kpnb1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	kpnb1
Entrez Gene ID	570700
Full Name	karyopherin (importin) beta 1
Synonyms	wu:fc12b10,zgc:123184
General protein information	Preferred Names karyopherin (importin) beta 1 Names importin subunit beta-1 karyopherin beta 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037702.3	NP_001032791.3	importin subunit beta-1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B05665g	XP_451788.1
Gallus gallus (chicken)	KPNB1	XP_004950071.1
Caenorhabditis elegans (roundworm)	imb-1	NP_491477.2
Homo sapiens (human)	KPNB1	NP_002256.2
Bos taurus (cattle)	KPNB1	NP_001180082.1
Mus musculus (house mouse)	Kpnb1	NP_032405.3
Drosophila melanogaster (fruit fly)	Fs(2)Ket	NP_001246109.1
Oryza sativa (rice)	Os12g0568800	NP_001067068.1
Neurospora crassa	NCU02011	XP_964876.1
Schizosaccharomyces pombe (fission yeast)	kap95	NP_594233.1
Saccharomyces cerevisiae (baker's yeast)	KAP95	NP_013451.1
Pan troglodytes (chimpanzee)	KPNB1	XP_003315429.1
Arabidopsis thaliana (thale cress)	AT5G53480	NP_200160.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009921	XP_319040.3
Xenopus tropicalis (tropical clawed frog)	kpnb1	XP_002940197.1
Magnaporthe oryzae (rice blast fungus)	MGG_03668	XP_003716218.1
Macaca mulatta (Rhesus monkey)	KPNB1	XP_001082833.2
Canis lupus familiaris (dog)	KPNB1	XP_005624657.1
Rattus norvegicus (Norway rat)	Kpnb1	NP_058759.1
Danio rerio (zebrafish)	kpnb1	NP_001032791.2
Eremothecium gossypii	AGOS_ACL187W	NP_983217.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

High level of GHR nuclear translocation in skeletal muscle of a hyperplasic transgenic zebrafish.
Figueiredo MA, Boyle RT, Sandrini JZ, Varela AS Jr, Marins LF
Journal of molecular endocrinology56(1)47-54(2016 Jan)

Genome wide screens in yeast to identify potential binding sites and target genes of DNA-binding proteins.
Zeng J, Yan J, Wang T, Mosbrook-Davis D, Dolan KT, Christensen R, Stormo GD, Haussler D, Lathrop RH, Brachmann RK, Burgess SM
Nucleic acids research36(1)e8(2008 Jan)

Requirement of nuclear localization and transcriptional activity of p53 for its targeting to the yolk syncytial layer (YSL) nuclei in zebrafish embryo and its use for apoptosis assay.
Chen GD, Chou CM, Hwang SP, Wang FF, Chen YC, Hung CC, Chen JY, Huang CJ
Biochemical and biophysical research communications344(1)272-82(2006 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following kpnb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kpnb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa46011	NM_001037702.3 Latest version!	Danio rerio karyopherin (importin) beta 1 (kpnb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kpnb1 ( NM_001037702.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001032791.3 Danio rerio karyopherin (importin) beta 1 (kpnb1), mRNA.

Price & Availability↑

CloneID	ODa46011
Clone ID Related Accession (Same CDS sequence)	NM_001037702.3
Accession Version	NM_001037702.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	importin subunit beta-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU929079.6. On Nov 19, 2014 this sequence version replaced NM_001037702.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQQ01002210.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGA TCACGATCCT CGAGAAGACC GTGTCTCCAG ATCGGAATGA GCTGGAGGCG 
GCACAGAAGT TTCTGGAGCA GGCGGCCATC GAGAACCTGC CGACCTTCCT GGTGGAGTTG 
TCCAAGGTGT TGGCGAACCC GGGGAACACA CAGGTGGCTC GCGTCGCCGC CGGCCTGCAG 
GTCAAGAACT CTTTGACCTC TAAAGACCCG GAAGTGAAGA GTCAGTACCA GCAGAGATGG 
CTGGCTATCG ACACCAGCGC ACGCAGGGAG ATCAAGAACT ACGTGCTGCA GACGCTGGGC 
ACAGAGACGT ACCGGCCCAG CTCCGCATCT CAGTGTGTGG CGGGCATCGC CTGCGCAGAG 
ATCCCAGTAA ACCAGTGGCC GGAGCTGATC CCACAGCTGG TGGCCAACGT GACGGACCCC 
AGCAGCACCG AACACATGAA GGAGTCCACG CTGGAGGCCA TCGGCTACAT CTGCCAGGAC 
ATAGACCCAG AGCACCTGCA GGACAGCGCC AACCAGATCC TGACCGCCAT CATCCAGGGC 
ATGAGGAAAG AGGAGCCCAG CAACAACGTC AAGCTGGCCG CCACCAACGC GCTGCTCAAC 
TCGCTGGAGT TCACCAAAGC CAACTTCGAC AAGGAGACGG AGAGACACTT CATCATGCAG 
GTGGTGTGTG AAGCCACGCA GTGTCCAGAC ACCAGGGTGA GAGTAGCTGC GTTACAGAAC 
CTGGTGAAGA TCATGTCCCT GTATTACCAG TACATGGAGA CCTACATGGG TCCTGCACTG 
TTCGCCATCA CGATAGAGGC CATGAAGAGC GACATCGATG AGGTGGCCCT GCAGGGAATC 
GAGTTCTGGT CCAACGTGTG TGATGAGGAG ATGGATCTGG CCATTGAGGC CACGGAGGCC 
TCAGAGCAGG GCCGACCGCC GGAGCACACC AGCAAGTTTT ACGCTAAAGG AGCCCTGCAG 
TACCTGGTGC CCATCCTGAC GCAGACGCTC ACTAAACAGG ATGAAAACGA TGACGATGAC 
GACTGGAACC CGTGTAAGGC CGCAGGAGTG TGTCTGATGC TGCTGGCCAC CTGCTGTGAG 
GATGATGTGG TGCCGCACGT GCTGCCCTTC ATCAAGGAGC ACATCAAACA CCCCGACTGG 
CGCTACCGCG ACGCCTCCGT CATGGCCTTC GGCTCTATCC TGGAGGGTCC TGAACTCAAC 
CAGCTCAAAC CACTCGTCAT CCAGGCGATG CCCACGCTGA TCGAGCTGAT GAAAGACCCC 
AGTGTGGTGG TGCGGGACAC GACGGCCTGG ACTCTGGGCC GCATCTGTGA TCTGCTGCCT 
GAAGCCGCCA TCAATGAGGT GTATCTGAGC CCGCTGCTGC AGTGCCTGAT CGAGGGGCTG 
GGGGCCGAAC CACGCGTCGC CTCCAACGTC TGCTGGGCTT TCTCCAGTCT GGCTGAGGCG 
GCGTATGAGG CCACAGATGT GGCGGATGAT CAGGAGGAGC CGAGCACTTA CTGCTTGTCC 
TCCTCCTTCG AGCTGATCGT GCAGAAGCTG CTGGAGACCA CCGACAGGCC GGACGGGCAC 
CAGAATAACC TGCGCAGCGC GGCGTACGAG GCTCTGATGG AGATCGTGAA GAACAGCGCG 
AAGGATTGTT ACCCGGCGGT GCAGAAGACC ACGCTGGTCA TCATGGAGCG GCTGCAGCAG 
GTCCTGCAAA TGGAGTCTCA CATCCAGAGC ACATCAGACC GAATCCAGTT CAATGACCTC 
CAGTCTCTGC TGTGCGCCAC GCTACAGAAC GTGCTGAGGA AGGTGCAGCA TCAGGATGCG 
CTGCAGATCT CGGATGTGGT GATGGCGTCG CTGCTGCGCA TGTTTCAGAG CACCGCCGGC 
TCCGGAGGCG TGCAGGAGGA CGCACTGATG GCCGTCAGCA CACTGGTGGA GGTTTTGGGC 
AGTGACTTCC TGAAGTACAT GGACGCCTTC AAGCCTTTCC TGATTATCGG ACTGAAGAAT 
TATGCAGAAT ATCAGGTCTG CCTGGCTGCG GTGGGACTGG TGTGTGATCT GTGTCGGGCT 
CTGATGGCCA ATATTCTGCC TTACTGTGAT GAAATCATGC AGCTGCTGCT GGAGAATCTG 
GGTAATGAGA ATGTGCACCG CTCCGTCAAG CCCCAGATTC TGTCTGTGTT TGGAGACATC 
GCTCTCGCCA TTGGAGGAGA ATTCAAGAAG TACCTGGACA TTGTGCTGGA CACTCTGCAG 
CAGGCCTCGC AGGCACAGGT GGACAAGACG GACTATGATA TGGTGGATTA CCTGAACGAG 
CTGCGTGAGG GCTGTCTGGA GGCCTACACC GGCATCATCC AGGGCCTGAA GGGGGACCAG 
GAGAATGTGC ACCCCGATGT GATGCTGGTT CAGCCGCGTG TGGAGTTCAT CTTGTCCTTC 
ATTCACCACA TCGCAGAGGA CGAGGACCGA TCTGACGGAG TCGTGGCTAA TGCTGTAGGG 
CTCATCGGGG ACCTGTGCAC CACCTTCGGG AAGGATGTGA TGAAGCTGGT GGAGGTGCGT 
CCTCAGATCA ATGACCTGCT GACTGAAGGC CGGAGATCCA AAACCAGCAA AACCAAGACT 
CTGGCCACGT GGGCCGCCAA GGAGCTGCGC AAGCTGAAGA GCCAAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032791.3
CDS	153..2783
Translation	MELITILEKTVSPDRNELEAAQKFLEQAAIENLPTFLVELSKVLANPGNTQVARVAAGLQVKNSLTSKDPEVKSQYQQRWLAIDTSARREIKNYVLQTLGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTDPSSTEHMKESTLEAIGYICQDIDPEHLQDSANQILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKETERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEATEASEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDVVPHVLPFIKEHIKHPDWRYRDASVMAFGSILEGPELNQLKPLVIQAMPTLIELMKDPSVVVRDTTAWTLGRICDLLPEAAINEVYLSPLLQCLIEGLGAEPRVASNVCWAFSSLAEAAYEATDVADDQEEPSTYCLSSSFELIVQKLLETTDRPDGHQNNLRSAAYEALMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGSDFLKYMDAFKPFLIIGLKNYAEYQVCLAAVGLVCDLCRALMANILPYCDEIMQLLLENLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLDIVLDTLQQASQAQVDKTDYDMVDYLNELREGCLEAYTGIIQGLKGDQENVHPDVMLVQPRVEFILSFIHHIAEDEDRSDGVVANAVGLIGDLCTTFGKDVMKLVEVRPQINDLLTEGRRSKTSKTKTLATWAAKELRKLKSQA

Target ORF information:

RefSeq Version	NM_001037702.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio karyopherin (importin) beta 1 (kpnb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037702.3

ORF Insert Sequence:

ATGGAGCTGA TCACGATCCT CGAGAAGACC GTGTCTCCAG ATCGGAATGA GCTGGAGGCG 
GCACAGAAGT TTCTGGAGCA GGCGGCCATC GAGAACCTGC CGACCTTCCT GGTGGAGTTG 
TCCAAGGTGT TGGCGAACCC GGGGAACACA CAGGTGGCTC GCGTCGCCGC CGGCCTGCAG 
GTCAAGAACT CTTTGACCTC TAAAGACCCG GAAGTGAAGA GTCAGTACCA GCAGAGATGG 
CTGGCTATCG ACACCAGCGC ACGCAGGGAG ATCAAGAACT ACGTGCTGCA GACGCTGGGC 
ACAGAGACGT ACCGGCCCAG CTCCGCATCT CAGTGTGTGG CGGGCATCGC CTGCGCAGAG 
ATCCCAGTAA ACCAGTGGCC GGAGCTGATC CCACAGCTGG TGGCCAACGT GACGGACCCC 
AGCAGCACCG AACACATGAA GGAGTCCACG CTGGAGGCCA TCGGCTACAT CTGCCAGGAC 
ATAGACCCAG AGCACCTGCA GGACAGCGCC AACCAGATCC TGACCGCCAT CATCCAGGGC 
ATGAGGAAAG AGGAGCCCAG CAACAACGTC AAGCTGGCCG CCACCAACGC GCTGCTCAAC 
TCGCTGGAGT TCACCAAAGC CAACTTCGAC AAGGAGACGG AGAGACACTT CATCATGCAG 
GTGGTGTGTG AAGCCACGCA GTGTCCAGAC ACCAGGGTGA GAGTAGCTGC GTTACAGAAC 
CTGGTGAAGA TCATGTCCCT GTATTACCAG TACATGGAGA CCTACATGGG TCCTGCACTG 
TTCGCCATCA CGATAGAGGC CATGAAGAGC GACATCGATG AGGTGGCCCT GCAGGGAATC 
GAGTTCTGGT CCAACGTGTG TGATGAGGAG ATGGATCTGG CCATTGAGGC CACGGAGGCC 
TCAGAGCAGG GCCGACCGCC GGAGCACACC AGCAAGTTTT ACGCTAAAGG AGCCCTGCAG 
TACCTGGTGC CCATCCTGAC GCAGACGCTC ACTAAACAGG ATGAAAACGA TGACGATGAC 
GACTGGAACC CGTGTAAGGC CGCAGGAGTG TGTCTGATGC TGCTGGCCAC CTGCTGTGAG 
GATGATGTGG TGCCGCACGT GCTGCCCTTC ATCAAGGAGC ACATCAAACA CCCCGACTGG 
CGCTACCGCG ACGCCTCCGT CATGGCCTTC GGCTCTATCC TGGAGGGTCC TGAACTCAAC 
CAGCTCAAAC CACTCGTCAT CCAGGCGATG CCCACGCTGA TCGAGCTGAT GAAAGACCCC 
AGTGTGGTGG TGCGGGACAC GACGGCCTGG ACTCTGGGCC GCATCTGTGA TCTGCTGCCT 
GAAGCCGCCA TCAATGAGGT GTATCTGAGC CCGCTGCTGC AGTGCCTGAT CGAGGGGCTG 
GGGGCCGAAC CACGCGTCGC CTCCAACGTC TGCTGGGCTT TCTCCAGTCT GGCTGAGGCG 
GCGTATGAGG CCACAGATGT GGCGGATGAT CAGGAGGAGC CGAGCACTTA CTGCTTGTCC 
TCCTCCTTCG AGCTGATCGT GCAGAAGCTG CTGGAGACCA CCGACAGGCC GGACGGGCAC 
CAGAATAACC TGCGCAGCGC GGCGTACGAG GCTCTGATGG AGATCGTGAA GAACAGCGCG 
AAGGATTGTT ACCCGGCGGT GCAGAAGACC ACGCTGGTCA TCATGGAGCG GCTGCAGCAG 
GTCCTGCAAA TGGAGTCTCA CATCCAGAGC ACATCAGACC GAATCCAGTT CAATGACCTC 
CAGTCTCTGC TGTGCGCCAC GCTACAGAAC GTGCTGAGGA AGGTGCAGCA TCAGGATGCG 
CTGCAGATCT CGGATGTGGT GATGGCGTCG CTGCTGCGCA TGTTTCAGAG CACCGCCGGC 
TCCGGAGGCG TGCAGGAGGA CGCACTGATG GCCGTCAGCA CACTGGTGGA GGTTTTGGGC 
AGTGACTTCC TGAAGTACAT GGACGCCTTC AAGCCTTTCC TGATTATCGG ACTGAAGAAT 
TATGCAGAAT ATCAGGTCTG CCTGGCTGCG GTGGGACTGG TGTGTGATCT GTGTCGGGCT 
CTGATGGCCA ATATTCTGCC TTACTGTGAT GAAATCATGC AGCTGCTGCT GGAGAATCTG 
GGTAATGAGA ATGTGCACCG CTCCGTCAAG CCCCAGATTC TGTCTGTGTT TGGAGACATC 
GCTCTCGCCA TTGGAGGAGA ATTCAAGAAG TACCTGGACA TTGTGCTGGA CACTCTGCAG 
CAGGCCTCGC AGGCACAGGT GGACAAGACG GACTATGATA TGGTGGATTA CCTGAACGAG 
CTGCGTGAGG GCTGTCTGGA GGCCTACACC GGCATCATCC AGGGCCTGAA GGGGGACCAG 
GAGAATGTGC ACCCCGATGT GATGCTGGTT CAGCCGCGTG TGGAGTTCAT CTTGTCCTTC 
ATTCACCACA TCGCAGAGGA CGAGGACCGA TCTGACGGAG TCGTGGCTAA TGCTGTAGGG 
CTCATCGGGG ACCTGTGCAC CACCTTCGGG AAGGATGTGA TGAAGCTGGT GGAGGTGCGT 
CCTCAGATCA ATGACCTGCT GACTGAAGGC CGGAGATCCA AAACCAGCAA AACCAAGACT 
CTGGCCACGT GGGCCGCCAA GGAGCTGCGC AAGCTGAAGA GCCAAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

High level of GHR nuclear translocation in skeletal muscle of a hyperplasic transgenic zebrafish.
Journal of molecular endocrinology56(1)47-54(2016 Jan)
Figueiredo MA,Boyle RT,Sandrini JZ,Varela AS Jr,Marins LF

Genome wide screens in yeast to identify potential binding sites and target genes of DNA-binding proteins.
Nucleic acids research36(1)e8(2008 Jan)
Zeng J,Yan J,Wang T,Mosbrook-Davis D,Dolan KT,Christensen R,Stormo GD,Haussler D,Lathrop RH,Brachmann RK,Burgess SM

Requirement of nuclear localization and transcriptional activity of p53 for its targeting to the yolk syncytial layer (YSL) nuclei in zebrafish embryo and its use for apoptosis assay.
Biochemical and biophysical research communications344(1)272-82(2006 May)
Chen GD,Chou CM,Hwang SP,Wang FF,Chen YC,Hung CC,Chen JY,Huang CJ

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kpnb1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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