ANAPC1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANAPC1
Entrez Gene ID	568795
Full Name	anaphase promoting complex subunit 1
Synonyms	si:ch211-160m22.1
General protein information	Preferred Names anaphase promoting complex subunit 1 Names anaphase-promoting complex subunit 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021480904.1	XP_021336579.1	anaphase-promoting complex subunit 1 isoform X4
XM_005157066.3	XP_005157123.1	anaphase-promoting complex subunit 1 isoform X1
XM_005157066.2	XP_005157123.1	anaphase-promoting complex subunit 1 isoform X1
XM_005157067.2	XP_005157124.1	anaphase-promoting complex subunit 1 isoform X3
XM_005157067.3	XP_005157124.1	anaphase-promoting complex subunit 1 isoform X3
XM_001921133.4	XP_001921168.2	anaphase-promoting complex subunit 1 isoform X2
XM_001921133.3	XP_001921168.2	anaphase-promoting complex subunit 1 isoform X2
XM_017358856.1	XP_017214345.1	anaphase-promoting complex subunit 1 isoform X4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ANAPC1	XP_005630492.1
Rattus norvegicus (Norway rat)	Anapc1	NP_001101241.1
Arabidopsis thaliana (thale cress)	EMB2771	NP_196175.1
Oryza sativa (rice)	Os05g0354300	NP_001055285.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000374	XP_310737.5
Drosophila melanogaster (fruit fly)	shtd	NP_573025.2
Xenopus tropicalis (tropical clawed frog)	anapc1	NP_001072866.1
Homo sapiens (human)	ANAPC1	NP_073153.1
Mus musculus (house mouse)	Anapc1	NP_032595.2
Danio rerio (zebrafish)	anapc1	XP_001921168.2
Bos taurus (cattle)	ANAPC1	XP_005212426.1
Gallus gallus (chicken)	ANAPC1	XP_419303.3

Gene Ontology

The following ANAPC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANAPC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa54779	XM_017358856.1 Latest version!	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa148661	XM_021480904.1 Latest version!	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa19121	XM_005157066.3 Latest version!	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa19122	XM_001921133.4 Latest version!	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa19123	XM_005157067.3 Latest version!	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa19121	XM_005157066.2	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa19123	XM_005157067.2	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa19122	XM_001921133.3	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANAPC1 ( XM_017358856.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214345.1 Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa54779
Clone ID Related Accession (Same CDS sequence)	XM_017358856.1
Accession Version	XM_017358856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	anaphase-promoting complex subunit 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGCAGG 
GAACCTCTCC CGACTTTGTT CAGCATGCTG CATCCATTAG ATGAAATCGC TCCTGTTGTT 
TGTAAGTCCA CCGGTCTGTT TGAAACCCGG CTCCAGTATG TTTCAGACAG CGCTCTGAGA 
GTGGTGTTCA CCTGCCTCAA ACCCTCCATT ATCATGACCT ACGACACACA GCAGTGCACA 
CACACCGTCT GGAATCTGCG CAAAGTCACA TCTGAGGAGC AGAACACTGT GTTAAAGTTT 
CCAGATCAGT CTGGACCGGC TCACACTCCT AATGCGGCTC CCAGTTTCCT CAGCACACAC 
CTGAGAAACG TGAACCGGCT GGACTCTCCG GCTTCACCTT TACACTGCCA TGCTCTTCAC 
AACCAAAGCC GAATCCCAGC CTCCCCGGCC CTGCACTCTC GGGTTCACTC GCCCAGCATT 
TCCAACATGG CTGCTCTCAG TCGTGCTCAT TCCCCTGGCA TCGCCGTGCC CTCGTTCTCT 
GGGAATCAGC GTTTCAACAT GTCATGCCAC ACGCCATCTT CGCGTGGCCA CAGCATCCTG 
GCTTCACCCA ACAGCACCCT GAATGACACC CTGCTGCCTG AGATGGAGCC CATCTTACCA 
GACCTCTGCA TCGAGCAGCT GTGGACGGAA ACCAGCGCCA TCTCCAGGGA GAAAGGCTGC 
CAGGCGTCTA AAGTCTTCAT CACCTCTGAT CTGTGTGAAA ACCGGTACCT GTGCTTCCTG 
GTCGAGTCCC ATCAGCAGCT CAGGTGTGTA AAATTTCTTG AGAGCAATGA AACGTCTCAA 
CTGATCTTTG CAACGGTTTC CACCATCGCT GCAAAAGATG CCGCTCCTCT AGAGGACATT 
CACACAATGC TGGTGCTGGA GGCCAATGGG AGTCTGGTGT TGTACACAGG AGTCACCAGG 
GTCAGCAAGG TGTTTGTCCC TGGCCTTCTG TCTCCTACAT TCAGCGTGCC GAACCAGCCG 
CCAATATTGA GCACACCTCT GGAAAACATC AGCACTCCGG CCCGAGCCAC AGTTCAGCAT 
ATGAGCCGTC TGGAGGAGGT TGGCATGCCG TCTCCTGTGC TGGAGATGCG CAGCTCGTTG 
CAGCACAATG AGTCCTCATG GCTGGAAGAG TCTCCATTTC AGCAAACTGC TCCTCGTATT 
CAGACCCTCC GAGACCCCGT CTGCAACAGA GTCACACTGG AATTGAGTTC CGGTTCCATG 
TTGAGAATCA CCATTCCCGA GGTGGCCACT TCAGAGCTGG TCAAACGCTG CCTGCAGGCC 
ATCCGATTTA TTTTACCCAA GGACACTGCC ATGAAAGTGC TGGTCAAGTG GTACAACAGC 
TACAACGCCC CCGGAGGTCC CAGCATGCAC CTGGAGTGGA ACCTGTTCGT CACCTGCCTA 
ATGACAATGA TGGGTTACAA CACAGAGCGG CTGACGTGGA CACGCAACCT TCATTTTGAA 
GTGCCTCTTT CACCAGTAAT CGCTGCTAAG AAAGCCCGTC CTGCCGACAC TGGGTCAGAT 
GAGGATTGGC TGTATCTCCG CTGCTCAGAC TATCATTGGC AGGTGTCCAT GCGGCCTGCG 
GTGTCTGTTC AGGGTCTGGA GATGCTGTTT CCCCCTGCTG GAGTGGATGA TCAGGAGTCT 
GCTCTGGGTT TGGAGTCGTC CGCTCTCCTT TTCCCACACA TCCCGCTGCT CTTTTATGTC 
CTGCATCTGC TGTATGAGGA CCTGAAGCTG GACGAGTTGC AGAGGAGTGG TGCCAGGGCT 
CTGGTTGGCC TGCTGCAACA GCTTGCCAGG GACCTGCAGC TGGAGGATTT CATTGATCTG 
TACTGGCGTG ATTTCCCCTC ATTCATCAAA ACGTTCAAGG AGGCCTGTGT GATTGATCAA 
GCTCAGATGG AGCTGATGAC GTGGCCGGTG TTTCTGAATG GAGAAGCTCC GTGTGTGTTC 
AACTGGATCA GTCGCTGTCT GAGAGGACAA GATGTTGCGC CGTTTCCATA TCTGCCTGGC 
ATCTGTGAGA GAACCAAACT ACTGGTGCTG AGTTATGCCC TGTACGTGGT GGGAGATGAA 
GACGCCTGCT CCACCAACAT GCTCAAATAC CTTTGCAGAC TGTCAGCAGG GCAAAAGAAG 
AATGCAAGTG ATCCATTTCT GAGAAGGCAG AGCAGTGTGA AGCCCATATC TTCATCCGGA 
TGCCTGGCTG AGAAGCTGGT GCTGTGGTTA ACCTCCGCAG GATTTACACT GAAGGATCTC 
GAGACGGTGC CGTTCGGTGT GGCTCTGCCA ATTAGAGACG CCATCTATCA GTGCAGAGAA 
CACCCCTGCT CTGATTGGTC CGAAGAGGTC TGCGCTCTGA TTGGCCGACA GGACCTGACC 
AGACAAGCTC ACAAAGTCAC TCTAGCCAAT CGTAAATCGA CGGTGGGTCT TAACGTGATG 
CTGGAGCCGC CGGTGAGCAG CGAAGCCGAT GAGGAAGAGG ACGGCATGAC GGATCTTCAC 
CCCGAGGTGA CCGCGCTCAT CTGGAGTCAA GATCTCAGGG TGCAGGAGGT GCGGCGGCTG 
CTCCAGAGCT CCAGACCCGT CCGTGTTAAT GTGCCACATC TGCCGGAGAT CAGTGACCAC 
GAGTACATTG AGGAGAAGGA GAACAAGCTC CTCCAGCTGT GCCAGCGCAC CATGTCTCTC 
CCAGTGGGAC GAGGGCTTTT TACTCTCTTC TCATACCACC CTGTGCCAAC CGAACCACTG 
CCTGTGCCCA AACTCAACCT GACTGGTACT CTCAGAAACG CACACCCATG CTTTAAGCTC 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214345.1
CDS	216..3581
Translation	MSHVYEERATMIAAGELQSFVPFGREHCKNHPNAVNLQLRQLQPASELWSSDGAAGLVGSLQEVTLQERDKERWQLRKGTAPDDVEYDEELYVAGNMVIWSRGSKNQASCVYKAFTVDSPVQQALWCNFTVPQAKKDVKDVVEQTVCIVQSTCVNVHSMSGKDFISPLPFQVSALWPTKFGLLLERKNITVDGHMSPISREPLPTLFSMLHPLDEIAPVVCKSTGLFETRLQYVSDSALRVVFTCLKPSIIMTYDTQQCTHTVWNLRKVTSEEQNTVLKFPDQSGPAHTPNAAPSFLSTHLRNVNRLDSPASPLHCHALHNQSRIPASPALHSRVHSPSISNMAALSRAHSPGIAVPSFSGNQRFNMSCHTPSSRGHSILASPNSTLNDTLLPEMEPILPDLCIEQLWTETSAISREKGCQASKVFITSDLCENRYLCFLVESHQQLRCVKFLESNETSQLIFATVSTIAAKDAAPLEDIHTMLVLEANGSLVLYTGVTRVSKVFVPGLLSPTFSVPNQPPILSTPLENISTPARATVQHMSRLEEVGMPSPVLEMRSSLQHNESSWLEESPFQQTAPRIQTLRDPVCNRVTLELSSGSMLRITIPEVATSELVKRCLQAIRFILPKDTAMKVLVKWYNSYNAPGGPSMHLEWNLFVTCLMTMMGYNTERLTWTRNLHFEVPLSPVIAAKKARPADTGSDEDWLYLRCSDYHWQVSMRPAVSVQGLEMLFPPAGVDDQESALGLESSALLFPHIPLLFYVLHLLYEDLKLDELQRSGARALVGLLQQLARDLQLEDFIDLYWRDFPSFIKTFKEACVIDQAQMELMTWPVFLNGEAPCVFNWISRCLRGQDVAPFPYLPGICERTKLLVLSYALYVVGDEDACSTNMLKYLCRLSAGQKKNASDPFLRRQSSVKPISSSGCLAEKLVLWLTSAGFTLKDLETVPFGVALPIRDAIYQCREHPCSDWSEEVCALIGRQDLTRQAHKVTLANRKSTVGLNVMLEPPVSSEADEEEDGMTDLHPEVTALIWSQDLRVQEVRRLLQSSRPVRVNVPHLPEISDHEYIEEKENKLLQLCQRTMSLPVGRGLFTLFSYHPVPTEPLPVPKLNLTGTLRNAHPCFKLL

Target ORF information:

RefSeq Version	XM_017358856.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358856.1

ORF Insert Sequence:

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGCAGG 
GAACCTCTCC CGACTTTGTT CAGCATGCTG CATCCATTAG ATGAAATCGC TCCTGTTGTT 
TGTAAGTCCA CCGGTCTGTT TGAAACCCGG CTCCAGTATG TTTCAGACAG CGCTCTGAGA 
GTGGTGTTCA CCTGCCTCAA ACCCTCCATT ATCATGACCT ACGACACACA GCAGTGCACA 
CACACCGTCT GGAATCTGCG CAAAGTCACA TCTGAGGAGC AGAACACTGT GTTAAAGTTT 
CCAGATCAGT CTGGACCGGC TCACACTCCT AATGCGGCTC CCAGTTTCCT CAGCACACAC 
CTGAGAAACG TGAACCGGCT GGACTCTCCG GCTTCACCTT TACACTGCCA TGCTCTTCAC 
AACCAAAGCC GAATCCCAGC CTCCCCGGCC CTGCACTCTC GGGTTCACTC GCCCAGCATT 
TCCAACATGG CTGCTCTCAG TCGTGCTCAT TCCCCTGGCA TCGCCGTGCC CTCGTTCTCT 
GGGAATCAGC GTTTCAACAT GTCATGCCAC ACGCCATCTT CGCGTGGCCA CAGCATCCTG 
GCTTCACCCA ACAGCACCCT GAATGACACC CTGCTGCCTG AGATGGAGCC CATCTTACCA 
GACCTCTGCA TCGAGCAGCT GTGGACGGAA ACCAGCGCCA TCTCCAGGGA GAAAGGCTGC 
CAGGCGTCTA AAGTCTTCAT CACCTCTGAT CTGTGTGAAA ACCGGTACCT GTGCTTCCTG 
GTCGAGTCCC ATCAGCAGCT CAGGTGTGTA AAATTTCTTG AGAGCAATGA AACGTCTCAA 
CTGATCTTTG CAACGGTTTC CACCATCGCT GCAAAAGATG CCGCTCCTCT AGAGGACATT 
CACACAATGC TGGTGCTGGA GGCCAATGGG AGTCTGGTGT TGTACACAGG AGTCACCAGG 
GTCAGCAAGG TGTTTGTCCC TGGCCTTCTG TCTCCTACAT TCAGCGTGCC GAACCAGCCG 
CCAATATTGA GCACACCTCT GGAAAACATC AGCACTCCGG CCCGAGCCAC AGTTCAGCAT 
ATGAGCCGTC TGGAGGAGGT TGGCATGCCG TCTCCTGTGC TGGAGATGCG CAGCTCGTTG 
CAGCACAATG AGTCCTCATG GCTGGAAGAG TCTCCATTTC AGCAAACTGC TCCTCGTATT 
CAGACCCTCC GAGACCCCGT CTGCAACAGA GTCACACTGG AATTGAGTTC CGGTTCCATG 
TTGAGAATCA CCATTCCCGA GGTGGCCACT TCAGAGCTGG TCAAACGCTG CCTGCAGGCC 
ATCCGATTTA TTTTACCCAA GGACACTGCC ATGAAAGTGC TGGTCAAGTG GTACAACAGC 
TACAACGCCC CCGGAGGTCC CAGCATGCAC CTGGAGTGGA ACCTGTTCGT CACCTGCCTA 
ATGACAATGA TGGGTTACAA CACAGAGCGG CTGACGTGGA CACGCAACCT TCATTTTGAA 
GTGCCTCTTT CACCAGTAAT CGCTGCTAAG AAAGCCCGTC CTGCCGACAC TGGGTCAGAT 
GAGGATTGGC TGTATCTCCG CTGCTCAGAC TATCATTGGC AGGTGTCCAT GCGGCCTGCG 
GTGTCTGTTC AGGGTCTGGA GATGCTGTTT CCCCCTGCTG GAGTGGATGA TCAGGAGTCT 
GCTCTGGGTT TGGAGTCGTC CGCTCTCCTT TTCCCACACA TCCCGCTGCT CTTTTATGTC 
CTGCATCTGC TGTATGAGGA CCTGAAGCTG GACGAGTTGC AGAGGAGTGG TGCCAGGGCT 
CTGGTTGGCC TGCTGCAACA GCTTGCCAGG GACCTGCAGC TGGAGGATTT CATTGATCTG 
TACTGGCGTG ATTTCCCCTC ATTCATCAAA ACGTTCAAGG AGGCCTGTGT GATTGATCAA 
GCTCAGATGG AGCTGATGAC GTGGCCGGTG TTTCTGAATG GAGAAGCTCC GTGTGTGTTC 
AACTGGATCA GTCGCTGTCT GAGAGGACAA GATGTTGCGC CGTTTCCATA TCTGCCTGGC 
ATCTGTGAGA GAACCAAACT ACTGGTGCTG AGTTATGCCC TGTACGTGGT GGGAGATGAA 
GACGCCTGCT CCACCAACAT GCTCAAATAC CTTTGCAGAC TGTCAGCAGG GCAAAAGAAG 
AATGCAAGTG ATCCATTTCT GAGAAGGCAG AGCAGTGTGA AGCCCATATC TTCATCCGGA 
TGCCTGGCTG AGAAGCTGGT GCTGTGGTTA ACCTCCGCAG GATTTACACT GAAGGATCTC 
GAGACGGTGC CGTTCGGTGT GGCTCTGCCA ATTAGAGACG CCATCTATCA GTGCAGAGAA 
CACCCCTGCT CTGATTGGTC CGAAGAGGTC TGCGCTCTGA TTGGCCGACA GGACCTGACC 
AGACAAGCTC ACAAAGTCAC TCTAGCCAAT CGTAAATCGA CGGTGGGTCT TAACGTGATG 
CTGGAGCCGC CGGTGAGCAG CGAAGCCGAT GAGGAAGAGG ACGGCATGAC GGATCTTCAC 
CCCGAGGTGA CCGCGCTCAT CTGGAGTCAA GATCTCAGGG TGCAGGAGGT GCGGCGGCTG 
CTCCAGAGCT CCAGACCCGT CCGTGTTAAT GTGCCACATC TGCCGGAGAT CAGTGACCAC 
GAGTACATTG AGGAGAAGGA GAACAAGCTC CTCCAGCTGT GCCAGCGCAC CATGTCTCTC 
CCAGTGGGAC GAGGGCTTTT TACTCTCTTC TCATACCACC CTGTGCCAAC CGAACCACTG 
CCTGTGCCCA AACTCAACCT GACTGGTACT CTCAGAAACG CACACCCATG CTTTAAGCTC 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANAPC1 ( XM_021480904.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021336579.1 Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa148661
Clone ID Related Accession (Same CDS sequence)	XM_021480904.1
Accession Version	XM_021480904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	anaphase-promoting complex subunit 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGGGAA 
CCTCTCCCGA CTTTGTTCAG CATGCTGCAT CCATTAGATG AAATCGCTCC TGTTGTTTGT 
CTGTTTGAAA CCCGGCTCCA GTATGTTTCA GACAGCGCTC TGAGAGTGGT GTTCACCTGC 
CTCAAACCCT CCATTATCAT GACCTACGAC ACACAGCAGT GCACACACAC CGTCTGGAAT 
CTGCGCAAAG TCACATCTGA GGAGCAGAAC ACTGTGTTAA AGTTTCCAGA TCAGTCTGGA 
CCGGCTCACA CTCCTAATGC GGCTCCCAGT TTCCTCAGCA CACACCTGAG AAACGTGAAC 
CGGCTGGACT CTCCGGCTTC ACCTTTACAC TGCCATGCTC TTCACAACCA AAGCCGAATC 
CCAGCCTCCC CGGCCCTGCA CTCTCGGGTT CACTCGCCCA GCATTTCCAA CATGGCTGCT 
CTCAGTCGTG CTCATTCCCC TGGCATCGCC GTGCCCTCGT TCTCTGGGAA TCAGCGTTTC 
AACATGTCAT GCCACACGCC ATCTTCGCGT GGCCACAGCA TCCTGGCTTC ACCCAACAGC 
ACCCTGAATG ACACCCTGCT GCCTGAGATG GAGCCCATCT TACCAGACCT CTGCATCGAG 
CAGCTGTGGA CGGAAACCAG CGCCATCTCC AGGGAGAAAG GCTGCCAGGC GTCTAAAGTC 
TTCATCACCT CTGATCTGTG TGAAAACCGG TACCTGTGCT TCCTGGTCGA GTCCCATCAG 
CAGCTCAGGT GTGTAAAATT TCTTGAGAGC AATGAAACGT CTCAACTGAT CTTTGCAACG 
GTTTCCACCA TCGCTGCAAA AGATGCCGCT CCTCTAGAGG ACATTCACAC AATGCTGGTG 
CTGGAGGCCA ATGGGAGTCT GGTGTTGTAC ACAGGAGTCA CCAGGGTCAG CAAGGTGTTT 
GTCCCTGGCC TTCTGTCTCC TACATTCAGC GTGCCGAACC AGCCGCCAAT ATTGAGCACA 
CCTCTGGAAA ACATCAGCAC TCCGGCCCGA GCCACAGTTC AGCATATGAG CCGTCTGGAG 
GAGGTTGGCA TGCCGTCTCC TGTGCTGGAG ATGCGCAGCT CGTTGCAGCA CAATGAGTCC 
TCATGGCTGG AAGAGTCTCC ATTTCAGCAA ACTGCTCCTC GTATTCAGAC CCTCCGAGAC 
CCCGTCTGCA ACAGAGTCAC ACTGGAATTG AGTTCCGGTT CCATGTTGAG AATCACCATT 
CCCGAGGTGG CCACTTCAGA GCTGGTCAAA CGCTGCCTGC AGGCCATCCG ATTTATTTTA 
CCCAAGGACA CTGCCATGAA AGTGCTGGTC AAGTGGTACA ACAGCTACAA CGCCCCCGGA 
GGTCCCAGCA TGCACCTGGA GTGGAACCTG TTCGTCACCT GCCTAATGAC AATGATGGGT 
TACAACACAG AGCGGCTGAC GTGGACACGC AACCTTCATT TTGAAGTGCC TCTTTCACCA 
GTAATCGCTG CTAAGAAAGC CCGTCCTGCC GACACTGGGT CAGATGAGGA TTGGCTGTAT 
CTCCGCTGCT CAGACTATCA TTGGCAGGTG TCCATGCGGC CTGCGGTGTC TGTTCAGGGT 
CTGGAGATGC TGTTTCCCCC TGCTGGAGTG GATGATCAGG AGTCTGCTCT GGGTTTGGAG 
TCGTCCGCTC TCCTTTTCCC ACACATCCCG CTGCTCTTTT ATGTCCTGCA TCTGCTGTAT 
GAGGACCTGA AGCTGGACGA GTTGCAGAGG AGTGGTGCCA GGGCTCTGGT TGGCCTGCTG 
CAACAGCTTG CCAGGGACCT GCAGCTGGAG GATTTCATTG ATCTGTACTG GCGTGATTTC 
CCCTCATTCA TCAAAACGTT CAAGGAGGCC TGTGTGATTG ATCAAGCTCA GATGGAGCTG 
ATGACGTGGC CGGTGTTTCT GAATGGAGAA GCTCCGTGTG TGTTCAACTG GATCAGTCGC 
TGTCTGAGAG GACAAGATGT TGCGCCGTTT CCATATCTGC CTGGCATCTG TGAGAGAACC 
AAACTACTGG TGCTGAGTTA TGCCCTGTAC GTGGTGGGAG ATGAAGACGC CTGCTCCACC 
AACATGCTCA AATACCTTTG CAGACTGTCA GCAGGGCAAA AGAAGAATGC AAGTGATCCA 
TTTCTGAGAA GGCAGAGCAG TGTGAAGCCC ATATCTTCAT CCGGATGCCT GGCTGAGAAG 
CTGGTGCTGT GGTTAACCTC CGCAGGATTT ACACTGAAGG ATCTCGAGAC GGTGCCGTTC 
GGTGTGGCTC TGCCAATTAG AGACGCCATC TATCAGTGCA GAGAACACCC CTGCTCTGAT 
TGGTCCGAAG AGGTCTGCGC TCTGATTGGC CGACAGGACC TGACCAGACA AGCTCACAAA 
GTCACTCTAG CCAATCGTAA ATCGACGGTG GGTCTTAACG TGATGCTGGA GCCGCCGGTG 
AGCAGCGAAG CCGATGAGGA AGAGGACGGC ATGACGGATC TTCACCCCGA GGTGACCGCG 
CTCATCTGGA GTCAAGATCT CAGGGTGCAG GAGGTGCGGC GGCTGCTCCA GAGCTCCAGA 
CCCGTCCGTG TTAATGTGCC ACATCTGCCG GAGATCAGTG ACCACGAGTA CATTGAGGAG 
AAGGAGAACA AGCTCCTCCA GCTGTGCCAG CGCACCATGT CTCTCCCAGT GGGACGAGGG 
CTTTTTACTC TCTTCTCATA CCACCCTGTG CCAACCGAAC CACTGCCTGT GCCCAAACTC 
AACCTGACTG GCCGTGCTCC TCCCAGGAAC ACCATGGTGG ACCTGAACAG TGGAAACATC 
GACGTACCAC CCAACATGAC CAGCTGGCCC AGCTTTCACA ACGGCGTGGC GGCAGGCCTG 
AAAATCGCTC CTGCTTCTCA AGTTGAGTCC GCTTGGATCG CCTACAACAA ACCCAAAAGT 
GCCGAACTGG CCAATGAGTA TGCTGGTTTC CTGATGGCAC TGGGCCTCAA TGGTCACCTC 
ACCAAACTAG CCACTCTCAA CATCCATGAC TACCTCACCA AGGGTCATGA AATGACGAGT 
ATCGGGCTGC TTTTGGGTGT TTCTGCTGCT AAACTGGGCA CCATGGACAT GTCCATCACT 
CGTCTGCTGA GCATCCACAT CCCTGCATTG CTGCCGCCGA CCTCCACCGA GCTGGACGTC 
CCACACAGTG TGCAGGTGGC TGCTGTGGTC GGCATCGGCC TGGTCTATCA GGGCACCGGA 
CACCGGCACA ACGCAGAGGT GCTGCTGGCA GAGATCGGTC GTCCGCCGGG TCCAGAGATG 
GAATACTGCA CGGACAGAGA GTCATATTCT CTAGCTGCAG GACTGGCACT CGGCATGGTC 
TGCCTCGGGC ACGGCAGTAA CCTGATCGGG ATGAGTGACC TGAACGTGCC CGAGCAGCTC 
TATCAGTACA TGGTGGGAGG TCATCGCCGC ACTCATGCTG GCATCAACAG AGAGAAACAC 
AAATCTCCCA GCTATCAGAT CAAGGAAGGT GACACCATCA ATGTAGACGT GACTTGTCCT 
GGGGCTACAC TTGCTCTGGC CATGATTTAC CTCAAAACAA ATAATAGGTC CGTTGCTGAT 
TGGCTGAAAG CCCCAGACAC CATGTTCCTG CTGGACTTCA TCAAGCCTGA GTTCCTGCTG 
CTGAGGACGC TGGCCAGATG CCTTATCATG TGGGACGAGA TCTTTCCCAA TGCCGACTGG 
GTGACCAGCA ATGTTCCTCA GATCATCCAA GAAAACATCG GAAACCAGGA CCAGACGCCC 
GTCTCGGAGG ACCTCAATAT GGAGACTTTG GTTCAGGCTC ATGACTATAT AATCGCCGGA 
GGCTGTTTGG CTGTGGGATT TCGGTTTGCT GGCTCTGCTA ATTCAGACGC CTTTGACTGC 
TTATACAAAT TTGCCAAAAA CTTCATGCAG TGTTTGACCA CCTCTACTGC TACAGTGACA 
GGCCAGTATA ACCTGCAGAC GTGTTTGAGC ATGGTTCTTC TGGCAGTTTC TATGGTCATG 
TCCGGCTCAG GGAACCTGAA AGTGCTGCAG TTGTGCCGCT TTCTCCACAA ACGCACCTTT 
GGAGAGATGA ACTATGGGTT CCACATGGCT CATCACATGG CCCTGGGTCT GCTCTTCCTG 
GGCGGAGGGA GATACACCCT CAGCACCTCC AACTCGGCCA TCGCCGCGCT GCTCTGTGCA 
CTTTATCCTC ATTTTCCAGC TCACAGCACA GACAACAGGT ATCACCTCCA GGCGTTGCGT 
CATCTGGCTG TCCTTGCGGC AGAACCTCGT CTGCTGGTGC CTGTGGATGT GGACTCTCTC 
AAACCCTGCT ACGCTCTGCT GGATGTCACC TACAAGGAGA CGCAGTGGTA CGAAGAGACC 
ACCGTGGAGT TGATGGCTCC CACAATGCTG CCCGAACTCC ACCTGCTGAA GCGGATTAAG 
GTGAAAGGTC CACGATACTG GGAGCTGAAC ATCGACATCA GTAAAGGAAC CCAGCATCTG 
CAATCCATCC TGTCCAGAGA CGGTGTCCTG TATGTAAAGT TGCGGGCCGG TCAGCTTCCC 
TACAAGGACG ATCCTCAAGG CTGGAAGAGT TTACTGGCCC CGAACCTCAA CAACAGAAGC 
GCAGAAGTTC GCACGTTTAA GCCCGAGGCC ATCTCAACCT TCACCTCAGA CGCCGCTTTG 
GTCTCCTTCG CTGAGCATTT CTGCAGAGCC TCGGAAAACA TGAAGCATAG GCAGGAGACT 
GTGGCTTTAC TGTCCGCCAT ACTGTACGAG TGCGTGACGC AAGAAAACCC AGAAATGCTG 
CCAACGTACA TCTCCATTGA GCAGGCGGTG CGGACTCTGG AGCGGGCCGA TCTGAGCGAG 
ACATTCAGCC TGTGGCAGCT GAAGCTGGTG CTGGAGTTGT GTGAGAGTCG ATTGGCTCGA 
GCTCTGCTGC TCAGCTCGGA GTTTCTGCCT ATCATGAAGA GCTCAGCAGA TCGAGCTCTG 
GATCACTGGC TCTGTGACAA TAGTAATGTG CTGAAGGAGT ACCTAAGACG ACAGACGATG 
AACGGCGACA CTGATGTCAA CATGCTGGCC TGCTTTCTGG TCTATCATTC AATCCCCAGC 
CTCAAAAACA TGCTGGCGTC CGACCTGGAA GGATGTTCCA GTTTTCCAGA GCTGCTCCTC 
AAGAGCAGTC AGTTGGGTGT TCCCATTCGA GCTCTTCTAC GATTGAGTCC TTTACTCCTG 
CAAAATCACG ACTCTGGCGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336579.1
CDS	228..6011
Translation	MSHVYEERATMIAAGELQSFVPFGREHCKNHPNAVNLQLRQLQPASELWSSDGAAGLVGSLQEVTLQERDKERWQLRKGTAPDDVEYDEELYVAGNMVIWSRGSKNQASCVYKAFTVDSPVQQALWCNFTVPQAKKDVKDVVEQTVCIVQSTCVNVHSMSGKDFISPLPFQVSALWPTKFGLLLERKNITVDGHMSPIREPLPTLFSMLHPLDEIAPVVCLFETRLQYVSDSALRVVFTCLKPSIIMTYDTQQCTHTVWNLRKVTSEEQNTVLKFPDQSGPAHTPNAAPSFLSTHLRNVNRLDSPASPLHCHALHNQSRIPASPALHSRVHSPSISNMAALSRAHSPGIAVPSFSGNQRFNMSCHTPSSRGHSILASPNSTLNDTLLPEMEPILPDLCIEQLWTETSAISREKGCQASKVFITSDLCENRYLCFLVESHQQLRCVKFLESNETSQLIFATVSTIAAKDAAPLEDIHTMLVLEANGSLVLYTGVTRVSKVFVPGLLSPTFSVPNQPPILSTPLENISTPARATVQHMSRLEEVGMPSPVLEMRSSLQHNESSWLEESPFQQTAPRIQTLRDPVCNRVTLELSSGSMLRITIPEVATSELVKRCLQAIRFILPKDTAMKVLVKWYNSYNAPGGPSMHLEWNLFVTCLMTMMGYNTERLTWTRNLHFEVPLSPVIAAKKARPADTGSDEDWLYLRCSDYHWQVSMRPAVSVQGLEMLFPPAGVDDQESALGLESSALLFPHIPLLFYVLHLLYEDLKLDELQRSGARALVGLLQQLARDLQLEDFIDLYWRDFPSFIKTFKEACVIDQAQMELMTWPVFLNGEAPCVFNWISRCLRGQDVAPFPYLPGICERTKLLVLSYALYVVGDEDACSTNMLKYLCRLSAGQKKNASDPFLRRQSSVKPISSSGCLAEKLVLWLTSAGFTLKDLETVPFGVALPIRDAIYQCREHPCSDWSEEVCALIGRQDLTRQAHKVTLANRKSTVGLNVMLEPPVSSEADEEEDGMTDLHPEVTALIWSQDLRVQEVRRLLQSSRPVRVNVPHLPEISDHEYIEEKENKLLQLCQRTMSLPVGRGLFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTMVDLNSGNIDVPPNMTSWPSFHNGVAAGLKIAPASQVESAWIAYNKPKSAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHSVQVAAVVGIGLVYQGTGHRHNAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRTHAGINREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSVADWLKAPDTMFLLDFIKPEFLLLRTLARCLIMWDEIFPNADWVTSNVPQIIQENIGNQDQTPVSEDLNMETLVQAHDYIIAGGCLAVGFRFAGSANSDAFDCLYKFAKNFMQCLTTSTATVTGQYNLQTCLSMVLLAVSMVMSGSGNLKVLQLCRFLHKRTFGEMNYGFHMAHHMALGLLFLGGGRYTLSTSNSAIAALLCALYPHFPAHSTDNRYHLQALRHLAVLAAEPRLLVPVDVDSLKPCYALLDVTYKETQWYEETTVELMAPTMLPELHLLKRIKVKGPRYWELNIDISKGTQHLQSILSRDGVLYVKLRAGQLPYKDDPQGWKSLLAPNLNNRSAEVRTFKPEAISTFTSDAALVSFAEHFCRASENMKHRQETVALLSAILYECVTQENPEMLPTYISIEQAVRTLERADLSETFSLWQLKLVLELCESRLARALLLSSEFLPIMKSSADRALDHWLCDNSNVLKEYLRRQTMNGDTDVNMLACFLVYHSIPSLKNMLASDLEGCSSFPELLLKSSQLGVPIRALLRLSPLLLQNHDSGV

Target ORF information:

RefSeq Version	XM_021480904.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480904.1

ORF Insert Sequence:

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGGGAA 
CCTCTCCCGA CTTTGTTCAG CATGCTGCAT CCATTAGATG AAATCGCTCC TGTTGTTTGT 
CTGTTTGAAA CCCGGCTCCA GTATGTTTCA GACAGCGCTC TGAGAGTGGT GTTCACCTGC 
CTCAAACCCT CCATTATCAT GACCTACGAC ACACAGCAGT GCACACACAC CGTCTGGAAT 
CTGCGCAAAG TCACATCTGA GGAGCAGAAC ACTGTGTTAA AGTTTCCAGA TCAGTCTGGA 
CCGGCTCACA CTCCTAATGC GGCTCCCAGT TTCCTCAGCA CACACCTGAG AAACGTGAAC 
CGGCTGGACT CTCCGGCTTC ACCTTTACAC TGCCATGCTC TTCACAACCA AAGCCGAATC 
CCAGCCTCCC CGGCCCTGCA CTCTCGGGTT CACTCGCCCA GCATTTCCAA CATGGCTGCT 
CTCAGTCGTG CTCATTCCCC TGGCATCGCC GTGCCCTCGT TCTCTGGGAA TCAGCGTTTC 
AACATGTCAT GCCACACGCC ATCTTCGCGT GGCCACAGCA TCCTGGCTTC ACCCAACAGC 
ACCCTGAATG ACACCCTGCT GCCTGAGATG GAGCCCATCT TACCAGACCT CTGCATCGAG 
CAGCTGTGGA CGGAAACCAG CGCCATCTCC AGGGAGAAAG GCTGCCAGGC GTCTAAAGTC 
TTCATCACCT CTGATCTGTG TGAAAACCGG TACCTGTGCT TCCTGGTCGA GTCCCATCAG 
CAGCTCAGGT GTGTAAAATT TCTTGAGAGC AATGAAACGT CTCAACTGAT CTTTGCAACG 
GTTTCCACCA TCGCTGCAAA AGATGCCGCT CCTCTAGAGG ACATTCACAC AATGCTGGTG 
CTGGAGGCCA ATGGGAGTCT GGTGTTGTAC ACAGGAGTCA CCAGGGTCAG CAAGGTGTTT 
GTCCCTGGCC TTCTGTCTCC TACATTCAGC GTGCCGAACC AGCCGCCAAT ATTGAGCACA 
CCTCTGGAAA ACATCAGCAC TCCGGCCCGA GCCACAGTTC AGCATATGAG CCGTCTGGAG 
GAGGTTGGCA TGCCGTCTCC TGTGCTGGAG ATGCGCAGCT CGTTGCAGCA CAATGAGTCC 
TCATGGCTGG AAGAGTCTCC ATTTCAGCAA ACTGCTCCTC GTATTCAGAC CCTCCGAGAC 
CCCGTCTGCA ACAGAGTCAC ACTGGAATTG AGTTCCGGTT CCATGTTGAG AATCACCATT 
CCCGAGGTGG CCACTTCAGA GCTGGTCAAA CGCTGCCTGC AGGCCATCCG ATTTATTTTA 
CCCAAGGACA CTGCCATGAA AGTGCTGGTC AAGTGGTACA ACAGCTACAA CGCCCCCGGA 
GGTCCCAGCA TGCACCTGGA GTGGAACCTG TTCGTCACCT GCCTAATGAC AATGATGGGT 
TACAACACAG AGCGGCTGAC GTGGACACGC AACCTTCATT TTGAAGTGCC TCTTTCACCA 
GTAATCGCTG CTAAGAAAGC CCGTCCTGCC GACACTGGGT CAGATGAGGA TTGGCTGTAT 
CTCCGCTGCT CAGACTATCA TTGGCAGGTG TCCATGCGGC CTGCGGTGTC TGTTCAGGGT 
CTGGAGATGC TGTTTCCCCC TGCTGGAGTG GATGATCAGG AGTCTGCTCT GGGTTTGGAG 
TCGTCCGCTC TCCTTTTCCC ACACATCCCG CTGCTCTTTT ATGTCCTGCA TCTGCTGTAT 
GAGGACCTGA AGCTGGACGA GTTGCAGAGG AGTGGTGCCA GGGCTCTGGT TGGCCTGCTG 
CAACAGCTTG CCAGGGACCT GCAGCTGGAG GATTTCATTG ATCTGTACTG GCGTGATTTC 
CCCTCATTCA TCAAAACGTT CAAGGAGGCC TGTGTGATTG ATCAAGCTCA GATGGAGCTG 
ATGACGTGGC CGGTGTTTCT GAATGGAGAA GCTCCGTGTG TGTTCAACTG GATCAGTCGC 
TGTCTGAGAG GACAAGATGT TGCGCCGTTT CCATATCTGC CTGGCATCTG TGAGAGAACC 
AAACTACTGG TGCTGAGTTA TGCCCTGTAC GTGGTGGGAG ATGAAGACGC CTGCTCCACC 
AACATGCTCA AATACCTTTG CAGACTGTCA GCAGGGCAAA AGAAGAATGC AAGTGATCCA 
TTTCTGAGAA GGCAGAGCAG TGTGAAGCCC ATATCTTCAT CCGGATGCCT GGCTGAGAAG 
CTGGTGCTGT GGTTAACCTC CGCAGGATTT ACACTGAAGG ATCTCGAGAC GGTGCCGTTC 
GGTGTGGCTC TGCCAATTAG AGACGCCATC TATCAGTGCA GAGAACACCC CTGCTCTGAT 
TGGTCCGAAG AGGTCTGCGC TCTGATTGGC CGACAGGACC TGACCAGACA AGCTCACAAA 
GTCACTCTAG CCAATCGTAA ATCGACGGTG GGTCTTAACG TGATGCTGGA GCCGCCGGTG 
AGCAGCGAAG CCGATGAGGA AGAGGACGGC ATGACGGATC TTCACCCCGA GGTGACCGCG 
CTCATCTGGA GTCAAGATCT CAGGGTGCAG GAGGTGCGGC GGCTGCTCCA GAGCTCCAGA 
CCCGTCCGTG TTAATGTGCC ACATCTGCCG GAGATCAGTG ACCACGAGTA CATTGAGGAG 
AAGGAGAACA AGCTCCTCCA GCTGTGCCAG CGCACCATGT CTCTCCCAGT GGGACGAGGG 
CTTTTTACTC TCTTCTCATA CCACCCTGTG CCAACCGAAC CACTGCCTGT GCCCAAACTC 
AACCTGACTG GCCGTGCTCC TCCCAGGAAC ACCATGGTGG ACCTGAACAG TGGAAACATC 
GACGTACCAC CCAACATGAC CAGCTGGCCC AGCTTTCACA ACGGCGTGGC GGCAGGCCTG 
AAAATCGCTC CTGCTTCTCA AGTTGAGTCC GCTTGGATCG CCTACAACAA ACCCAAAAGT 
GCCGAACTGG CCAATGAGTA TGCTGGTTTC CTGATGGCAC TGGGCCTCAA TGGTCACCTC 
ACCAAACTAG CCACTCTCAA CATCCATGAC TACCTCACCA AGGGTCATGA AATGACGAGT 
ATCGGGCTGC TTTTGGGTGT TTCTGCTGCT AAACTGGGCA CCATGGACAT GTCCATCACT 
CGTCTGCTGA GCATCCACAT CCCTGCATTG CTGCCGCCGA CCTCCACCGA GCTGGACGTC 
CCACACAGTG TGCAGGTGGC TGCTGTGGTC GGCATCGGCC TGGTCTATCA GGGCACCGGA 
CACCGGCACA ACGCAGAGGT GCTGCTGGCA GAGATCGGTC GTCCGCCGGG TCCAGAGATG 
GAATACTGCA CGGACAGAGA GTCATATTCT CTAGCTGCAG GACTGGCACT CGGCATGGTC 
TGCCTCGGGC ACGGCAGTAA CCTGATCGGG ATGAGTGACC TGAACGTGCC CGAGCAGCTC 
TATCAGTACA TGGTGGGAGG TCATCGCCGC ACTCATGCTG GCATCAACAG AGAGAAACAC 
AAATCTCCCA GCTATCAGAT CAAGGAAGGT GACACCATCA ATGTAGACGT GACTTGTCCT 
GGGGCTACAC TTGCTCTGGC CATGATTTAC CTCAAAACAA ATAATAGGTC CGTTGCTGAT 
TGGCTGAAAG CCCCAGACAC CATGTTCCTG CTGGACTTCA TCAAGCCTGA GTTCCTGCTG 
CTGAGGACGC TGGCCAGATG CCTTATCATG TGGGACGAGA TCTTTCCCAA TGCCGACTGG 
GTGACCAGCA ATGTTCCTCA GATCATCCAA GAAAACATCG GAAACCAGGA CCAGACGCCC 
GTCTCGGAGG ACCTCAATAT GGAGACTTTG GTTCAGGCTC ATGACTATAT AATCGCCGGA 
GGCTGTTTGG CTGTGGGATT TCGGTTTGCT GGCTCTGCTA ATTCAGACGC CTTTGACTGC 
TTATACAAAT TTGCCAAAAA CTTCATGCAG TGTTTGACCA CCTCTACTGC TACAGTGACA 
GGCCAGTATA ACCTGCAGAC GTGTTTGAGC ATGGTTCTTC TGGCAGTTTC TATGGTCATG 
TCCGGCTCAG GGAACCTGAA AGTGCTGCAG TTGTGCCGCT TTCTCCACAA ACGCACCTTT 
GGAGAGATGA ACTATGGGTT CCACATGGCT CATCACATGG CCCTGGGTCT GCTCTTCCTG 
GGCGGAGGGA GATACACCCT CAGCACCTCC AACTCGGCCA TCGCCGCGCT GCTCTGTGCA 
CTTTATCCTC ATTTTCCAGC TCACAGCACA GACAACAGGT ATCACCTCCA GGCGTTGCGT 
CATCTGGCTG TCCTTGCGGC AGAACCTCGT CTGCTGGTGC CTGTGGATGT GGACTCTCTC 
AAACCCTGCT ACGCTCTGCT GGATGTCACC TACAAGGAGA CGCAGTGGTA CGAAGAGACC 
ACCGTGGAGT TGATGGCTCC CACAATGCTG CCCGAACTCC ACCTGCTGAA GCGGATTAAG 
GTGAAAGGTC CACGATACTG GGAGCTGAAC ATCGACATCA GTAAAGGAAC CCAGCATCTG 
CAATCCATCC TGTCCAGAGA CGGTGTCCTG TATGTAAAGT TGCGGGCCGG TCAGCTTCCC 
TACAAGGACG ATCCTCAAGG CTGGAAGAGT TTACTGGCCC CGAACCTCAA CAACAGAAGC 
GCAGAAGTTC GCACGTTTAA GCCCGAGGCC ATCTCAACCT TCACCTCAGA CGCCGCTTTG 
GTCTCCTTCG CTGAGCATTT CTGCAGAGCC TCGGAAAACA TGAAGCATAG GCAGGAGACT 
GTGGCTTTAC TGTCCGCCAT ACTGTACGAG TGCGTGACGC AAGAAAACCC AGAAATGCTG 
CCAACGTACA TCTCCATTGA GCAGGCGGTG CGGACTCTGG AGCGGGCCGA TCTGAGCGAG 
ACATTCAGCC TGTGGCAGCT GAAGCTGGTG CTGGAGTTGT GTGAGAGTCG ATTGGCTCGA 
GCTCTGCTGC TCAGCTCGGA GTTTCTGCCT ATCATGAAGA GCTCAGCAGA TCGAGCTCTG 
GATCACTGGC TCTGTGACAA TAGTAATGTG CTGAAGGAGT ACCTAAGACG ACAGACGATG 
AACGGCGACA CTGATGTCAA CATGCTGGCC TGCTTTCTGG TCTATCATTC AATCCCCAGC 
CTCAAAAACA TGCTGGCGTC CGACCTGGAA GGATGTTCCA GTTTTCCAGA GCTGCTCCTC 
AAGAGCAGTC AGTTGGGTGT TCCCATTCGA GCTCTTCTAC GATTGAGTCC TTTACTCCTG 
CAAAATCACG ACTCTGGCGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANAPC1 ( XM_005157066.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157123.1 Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X1, mRNA.

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CloneID	ODa19121
Clone ID Related Accession (Same CDS sequence)	XM_005157066.3 , XM_005157066.2
Accession Version	XM_005157066.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	anaphase-promoting complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157066.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGCAGG 
GAACCTCTCC CGACTTTGTT CAGCATGCTG CATCCATTAG ATGAAATCGC TCCTGTTGTT 
TGTAAGTCCA CCGGTCTGTT TGAAACCCGG CTCCAGTATG TTTCAGACAG CGCTCTGAGA 
GTGGTGTTCA CCTGCCTCAA ACCCTCCATT ATCATGACCT ACGACACACA GCAGTGCACA 
CACACCGTCT GGAATCTGCG CAAAGTCACA TCTGAGGAGC AGAACACTGT GTTAAAGTTT 
CCAGATCAGT CTGGACCGGC TCACACTCCT AATGCGGCTC CCAGTTTCCT CAGCACACAC 
CTGAGAAACG TGAACCGGCT GGACTCTCCG GCTTCACCTT TACACTGCCA TGCTCTTCAC 
AACCAAAGCC GAATCCCAGC CTCCCCGGCC CTGCACTCTC GGGTTCACTC GCCCAGCATT 
TCCAACATGG CTGCTCTCAG TCGTGCTCAT TCCCCTGGCA TCGCCGTGCC CTCGTTCTCT 
GGGAATCAGC GTTTCAACAT GTCATGCCAC ACGCCATCTT CGCGTGGCCA CAGCATCCTG 
GCTTCACCCA ACAGCACCCT GAATGACACC CTGCTGCCTG AGATGGAGCC CATCTTACCA 
GACCTCTGCA TCGAGCAGCT GTGGACGGAA ACCAGCGCCA TCTCCAGGGA GAAAGGCTGC 
CAGGCGTCTA AAGTCTTCAT CACCTCTGAT CTGTGTGAAA ACCGGTACCT GTGCTTCCTG 
GTCGAGTCCC ATCAGCAGCT CAGGTGTGTA AAATTTCTTG AGAGCAATGA AACGTCTCAA 
CTGATCTTTG CAACGGTTTC CACCATCGCT GCAAAAGATG CCGCTCCTCT AGAGGACATT 
CACACAATGC TGGTGCTGGA GGCCAATGGG AGTCTGGTGT TGTACACAGG AGTCACCAGG 
GTCAGCAAGG TGTTTGTCCC TGGCCTTCTG TCTCCTACAT TCAGCGTGCC GAACCAGCCG 
CCAATATTGA GCACACCTCT GGAAAACATC AGCACTCCGG CCCGAGCCAC AGTTCAGCAT 
ATGAGCCGTC TGGAGGAGGT TGGCATGCCG TCTCCTGTGC TGGAGATGCG CAGCTCGTTG 
CAGCACAATG AGTCCTCATG GCTGGAAGAG TCTCCATTTC AGCAAACTGC TCCTCGTATT 
CAGACCCTCC GAGACCCCGT CTGCAACAGA GTCACACTGG AATTGAGTTC CGGTTCCATG 
TTGAGAATCA CCATTCCCGA GGTGGCCACT TCAGAGCTGG TCAAACGCTG CCTGCAGGCC 
ATCCGATTTA TTTTACCCAA GGACACTGCC ATGAAAGTGC TGGTCAAGTG GTACAACAGC 
TACAACGCCC CCGGAGGTCC CAGCATGCAC CTGGAGTGGA ACCTGTTCGT CACCTGCCTA 
ATGACAATGA TGGGTTACAA CACAGAGCGG CTGACGTGGA CACGCAACCT TCATTTTGAA 
GTGCCTCTTT CACCAGTAAT CGCTGCTAAG AAAGCCCGTC CTGCCGACAC TGGGTCAGAT 
GAGGATTGGC TGTATCTCCG CTGCTCAGAC TATCATTGGC AGGTGTCCAT GCGGCCTGCG 
GTGTCTGTTC AGGGTCTGGA GATGCTGTTT CCCCCTGCTG GAGTGGATGA TCAGGAGTCT 
GCTCTGGGTT TGGAGTCGTC CGCTCTCCTT TTCCCACACA TCCCGCTGCT CTTTTATGTC 
CTGCATCTGC TGTATGAGGA CCTGAAGCTG GACGAGTTGC AGAGGAGTGG TGCCAGGGCT 
CTGGTTGGCC TGCTGCAACA GCTTGCCAGG GACCTGCAGC TGGAGGATTT CATTGATCTG 
TACTGGCGTG ATTTCCCCTC ATTCATCAAA ACGTTCAAGG AGGCCTGTGT GATTGATCAA 
GCTCAGATGG AGCTGATGAC GTGGCCGGTG TTTCTGAATG GAGAAGCTCC GTGTGTGTTC 
AACTGGATCA GTCGCTGTCT GAGAGGACAA GATGTTGCGC CGTTTCCATA TCTGCCTGGC 
ATCTGTGAGA GAACCAAACT ACTGGTGCTG AGTTATGCCC TGTACGTGGT GGGAGATGAA 
GACGCCTGCT CCACCAACAT GCTCAAATAC CTTTGCAGAC TGTCAGCAGG GCAAAAGAAG 
AATGCAAGTG ATCCATTTCT GAGAAGGCAG AGCAGTGTGA AGCCCATATC TTCATCCGGA 
TGCCTGGCTG AGAAGCTGGT GCTGTGGTTA ACCTCCGCAG GATTTACACT GAAGGATCTC 
GAGACGGTGC CGTTCGGTGT GGCTCTGCCA ATTAGAGACG CCATCTATCA GTGCAGAGAA 
CACCCCTGCT CTGATTGGTC CGAAGAGGTC TGCGCTCTGA TTGGCCGACA GGACCTGACC 
AGACAAGCTC ACAAAGTCAC TCTAGCCAAT CGTAAATCGA CGGTGGGTCT TAACGTGATG 
CTGGAGCCGC CGGTGAGCAG CGAAGCCGAT GAGGAAGAGG ACGGCATGAC GGATCTTCAC 
CCCGAGGTGA CCGCGCTCAT CTGGAGTCAA GATCTCAGGG TGCAGGAGGT GCGGCGGCTG 
CTCCAGAGCT CCAGACCCGT CCGTGTTAAT GTGCCACATC TGCCGGAGAT CAGTGACCAC 
GAGTACATTG AGGAGAAGGA GAACAAGCTC CTCCAGCTGT GCCAGCGCAC CATGTCTCTC 
CCAGTGGGAC GAGGGCTTTT TACTCTCTTC TCATACCACC CTGTGCCAAC CGAACCACTG 
CCTGTGCCCA AACTCAACCT GACTGGCCGT GCTCCTCCCA GGAACACCAT GGTGGACCTG 
AACAGTGGAA ACATCGACGT ACCACCCAAC ATGACCAGCT GGCCCAGCTT TCACAACGGC 
GTGGCGGCAG GCCTGAAAAT CGCTCCTGCT TCTCAAGTTG AGTCCGCTTG GATCGCCTAC 
AACAAACCCA AAAGTGCCGA ACTGGCCAAT GAGTATGCTG GTTTCCTGAT GGCACTGGGC 
CTCAATGGTC ACCTCACCAA ACTAGCCACT CTCAACATCC ATGACTACCT CACCAAGGGT 
CATGAAATGA CGAGTATCGG GCTGCTTTTG GGTGTTTCTG CTGCTAAACT GGGCACCATG 
GACATGTCCA TCACTCGTCT GCTGAGCATC CACATCCCTG CATTGCTGCC GCCGACCTCC 
ACCGAGCTGG ACGTCCCACA CAGTGTGCAG GTGGCTGCTG TGGTCGGCAT CGGCCTGGTC 
TATCAGGGCA CCGGACACCG GCACAACGCA GAGGTGCTGC TGGCAGAGAT CGGTCGTCCG 
CCGGGTCCAG AGATGGAATA CTGCACGGAC AGAGAGTCAT ATTCTCTAGC TGCAGGACTG 
GCACTCGGCA TGGTCTGCCT CGGGCACGGC AGTAACCTGA TCGGGATGAG TGACCTGAAC 
GTGCCCGAGC AGCTCTATCA GTACATGGTG GGAGGTCATC GCCGCACTCA TGCTGGCATC 
AACAGAGAGA AACACAAATC TCCCAGCTAT CAGATCAAGG AAGGTGACAC CATCAATGTA 
GACGTGACTT GTCCTGGGGC TACACTTGCT CTGGCCATGA TTTACCTCAA AACAAATAAT 
AGGTCCGTTG CTGATTGGCT GAAAGCCCCA GACACCATGT TCCTGCTGGA CTTCATCAAG 
CCTGAGTTCC TGCTGCTGAG GACGCTGGCC AGATGCCTTA TCATGTGGGA CGAGATCTTT 
CCCAATGCCG ACTGGGTGAC CAGCAATGTT CCTCAGATCA TCCAAGAAAA CATCGGAAAC 
CAGGACCAGA CGCCCGTCTC GGAGGACCTC AATATGGAGA CTTTGGTTCA GGCTCATGAC 
TATATAATCG CCGGAGGCTG TTTGGCTGTG GGATTTCGGT TTGCTGGCTC TGCTAATTCA 
GACGCCTTTG ACTGCTTATA CAAATTTGCC AAAAACTTCA TGCAGTGTTT GACCACCTCT 
ACTGCTACAG TGACAGGCCA GTATAACCTG CAGACGTGTT TGAGCATGGT TCTTCTGGCA 
GTTTCTATGG TCATGTCCGG CTCAGGGAAC CTGAAAGTGC TGCAGTTGTG CCGCTTTCTC 
CACAAACGCA CCTTTGGAGA GATGAACTAT GGGTTCCACA TGGCTCATCA CATGGCCCTG 
GGTCTGCTCT TCCTGGGCGG AGGGAGATAC ACCCTCAGCA CCTCCAACTC GGCCATCGCC 
GCGCTGCTCT GTGCACTTTA TCCTCATTTT CCAGCTCACA GCACAGACAA CAGGTATCAC 
CTCCAGGCGT TGCGTCATCT GGCTGTCCTT GCGGCAGAAC CTCGTCTGCT GGTGCCTGTG 
GATGTGGACT CTCTCAAACC CTGCTACGCT CTGCTGGATG TCACCTACAA GGAGACGCAG 
TGGTACGAAG AGACCACCGT GGAGTTGATG GCTCCCACAA TGCTGCCCGA ACTCCACCTG 
CTGAAGCGGA TTAAGGTGAA AGGTCCACGA TACTGGGAGC TGAACATCGA CATCAGTAAA 
GGAACCCAGC ATCTGCAATC CATCCTGTCC AGAGACGGTG TCCTGTATGT AAAGTTGCGG 
GCCGGTCAGC TTCCCTACAA GGACGATCCT CAAGGCTGGA AGAGTTTACT GGCCCCGAAC 
CTCAACAACA GAAGCGCAGA AGTTCGCACG TTTAAGCCCG AGGCCATCTC AACCTTCACC 
TCAGACGCCG CTTTGGTCTC CTTCGCTGAG CATTTCTGCA GAGCCTCGGA AAACATGAAG 
CATAGGCAGG AGACTGTGGC TTTACTGTCC GCCATACTGT ACGAGTGCGT GACGCAAGAA 
AACCCAGAAA TGCTGCCAAC GTACATCTCC ATTGAGCAGG CGGTGCGGAC TCTGGAGCGG 
GCCGATCTGA GCGAGACATT CAGCCTGTGG CAGCTGAAGC TGGTGCTGGA GTTGTGTGAG 
AGTCGATTGG CTCGAGCTCT GCTGCTCAGC TCGGAGTTTC TGCCTATCAT GAAGAGCTCA 
GCAGATCGAG CTCTGGATCA CTGGCTCTGT GACAATAGTA ATGTGCTGAA GGAGTACCTA 
AGACGACAGA CGATGAACGG CGACACTGAT GTCAACATGC TGGCCTGCTT TCTGGTCTAT 
CATTCAATCC CCAGCCTCAA AAACATGCTG GCGTCCGACC TGGAAGGATG TTCCAGTTTT 
CCAGAGCTGC TCCTCAAGAG CAGTCAGTTG GGTGTTCCCA TTCGAGCTCT TCTACGATTG 
AGTCCTTTAC TCCTGCAAAA TCACGACTCT GGCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157123.1
CDS	228..6026
Translation	MSHVYEERATMIAAGELQSFVPFGREHCKNHPNAVNLQLRQLQPASELWSSDGAAGLVGSLQEVTLQERDKERWQLRKGTAPDDVEYDEELYVAGNMVIWSRGSKNQASCVYKAFTVDSPVQQALWCNFTVPQAKKDVKDVVEQTVCIVQSTCVNVHSMSGKDFISPLPFQVSALWPTKFGLLLERKNITVDGHMSPISREPLPTLFSMLHPLDEIAPVVCKSTGLFETRLQYVSDSALRVVFTCLKPSIIMTYDTQQCTHTVWNLRKVTSEEQNTVLKFPDQSGPAHTPNAAPSFLSTHLRNVNRLDSPASPLHCHALHNQSRIPASPALHSRVHSPSISNMAALSRAHSPGIAVPSFSGNQRFNMSCHTPSSRGHSILASPNSTLNDTLLPEMEPILPDLCIEQLWTETSAISREKGCQASKVFITSDLCENRYLCFLVESHQQLRCVKFLESNETSQLIFATVSTIAAKDAAPLEDIHTMLVLEANGSLVLYTGVTRVSKVFVPGLLSPTFSVPNQPPILSTPLENISTPARATVQHMSRLEEVGMPSPVLEMRSSLQHNESSWLEESPFQQTAPRIQTLRDPVCNRVTLELSSGSMLRITIPEVATSELVKRCLQAIRFILPKDTAMKVLVKWYNSYNAPGGPSMHLEWNLFVTCLMTMMGYNTERLTWTRNLHFEVPLSPVIAAKKARPADTGSDEDWLYLRCSDYHWQVSMRPAVSVQGLEMLFPPAGVDDQESALGLESSALLFPHIPLLFYVLHLLYEDLKLDELQRSGARALVGLLQQLARDLQLEDFIDLYWRDFPSFIKTFKEACVIDQAQMELMTWPVFLNGEAPCVFNWISRCLRGQDVAPFPYLPGICERTKLLVLSYALYVVGDEDACSTNMLKYLCRLSAGQKKNASDPFLRRQSSVKPISSSGCLAEKLVLWLTSAGFTLKDLETVPFGVALPIRDAIYQCREHPCSDWSEEVCALIGRQDLTRQAHKVTLANRKSTVGLNVMLEPPVSSEADEEEDGMTDLHPEVTALIWSQDLRVQEVRRLLQSSRPVRVNVPHLPEISDHEYIEEKENKLLQLCQRTMSLPVGRGLFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTMVDLNSGNIDVPPNMTSWPSFHNGVAAGLKIAPASQVESAWIAYNKPKSAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHSVQVAAVVGIGLVYQGTGHRHNAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRTHAGINREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSVADWLKAPDTMFLLDFIKPEFLLLRTLARCLIMWDEIFPNADWVTSNVPQIIQENIGNQDQTPVSEDLNMETLVQAHDYIIAGGCLAVGFRFAGSANSDAFDCLYKFAKNFMQCLTTSTATVTGQYNLQTCLSMVLLAVSMVMSGSGNLKVLQLCRFLHKRTFGEMNYGFHMAHHMALGLLFLGGGRYTLSTSNSAIAALLCALYPHFPAHSTDNRYHLQALRHLAVLAAEPRLLVPVDVDSLKPCYALLDVTYKETQWYEETTVELMAPTMLPELHLLKRIKVKGPRYWELNIDISKGTQHLQSILSRDGVLYVKLRAGQLPYKDDPQGWKSLLAPNLNNRSAEVRTFKPEAISTFTSDAALVSFAEHFCRASENMKHRQETVALLSAILYECVTQENPEMLPTYISIEQAVRTLERADLSETFSLWQLKLVLELCESRLARALLLSSEFLPIMKSSADRALDHWLCDNSNVLKEYLRRQTMNGDTDVNMLACFLVYHSIPSLKNMLASDLEGCSSFPELLLKSSQLGVPIRALLRLSPLLLQNHDSGV

Target ORF information:

RefSeq Version	XM_005157066.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157066.3

ORF Insert Sequence:

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGCAGG 
GAACCTCTCC CGACTTTGTT CAGCATGCTG CATCCATTAG ATGAAATCGC TCCTGTTGTT 
TGTAAGTCCA CCGGTCTGTT TGAAACCCGG CTCCAGTATG TTTCAGACAG CGCTCTGAGA 
GTGGTGTTCA CCTGCCTCAA ACCCTCCATT ATCATGACCT ACGACACACA GCAGTGCACA 
CACACCGTCT GGAATCTGCG CAAAGTCACA TCTGAGGAGC AGAACACTGT GTTAAAGTTT 
CCAGATCAGT CTGGACCGGC TCACACTCCT AATGCGGCTC CCAGTTTCCT CAGCACACAC 
CTGAGAAACG TGAACCGGCT GGACTCTCCG GCTTCACCTT TACACTGCCA TGCTCTTCAC 
AACCAAAGCC GAATCCCAGC CTCCCCGGCC CTGCACTCTC GGGTTCACTC GCCCAGCATT 
TCCAACATGG CTGCTCTCAG TCGTGCTCAT TCCCCTGGCA TCGCCGTGCC CTCGTTCTCT 
GGGAATCAGC GTTTCAACAT GTCATGCCAC ACGCCATCTT CGCGTGGCCA CAGCATCCTG 
GCTTCACCCA ACAGCACCCT GAATGACACC CTGCTGCCTG AGATGGAGCC CATCTTACCA 
GACCTCTGCA TCGAGCAGCT GTGGACGGAA ACCAGCGCCA TCTCCAGGGA GAAAGGCTGC 
CAGGCGTCTA AAGTCTTCAT CACCTCTGAT CTGTGTGAAA ACCGGTACCT GTGCTTCCTG 
GTCGAGTCCC ATCAGCAGCT CAGGTGTGTA AAATTTCTTG AGAGCAATGA AACGTCTCAA 
CTGATCTTTG CAACGGTTTC CACCATCGCT GCAAAAGATG CCGCTCCTCT AGAGGACATT 
CACACAATGC TGGTGCTGGA GGCCAATGGG AGTCTGGTGT TGTACACAGG AGTCACCAGG 
GTCAGCAAGG TGTTTGTCCC TGGCCTTCTG TCTCCTACAT TCAGCGTGCC GAACCAGCCG 
CCAATATTGA GCACACCTCT GGAAAACATC AGCACTCCGG CCCGAGCCAC AGTTCAGCAT 
ATGAGCCGTC TGGAGGAGGT TGGCATGCCG TCTCCTGTGC TGGAGATGCG CAGCTCGTTG 
CAGCACAATG AGTCCTCATG GCTGGAAGAG TCTCCATTTC AGCAAACTGC TCCTCGTATT 
CAGACCCTCC GAGACCCCGT CTGCAACAGA GTCACACTGG AATTGAGTTC CGGTTCCATG 
TTGAGAATCA CCATTCCCGA GGTGGCCACT TCAGAGCTGG TCAAACGCTG CCTGCAGGCC 
ATCCGATTTA TTTTACCCAA GGACACTGCC ATGAAAGTGC TGGTCAAGTG GTACAACAGC 
TACAACGCCC CCGGAGGTCC CAGCATGCAC CTGGAGTGGA ACCTGTTCGT CACCTGCCTA 
ATGACAATGA TGGGTTACAA CACAGAGCGG CTGACGTGGA CACGCAACCT TCATTTTGAA 
GTGCCTCTTT CACCAGTAAT CGCTGCTAAG AAAGCCCGTC CTGCCGACAC TGGGTCAGAT 
GAGGATTGGC TGTATCTCCG CTGCTCAGAC TATCATTGGC AGGTGTCCAT GCGGCCTGCG 
GTGTCTGTTC AGGGTCTGGA GATGCTGTTT CCCCCTGCTG GAGTGGATGA TCAGGAGTCT 
GCTCTGGGTT TGGAGTCGTC CGCTCTCCTT TTCCCACACA TCCCGCTGCT CTTTTATGTC 
CTGCATCTGC TGTATGAGGA CCTGAAGCTG GACGAGTTGC AGAGGAGTGG TGCCAGGGCT 
CTGGTTGGCC TGCTGCAACA GCTTGCCAGG GACCTGCAGC TGGAGGATTT CATTGATCTG 
TACTGGCGTG ATTTCCCCTC ATTCATCAAA ACGTTCAAGG AGGCCTGTGT GATTGATCAA 
GCTCAGATGG AGCTGATGAC GTGGCCGGTG TTTCTGAATG GAGAAGCTCC GTGTGTGTTC 
AACTGGATCA GTCGCTGTCT GAGAGGACAA GATGTTGCGC CGTTTCCATA TCTGCCTGGC 
ATCTGTGAGA GAACCAAACT ACTGGTGCTG AGTTATGCCC TGTACGTGGT GGGAGATGAA 
GACGCCTGCT CCACCAACAT GCTCAAATAC CTTTGCAGAC TGTCAGCAGG GCAAAAGAAG 
AATGCAAGTG ATCCATTTCT GAGAAGGCAG AGCAGTGTGA AGCCCATATC TTCATCCGGA 
TGCCTGGCTG AGAAGCTGGT GCTGTGGTTA ACCTCCGCAG GATTTACACT GAAGGATCTC 
GAGACGGTGC CGTTCGGTGT GGCTCTGCCA ATTAGAGACG CCATCTATCA GTGCAGAGAA 
CACCCCTGCT CTGATTGGTC CGAAGAGGTC TGCGCTCTGA TTGGCCGACA GGACCTGACC 
AGACAAGCTC ACAAAGTCAC TCTAGCCAAT CGTAAATCGA CGGTGGGTCT TAACGTGATG 
CTGGAGCCGC CGGTGAGCAG CGAAGCCGAT GAGGAAGAGG ACGGCATGAC GGATCTTCAC 
CCCGAGGTGA CCGCGCTCAT CTGGAGTCAA GATCTCAGGG TGCAGGAGGT GCGGCGGCTG 
CTCCAGAGCT CCAGACCCGT CCGTGTTAAT GTGCCACATC TGCCGGAGAT CAGTGACCAC 
GAGTACATTG AGGAGAAGGA GAACAAGCTC CTCCAGCTGT GCCAGCGCAC CATGTCTCTC 
CCAGTGGGAC GAGGGCTTTT TACTCTCTTC TCATACCACC CTGTGCCAAC CGAACCACTG 
CCTGTGCCCA AACTCAACCT GACTGGCCGT GCTCCTCCCA GGAACACCAT GGTGGACCTG 
AACAGTGGAA ACATCGACGT ACCACCCAAC ATGACCAGCT GGCCCAGCTT TCACAACGGC 
GTGGCGGCAG GCCTGAAAAT CGCTCCTGCT TCTCAAGTTG AGTCCGCTTG GATCGCCTAC 
AACAAACCCA AAAGTGCCGA ACTGGCCAAT GAGTATGCTG GTTTCCTGAT GGCACTGGGC 
CTCAATGGTC ACCTCACCAA ACTAGCCACT CTCAACATCC ATGACTACCT CACCAAGGGT 
CATGAAATGA CGAGTATCGG GCTGCTTTTG GGTGTTTCTG CTGCTAAACT GGGCACCATG 
GACATGTCCA TCACTCGTCT GCTGAGCATC CACATCCCTG CATTGCTGCC GCCGACCTCC 
ACCGAGCTGG ACGTCCCACA CAGTGTGCAG GTGGCTGCTG TGGTCGGCAT CGGCCTGGTC 
TATCAGGGCA CCGGACACCG GCACAACGCA GAGGTGCTGC TGGCAGAGAT CGGTCGTCCG 
CCGGGTCCAG AGATGGAATA CTGCACGGAC AGAGAGTCAT ATTCTCTAGC TGCAGGACTG 
GCACTCGGCA TGGTCTGCCT CGGGCACGGC AGTAACCTGA TCGGGATGAG TGACCTGAAC 
GTGCCCGAGC AGCTCTATCA GTACATGGTG GGAGGTCATC GCCGCACTCA TGCTGGCATC 
AACAGAGAGA AACACAAATC TCCCAGCTAT CAGATCAAGG AAGGTGACAC CATCAATGTA 
GACGTGACTT GTCCTGGGGC TACACTTGCT CTGGCCATGA TTTACCTCAA AACAAATAAT 
AGGTCCGTTG CTGATTGGCT GAAAGCCCCA GACACCATGT TCCTGCTGGA CTTCATCAAG 
CCTGAGTTCC TGCTGCTGAG GACGCTGGCC AGATGCCTTA TCATGTGGGA CGAGATCTTT 
CCCAATGCCG ACTGGGTGAC CAGCAATGTT CCTCAGATCA TCCAAGAAAA CATCGGAAAC 
CAGGACCAGA CGCCCGTCTC GGAGGACCTC AATATGGAGA CTTTGGTTCA GGCTCATGAC 
TATATAATCG CCGGAGGCTG TTTGGCTGTG GGATTTCGGT TTGCTGGCTC TGCTAATTCA 
GACGCCTTTG ACTGCTTATA CAAATTTGCC AAAAACTTCA TGCAGTGTTT GACCACCTCT 
ACTGCTACAG TGACAGGCCA GTATAACCTG CAGACGTGTT TGAGCATGGT TCTTCTGGCA 
GTTTCTATGG TCATGTCCGG CTCAGGGAAC CTGAAAGTGC TGCAGTTGTG CCGCTTTCTC 
CACAAACGCA CCTTTGGAGA GATGAACTAT GGGTTCCACA TGGCTCATCA CATGGCCCTG 
GGTCTGCTCT TCCTGGGCGG AGGGAGATAC ACCCTCAGCA CCTCCAACTC GGCCATCGCC 
GCGCTGCTCT GTGCACTTTA TCCTCATTTT CCAGCTCACA GCACAGACAA CAGGTATCAC 
CTCCAGGCGT TGCGTCATCT GGCTGTCCTT GCGGCAGAAC CTCGTCTGCT GGTGCCTGTG 
GATGTGGACT CTCTCAAACC CTGCTACGCT CTGCTGGATG TCACCTACAA GGAGACGCAG 
TGGTACGAAG AGACCACCGT GGAGTTGATG GCTCCCACAA TGCTGCCCGA ACTCCACCTG 
CTGAAGCGGA TTAAGGTGAA AGGTCCACGA TACTGGGAGC TGAACATCGA CATCAGTAAA 
GGAACCCAGC ATCTGCAATC CATCCTGTCC AGAGACGGTG TCCTGTATGT AAAGTTGCGG 
GCCGGTCAGC TTCCCTACAA GGACGATCCT CAAGGCTGGA AGAGTTTACT GGCCCCGAAC 
CTCAACAACA GAAGCGCAGA AGTTCGCACG TTTAAGCCCG AGGCCATCTC AACCTTCACC 
TCAGACGCCG CTTTGGTCTC CTTCGCTGAG CATTTCTGCA GAGCCTCGGA AAACATGAAG 
CATAGGCAGG AGACTGTGGC TTTACTGTCC GCCATACTGT ACGAGTGCGT GACGCAAGAA 
AACCCAGAAA TGCTGCCAAC GTACATCTCC ATTGAGCAGG CGGTGCGGAC TCTGGAGCGG 
GCCGATCTGA GCGAGACATT CAGCCTGTGG CAGCTGAAGC TGGTGCTGGA GTTGTGTGAG 
AGTCGATTGG CTCGAGCTCT GCTGCTCAGC TCGGAGTTTC TGCCTATCAT GAAGAGCTCA 
GCAGATCGAG CTCTGGATCA CTGGCTCTGT GACAATAGTA ATGTGCTGAA GGAGTACCTA 
AGACGACAGA CGATGAACGG CGACACTGAT GTCAACATGC TGGCCTGCTT TCTGGTCTAT 
CATTCAATCC CCAGCCTCAA AAACATGCTG GCGTCCGACC TGGAAGGATG TTCCAGTTTT 
CCAGAGCTGC TCCTCAAGAG CAGTCAGTTG GGTGTTCCCA TTCGAGCTCT TCTACGATTG 
AGTCCTTTAC TCCTGCAAAA TCACGACTCT GGCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANAPC1 ( XM_005157066.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157123.1 Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X1, mRNA.

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CloneID	ODa19121
Clone ID Related Accession (Same CDS sequence)	XM_005157066.3 , XM_005157066.2
Accession Version	XM_005157066.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	anaphase-promoting complex subunit 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157066.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGCAGG 
GAACCTCTCC CGACTTTGTT CAGCATGCTG CATCCATTAG ATGAAATCGC TCCTGTTGTT 
TGTAAGTCCA CCGGTCTGTT TGAAACCCGG CTCCAGTATG TTTCAGACAG CGCTCTGAGA 
GTGGTGTTCA CCTGCCTCAA ACCCTCCATT ATCATGACCT ACGACACACA GCAGTGCACA 
CACACCGTCT GGAATCTGCG CAAAGTCACA TCTGAGGAGC AGAACACTGT GTTAAAGTTT 
CCAGATCAGT CTGGACCGGC TCACACTCCT AATGCGGCTC CCAGTTTCCT CAGCACACAC 
CTGAGAAACG TGAACCGGCT GGACTCTCCG GCTTCACCTT TACACTGCCA TGCTCTTCAC 
AACCAAAGCC GAATCCCAGC CTCCCCGGCC CTGCACTCTC GGGTTCACTC GCCCAGCATT 
TCCAACATGG CTGCTCTCAG TCGTGCTCAT TCCCCTGGCA TCGCCGTGCC CTCGTTCTCT 
GGGAATCAGC GTTTCAACAT GTCATGCCAC ACGCCATCTT CGCGTGGCCA CAGCATCCTG 
GCTTCACCCA ACAGCACCCT GAATGACACC CTGCTGCCTG AGATGGAGCC CATCTTACCA 
GACCTCTGCA TCGAGCAGCT GTGGACGGAA ACCAGCGCCA TCTCCAGGGA GAAAGGCTGC 
CAGGCGTCTA AAGTCTTCAT CACCTCTGAT CTGTGTGAAA ACCGGTACCT GTGCTTCCTG 
GTCGAGTCCC ATCAGCAGCT CAGGTGTGTA AAATTTCTTG AGAGCAATGA AACGTCTCAA 
CTGATCTTTG CAACGGTTTC CACCATCGCT GCAAAAGATG CCGCTCCTCT AGAGGACATT 
CACACAATGC TGGTGCTGGA GGCCAATGGG AGTCTGGTGT TGTACACAGG AGTCACCAGG 
GTCAGCAAGG TGTTTGTCCC TGGCCTTCTG TCTCCTACAT TCAGCGTGCC GAACCAGCCG 
CCAATATTGA GCACACCTCT GGAAAACATC AGCACTCCGG CCCGAGCCAC AGTTCAGCAT 
ATGAGCCGTC TGGAGGAGGT TGGCATGCCG TCTCCTGTGC TGGAGATGCG CAGCTCGTTG 
CAGCACAATG AGTCCTCATG GCTGGAAGAG TCTCCATTTC AGCAAACTGC TCCTCGTATT 
CAGACCCTCC GAGACCCCGT CTGCAACAGA GTCACACTGG AATTGAGTTC CGGTTCCATG 
TTGAGAATCA CCATTCCCGA GGTGGCCACT TCAGAGCTGG TCAAACGCTG CCTGCAGGCC 
ATCCGATTTA TTTTACCCAA GGACACTGCC ATGAAAGTGC TGGTCAAGTG GTACAACAGC 
TACAACGCCC CCGGAGGTCC CAGCATGCAC CTGGAGTGGA ACCTGTTCGT CACCTGCCTA 
ATGACAATGA TGGGTTACAA CACAGAGCGG CTGACGTGGA CACGCAACCT TCATTTTGAA 
GTGCCTCTTT CACCAGTAAT CGCTGCTAAG AAAGCCCGTC CTGCCGACAC TGGGTCAGAT 
GAGGATTGGC TGTATCTCCG CTGCTCAGAC TATCATTGGC AGGTGTCCAT GCGGCCTGCG 
GTGTCTGTTC AGGGTCTGGA GATGCTGTTT CCCCCTGCTG GAGTGGATGA TCAGGAGTCT 
GCTCTGGGTT TGGAGTCGTC CGCTCTCCTT TTCCCACACA TCCCGCTGCT CTTTTATGTC 
CTGCATCTGC TGTATGAGGA CCTGAAGCTG GACGAGTTGC AGAGGAGTGG TGCCAGGGCT 
CTGGTTGGCC TGCTGCAACA GCTTGCCAGG GACCTGCAGC TGGAGGATTT CATTGATCTG 
TACTGGCGTG ATTTCCCCTC ATTCATCAAA ACGTTCAAGG AGGCCTGTGT GATTGATCAA 
GCTCAGATGG AGCTGATGAC GTGGCCGGTG TTTCTGAATG GAGAAGCTCC GTGTGTGTTC 
AACTGGATCA GTCGCTGTCT GAGAGGACAA GATGTTGCGC CGTTTCCATA TCTGCCTGGC 
ATCTGTGAGA GAACCAAACT ACTGGTGCTG AGTTATGCCC TGTACGTGGT GGGAGATGAA 
GACGCCTGCT CCACCAACAT GCTCAAATAC CTTTGCAGAC TGTCAGCAGG GCAAAAGAAG 
AATGCAAGTG ATCCATTTCT GAGAAGGCAG AGCAGTGTGA AGCCCATATC TTCATCCGGA 
TGCCTGGCTG AGAAGCTGGT GCTGTGGTTA ACCTCCGCAG GATTTACACT GAAGGATCTC 
GAGACGGTGC CGTTCGGTGT GGCTCTGCCA ATTAGAGACG CCATCTATCA GTGCAGAGAA 
CACCCCTGCT CTGATTGGTC CGAAGAGGTC TGCGCTCTGA TTGGCCGACA GGACCTGACC 
AGACAAGCTC ACAAAGTCAC TCTAGCCAAT CGTAAATCGA CGGTGGGTCT TAACGTGATG 
CTGGAGCCGC CGGTGAGCAG CGAAGCCGAT GAGGAAGAGG ACGGCATGAC GGATCTTCAC 
CCCGAGGTGA CCGCGCTCAT CTGGAGTCAA GATCTCAGGG TGCAGGAGGT GCGGCGGCTG 
CTCCAGAGCT CCAGACCCGT CCGTGTTAAT GTGCCACATC TGCCGGAGAT CAGTGACCAC 
GAGTACATTG AGGAGAAGGA GAACAAGCTC CTCCAGCTGT GCCAGCGCAC CATGTCTCTC 
CCAGTGGGAC GAGGGCTTTT TACTCTCTTC TCATACCACC CTGTGCCAAC CGAACCACTG 
CCTGTGCCCA AACTCAACCT GACTGGCCGT GCTCCTCCCA GGAACACCAT GGTGGACCTG 
AACAGTGGAA ACATCGACGT ACCACCCAAC ATGACCAGCT GGCCCAGCTT TCACAACGGC 
GTGGCGGCAG GCCTGAAAAT CGCTCCTGCT TCTCAAGTTG AGTCCGCTTG GATCGCCTAC 
AACAAACCCA AAAGTGCCGA ACTGGCCAAT GAGTATGCTG GTTTCCTGAT GGCACTGGGC 
CTCAATGGTC ACCTCACCAA ACTAGCCACT CTCAACATCC ATGACTACCT CACCAAGGGT 
CATGAAATGA CGAGTATCGG GCTGCTTTTG GGTGTTTCTG CTGCTAAACT GGGCACCATG 
GACATGTCCA TCACTCGTCT GCTGAGCATC CACATCCCTG CATTGCTGCC GCCGACCTCC 
ACCGAGCTGG ACGTCCCACA CAGTGTGCAG GTGGCTGCTG TGGTCGGCAT CGGCCTGGTC 
TATCAGGGCA CCGGACACCG GCACAACGCA GAGGTGCTGC TGGCAGAGAT CGGTCGTCCG 
CCGGGTCCAG AGATGGAATA CTGCACGGAC AGAGAGTCAT ATTCTCTAGC TGCAGGACTG 
GCACTCGGCA TGGTCTGCCT CGGGCACGGC AGTAACCTGA TCGGGATGAG TGACCTGAAC 
GTGCCCGAGC AGCTCTATCA GTACATGGTG GGAGGTCATC GCCGCACTCA TGCTGGCATC 
AACAGAGAGA AACACAAATC TCCCAGCTAT CAGATCAAGG AAGGTGACAC CATCAATGTA 
GACGTGACTT GTCCTGGGGC TACACTTGCT CTGGCCATGA TTTACCTCAA AACAAATAAT 
AGGTCCGTTG CTGATTGGCT GAAAGCCCCA GACACCATGT TCCTGCTGGA CTTCATCAAG 
CCTGAGTTCC TGCTGCTGAG GACGCTGGCC AGATGCCTTA TCATGTGGGA CGAGATCTTT 
CCCAATGCCG ACTGGGTGAC CAGCAATGTT CCTCAGATCA TCCAAGAAAA CATCGGAAAC 
CAGGACCAGA CGCCCGTCTC GGAGGACCTC AATATGGAGA CTTTGGTTCA GGCTCATGAC 
TATATAATCG CCGGAGGCTG TTTGGCTGTG GGATTTCGGT TTGCTGGCTC TGCTAATTCA 
GACGCCTTTG ACTGCTTATA CAAATTTGCC AAAAACTTCA TGCAGTGTTT GACCACCTCT 
ACTGCTACAG TGACAGGCCA GTATAACCTG CAGACGTGTT TGAGCATGGT TCTTCTGGCA 
GTTTCTATGG TCATGTCCGG CTCAGGGAAC CTGAAAGTGC TGCAGTTGTG CCGCTTTCTC 
CACAAACGCA CCTTTGGAGA GATGAACTAT GGGTTCCACA TGGCTCATCA CATGGCCCTG 
GGTCTGCTCT TCCTGGGCGG AGGGAGATAC ACCCTCAGCA CCTCCAACTC GGCCATCGCC 
GCGCTGCTCT GTGCACTTTA TCCTCATTTT CCAGCTCACA GCACAGACAA CAGGTATCAC 
CTCCAGGCGT TGCGTCATCT GGCTGTCCTT GCGGCAGAAC CTCGTCTGCT GGTGCCTGTG 
GATGTGGACT CTCTCAAACC CTGCTACGCT CTGCTGGATG TCACCTACAA GGAGACGCAG 
TGGTACGAAG AGACCACCGT GGAGTTGATG GCTCCCACAA TGCTGCCCGA ACTCCACCTG 
CTGAAGCGGA TTAAGGTGAA AGGTCCACGA TACTGGGAGC TGAACATCGA CATCAGTAAA 
GGAACCCAGC ATCTGCAATC CATCCTGTCC AGAGACGGTG TCCTGTATGT AAAGTTGCGG 
GCCGGTCAGC TTCCCTACAA GGACGATCCT CAAGGCTGGA AGAGTTTACT GGCCCCGAAC 
CTCAACAACA GAAGCGCAGA AGTTCGCACG TTTAAGCCCG AGGCCATCTC AACCTTCACC 
TCAGACGCCG CTTTGGTCTC CTTCGCTGAG CATTTCTGCA GAGCCTCGGA AAACATGAAG 
CATAGGCAGG AGACTGTGGC TTTACTGTCC GCCATACTGT ACGAGTGCGT GACGCAAGAA 
AACCCAGAAA TGCTGCCAAC GTACATCTCC ATTGAGCAGG CGGTGCGGAC TCTGGAGCGG 
GCCGATCTGA GCGAGACATT CAGCCTGTGG CAGCTGAAGC TGGTGCTGGA GTTGTGTGAG 
AGTCGATTGG CTCGAGCTCT GCTGCTCAGC TCGGAGTTTC TGCCTATCAT GAAGAGCTCA 
GCAGATCGAG CTCTGGATCA CTGGCTCTGT GACAATAGTA ATGTGCTGAA GGAGTACCTA 
AGACGACAGA CGATGAACGG CGACACTGAT GTCAACATGC TGGCCTGCTT TCTGGTCTAT 
CATTCAATCC CCAGCCTCAA AAACATGCTG GCGTCCGACC TGGAAGGATG TTCCAGTTTT 
CCAGAGCTGC TCCTCAAGAG CAGTCAGTTG GGTGTTCCCA TTCGAGCTCT TCTACGATTG 
AGTCCTTTAC TCCTGCAAAA TCACGACTCT GGCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157123.1
CDS	216..6014
Translation	MSHVYEERATMIAAGELQSFVPFGREHCKNHPNAVNLQLRQLQPASELWSSDGAAGLVGSLQEVTLQERDKERWQLRKGTAPDDVEYDEELYVAGNMVIWSRGSKNQASCVYKAFTVDSPVQQALWCNFTVPQAKKDVKDVVEQTVCIVQSTCVNVHSMSGKDFISPLPFQVSALWPTKFGLLLERKNITVDGHMSPISREPLPTLFSMLHPLDEIAPVVCKSTGLFETRLQYVSDSALRVVFTCLKPSIIMTYDTQQCTHTVWNLRKVTSEEQNTVLKFPDQSGPAHTPNAAPSFLSTHLRNVNRLDSPASPLHCHALHNQSRIPASPALHSRVHSPSISNMAALSRAHSPGIAVPSFSGNQRFNMSCHTPSSRGHSILASPNSTLNDTLLPEMEPILPDLCIEQLWTETSAISREKGCQASKVFITSDLCENRYLCFLVESHQQLRCVKFLESNETSQLIFATVSTIAAKDAAPLEDIHTMLVLEANGSLVLYTGVTRVSKVFVPGLLSPTFSVPNQPPILSTPLENISTPARATVQHMSRLEEVGMPSPVLEMRSSLQHNESSWLEESPFQQTAPRIQTLRDPVCNRVTLELSSGSMLRITIPEVATSELVKRCLQAIRFILPKDTAMKVLVKWYNSYNAPGGPSMHLEWNLFVTCLMTMMGYNTERLTWTRNLHFEVPLSPVIAAKKARPADTGSDEDWLYLRCSDYHWQVSMRPAVSVQGLEMLFPPAGVDDQESALGLESSALLFPHIPLLFYVLHLLYEDLKLDELQRSGARALVGLLQQLARDLQLEDFIDLYWRDFPSFIKTFKEACVIDQAQMELMTWPVFLNGEAPCVFNWISRCLRGQDVAPFPYLPGICERTKLLVLSYALYVVGDEDACSTNMLKYLCRLSAGQKKNASDPFLRRQSSVKPISSSGCLAEKLVLWLTSAGFTLKDLETVPFGVALPIRDAIYQCREHPCSDWSEEVCALIGRQDLTRQAHKVTLANRKSTVGLNVMLEPPVSSEADEEEDGMTDLHPEVTALIWSQDLRVQEVRRLLQSSRPVRVNVPHLPEISDHEYIEEKENKLLQLCQRTMSLPVGRGLFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTMVDLNSGNIDVPPNMTSWPSFHNGVAAGLKIAPASQVESAWIAYNKPKSAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHSVQVAAVVGIGLVYQGTGHRHNAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRTHAGINREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSVADWLKAPDTMFLLDFIKPEFLLLRTLARCLIMWDEIFPNADWVTSNVPQIIQENIGNQDQTPVSEDLNMETLVQAHDYIIAGGCLAVGFRFAGSANSDAFDCLYKFAKNFMQCLTTSTATVTGQYNLQTCLSMVLLAVSMVMSGSGNLKVLQLCRFLHKRTFGEMNYGFHMAHHMALGLLFLGGGRYTLSTSNSAIAALLCALYPHFPAHSTDNRYHLQALRHLAVLAAEPRLLVPVDVDSLKPCYALLDVTYKETQWYEETTVELMAPTMLPELHLLKRIKVKGPRYWELNIDISKGTQHLQSILSRDGVLYVKLRAGQLPYKDDPQGWKSLLAPNLNNRSAEVRTFKPEAISTFTSDAALVSFAEHFCRASENMKHRQETVALLSAILYECVTQENPEMLPTYISIEQAVRTLERADLSETFSLWQLKLVLELCESRLARALLLSSEFLPIMKSSADRALDHWLCDNSNVLKEYLRRQTMNGDTDVNMLACFLVYHSIPSLKNMLASDLEGCSSFPELLLKSSQLGVPIRALLRLSPLLLQNHDSGV

Target ORF information:

RefSeq Version	XM_005157066.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157066.2

ORF Insert Sequence:

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGCAGG 
GAACCTCTCC CGACTTTGTT CAGCATGCTG CATCCATTAG ATGAAATCGC TCCTGTTGTT 
TGTAAGTCCA CCGGTCTGTT TGAAACCCGG CTCCAGTATG TTTCAGACAG CGCTCTGAGA 
GTGGTGTTCA CCTGCCTCAA ACCCTCCATT ATCATGACCT ACGACACACA GCAGTGCACA 
CACACCGTCT GGAATCTGCG CAAAGTCACA TCTGAGGAGC AGAACACTGT GTTAAAGTTT 
CCAGATCAGT CTGGACCGGC TCACACTCCT AATGCGGCTC CCAGTTTCCT CAGCACACAC 
CTGAGAAACG TGAACCGGCT GGACTCTCCG GCTTCACCTT TACACTGCCA TGCTCTTCAC 
AACCAAAGCC GAATCCCAGC CTCCCCGGCC CTGCACTCTC GGGTTCACTC GCCCAGCATT 
TCCAACATGG CTGCTCTCAG TCGTGCTCAT TCCCCTGGCA TCGCCGTGCC CTCGTTCTCT 
GGGAATCAGC GTTTCAACAT GTCATGCCAC ACGCCATCTT CGCGTGGCCA CAGCATCCTG 
GCTTCACCCA ACAGCACCCT GAATGACACC CTGCTGCCTG AGATGGAGCC CATCTTACCA 
GACCTCTGCA TCGAGCAGCT GTGGACGGAA ACCAGCGCCA TCTCCAGGGA GAAAGGCTGC 
CAGGCGTCTA AAGTCTTCAT CACCTCTGAT CTGTGTGAAA ACCGGTACCT GTGCTTCCTG 
GTCGAGTCCC ATCAGCAGCT CAGGTGTGTA AAATTTCTTG AGAGCAATGA AACGTCTCAA 
CTGATCTTTG CAACGGTTTC CACCATCGCT GCAAAAGATG CCGCTCCTCT AGAGGACATT 
CACACAATGC TGGTGCTGGA GGCCAATGGG AGTCTGGTGT TGTACACAGG AGTCACCAGG 
GTCAGCAAGG TGTTTGTCCC TGGCCTTCTG TCTCCTACAT TCAGCGTGCC GAACCAGCCG 
CCAATATTGA GCACACCTCT GGAAAACATC AGCACTCCGG CCCGAGCCAC AGTTCAGCAT 
ATGAGCCGTC TGGAGGAGGT TGGCATGCCG TCTCCTGTGC TGGAGATGCG CAGCTCGTTG 
CAGCACAATG AGTCCTCATG GCTGGAAGAG TCTCCATTTC AGCAAACTGC TCCTCGTATT 
CAGACCCTCC GAGACCCCGT CTGCAACAGA GTCACACTGG AATTGAGTTC CGGTTCCATG 
TTGAGAATCA CCATTCCCGA GGTGGCCACT TCAGAGCTGG TCAAACGCTG CCTGCAGGCC 
ATCCGATTTA TTTTACCCAA GGACACTGCC ATGAAAGTGC TGGTCAAGTG GTACAACAGC 
TACAACGCCC CCGGAGGTCC CAGCATGCAC CTGGAGTGGA ACCTGTTCGT CACCTGCCTA 
ATGACAATGA TGGGTTACAA CACAGAGCGG CTGACGTGGA CACGCAACCT TCATTTTGAA 
GTGCCTCTTT CACCAGTAAT CGCTGCTAAG AAAGCCCGTC CTGCCGACAC TGGGTCAGAT 
GAGGATTGGC TGTATCTCCG CTGCTCAGAC TATCATTGGC AGGTGTCCAT GCGGCCTGCG 
GTGTCTGTTC AGGGTCTGGA GATGCTGTTT CCCCCTGCTG GAGTGGATGA TCAGGAGTCT 
GCTCTGGGTT TGGAGTCGTC CGCTCTCCTT TTCCCACACA TCCCGCTGCT CTTTTATGTC 
CTGCATCTGC TGTATGAGGA CCTGAAGCTG GACGAGTTGC AGAGGAGTGG TGCCAGGGCT 
CTGGTTGGCC TGCTGCAACA GCTTGCCAGG GACCTGCAGC TGGAGGATTT CATTGATCTG 
TACTGGCGTG ATTTCCCCTC ATTCATCAAA ACGTTCAAGG AGGCCTGTGT GATTGATCAA 
GCTCAGATGG AGCTGATGAC GTGGCCGGTG TTTCTGAATG GAGAAGCTCC GTGTGTGTTC 
AACTGGATCA GTCGCTGTCT GAGAGGACAA GATGTTGCGC CGTTTCCATA TCTGCCTGGC 
ATCTGTGAGA GAACCAAACT ACTGGTGCTG AGTTATGCCC TGTACGTGGT GGGAGATGAA 
GACGCCTGCT CCACCAACAT GCTCAAATAC CTTTGCAGAC TGTCAGCAGG GCAAAAGAAG 
AATGCAAGTG ATCCATTTCT GAGAAGGCAG AGCAGTGTGA AGCCCATATC TTCATCCGGA 
TGCCTGGCTG AGAAGCTGGT GCTGTGGTTA ACCTCCGCAG GATTTACACT GAAGGATCTC 
GAGACGGTGC CGTTCGGTGT GGCTCTGCCA ATTAGAGACG CCATCTATCA GTGCAGAGAA 
CACCCCTGCT CTGATTGGTC CGAAGAGGTC TGCGCTCTGA TTGGCCGACA GGACCTGACC 
AGACAAGCTC ACAAAGTCAC TCTAGCCAAT CGTAAATCGA CGGTGGGTCT TAACGTGATG 
CTGGAGCCGC CGGTGAGCAG CGAAGCCGAT GAGGAAGAGG ACGGCATGAC GGATCTTCAC 
CCCGAGGTGA CCGCGCTCAT CTGGAGTCAA GATCTCAGGG TGCAGGAGGT GCGGCGGCTG 
CTCCAGAGCT CCAGACCCGT CCGTGTTAAT GTGCCACATC TGCCGGAGAT CAGTGACCAC 
GAGTACATTG AGGAGAAGGA GAACAAGCTC CTCCAGCTGT GCCAGCGCAC CATGTCTCTC 
CCAGTGGGAC GAGGGCTTTT TACTCTCTTC TCATACCACC CTGTGCCAAC CGAACCACTG 
CCTGTGCCCA AACTCAACCT GACTGGCCGT GCTCCTCCCA GGAACACCAT GGTGGACCTG 
AACAGTGGAA ACATCGACGT ACCACCCAAC ATGACCAGCT GGCCCAGCTT TCACAACGGC 
GTGGCGGCAG GCCTGAAAAT CGCTCCTGCT TCTCAAGTTG AGTCCGCTTG GATCGCCTAC 
AACAAACCCA AAAGTGCCGA ACTGGCCAAT GAGTATGCTG GTTTCCTGAT GGCACTGGGC 
CTCAATGGTC ACCTCACCAA ACTAGCCACT CTCAACATCC ATGACTACCT CACCAAGGGT 
CATGAAATGA CGAGTATCGG GCTGCTTTTG GGTGTTTCTG CTGCTAAACT GGGCACCATG 
GACATGTCCA TCACTCGTCT GCTGAGCATC CACATCCCTG CATTGCTGCC GCCGACCTCC 
ACCGAGCTGG ACGTCCCACA CAGTGTGCAG GTGGCTGCTG TGGTCGGCAT CGGCCTGGTC 
TATCAGGGCA CCGGACACCG GCACAACGCA GAGGTGCTGC TGGCAGAGAT CGGTCGTCCG 
CCGGGTCCAG AGATGGAATA CTGCACGGAC AGAGAGTCAT ATTCTCTAGC TGCAGGACTG 
GCACTCGGCA TGGTCTGCCT CGGGCACGGC AGTAACCTGA TCGGGATGAG TGACCTGAAC 
GTGCCCGAGC AGCTCTATCA GTACATGGTG GGAGGTCATC GCCGCACTCA TGCTGGCATC 
AACAGAGAGA AACACAAATC TCCCAGCTAT CAGATCAAGG AAGGTGACAC CATCAATGTA 
GACGTGACTT GTCCTGGGGC TACACTTGCT CTGGCCATGA TTTACCTCAA AACAAATAAT 
AGGTCCGTTG CTGATTGGCT GAAAGCCCCA GACACCATGT TCCTGCTGGA CTTCATCAAG 
CCTGAGTTCC TGCTGCTGAG GACGCTGGCC AGATGCCTTA TCATGTGGGA CGAGATCTTT 
CCCAATGCCG ACTGGGTGAC CAGCAATGTT CCTCAGATCA TCCAAGAAAA CATCGGAAAC 
CAGGACCAGA CGCCCGTCTC GGAGGACCTC AATATGGAGA CTTTGGTTCA GGCTCATGAC 
TATATAATCG CCGGAGGCTG TTTGGCTGTG GGATTTCGGT TTGCTGGCTC TGCTAATTCA 
GACGCCTTTG ACTGCTTATA CAAATTTGCC AAAAACTTCA TGCAGTGTTT GACCACCTCT 
ACTGCTACAG TGACAGGCCA GTATAACCTG CAGACGTGTT TGAGCATGGT TCTTCTGGCA 
GTTTCTATGG TCATGTCCGG CTCAGGGAAC CTGAAAGTGC TGCAGTTGTG CCGCTTTCTC 
CACAAACGCA CCTTTGGAGA GATGAACTAT GGGTTCCACA TGGCTCATCA CATGGCCCTG 
GGTCTGCTCT TCCTGGGCGG AGGGAGATAC ACCCTCAGCA CCTCCAACTC GGCCATCGCC 
GCGCTGCTCT GTGCACTTTA TCCTCATTTT CCAGCTCACA GCACAGACAA CAGGTATCAC 
CTCCAGGCGT TGCGTCATCT GGCTGTCCTT GCGGCAGAAC CTCGTCTGCT GGTGCCTGTG 
GATGTGGACT CTCTCAAACC CTGCTACGCT CTGCTGGATG TCACCTACAA GGAGACGCAG 
TGGTACGAAG AGACCACCGT GGAGTTGATG GCTCCCACAA TGCTGCCCGA ACTCCACCTG 
CTGAAGCGGA TTAAGGTGAA AGGTCCACGA TACTGGGAGC TGAACATCGA CATCAGTAAA 
GGAACCCAGC ATCTGCAATC CATCCTGTCC AGAGACGGTG TCCTGTATGT AAAGTTGCGG 
GCCGGTCAGC TTCCCTACAA GGACGATCCT CAAGGCTGGA AGAGTTTACT GGCCCCGAAC 
CTCAACAACA GAAGCGCAGA AGTTCGCACG TTTAAGCCCG AGGCCATCTC AACCTTCACC 
TCAGACGCCG CTTTGGTCTC CTTCGCTGAG CATTTCTGCA GAGCCTCGGA AAACATGAAG 
CATAGGCAGG AGACTGTGGC TTTACTGTCC GCCATACTGT ACGAGTGCGT GACGCAAGAA 
AACCCAGAAA TGCTGCCAAC GTACATCTCC ATTGAGCAGG CGGTGCGGAC TCTGGAGCGG 
GCCGATCTGA GCGAGACATT CAGCCTGTGG CAGCTGAAGC TGGTGCTGGA GTTGTGTGAG 
AGTCGATTGG CTCGAGCTCT GCTGCTCAGC TCGGAGTTTC TGCCTATCAT GAAGAGCTCA 
GCAGATCGAG CTCTGGATCA CTGGCTCTGT GACAATAGTA ATGTGCTGAA GGAGTACCTA 
AGACGACAGA CGATGAACGG CGACACTGAT GTCAACATGC TGGCCTGCTT TCTGGTCTAT 
CATTCAATCC CCAGCCTCAA AAACATGCTG GCGTCCGACC TGGAAGGATG TTCCAGTTTT 
CCAGAGCTGC TCCTCAAGAG CAGTCAGTTG GGTGTTCCCA TTCGAGCTCT TCTACGATTG 
AGTCCTTTAC TCCTGCAAAA TCACGACTCT GGCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANAPC1 ( XM_001921133.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001921168.2 Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X2, mRNA.

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CloneID	ODa19122
Clone ID Related Accession (Same CDS sequence)	XM_001921133.4 , XM_001921133.3
Accession Version	XM_001921133.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	anaphase-promoting complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001921133.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGGGAA 
CCTCTCCCGA CTTTGTTCAG CATGCTGCAT CCATTAGATG AAATCGCTCC TGTTGTTTGT 
AAGTCCACCG GTCTGTTTGA AACCCGGCTC CAGTATGTTT CAGACAGCGC TCTGAGAGTG 
GTGTTCACCT GCCTCAAACC CTCCATTATC ATGACCTACG ACACACAGCA GTGCACACAC 
ACCGTCTGGA ATCTGCGCAA AGTCACATCT GAGGAGCAGA ACACTGTGTT AAAGTTTCCA 
GATCAGTCTG GACCGGCTCA CACTCCTAAT GCGGCTCCCA GTTTCCTCAG CACACACCTG 
AGAAACGTGA ACCGGCTGGA CTCTCCGGCT TCACCTTTAC ACTGCCATGC TCTTCACAAC 
CAAAGCCGAA TCCCAGCCTC CCCGGCCCTG CACTCTCGGG TTCACTCGCC CAGCATTTCC 
AACATGGCTG CTCTCAGTCG TGCTCATTCC CCTGGCATCG CCGTGCCCTC GTTCTCTGGG 
AATCAGCGTT TCAACATGTC ATGCCACACG CCATCTTCGC GTGGCCACAG CATCCTGGCT 
TCACCCAACA GCACCCTGAA TGACACCCTG CTGCCTGAGA TGGAGCCCAT CTTACCAGAC 
CTCTGCATCG AGCAGCTGTG GACGGAAACC AGCGCCATCT CCAGGGAGAA AGGCTGCCAG 
GCGTCTAAAG TCTTCATCAC CTCTGATCTG TGTGAAAACC GGTACCTGTG CTTCCTGGTC 
GAGTCCCATC AGCAGCTCAG GTGTGTAAAA TTTCTTGAGA GCAATGAAAC GTCTCAACTG 
ATCTTTGCAA CGGTTTCCAC CATCGCTGCA AAAGATGCCG CTCCTCTAGA GGACATTCAC 
ACAATGCTGG TGCTGGAGGC CAATGGGAGT CTGGTGTTGT ACACAGGAGT CACCAGGGTC 
AGCAAGGTGT TTGTCCCTGG CCTTCTGTCT CCTACATTCA GCGTGCCGAA CCAGCCGCCA 
ATATTGAGCA CACCTCTGGA AAACATCAGC ACTCCGGCCC GAGCCACAGT TCAGCATATG 
AGCCGTCTGG AGGAGGTTGG CATGCCGTCT CCTGTGCTGG AGATGCGCAG CTCGTTGCAG 
CACAATGAGT CCTCATGGCT GGAAGAGTCT CCATTTCAGC AAACTGCTCC TCGTATTCAG 
ACCCTCCGAG ACCCCGTCTG CAACAGAGTC ACACTGGAAT TGAGTTCCGG TTCCATGTTG 
AGAATCACCA TTCCCGAGGT GGCCACTTCA GAGCTGGTCA AACGCTGCCT GCAGGCCATC 
CGATTTATTT TACCCAAGGA CACTGCCATG AAAGTGCTGG TCAAGTGGTA CAACAGCTAC 
AACGCCCCCG GAGGTCCCAG CATGCACCTG GAGTGGAACC TGTTCGTCAC CTGCCTAATG 
ACAATGATGG GTTACAACAC AGAGCGGCTG ACGTGGACAC GCAACCTTCA TTTTGAAGTG 
CCTCTTTCAC CAGTAATCGC TGCTAAGAAA GCCCGTCCTG CCGACACTGG GTCAGATGAG 
GATTGGCTGT ATCTCCGCTG CTCAGACTAT CATTGGCAGG TGTCCATGCG GCCTGCGGTG 
TCTGTTCAGG GTCTGGAGAT GCTGTTTCCC CCTGCTGGAG TGGATGATCA GGAGTCTGCT 
CTGGGTTTGG AGTCGTCCGC TCTCCTTTTC CCACACATCC CGCTGCTCTT TTATGTCCTG 
CATCTGCTGT ATGAGGACCT GAAGCTGGAC GAGTTGCAGA GGAGTGGTGC CAGGGCTCTG 
GTTGGCCTGC TGCAACAGCT TGCCAGGGAC CTGCAGCTGG AGGATTTCAT TGATCTGTAC 
TGGCGTGATT TCCCCTCATT CATCAAAACG TTCAAGGAGG CCTGTGTGAT TGATCAAGCT 
CAGATGGAGC TGATGACGTG GCCGGTGTTT CTGAATGGAG AAGCTCCGTG TGTGTTCAAC 
TGGATCAGTC GCTGTCTGAG AGGACAAGAT GTTGCGCCGT TTCCATATCT GCCTGGCATC 
TGTGAGAGAA CCAAACTACT GGTGCTGAGT TATGCCCTGT ACGTGGTGGG AGATGAAGAC 
GCCTGCTCCA CCAACATGCT CAAATACCTT TGCAGACTGT CAGCAGGGCA AAAGAAGAAT 
GCAAGTGATC CATTTCTGAG AAGGCAGAGC AGTGTGAAGC CCATATCTTC ATCCGGATGC 
CTGGCTGAGA AGCTGGTGCT GTGGTTAACC TCCGCAGGAT TTACACTGAA GGATCTCGAG 
ACGGTGCCGT TCGGTGTGGC TCTGCCAATT AGAGACGCCA TCTATCAGTG CAGAGAACAC 
CCCTGCTCTG ATTGGTCCGA AGAGGTCTGC GCTCTGATTG GCCGACAGGA CCTGACCAGA 
CAAGCTCACA AAGTCACTCT AGCCAATCGT AAATCGACGG TGGGTCTTAA CGTGATGCTG 
GAGCCGCCGG TGAGCAGCGA AGCCGATGAG GAAGAGGACG GCATGACGGA TCTTCACCCC 
GAGGTGACCG CGCTCATCTG GAGTCAAGAT CTCAGGGTGC AGGAGGTGCG GCGGCTGCTC 
CAGAGCTCCA GACCCGTCCG TGTTAATGTG CCACATCTGC CGGAGATCAG TGACCACGAG 
TACATTGAGG AGAAGGAGAA CAAGCTCCTC CAGCTGTGCC AGCGCACCAT GTCTCTCCCA 
GTGGGACGAG GGCTTTTTAC TCTCTTCTCA TACCACCCTG TGCCAACCGA ACCACTGCCT 
GTGCCCAAAC TCAACCTGAC TGGCCGTGCT CCTCCCAGGA ACACCATGGT GGACCTGAAC 
AGTGGAAACA TCGACGTACC ACCCAACATG ACCAGCTGGC CCAGCTTTCA CAACGGCGTG 
GCGGCAGGCC TGAAAATCGC TCCTGCTTCT CAAGTTGAGT CCGCTTGGAT CGCCTACAAC 
AAACCCAAAA GTGCCGAACT GGCCAATGAG TATGCTGGTT TCCTGATGGC ACTGGGCCTC 
AATGGTCACC TCACCAAACT AGCCACTCTC AACATCCATG ACTACCTCAC CAAGGGTCAT 
GAAATGACGA GTATCGGGCT GCTTTTGGGT GTTTCTGCTG CTAAACTGGG CACCATGGAC 
ATGTCCATCA CTCGTCTGCT GAGCATCCAC ATCCCTGCAT TGCTGCCGCC GACCTCCACC 
GAGCTGGACG TCCCACACAG TGTGCAGGTG GCTGCTGTGG TCGGCATCGG CCTGGTCTAT 
CAGGGCACCG GACACCGGCA CAACGCAGAG GTGCTGCTGG CAGAGATCGG TCGTCCGCCG 
GGTCCAGAGA TGGAATACTG CACGGACAGA GAGTCATATT CTCTAGCTGC AGGACTGGCA 
CTCGGCATGG TCTGCCTCGG GCACGGCAGT AACCTGATCG GGATGAGTGA CCTGAACGTG 
CCCGAGCAGC TCTATCAGTA CATGGTGGGA GGTCATCGCC GCACTCATGC TGGCATCAAC 
AGAGAGAAAC ACAAATCTCC CAGCTATCAG ATCAAGGAAG GTGACACCAT CAATGTAGAC 
GTGACTTGTC CTGGGGCTAC ACTTGCTCTG GCCATGATTT ACCTCAAAAC AAATAATAGG 
TCCGTTGCTG ATTGGCTGAA AGCCCCAGAC ACCATGTTCC TGCTGGACTT CATCAAGCCT 
GAGTTCCTGC TGCTGAGGAC GCTGGCCAGA TGCCTTATCA TGTGGGACGA GATCTTTCCC 
AATGCCGACT GGGTGACCAG CAATGTTCCT CAGATCATCC AAGAAAACAT CGGAAACCAG 
GACCAGACGC CCGTCTCGGA GGACCTCAAT ATGGAGACTT TGGTTCAGGC TCATGACTAT 
ATAATCGCCG GAGGCTGTTT GGCTGTGGGA TTTCGGTTTG CTGGCTCTGC TAATTCAGAC 
GCCTTTGACT GCTTATACAA ATTTGCCAAA AACTTCATGC AGTGTTTGAC CACCTCTACT 
GCTACAGTGA CAGGCCAGTA TAACCTGCAG ACGTGTTTGA GCATGGTTCT TCTGGCAGTT 
TCTATGGTCA TGTCCGGCTC AGGGAACCTG AAAGTGCTGC AGTTGTGCCG CTTTCTCCAC 
AAACGCACCT TTGGAGAGAT GAACTATGGG TTCCACATGG CTCATCACAT GGCCCTGGGT 
CTGCTCTTCC TGGGCGGAGG GAGATACACC CTCAGCACCT CCAACTCGGC CATCGCCGCG 
CTGCTCTGTG CACTTTATCC TCATTTTCCA GCTCACAGCA CAGACAACAG GTATCACCTC 
CAGGCGTTGC GTCATCTGGC TGTCCTTGCG GCAGAACCTC GTCTGCTGGT GCCTGTGGAT 
GTGGACTCTC TCAAACCCTG CTACGCTCTG CTGGATGTCA CCTACAAGGA GACGCAGTGG 
TACGAAGAGA CCACCGTGGA GTTGATGGCT CCCACAATGC TGCCCGAACT CCACCTGCTG 
AAGCGGATTA AGGTGAAAGG TCCACGATAC TGGGAGCTGA ACATCGACAT CAGTAAAGGA 
ACCCAGCATC TGCAATCCAT CCTGTCCAGA GACGGTGTCC TGTATGTAAA GTTGCGGGCC 
GGTCAGCTTC CCTACAAGGA CGATCCTCAA GGCTGGAAGA GTTTACTGGC CCCGAACCTC 
AACAACAGAA GCGCAGAAGT TCGCACGTTT AAGCCCGAGG CCATCTCAAC CTTCACCTCA 
GACGCCGCTT TGGTCTCCTT CGCTGAGCAT TTCTGCAGAG CCTCGGAAAA CATGAAGCAT 
AGGCAGGAGA CTGTGGCTTT ACTGTCCGCC ATACTGTACG AGTGCGTGAC GCAAGAAAAC 
CCAGAAATGC TGCCAACGTA CATCTCCATT GAGCAGGCGG TGCGGACTCT GGAGCGGGCC 
GATCTGAGCG AGACATTCAG CCTGTGGCAG CTGAAGCTGG TGCTGGAGTT GTGTGAGAGT 
CGATTGGCTC GAGCTCTGCT GCTCAGCTCG GAGTTTCTGC CTATCATGAA GAGCTCAGCA 
GATCGAGCTC TGGATCACTG GCTCTGTGAC AATAGTAATG TGCTGAAGGA GTACCTAAGA 
CGACAGACGA TGAACGGCGA CACTGATGTC AACATGCTGG CCTGCTTTCT GGTCTATCAT 
TCAATCCCCA GCCTCAAAAA CATGCTGGCG TCCGACCTGG AAGGATGTTC CAGTTTTCCA 
GAGCTGCTCC TCAAGAGCAG TCAGTTGGGT GTTCCCATTC GAGCTCTTCT ACGATTGAGT 
CCTTTACTCC TGCAAAATCA CGACTCTGGC GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921168.2
CDS	228..6023
Translation	MSHVYEERATMIAAGELQSFVPFGREHCKNHPNAVNLQLRQLQPASELWSSDGAAGLVGSLQEVTLQERDKERWQLRKGTAPDDVEYDEELYVAGNMVIWSRGSKNQASCVYKAFTVDSPVQQALWCNFTVPQAKKDVKDVVEQTVCIVQSTCVNVHSMSGKDFISPLPFQVSALWPTKFGLLLERKNITVDGHMSPIREPLPTLFSMLHPLDEIAPVVCKSTGLFETRLQYVSDSALRVVFTCLKPSIIMTYDTQQCTHTVWNLRKVTSEEQNTVLKFPDQSGPAHTPNAAPSFLSTHLRNVNRLDSPASPLHCHALHNQSRIPASPALHSRVHSPSISNMAALSRAHSPGIAVPSFSGNQRFNMSCHTPSSRGHSILASPNSTLNDTLLPEMEPILPDLCIEQLWTETSAISREKGCQASKVFITSDLCENRYLCFLVESHQQLRCVKFLESNETSQLIFATVSTIAAKDAAPLEDIHTMLVLEANGSLVLYTGVTRVSKVFVPGLLSPTFSVPNQPPILSTPLENISTPARATVQHMSRLEEVGMPSPVLEMRSSLQHNESSWLEESPFQQTAPRIQTLRDPVCNRVTLELSSGSMLRITIPEVATSELVKRCLQAIRFILPKDTAMKVLVKWYNSYNAPGGPSMHLEWNLFVTCLMTMMGYNTERLTWTRNLHFEVPLSPVIAAKKARPADTGSDEDWLYLRCSDYHWQVSMRPAVSVQGLEMLFPPAGVDDQESALGLESSALLFPHIPLLFYVLHLLYEDLKLDELQRSGARALVGLLQQLARDLQLEDFIDLYWRDFPSFIKTFKEACVIDQAQMELMTWPVFLNGEAPCVFNWISRCLRGQDVAPFPYLPGICERTKLLVLSYALYVVGDEDACSTNMLKYLCRLSAGQKKNASDPFLRRQSSVKPISSSGCLAEKLVLWLTSAGFTLKDLETVPFGVALPIRDAIYQCREHPCSDWSEEVCALIGRQDLTRQAHKVTLANRKSTVGLNVMLEPPVSSEADEEEDGMTDLHPEVTALIWSQDLRVQEVRRLLQSSRPVRVNVPHLPEISDHEYIEEKENKLLQLCQRTMSLPVGRGLFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTMVDLNSGNIDVPPNMTSWPSFHNGVAAGLKIAPASQVESAWIAYNKPKSAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHSVQVAAVVGIGLVYQGTGHRHNAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRTHAGINREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSVADWLKAPDTMFLLDFIKPEFLLLRTLARCLIMWDEIFPNADWVTSNVPQIIQENIGNQDQTPVSEDLNMETLVQAHDYIIAGGCLAVGFRFAGSANSDAFDCLYKFAKNFMQCLTTSTATVTGQYNLQTCLSMVLLAVSMVMSGSGNLKVLQLCRFLHKRTFGEMNYGFHMAHHMALGLLFLGGGRYTLSTSNSAIAALLCALYPHFPAHSTDNRYHLQALRHLAVLAAEPRLLVPVDVDSLKPCYALLDVTYKETQWYEETTVELMAPTMLPELHLLKRIKVKGPRYWELNIDISKGTQHLQSILSRDGVLYVKLRAGQLPYKDDPQGWKSLLAPNLNNRSAEVRTFKPEAISTFTSDAALVSFAEHFCRASENMKHRQETVALLSAILYECVTQENPEMLPTYISIEQAVRTLERADLSETFSLWQLKLVLELCESRLARALLLSSEFLPIMKSSADRALDHWLCDNSNVLKEYLRRQTMNGDTDVNMLACFLVYHSIPSLKNMLASDLEGCSSFPELLLKSSQLGVPIRALLRLSPLLLQNHDSGV

Target ORF information:

RefSeq Version	XM_001921133.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921133.4

ORF Insert Sequence:

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGGGAA 
CCTCTCCCGA CTTTGTTCAG CATGCTGCAT CCATTAGATG AAATCGCTCC TGTTGTTTGT 
AAGTCCACCG GTCTGTTTGA AACCCGGCTC CAGTATGTTT CAGACAGCGC TCTGAGAGTG 
GTGTTCACCT GCCTCAAACC CTCCATTATC ATGACCTACG ACACACAGCA GTGCACACAC 
ACCGTCTGGA ATCTGCGCAA AGTCACATCT GAGGAGCAGA ACACTGTGTT AAAGTTTCCA 
GATCAGTCTG GACCGGCTCA CACTCCTAAT GCGGCTCCCA GTTTCCTCAG CACACACCTG 
AGAAACGTGA ACCGGCTGGA CTCTCCGGCT TCACCTTTAC ACTGCCATGC TCTTCACAAC 
CAAAGCCGAA TCCCAGCCTC CCCGGCCCTG CACTCTCGGG TTCACTCGCC CAGCATTTCC 
AACATGGCTG CTCTCAGTCG TGCTCATTCC CCTGGCATCG CCGTGCCCTC GTTCTCTGGG 
AATCAGCGTT TCAACATGTC ATGCCACACG CCATCTTCGC GTGGCCACAG CATCCTGGCT 
TCACCCAACA GCACCCTGAA TGACACCCTG CTGCCTGAGA TGGAGCCCAT CTTACCAGAC 
CTCTGCATCG AGCAGCTGTG GACGGAAACC AGCGCCATCT CCAGGGAGAA AGGCTGCCAG 
GCGTCTAAAG TCTTCATCAC CTCTGATCTG TGTGAAAACC GGTACCTGTG CTTCCTGGTC 
GAGTCCCATC AGCAGCTCAG GTGTGTAAAA TTTCTTGAGA GCAATGAAAC GTCTCAACTG 
ATCTTTGCAA CGGTTTCCAC CATCGCTGCA AAAGATGCCG CTCCTCTAGA GGACATTCAC 
ACAATGCTGG TGCTGGAGGC CAATGGGAGT CTGGTGTTGT ACACAGGAGT CACCAGGGTC 
AGCAAGGTGT TTGTCCCTGG CCTTCTGTCT CCTACATTCA GCGTGCCGAA CCAGCCGCCA 
ATATTGAGCA CACCTCTGGA AAACATCAGC ACTCCGGCCC GAGCCACAGT TCAGCATATG 
AGCCGTCTGG AGGAGGTTGG CATGCCGTCT CCTGTGCTGG AGATGCGCAG CTCGTTGCAG 
CACAATGAGT CCTCATGGCT GGAAGAGTCT CCATTTCAGC AAACTGCTCC TCGTATTCAG 
ACCCTCCGAG ACCCCGTCTG CAACAGAGTC ACACTGGAAT TGAGTTCCGG TTCCATGTTG 
AGAATCACCA TTCCCGAGGT GGCCACTTCA GAGCTGGTCA AACGCTGCCT GCAGGCCATC 
CGATTTATTT TACCCAAGGA CACTGCCATG AAAGTGCTGG TCAAGTGGTA CAACAGCTAC 
AACGCCCCCG GAGGTCCCAG CATGCACCTG GAGTGGAACC TGTTCGTCAC CTGCCTAATG 
ACAATGATGG GTTACAACAC AGAGCGGCTG ACGTGGACAC GCAACCTTCA TTTTGAAGTG 
CCTCTTTCAC CAGTAATCGC TGCTAAGAAA GCCCGTCCTG CCGACACTGG GTCAGATGAG 
GATTGGCTGT ATCTCCGCTG CTCAGACTAT CATTGGCAGG TGTCCATGCG GCCTGCGGTG 
TCTGTTCAGG GTCTGGAGAT GCTGTTTCCC CCTGCTGGAG TGGATGATCA GGAGTCTGCT 
CTGGGTTTGG AGTCGTCCGC TCTCCTTTTC CCACACATCC CGCTGCTCTT TTATGTCCTG 
CATCTGCTGT ATGAGGACCT GAAGCTGGAC GAGTTGCAGA GGAGTGGTGC CAGGGCTCTG 
GTTGGCCTGC TGCAACAGCT TGCCAGGGAC CTGCAGCTGG AGGATTTCAT TGATCTGTAC 
TGGCGTGATT TCCCCTCATT CATCAAAACG TTCAAGGAGG CCTGTGTGAT TGATCAAGCT 
CAGATGGAGC TGATGACGTG GCCGGTGTTT CTGAATGGAG AAGCTCCGTG TGTGTTCAAC 
TGGATCAGTC GCTGTCTGAG AGGACAAGAT GTTGCGCCGT TTCCATATCT GCCTGGCATC 
TGTGAGAGAA CCAAACTACT GGTGCTGAGT TATGCCCTGT ACGTGGTGGG AGATGAAGAC 
GCCTGCTCCA CCAACATGCT CAAATACCTT TGCAGACTGT CAGCAGGGCA AAAGAAGAAT 
GCAAGTGATC CATTTCTGAG AAGGCAGAGC AGTGTGAAGC CCATATCTTC ATCCGGATGC 
CTGGCTGAGA AGCTGGTGCT GTGGTTAACC TCCGCAGGAT TTACACTGAA GGATCTCGAG 
ACGGTGCCGT TCGGTGTGGC TCTGCCAATT AGAGACGCCA TCTATCAGTG CAGAGAACAC 
CCCTGCTCTG ATTGGTCCGA AGAGGTCTGC GCTCTGATTG GCCGACAGGA CCTGACCAGA 
CAAGCTCACA AAGTCACTCT AGCCAATCGT AAATCGACGG TGGGTCTTAA CGTGATGCTG 
GAGCCGCCGG TGAGCAGCGA AGCCGATGAG GAAGAGGACG GCATGACGGA TCTTCACCCC 
GAGGTGACCG CGCTCATCTG GAGTCAAGAT CTCAGGGTGC AGGAGGTGCG GCGGCTGCTC 
CAGAGCTCCA GACCCGTCCG TGTTAATGTG CCACATCTGC CGGAGATCAG TGACCACGAG 
TACATTGAGG AGAAGGAGAA CAAGCTCCTC CAGCTGTGCC AGCGCACCAT GTCTCTCCCA 
GTGGGACGAG GGCTTTTTAC TCTCTTCTCA TACCACCCTG TGCCAACCGA ACCACTGCCT 
GTGCCCAAAC TCAACCTGAC TGGCCGTGCT CCTCCCAGGA ACACCATGGT GGACCTGAAC 
AGTGGAAACA TCGACGTACC ACCCAACATG ACCAGCTGGC CCAGCTTTCA CAACGGCGTG 
GCGGCAGGCC TGAAAATCGC TCCTGCTTCT CAAGTTGAGT CCGCTTGGAT CGCCTACAAC 
AAACCCAAAA GTGCCGAACT GGCCAATGAG TATGCTGGTT TCCTGATGGC ACTGGGCCTC 
AATGGTCACC TCACCAAACT AGCCACTCTC AACATCCATG ACTACCTCAC CAAGGGTCAT 
GAAATGACGA GTATCGGGCT GCTTTTGGGT GTTTCTGCTG CTAAACTGGG CACCATGGAC 
ATGTCCATCA CTCGTCTGCT GAGCATCCAC ATCCCTGCAT TGCTGCCGCC GACCTCCACC 
GAGCTGGACG TCCCACACAG TGTGCAGGTG GCTGCTGTGG TCGGCATCGG CCTGGTCTAT 
CAGGGCACCG GACACCGGCA CAACGCAGAG GTGCTGCTGG CAGAGATCGG TCGTCCGCCG 
GGTCCAGAGA TGGAATACTG CACGGACAGA GAGTCATATT CTCTAGCTGC AGGACTGGCA 
CTCGGCATGG TCTGCCTCGG GCACGGCAGT AACCTGATCG GGATGAGTGA CCTGAACGTG 
CCCGAGCAGC TCTATCAGTA CATGGTGGGA GGTCATCGCC GCACTCATGC TGGCATCAAC 
AGAGAGAAAC ACAAATCTCC CAGCTATCAG ATCAAGGAAG GTGACACCAT CAATGTAGAC 
GTGACTTGTC CTGGGGCTAC ACTTGCTCTG GCCATGATTT ACCTCAAAAC AAATAATAGG 
TCCGTTGCTG ATTGGCTGAA AGCCCCAGAC ACCATGTTCC TGCTGGACTT CATCAAGCCT 
GAGTTCCTGC TGCTGAGGAC GCTGGCCAGA TGCCTTATCA TGTGGGACGA GATCTTTCCC 
AATGCCGACT GGGTGACCAG CAATGTTCCT CAGATCATCC AAGAAAACAT CGGAAACCAG 
GACCAGACGC CCGTCTCGGA GGACCTCAAT ATGGAGACTT TGGTTCAGGC TCATGACTAT 
ATAATCGCCG GAGGCTGTTT GGCTGTGGGA TTTCGGTTTG CTGGCTCTGC TAATTCAGAC 
GCCTTTGACT GCTTATACAA ATTTGCCAAA AACTTCATGC AGTGTTTGAC CACCTCTACT 
GCTACAGTGA CAGGCCAGTA TAACCTGCAG ACGTGTTTGA GCATGGTTCT TCTGGCAGTT 
TCTATGGTCA TGTCCGGCTC AGGGAACCTG AAAGTGCTGC AGTTGTGCCG CTTTCTCCAC 
AAACGCACCT TTGGAGAGAT GAACTATGGG TTCCACATGG CTCATCACAT GGCCCTGGGT 
CTGCTCTTCC TGGGCGGAGG GAGATACACC CTCAGCACCT CCAACTCGGC CATCGCCGCG 
CTGCTCTGTG CACTTTATCC TCATTTTCCA GCTCACAGCA CAGACAACAG GTATCACCTC 
CAGGCGTTGC GTCATCTGGC TGTCCTTGCG GCAGAACCTC GTCTGCTGGT GCCTGTGGAT 
GTGGACTCTC TCAAACCCTG CTACGCTCTG CTGGATGTCA CCTACAAGGA GACGCAGTGG 
TACGAAGAGA CCACCGTGGA GTTGATGGCT CCCACAATGC TGCCCGAACT CCACCTGCTG 
AAGCGGATTA AGGTGAAAGG TCCACGATAC TGGGAGCTGA ACATCGACAT CAGTAAAGGA 
ACCCAGCATC TGCAATCCAT CCTGTCCAGA GACGGTGTCC TGTATGTAAA GTTGCGGGCC 
GGTCAGCTTC CCTACAAGGA CGATCCTCAA GGCTGGAAGA GTTTACTGGC CCCGAACCTC 
AACAACAGAA GCGCAGAAGT TCGCACGTTT AAGCCCGAGG CCATCTCAAC CTTCACCTCA 
GACGCCGCTT TGGTCTCCTT CGCTGAGCAT TTCTGCAGAG CCTCGGAAAA CATGAAGCAT 
AGGCAGGAGA CTGTGGCTTT ACTGTCCGCC ATACTGTACG AGTGCGTGAC GCAAGAAAAC 
CCAGAAATGC TGCCAACGTA CATCTCCATT GAGCAGGCGG TGCGGACTCT GGAGCGGGCC 
GATCTGAGCG AGACATTCAG CCTGTGGCAG CTGAAGCTGG TGCTGGAGTT GTGTGAGAGT 
CGATTGGCTC GAGCTCTGCT GCTCAGCTCG GAGTTTCTGC CTATCATGAA GAGCTCAGCA 
GATCGAGCTC TGGATCACTG GCTCTGTGAC AATAGTAATG TGCTGAAGGA GTACCTAAGA 
CGACAGACGA TGAACGGCGA CACTGATGTC AACATGCTGG CCTGCTTTCT GGTCTATCAT 
TCAATCCCCA GCCTCAAAAA CATGCTGGCG TCCGACCTGG AAGGATGTTC CAGTTTTCCA 
GAGCTGCTCC TCAAGAGCAG TCAGTTGGGT GTTCCCATTC GAGCTCTTCT ACGATTGAGT 
CCTTTACTCC TGCAAAATCA CGACTCTGGC GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANAPC1 ( XM_001921133.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001921168.2 Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X2, mRNA.

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CloneID	ODa19122
Clone ID Related Accession (Same CDS sequence)	XM_001921133.4 , XM_001921133.3
Accession Version	XM_001921133.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	anaphase-promoting complex subunit 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001921133.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGGGAA 
CCTCTCCCGA CTTTGTTCAG CATGCTGCAT CCATTAGATG AAATCGCTCC TGTTGTTTGT 
AAGTCCACCG GTCTGTTTGA AACCCGGCTC CAGTATGTTT CAGACAGCGC TCTGAGAGTG 
GTGTTCACCT GCCTCAAACC CTCCATTATC ATGACCTACG ACACACAGCA GTGCACACAC 
ACCGTCTGGA ATCTGCGCAA AGTCACATCT GAGGAGCAGA ACACTGTGTT AAAGTTTCCA 
GATCAGTCTG GACCGGCTCA CACTCCTAAT GCGGCTCCCA GTTTCCTCAG CACACACCTG 
AGAAACGTGA ACCGGCTGGA CTCTCCGGCT TCACCTTTAC ACTGCCATGC TCTTCACAAC 
CAAAGCCGAA TCCCAGCCTC CCCGGCCCTG CACTCTCGGG TTCACTCGCC CAGCATTTCC 
AACATGGCTG CTCTCAGTCG TGCTCATTCC CCTGGCATCG CCGTGCCCTC GTTCTCTGGG 
AATCAGCGTT TCAACATGTC ATGCCACACG CCATCTTCGC GTGGCCACAG CATCCTGGCT 
TCACCCAACA GCACCCTGAA TGACACCCTG CTGCCTGAGA TGGAGCCCAT CTTACCAGAC 
CTCTGCATCG AGCAGCTGTG GACGGAAACC AGCGCCATCT CCAGGGAGAA AGGCTGCCAG 
GCGTCTAAAG TCTTCATCAC CTCTGATCTG TGTGAAAACC GGTACCTGTG CTTCCTGGTC 
GAGTCCCATC AGCAGCTCAG GTGTGTAAAA TTTCTTGAGA GCAATGAAAC GTCTCAACTG 
ATCTTTGCAA CGGTTTCCAC CATCGCTGCA AAAGATGCCG CTCCTCTAGA GGACATTCAC 
ACAATGCTGG TGCTGGAGGC CAATGGGAGT CTGGTGTTGT ACACAGGAGT CACCAGGGTC 
AGCAAGGTGT TTGTCCCTGG CCTTCTGTCT CCTACATTCA GCGTGCCGAA CCAGCCGCCA 
ATATTGAGCA CACCTCTGGA AAACATCAGC ACTCCGGCCC GAGCCACAGT TCAGCATATG 
AGCCGTCTGG AGGAGGTTGG CATGCCGTCT CCTGTGCTGG AGATGCGCAG CTCGTTGCAG 
CACAATGAGT CCTCATGGCT GGAAGAGTCT CCATTTCAGC AAACTGCTCC TCGTATTCAG 
ACCCTCCGAG ACCCCGTCTG CAACAGAGTC ACACTGGAAT TGAGTTCCGG TTCCATGTTG 
AGAATCACCA TTCCCGAGGT GGCCACTTCA GAGCTGGTCA AACGCTGCCT GCAGGCCATC 
CGATTTATTT TACCCAAGGA CACTGCCATG AAAGTGCTGG TCAAGTGGTA CAACAGCTAC 
AACGCCCCCG GAGGTCCCAG CATGCACCTG GAGTGGAACC TGTTCGTCAC CTGCCTAATG 
ACAATGATGG GTTACAACAC AGAGCGGCTG ACGTGGACAC GCAACCTTCA TTTTGAAGTG 
CCTCTTTCAC CAGTAATCGC TGCTAAGAAA GCCCGTCCTG CCGACACTGG GTCAGATGAG 
GATTGGCTGT ATCTCCGCTG CTCAGACTAT CATTGGCAGG TGTCCATGCG GCCTGCGGTG 
TCTGTTCAGG GTCTGGAGAT GCTGTTTCCC CCTGCTGGAG TGGATGATCA GGAGTCTGCT 
CTGGGTTTGG AGTCGTCCGC TCTCCTTTTC CCACACATCC CGCTGCTCTT TTATGTCCTG 
CATCTGCTGT ATGAGGACCT GAAGCTGGAC GAGTTGCAGA GGAGTGGTGC CAGGGCTCTG 
GTTGGCCTGC TGCAACAGCT TGCCAGGGAC CTGCAGCTGG AGGATTTCAT TGATCTGTAC 
TGGCGTGATT TCCCCTCATT CATCAAAACG TTCAAGGAGG CCTGTGTGAT TGATCAAGCT 
CAGATGGAGC TGATGACGTG GCCGGTGTTT CTGAATGGAG AAGCTCCGTG TGTGTTCAAC 
TGGATCAGTC GCTGTCTGAG AGGACAAGAT GTTGCGCCGT TTCCATATCT GCCTGGCATC 
TGTGAGAGAA CCAAACTACT GGTGCTGAGT TATGCCCTGT ACGTGGTGGG AGATGAAGAC 
GCCTGCTCCA CCAACATGCT CAAATACCTT TGCAGACTGT CAGCAGGGCA AAAGAAGAAT 
GCAAGTGATC CATTTCTGAG AAGGCAGAGC AGTGTGAAGC CCATATCTTC ATCCGGATGC 
CTGGCTGAGA AGCTGGTGCT GTGGTTAACC TCCGCAGGAT TTACACTGAA GGATCTCGAG 
ACGGTGCCGT TCGGTGTGGC TCTGCCAATT AGAGACGCCA TCTATCAGTG CAGAGAACAC 
CCCTGCTCTG ATTGGTCCGA AGAGGTCTGC GCTCTGATTG GCCGACAGGA CCTGACCAGA 
CAAGCTCACA AAGTCACTCT AGCCAATCGT AAATCGACGG TGGGTCTTAA CGTGATGCTG 
GAGCCGCCGG TGAGCAGCGA AGCCGATGAG GAAGAGGACG GCATGACGGA TCTTCACCCC 
GAGGTGACCG CGCTCATCTG GAGTCAAGAT CTCAGGGTGC AGGAGGTGCG GCGGCTGCTC 
CAGAGCTCCA GACCCGTCCG TGTTAATGTG CCACATCTGC CGGAGATCAG TGACCACGAG 
TACATTGAGG AGAAGGAGAA CAAGCTCCTC CAGCTGTGCC AGCGCACCAT GTCTCTCCCA 
GTGGGACGAG GGCTTTTTAC TCTCTTCTCA TACCACCCTG TGCCAACCGA ACCACTGCCT 
GTGCCCAAAC TCAACCTGAC TGGCCGTGCT CCTCCCAGGA ACACCATGGT GGACCTGAAC 
AGTGGAAACA TCGACGTACC ACCCAACATG ACCAGCTGGC CCAGCTTTCA CAACGGCGTG 
GCGGCAGGCC TGAAAATCGC TCCTGCTTCT CAAGTTGAGT CCGCTTGGAT CGCCTACAAC 
AAACCCAAAA GTGCCGAACT GGCCAATGAG TATGCTGGTT TCCTGATGGC ACTGGGCCTC 
AATGGTCACC TCACCAAACT AGCCACTCTC AACATCCATG ACTACCTCAC CAAGGGTCAT 
GAAATGACGA GTATCGGGCT GCTTTTGGGT GTTTCTGCTG CTAAACTGGG CACCATGGAC 
ATGTCCATCA CTCGTCTGCT GAGCATCCAC ATCCCTGCAT TGCTGCCGCC GACCTCCACC 
GAGCTGGACG TCCCACACAG TGTGCAGGTG GCTGCTGTGG TCGGCATCGG CCTGGTCTAT 
CAGGGCACCG GACACCGGCA CAACGCAGAG GTGCTGCTGG CAGAGATCGG TCGTCCGCCG 
GGTCCAGAGA TGGAATACTG CACGGACAGA GAGTCATATT CTCTAGCTGC AGGACTGGCA 
CTCGGCATGG TCTGCCTCGG GCACGGCAGT AACCTGATCG GGATGAGTGA CCTGAACGTG 
CCCGAGCAGC TCTATCAGTA CATGGTGGGA GGTCATCGCC GCACTCATGC TGGCATCAAC 
AGAGAGAAAC ACAAATCTCC CAGCTATCAG ATCAAGGAAG GTGACACCAT CAATGTAGAC 
GTGACTTGTC CTGGGGCTAC ACTTGCTCTG GCCATGATTT ACCTCAAAAC AAATAATAGG 
TCCGTTGCTG ATTGGCTGAA AGCCCCAGAC ACCATGTTCC TGCTGGACTT CATCAAGCCT 
GAGTTCCTGC TGCTGAGGAC GCTGGCCAGA TGCCTTATCA TGTGGGACGA GATCTTTCCC 
AATGCCGACT GGGTGACCAG CAATGTTCCT CAGATCATCC AAGAAAACAT CGGAAACCAG 
GACCAGACGC CCGTCTCGGA GGACCTCAAT ATGGAGACTT TGGTTCAGGC TCATGACTAT 
ATAATCGCCG GAGGCTGTTT GGCTGTGGGA TTTCGGTTTG CTGGCTCTGC TAATTCAGAC 
GCCTTTGACT GCTTATACAA ATTTGCCAAA AACTTCATGC AGTGTTTGAC CACCTCTACT 
GCTACAGTGA CAGGCCAGTA TAACCTGCAG ACGTGTTTGA GCATGGTTCT TCTGGCAGTT 
TCTATGGTCA TGTCCGGCTC AGGGAACCTG AAAGTGCTGC AGTTGTGCCG CTTTCTCCAC 
AAACGCACCT TTGGAGAGAT GAACTATGGG TTCCACATGG CTCATCACAT GGCCCTGGGT 
CTGCTCTTCC TGGGCGGAGG GAGATACACC CTCAGCACCT CCAACTCGGC CATCGCCGCG 
CTGCTCTGTG CACTTTATCC TCATTTTCCA GCTCACAGCA CAGACAACAG GTATCACCTC 
CAGGCGTTGC GTCATCTGGC TGTCCTTGCG GCAGAACCTC GTCTGCTGGT GCCTGTGGAT 
GTGGACTCTC TCAAACCCTG CTACGCTCTG CTGGATGTCA CCTACAAGGA GACGCAGTGG 
TACGAAGAGA CCACCGTGGA GTTGATGGCT CCCACAATGC TGCCCGAACT CCACCTGCTG 
AAGCGGATTA AGGTGAAAGG TCCACGATAC TGGGAGCTGA ACATCGACAT CAGTAAAGGA 
ACCCAGCATC TGCAATCCAT CCTGTCCAGA GACGGTGTCC TGTATGTAAA GTTGCGGGCC 
GGTCAGCTTC CCTACAAGGA CGATCCTCAA GGCTGGAAGA GTTTACTGGC CCCGAACCTC 
AACAACAGAA GCGCAGAAGT TCGCACGTTT AAGCCCGAGG CCATCTCAAC CTTCACCTCA 
GACGCCGCTT TGGTCTCCTT CGCTGAGCAT TTCTGCAGAG CCTCGGAAAA CATGAAGCAT 
AGGCAGGAGA CTGTGGCTTT ACTGTCCGCC ATACTGTACG AGTGCGTGAC GCAAGAAAAC 
CCAGAAATGC TGCCAACGTA CATCTCCATT GAGCAGGCGG TGCGGACTCT GGAGCGGGCC 
GATCTGAGCG AGACATTCAG CCTGTGGCAG CTGAAGCTGG TGCTGGAGTT GTGTGAGAGT 
CGATTGGCTC GAGCTCTGCT GCTCAGCTCG GAGTTTCTGC CTATCATGAA GAGCTCAGCA 
GATCGAGCTC TGGATCACTG GCTCTGTGAC AATAGTAATG TGCTGAAGGA GTACCTAAGA 
CGACAGACGA TGAACGGCGA CACTGATGTC AACATGCTGG CCTGCTTTCT GGTCTATCAT 
TCAATCCCCA GCCTCAAAAA CATGCTGGCG TCCGACCTGG AAGGATGTTC CAGTTTTCCA 
GAGCTGCTCC TCAAGAGCAG TCAGTTGGGT GTTCCCATTC GAGCTCTTCT ACGATTGAGT 
CCTTTACTCC TGCAAAATCA CGACTCTGGC GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921168.2
CDS	216..6011
Translation	MSHVYEERATMIAAGELQSFVPFGREHCKNHPNAVNLQLRQLQPASELWSSDGAAGLVGSLQEVTLQERDKERWQLRKGTAPDDVEYDEELYVAGNMVIWSRGSKNQASCVYKAFTVDSPVQQALWCNFTVPQAKKDVKDVVEQTVCIVQSTCVNVHSMSGKDFISPLPFQVSALWPTKFGLLLERKNITVDGHMSPIREPLPTLFSMLHPLDEIAPVVCKSTGLFETRLQYVSDSALRVVFTCLKPSIIMTYDTQQCTHTVWNLRKVTSEEQNTVLKFPDQSGPAHTPNAAPSFLSTHLRNVNRLDSPASPLHCHALHNQSRIPASPALHSRVHSPSISNMAALSRAHSPGIAVPSFSGNQRFNMSCHTPSSRGHSILASPNSTLNDTLLPEMEPILPDLCIEQLWTETSAISREKGCQASKVFITSDLCENRYLCFLVESHQQLRCVKFLESNETSQLIFATVSTIAAKDAAPLEDIHTMLVLEANGSLVLYTGVTRVSKVFVPGLLSPTFSVPNQPPILSTPLENISTPARATVQHMSRLEEVGMPSPVLEMRSSLQHNESSWLEESPFQQTAPRIQTLRDPVCNRVTLELSSGSMLRITIPEVATSELVKRCLQAIRFILPKDTAMKVLVKWYNSYNAPGGPSMHLEWNLFVTCLMTMMGYNTERLTWTRNLHFEVPLSPVIAAKKARPADTGSDEDWLYLRCSDYHWQVSMRPAVSVQGLEMLFPPAGVDDQESALGLESSALLFPHIPLLFYVLHLLYEDLKLDELQRSGARALVGLLQQLARDLQLEDFIDLYWRDFPSFIKTFKEACVIDQAQMELMTWPVFLNGEAPCVFNWISRCLRGQDVAPFPYLPGICERTKLLVLSYALYVVGDEDACSTNMLKYLCRLSAGQKKNASDPFLRRQSSVKPISSSGCLAEKLVLWLTSAGFTLKDLETVPFGVALPIRDAIYQCREHPCSDWSEEVCALIGRQDLTRQAHKVTLANRKSTVGLNVMLEPPVSSEADEEEDGMTDLHPEVTALIWSQDLRVQEVRRLLQSSRPVRVNVPHLPEISDHEYIEEKENKLLQLCQRTMSLPVGRGLFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTMVDLNSGNIDVPPNMTSWPSFHNGVAAGLKIAPASQVESAWIAYNKPKSAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHSVQVAAVVGIGLVYQGTGHRHNAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRTHAGINREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSVADWLKAPDTMFLLDFIKPEFLLLRTLARCLIMWDEIFPNADWVTSNVPQIIQENIGNQDQTPVSEDLNMETLVQAHDYIIAGGCLAVGFRFAGSANSDAFDCLYKFAKNFMQCLTTSTATVTGQYNLQTCLSMVLLAVSMVMSGSGNLKVLQLCRFLHKRTFGEMNYGFHMAHHMALGLLFLGGGRYTLSTSNSAIAALLCALYPHFPAHSTDNRYHLQALRHLAVLAAEPRLLVPVDVDSLKPCYALLDVTYKETQWYEETTVELMAPTMLPELHLLKRIKVKGPRYWELNIDISKGTQHLQSILSRDGVLYVKLRAGQLPYKDDPQGWKSLLAPNLNNRSAEVRTFKPEAISTFTSDAALVSFAEHFCRASENMKHRQETVALLSAILYECVTQENPEMLPTYISIEQAVRTLERADLSETFSLWQLKLVLELCESRLARALLLSSEFLPIMKSSADRALDHWLCDNSNVLKEYLRRQTMNGDTDVNMLACFLVYHSIPSLKNMLASDLEGCSSFPELLLKSSQLGVPIRALLRLSPLLLQNHDSGV

Target ORF information:

RefSeq Version	XM_001921133.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921133.3

ORF Insert Sequence:

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGGGAA 
CCTCTCCCGA CTTTGTTCAG CATGCTGCAT CCATTAGATG AAATCGCTCC TGTTGTTTGT 
AAGTCCACCG GTCTGTTTGA AACCCGGCTC CAGTATGTTT CAGACAGCGC TCTGAGAGTG 
GTGTTCACCT GCCTCAAACC CTCCATTATC ATGACCTACG ACACACAGCA GTGCACACAC 
ACCGTCTGGA ATCTGCGCAA AGTCACATCT GAGGAGCAGA ACACTGTGTT AAAGTTTCCA 
GATCAGTCTG GACCGGCTCA CACTCCTAAT GCGGCTCCCA GTTTCCTCAG CACACACCTG 
AGAAACGTGA ACCGGCTGGA CTCTCCGGCT TCACCTTTAC ACTGCCATGC TCTTCACAAC 
CAAAGCCGAA TCCCAGCCTC CCCGGCCCTG CACTCTCGGG TTCACTCGCC CAGCATTTCC 
AACATGGCTG CTCTCAGTCG TGCTCATTCC CCTGGCATCG CCGTGCCCTC GTTCTCTGGG 
AATCAGCGTT TCAACATGTC ATGCCACACG CCATCTTCGC GTGGCCACAG CATCCTGGCT 
TCACCCAACA GCACCCTGAA TGACACCCTG CTGCCTGAGA TGGAGCCCAT CTTACCAGAC 
CTCTGCATCG AGCAGCTGTG GACGGAAACC AGCGCCATCT CCAGGGAGAA AGGCTGCCAG 
GCGTCTAAAG TCTTCATCAC CTCTGATCTG TGTGAAAACC GGTACCTGTG CTTCCTGGTC 
GAGTCCCATC AGCAGCTCAG GTGTGTAAAA TTTCTTGAGA GCAATGAAAC GTCTCAACTG 
ATCTTTGCAA CGGTTTCCAC CATCGCTGCA AAAGATGCCG CTCCTCTAGA GGACATTCAC 
ACAATGCTGG TGCTGGAGGC CAATGGGAGT CTGGTGTTGT ACACAGGAGT CACCAGGGTC 
AGCAAGGTGT TTGTCCCTGG CCTTCTGTCT CCTACATTCA GCGTGCCGAA CCAGCCGCCA 
ATATTGAGCA CACCTCTGGA AAACATCAGC ACTCCGGCCC GAGCCACAGT TCAGCATATG 
AGCCGTCTGG AGGAGGTTGG CATGCCGTCT CCTGTGCTGG AGATGCGCAG CTCGTTGCAG 
CACAATGAGT CCTCATGGCT GGAAGAGTCT CCATTTCAGC AAACTGCTCC TCGTATTCAG 
ACCCTCCGAG ACCCCGTCTG CAACAGAGTC ACACTGGAAT TGAGTTCCGG TTCCATGTTG 
AGAATCACCA TTCCCGAGGT GGCCACTTCA GAGCTGGTCA AACGCTGCCT GCAGGCCATC 
CGATTTATTT TACCCAAGGA CACTGCCATG AAAGTGCTGG TCAAGTGGTA CAACAGCTAC 
AACGCCCCCG GAGGTCCCAG CATGCACCTG GAGTGGAACC TGTTCGTCAC CTGCCTAATG 
ACAATGATGG GTTACAACAC AGAGCGGCTG ACGTGGACAC GCAACCTTCA TTTTGAAGTG 
CCTCTTTCAC CAGTAATCGC TGCTAAGAAA GCCCGTCCTG CCGACACTGG GTCAGATGAG 
GATTGGCTGT ATCTCCGCTG CTCAGACTAT CATTGGCAGG TGTCCATGCG GCCTGCGGTG 
TCTGTTCAGG GTCTGGAGAT GCTGTTTCCC CCTGCTGGAG TGGATGATCA GGAGTCTGCT 
CTGGGTTTGG AGTCGTCCGC TCTCCTTTTC CCACACATCC CGCTGCTCTT TTATGTCCTG 
CATCTGCTGT ATGAGGACCT GAAGCTGGAC GAGTTGCAGA GGAGTGGTGC CAGGGCTCTG 
GTTGGCCTGC TGCAACAGCT TGCCAGGGAC CTGCAGCTGG AGGATTTCAT TGATCTGTAC 
TGGCGTGATT TCCCCTCATT CATCAAAACG TTCAAGGAGG CCTGTGTGAT TGATCAAGCT 
CAGATGGAGC TGATGACGTG GCCGGTGTTT CTGAATGGAG AAGCTCCGTG TGTGTTCAAC 
TGGATCAGTC GCTGTCTGAG AGGACAAGAT GTTGCGCCGT TTCCATATCT GCCTGGCATC 
TGTGAGAGAA CCAAACTACT GGTGCTGAGT TATGCCCTGT ACGTGGTGGG AGATGAAGAC 
GCCTGCTCCA CCAACATGCT CAAATACCTT TGCAGACTGT CAGCAGGGCA AAAGAAGAAT 
GCAAGTGATC CATTTCTGAG AAGGCAGAGC AGTGTGAAGC CCATATCTTC ATCCGGATGC 
CTGGCTGAGA AGCTGGTGCT GTGGTTAACC TCCGCAGGAT TTACACTGAA GGATCTCGAG 
ACGGTGCCGT TCGGTGTGGC TCTGCCAATT AGAGACGCCA TCTATCAGTG CAGAGAACAC 
CCCTGCTCTG ATTGGTCCGA AGAGGTCTGC GCTCTGATTG GCCGACAGGA CCTGACCAGA 
CAAGCTCACA AAGTCACTCT AGCCAATCGT AAATCGACGG TGGGTCTTAA CGTGATGCTG 
GAGCCGCCGG TGAGCAGCGA AGCCGATGAG GAAGAGGACG GCATGACGGA TCTTCACCCC 
GAGGTGACCG CGCTCATCTG GAGTCAAGAT CTCAGGGTGC AGGAGGTGCG GCGGCTGCTC 
CAGAGCTCCA GACCCGTCCG TGTTAATGTG CCACATCTGC CGGAGATCAG TGACCACGAG 
TACATTGAGG AGAAGGAGAA CAAGCTCCTC CAGCTGTGCC AGCGCACCAT GTCTCTCCCA 
GTGGGACGAG GGCTTTTTAC TCTCTTCTCA TACCACCCTG TGCCAACCGA ACCACTGCCT 
GTGCCCAAAC TCAACCTGAC TGGCCGTGCT CCTCCCAGGA ACACCATGGT GGACCTGAAC 
AGTGGAAACA TCGACGTACC ACCCAACATG ACCAGCTGGC CCAGCTTTCA CAACGGCGTG 
GCGGCAGGCC TGAAAATCGC TCCTGCTTCT CAAGTTGAGT CCGCTTGGAT CGCCTACAAC 
AAACCCAAAA GTGCCGAACT GGCCAATGAG TATGCTGGTT TCCTGATGGC ACTGGGCCTC 
AATGGTCACC TCACCAAACT AGCCACTCTC AACATCCATG ACTACCTCAC CAAGGGTCAT 
GAAATGACGA GTATCGGGCT GCTTTTGGGT GTTTCTGCTG CTAAACTGGG CACCATGGAC 
ATGTCCATCA CTCGTCTGCT GAGCATCCAC ATCCCTGCAT TGCTGCCGCC GACCTCCACC 
GAGCTGGACG TCCCACACAG TGTGCAGGTG GCTGCTGTGG TCGGCATCGG CCTGGTCTAT 
CAGGGCACCG GACACCGGCA CAACGCAGAG GTGCTGCTGG CAGAGATCGG TCGTCCGCCG 
GGTCCAGAGA TGGAATACTG CACGGACAGA GAGTCATATT CTCTAGCTGC AGGACTGGCA 
CTCGGCATGG TCTGCCTCGG GCACGGCAGT AACCTGATCG GGATGAGTGA CCTGAACGTG 
CCCGAGCAGC TCTATCAGTA CATGGTGGGA GGTCATCGCC GCACTCATGC TGGCATCAAC 
AGAGAGAAAC ACAAATCTCC CAGCTATCAG ATCAAGGAAG GTGACACCAT CAATGTAGAC 
GTGACTTGTC CTGGGGCTAC ACTTGCTCTG GCCATGATTT ACCTCAAAAC AAATAATAGG 
TCCGTTGCTG ATTGGCTGAA AGCCCCAGAC ACCATGTTCC TGCTGGACTT CATCAAGCCT 
GAGTTCCTGC TGCTGAGGAC GCTGGCCAGA TGCCTTATCA TGTGGGACGA GATCTTTCCC 
AATGCCGACT GGGTGACCAG CAATGTTCCT CAGATCATCC AAGAAAACAT CGGAAACCAG 
GACCAGACGC CCGTCTCGGA GGACCTCAAT ATGGAGACTT TGGTTCAGGC TCATGACTAT 
ATAATCGCCG GAGGCTGTTT GGCTGTGGGA TTTCGGTTTG CTGGCTCTGC TAATTCAGAC 
GCCTTTGACT GCTTATACAA ATTTGCCAAA AACTTCATGC AGTGTTTGAC CACCTCTACT 
GCTACAGTGA CAGGCCAGTA TAACCTGCAG ACGTGTTTGA GCATGGTTCT TCTGGCAGTT 
TCTATGGTCA TGTCCGGCTC AGGGAACCTG AAAGTGCTGC AGTTGTGCCG CTTTCTCCAC 
AAACGCACCT TTGGAGAGAT GAACTATGGG TTCCACATGG CTCATCACAT GGCCCTGGGT 
CTGCTCTTCC TGGGCGGAGG GAGATACACC CTCAGCACCT CCAACTCGGC CATCGCCGCG 
CTGCTCTGTG CACTTTATCC TCATTTTCCA GCTCACAGCA CAGACAACAG GTATCACCTC 
CAGGCGTTGC GTCATCTGGC TGTCCTTGCG GCAGAACCTC GTCTGCTGGT GCCTGTGGAT 
GTGGACTCTC TCAAACCCTG CTACGCTCTG CTGGATGTCA CCTACAAGGA GACGCAGTGG 
TACGAAGAGA CCACCGTGGA GTTGATGGCT CCCACAATGC TGCCCGAACT CCACCTGCTG 
AAGCGGATTA AGGTGAAAGG TCCACGATAC TGGGAGCTGA ACATCGACAT CAGTAAAGGA 
ACCCAGCATC TGCAATCCAT CCTGTCCAGA GACGGTGTCC TGTATGTAAA GTTGCGGGCC 
GGTCAGCTTC CCTACAAGGA CGATCCTCAA GGCTGGAAGA GTTTACTGGC CCCGAACCTC 
AACAACAGAA GCGCAGAAGT TCGCACGTTT AAGCCCGAGG CCATCTCAAC CTTCACCTCA 
GACGCCGCTT TGGTCTCCTT CGCTGAGCAT TTCTGCAGAG CCTCGGAAAA CATGAAGCAT 
AGGCAGGAGA CTGTGGCTTT ACTGTCCGCC ATACTGTACG AGTGCGTGAC GCAAGAAAAC 
CCAGAAATGC TGCCAACGTA CATCTCCATT GAGCAGGCGG TGCGGACTCT GGAGCGGGCC 
GATCTGAGCG AGACATTCAG CCTGTGGCAG CTGAAGCTGG TGCTGGAGTT GTGTGAGAGT 
CGATTGGCTC GAGCTCTGCT GCTCAGCTCG GAGTTTCTGC CTATCATGAA GAGCTCAGCA 
GATCGAGCTC TGGATCACTG GCTCTGTGAC AATAGTAATG TGCTGAAGGA GTACCTAAGA 
CGACAGACGA TGAACGGCGA CACTGATGTC AACATGCTGG CCTGCTTTCT GGTCTATCAT 
TCAATCCCCA GCCTCAAAAA CATGCTGGCG TCCGACCTGG AAGGATGTTC CAGTTTTCCA 
GAGCTGCTCC TCAAGAGCAG TCAGTTGGGT GTTCCCATTC GAGCTCTTCT ACGATTGAGT 
CCTTTACTCC TGCAAAATCA CGACTCTGGC GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANAPC1 ( XM_005157067.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157124.1 Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X3, mRNA.

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CloneID	ODa19123
Clone ID Related Accession (Same CDS sequence)	XM_005157067.2 , XM_005157067.3
Accession Version	XM_005157067.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	anaphase-promoting complex subunit 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157067.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGCAGG 
GAACCTCTCC CGACTTTGTT CAGCATGCTG CATCCATTAG ATGAAATCGC TCCTGTTGTT 
TGTCTGTTTG AAACCCGGCT CCAGTATGTT TCAGACAGCG CTCTGAGAGT GGTGTTCACC 
TGCCTCAAAC CCTCCATTAT CATGACCTAC GACACACAGC AGTGCACACA CACCGTCTGG 
AATCTGCGCA AAGTCACATC TGAGGAGCAG AACACTGTGT TAAAGTTTCC AGATCAGTCT 
GGACCGGCTC ACACTCCTAA TGCGGCTCCC AGTTTCCTCA GCACACACCT GAGAAACGTG 
AACCGGCTGG ACTCTCCGGC TTCACCTTTA CACTGCCATG CTCTTCACAA CCAAAGCCGA 
ATCCCAGCCT CCCCGGCCCT GCACTCTCGG GTTCACTCGC CCAGCATTTC CAACATGGCT 
GCTCTCAGTC GTGCTCATTC CCCTGGCATC GCCGTGCCCT CGTTCTCTGG GAATCAGCGT 
TTCAACATGT CATGCCACAC GCCATCTTCG CGTGGCCACA GCATCCTGGC TTCACCCAAC 
AGCACCCTGA ATGACACCCT GCTGCCTGAG ATGGAGCCCA TCTTACCAGA CCTCTGCATC 
GAGCAGCTGT GGACGGAAAC CAGCGCCATC TCCAGGGAGA AAGGCTGCCA GGCGTCTAAA 
GTCTTCATCA CCTCTGATCT GTGTGAAAAC CGGTACCTGT GCTTCCTGGT CGAGTCCCAT 
CAGCAGCTCA GGTGTGTAAA ATTTCTTGAG AGCAATGAAA CGTCTCAACT GATCTTTGCA 
ACGGTTTCCA CCATCGCTGC AAAAGATGCC GCTCCTCTAG AGGACATTCA CACAATGCTG 
GTGCTGGAGG CCAATGGGAG TCTGGTGTTG TACACAGGAG TCACCAGGGT CAGCAAGGTG 
TTTGTCCCTG GCCTTCTGTC TCCTACATTC AGCGTGCCGA ACCAGCCGCC AATATTGAGC 
ACACCTCTGG AAAACATCAG CACTCCGGCC CGAGCCACAG TTCAGCATAT GAGCCGTCTG 
GAGGAGGTTG GCATGCCGTC TCCTGTGCTG GAGATGCGCA GCTCGTTGCA GCACAATGAG 
TCCTCATGGC TGGAAGAGTC TCCATTTCAG CAAACTGCTC CTCGTATTCA GACCCTCCGA 
GACCCCGTCT GCAACAGAGT CACACTGGAA TTGAGTTCCG GTTCCATGTT GAGAATCACC 
ATTCCCGAGG TGGCCACTTC AGAGCTGGTC AAACGCTGCC TGCAGGCCAT CCGATTTATT 
TTACCCAAGG ACACTGCCAT GAAAGTGCTG GTCAAGTGGT ACAACAGCTA CAACGCCCCC 
GGAGGTCCCA GCATGCACCT GGAGTGGAAC CTGTTCGTCA CCTGCCTAAT GACAATGATG 
GGTTACAACA CAGAGCGGCT GACGTGGACA CGCAACCTTC ATTTTGAAGT GCCTCTTTCA 
CCAGTAATCG CTGCTAAGAA AGCCCGTCCT GCCGACACTG GGTCAGATGA GGATTGGCTG 
TATCTCCGCT GCTCAGACTA TCATTGGCAG GTGTCCATGC GGCCTGCGGT GTCTGTTCAG 
GGTCTGGAGA TGCTGTTTCC CCCTGCTGGA GTGGATGATC AGGAGTCTGC TCTGGGTTTG 
GAGTCGTCCG CTCTCCTTTT CCCACACATC CCGCTGCTCT TTTATGTCCT GCATCTGCTG 
TATGAGGACC TGAAGCTGGA CGAGTTGCAG AGGAGTGGTG CCAGGGCTCT GGTTGGCCTG 
CTGCAACAGC TTGCCAGGGA CCTGCAGCTG GAGGATTTCA TTGATCTGTA CTGGCGTGAT 
TTCCCCTCAT TCATCAAAAC GTTCAAGGAG GCCTGTGTGA TTGATCAAGC TCAGATGGAG 
CTGATGACGT GGCCGGTGTT TCTGAATGGA GAAGCTCCGT GTGTGTTCAA CTGGATCAGT 
CGCTGTCTGA GAGGACAAGA TGTTGCGCCG TTTCCATATC TGCCTGGCAT CTGTGAGAGA 
ACCAAACTAC TGGTGCTGAG TTATGCCCTG TACGTGGTGG GAGATGAAGA CGCCTGCTCC 
ACCAACATGC TCAAATACCT TTGCAGACTG TCAGCAGGGC AAAAGAAGAA TGCAAGTGAT 
CCATTTCTGA GAAGGCAGAG CAGTGTGAAG CCCATATCTT CATCCGGATG CCTGGCTGAG 
AAGCTGGTGC TGTGGTTAAC CTCCGCAGGA TTTACACTGA AGGATCTCGA GACGGTGCCG 
TTCGGTGTGG CTCTGCCAAT TAGAGACGCC ATCTATCAGT GCAGAGAACA CCCCTGCTCT 
GATTGGTCCG AAGAGGTCTG CGCTCTGATT GGCCGACAGG ACCTGACCAG ACAAGCTCAC 
AAAGTCACTC TAGCCAATCG TAAATCGACG GTGGGTCTTA ACGTGATGCT GGAGCCGCCG 
GTGAGCAGCG AAGCCGATGA GGAAGAGGAC GGCATGACGG ATCTTCACCC CGAGGTGACC 
GCGCTCATCT GGAGTCAAGA TCTCAGGGTG CAGGAGGTGC GGCGGCTGCT CCAGAGCTCC 
AGACCCGTCC GTGTTAATGT GCCACATCTG CCGGAGATCA GTGACCACGA GTACATTGAG 
GAGAAGGAGA ACAAGCTCCT CCAGCTGTGC CAGCGCACCA TGTCTCTCCC AGTGGGACGA 
GGGCTTTTTA CTCTCTTCTC ATACCACCCT GTGCCAACCG AACCACTGCC TGTGCCCAAA 
CTCAACCTGA CTGGCCGTGC TCCTCCCAGG AACACCATGG TGGACCTGAA CAGTGGAAAC 
ATCGACGTAC CACCCAACAT GACCAGCTGG CCCAGCTTTC ACAACGGCGT GGCGGCAGGC 
CTGAAAATCG CTCCTGCTTC TCAAGTTGAG TCCGCTTGGA TCGCCTACAA CAAACCCAAA 
AGTGCCGAAC TGGCCAATGA GTATGCTGGT TTCCTGATGG CACTGGGCCT CAATGGTCAC 
CTCACCAAAC TAGCCACTCT CAACATCCAT GACTACCTCA CCAAGGGTCA TGAAATGACG 
AGTATCGGGC TGCTTTTGGG TGTTTCTGCT GCTAAACTGG GCACCATGGA CATGTCCATC 
ACTCGTCTGC TGAGCATCCA CATCCCTGCA TTGCTGCCGC CGACCTCCAC CGAGCTGGAC 
GTCCCACACA GTGTGCAGGT GGCTGCTGTG GTCGGCATCG GCCTGGTCTA TCAGGGCACC 
GGACACCGGC ACAACGCAGA GGTGCTGCTG GCAGAGATCG GTCGTCCGCC GGGTCCAGAG 
ATGGAATACT GCACGGACAG AGAGTCATAT TCTCTAGCTG CAGGACTGGC ACTCGGCATG 
GTCTGCCTCG GGCACGGCAG TAACCTGATC GGGATGAGTG ACCTGAACGT GCCCGAGCAG 
CTCTATCAGT ACATGGTGGG AGGTCATCGC CGCACTCATG CTGGCATCAA CAGAGAGAAA 
CACAAATCTC CCAGCTATCA GATCAAGGAA GGTGACACCA TCAATGTAGA CGTGACTTGT 
CCTGGGGCTA CACTTGCTCT GGCCATGATT TACCTCAAAA CAAATAATAG GTCCGTTGCT 
GATTGGCTGA AAGCCCCAGA CACCATGTTC CTGCTGGACT TCATCAAGCC TGAGTTCCTG 
CTGCTGAGGA CGCTGGCCAG ATGCCTTATC ATGTGGGACG AGATCTTTCC CAATGCCGAC 
TGGGTGACCA GCAATGTTCC TCAGATCATC CAAGAAAACA TCGGAAACCA GGACCAGACG 
CCCGTCTCGG AGGACCTCAA TATGGAGACT TTGGTTCAGG CTCATGACTA TATAATCGCC 
GGAGGCTGTT TGGCTGTGGG ATTTCGGTTT GCTGGCTCTG CTAATTCAGA CGCCTTTGAC 
TGCTTATACA AATTTGCCAA AAACTTCATG CAGTGTTTGA CCACCTCTAC TGCTACAGTG 
ACAGGCCAGT ATAACCTGCA GACGTGTTTG AGCATGGTTC TTCTGGCAGT TTCTATGGTC 
ATGTCCGGCT CAGGGAACCT GAAAGTGCTG CAGTTGTGCC GCTTTCTCCA CAAACGCACC 
TTTGGAGAGA TGAACTATGG GTTCCACATG GCTCATCACA TGGCCCTGGG TCTGCTCTTC 
CTGGGCGGAG GGAGATACAC CCTCAGCACC TCCAACTCGG CCATCGCCGC GCTGCTCTGT 
GCACTTTATC CTCATTTTCC AGCTCACAGC ACAGACAACA GGTATCACCT CCAGGCGTTG 
CGTCATCTGG CTGTCCTTGC GGCAGAACCT CGTCTGCTGG TGCCTGTGGA TGTGGACTCT 
CTCAAACCCT GCTACGCTCT GCTGGATGTC ACCTACAAGG AGACGCAGTG GTACGAAGAG 
ACCACCGTGG AGTTGATGGC TCCCACAATG CTGCCCGAAC TCCACCTGCT GAAGCGGATT 
AAGGTGAAAG GTCCACGATA CTGGGAGCTG AACATCGACA TCAGTAAAGG AACCCAGCAT 
CTGCAATCCA TCCTGTCCAG AGACGGTGTC CTGTATGTAA AGTTGCGGGC CGGTCAGCTT 
CCCTACAAGG ACGATCCTCA AGGCTGGAAG AGTTTACTGG CCCCGAACCT CAACAACAGA 
AGCGCAGAAG TTCGCACGTT TAAGCCCGAG GCCATCTCAA CCTTCACCTC AGACGCCGCT 
TTGGTCTCCT TCGCTGAGCA TTTCTGCAGA GCCTCGGAAA ACATGAAGCA TAGGCAGGAG 
ACTGTGGCTT TACTGTCCGC CATACTGTAC GAGTGCGTGA CGCAAGAAAA CCCAGAAATG 
CTGCCAACGT ACATCTCCAT TGAGCAGGCG GTGCGGACTC TGGAGCGGGC CGATCTGAGC 
GAGACATTCA GCCTGTGGCA GCTGAAGCTG GTGCTGGAGT TGTGTGAGAG TCGATTGGCT 
CGAGCTCTGC TGCTCAGCTC GGAGTTTCTG CCTATCATGA AGAGCTCAGC AGATCGAGCT 
CTGGATCACT GGCTCTGTGA CAATAGTAAT GTGCTGAAGG AGTACCTAAG ACGACAGACG 
ATGAACGGCG ACACTGATGT CAACATGCTG GCCTGCTTTC TGGTCTATCA TTCAATCCCC 
AGCCTCAAAA ACATGCTGGC GTCCGACCTG GAAGGATGTT CCAGTTTTCC AGAGCTGCTC 
CTCAAGAGCA GTCAGTTGGG TGTTCCCATT CGAGCTCTTC TACGATTGAG TCCTTTACTC 
CTGCAAAATC ACGACTCTGG CGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157124.1
CDS	216..6002
Translation	MSHVYEERATMIAAGELQSFVPFGREHCKNHPNAVNLQLRQLQPASELWSSDGAAGLVGSLQEVTLQERDKERWQLRKGTAPDDVEYDEELYVAGNMVIWSRGSKNQASCVYKAFTVDSPVQQALWCNFTVPQAKKDVKDVVEQTVCIVQSTCVNVHSMSGKDFISPLPFQVSALWPTKFGLLLERKNITVDGHMSPISREPLPTLFSMLHPLDEIAPVVCLFETRLQYVSDSALRVVFTCLKPSIIMTYDTQQCTHTVWNLRKVTSEEQNTVLKFPDQSGPAHTPNAAPSFLSTHLRNVNRLDSPASPLHCHALHNQSRIPASPALHSRVHSPSISNMAALSRAHSPGIAVPSFSGNQRFNMSCHTPSSRGHSILASPNSTLNDTLLPEMEPILPDLCIEQLWTETSAISREKGCQASKVFITSDLCENRYLCFLVESHQQLRCVKFLESNETSQLIFATVSTIAAKDAAPLEDIHTMLVLEANGSLVLYTGVTRVSKVFVPGLLSPTFSVPNQPPILSTPLENISTPARATVQHMSRLEEVGMPSPVLEMRSSLQHNESSWLEESPFQQTAPRIQTLRDPVCNRVTLELSSGSMLRITIPEVATSELVKRCLQAIRFILPKDTAMKVLVKWYNSYNAPGGPSMHLEWNLFVTCLMTMMGYNTERLTWTRNLHFEVPLSPVIAAKKARPADTGSDEDWLYLRCSDYHWQVSMRPAVSVQGLEMLFPPAGVDDQESALGLESSALLFPHIPLLFYVLHLLYEDLKLDELQRSGARALVGLLQQLARDLQLEDFIDLYWRDFPSFIKTFKEACVIDQAQMELMTWPVFLNGEAPCVFNWISRCLRGQDVAPFPYLPGICERTKLLVLSYALYVVGDEDACSTNMLKYLCRLSAGQKKNASDPFLRRQSSVKPISSSGCLAEKLVLWLTSAGFTLKDLETVPFGVALPIRDAIYQCREHPCSDWSEEVCALIGRQDLTRQAHKVTLANRKSTVGLNVMLEPPVSSEADEEEDGMTDLHPEVTALIWSQDLRVQEVRRLLQSSRPVRVNVPHLPEISDHEYIEEKENKLLQLCQRTMSLPVGRGLFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTMVDLNSGNIDVPPNMTSWPSFHNGVAAGLKIAPASQVESAWIAYNKPKSAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHSVQVAAVVGIGLVYQGTGHRHNAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRTHAGINREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSVADWLKAPDTMFLLDFIKPEFLLLRTLARCLIMWDEIFPNADWVTSNVPQIIQENIGNQDQTPVSEDLNMETLVQAHDYIIAGGCLAVGFRFAGSANSDAFDCLYKFAKNFMQCLTTSTATVTGQYNLQTCLSMVLLAVSMVMSGSGNLKVLQLCRFLHKRTFGEMNYGFHMAHHMALGLLFLGGGRYTLSTSNSAIAALLCALYPHFPAHSTDNRYHLQALRHLAVLAAEPRLLVPVDVDSLKPCYALLDVTYKETQWYEETTVELMAPTMLPELHLLKRIKVKGPRYWELNIDISKGTQHLQSILSRDGVLYVKLRAGQLPYKDDPQGWKSLLAPNLNNRSAEVRTFKPEAISTFTSDAALVSFAEHFCRASENMKHRQETVALLSAILYECVTQENPEMLPTYISIEQAVRTLERADLSETFSLWQLKLVLELCESRLARALLLSSEFLPIMKSSADRALDHWLCDNSNVLKEYLRRQTMNGDTDVNMLACFLVYHSIPSLKNMLASDLEGCSSFPELLLKSSQLGVPIRALLRLSPLLLQNHDSGV

Target ORF information:

RefSeq Version	XM_005157067.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157067.2

ORF Insert Sequence:

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGCAGG 
GAACCTCTCC CGACTTTGTT CAGCATGCTG CATCCATTAG ATGAAATCGC TCCTGTTGTT 
TGTCTGTTTG AAACCCGGCT CCAGTATGTT TCAGACAGCG CTCTGAGAGT GGTGTTCACC 
TGCCTCAAAC CCTCCATTAT CATGACCTAC GACACACAGC AGTGCACACA CACCGTCTGG 
AATCTGCGCA AAGTCACATC TGAGGAGCAG AACACTGTGT TAAAGTTTCC AGATCAGTCT 
GGACCGGCTC ACACTCCTAA TGCGGCTCCC AGTTTCCTCA GCACACACCT GAGAAACGTG 
AACCGGCTGG ACTCTCCGGC TTCACCTTTA CACTGCCATG CTCTTCACAA CCAAAGCCGA 
ATCCCAGCCT CCCCGGCCCT GCACTCTCGG GTTCACTCGC CCAGCATTTC CAACATGGCT 
GCTCTCAGTC GTGCTCATTC CCCTGGCATC GCCGTGCCCT CGTTCTCTGG GAATCAGCGT 
TTCAACATGT CATGCCACAC GCCATCTTCG CGTGGCCACA GCATCCTGGC TTCACCCAAC 
AGCACCCTGA ATGACACCCT GCTGCCTGAG ATGGAGCCCA TCTTACCAGA CCTCTGCATC 
GAGCAGCTGT GGACGGAAAC CAGCGCCATC TCCAGGGAGA AAGGCTGCCA GGCGTCTAAA 
GTCTTCATCA CCTCTGATCT GTGTGAAAAC CGGTACCTGT GCTTCCTGGT CGAGTCCCAT 
CAGCAGCTCA GGTGTGTAAA ATTTCTTGAG AGCAATGAAA CGTCTCAACT GATCTTTGCA 
ACGGTTTCCA CCATCGCTGC AAAAGATGCC GCTCCTCTAG AGGACATTCA CACAATGCTG 
GTGCTGGAGG CCAATGGGAG TCTGGTGTTG TACACAGGAG TCACCAGGGT CAGCAAGGTG 
TTTGTCCCTG GCCTTCTGTC TCCTACATTC AGCGTGCCGA ACCAGCCGCC AATATTGAGC 
ACACCTCTGG AAAACATCAG CACTCCGGCC CGAGCCACAG TTCAGCATAT GAGCCGTCTG 
GAGGAGGTTG GCATGCCGTC TCCTGTGCTG GAGATGCGCA GCTCGTTGCA GCACAATGAG 
TCCTCATGGC TGGAAGAGTC TCCATTTCAG CAAACTGCTC CTCGTATTCA GACCCTCCGA 
GACCCCGTCT GCAACAGAGT CACACTGGAA TTGAGTTCCG GTTCCATGTT GAGAATCACC 
ATTCCCGAGG TGGCCACTTC AGAGCTGGTC AAACGCTGCC TGCAGGCCAT CCGATTTATT 
TTACCCAAGG ACACTGCCAT GAAAGTGCTG GTCAAGTGGT ACAACAGCTA CAACGCCCCC 
GGAGGTCCCA GCATGCACCT GGAGTGGAAC CTGTTCGTCA CCTGCCTAAT GACAATGATG 
GGTTACAACA CAGAGCGGCT GACGTGGACA CGCAACCTTC ATTTTGAAGT GCCTCTTTCA 
CCAGTAATCG CTGCTAAGAA AGCCCGTCCT GCCGACACTG GGTCAGATGA GGATTGGCTG 
TATCTCCGCT GCTCAGACTA TCATTGGCAG GTGTCCATGC GGCCTGCGGT GTCTGTTCAG 
GGTCTGGAGA TGCTGTTTCC CCCTGCTGGA GTGGATGATC AGGAGTCTGC TCTGGGTTTG 
GAGTCGTCCG CTCTCCTTTT CCCACACATC CCGCTGCTCT TTTATGTCCT GCATCTGCTG 
TATGAGGACC TGAAGCTGGA CGAGTTGCAG AGGAGTGGTG CCAGGGCTCT GGTTGGCCTG 
CTGCAACAGC TTGCCAGGGA CCTGCAGCTG GAGGATTTCA TTGATCTGTA CTGGCGTGAT 
TTCCCCTCAT TCATCAAAAC GTTCAAGGAG GCCTGTGTGA TTGATCAAGC TCAGATGGAG 
CTGATGACGT GGCCGGTGTT TCTGAATGGA GAAGCTCCGT GTGTGTTCAA CTGGATCAGT 
CGCTGTCTGA GAGGACAAGA TGTTGCGCCG TTTCCATATC TGCCTGGCAT CTGTGAGAGA 
ACCAAACTAC TGGTGCTGAG TTATGCCCTG TACGTGGTGG GAGATGAAGA CGCCTGCTCC 
ACCAACATGC TCAAATACCT TTGCAGACTG TCAGCAGGGC AAAAGAAGAA TGCAAGTGAT 
CCATTTCTGA GAAGGCAGAG CAGTGTGAAG CCCATATCTT CATCCGGATG CCTGGCTGAG 
AAGCTGGTGC TGTGGTTAAC CTCCGCAGGA TTTACACTGA AGGATCTCGA GACGGTGCCG 
TTCGGTGTGG CTCTGCCAAT TAGAGACGCC ATCTATCAGT GCAGAGAACA CCCCTGCTCT 
GATTGGTCCG AAGAGGTCTG CGCTCTGATT GGCCGACAGG ACCTGACCAG ACAAGCTCAC 
AAAGTCACTC TAGCCAATCG TAAATCGACG GTGGGTCTTA ACGTGATGCT GGAGCCGCCG 
GTGAGCAGCG AAGCCGATGA GGAAGAGGAC GGCATGACGG ATCTTCACCC CGAGGTGACC 
GCGCTCATCT GGAGTCAAGA TCTCAGGGTG CAGGAGGTGC GGCGGCTGCT CCAGAGCTCC 
AGACCCGTCC GTGTTAATGT GCCACATCTG CCGGAGATCA GTGACCACGA GTACATTGAG 
GAGAAGGAGA ACAAGCTCCT CCAGCTGTGC CAGCGCACCA TGTCTCTCCC AGTGGGACGA 
GGGCTTTTTA CTCTCTTCTC ATACCACCCT GTGCCAACCG AACCACTGCC TGTGCCCAAA 
CTCAACCTGA CTGGCCGTGC TCCTCCCAGG AACACCATGG TGGACCTGAA CAGTGGAAAC 
ATCGACGTAC CACCCAACAT GACCAGCTGG CCCAGCTTTC ACAACGGCGT GGCGGCAGGC 
CTGAAAATCG CTCCTGCTTC TCAAGTTGAG TCCGCTTGGA TCGCCTACAA CAAACCCAAA 
AGTGCCGAAC TGGCCAATGA GTATGCTGGT TTCCTGATGG CACTGGGCCT CAATGGTCAC 
CTCACCAAAC TAGCCACTCT CAACATCCAT GACTACCTCA CCAAGGGTCA TGAAATGACG 
AGTATCGGGC TGCTTTTGGG TGTTTCTGCT GCTAAACTGG GCACCATGGA CATGTCCATC 
ACTCGTCTGC TGAGCATCCA CATCCCTGCA TTGCTGCCGC CGACCTCCAC CGAGCTGGAC 
GTCCCACACA GTGTGCAGGT GGCTGCTGTG GTCGGCATCG GCCTGGTCTA TCAGGGCACC 
GGACACCGGC ACAACGCAGA GGTGCTGCTG GCAGAGATCG GTCGTCCGCC GGGTCCAGAG 
ATGGAATACT GCACGGACAG AGAGTCATAT TCTCTAGCTG CAGGACTGGC ACTCGGCATG 
GTCTGCCTCG GGCACGGCAG TAACCTGATC GGGATGAGTG ACCTGAACGT GCCCGAGCAG 
CTCTATCAGT ACATGGTGGG AGGTCATCGC CGCACTCATG CTGGCATCAA CAGAGAGAAA 
CACAAATCTC CCAGCTATCA GATCAAGGAA GGTGACACCA TCAATGTAGA CGTGACTTGT 
CCTGGGGCTA CACTTGCTCT GGCCATGATT TACCTCAAAA CAAATAATAG GTCCGTTGCT 
GATTGGCTGA AAGCCCCAGA CACCATGTTC CTGCTGGACT TCATCAAGCC TGAGTTCCTG 
CTGCTGAGGA CGCTGGCCAG ATGCCTTATC ATGTGGGACG AGATCTTTCC CAATGCCGAC 
TGGGTGACCA GCAATGTTCC TCAGATCATC CAAGAAAACA TCGGAAACCA GGACCAGACG 
CCCGTCTCGG AGGACCTCAA TATGGAGACT TTGGTTCAGG CTCATGACTA TATAATCGCC 
GGAGGCTGTT TGGCTGTGGG ATTTCGGTTT GCTGGCTCTG CTAATTCAGA CGCCTTTGAC 
TGCTTATACA AATTTGCCAA AAACTTCATG CAGTGTTTGA CCACCTCTAC TGCTACAGTG 
ACAGGCCAGT ATAACCTGCA GACGTGTTTG AGCATGGTTC TTCTGGCAGT TTCTATGGTC 
ATGTCCGGCT CAGGGAACCT GAAAGTGCTG CAGTTGTGCC GCTTTCTCCA CAAACGCACC 
TTTGGAGAGA TGAACTATGG GTTCCACATG GCTCATCACA TGGCCCTGGG TCTGCTCTTC 
CTGGGCGGAG GGAGATACAC CCTCAGCACC TCCAACTCGG CCATCGCCGC GCTGCTCTGT 
GCACTTTATC CTCATTTTCC AGCTCACAGC ACAGACAACA GGTATCACCT CCAGGCGTTG 
CGTCATCTGG CTGTCCTTGC GGCAGAACCT CGTCTGCTGG TGCCTGTGGA TGTGGACTCT 
CTCAAACCCT GCTACGCTCT GCTGGATGTC ACCTACAAGG AGACGCAGTG GTACGAAGAG 
ACCACCGTGG AGTTGATGGC TCCCACAATG CTGCCCGAAC TCCACCTGCT GAAGCGGATT 
AAGGTGAAAG GTCCACGATA CTGGGAGCTG AACATCGACA TCAGTAAAGG AACCCAGCAT 
CTGCAATCCA TCCTGTCCAG AGACGGTGTC CTGTATGTAA AGTTGCGGGC CGGTCAGCTT 
CCCTACAAGG ACGATCCTCA AGGCTGGAAG AGTTTACTGG CCCCGAACCT CAACAACAGA 
AGCGCAGAAG TTCGCACGTT TAAGCCCGAG GCCATCTCAA CCTTCACCTC AGACGCCGCT 
TTGGTCTCCT TCGCTGAGCA TTTCTGCAGA GCCTCGGAAA ACATGAAGCA TAGGCAGGAG 
ACTGTGGCTT TACTGTCCGC CATACTGTAC GAGTGCGTGA CGCAAGAAAA CCCAGAAATG 
CTGCCAACGT ACATCTCCAT TGAGCAGGCG GTGCGGACTC TGGAGCGGGC CGATCTGAGC 
GAGACATTCA GCCTGTGGCA GCTGAAGCTG GTGCTGGAGT TGTGTGAGAG TCGATTGGCT 
CGAGCTCTGC TGCTCAGCTC GGAGTTTCTG CCTATCATGA AGAGCTCAGC AGATCGAGCT 
CTGGATCACT GGCTCTGTGA CAATAGTAAT GTGCTGAAGG AGTACCTAAG ACGACAGACG 
ATGAACGGCG ACACTGATGT CAACATGCTG GCCTGCTTTC TGGTCTATCA TTCAATCCCC 
AGCCTCAAAA ACATGCTGGC GTCCGACCTG GAAGGATGTT CCAGTTTTCC AGAGCTGCTC 
CTCAAGAGCA GTCAGTTGGG TGTTCCCATT CGAGCTCTTC TACGATTGAG TCCTTTACTC 
CTGCAAAATC ACGACTCTGG CGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANAPC1 ( XM_005157067.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157124.1 Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X3, mRNA.

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CloneID	ODa19123
Clone ID Related Accession (Same CDS sequence)	XM_005157067.2 , XM_005157067.3
Accession Version	XM_005157067.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	anaphase-promoting complex subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157067.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGCAGG 
GAACCTCTCC CGACTTTGTT CAGCATGCTG CATCCATTAG ATGAAATCGC TCCTGTTGTT 
TGTCTGTTTG AAACCCGGCT CCAGTATGTT TCAGACAGCG CTCTGAGAGT GGTGTTCACC 
TGCCTCAAAC CCTCCATTAT CATGACCTAC GACACACAGC AGTGCACACA CACCGTCTGG 
AATCTGCGCA AAGTCACATC TGAGGAGCAG AACACTGTGT TAAAGTTTCC AGATCAGTCT 
GGACCGGCTC ACACTCCTAA TGCGGCTCCC AGTTTCCTCA GCACACACCT GAGAAACGTG 
AACCGGCTGG ACTCTCCGGC TTCACCTTTA CACTGCCATG CTCTTCACAA CCAAAGCCGA 
ATCCCAGCCT CCCCGGCCCT GCACTCTCGG GTTCACTCGC CCAGCATTTC CAACATGGCT 
GCTCTCAGTC GTGCTCATTC CCCTGGCATC GCCGTGCCCT CGTTCTCTGG GAATCAGCGT 
TTCAACATGT CATGCCACAC GCCATCTTCG CGTGGCCACA GCATCCTGGC TTCACCCAAC 
AGCACCCTGA ATGACACCCT GCTGCCTGAG ATGGAGCCCA TCTTACCAGA CCTCTGCATC 
GAGCAGCTGT GGACGGAAAC CAGCGCCATC TCCAGGGAGA AAGGCTGCCA GGCGTCTAAA 
GTCTTCATCA CCTCTGATCT GTGTGAAAAC CGGTACCTGT GCTTCCTGGT CGAGTCCCAT 
CAGCAGCTCA GGTGTGTAAA ATTTCTTGAG AGCAATGAAA CGTCTCAACT GATCTTTGCA 
ACGGTTTCCA CCATCGCTGC AAAAGATGCC GCTCCTCTAG AGGACATTCA CACAATGCTG 
GTGCTGGAGG CCAATGGGAG TCTGGTGTTG TACACAGGAG TCACCAGGGT CAGCAAGGTG 
TTTGTCCCTG GCCTTCTGTC TCCTACATTC AGCGTGCCGA ACCAGCCGCC AATATTGAGC 
ACACCTCTGG AAAACATCAG CACTCCGGCC CGAGCCACAG TTCAGCATAT GAGCCGTCTG 
GAGGAGGTTG GCATGCCGTC TCCTGTGCTG GAGATGCGCA GCTCGTTGCA GCACAATGAG 
TCCTCATGGC TGGAAGAGTC TCCATTTCAG CAAACTGCTC CTCGTATTCA GACCCTCCGA 
GACCCCGTCT GCAACAGAGT CACACTGGAA TTGAGTTCCG GTTCCATGTT GAGAATCACC 
ATTCCCGAGG TGGCCACTTC AGAGCTGGTC AAACGCTGCC TGCAGGCCAT CCGATTTATT 
TTACCCAAGG ACACTGCCAT GAAAGTGCTG GTCAAGTGGT ACAACAGCTA CAACGCCCCC 
GGAGGTCCCA GCATGCACCT GGAGTGGAAC CTGTTCGTCA CCTGCCTAAT GACAATGATG 
GGTTACAACA CAGAGCGGCT GACGTGGACA CGCAACCTTC ATTTTGAAGT GCCTCTTTCA 
CCAGTAATCG CTGCTAAGAA AGCCCGTCCT GCCGACACTG GGTCAGATGA GGATTGGCTG 
TATCTCCGCT GCTCAGACTA TCATTGGCAG GTGTCCATGC GGCCTGCGGT GTCTGTTCAG 
GGTCTGGAGA TGCTGTTTCC CCCTGCTGGA GTGGATGATC AGGAGTCTGC TCTGGGTTTG 
GAGTCGTCCG CTCTCCTTTT CCCACACATC CCGCTGCTCT TTTATGTCCT GCATCTGCTG 
TATGAGGACC TGAAGCTGGA CGAGTTGCAG AGGAGTGGTG CCAGGGCTCT GGTTGGCCTG 
CTGCAACAGC TTGCCAGGGA CCTGCAGCTG GAGGATTTCA TTGATCTGTA CTGGCGTGAT 
TTCCCCTCAT TCATCAAAAC GTTCAAGGAG GCCTGTGTGA TTGATCAAGC TCAGATGGAG 
CTGATGACGT GGCCGGTGTT TCTGAATGGA GAAGCTCCGT GTGTGTTCAA CTGGATCAGT 
CGCTGTCTGA GAGGACAAGA TGTTGCGCCG TTTCCATATC TGCCTGGCAT CTGTGAGAGA 
ACCAAACTAC TGGTGCTGAG TTATGCCCTG TACGTGGTGG GAGATGAAGA CGCCTGCTCC 
ACCAACATGC TCAAATACCT TTGCAGACTG TCAGCAGGGC AAAAGAAGAA TGCAAGTGAT 
CCATTTCTGA GAAGGCAGAG CAGTGTGAAG CCCATATCTT CATCCGGATG CCTGGCTGAG 
AAGCTGGTGC TGTGGTTAAC CTCCGCAGGA TTTACACTGA AGGATCTCGA GACGGTGCCG 
TTCGGTGTGG CTCTGCCAAT TAGAGACGCC ATCTATCAGT GCAGAGAACA CCCCTGCTCT 
GATTGGTCCG AAGAGGTCTG CGCTCTGATT GGCCGACAGG ACCTGACCAG ACAAGCTCAC 
AAAGTCACTC TAGCCAATCG TAAATCGACG GTGGGTCTTA ACGTGATGCT GGAGCCGCCG 
GTGAGCAGCG AAGCCGATGA GGAAGAGGAC GGCATGACGG ATCTTCACCC CGAGGTGACC 
GCGCTCATCT GGAGTCAAGA TCTCAGGGTG CAGGAGGTGC GGCGGCTGCT CCAGAGCTCC 
AGACCCGTCC GTGTTAATGT GCCACATCTG CCGGAGATCA GTGACCACGA GTACATTGAG 
GAGAAGGAGA ACAAGCTCCT CCAGCTGTGC CAGCGCACCA TGTCTCTCCC AGTGGGACGA 
GGGCTTTTTA CTCTCTTCTC ATACCACCCT GTGCCAACCG AACCACTGCC TGTGCCCAAA 
CTCAACCTGA CTGGCCGTGC TCCTCCCAGG AACACCATGG TGGACCTGAA CAGTGGAAAC 
ATCGACGTAC CACCCAACAT GACCAGCTGG CCCAGCTTTC ACAACGGCGT GGCGGCAGGC 
CTGAAAATCG CTCCTGCTTC TCAAGTTGAG TCCGCTTGGA TCGCCTACAA CAAACCCAAA 
AGTGCCGAAC TGGCCAATGA GTATGCTGGT TTCCTGATGG CACTGGGCCT CAATGGTCAC 
CTCACCAAAC TAGCCACTCT CAACATCCAT GACTACCTCA CCAAGGGTCA TGAAATGACG 
AGTATCGGGC TGCTTTTGGG TGTTTCTGCT GCTAAACTGG GCACCATGGA CATGTCCATC 
ACTCGTCTGC TGAGCATCCA CATCCCTGCA TTGCTGCCGC CGACCTCCAC CGAGCTGGAC 
GTCCCACACA GTGTGCAGGT GGCTGCTGTG GTCGGCATCG GCCTGGTCTA TCAGGGCACC 
GGACACCGGC ACAACGCAGA GGTGCTGCTG GCAGAGATCG GTCGTCCGCC GGGTCCAGAG 
ATGGAATACT GCACGGACAG AGAGTCATAT TCTCTAGCTG CAGGACTGGC ACTCGGCATG 
GTCTGCCTCG GGCACGGCAG TAACCTGATC GGGATGAGTG ACCTGAACGT GCCCGAGCAG 
CTCTATCAGT ACATGGTGGG AGGTCATCGC CGCACTCATG CTGGCATCAA CAGAGAGAAA 
CACAAATCTC CCAGCTATCA GATCAAGGAA GGTGACACCA TCAATGTAGA CGTGACTTGT 
CCTGGGGCTA CACTTGCTCT GGCCATGATT TACCTCAAAA CAAATAATAG GTCCGTTGCT 
GATTGGCTGA AAGCCCCAGA CACCATGTTC CTGCTGGACT TCATCAAGCC TGAGTTCCTG 
CTGCTGAGGA CGCTGGCCAG ATGCCTTATC ATGTGGGACG AGATCTTTCC CAATGCCGAC 
TGGGTGACCA GCAATGTTCC TCAGATCATC CAAGAAAACA TCGGAAACCA GGACCAGACG 
CCCGTCTCGG AGGACCTCAA TATGGAGACT TTGGTTCAGG CTCATGACTA TATAATCGCC 
GGAGGCTGTT TGGCTGTGGG ATTTCGGTTT GCTGGCTCTG CTAATTCAGA CGCCTTTGAC 
TGCTTATACA AATTTGCCAA AAACTTCATG CAGTGTTTGA CCACCTCTAC TGCTACAGTG 
ACAGGCCAGT ATAACCTGCA GACGTGTTTG AGCATGGTTC TTCTGGCAGT TTCTATGGTC 
ATGTCCGGCT CAGGGAACCT GAAAGTGCTG CAGTTGTGCC GCTTTCTCCA CAAACGCACC 
TTTGGAGAGA TGAACTATGG GTTCCACATG GCTCATCACA TGGCCCTGGG TCTGCTCTTC 
CTGGGCGGAG GGAGATACAC CCTCAGCACC TCCAACTCGG CCATCGCCGC GCTGCTCTGT 
GCACTTTATC CTCATTTTCC AGCTCACAGC ACAGACAACA GGTATCACCT CCAGGCGTTG 
CGTCATCTGG CTGTCCTTGC GGCAGAACCT CGTCTGCTGG TGCCTGTGGA TGTGGACTCT 
CTCAAACCCT GCTACGCTCT GCTGGATGTC ACCTACAAGG AGACGCAGTG GTACGAAGAG 
ACCACCGTGG AGTTGATGGC TCCCACAATG CTGCCCGAAC TCCACCTGCT GAAGCGGATT 
AAGGTGAAAG GTCCACGATA CTGGGAGCTG AACATCGACA TCAGTAAAGG AACCCAGCAT 
CTGCAATCCA TCCTGTCCAG AGACGGTGTC CTGTATGTAA AGTTGCGGGC CGGTCAGCTT 
CCCTACAAGG ACGATCCTCA AGGCTGGAAG AGTTTACTGG CCCCGAACCT CAACAACAGA 
AGCGCAGAAG TTCGCACGTT TAAGCCCGAG GCCATCTCAA CCTTCACCTC AGACGCCGCT 
TTGGTCTCCT TCGCTGAGCA TTTCTGCAGA GCCTCGGAAA ACATGAAGCA TAGGCAGGAG 
ACTGTGGCTT TACTGTCCGC CATACTGTAC GAGTGCGTGA CGCAAGAAAA CCCAGAAATG 
CTGCCAACGT ACATCTCCAT TGAGCAGGCG GTGCGGACTC TGGAGCGGGC CGATCTGAGC 
GAGACATTCA GCCTGTGGCA GCTGAAGCTG GTGCTGGAGT TGTGTGAGAG TCGATTGGCT 
CGAGCTCTGC TGCTCAGCTC GGAGTTTCTG CCTATCATGA AGAGCTCAGC AGATCGAGCT 
CTGGATCACT GGCTCTGTGA CAATAGTAAT GTGCTGAAGG AGTACCTAAG ACGACAGACG 
ATGAACGGCG ACACTGATGT CAACATGCTG GCCTGCTTTC TGGTCTATCA TTCAATCCCC 
AGCCTCAAAA ACATGCTGGC GTCCGACCTG GAAGGATGTT CCAGTTTTCC AGAGCTGCTC 
CTCAAGAGCA GTCAGTTGGG TGTTCCCATT CGAGCTCTTC TACGATTGAG TCCTTTACTC 
CTGCAAAATC ACGACTCTGG CGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157124.1
CDS	228..6014
Translation	MSHVYEERATMIAAGELQSFVPFGREHCKNHPNAVNLQLRQLQPASELWSSDGAAGLVGSLQEVTLQERDKERWQLRKGTAPDDVEYDEELYVAGNMVIWSRGSKNQASCVYKAFTVDSPVQQALWCNFTVPQAKKDVKDVVEQTVCIVQSTCVNVHSMSGKDFISPLPFQVSALWPTKFGLLLERKNITVDGHMSPISREPLPTLFSMLHPLDEIAPVVCLFETRLQYVSDSALRVVFTCLKPSIIMTYDTQQCTHTVWNLRKVTSEEQNTVLKFPDQSGPAHTPNAAPSFLSTHLRNVNRLDSPASPLHCHALHNQSRIPASPALHSRVHSPSISNMAALSRAHSPGIAVPSFSGNQRFNMSCHTPSSRGHSILASPNSTLNDTLLPEMEPILPDLCIEQLWTETSAISREKGCQASKVFITSDLCENRYLCFLVESHQQLRCVKFLESNETSQLIFATVSTIAAKDAAPLEDIHTMLVLEANGSLVLYTGVTRVSKVFVPGLLSPTFSVPNQPPILSTPLENISTPARATVQHMSRLEEVGMPSPVLEMRSSLQHNESSWLEESPFQQTAPRIQTLRDPVCNRVTLELSSGSMLRITIPEVATSELVKRCLQAIRFILPKDTAMKVLVKWYNSYNAPGGPSMHLEWNLFVTCLMTMMGYNTERLTWTRNLHFEVPLSPVIAAKKARPADTGSDEDWLYLRCSDYHWQVSMRPAVSVQGLEMLFPPAGVDDQESALGLESSALLFPHIPLLFYVLHLLYEDLKLDELQRSGARALVGLLQQLARDLQLEDFIDLYWRDFPSFIKTFKEACVIDQAQMELMTWPVFLNGEAPCVFNWISRCLRGQDVAPFPYLPGICERTKLLVLSYALYVVGDEDACSTNMLKYLCRLSAGQKKNASDPFLRRQSSVKPISSSGCLAEKLVLWLTSAGFTLKDLETVPFGVALPIRDAIYQCREHPCSDWSEEVCALIGRQDLTRQAHKVTLANRKSTVGLNVMLEPPVSSEADEEEDGMTDLHPEVTALIWSQDLRVQEVRRLLQSSRPVRVNVPHLPEISDHEYIEEKENKLLQLCQRTMSLPVGRGLFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTMVDLNSGNIDVPPNMTSWPSFHNGVAAGLKIAPASQVESAWIAYNKPKSAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHSVQVAAVVGIGLVYQGTGHRHNAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMVGGHRRTHAGINREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSVADWLKAPDTMFLLDFIKPEFLLLRTLARCLIMWDEIFPNADWVTSNVPQIIQENIGNQDQTPVSEDLNMETLVQAHDYIIAGGCLAVGFRFAGSANSDAFDCLYKFAKNFMQCLTTSTATVTGQYNLQTCLSMVLLAVSMVMSGSGNLKVLQLCRFLHKRTFGEMNYGFHMAHHMALGLLFLGGGRYTLSTSNSAIAALLCALYPHFPAHSTDNRYHLQALRHLAVLAAEPRLLVPVDVDSLKPCYALLDVTYKETQWYEETTVELMAPTMLPELHLLKRIKVKGPRYWELNIDISKGTQHLQSILSRDGVLYVKLRAGQLPYKDDPQGWKSLLAPNLNNRSAEVRTFKPEAISTFTSDAALVSFAEHFCRASENMKHRQETVALLSAILYECVTQENPEMLPTYISIEQAVRTLERADLSETFSLWQLKLVLELCESRLARALLLSSEFLPIMKSSADRALDHWLCDNSNVLKEYLRRQTMNGDTDVNMLACFLVYHSIPSLKNMLASDLEGCSSFPELLLKSSQLGVPIRALLRLSPLLLQNHDSGV

Target ORF information:

RefSeq Version	XM_005157067.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anaphase promoting complex subunit 1 (anapc1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157067.3

ORF Insert Sequence:

ATGTCGCACG TCTATGAAGA GAGAGCCACA ATGATAGCCG CAGGCGAATT GCAGTCGTTT 
GTCCCATTCG GTCGTGAGCA CTGCAAAAAT CACCCGAATG CTGTGAATCT CCAGCTGCGG 
CAGCTCCAGC CGGCATCTGA GCTCTGGTCC TCCGACGGGG CAGCCGGGCT TGTGGGATCT 
CTTCAGGAGG TAACTCTACA GGAGCGTGAC AAGGAGCGCT GGCAGCTGAG GAAGGGAACG 
GCTCCTGATG ATGTTGAATA TGACGAGGAG CTGTATGTGG CTGGAAACAT GGTCATCTGG 
AGTCGTGGCA GTAAGAATCA AGCCTCATGT GTGTACAAGG CTTTCACAGT CGACAGCCCA 
GTTCAGCAGG CTCTTTGGTG TAATTTCACT GTTCCTCAGG CTAAAAAAGA TGTAAAGGAC 
GTTGTGGAGC AGACGGTGTG TATTGTCCAG AGCACCTGTG TGAACGTCCA CAGCATGAGC 
GGGAAAGACT TCATATCTCC ACTTCCCTTC CAGGTTTCTG CACTCTGGCC AACAAAGTTT 
GGCCTACTTT TGGAGCGCAA AAATATAACA GTTGATGGCC ACATGAGCCC CATCAGCAGG 
GAACCTCTCC CGACTTTGTT CAGCATGCTG CATCCATTAG ATGAAATCGC TCCTGTTGTT 
TGTCTGTTTG AAACCCGGCT CCAGTATGTT TCAGACAGCG CTCTGAGAGT GGTGTTCACC 
TGCCTCAAAC CCTCCATTAT CATGACCTAC GACACACAGC AGTGCACACA CACCGTCTGG 
AATCTGCGCA AAGTCACATC TGAGGAGCAG AACACTGTGT TAAAGTTTCC AGATCAGTCT 
GGACCGGCTC ACACTCCTAA TGCGGCTCCC AGTTTCCTCA GCACACACCT GAGAAACGTG 
AACCGGCTGG ACTCTCCGGC TTCACCTTTA CACTGCCATG CTCTTCACAA CCAAAGCCGA 
ATCCCAGCCT CCCCGGCCCT GCACTCTCGG GTTCACTCGC CCAGCATTTC CAACATGGCT 
GCTCTCAGTC GTGCTCATTC CCCTGGCATC GCCGTGCCCT CGTTCTCTGG GAATCAGCGT 
TTCAACATGT CATGCCACAC GCCATCTTCG CGTGGCCACA GCATCCTGGC TTCACCCAAC 
AGCACCCTGA ATGACACCCT GCTGCCTGAG ATGGAGCCCA TCTTACCAGA CCTCTGCATC 
GAGCAGCTGT GGACGGAAAC CAGCGCCATC TCCAGGGAGA AAGGCTGCCA GGCGTCTAAA 
GTCTTCATCA CCTCTGATCT GTGTGAAAAC CGGTACCTGT GCTTCCTGGT CGAGTCCCAT 
CAGCAGCTCA GGTGTGTAAA ATTTCTTGAG AGCAATGAAA CGTCTCAACT GATCTTTGCA 
ACGGTTTCCA CCATCGCTGC AAAAGATGCC GCTCCTCTAG AGGACATTCA CACAATGCTG 
GTGCTGGAGG CCAATGGGAG TCTGGTGTTG TACACAGGAG TCACCAGGGT CAGCAAGGTG 
TTTGTCCCTG GCCTTCTGTC TCCTACATTC AGCGTGCCGA ACCAGCCGCC AATATTGAGC 
ACACCTCTGG AAAACATCAG CACTCCGGCC CGAGCCACAG TTCAGCATAT GAGCCGTCTG 
GAGGAGGTTG GCATGCCGTC TCCTGTGCTG GAGATGCGCA GCTCGTTGCA GCACAATGAG 
TCCTCATGGC TGGAAGAGTC TCCATTTCAG CAAACTGCTC CTCGTATTCA GACCCTCCGA 
GACCCCGTCT GCAACAGAGT CACACTGGAA TTGAGTTCCG GTTCCATGTT GAGAATCACC 
ATTCCCGAGG TGGCCACTTC AGAGCTGGTC AAACGCTGCC TGCAGGCCAT CCGATTTATT 
TTACCCAAGG ACACTGCCAT GAAAGTGCTG GTCAAGTGGT ACAACAGCTA CAACGCCCCC 
GGAGGTCCCA GCATGCACCT GGAGTGGAAC CTGTTCGTCA CCTGCCTAAT GACAATGATG 
GGTTACAACA CAGAGCGGCT GACGTGGACA CGCAACCTTC ATTTTGAAGT GCCTCTTTCA 
CCAGTAATCG CTGCTAAGAA AGCCCGTCCT GCCGACACTG GGTCAGATGA GGATTGGCTG 
TATCTCCGCT GCTCAGACTA TCATTGGCAG GTGTCCATGC GGCCTGCGGT GTCTGTTCAG 
GGTCTGGAGA TGCTGTTTCC CCCTGCTGGA GTGGATGATC AGGAGTCTGC TCTGGGTTTG 
GAGTCGTCCG CTCTCCTTTT CCCACACATC CCGCTGCTCT TTTATGTCCT GCATCTGCTG 
TATGAGGACC TGAAGCTGGA CGAGTTGCAG AGGAGTGGTG CCAGGGCTCT GGTTGGCCTG 
CTGCAACAGC TTGCCAGGGA CCTGCAGCTG GAGGATTTCA TTGATCTGTA CTGGCGTGAT 
TTCCCCTCAT TCATCAAAAC GTTCAAGGAG GCCTGTGTGA TTGATCAAGC TCAGATGGAG 
CTGATGACGT GGCCGGTGTT TCTGAATGGA GAAGCTCCGT GTGTGTTCAA CTGGATCAGT 
CGCTGTCTGA GAGGACAAGA TGTTGCGCCG TTTCCATATC TGCCTGGCAT CTGTGAGAGA 
ACCAAACTAC TGGTGCTGAG TTATGCCCTG TACGTGGTGG GAGATGAAGA CGCCTGCTCC 
ACCAACATGC TCAAATACCT TTGCAGACTG TCAGCAGGGC AAAAGAAGAA TGCAAGTGAT 
CCATTTCTGA GAAGGCAGAG CAGTGTGAAG CCCATATCTT CATCCGGATG CCTGGCTGAG 
AAGCTGGTGC TGTGGTTAAC CTCCGCAGGA TTTACACTGA AGGATCTCGA GACGGTGCCG 
TTCGGTGTGG CTCTGCCAAT TAGAGACGCC ATCTATCAGT GCAGAGAACA CCCCTGCTCT 
GATTGGTCCG AAGAGGTCTG CGCTCTGATT GGCCGACAGG ACCTGACCAG ACAAGCTCAC 
AAAGTCACTC TAGCCAATCG TAAATCGACG GTGGGTCTTA ACGTGATGCT GGAGCCGCCG 
GTGAGCAGCG AAGCCGATGA GGAAGAGGAC GGCATGACGG ATCTTCACCC CGAGGTGACC 
GCGCTCATCT GGAGTCAAGA TCTCAGGGTG CAGGAGGTGC GGCGGCTGCT CCAGAGCTCC 
AGACCCGTCC GTGTTAATGT GCCACATCTG CCGGAGATCA GTGACCACGA GTACATTGAG 
GAGAAGGAGA ACAAGCTCCT CCAGCTGTGC CAGCGCACCA TGTCTCTCCC AGTGGGACGA 
GGGCTTTTTA CTCTCTTCTC ATACCACCCT GTGCCAACCG AACCACTGCC TGTGCCCAAA 
CTCAACCTGA CTGGCCGTGC TCCTCCCAGG AACACCATGG TGGACCTGAA CAGTGGAAAC 
ATCGACGTAC CACCCAACAT GACCAGCTGG CCCAGCTTTC ACAACGGCGT GGCGGCAGGC 
CTGAAAATCG CTCCTGCTTC TCAAGTTGAG TCCGCTTGGA TCGCCTACAA CAAACCCAAA 
AGTGCCGAAC TGGCCAATGA GTATGCTGGT TTCCTGATGG CACTGGGCCT CAATGGTCAC 
CTCACCAAAC TAGCCACTCT CAACATCCAT GACTACCTCA CCAAGGGTCA TGAAATGACG 
AGTATCGGGC TGCTTTTGGG TGTTTCTGCT GCTAAACTGG GCACCATGGA CATGTCCATC 
ACTCGTCTGC TGAGCATCCA CATCCCTGCA TTGCTGCCGC CGACCTCCAC CGAGCTGGAC 
GTCCCACACA GTGTGCAGGT GGCTGCTGTG GTCGGCATCG GCCTGGTCTA TCAGGGCACC 
GGACACCGGC ACAACGCAGA GGTGCTGCTG GCAGAGATCG GTCGTCCGCC GGGTCCAGAG 
ATGGAATACT GCACGGACAG AGAGTCATAT TCTCTAGCTG CAGGACTGGC ACTCGGCATG 
GTCTGCCTCG GGCACGGCAG TAACCTGATC GGGATGAGTG ACCTGAACGT GCCCGAGCAG 
CTCTATCAGT ACATGGTGGG AGGTCATCGC CGCACTCATG CTGGCATCAA CAGAGAGAAA 
CACAAATCTC CCAGCTATCA GATCAAGGAA GGTGACACCA TCAATGTAGA CGTGACTTGT 
CCTGGGGCTA CACTTGCTCT GGCCATGATT TACCTCAAAA CAAATAATAG GTCCGTTGCT 
GATTGGCTGA AAGCCCCAGA CACCATGTTC CTGCTGGACT TCATCAAGCC TGAGTTCCTG 
CTGCTGAGGA CGCTGGCCAG ATGCCTTATC ATGTGGGACG AGATCTTTCC CAATGCCGAC 
TGGGTGACCA GCAATGTTCC TCAGATCATC CAAGAAAACA TCGGAAACCA GGACCAGACG 
CCCGTCTCGG AGGACCTCAA TATGGAGACT TTGGTTCAGG CTCATGACTA TATAATCGCC 
GGAGGCTGTT TGGCTGTGGG ATTTCGGTTT GCTGGCTCTG CTAATTCAGA CGCCTTTGAC 
TGCTTATACA AATTTGCCAA AAACTTCATG CAGTGTTTGA CCACCTCTAC TGCTACAGTG 
ACAGGCCAGT ATAACCTGCA GACGTGTTTG AGCATGGTTC TTCTGGCAGT TTCTATGGTC 
ATGTCCGGCT CAGGGAACCT GAAAGTGCTG CAGTTGTGCC GCTTTCTCCA CAAACGCACC 
TTTGGAGAGA TGAACTATGG GTTCCACATG GCTCATCACA TGGCCCTGGG TCTGCTCTTC 
CTGGGCGGAG GGAGATACAC CCTCAGCACC TCCAACTCGG CCATCGCCGC GCTGCTCTGT 
GCACTTTATC CTCATTTTCC AGCTCACAGC ACAGACAACA GGTATCACCT CCAGGCGTTG 
CGTCATCTGG CTGTCCTTGC GGCAGAACCT CGTCTGCTGG TGCCTGTGGA TGTGGACTCT 
CTCAAACCCT GCTACGCTCT GCTGGATGTC ACCTACAAGG AGACGCAGTG GTACGAAGAG 
ACCACCGTGG AGTTGATGGC TCCCACAATG CTGCCCGAAC TCCACCTGCT GAAGCGGATT 
AAGGTGAAAG GTCCACGATA CTGGGAGCTG AACATCGACA TCAGTAAAGG AACCCAGCAT 
CTGCAATCCA TCCTGTCCAG AGACGGTGTC CTGTATGTAA AGTTGCGGGC CGGTCAGCTT 
CCCTACAAGG ACGATCCTCA AGGCTGGAAG AGTTTACTGG CCCCGAACCT CAACAACAGA 
AGCGCAGAAG TTCGCACGTT TAAGCCCGAG GCCATCTCAA CCTTCACCTC AGACGCCGCT 
TTGGTCTCCT TCGCTGAGCA TTTCTGCAGA GCCTCGGAAA ACATGAAGCA TAGGCAGGAG 
ACTGTGGCTT TACTGTCCGC CATACTGTAC GAGTGCGTGA CGCAAGAAAA CCCAGAAATG 
CTGCCAACGT ACATCTCCAT TGAGCAGGCG GTGCGGACTC TGGAGCGGGC CGATCTGAGC 
GAGACATTCA GCCTGTGGCA GCTGAAGCTG GTGCTGGAGT TGTGTGAGAG TCGATTGGCT 
CGAGCTCTGC TGCTCAGCTC GGAGTTTCTG CCTATCATGA AGAGCTCAGC AGATCGAGCT 
CTGGATCACT GGCTCTGTGA CAATAGTAAT GTGCTGAAGG AGTACCTAAG ACGACAGACG 
ATGAACGGCG ACACTGATGT CAACATGCTG GCCTGCTTTC TGGTCTATCA TTCAATCCCC 
AGCCTCAAAA ACATGCTGGC GTCCGACCTG GAAGGATGTT CCAGTTTTCC AGAGCTGCTC 
CTCAAGAGCA GTCAGTTGGG TGTTCCCATT CGAGCTCTTC TACGATTGAG TCCTTTACTC 
CTGCAAAATC ACGACTCTGG CGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ANAPC1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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