AGCCAATGAT CAGCATGGTG CATCCAGTAA GTAGTCAAGC AGGCATGCTC 
CCACAAACAC CAACCCTGCA AAGCAGCATG CTATTGCACC AGCAGGTGTT CCGTAATGCC 
CAGCAGCCCA TCCTGCAGCC CACACCTGTC TCCAGCACAC CGTCTATTAG TCCAGTCAAA 
CTGCCTCCCA TTGCTGAGCA GCAGCCGCAG CAACTTCACA GCCACACCCT CACCAACCCC 
AACCTCTCCC GCATGGCCAC CTCCACTCCC CCAACTCCAC AGCAGGCACA GCCTCCTCCA 
GCTTTCTACC AACCACAACA GCCACAAGCT CAGCCGCCGC AGGCTCCGAC AGCCCCTCAG 
GCAACGCAAG CACAAGCTAT TCCATCTCAG GCACCCCCAG CACAGGTGAG CGGCAGCACC 
GCAGGCCCAG AGGATTACCC CACACCACCT TCCCAGCACA GCTACAGCTC AGCCATGGAT 
GCAACGCCTA AGCATTATCT GCATGTGCCC AATGAACATC CTTACCTGAC CCCTTCTCCA 
GAGTCTCCAG AGCCCTGGTC CAGCCCTTCC CCTCACTGTG TGTCTGATTG GTCTGACTCC 
ACACCCAGCC CAGCTGTCAC AGCTCCTTCT CAAACCCAGA TTTCACAAGT CCAGGAGTCC 
AACGGGAAGA TGCAGGTGTT TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Cell reports27(5)1567-1578.e5(2019 04)
Xue Y,Liu D,Cui G,Ding Y,Ai D,Gao S,Zhang Y,Suo S,Wang X,Lv P,Zhou C,Li Y,Chen X,Peng G,Jing N,Han JJ,Liu F

The Progress of CRISPR/Cas9-Mediated Gene Editing in Generating Mouse/Zebrafish Models of Human Skeletal Diseases.
Computational and structural biotechnology journal17954-962(2019)
Wu N,Liu B,Du H,Zhao S,Li Y,Cheng X,Wang S,Lin J,Zhou J,,Qiu G,Wu Z,Zhang J

Rescue of hematopoietic stem/progenitor cells formation in plcg1 zebrafish mutant.
Scientific reports9(1)244(2019 Jan)
Ferri-Lagneau KF,Haider J,Sang S,Leung T

Notch Inhibition Promotes Differentiation of Liver Progenitor Cells into Hepatocytes via
Stem cells international20198451282(2019)
Russell JO,Ko S,Monga SP,Shin D

A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion.
eLife7(2018 11)
Naylor RW,Chang HG,Qubisi S,Davidson AJ

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notch3 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

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