mier3b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mier3b
Entrez Gene ID	567247
Full Name	mesoderm induction early response 1, family member 3 b
Synonyms	zgc:162295
General protein information	Preferred Names mesoderm induction early response 1, family member 3 b Names uncharacterized protein LOC567247
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005166879.4	XP_005166936.1	uncharacterized protein LOC567247 isoform X1
XM_005166880.3	XP_005166937.1	uncharacterized protein LOC567247 isoform X2
NM_001083560.1	NP_001077029.1	uncharacterized protein LOC567247
XM_005166879.3	XP_005166936.1	uncharacterized protein LOC567247 isoform X1
XM_005166880.4	XP_005166937.1	uncharacterized protein LOC567247 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	zgc:162295	NP_001077029.1
Homo sapiens (human)	MIER3	NP_689835.3
Macaca mulatta (Rhesus monkey)	LOC711063	XP_001099786.2
Gallus gallus (chicken)	MIER3	XP_424736.3
Canis lupus familiaris (dog)	MIER3	XP_544343.4
Xenopus tropicalis (tropical clawed frog)	mier3	NP_001008086.1
Pan troglodytes (chimpanzee)	MIER3	XP_001142293.2
Mus musculus (house mouse)	Mier3	NP_766181.2
Rattus norvegicus (Norway rat)	Mier3	NP_001161472.1
Danio rerio (zebrafish)	mier3	NP_001025320.2
Bos taurus (cattle)	MIER3	XP_003587569.2

Gene Ontology
Bibliography

Related articles in PubMed

Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development.
Yang H, Fang L, Zhan R, Hegarty JM, Ren J, Hsiai TK, Gleeson JG, Miller YI, Trejo J, Chi NC
Developmental biology404(2)49-60(2015 Aug)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mier3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mier3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa04986	NM_001083560.1 Latest version!	Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa14016	XM_005166879.4 Latest version!	Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa14017	XM_005166880.4 Latest version!	Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa14016	XM_005166879.3	Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa14017	XM_005166880.3	Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mier3b ( NM_001083560.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001077029.1 Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), mRNA.

Price & Availability↑

CloneID	ODa04986
Clone ID Related Accession (Same CDS sequence)	NM_001083560.1
Accession Version	NM_001083560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1485705600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC567247
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from BC134953.1. On Mar 28, 2007 this sequence version replaced XM_690539.2. ##Evidence-Data-START## Transcript exon combination :: BC134953.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA898400 [ECO:0000348] ##Evidence-Data-END##

ATGCTGGCTT CCTTGGGGAG TTCGAGCCCT GTTGGCTCTT TGTCTTCAGA GGATCACGAC 
TTTGATCCAA CCGCTGAGAT GTTGATTCAT GAGTATGATG ATGAGAGAAC ATTGGAGGAG 
GAAGAGCTTT TGGAAGGAGA GAGAAATTTC AGTGCAGAAC TGTCCGACTT GGAAAGGGAG 
GGGAACATGC CGATCGAGGA GCTGTTGGCC ATGTATCGTT ACGAGGCGTC AGTCAGTACC 
GGTGCAGGAT CAAGCATTGA CAGTTCCTCT GTTGAACTAG CAGATGATCT GCCTGACATG 
ACCCTGGATA AGGAGGAGAT TGCTAAAGAT CTCCTGTCAG GAGATTATGA GGAGGAGACC 
CAGTCGTCTG CTGATGATCT GACCCCTTCT GTCACTTCCC ATGAGGCCAC GGATTTCTTC 
CCCAGAACTC TAAGATCCAA TGTAACATAT GACGGTGATA AAGAATCTGA GGGGGAGGAG 
GACGGTGTAA ACAGTGAAGA TTCTAGAAAG GAAATAATGG TGGGATCTCA ATATCAAGCA 
GAAGTCCCAG CAGGGCTCAG CCACTATAAT GATGATGAAA AGGTGTACGA GGAGGAAGAT 
CAGCTGCTGT GGTGTCCTGA CGAACTGTCA GAGTATAAAG TGAAAGACTA CCTCAGAGAA 
GCACTGTCAC AGTCTACAGA TGACCGCACG CATTATTCCA CATCTGAACA CATACGAGAC 
AACGAGCAGG CTTTGTTTGA GCTTTTGAAA TGTAATTATG ACACTCGTGA GGCGCTGGTT 
CGATACTGCA GTAATGTAAA GACCTCAAAA GAAGAATCAC CACCATGGTC AGAGGAGGAA 
TGTAGGAACT TCGAACATGC GCTGCAGATT TATGAGAAAA ACTTCCATCT TATTCAGAAA 
CACAAGGTTC AAACCCGGAC TGTAGCAGAA TGTGTGGCTT TTTACTACAT GTGGAAAAAG 
TCGGAACGTT TTGACTACTT TGCTCAACAG AACCGATTTG GCAAGAAGAA ATACAGCTGT 
TATCCTGGTG TGACGGACCT GATGGATCGG CTGGTAGACG AGGCTGAGGG TCTGGCAGTG 
GACGGCTCTT CACCGGTGTG TTCAGCTGGA GCAGGAGGAA GACTAGAGGC ATCGGCAGAG 
CAACAGCTCG GACTGCTCAA CTCCATCACT GCCAGTGACC TCACAGCTCT TAGTAACAGT 
GTAGCGAATG TGTGCAACGG TCCAGCAGAG ATGAGCTGTT TGGACTCTCC GTTCTTCCCC 
CCTCTGGAGA GTTTGCACCG AGCAGCCCTG AACCATGACG ATCAGCTCAG CTACGGAACG 
AATGGAGACG GCGTCTGCCT CAACATGCTG GATTCAGGCT TTTACCGCTC TGACCTGGGC 
CAGCTCAGCA TGTGCAGCAC CAAAGACTGC GAACGACCCT CCAAGAGGCT CAAGATGGGC 
CTTTCAGAGT CCTTCATCAA CGACGTGGAC TTCGAGGGCA GACGAGCACA TCGCATTACA 
GGCGCCACAA TGGCGGTTTC GGTCACAGAC TTCAGTGGCA TCGGGAGTGA AGCCAACGCT 
TTCATCAGCT CTCATTCACG ACACCACCAA CACACTGCAC TTCAGTCCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077029.1
CDS	84..1697
Translation	MLASLGSSSPVGSLSSEDHDFDPTAEMLIHEYDDERTLEEEELLEGERNFSAELSDLEREGNMPIEELLAMYRYEASVSTGAGSSIDSSSVELADDLPDMTLDKEEIAKDLLSGDYEEETQSSADDLTPSVTSHEATDFFPRTLRSNVTYDGDKESEGEEDGVNSEDSRKEIMVGSQYQAEVPAGLSHYNDDEKVYEEEDQLLWCPDELSEYKVKDYLREALSQSTDDRTHYSTSEHIRDNEQALFELLKCNYDTREALVRYCSNVKTSKEESPPWSEEECRNFEHALQIYEKNFHLIQKHKVQTRTVAECVAFYYMWKKSERFDYFAQQNRFGKKKYSCYPGVTDLMDRLVDEAEGLAVDGSSPVCSAGAGGRLEASAEQQLGLLNSITASDLTALSNSVANVCNGPAEMSCLDSPFFPPLESLHRAALNHDDQLSYGTNGDGVCLNMLDSGFYRSDLGQLSMCSTKDCERPSKRLKMGLSESFINDVDFEGRRAHRITGATMAVSVTDFSGIGSEANAFISSHSRHHQHTALQSD

Target ORF information:

RefSeq Version	NM_001083560.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083560.1

ORF Insert Sequence:

ATGCTGGCTT CCTTGGGGAG TTCGAGCCCT GTTGGCTCTT TGTCTTCAGA GGATCACGAC 
TTTGATCCAA CCGCTGAGAT GTTGATTCAT GAGTATGATG ATGAGAGAAC ATTGGAGGAG 
GAAGAGCTTT TGGAAGGAGA GAGAAATTTC AGTGCAGAAC TGTCCGACTT GGAAAGGGAG 
GGGAACATGC CGATCGAGGA GCTGTTGGCC ATGTATCGTT ACGAGGCGTC AGTCAGTACC 
GGTGCAGGAT CAAGCATTGA CAGTTCCTCT GTTGAACTAG CAGATGATCT GCCTGACATG 
ACCCTGGATA AGGAGGAGAT TGCTAAAGAT CTCCTGTCAG GAGATTATGA GGAGGAGACC 
CAGTCGTCTG CTGATGATCT GACCCCTTCT GTCACTTCCC ATGAGGCCAC GGATTTCTTC 
CCCAGAACTC TAAGATCCAA TGTAACATAT GACGGTGATA AAGAATCTGA GGGGGAGGAG 
GACGGTGTAA ACAGTGAAGA TTCTAGAAAG GAAATAATGG TGGGATCTCA ATATCAAGCA 
GAAGTCCCAG CAGGGCTCAG CCACTATAAT GATGATGAAA AGGTGTACGA GGAGGAAGAT 
CAGCTGCTGT GGTGTCCTGA CGAACTGTCA GAGTATAAAG TGAAAGACTA CCTCAGAGAA 
GCACTGTCAC AGTCTACAGA TGACCGCACG CATTATTCCA CATCTGAACA CATACGAGAC 
AACGAGCAGG CTTTGTTTGA GCTTTTGAAA TGTAATTATG ACACTCGTGA GGCGCTGGTT 
CGATACTGCA GTAATGTAAA GACCTCAAAA GAAGAATCAC CACCATGGTC AGAGGAGGAA 
TGTAGGAACT TCGAACATGC GCTGCAGATT TATGAGAAAA ACTTCCATCT TATTCAGAAA 
CACAAGGTTC AAACCCGGAC TGTAGCAGAA TGTGTGGCTT TTTACTACAT GTGGAAAAAG 
TCGGAACGTT TTGACTACTT TGCTCAACAG AACCGATTTG GCAAGAAGAA ATACAGCTGT 
TATCCTGGTG TGACGGACCT GATGGATCGG CTGGTAGACG AGGCTGAGGG TCTGGCAGTG 
GACGGCTCTT CACCGGTGTG TTCAGCTGGA GCAGGAGGAA GACTAGAGGC ATCGGCAGAG 
CAACAGCTCG GACTGCTCAA CTCCATCACT GCCAGTGACC TCACAGCTCT TAGTAACAGT 
GTAGCGAATG TGTGCAACGG TCCAGCAGAG ATGAGCTGTT TGGACTCTCC GTTCTTCCCC 
CCTCTGGAGA GTTTGCACCG AGCAGCCCTG AACCATGACG ATCAGCTCAG CTACGGAACG 
AATGGAGACG GCGTCTGCCT CAACATGCTG GATTCAGGCT TTTACCGCTC TGACCTGGGC 
CAGCTCAGCA TGTGCAGCAC CAAAGACTGC GAACGACCCT CCAAGAGGCT CAAGATGGGC 
CTTTCAGAGT CCTTCATCAA CGACGTGGAC TTCGAGGGCA GACGAGCACA TCGCATTACA 
GGCGCCACAA TGGCGGTTTC GGTCACAGAC TTCAGTGGCA TCGGGAGTGA AGCCAACGCT 
TTCATCAGCT CTCATTCACG ACACCACCAA CACACTGCAC TTCAGTCCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mier3b ( XM_005166879.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166936.1 Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14016
Clone ID Related Accession (Same CDS sequence)	XM_005166879.4 , XM_005166879.3
Accession Version	XM_005166879.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC567247 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166879.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CTTCCTTGGG GAGTTCGAGC CCTGTTGGCT CTTTGTCTTC AGAGGATCAC 
GACTTTGATC CAACCGCTGA GATGTTGATT CATGAGTATG ATGATGAGAG AACATTGGAA 
GAGGAAGAGC TTTTAGAAGG AGAGAGAAAT TTCAGTGCAG AACTGTCCGA CTTGGAAAGG 
GAGGGGAACA TGCCGATCGA TGAGCTGTTG GCCATGTATC GTTACGAGGC GTCAGTCAGT 
ACTGGTGCAG GATCAAGCAT TGACAGTTCC TCTGTTGAAC TAGCAGATGA TCTGCCTGAC 
ATGACCCTGG ATAAGGAGGA GATTGCTAAA GATCTCCTGT CAGGAGATTA TGAGGAGGAG 
ACCCAGTCGT CTGCTGATGA TCTGACCCCT TCTGTCACTT CCCATGAGGC CACGGATTTC 
TTCCCCAGAA CTCTAAGATC CAATGTAACA TATGACGGTG ATAAAGAATC TGAGGGGGAG 
GAGGACGGTG TAAACAGTGA AGATTCTAGA AAGGAAATAA TGGTGGGATC TCAATATCAA 
GCAGAAGTCC CAGCAGGGCT CAGCCACTAT AATGATGATG AAAAGGTGTA CGAGGAGGAA 
GATCAGCTGC TGTGGTGTCC TGACGAACTG TCAGAGTATA AAGTGAAAGA CTACCTCAGA 
GAAGCACTGT CACAGTCTAC AGATGACCGC ACGCATTATT CCACATCTGA ACACATACGA 
GACAACGAGC AGGCTTTGTT TGAGCTTTTG AAATGTAATT ATGACACTCG TGAGGCGCTG 
GTTCGATACT GCAGTAATGT AAAGACCTCA AAAGAAGAAT CACCACCATG GTCAGAGGAG 
GAATGTAGGA ACTTCGAACA TGCGCTGCAG ATTTATGAGA AAAACTTCCA TCTTATTCAG 
AAACACAAGG TTCAAACACG GACTGTAGCA GAATGTGTGG CTTTTTACTA CATGTGGAAA 
AAGTCAGAAC GTTTTGACTA CTTTGCTCAA CAGAACCGAT TTGGCAAGAA GAAATACAGC 
TGTTACCCTG GTGTGACGGA CCTGATGGAT CGGCTGGTAG ACGAGGCTGA GGGTCTGGCA 
GTGGACGGCT CTTCACCGGT GTGTTCAGCT GGAGCAGGAG GAAGACTAGA GGCATCGGCA 
GAGCAACAGC TCGGACTGCT CAACTCTATC ACTGCCAGTG ACCTCACAGC TCTTAGTAAC 
AGTGTAGCGA ATGTGTGCAA CGGTCCAGCA GAGATGAGCT GTTTGGACTC TCCGTTCTTC 
CCCCCTCTGG AAAGTTTGCA CCGAGCAGCC CTGAACCATG ACGATCAGCT CAGCTACGGA 
ACGAATGGAG ACGGCGTCTG CCTCAACATG CTGGATTCAG GCTTTTACCG CTCTGACCTG 
GGCCAGCTCA GCATGTGCAG CAGCAAAGAC TGCGAACGAC CCTCCAAGAG GCTCAAAATG 
GGCCTTTCAG AGTCCTTCAT CAACGACGTG GACTTCGAGG GCAGACGAGC ACATCGCATC 
ACAGGCGCCA CAATGGCGGT TTCGGTCACA GACTTCAGTG GCATCGGGAG TGAAGCCAAC 
GCTTTCATCA GCTCTCATTC TCGACACCAC CAACACACTG CACTTCAGTC CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166936.1
CDS	51..1667
Translation	MAEASLGSSSPVGSLSSEDHDFDPTAEMLIHEYDDERTLEEEELLEGERNFSAELSDLEREGNMPIDELLAMYRYEASVSTGAGSSIDSSSVELADDLPDMTLDKEEIAKDLLSGDYEEETQSSADDLTPSVTSHEATDFFPRTLRSNVTYDGDKESEGEEDGVNSEDSRKEIMVGSQYQAEVPAGLSHYNDDEKVYEEEDQLLWCPDELSEYKVKDYLREALSQSTDDRTHYSTSEHIRDNEQALFELLKCNYDTREALVRYCSNVKTSKEESPPWSEEECRNFEHALQIYEKNFHLIQKHKVQTRTVAECVAFYYMWKKSERFDYFAQQNRFGKKKYSCYPGVTDLMDRLVDEAEGLAVDGSSPVCSAGAGGRLEASAEQQLGLLNSITASDLTALSNSVANVCNGPAEMSCLDSPFFPPLESLHRAALNHDDQLSYGTNGDGVCLNMLDSGFYRSDLGQLSMCSSKDCERPSKRLKMGLSESFINDVDFEGRRAHRITGATMAVSVTDFSGIGSEANAFISSHSRHHQHTALQSD

Target ORF information:

RefSeq Version	XM_005166879.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166879.4

ORF Insert Sequence:

ATGGCGGAGG CTTCCTTGGG GAGTTCGAGC CCTGTTGGCT CTTTGTCTTC AGAGGATCAC 
GACTTTGATC CAACCGCTGA GATGTTGATT CATGAGTATG ATGATGAGAG AACATTGGAA 
GAGGAAGAGC TTTTAGAAGG AGAGAGAAAT TTCAGTGCAG AACTGTCCGA CTTGGAAAGG 
GAGGGGAACA TGCCGATCGA TGAGCTGTTG GCCATGTATC GTTACGAGGC GTCAGTCAGT 
ACTGGTGCAG GATCAAGCAT TGACAGTTCC TCTGTTGAAC TAGCAGATGA TCTGCCTGAC 
ATGACCCTGG ATAAGGAGGA GATTGCTAAA GATCTCCTGT CAGGAGATTA TGAGGAGGAG 
ACCCAGTCGT CTGCTGATGA TCTGACCCCT TCTGTCACTT CCCATGAGGC CACGGATTTC 
TTCCCCAGAA CTCTAAGATC CAATGTAACA TATGACGGTG ATAAAGAATC TGAGGGGGAG 
GAGGACGGTG TAAACAGTGA AGATTCTAGA AAGGAAATAA TGGTGGGATC TCAATATCAA 
GCAGAAGTCC CAGCAGGGCT CAGCCACTAT AATGATGATG AAAAGGTGTA CGAGGAGGAA 
GATCAGCTGC TGTGGTGTCC TGACGAACTG TCAGAGTATA AAGTGAAAGA CTACCTCAGA 
GAAGCACTGT CACAGTCTAC AGATGACCGC ACGCATTATT CCACATCTGA ACACATACGA 
GACAACGAGC AGGCTTTGTT TGAGCTTTTG AAATGTAATT ATGACACTCG TGAGGCGCTG 
GTTCGATACT GCAGTAATGT AAAGACCTCA AAAGAAGAAT CACCACCATG GTCAGAGGAG 
GAATGTAGGA ACTTCGAACA TGCGCTGCAG ATTTATGAGA AAAACTTCCA TCTTATTCAG 
AAACACAAGG TTCAAACACG GACTGTAGCA GAATGTGTGG CTTTTTACTA CATGTGGAAA 
AAGTCAGAAC GTTTTGACTA CTTTGCTCAA CAGAACCGAT TTGGCAAGAA GAAATACAGC 
TGTTACCCTG GTGTGACGGA CCTGATGGAT CGGCTGGTAG ACGAGGCTGA GGGTCTGGCA 
GTGGACGGCT CTTCACCGGT GTGTTCAGCT GGAGCAGGAG GAAGACTAGA GGCATCGGCA 
GAGCAACAGC TCGGACTGCT CAACTCTATC ACTGCCAGTG ACCTCACAGC TCTTAGTAAC 
AGTGTAGCGA ATGTGTGCAA CGGTCCAGCA GAGATGAGCT GTTTGGACTC TCCGTTCTTC 
CCCCCTCTGG AAAGTTTGCA CCGAGCAGCC CTGAACCATG ACGATCAGCT CAGCTACGGA 
ACGAATGGAG ACGGCGTCTG CCTCAACATG CTGGATTCAG GCTTTTACCG CTCTGACCTG 
GGCCAGCTCA GCATGTGCAG CAGCAAAGAC TGCGAACGAC CCTCCAAGAG GCTCAAAATG 
GGCCTTTCAG AGTCCTTCAT CAACGACGTG GACTTCGAGG GCAGACGAGC ACATCGCATC 
ACAGGCGCCA CAATGGCGGT TTCGGTCACA GACTTCAGTG GCATCGGGAG TGAAGCCAAC 
GCTTTCATCA GCTCTCATTC TCGACACCAC CAACACACTG CACTTCAGTC CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mier3b ( XM_005166879.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166936.1 Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14016
Clone ID Related Accession (Same CDS sequence)	XM_005166879.4 , XM_005166879.3
Accession Version	XM_005166879.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC567247 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166879.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CTTCCTTGGG GAGTTCGAGC CCTGTTGGCT CTTTGTCTTC AGAGGATCAC 
GACTTTGATC CAACCGCTGA GATGTTGATT CATGAGTATG ATGATGAGAG AACATTGGAA 
GAGGAAGAGC TTTTAGAAGG AGAGAGAAAT TTCAGTGCAG AACTGTCCGA CTTGGAAAGG 
GAGGGGAACA TGCCGATCGA TGAGCTGTTG GCCATGTATC GTTACGAGGC GTCAGTCAGT 
ACTGGTGCAG GATCAAGCAT TGACAGTTCC TCTGTTGAAC TAGCAGATGA TCTGCCTGAC 
ATGACCCTGG ATAAGGAGGA GATTGCTAAA GATCTCCTGT CAGGAGATTA TGAGGAGGAG 
ACCCAGTCGT CTGCTGATGA TCTGACCCCT TCTGTCACTT CCCATGAGGC CACGGATTTC 
TTCCCCAGAA CTCTAAGATC CAATGTAACA TATGACGGTG ATAAAGAATC TGAGGGGGAG 
GAGGACGGTG TAAACAGTGA AGATTCTAGA AAGGAAATAA TGGTGGGATC TCAATATCAA 
GCAGAAGTCC CAGCAGGGCT CAGCCACTAT AATGATGATG AAAAGGTGTA CGAGGAGGAA 
GATCAGCTGC TGTGGTGTCC TGACGAACTG TCAGAGTATA AAGTGAAAGA CTACCTCAGA 
GAAGCACTGT CACAGTCTAC AGATGACCGC ACGCATTATT CCACATCTGA ACACATACGA 
GACAACGAGC AGGCTTTGTT TGAGCTTTTG AAATGTAATT ATGACACTCG TGAGGCGCTG 
GTTCGATACT GCAGTAATGT AAAGACCTCA AAAGAAGAAT CACCACCATG GTCAGAGGAG 
GAATGTAGGA ACTTCGAACA TGCGCTGCAG ATTTATGAGA AAAACTTCCA TCTTATTCAG 
AAACACAAGG TTCAAACACG GACTGTAGCA GAATGTGTGG CTTTTTACTA CATGTGGAAA 
AAGTCAGAAC GTTTTGACTA CTTTGCTCAA CAGAACCGAT TTGGCAAGAA GAAATACAGC 
TGTTACCCTG GTGTGACGGA CCTGATGGAT CGGCTGGTAG ACGAGGCTGA GGGTCTGGCA 
GTGGACGGCT CTTCACCGGT GTGTTCAGCT GGAGCAGGAG GAAGACTAGA GGCATCGGCA 
GAGCAACAGC TCGGACTGCT CAACTCTATC ACTGCCAGTG ACCTCACAGC TCTTAGTAAC 
AGTGTAGCGA ATGTGTGCAA CGGTCCAGCA GAGATGAGCT GTTTGGACTC TCCGTTCTTC 
CCCCCTCTGG AAAGTTTGCA CCGAGCAGCC CTGAACCATG ACGATCAGCT CAGCTACGGA 
ACGAATGGAG ACGGCGTCTG CCTCAACATG CTGGATTCAG GCTTTTACCG CTCTGACCTG 
GGCCAGCTCA GCATGTGCAG CAGCAAAGAC TGCGAACGAC CCTCCAAGAG GCTCAAAATG 
GGCCTTTCAG AGTCCTTCAT CAACGACGTG GACTTCGAGG GCAGACGAGC ACATCGCATC 
ACAGGCGCCA CAATGGCGGT TTCGGTCACA GACTTCAGTG GCATCGGGAG TGAAGCCAAC 
GCTTTCATCA GCTCTCATTC TCGACACCAC CAACACACTG CACTTCAGTC CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166936.1
CDS	59..1675
Translation	MAEASLGSSSPVGSLSSEDHDFDPTAEMLIHEYDDERTLEEEELLEGERNFSAELSDLEREGNMPIDELLAMYRYEASVSTGAGSSIDSSSVELADDLPDMTLDKEEIAKDLLSGDYEEETQSSADDLTPSVTSHEATDFFPRTLRSNVTYDGDKESEGEEDGVNSEDSRKEIMVGSQYQAEVPAGLSHYNDDEKVYEEEDQLLWCPDELSEYKVKDYLREALSQSTDDRTHYSTSEHIRDNEQALFELLKCNYDTREALVRYCSNVKTSKEESPPWSEEECRNFEHALQIYEKNFHLIQKHKVQTRTVAECVAFYYMWKKSERFDYFAQQNRFGKKKYSCYPGVTDLMDRLVDEAEGLAVDGSSPVCSAGAGGRLEASAEQQLGLLNSITASDLTALSNSVANVCNGPAEMSCLDSPFFPPLESLHRAALNHDDQLSYGTNGDGVCLNMLDSGFYRSDLGQLSMCSSKDCERPSKRLKMGLSESFINDVDFEGRRAHRITGATMAVSVTDFSGIGSEANAFISSHSRHHQHTALQSD

Target ORF information:

RefSeq Version	XM_005166879.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166879.3

ORF Insert Sequence:

ATGGCGGAGG CTTCCTTGGG GAGTTCGAGC CCTGTTGGCT CTTTGTCTTC AGAGGATCAC 
GACTTTGATC CAACCGCTGA GATGTTGATT CATGAGTATG ATGATGAGAG AACATTGGAA 
GAGGAAGAGC TTTTAGAAGG AGAGAGAAAT TTCAGTGCAG AACTGTCCGA CTTGGAAAGG 
GAGGGGAACA TGCCGATCGA TGAGCTGTTG GCCATGTATC GTTACGAGGC GTCAGTCAGT 
ACTGGTGCAG GATCAAGCAT TGACAGTTCC TCTGTTGAAC TAGCAGATGA TCTGCCTGAC 
ATGACCCTGG ATAAGGAGGA GATTGCTAAA GATCTCCTGT CAGGAGATTA TGAGGAGGAG 
ACCCAGTCGT CTGCTGATGA TCTGACCCCT TCTGTCACTT CCCATGAGGC CACGGATTTC 
TTCCCCAGAA CTCTAAGATC CAATGTAACA TATGACGGTG ATAAAGAATC TGAGGGGGAG 
GAGGACGGTG TAAACAGTGA AGATTCTAGA AAGGAAATAA TGGTGGGATC TCAATATCAA 
GCAGAAGTCC CAGCAGGGCT CAGCCACTAT AATGATGATG AAAAGGTGTA CGAGGAGGAA 
GATCAGCTGC TGTGGTGTCC TGACGAACTG TCAGAGTATA AAGTGAAAGA CTACCTCAGA 
GAAGCACTGT CACAGTCTAC AGATGACCGC ACGCATTATT CCACATCTGA ACACATACGA 
GACAACGAGC AGGCTTTGTT TGAGCTTTTG AAATGTAATT ATGACACTCG TGAGGCGCTG 
GTTCGATACT GCAGTAATGT AAAGACCTCA AAAGAAGAAT CACCACCATG GTCAGAGGAG 
GAATGTAGGA ACTTCGAACA TGCGCTGCAG ATTTATGAGA AAAACTTCCA TCTTATTCAG 
AAACACAAGG TTCAAACACG GACTGTAGCA GAATGTGTGG CTTTTTACTA CATGTGGAAA 
AAGTCAGAAC GTTTTGACTA CTTTGCTCAA CAGAACCGAT TTGGCAAGAA GAAATACAGC 
TGTTACCCTG GTGTGACGGA CCTGATGGAT CGGCTGGTAG ACGAGGCTGA GGGTCTGGCA 
GTGGACGGCT CTTCACCGGT GTGTTCAGCT GGAGCAGGAG GAAGACTAGA GGCATCGGCA 
GAGCAACAGC TCGGACTGCT CAACTCTATC ACTGCCAGTG ACCTCACAGC TCTTAGTAAC 
AGTGTAGCGA ATGTGTGCAA CGGTCCAGCA GAGATGAGCT GTTTGGACTC TCCGTTCTTC 
CCCCCTCTGG AAAGTTTGCA CCGAGCAGCC CTGAACCATG ACGATCAGCT CAGCTACGGA 
ACGAATGGAG ACGGCGTCTG CCTCAACATG CTGGATTCAG GCTTTTACCG CTCTGACCTG 
GGCCAGCTCA GCATGTGCAG CAGCAAAGAC TGCGAACGAC CCTCCAAGAG GCTCAAAATG 
GGCCTTTCAG AGTCCTTCAT CAACGACGTG GACTTCGAGG GCAGACGAGC ACATCGCATC 
ACAGGCGCCA CAATGGCGGT TTCGGTCACA GACTTCAGTG GCATCGGGAG TGAAGCCAAC 
GCTTTCATCA GCTCTCATTC TCGACACCAC CAACACACTG CACTTCAGTC CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mier3b ( XM_005166880.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166937.1 Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), transcript variant X2, mRNA.

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CloneID	ODa14017
Clone ID Related Accession (Same CDS sequence)	XM_005166880.3 , XM_005166880.4
Accession Version	XM_005166880.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC567247 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CTTCCTTGGG GAGTTCGAGC CCTGTTGGCT CTTTGTCTTC AGAGGATCAC 
GACTTTGATC CAACCGCTGA GATGTTGATT CATGAGTATG ATGATGAGAG AACATTGGAA 
GAGGAAGAGC TTTTAGAAGG AGAGAGAAAT TTCAGTGCAG AACTGTCCGA CTTGGAAAGG 
GAGGGGAACA TGCCGATCGA TGAGCTGTTG GCCATGTATC GTTACGAGGC GTCAGTCAGT 
ACTGGTGCAG GATCAAGCAT TGACAGTTCC TCTGTTGAAC TAGCAGATGA TCTGCCTGAC 
ATGACCCTGG ATAAGGAGGA GATTGCTAAA GATCTCCTGT CAGGAGATTA TGAGGAGGAG 
ACCCAGTCGT CTGCTGATGA TCTGACCCCT TCTGTCACTT CCCATGAGGC CACGGATTTC 
TTCCCCAGAA CTCTAAGATC CAATGTAACA TATGACGGTG ATAAAGAATC TGAGGGGGAG 
GAGGACGGTG TAAACAGTGA AGATTCTAGA AAGGAAATAA TGGTGGGATC TCAATATCAA 
GCAGAAGTCC CAGCAGGGCT CAGCCACTAT AATGATGATG AAAAGGTGTA CGAGGAGGAA 
GATCAGCTGC TGTGGTGTCC TGACGAACTG TCAGAGTATA AAGTGAAAGA CTACCTCAGA 
GAAGCACTGT CACAGTCTAC AGATGACCGC ACGCATTATT CCACATCTGA ACACATACGA 
GACAACGAGC AGGCTTTGTT TGAGCTTTTG AAATGTAATT ATGACACTCG TGAGGCGCTG 
GTTCGATACT GCAGTAATGT AAAGACCTCA AAAGAATCAC CACCATGGTC AGAGGAGGAA 
TGTAGGAACT TCGAACATGC GCTGCAGATT TATGAGAAAA ACTTCCATCT TATTCAGAAA 
CACAAGGTTC AAACACGGAC TGTAGCAGAA TGTGTGGCTT TTTACTACAT GTGGAAAAAG 
TCAGAACGTT TTGACTACTT TGCTCAACAG AACCGATTTG GCAAGAAGAA ATACAGCTGT 
TACCCTGGTG TGACGGACCT GATGGATCGG CTGGTAGACG AGGCTGAGGG TCTGGCAGTG 
GACGGCTCTT CACCGGTGTG TTCAGCTGGA GCAGGAGGAA GACTAGAGGC ATCGGCAGAG 
CAACAGCTCG GACTGCTCAA CTCTATCACT GCCAGTGACC TCACAGCTCT TAGTAACAGT 
GTAGCGAATG TGTGCAACGG TCCAGCAGAG ATGAGCTGTT TGGACTCTCC GTTCTTCCCC 
CCTCTGGAAA GTTTGCACCG AGCAGCCCTG AACCATGACG ATCAGCTCAG CTACGGAACG 
AATGGAGACG GCGTCTGCCT CAACATGCTG GATTCAGGCT TTTACCGCTC TGACCTGGGC 
CAGCTCAGCA TGTGCAGCAG CAAAGACTGC GAACGACCCT CCAAGAGGCT CAAAATGGGC 
CTTTCAGAGT CCTTCATCAA CGACGTGGAC TTCGAGGGCA GACGAGCACA TCGCATCACA 
GGCGCCACAA TGGCGGTTTC GGTCACAGAC TTCAGTGGCA TCGGGAGTGA AGCCAACGCT 
TTCATCAGCT CTCATTCTCG ACACCACCAA CACACTGCAC TTCAGTCCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166937.1
CDS	62..1675
Translation	MAEASLGSSSPVGSLSSEDHDFDPTAEMLIHEYDDERTLEEEELLEGERNFSAELSDLEREGNMPIDELLAMYRYEASVSTGAGSSIDSSSVELADDLPDMTLDKEEIAKDLLSGDYEEETQSSADDLTPSVTSHEATDFFPRTLRSNVTYDGDKESEGEEDGVNSEDSRKEIMVGSQYQAEVPAGLSHYNDDEKVYEEEDQLLWCPDELSEYKVKDYLREALSQSTDDRTHYSTSEHIRDNEQALFELLKCNYDTREALVRYCSNVKTSKESPPWSEEECRNFEHALQIYEKNFHLIQKHKVQTRTVAECVAFYYMWKKSERFDYFAQQNRFGKKKYSCYPGVTDLMDRLVDEAEGLAVDGSSPVCSAGAGGRLEASAEQQLGLLNSITASDLTALSNSVANVCNGPAEMSCLDSPFFPPLESLHRAALNHDDQLSYGTNGDGVCLNMLDSGFYRSDLGQLSMCSSKDCERPSKRLKMGLSESFINDVDFEGRRAHRITGATMAVSVTDFSGIGSEANAFISSHSRHHQHTALQSD

Target ORF information:

RefSeq Version	XM_005166880.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166880.3

ORF Insert Sequence:

ATGGCGGAGG CTTCCTTGGG GAGTTCGAGC CCTGTTGGCT CTTTGTCTTC AGAGGATCAC 
GACTTTGATC CAACCGCTGA GATGTTGATT CATGAGTATG ATGATGAGAG AACATTGGAA 
GAGGAAGAGC TTTTAGAAGG AGAGAGAAAT TTCAGTGCAG AACTGTCCGA CTTGGAAAGG 
GAGGGGAACA TGCCGATCGA TGAGCTGTTG GCCATGTATC GTTACGAGGC GTCAGTCAGT 
ACTGGTGCAG GATCAAGCAT TGACAGTTCC TCTGTTGAAC TAGCAGATGA TCTGCCTGAC 
ATGACCCTGG ATAAGGAGGA GATTGCTAAA GATCTCCTGT CAGGAGATTA TGAGGAGGAG 
ACCCAGTCGT CTGCTGATGA TCTGACCCCT TCTGTCACTT CCCATGAGGC CACGGATTTC 
TTCCCCAGAA CTCTAAGATC CAATGTAACA TATGACGGTG ATAAAGAATC TGAGGGGGAG 
GAGGACGGTG TAAACAGTGA AGATTCTAGA AAGGAAATAA TGGTGGGATC TCAATATCAA 
GCAGAAGTCC CAGCAGGGCT CAGCCACTAT AATGATGATG AAAAGGTGTA CGAGGAGGAA 
GATCAGCTGC TGTGGTGTCC TGACGAACTG TCAGAGTATA AAGTGAAAGA CTACCTCAGA 
GAAGCACTGT CACAGTCTAC AGATGACCGC ACGCATTATT CCACATCTGA ACACATACGA 
GACAACGAGC AGGCTTTGTT TGAGCTTTTG AAATGTAATT ATGACACTCG TGAGGCGCTG 
GTTCGATACT GCAGTAATGT AAAGACCTCA AAAGAATCAC CACCATGGTC AGAGGAGGAA 
TGTAGGAACT TCGAACATGC GCTGCAGATT TATGAGAAAA ACTTCCATCT TATTCAGAAA 
CACAAGGTTC AAACACGGAC TGTAGCAGAA TGTGTGGCTT TTTACTACAT GTGGAAAAAG 
TCAGAACGTT TTGACTACTT TGCTCAACAG AACCGATTTG GCAAGAAGAA ATACAGCTGT 
TACCCTGGTG TGACGGACCT GATGGATCGG CTGGTAGACG AGGCTGAGGG TCTGGCAGTG 
GACGGCTCTT CACCGGTGTG TTCAGCTGGA GCAGGAGGAA GACTAGAGGC ATCGGCAGAG 
CAACAGCTCG GACTGCTCAA CTCTATCACT GCCAGTGACC TCACAGCTCT TAGTAACAGT 
GTAGCGAATG TGTGCAACGG TCCAGCAGAG ATGAGCTGTT TGGACTCTCC GTTCTTCCCC 
CCTCTGGAAA GTTTGCACCG AGCAGCCCTG AACCATGACG ATCAGCTCAG CTACGGAACG 
AATGGAGACG GCGTCTGCCT CAACATGCTG GATTCAGGCT TTTACCGCTC TGACCTGGGC 
CAGCTCAGCA TGTGCAGCAG CAAAGACTGC GAACGACCCT CCAAGAGGCT CAAAATGGGC 
CTTTCAGAGT CCTTCATCAA CGACGTGGAC TTCGAGGGCA GACGAGCACA TCGCATCACA 
GGCGCCACAA TGGCGGTTTC GGTCACAGAC TTCAGTGGCA TCGGGAGTGA AGCCAACGCT 
TTCATCAGCT CTCATTCTCG ACACCACCAA CACACTGCAC TTCAGTCCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mier3b ( XM_005166880.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166937.1 Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), transcript variant X2, mRNA.

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CloneID	ODa14017
Clone ID Related Accession (Same CDS sequence)	XM_005166880.3 , XM_005166880.4
Accession Version	XM_005166880.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC567247 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166880.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CTTCCTTGGG GAGTTCGAGC CCTGTTGGCT CTTTGTCTTC AGAGGATCAC 
GACTTTGATC CAACCGCTGA GATGTTGATT CATGAGTATG ATGATGAGAG AACATTGGAA 
GAGGAAGAGC TTTTAGAAGG AGAGAGAAAT TTCAGTGCAG AACTGTCCGA CTTGGAAAGG 
GAGGGGAACA TGCCGATCGA TGAGCTGTTG GCCATGTATC GTTACGAGGC GTCAGTCAGT 
ACTGGTGCAG GATCAAGCAT TGACAGTTCC TCTGTTGAAC TAGCAGATGA TCTGCCTGAC 
ATGACCCTGG ATAAGGAGGA GATTGCTAAA GATCTCCTGT CAGGAGATTA TGAGGAGGAG 
ACCCAGTCGT CTGCTGATGA TCTGACCCCT TCTGTCACTT CCCATGAGGC CACGGATTTC 
TTCCCCAGAA CTCTAAGATC CAATGTAACA TATGACGGTG ATAAAGAATC TGAGGGGGAG 
GAGGACGGTG TAAACAGTGA AGATTCTAGA AAGGAAATAA TGGTGGGATC TCAATATCAA 
GCAGAAGTCC CAGCAGGGCT CAGCCACTAT AATGATGATG AAAAGGTGTA CGAGGAGGAA 
GATCAGCTGC TGTGGTGTCC TGACGAACTG TCAGAGTATA AAGTGAAAGA CTACCTCAGA 
GAAGCACTGT CACAGTCTAC AGATGACCGC ACGCATTATT CCACATCTGA ACACATACGA 
GACAACGAGC AGGCTTTGTT TGAGCTTTTG AAATGTAATT ATGACACTCG TGAGGCGCTG 
GTTCGATACT GCAGTAATGT AAAGACCTCA AAAGAATCAC CACCATGGTC AGAGGAGGAA 
TGTAGGAACT TCGAACATGC GCTGCAGATT TATGAGAAAA ACTTCCATCT TATTCAGAAA 
CACAAGGTTC AAACACGGAC TGTAGCAGAA TGTGTGGCTT TTTACTACAT GTGGAAAAAG 
TCAGAACGTT TTGACTACTT TGCTCAACAG AACCGATTTG GCAAGAAGAA ATACAGCTGT 
TACCCTGGTG TGACGGACCT GATGGATCGG CTGGTAGACG AGGCTGAGGG TCTGGCAGTG 
GACGGCTCTT CACCGGTGTG TTCAGCTGGA GCAGGAGGAA GACTAGAGGC ATCGGCAGAG 
CAACAGCTCG GACTGCTCAA CTCTATCACT GCCAGTGACC TCACAGCTCT TAGTAACAGT 
GTAGCGAATG TGTGCAACGG TCCAGCAGAG ATGAGCTGTT TGGACTCTCC GTTCTTCCCC 
CCTCTGGAAA GTTTGCACCG AGCAGCCCTG AACCATGACG ATCAGCTCAG CTACGGAACG 
AATGGAGACG GCGTCTGCCT CAACATGCTG GATTCAGGCT TTTACCGCTC TGACCTGGGC 
CAGCTCAGCA TGTGCAGCAG CAAAGACTGC GAACGACCCT CCAAGAGGCT CAAAATGGGC 
CTTTCAGAGT CCTTCATCAA CGACGTGGAC TTCGAGGGCA GACGAGCACA TCGCATCACA 
GGCGCCACAA TGGCGGTTTC GGTCACAGAC TTCAGTGGCA TCGGGAGTGA AGCCAACGCT 
TTCATCAGCT CTCATTCTCG ACACCACCAA CACACTGCAC TTCAGTCCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166937.1
CDS	51..1664
Translation	MAEASLGSSSPVGSLSSEDHDFDPTAEMLIHEYDDERTLEEEELLEGERNFSAELSDLEREGNMPIDELLAMYRYEASVSTGAGSSIDSSSVELADDLPDMTLDKEEIAKDLLSGDYEEETQSSADDLTPSVTSHEATDFFPRTLRSNVTYDGDKESEGEEDGVNSEDSRKEIMVGSQYQAEVPAGLSHYNDDEKVYEEEDQLLWCPDELSEYKVKDYLREALSQSTDDRTHYSTSEHIRDNEQALFELLKCNYDTREALVRYCSNVKTSKESPPWSEEECRNFEHALQIYEKNFHLIQKHKVQTRTVAECVAFYYMWKKSERFDYFAQQNRFGKKKYSCYPGVTDLMDRLVDEAEGLAVDGSSPVCSAGAGGRLEASAEQQLGLLNSITASDLTALSNSVANVCNGPAEMSCLDSPFFPPLESLHRAALNHDDQLSYGTNGDGVCLNMLDSGFYRSDLGQLSMCSSKDCERPSKRLKMGLSESFINDVDFEGRRAHRITGATMAVSVTDFSGIGSEANAFISSHSRHHQHTALQSD

Target ORF information:

RefSeq Version	XM_005166880.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mesoderm induction early response 1, family member 3 b (mier3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166880.4

ORF Insert Sequence:

ATGGCGGAGG CTTCCTTGGG GAGTTCGAGC CCTGTTGGCT CTTTGTCTTC AGAGGATCAC 
GACTTTGATC CAACCGCTGA GATGTTGATT CATGAGTATG ATGATGAGAG AACATTGGAA 
GAGGAAGAGC TTTTAGAAGG AGAGAGAAAT TTCAGTGCAG AACTGTCCGA CTTGGAAAGG 
GAGGGGAACA TGCCGATCGA TGAGCTGTTG GCCATGTATC GTTACGAGGC GTCAGTCAGT 
ACTGGTGCAG GATCAAGCAT TGACAGTTCC TCTGTTGAAC TAGCAGATGA TCTGCCTGAC 
ATGACCCTGG ATAAGGAGGA GATTGCTAAA GATCTCCTGT CAGGAGATTA TGAGGAGGAG 
ACCCAGTCGT CTGCTGATGA TCTGACCCCT TCTGTCACTT CCCATGAGGC CACGGATTTC 
TTCCCCAGAA CTCTAAGATC CAATGTAACA TATGACGGTG ATAAAGAATC TGAGGGGGAG 
GAGGACGGTG TAAACAGTGA AGATTCTAGA AAGGAAATAA TGGTGGGATC TCAATATCAA 
GCAGAAGTCC CAGCAGGGCT CAGCCACTAT AATGATGATG AAAAGGTGTA CGAGGAGGAA 
GATCAGCTGC TGTGGTGTCC TGACGAACTG TCAGAGTATA AAGTGAAAGA CTACCTCAGA 
GAAGCACTGT CACAGTCTAC AGATGACCGC ACGCATTATT CCACATCTGA ACACATACGA 
GACAACGAGC AGGCTTTGTT TGAGCTTTTG AAATGTAATT ATGACACTCG TGAGGCGCTG 
GTTCGATACT GCAGTAATGT AAAGACCTCA AAAGAATCAC CACCATGGTC AGAGGAGGAA 
TGTAGGAACT TCGAACATGC GCTGCAGATT TATGAGAAAA ACTTCCATCT TATTCAGAAA 
CACAAGGTTC AAACACGGAC TGTAGCAGAA TGTGTGGCTT TTTACTACAT GTGGAAAAAG 
TCAGAACGTT TTGACTACTT TGCTCAACAG AACCGATTTG GCAAGAAGAA ATACAGCTGT 
TACCCTGGTG TGACGGACCT GATGGATCGG CTGGTAGACG AGGCTGAGGG TCTGGCAGTG 
GACGGCTCTT CACCGGTGTG TTCAGCTGGA GCAGGAGGAA GACTAGAGGC ATCGGCAGAG 
CAACAGCTCG GACTGCTCAA CTCTATCACT GCCAGTGACC TCACAGCTCT TAGTAACAGT 
GTAGCGAATG TGTGCAACGG TCCAGCAGAG ATGAGCTGTT TGGACTCTCC GTTCTTCCCC 
CCTCTGGAAA GTTTGCACCG AGCAGCCCTG AACCATGACG ATCAGCTCAG CTACGGAACG 
AATGGAGACG GCGTCTGCCT CAACATGCTG GATTCAGGCT TTTACCGCTC TGACCTGGGC 
CAGCTCAGCA TGTGCAGCAG CAAAGACTGC GAACGACCCT CCAAGAGGCT CAAAATGGGC 
CTTTCAGAGT CCTTCATCAA CGACGTGGAC TTCGAGGGCA GACGAGCACA TCGCATCACA 
GGCGCCACAA TGGCGGTTTC GGTCACAGAC TTCAGTGGCA TCGGGAGTGA AGCCAACGCT 
TTCATCAGCT CTCATTCTCG ACACCACCAA CACACTGCAC TTCAGTCCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development.
Developmental biology404(2)49-60(2015 Aug)
Yang H,Fang L,Zhan R,Hegarty JM,Ren J,Hsiai TK,Gleeson JG,Miller YI,Trejo J,Chi NC

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

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mier3b cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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