RIF1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	RIF1
Entrez Gene ID	567001
Full Name	replication timing regulatory factor 1
Synonyms	fc38c03,si:dkey-89p3.2,wu:fc38c03
General protein information	Preferred Names replication timing regulatory factor 1 Names telomere-associated protein RIF1 RAP1 interacting factor homolog
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005167575.3	XP_005167632.1	telomere-associated protein RIF1 isoform X1
XM_005167575.4	XP_005167632.1	telomere-associated protein RIF1 isoform X1
NM_001080806.1	NP_001074275.1	telomere-associated protein RIF1
XM_009304633.2	XP_009302908.1	telomere-associated protein RIF1 isoform X2
XM_009304633.3	XP_009302908.1	telomere-associated protein RIF1 isoform X2
XM_009304632.2	XP_009302907.1	telomere-associated protein RIF1 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RIF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RIF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03762	NM_001080806.1 Latest version!	Danio rerio replication timing regulatory factor 1 (rif1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa15091	XM_005167575.4 Latest version!	Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa15091	XM_009304632.2 Latest version!	Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa45048	XM_009304633.3 Latest version!	Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
ODa15091	XM_005167575.3	Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa45048	XM_009304633.2	Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RIF1 ( NM_001080806.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001074275.1 Danio rerio replication timing regulatory factor 1 (rif1), mRNA.

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CloneID	ODa03762
Clone ID Related Accession (Same CDS sequence)	NM_001080806.1
Accession Version	NM_001080806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529942400000
Organism	Danio rerio(zebrafish)
Product	telomere-associated protein RIF1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL974314.21. On Mar 11, 2007 this sequence version replaced XM_690286.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCAG TGCCTTTATC CAGTACCAGC CTCCTTCCTC TTCTGGAGTG TTTGGAAGAT 
GAATCAGCTG GGCTTTCAGC GCAGACGGAT GCCTACATCA CCATTGCAAA CCGACTTAAT 
GGAGAAGAAG GACGCCAGTT TCTGCCTGTG GTTGTTAAGC ATTTTGCACG TCTTAGTAAA 
GTTCACATCT CCAGTGAGAA TGAGGAGCTG TGTCAGGCTG CACTGCAGGC TTTGGGATTC 
TGTGTCTTCC ACTCACACAT AGTTTCAGTC ATACCAGCAA ATATTTCAGA GAATATATTG 
TCTACGCTCT GCAATGTTGT GGTAAAGTCC AAAGAGAAGT TTACGTGCAC TCGGGCATTG 
TGGGTCATTT CAAAACAGAA TTTCCCTCCA GAGGTGGCAT CCAAAATGAT TCTCAAGAGC 
CTAGAAGCGA TGCAGACCAG AGAGGTGCAG TCCATCCTCA TTGACCACGA GTCACTCAAT 
GTCATCATAA GGTTACTGGA GCAGGCCCCT GTTCCGATGG CTGTTGGAGC TGTTTGCTGG 
GCTAAACTTG TTATTCCACT GGTGGTTCAT TCGGCCAATA AAGTGCGTCT GCGAGCGGCA 
GCTGCCCTGG AGCTCGGCAT GCCTCTTCTT CTGGAGAAAC AGCAGGAAGT GGCAGCCATT 
GTTGAGCCAA TGATGTCTTC TGTGCTCATC CCTGAAATGC AGAAGCTCTT TGCGTCTAAG 
AACGAGACAA ACATGTTGAA ACTCTGGCCT TTGTTTGTAA GACTTCTGGG CAAGTTACTC 
CACAAAGGCG GTGCTTTCAT TAACTCCTTG CTGTATCTAG AGGAGCTGGG TTTCCGCAAC 
TCTTCTCCTA ATATTAAGAA GATTGCTTTC ATAGCTTGGA AGAGCCTCAT TGACAATTTT 
GCCCTTAATC CAGATATCCT GTGCAGTAGC AAGCGTCTGA AGCTCCTCAT GCAGCCGCTC 
AGCTCCATCC AAGTCAGGAC TGAAGCTCTT CTGCTTACCA AGCTGGAGGT CTGGTGGTAT 
CTTGCTGTCA AGCTCGGACC CAACCTGTCT GCTAACTTTG AACAGAGCCC GACTACACCT 
GCTCGAACCT CTAACCCAAG CAATGGCACA CCTAACACCC CTAAGTCAGG CATTCCCTCC 
TGCAGCACGC CGAGCACGAC TCCTCGCATG AATCTGAACA GCAGCATGCA GGCGCCTCAG 
TCCTATCGTT CCATTCAGCT CCTGGGACTA GAGATGCTCC TGCACTGGCT TGTGGGTCCA 
GAGGTCACAG TCACAGCTGC TCGAGAAAAC CTTCAGCTCA GCCTGGAGCC CCTGACGCAC 
CCGCTTTTTA CCAGCCCTTC CACCTTCAGC AGACATGCTT CTACCCTCAT ATCTGCCAGC 
AAAGAAGGCA TCATTACTGT AGGGAAGCAT GCTCCAGGAA ATAAAAAGGA GCGACAGGGT 
TCAGAGGTCC TCACACTTTT CCTGCAGGCA TTGCAGTCCA TTTTGTCATC AGACGCTCTG 
CCTCCTGAAC GTGCACTGCT TCTGTTGGAA GCCACTGTAA AGGGAATACC TCAGAAGGTG 
CTTGGTTCTG CAGCTTATCA AGTGGCAAAC ATGGACGTGT TAAACGGTAC TCCTGCACTT 
TTCCTAATAC TGCTGTTCTA TAAAAGTAAC CAGCTCTCAT CATTTCTTGA AGACGAAAGA 
TTTTTTTCAA GCTTGGAGAC CCTTGTAAGC TGTGGGTTTT GTGGACCCAC ATCGCCCTTG 
GCATTTGGAG AGGCGGTTTT AGGAGCAGTT AATGGGAGTG TGGAGACTGT TAAAAACAAG 
AAGCAGCTCT GGAGAATGTG GAGTATGGTT GTAAGTCCTC TGACGGACAC AATTACTCAG 
ACCAATGAAG TCAACCAAGG CGATGCTCTG GAGCATAACT TCAGTGCCAT TTACAGTGCG 
TTGATGTTCC CCGTCATCCA CCTGCTGCCT TATTCAGCCT TACCACAGAC CACTCAGAAG 
GCCATGATCA GCACATGGTC CAGGCTTTAC AAGGCTTTTG CCCGTTGCTC AGCTTTGGTT 
GCCACAGCAG AGGAGAATAC ATGCTGTGAA GAGTTGTGTG TCAAGATCTC TGCCTCTCTT 
GACCGTGAAG CTCTAAAAAG TCTGTCTATG CTAGATGCAA TAGCACACAT CTTGCTCATC 
ATCGTTGAGA GTATGGACTT TTCTCCATAT ACCCCTCAGT TCCAGCAGAA GATGAAATCT 
CCTCGTACAC CCATGAGTTG GGTACGCAAG AGAAGCAGAG CCCTCGGGAA TCTCTCAACC 
TTCCACACTC TTCTGATACA GACTCTAGAG GCTTTTCTCA CTGCAGACCA TTCAGAAGAA 
TCTGCAGAGC CTTTAAATGG AATTGGCTCT GCCTTGGTGT CAATCTTGAC CTCTCTTTTC 
ACTAATATTA CCCTTCCCTC ATTCTTACAA GAGGTGCTCT CCAGCCTCAC CAAACCACTT 
GCACGTCTTT ATGAGCAGTT CTCCTGTTGT GATGACCCAT CAAAGCTCAG TCCAGAACTA 
GGGCACAAGC TGGAGAAACT GGCCGCAGAC TTGCTAGGCT GCCTTCAGAC ACATTTGACT 
CTCCATGATG ATGAATTCCT CACTGTGTTG TCGCCCTTGC TTTGTGTACT TTTTTTGCAC 
AAGAGCAAAC AGGTTTGCTC TGTGGTCACT CAATTCTGGA ATGCCACTTT TGGAAATGCG 
CTCACCTTGA GTTACCCAGA ACCTCTGAAG TGTGTTTTAA ATCAGGCTAA GCAGAAGACT 
CCACTGATTT TACCTGGTTT TGAAGTTGTT GATGTACCAG ATGATTACAG TGGAACATAT 
ATGAGTGAGA GTTCTCAGCT GGAGCCTCAG ATTAGCGGGG TAAAAGTGAC CTCTGTGGGA 
AAGAGGGAAT CTCTTTTGGA AAGAGCAGAA GAGCTAACAG GAAAAAGTTC TGTAACCACA 
TCAAAACCAG TGTCTGTAAA GCTGGACTTT GGCTCCCCCA AGCCCTTAAG ACGAGAAGCT 
CTTGAGGAGG AAGCATCTGA AGATTTTGTT TTTATTCCAC CTGAGATGAA AGAGAGAGTC 
CTGACTGAGC ATCAGAAGGA AGTGAAAAGG ACTAAACGGG TTGACATTCC TGCCCTGTAC 
AATAATCTTG ATGCCTCCAT GGACACCACA GTCTTTTCAC AGTATACTCA GAGTCAGGAG 
GAGTCAATGG ACACATTGCC TAAAGGTGAG AAGGATCATC CTGTGGAAGC GGCTAGTGTT 
CAACTGAGGG AGGTTGCCTC AAATGAAGTA CCAACCACAG ATGTTAAAGT CCAAAAAGTC 
ACCAGTTCTG ATATAGAAAC TCCAGATAAA GCTACGCCGA ATAAAGATCA AACTTTGGAG 
ATGGATCAAT CACCATCTAC TGGTGTCTCA GTTGATGTGA GTGGGCTTGA GAAAAGCGCA 
TTGGAGGGGA CAAGTCCTAA TATTTCAAGT TCATCTGATG TGGTATCTGG CACACCTCCT 
AAACCTAACA GCAGGCGTCA GTCATTTATA ACCTTAGAAA AATATGCTGA AGGGAAATCG 
ACAAGTCCTG CAAGTCCTGC ATTTACAGGT ACGCTCACCC GGAGATCAAG AAGACTGGAG 
GCTTCACAGG CATCAACATC AAATCAAAAT GATTCTCAAC CTCAGATTAA AAAAGTGGAT 
TCTCAGGAAT CTCAAAAGAT GAGCAGCATT GACATGTCCA TTCAAAATGA GCATGCTAGC 
ATGGAAGTAA AAGATGAGAA ATCAGTGGGA GCTAATCCAT GTGAAGGTAC AGATGAGGAA 
GATGTTGTTC CAGATACCCA AACCCAAGTA TCTGGTGACA CATTGGGTAT TAAAAGCAGT 
CCTGTGTCTA AAGCCCATGC GGAGGCACAG GGGACTCCTG AAAAAAATTC ACAGAGTCCT 
AATGATTCTC TAGCTAGTCA AGAACCAAGA AGGTCTGGCA GAAGGCGAAG TAAACCTGTT 
CGTCCAGGGC AAGATGCAGA GGAAATAAAA AGTAAATCTC AAGAACAAGC CAGTCAACCT 
GCAGACTCTG CGATAATAAA CTCACAAAAG CCCCTTATTG CTTCACCTAG TAGTACTTTG 
ACTGGAAGAA GGAAAAAAAA GGTTGAGACC AATAAAATGG AAGACCAGCT GAACAATGAA 
GAAATGAAAG GAGATCTCAG CCAAAGCGAT TCACAGATTT CCGCTGCTGA CCCTCCACAG 
ACCGTAGGTG TGCATGCCAA TAAAGACTTA AGCCAGACTG AGTTATCTCC GAAGAGTTCT 
CCTGTAACCC AGTCAAATAG CCAAAGCAAA GAGAGACTGC CAGCTCTCTG CGAGTCTGAG 
AGTATGTTGT TAGGTAGACA GGAGACAAAA ACACAACTTG AAGAATCCAC AGAAAAGGAG 
ACAAATAATG ACTCTCAAAA CGCTTCATCT GCAGCGAGAC AGAAGCATAT ACAGTCTACC 
ACGTCTGACT CCGAGTCGGA TAGCCAGCAA ATTGTCCGTA AACGAAGATC GAGGAGATCT 
GAAACTAAGT TACTGGAGAT ATCTGGTGTG CCGATCAAAG AAGACTTGAA TCAGACAGAC 
TCCCAAACTC CAACAGGAGT GAAGGGAAGA GCTATGAGGA GGAGAACCGT AGAAGGTGCA 
GAGTCTAGTA AAGATTCCAC ATCAGCGGTA AATACAGAGG AGTTAAGTGC AGGCAAGGAG 
TCATCACAAG AGCTTGGTAG ATACAGAACT CGACGTTCTA GGGGTTTGTT GTCTCCTGAG 
AGTATGGAGT CTGAAAACTC TGATAGCCGA GAAGATGGGG CAAGGCCTAA AAAGAGGCTG 
CGAAAACCAC CATCAACAGA GGTGTTACCT GTTGCTTCAG AACCCAAAGT TATTGAGATG 
GTACCAGAAA AGGACGATGT TCAGTCAGAG GTTTTAATGG AGACGTCAAA GGATGAGGCA 
GATCCTGAAA ATATCTCGAA TACTTCAGTC AGTGAGACCA AAGAACTTGA GCAAAATAAT 
AAAGAGGAAG TCCCTGCCCC TTGTGGTGCA CCACATGAAC AGATTATCAT GGAAAAAGCA 
GCATCAAACA ACAAAATTGA CACTGACTTA ACTGATACAG ACGAAAAAGA AACCGTCAAG 
GCAGAGGGTG AAGAAGCTAA TGAATCTTCG CAAACTGGAT CAGAAGATTT TAAAAAGACA 
GATGTTTTGC ATAAATGCCC ACATACAAAG GGACGGGGCC GTGCACGTAG ACGATCTAAA 
AACTGCAACT GCTTTTCCAA CAACCGTGAT GTGTTTTCAC AGGACAGTGA CTTCCAAGAA 
TCACAAGATC TAAAAAATGA ACAGGTTCCA CTTGCTGCCA ACATCTTGAA CTCTCAACCT 
GATCAGTCAA GCCCAACAAC TGCATCAGAA GAAAACAAAA GCTCAGCAAT TGAAGAAATC 
CCTGTTGCTG ATGTTCCATC ACTTTCAGCC TTACCTGCTG ATCTCCCATC TGAGGTTTCG 
CCTTCTGAAA GTCCCATTAC AAAACTTGGG GTAATTGTTT CTCAGCTACC AGAAGTGGAG 
AAAGAAGCAC AGCCTGGATC ATCCAAACCA ATGAAGGATT CGAGCAAGGA GCTTGTAGAA 
GAAGTGAATC GATTAAGTTC TGGATCCACA GGTGGTCAAA AGCACAAAGG AGAAAATAGA 
TTAGAAAACA CTTCAGTAAG CCAAGAACCT GTCCAGAGTT CAACACCTCA AGACACCTCT 
CCAAGTCAAG GTCGTCCAGT GGACGATGCA CCAATATGCC AGGAGCAGCT AGAGTCTTCT 
CATGTCCAGC AGGAAGATGC AGTTAACCCA GCAGTGATGG AAAGAGAAGA CCTTGCTGTG 
TCAGAGCAGA GACAAGAGAA GGCTGTGTTG TCTGAAGAGT CGGATGGAAA AGGTTTGCTT 
GACAAGGAAA TTACTCTTGT TGAAAAGGAA GAAGAGATGC AGGTTAATAA CCAGGATCTT 
TCTGTTGATG TCCAAGAAGA GTTACCTGTA GTGCCCCAGA GTAGCACATC TGAAGCCTGC 
TTGGATTCTC CACCCAAGCC AAAACTGTTA AGCGCACTCA GTGGTGAACT GGAGCCTGGC 
CAGAGTCCAA CTAGAAGTCG GTCTCGAGTC TGGTCACCAT CTGCTTCTCC GTCTACCAGT 
ATTTTGAAGA AGGGTCAGAA GAGAGCATGT GAAGAGGAGA CTCCATCCCC CATTCCCAAG 
TCACGTCGAG TGTCTTTTGC AACTCCAATT TACCAGCAAG AACTGGCTGA TGACATTGAT 
CGTCGTAGTC CGGTTATTCG CACAAGTTCA CCTAGGTCAA AAGTCTTGAG TGGACAGTCA 
AAGTATATCA CTACACCAAC AAAAGGCGGC TATACAAGTC TGAGTCCACG TAACCTCCGT 
AGCCCTGGAT CCAAAAGCTC TAAAAAATGT CTGATTTCAG AGATGAGCCA GGAGCCACGT 
CCTATTCCTA AAGATTGTGT TTATCCAGCA CTTGTTGGCT GCTCTACTCC AGTAGAAGCT 
GTTCTGCCAC AGATTTCCTC CAACATGTGG CCGCGTGGCT TTGGCCAGCT TGTTCGAGCC 
AGGAACATTA AGACAGTAGG AGATCTGAGT GCTCTTACCC CTAATGAAAT CAAGTCTCTT 
CCTATTCGCT CACCTAAGCT GTCCAATGTG AGGAAAGCAC TTAAAACATA TCATGAACAA 
CAGAGAAAAG GACGTTCGGA TGACCTGAAG AGTTTTGACG AAATGGAGAA AATGACGTCT 
GAACCTGAAG AGATGGAGCT TCCTCAAAAC CAAGATGAGG AGCAAACATT AGGACCAGAG 
GCTCAAACAG CAGAATCAGA GACTCAAGAA GACAAAGCCT CAGAAGTGGA AGTAAATTCA 
GAAGTGGTGA CTGAAGAGAA CCAATCTCAT GATTTGCTCT CAGATGTTGT AGCTCTTGGT 
GAACGCTTTA CGCCTGAGGA ACTCGGTCAG CATTCAGCCA GTCAGTTGGG TCTGATGCAT 
GAACATCTGA GTGGAATGAT GAGGAGCATT GTGACCAATA TGCAGTCCCG CTTGCTCAGT 
AATCTGGACG ATAGCTTGCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074275.1
CDS	1..7044
Translation	MAAVPLSSTSLLPLLECLEDESAGLSAQTDAYITIANRLNGEEGRQFLPVVVKHFARLSKVHISSENEELCQAALQALGFCVFHSHIVSVIPANISENILSTLCNVVVKSKEKFTCTRALWVISKQNFPPEVASKMILKSLEAMQTREVQSILIDHESLNVIIRLLEQAPVPMAVGAVCWAKLVIPLVVHSANKVRLRAAAALELGMPLLLEKQQEVAAIVEPMMSSVLIPEMQKLFASKNETNMLKLWPLFVRLLGKLLHKGGAFINSLLYLEELGFRNSSPNIKKIAFIAWKSLIDNFALNPDILCSSKRLKLLMQPLSSIQVRTEALLLTKLEVWWYLAVKLGPNLSANFEQSPTTPARTSNPSNGTPNTPKSGIPSCSTPSTTPRMNLNSSMQAPQSYRSIQLLGLEMLLHWLVGPEVTVTAARENLQLSLEPLTHPLFTSPSTFSRHASTLISASKEGIITVGKHAPGNKKERQGSEVLTLFLQALQSILSSDALPPERALLLLEATVKGIPQKVLGSAAYQVANMDVLNGTPALFLILLFYKSNQLSSFLEDERFFSSLETLVSCGFCGPTSPLAFGEAVLGAVNGSVETVKNKKQLWRMWSMVVSPLTDTITQTNEVNQGDALEHNFSAIYSALMFPVIHLLPYSALPQTTQKAMISTWSRLYKAFARCSALVATAEENTCCEELCVKISASLDREALKSLSMLDAIAHILLIIVESMDFSPYTPQFQQKMKSPRTPMSWVRKRSRALGNLSTFHTLLIQTLEAFLTADHSEESAEPLNGIGSALVSILTSLFTNITLPSFLQEVLSSLTKPLARLYEQFSCCDDPSKLSPELGHKLEKLAADLLGCLQTHLTLHDDEFLTVLSPLLCVLFLHKSKQVCSVVTQFWNATFGNALTLSYPEPLKCVLNQAKQKTPLILPGFEVVDVPDDYSGTYMSESSQLEPQISGVKVTSVGKRESLLERAEELTGKSSVTTSKPVSVKLDFGSPKPLRREALEEEASEDFVFIPPEMKERVLTEHQKEVKRTKRVDIPALYNNLDASMDTTVFSQYTQSQEESMDTLPKGEKDHPVEAASVQLREVASNEVPTTDVKVQKVTSSDIETPDKATPNKDQTLEMDQSPSTGVSVDVSGLEKSALEGTSPNISSSSDVVSGTPPKPNSRRQSFITLEKYAEGKSTSPASPAFTGTLTRRSRRLEASQASTSNQNDSQPQIKKVDSQESQKMSSIDMSIQNEHASMEVKDEKSVGANPCEGTDEEDVVPDTQTQVSGDTLGIKSSPVSKAHAEAQGTPEKNSQSPNDSLASQEPRRSGRRRSKPVRPGQDAEEIKSKSQEQASQPADSAIINSQKPLIASPSSTLTGRRKKKVETNKMEDQLNNEEMKGDLSQSDSQISAADPPQTVGVHANKDLSQTELSPKSSPVTQSNSQSKERLPALCESESMLLGRQETKTQLEESTEKETNNDSQNASSAARQKHIQSTTSDSESDSQQIVRKRRSRRSETKLLEISGVPIKEDLNQTDSQTPTGVKGRAMRRRTVEGAESSKDSTSAVNTEELSAGKESSQELGRYRTRRSRGLLSPESMESENSDSREDGARPKKRLRKPPSTEVLPVASEPKVIEMVPEKDDVQSEVLMETSKDEADPENISNTSVSETKELEQNNKEEVPAPCGAPHEQIIMEKAASNNKIDTDLTDTDEKETVKAEGEEANESSQTGSEDFKKTDVLHKCPHTKGRGRARRRSKNCNCFSNNRDVFSQDSDFQESQDLKNEQVPLAANILNSQPDQSSPTTASEENKSSAIEEIPVADVPSLSALPADLPSEVSPSESPITKLGVIVSQLPEVEKEAQPGSSKPMKDSSKELVEEVNRLSSGSTGGQKHKGENRLENTSVSQEPVQSSTPQDTSPSQGRPVDDAPICQEQLESSHVQQEDAVNPAVMEREDLAVSEQRQEKAVLSEESDGKGLLDKEITLVEKEEEMQVNNQDLSVDVQEELPVVPQSSTSEACLDSPPKPKLLSALSGELEPGQSPTRSRSRVWSPSASPSTSILKKGQKRACEEETPSPIPKSRRVSFATPIYQQELADDIDRRSPVIRTSSPRSKVLSGQSKYITTPTKGGYTSLSPRNLRSPGSKSSKKCLISEMSQEPRPIPKDCVYPALVGCSTPVEAVLPQISSNMWPRGFGQLVRARNIKTVGDLSALTPNEIKSLPIRSPKLSNVRKALKTYHEQQRKGRSDDLKSFDEMEKMTSEPEEMELPQNQDEEQTLGPEAQTAESETQEDKASEVEVNSEVVTEENQSHDLLSDVVALGERFTPEELGQHSASQLGLMHEHLSGMMRSIVTNMQSRLLSNLDDSLP

Target ORF information:

RefSeq Version	NM_001080806.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio replication timing regulatory factor 1 (rif1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080806.1

ORF Insert Sequence:

ATGGCGGCAG TGCCTTTATC CAGTACCAGC CTCCTTCCTC TTCTGGAGTG TTTGGAAGAT 
GAATCAGCTG GGCTTTCAGC GCAGACGGAT GCCTACATCA CCATTGCAAA CCGACTTAAT 
GGAGAAGAAG GACGCCAGTT TCTGCCTGTG GTTGTTAAGC ATTTTGCACG TCTTAGTAAA 
GTTCACATCT CCAGTGAGAA TGAGGAGCTG TGTCAGGCTG CACTGCAGGC TTTGGGATTC 
TGTGTCTTCC ACTCACACAT AGTTTCAGTC ATACCAGCAA ATATTTCAGA GAATATATTG 
TCTACGCTCT GCAATGTTGT GGTAAAGTCC AAAGAGAAGT TTACGTGCAC TCGGGCATTG 
TGGGTCATTT CAAAACAGAA TTTCCCTCCA GAGGTGGCAT CCAAAATGAT TCTCAAGAGC 
CTAGAAGCGA TGCAGACCAG AGAGGTGCAG TCCATCCTCA TTGACCACGA GTCACTCAAT 
GTCATCATAA GGTTACTGGA GCAGGCCCCT GTTCCGATGG CTGTTGGAGC TGTTTGCTGG 
GCTAAACTTG TTATTCCACT GGTGGTTCAT TCGGCCAATA AAGTGCGTCT GCGAGCGGCA 
GCTGCCCTGG AGCTCGGCAT GCCTCTTCTT CTGGAGAAAC AGCAGGAAGT GGCAGCCATT 
GTTGAGCCAA TGATGTCTTC TGTGCTCATC CCTGAAATGC AGAAGCTCTT TGCGTCTAAG 
AACGAGACAA ACATGTTGAA ACTCTGGCCT TTGTTTGTAA GACTTCTGGG CAAGTTACTC 
CACAAAGGCG GTGCTTTCAT TAACTCCTTG CTGTATCTAG AGGAGCTGGG TTTCCGCAAC 
TCTTCTCCTA ATATTAAGAA GATTGCTTTC ATAGCTTGGA AGAGCCTCAT TGACAATTTT 
GCCCTTAATC CAGATATCCT GTGCAGTAGC AAGCGTCTGA AGCTCCTCAT GCAGCCGCTC 
AGCTCCATCC AAGTCAGGAC TGAAGCTCTT CTGCTTACCA AGCTGGAGGT CTGGTGGTAT 
CTTGCTGTCA AGCTCGGACC CAACCTGTCT GCTAACTTTG AACAGAGCCC GACTACACCT 
GCTCGAACCT CTAACCCAAG CAATGGCACA CCTAACACCC CTAAGTCAGG CATTCCCTCC 
TGCAGCACGC CGAGCACGAC TCCTCGCATG AATCTGAACA GCAGCATGCA GGCGCCTCAG 
TCCTATCGTT CCATTCAGCT CCTGGGACTA GAGATGCTCC TGCACTGGCT TGTGGGTCCA 
GAGGTCACAG TCACAGCTGC TCGAGAAAAC CTTCAGCTCA GCCTGGAGCC CCTGACGCAC 
CCGCTTTTTA CCAGCCCTTC CACCTTCAGC AGACATGCTT CTACCCTCAT ATCTGCCAGC 
AAAGAAGGCA TCATTACTGT AGGGAAGCAT GCTCCAGGAA ATAAAAAGGA GCGACAGGGT 
TCAGAGGTCC TCACACTTTT CCTGCAGGCA TTGCAGTCCA TTTTGTCATC AGACGCTCTG 
CCTCCTGAAC GTGCACTGCT TCTGTTGGAA GCCACTGTAA AGGGAATACC TCAGAAGGTG 
CTTGGTTCTG CAGCTTATCA AGTGGCAAAC ATGGACGTGT TAAACGGTAC TCCTGCACTT 
TTCCTAATAC TGCTGTTCTA TAAAAGTAAC CAGCTCTCAT CATTTCTTGA AGACGAAAGA 
TTTTTTTCAA GCTTGGAGAC CCTTGTAAGC TGTGGGTTTT GTGGACCCAC ATCGCCCTTG 
GCATTTGGAG AGGCGGTTTT AGGAGCAGTT AATGGGAGTG TGGAGACTGT TAAAAACAAG 
AAGCAGCTCT GGAGAATGTG GAGTATGGTT GTAAGTCCTC TGACGGACAC AATTACTCAG 
ACCAATGAAG TCAACCAAGG CGATGCTCTG GAGCATAACT TCAGTGCCAT TTACAGTGCG 
TTGATGTTCC CCGTCATCCA CCTGCTGCCT TATTCAGCCT TACCACAGAC CACTCAGAAG 
GCCATGATCA GCACATGGTC CAGGCTTTAC AAGGCTTTTG CCCGTTGCTC AGCTTTGGTT 
GCCACAGCAG AGGAGAATAC ATGCTGTGAA GAGTTGTGTG TCAAGATCTC TGCCTCTCTT 
GACCGTGAAG CTCTAAAAAG TCTGTCTATG CTAGATGCAA TAGCACACAT CTTGCTCATC 
ATCGTTGAGA GTATGGACTT TTCTCCATAT ACCCCTCAGT TCCAGCAGAA GATGAAATCT 
CCTCGTACAC CCATGAGTTG GGTACGCAAG AGAAGCAGAG CCCTCGGGAA TCTCTCAACC 
TTCCACACTC TTCTGATACA GACTCTAGAG GCTTTTCTCA CTGCAGACCA TTCAGAAGAA 
TCTGCAGAGC CTTTAAATGG AATTGGCTCT GCCTTGGTGT CAATCTTGAC CTCTCTTTTC 
ACTAATATTA CCCTTCCCTC ATTCTTACAA GAGGTGCTCT CCAGCCTCAC CAAACCACTT 
GCACGTCTTT ATGAGCAGTT CTCCTGTTGT GATGACCCAT CAAAGCTCAG TCCAGAACTA 
GGGCACAAGC TGGAGAAACT GGCCGCAGAC TTGCTAGGCT GCCTTCAGAC ACATTTGACT 
CTCCATGATG ATGAATTCCT CACTGTGTTG TCGCCCTTGC TTTGTGTACT TTTTTTGCAC 
AAGAGCAAAC AGGTTTGCTC TGTGGTCACT CAATTCTGGA ATGCCACTTT TGGAAATGCG 
CTCACCTTGA GTTACCCAGA ACCTCTGAAG TGTGTTTTAA ATCAGGCTAA GCAGAAGACT 
CCACTGATTT TACCTGGTTT TGAAGTTGTT GATGTACCAG ATGATTACAG TGGAACATAT 
ATGAGTGAGA GTTCTCAGCT GGAGCCTCAG ATTAGCGGGG TAAAAGTGAC CTCTGTGGGA 
AAGAGGGAAT CTCTTTTGGA AAGAGCAGAA GAGCTAACAG GAAAAAGTTC TGTAACCACA 
TCAAAACCAG TGTCTGTAAA GCTGGACTTT GGCTCCCCCA AGCCCTTAAG ACGAGAAGCT 
CTTGAGGAGG AAGCATCTGA AGATTTTGTT TTTATTCCAC CTGAGATGAA AGAGAGAGTC 
CTGACTGAGC ATCAGAAGGA AGTGAAAAGG ACTAAACGGG TTGACATTCC TGCCCTGTAC 
AATAATCTTG ATGCCTCCAT GGACACCACA GTCTTTTCAC AGTATACTCA GAGTCAGGAG 
GAGTCAATGG ACACATTGCC TAAAGGTGAG AAGGATCATC CTGTGGAAGC GGCTAGTGTT 
CAACTGAGGG AGGTTGCCTC AAATGAAGTA CCAACCACAG ATGTTAAAGT CCAAAAAGTC 
ACCAGTTCTG ATATAGAAAC TCCAGATAAA GCTACGCCGA ATAAAGATCA AACTTTGGAG 
ATGGATCAAT CACCATCTAC TGGTGTCTCA GTTGATGTGA GTGGGCTTGA GAAAAGCGCA 
TTGGAGGGGA CAAGTCCTAA TATTTCAAGT TCATCTGATG TGGTATCTGG CACACCTCCT 
AAACCTAACA GCAGGCGTCA GTCATTTATA ACCTTAGAAA AATATGCTGA AGGGAAATCG 
ACAAGTCCTG CAAGTCCTGC ATTTACAGGT ACGCTCACCC GGAGATCAAG AAGACTGGAG 
GCTTCACAGG CATCAACATC AAATCAAAAT GATTCTCAAC CTCAGATTAA AAAAGTGGAT 
TCTCAGGAAT CTCAAAAGAT GAGCAGCATT GACATGTCCA TTCAAAATGA GCATGCTAGC 
ATGGAAGTAA AAGATGAGAA ATCAGTGGGA GCTAATCCAT GTGAAGGTAC AGATGAGGAA 
GATGTTGTTC CAGATACCCA AACCCAAGTA TCTGGTGACA CATTGGGTAT TAAAAGCAGT 
CCTGTGTCTA AAGCCCATGC GGAGGCACAG GGGACTCCTG AAAAAAATTC ACAGAGTCCT 
AATGATTCTC TAGCTAGTCA AGAACCAAGA AGGTCTGGCA GAAGGCGAAG TAAACCTGTT 
CGTCCAGGGC AAGATGCAGA GGAAATAAAA AGTAAATCTC AAGAACAAGC CAGTCAACCT 
GCAGACTCTG CGATAATAAA CTCACAAAAG CCCCTTATTG CTTCACCTAG TAGTACTTTG 
ACTGGAAGAA GGAAAAAAAA GGTTGAGACC AATAAAATGG AAGACCAGCT GAACAATGAA 
GAAATGAAAG GAGATCTCAG CCAAAGCGAT TCACAGATTT CCGCTGCTGA CCCTCCACAG 
ACCGTAGGTG TGCATGCCAA TAAAGACTTA AGCCAGACTG AGTTATCTCC GAAGAGTTCT 
CCTGTAACCC AGTCAAATAG CCAAAGCAAA GAGAGACTGC CAGCTCTCTG CGAGTCTGAG 
AGTATGTTGT TAGGTAGACA GGAGACAAAA ACACAACTTG AAGAATCCAC AGAAAAGGAG 
ACAAATAATG ACTCTCAAAA CGCTTCATCT GCAGCGAGAC AGAAGCATAT ACAGTCTACC 
ACGTCTGACT CCGAGTCGGA TAGCCAGCAA ATTGTCCGTA AACGAAGATC GAGGAGATCT 
GAAACTAAGT TACTGGAGAT ATCTGGTGTG CCGATCAAAG AAGACTTGAA TCAGACAGAC 
TCCCAAACTC CAACAGGAGT GAAGGGAAGA GCTATGAGGA GGAGAACCGT AGAAGGTGCA 
GAGTCTAGTA AAGATTCCAC ATCAGCGGTA AATACAGAGG AGTTAAGTGC AGGCAAGGAG 
TCATCACAAG AGCTTGGTAG ATACAGAACT CGACGTTCTA GGGGTTTGTT GTCTCCTGAG 
AGTATGGAGT CTGAAAACTC TGATAGCCGA GAAGATGGGG CAAGGCCTAA AAAGAGGCTG 
CGAAAACCAC CATCAACAGA GGTGTTACCT GTTGCTTCAG AACCCAAAGT TATTGAGATG 
GTACCAGAAA AGGACGATGT TCAGTCAGAG GTTTTAATGG AGACGTCAAA GGATGAGGCA 
GATCCTGAAA ATATCTCGAA TACTTCAGTC AGTGAGACCA AAGAACTTGA GCAAAATAAT 
AAAGAGGAAG TCCCTGCCCC TTGTGGTGCA CCACATGAAC AGATTATCAT GGAAAAAGCA 
GCATCAAACA ACAAAATTGA CACTGACTTA ACTGATACAG ACGAAAAAGA AACCGTCAAG 
GCAGAGGGTG AAGAAGCTAA TGAATCTTCG CAAACTGGAT CAGAAGATTT TAAAAAGACA 
GATGTTTTGC ATAAATGCCC ACATACAAAG GGACGGGGCC GTGCACGTAG ACGATCTAAA 
AACTGCAACT GCTTTTCCAA CAACCGTGAT GTGTTTTCAC AGGACAGTGA CTTCCAAGAA 
TCACAAGATC TAAAAAATGA ACAGGTTCCA CTTGCTGCCA ACATCTTGAA CTCTCAACCT 
GATCAGTCAA GCCCAACAAC TGCATCAGAA GAAAACAAAA GCTCAGCAAT TGAAGAAATC 
CCTGTTGCTG ATGTTCCATC ACTTTCAGCC TTACCTGCTG ATCTCCCATC TGAGGTTTCG 
CCTTCTGAAA GTCCCATTAC AAAACTTGGG GTAATTGTTT CTCAGCTACC AGAAGTGGAG 
AAAGAAGCAC AGCCTGGATC ATCCAAACCA ATGAAGGATT CGAGCAAGGA GCTTGTAGAA 
GAAGTGAATC GATTAAGTTC TGGATCCACA GGTGGTCAAA AGCACAAAGG AGAAAATAGA 
TTAGAAAACA CTTCAGTAAG CCAAGAACCT GTCCAGAGTT CAACACCTCA AGACACCTCT 
CCAAGTCAAG GTCGTCCAGT GGACGATGCA CCAATATGCC AGGAGCAGCT AGAGTCTTCT 
CATGTCCAGC AGGAAGATGC AGTTAACCCA GCAGTGATGG AAAGAGAAGA CCTTGCTGTG 
TCAGAGCAGA GACAAGAGAA GGCTGTGTTG TCTGAAGAGT CGGATGGAAA AGGTTTGCTT 
GACAAGGAAA TTACTCTTGT TGAAAAGGAA GAAGAGATGC AGGTTAATAA CCAGGATCTT 
TCTGTTGATG TCCAAGAAGA GTTACCTGTA GTGCCCCAGA GTAGCACATC TGAAGCCTGC 
TTGGATTCTC CACCCAAGCC AAAACTGTTA AGCGCACTCA GTGGTGAACT GGAGCCTGGC 
CAGAGTCCAA CTAGAAGTCG GTCTCGAGTC TGGTCACCAT CTGCTTCTCC GTCTACCAGT 
ATTTTGAAGA AGGGTCAGAA GAGAGCATGT GAAGAGGAGA CTCCATCCCC CATTCCCAAG 
TCACGTCGAG TGTCTTTTGC AACTCCAATT TACCAGCAAG AACTGGCTGA TGACATTGAT 
CGTCGTAGTC CGGTTATTCG CACAAGTTCA CCTAGGTCAA AAGTCTTGAG TGGACAGTCA 
AAGTATATCA CTACACCAAC AAAAGGCGGC TATACAAGTC TGAGTCCACG TAACCTCCGT 
AGCCCTGGAT CCAAAAGCTC TAAAAAATGT CTGATTTCAG AGATGAGCCA GGAGCCACGT 
CCTATTCCTA AAGATTGTGT TTATCCAGCA CTTGTTGGCT GCTCTACTCC AGTAGAAGCT 
GTTCTGCCAC AGATTTCCTC CAACATGTGG CCGCGTGGCT TTGGCCAGCT TGTTCGAGCC 
AGGAACATTA AGACAGTAGG AGATCTGAGT GCTCTTACCC CTAATGAAAT CAAGTCTCTT 
CCTATTCGCT CACCTAAGCT GTCCAATGTG AGGAAAGCAC TTAAAACATA TCATGAACAA 
CAGAGAAAAG GACGTTCGGA TGACCTGAAG AGTTTTGACG AAATGGAGAA AATGACGTCT 
GAACCTGAAG AGATGGAGCT TCCTCAAAAC CAAGATGAGG AGCAAACATT AGGACCAGAG 
GCTCAAACAG CAGAATCAGA GACTCAAGAA GACAAAGCCT CAGAAGTGGA AGTAAATTCA 
GAAGTGGTGA CTGAAGAGAA CCAATCTCAT GATTTGCTCT CAGATGTTGT AGCTCTTGGT 
GAACGCTTTA CGCCTGAGGA ACTCGGTCAG CATTCAGCCA GTCAGTTGGG TCTGATGCAT 
GAACATCTGA GTGGAATGAT GAGGAGCATT GTGACCAATA TGCAGTCCCG CTTGCTCAGT 
AATCTGGACG ATAGCTTGCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_005167575.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167632.1 Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X1, mRNA.

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CloneID	ODa15091
Clone ID Related Accession (Same CDS sequence)	XM_005167575.3 , XM_005167575.4 , XM_009304632.2
Accession Version	XM_005167575.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	telomere-associated protein RIF1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167575.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CAGTGCCTTT ATCCAGTACC AGCCTCCTTC CTCTTCTGGA GTGTTTGGAA 
GATGAATCAG CTGGGCTTTC AGCGCAGACG GATGCCTACA TCACCATTGC AAACCGACTT 
AATGGAGAAG AAGGACGCCA GTTTCTGCCT GTGGTTGTTA AGCATTTTGC ACGTCTTAGT 
AAAGTGCTTT TGGTTCACAT CTCCAGTGAG AATGAGGAGC TGTGTCAGGC TGCACTGCAG 
GCTTTGGGAT TCTGTGTCTT CCACTCACAC ATAGTTTCAG TCATACCAGC AAATATTTCA 
GAGAATATAT TGTCTACGCT CTGCAATGTT GTGGTAAAGT CCAAAGAGAA GTTTACGTGC 
ACTCGGGCAT TGTGGGTCAT TTCAAAACAG AATTTCCCTC CAGAGGTGGC ATCCAAAATG 
GCCCCAGAGA TTCTCAAGAG CCTAGAAGCG ATGCAGACCA GAGAGGTGCA GTCCATCCTC 
ATTGACCACG AGTCACTCAA TGTCATCATA AGGTTACTGG AGCAGGCCCC TGTTCCGATG 
GCTGTTGGTG CTGTTTGCTG GGCTAAACTT GTTATTCCTC TGGTGGTTCA TTCGGCCAAT 
AAAGTGCGTC TGCGAGCGGC AGCTGCCCTG GAGCTCGGCA TGCCTCTTCT TCTGGAGAAA 
CAGCAGGAAG TGGCAGCCAT TGTTGAGCCA ATGATGTCTT CTGTGCTCAT CCCTGAAATG 
CAGAAGCTCT TTGCGTCTAA GAACGAGACA AACATGTTGA AACTCTGGCC TTTGTTTGTA 
AGACTTCTGG GCAAGTTACT CCACAAAGGC GGTGCTTTCA TTAACTCCTT GCTGTATCTA 
GAGGAGCTGG GTTTCCGCAA CTCTTCTCCT AATATTAAGA AGATTGCTTT CATAGCTTGG 
AAGAGCCTCA TTGACAATTT TGCCCTTAAT CCAGATATCC TGTGCAGTAG CAAGCGTCTG 
AAGCTCCTCA TGCAGCCGCT CAGCTCCATC CAAGTCAGGA CTGAAGCTCT TCTGCTTACC 
AAGCTGGAGG TCTGGTGGTA TCTTGCTGTC AAGCTCGGAC CCAACCTGTC TGCTAACTTT 
GAACAGGTTG GCGTGCCTCT ATTGCACAGC ACACTTCCTT CTGATTCTCC ACTTCAGAGC 
CCGACTACAC CTGCTCGAAC CTCTAACCCA AGCAACGGCA CACCTAACAC CCCTAAGTCA 
GGCATTCCCT CCTGCAGCAC GCCGAGCACG ACTCCTCGCA TGAATCTGAA CAGCAGCATG 
CAGGCGCCTC AGTCCTATCG TTCCATTCAG CTCCTGGGAC TAGAGATGCT CCTGCACTGG 
CTTGTGGGTC CAGAGGTCAC AGTCACAGCT GCTCGAGAAA ACCTTCAGCT CAGCCTGGAG 
CCCCTGACGC ACCCGCTTTT TACCAGCCCT TCCACCTTCA GCAGACATGC TTCTACCCTC 
ATATCTGCCA GCAAAGAAGG CATCATTACT GTAGGGAAGC ATGCTCCAGA AGCACTTCTT 
AATCTTATTT GGAGCAACCT GATCATTTCT GTCAGTGCAG CAATTGAAGC TGGAAATAAA 
AAGGAGCGAC AGGGTTCAGA GGTCCTCACA CTTTTCCTGC AGGCATTGCA GTCCATTTTG 
TCATCAGACG CTCTGCCTCC TGAACGTGCA CTGCTTCTGT TGGAAGCCAC TGTAAAGGGA 
ATACCTCAGA AGGTGCTTGG TTCTGCAGCT TATCAAGTGG CAAACATGGA CGTGTTAAAC 
GGTACTCCTG CACTTTTCCT AATACTGCTG TTCTATAAAA GTAACCAGCT CTCATCATTT 
CTTGAAGACG AAAGATTTTT TTCAAGCTTG GAGACCCTTG TAAGCTGTGG GTTTTGTGGA 
CCCACATCGC CCTTGGCATT TGGAGAGGCG GTTTTAGGAG CAGTTAATGG GAGTGTGGAG 
ACTGTTAAAA ACAAGAAGCA GCTCTGGAGA ATGTGGAGTA TGGTTGTAAG TCCTCTGACG 
GACACAATTA CTCAGACCAA TGAAGTCAAC CAAGGCGATG CTCTGGAGCA TAACTTCAGT 
GCCATTTACA GTGCGTTGAT GTTCCCCGTC ATCCACCTGC TGCCTTATTC AGCCTTACCA 
CAGACCACTC AGAAGGCCAT GATCAGCACA TGGTCCAGGC TTTACAAGGC TTTTGCCCGT 
TGCTCAGCTT TGGTTGCCAC AGCAGAGGAG AATACATGCT GTGAAGAGTT GTGTGTCAAG 
ATCTCTGCCT CTCTTGACCG TGAAGCTCTA AAAAGTCTGT CTATGCTAGA TGCAATAGCA 
CACATCTTGC TCATCATCGT TGAGAGTATG GACTTTTCTC CATATACCCC TCAGTTCCAG 
CAGAAGATGA AATCTCCTCG TACACCCATG AGTTGGGTAC GCAAGAGAAG CAGAGCCCTC 
GGGAATCTCT CAACCTTCCA CACTCTTCTG ATACAGACTC TAGAGGCTTT TCTCACTGCA 
GACCATTCAG AAGAATCTGC AGAGCCTTTA AATGGAATTG GCTCTGCCTT GGTGTCAATC 
TTGACCTCTC TTTTCACTAA TATTACCCTT CCCTCATTCT TACAAGAGGT GCTCTCCAGC 
CTCACCAAAC CACTTGCACG TCTTTATGAG CAGTTCTCCT GTTGTGATGA CCCATCAAAG 
CTCAGTCCAG AACTAGGGCA CAAGCTGGAG AAACTGGCCG CAGACTTGCT AGGCTGCCTT 
CAGACACATT TGACTCTCCA TGATGATGAA TTCCTCACTG TGTTGTCGCC CTTGCTTTGT 
GTACTTTTTT TGCACAAGAG CAAACAGGTT TGCTCTGTGG TCACTCAATT CTGGAATGCC 
ACTTTTGGAA ATGCGCTCAC CTTGAGTTAC CCAGAACCTC TGAAGTGTGT TTTAAATCAG 
GCTAAGCAGA AGACTCCACT GATTTTACCT GGTTTTGAAG TTGTTGATGT ACCAGATGAT 
TACAGTGGAA CATATATGAG TGAGAGTTCT CAGCTGGAGC CTCAGATTAG CGGGGTAAAA 
GTGACCTCTG TGGGAAAGAG GGACTCTCTT TTGGCAAGAG CAGAAGAGCT AACAGGAAAA 
AGTTCTGTAA CCACATCAAA ACCAGTGTCT GTAAAGCTGG ACTTTGGCTC CCCCAAGCCC 
TTAAGACGAG AAGCTCTTGA GGAGGAAGCA TCTGAAGATT TTGTTTTTAT TCCACCTGAG 
ATGAAAGAGA GAGTCCTGAC TGAGCATCAG AAGGAAGTGA AAAGGACTAA ACGGGTTGAC 
ATTCCTGCCC TGTACAATAA TCTTGATGCC TCCATGGACA CCACAGTCTT TTCACAGTAT 
ACTCAGAGTC AGGAGGAGTC AATGGACACA TTGCCTAAAG GTGAGAAGGA TCATCCTGTG 
GAAGCGGCTA GTGTTCAACT GAGGGAGGTT GCCTCAAATG AAGTACCAAC CACAGATGTT 
AAAGTCCAAA AAGTCACCAG TTCTGATATA GAAACTCCAG ATAAAGCTAC GCCGAATAAA 
GATCAAACTT TGGAGATGGA TCAATCACCA TCTACTGGTG TCTCAGTTGA TGTGAGTGGG 
CTTGAGAAAA GCGCATTGGA GGGGACAAGT CCTAATATTT CAAGTTCATC TGATGTGGTA 
TCTGGCACAC CTCCTAAACC TAACAGCAGG CGTCAGTCAT TTATAACCTT AGAAAAATAT 
GCTGAAGGGA AATCGACAAG TCCTGCAAGT CCTGCATTTA CAGGTACGCT CACCCGGAGA 
TCAAGAAGAC TGGAGGCTTC ACAGGCATCA ACATCAAATC AAAATGATTC TCAACCTCAG 
ATTAAAAAAG TGGATTCTCA GGAATCTCAA AAGATGAGCA GCATTGACAT GTCCATTCAA 
AATGAGCATG CTAGCATGGA AGTAAAAGAT GAGAAGTCAG TGGGAGCTAA TCCATGTGAA 
GGTACAGATG AGGAAGATGT TGTTCCAGAT ACCCAAACCC AAGTATCTGG TGACACATTG 
GGTATTAAAA GCAGTCCTGT GTCTAAAGCC CATGCGGAGG CACAGGGGAC TCCTGAAAAA 
AATTCACAGA GTCCTAATGA TTCTCTAGCT AGTCAAGAAC CAAGAAGGTC TGGCAGAAGG 
CGAAGTAAAC CTGTTCGTCC AGGGCAAGAT GCAGAGGAAA TAAAAAGTAA ATCTCAAGAA 
CAAGCCAGTC AACCTGCAGA CTCTGCGATA ATAAACTCAC AAAAGCCCCT TCTTGCTTCA 
CCTAGTAGTA CTTTGACTGG AAGAAGGAAA AAAAAGGTTG AGACCAATAA AATGGAAGAC 
CAGCTGAACA ATGAAGAAAT GAAAGGAGAT CTCAGCCAAA GCGATTCACA GATTTCCGCT 
GCTGACCCTC CACAGACAGT AGGTGTGCAT GCCAATAAAG ACTTAAGCCA GACTGAGTTA 
TCTCCGAAGA GTTCTCCTGT AACCCAGTCA AATAGCCAAA GCAAAGAGAG ACTGCCAGCT 
CTCTGCGAGT CTGAGAGTAT GTCGTTAGGT AGACAGGAGA CAAAAACACA ACTTGAAGAA 
TCCACAGAAA AGGAGACAAA TAATGACTCT CAAAACGCTT CATCTGCAGC GAGACCGAAG 
CATATACAGT CTACCACGTC TGACTCCGAG TCGGATAGCC AGCAAATTGT CCGTAAACGA 
AGATCGAGGA GATCTGAAAC TAAGTTACTG GAGATATCTG GTGTGCCGAT CAAAGAAGAC 
TTGAATCAGA CAGACTCCCA AACTCCAACA GGAGTGAAGG GAAGAGCTAT GAGGAGGAGA 
ACCGTAGAAG GTGCAGAGTC TAGTAAAGAT TCCACATCAG CGGTAAATAC AGAGGAGTTA 
AGTGCAGGCA AGGAGTCATC ACAAGAGCTT GGTAGATACA GAACTCGACG TTCTAGGGGT 
TTGTTGTCTC CTGAGAGTAT GGAGTCTGAA AACTCTGATA GCCGAGAAGA TGGGGCAAGG 
CCTAAAAAGA GGCTGCGAAA ACCACCATCA ACAGAGGTGT TACCTGTTGC TTCAGAACCC 
AAAGTTATTG AGATGGTACC AGAAAAGGAC GATGTTCAGT CAGAGGTTTT AATGGAGACG 
TCAAAGGATG AGGCAGATCC TGAAAATATC TCGAATACTT CAGTCAGTGA AACCAAAGAA 
CTTGAGCAAA AGAATAAAGA GGAAGTCCCT GCCCCTTGTG ATGCACCACA TGAACAGATT 
ATCACGGAAA AAGCAGCATC AAACAACAAA ATTGACACTG ACTTAACTGA TACAGACGAA 
AAAGAAACCG TCAAGGCAGA GGGTGAAGAA GCTAATGAAT CTTCGCAAAC TGGATCAGAA 
GATTTTAAAA AGACAGATGT TTTGCATAAA TGCCCACATA CAAAGGGACG GGGCCGTGCA 
CGTAGACGAT CTAAAAACTG CAACTGCTTT TCCAACAACC GTGATGTGTT TTCACAGGAC 
AGTGACTTCC AAGAATCACA AGATCTAAAA AATGAACAGG TTCCACTTGC TGCCAACATC 
TTGAACTCTC AACCTGATCA GTCAAGCCCA TCAACTGCAT CAGAAGAAAA CAAAAGCTCA 
GCAATTGAAG AAGTCCCTGT TGCGGATGTT CCATCACTTT CAGCCTTATC AGCTGATCTC 
CCATCTGAGG TTTCACCTTC TGAAAGTCCC ATTACAAAAC TTGGGGTAAT TGTTTCTCAG 
CTACCAGAAG TGGAGAAAGA AGCACAGCCT GGATCATCCA AACCAATGAA GGATTCGAGC 
AAGGAGCTTG TAGAAGAAGT GAATCGATTA AGTTCTGGAT CCACAGGTGG TCAAAAGCAC 
AAAGGAGAAA ATAGATTAGA AAACACTTCA GTAAGCCAAG AACCTGTCCA GAGTTCAACA 
CCTCAAGACA CGTCTCCAAG TCAAGGTCGT CCAGTGGACG ATGCACCAAT ATGCCAGGAG 
CAGTTAGAGT CTTCTCATGT CCAGCAGGAA GATGCAGTTA ACCCAGCAGT GATGGAAAGA 
GAAGACCTTG CTGTGTCAGA GCAGAGACAA GAGAAGGCTG TGTTGTCTGA AGAGTTAGAT 
GGAAAAGGTT TGCTTGACAA GGAAACTACT CTTGTTGAAA AGGAAGAAGA GATGCAGGTT 
AATAACCAGG ATCTTCCTGT GGATGTCCAA GAAGAGTTAC CTGTAGTGTC CCAGAGTAGC 
ACATCTGAAG CCTGCTTGGA TTCTCCACCC AAGCCAAAAC TGTTAAGCGC ACTCAGTGGT 
GAACTAGAGC CTGGCCAGAG TCCAACTAGA AGTCGGTCTC GAGTCTGGTC ACCATCTGCT 
TCTCCGTCTA CCAGTATTCT GAAGAAGGGT CAGAAGAGAG CATGTGAAGA GGAGACTCCA 
TCCCCCATTC CCAAGTCACG TCGAGTGTCT TTTGCAACTC CAATTTACCA GCAAGAACTG 
GCTGATGACA TTGATCGTCG TAGTCCGGTT ATTCGCACAA GTTCACCTAG GTCAAAAGTC 
TTGAGTGGAC AGTCAAAGTA TATCACTACA CCAACAAAAG GTGGCTATAC AAGTCTGAGT 
CCACGTAACC TCCGTAGCCC TGGATCCAAA AGCTCTAAAA AATGTCTGAT TTCAGAGATG 
AGCCAGGAGC CACGTCCTAT TCCTAAAGAT TGTGTTTATC CAGCACTTGT TGGCTGCTCT 
ACTCCAGTAG AAGCTGTTCT GCCACAGATT TCCTCCAACA TGTGGCCGCG TGGCTTCGGC 
CAGCTTGTTC GAGCCAGGAA CATTAAGACA GTAGGAGATC TGAGTGCTCT TACCCCTAAT 
GAAATCAAGT CTCTTCCTAT TCGCTCACCT AAGCTGTCCA ATGTGAGGAA AGCACTTAAA 
ACATATCATG AACAACAGAG AAAAGGACGT TCGGATGACC TGAAGAGTTT TGACGAAATG 
GAGAAAATGA CGTCTGAACC TGAAGAGATG GAGCTTCCTC AAAACCAAGA TGAGGAGCAA 
ACATCAGGAC CAGAGGCTCA AACAGCAGAA TCAGAGACTC AAGAAGACAA AGCCTCAGAA 
GTGGAAGTAA ATTCAGAAGT GGTGACTGAA GAGAACCAAT CTCATGATTT GCTCTCAGAT 
ATTGTAGCTC TTGGTGAACG CTTTACGCCT GAGGAACTCA GTCAGCATTC AGCCAGTCAG 
TTGGGTCTGA TGCATGAACA TCTGAGTGGA ATGATGAGGA GCATTGTGAC CAATCTGCAG 
TCCCGCTTGC TCAGTAATCT GGACGATAGC TTGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167632.1
CDS	183..7361
Translation	MMAAVPLSSTSLLPLLECLEDESAGLSAQTDAYITIANRLNGEEGRQFLPVVVKHFARLSKVLLVHISSENEELCQAALQALGFCVFHSHIVSVIPANISENILSTLCNVVVKSKEKFTCTRALWVISKQNFPPEVASKMAPEILKSLEAMQTREVQSILIDHESLNVIIRLLEQAPVPMAVGAVCWAKLVIPLVVHSANKVRLRAAAALELGMPLLLEKQQEVAAIVEPMMSSVLIPEMQKLFASKNETNMLKLWPLFVRLLGKLLHKGGAFINSLLYLEELGFRNSSPNIKKIAFIAWKSLIDNFALNPDILCSSKRLKLLMQPLSSIQVRTEALLLTKLEVWWYLAVKLGPNLSANFEQVGVPLLHSTLPSDSPLQSPTTPARTSNPSNGTPNTPKSGIPSCSTPSTTPRMNLNSSMQAPQSYRSIQLLGLEMLLHWLVGPEVTVTAARENLQLSLEPLTHPLFTSPSTFSRHASTLISASKEGIITVGKHAPEALLNLIWSNLIISVSAAIEAGNKKERQGSEVLTLFLQALQSILSSDALPPERALLLLEATVKGIPQKVLGSAAYQVANMDVLNGTPALFLILLFYKSNQLSSFLEDERFFSSLETLVSCGFCGPTSPLAFGEAVLGAVNGSVETVKNKKQLWRMWSMVVSPLTDTITQTNEVNQGDALEHNFSAIYSALMFPVIHLLPYSALPQTTQKAMISTWSRLYKAFARCSALVATAEENTCCEELCVKISASLDREALKSLSMLDAIAHILLIIVESMDFSPYTPQFQQKMKSPRTPMSWVRKRSRALGNLSTFHTLLIQTLEAFLTADHSEESAEPLNGIGSALVSILTSLFTNITLPSFLQEVLSSLTKPLARLYEQFSCCDDPSKLSPELGHKLEKLAADLLGCLQTHLTLHDDEFLTVLSPLLCVLFLHKSKQVCSVVTQFWNATFGNALTLSYPEPLKCVLNQAKQKTPLILPGFEVVDVPDDYSGTYMSESSQLEPQISGVKVTSVGKRDSLLARAEELTGKSSVTTSKPVSVKLDFGSPKPLRREALEEEASEDFVFIPPEMKERVLTEHQKEVKRTKRVDIPALYNNLDASMDTTVFSQYTQSQEESMDTLPKGEKDHPVEAASVQLREVASNEVPTTDVKVQKVTSSDIETPDKATPNKDQTLEMDQSPSTGVSVDVSGLEKSALEGTSPNISSSSDVVSGTPPKPNSRRQSFITLEKYAEGKSTSPASPAFTGTLTRRSRRLEASQASTSNQNDSQPQIKKVDSQESQKMSSIDMSIQNEHASMEVKDEKSVGANPCEGTDEEDVVPDTQTQVSGDTLGIKSSPVSKAHAEAQGTPEKNSQSPNDSLASQEPRRSGRRRSKPVRPGQDAEEIKSKSQEQASQPADSAIINSQKPLLASPSSTLTGRRKKKVETNKMEDQLNNEEMKGDLSQSDSQISAADPPQTVGVHANKDLSQTELSPKSSPVTQSNSQSKERLPALCESESMSLGRQETKTQLEESTEKETNNDSQNASSAARPKHIQSTTSDSESDSQQIVRKRRSRRSETKLLEISGVPIKEDLNQTDSQTPTGVKGRAMRRRTVEGAESSKDSTSAVNTEELSAGKESSQELGRYRTRRSRGLLSPESMESENSDSREDGARPKKRLRKPPSTEVLPVASEPKVIEMVPEKDDVQSEVLMETSKDEADPENISNTSVSETKELEQKNKEEVPAPCDAPHEQIITEKAASNNKIDTDLTDTDEKETVKAEGEEANESSQTGSEDFKKTDVLHKCPHTKGRGRARRRSKNCNCFSNNRDVFSQDSDFQESQDLKNEQVPLAANILNSQPDQSSPSTASEENKSSAIEEVPVADVPSLSALSADLPSEVSPSESPITKLGVIVSQLPEVEKEAQPGSSKPMKDSSKELVEEVNRLSSGSTGGQKHKGENRLENTSVSQEPVQSSTPQDTSPSQGRPVDDAPICQEQLESSHVQQEDAVNPAVMEREDLAVSEQRQEKAVLSEELDGKGLLDKETTLVEKEEEMQVNNQDLPVDVQEELPVVSQSSTSEACLDSPPKPKLLSALSGELEPGQSPTRSRSRVWSPSASPSTSILKKGQKRACEEETPSPIPKSRRVSFATPIYQQELADDIDRRSPVIRTSSPRSKVLSGQSKYITTPTKGGYTSLSPRNLRSPGSKSSKKCLISEMSQEPRPIPKDCVYPALVGCSTPVEAVLPQISSNMWPRGFGQLVRARNIKTVGDLSALTPNEIKSLPIRSPKLSNVRKALKTYHEQQRKGRSDDLKSFDEMEKMTSEPEEMELPQNQDEEQTSGPEAQTAESETQEDKASEVEVNSEVVTEENQSHDLLSDIVALGERFTPEELSQHSASQLGLMHEHLSGMMRSIVTNLQSRLLSNLDDSLP

Target ORF information:

RefSeq Version	XM_005167575.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167575.3

ORF Insert Sequence:

ATGATGGCGG CAGTGCCTTT ATCCAGTACC AGCCTCCTTC CTCTTCTGGA GTGTTTGGAA 
GATGAATCAG CTGGGCTTTC AGCGCAGACG GATGCCTACA TCACCATTGC AAACCGACTT 
AATGGAGAAG AAGGACGCCA GTTTCTGCCT GTGGTTGTTA AGCATTTTGC ACGTCTTAGT 
AAAGTGCTTT TGGTTCACAT CTCCAGTGAG AATGAGGAGC TGTGTCAGGC TGCACTGCAG 
GCTTTGGGAT TCTGTGTCTT CCACTCACAC ATAGTTTCAG TCATACCAGC AAATATTTCA 
GAGAATATAT TGTCTACGCT CTGCAATGTT GTGGTAAAGT CCAAAGAGAA GTTTACGTGC 
ACTCGGGCAT TGTGGGTCAT TTCAAAACAG AATTTCCCTC CAGAGGTGGC ATCCAAAATG 
GCCCCAGAGA TTCTCAAGAG CCTAGAAGCG ATGCAGACCA GAGAGGTGCA GTCCATCCTC 
ATTGACCACG AGTCACTCAA TGTCATCATA AGGTTACTGG AGCAGGCCCC TGTTCCGATG 
GCTGTTGGTG CTGTTTGCTG GGCTAAACTT GTTATTCCTC TGGTGGTTCA TTCGGCCAAT 
AAAGTGCGTC TGCGAGCGGC AGCTGCCCTG GAGCTCGGCA TGCCTCTTCT TCTGGAGAAA 
CAGCAGGAAG TGGCAGCCAT TGTTGAGCCA ATGATGTCTT CTGTGCTCAT CCCTGAAATG 
CAGAAGCTCT TTGCGTCTAA GAACGAGACA AACATGTTGA AACTCTGGCC TTTGTTTGTA 
AGACTTCTGG GCAAGTTACT CCACAAAGGC GGTGCTTTCA TTAACTCCTT GCTGTATCTA 
GAGGAGCTGG GTTTCCGCAA CTCTTCTCCT AATATTAAGA AGATTGCTTT CATAGCTTGG 
AAGAGCCTCA TTGACAATTT TGCCCTTAAT CCAGATATCC TGTGCAGTAG CAAGCGTCTG 
AAGCTCCTCA TGCAGCCGCT CAGCTCCATC CAAGTCAGGA CTGAAGCTCT TCTGCTTACC 
AAGCTGGAGG TCTGGTGGTA TCTTGCTGTC AAGCTCGGAC CCAACCTGTC TGCTAACTTT 
GAACAGGTTG GCGTGCCTCT ATTGCACAGC ACACTTCCTT CTGATTCTCC ACTTCAGAGC 
CCGACTACAC CTGCTCGAAC CTCTAACCCA AGCAACGGCA CACCTAACAC CCCTAAGTCA 
GGCATTCCCT CCTGCAGCAC GCCGAGCACG ACTCCTCGCA TGAATCTGAA CAGCAGCATG 
CAGGCGCCTC AGTCCTATCG TTCCATTCAG CTCCTGGGAC TAGAGATGCT CCTGCACTGG 
CTTGTGGGTC CAGAGGTCAC AGTCACAGCT GCTCGAGAAA ACCTTCAGCT CAGCCTGGAG 
CCCCTGACGC ACCCGCTTTT TACCAGCCCT TCCACCTTCA GCAGACATGC TTCTACCCTC 
ATATCTGCCA GCAAAGAAGG CATCATTACT GTAGGGAAGC ATGCTCCAGA AGCACTTCTT 
AATCTTATTT GGAGCAACCT GATCATTTCT GTCAGTGCAG CAATTGAAGC TGGAAATAAA 
AAGGAGCGAC AGGGTTCAGA GGTCCTCACA CTTTTCCTGC AGGCATTGCA GTCCATTTTG 
TCATCAGACG CTCTGCCTCC TGAACGTGCA CTGCTTCTGT TGGAAGCCAC TGTAAAGGGA 
ATACCTCAGA AGGTGCTTGG TTCTGCAGCT TATCAAGTGG CAAACATGGA CGTGTTAAAC 
GGTACTCCTG CACTTTTCCT AATACTGCTG TTCTATAAAA GTAACCAGCT CTCATCATTT 
CTTGAAGACG AAAGATTTTT TTCAAGCTTG GAGACCCTTG TAAGCTGTGG GTTTTGTGGA 
CCCACATCGC CCTTGGCATT TGGAGAGGCG GTTTTAGGAG CAGTTAATGG GAGTGTGGAG 
ACTGTTAAAA ACAAGAAGCA GCTCTGGAGA ATGTGGAGTA TGGTTGTAAG TCCTCTGACG 
GACACAATTA CTCAGACCAA TGAAGTCAAC CAAGGCGATG CTCTGGAGCA TAACTTCAGT 
GCCATTTACA GTGCGTTGAT GTTCCCCGTC ATCCACCTGC TGCCTTATTC AGCCTTACCA 
CAGACCACTC AGAAGGCCAT GATCAGCACA TGGTCCAGGC TTTACAAGGC TTTTGCCCGT 
TGCTCAGCTT TGGTTGCCAC AGCAGAGGAG AATACATGCT GTGAAGAGTT GTGTGTCAAG 
ATCTCTGCCT CTCTTGACCG TGAAGCTCTA AAAAGTCTGT CTATGCTAGA TGCAATAGCA 
CACATCTTGC TCATCATCGT TGAGAGTATG GACTTTTCTC CATATACCCC TCAGTTCCAG 
CAGAAGATGA AATCTCCTCG TACACCCATG AGTTGGGTAC GCAAGAGAAG CAGAGCCCTC 
GGGAATCTCT CAACCTTCCA CACTCTTCTG ATACAGACTC TAGAGGCTTT TCTCACTGCA 
GACCATTCAG AAGAATCTGC AGAGCCTTTA AATGGAATTG GCTCTGCCTT GGTGTCAATC 
TTGACCTCTC TTTTCACTAA TATTACCCTT CCCTCATTCT TACAAGAGGT GCTCTCCAGC 
CTCACCAAAC CACTTGCACG TCTTTATGAG CAGTTCTCCT GTTGTGATGA CCCATCAAAG 
CTCAGTCCAG AACTAGGGCA CAAGCTGGAG AAACTGGCCG CAGACTTGCT AGGCTGCCTT 
CAGACACATT TGACTCTCCA TGATGATGAA TTCCTCACTG TGTTGTCGCC CTTGCTTTGT 
GTACTTTTTT TGCACAAGAG CAAACAGGTT TGCTCTGTGG TCACTCAATT CTGGAATGCC 
ACTTTTGGAA ATGCGCTCAC CTTGAGTTAC CCAGAACCTC TGAAGTGTGT TTTAAATCAG 
GCTAAGCAGA AGACTCCACT GATTTTACCT GGTTTTGAAG TTGTTGATGT ACCAGATGAT 
TACAGTGGAA CATATATGAG TGAGAGTTCT CAGCTGGAGC CTCAGATTAG CGGGGTAAAA 
GTGACCTCTG TGGGAAAGAG GGACTCTCTT TTGGCAAGAG CAGAAGAGCT AACAGGAAAA 
AGTTCTGTAA CCACATCAAA ACCAGTGTCT GTAAAGCTGG ACTTTGGCTC CCCCAAGCCC 
TTAAGACGAG AAGCTCTTGA GGAGGAAGCA TCTGAAGATT TTGTTTTTAT TCCACCTGAG 
ATGAAAGAGA GAGTCCTGAC TGAGCATCAG AAGGAAGTGA AAAGGACTAA ACGGGTTGAC 
ATTCCTGCCC TGTACAATAA TCTTGATGCC TCCATGGACA CCACAGTCTT TTCACAGTAT 
ACTCAGAGTC AGGAGGAGTC AATGGACACA TTGCCTAAAG GTGAGAAGGA TCATCCTGTG 
GAAGCGGCTA GTGTTCAACT GAGGGAGGTT GCCTCAAATG AAGTACCAAC CACAGATGTT 
AAAGTCCAAA AAGTCACCAG TTCTGATATA GAAACTCCAG ATAAAGCTAC GCCGAATAAA 
GATCAAACTT TGGAGATGGA TCAATCACCA TCTACTGGTG TCTCAGTTGA TGTGAGTGGG 
CTTGAGAAAA GCGCATTGGA GGGGACAAGT CCTAATATTT CAAGTTCATC TGATGTGGTA 
TCTGGCACAC CTCCTAAACC TAACAGCAGG CGTCAGTCAT TTATAACCTT AGAAAAATAT 
GCTGAAGGGA AATCGACAAG TCCTGCAAGT CCTGCATTTA CAGGTACGCT CACCCGGAGA 
TCAAGAAGAC TGGAGGCTTC ACAGGCATCA ACATCAAATC AAAATGATTC TCAACCTCAG 
ATTAAAAAAG TGGATTCTCA GGAATCTCAA AAGATGAGCA GCATTGACAT GTCCATTCAA 
AATGAGCATG CTAGCATGGA AGTAAAAGAT GAGAAGTCAG TGGGAGCTAA TCCATGTGAA 
GGTACAGATG AGGAAGATGT TGTTCCAGAT ACCCAAACCC AAGTATCTGG TGACACATTG 
GGTATTAAAA GCAGTCCTGT GTCTAAAGCC CATGCGGAGG CACAGGGGAC TCCTGAAAAA 
AATTCACAGA GTCCTAATGA TTCTCTAGCT AGTCAAGAAC CAAGAAGGTC TGGCAGAAGG 
CGAAGTAAAC CTGTTCGTCC AGGGCAAGAT GCAGAGGAAA TAAAAAGTAA ATCTCAAGAA 
CAAGCCAGTC AACCTGCAGA CTCTGCGATA ATAAACTCAC AAAAGCCCCT TCTTGCTTCA 
CCTAGTAGTA CTTTGACTGG AAGAAGGAAA AAAAAGGTTG AGACCAATAA AATGGAAGAC 
CAGCTGAACA ATGAAGAAAT GAAAGGAGAT CTCAGCCAAA GCGATTCACA GATTTCCGCT 
GCTGACCCTC CACAGACAGT AGGTGTGCAT GCCAATAAAG ACTTAAGCCA GACTGAGTTA 
TCTCCGAAGA GTTCTCCTGT AACCCAGTCA AATAGCCAAA GCAAAGAGAG ACTGCCAGCT 
CTCTGCGAGT CTGAGAGTAT GTCGTTAGGT AGACAGGAGA CAAAAACACA ACTTGAAGAA 
TCCACAGAAA AGGAGACAAA TAATGACTCT CAAAACGCTT CATCTGCAGC GAGACCGAAG 
CATATACAGT CTACCACGTC TGACTCCGAG TCGGATAGCC AGCAAATTGT CCGTAAACGA 
AGATCGAGGA GATCTGAAAC TAAGTTACTG GAGATATCTG GTGTGCCGAT CAAAGAAGAC 
TTGAATCAGA CAGACTCCCA AACTCCAACA GGAGTGAAGG GAAGAGCTAT GAGGAGGAGA 
ACCGTAGAAG GTGCAGAGTC TAGTAAAGAT TCCACATCAG CGGTAAATAC AGAGGAGTTA 
AGTGCAGGCA AGGAGTCATC ACAAGAGCTT GGTAGATACA GAACTCGACG TTCTAGGGGT 
TTGTTGTCTC CTGAGAGTAT GGAGTCTGAA AACTCTGATA GCCGAGAAGA TGGGGCAAGG 
CCTAAAAAGA GGCTGCGAAA ACCACCATCA ACAGAGGTGT TACCTGTTGC TTCAGAACCC 
AAAGTTATTG AGATGGTACC AGAAAAGGAC GATGTTCAGT CAGAGGTTTT AATGGAGACG 
TCAAAGGATG AGGCAGATCC TGAAAATATC TCGAATACTT CAGTCAGTGA AACCAAAGAA 
CTTGAGCAAA AGAATAAAGA GGAAGTCCCT GCCCCTTGTG ATGCACCACA TGAACAGATT 
ATCACGGAAA AAGCAGCATC AAACAACAAA ATTGACACTG ACTTAACTGA TACAGACGAA 
AAAGAAACCG TCAAGGCAGA GGGTGAAGAA GCTAATGAAT CTTCGCAAAC TGGATCAGAA 
GATTTTAAAA AGACAGATGT TTTGCATAAA TGCCCACATA CAAAGGGACG GGGCCGTGCA 
CGTAGACGAT CTAAAAACTG CAACTGCTTT TCCAACAACC GTGATGTGTT TTCACAGGAC 
AGTGACTTCC AAGAATCACA AGATCTAAAA AATGAACAGG TTCCACTTGC TGCCAACATC 
TTGAACTCTC AACCTGATCA GTCAAGCCCA TCAACTGCAT CAGAAGAAAA CAAAAGCTCA 
GCAATTGAAG AAGTCCCTGT TGCGGATGTT CCATCACTTT CAGCCTTATC AGCTGATCTC 
CCATCTGAGG TTTCACCTTC TGAAAGTCCC ATTACAAAAC TTGGGGTAAT TGTTTCTCAG 
CTACCAGAAG TGGAGAAAGA AGCACAGCCT GGATCATCCA AACCAATGAA GGATTCGAGC 
AAGGAGCTTG TAGAAGAAGT GAATCGATTA AGTTCTGGAT CCACAGGTGG TCAAAAGCAC 
AAAGGAGAAA ATAGATTAGA AAACACTTCA GTAAGCCAAG AACCTGTCCA GAGTTCAACA 
CCTCAAGACA CGTCTCCAAG TCAAGGTCGT CCAGTGGACG ATGCACCAAT ATGCCAGGAG 
CAGTTAGAGT CTTCTCATGT CCAGCAGGAA GATGCAGTTA ACCCAGCAGT GATGGAAAGA 
GAAGACCTTG CTGTGTCAGA GCAGAGACAA GAGAAGGCTG TGTTGTCTGA AGAGTTAGAT 
GGAAAAGGTT TGCTTGACAA GGAAACTACT CTTGTTGAAA AGGAAGAAGA GATGCAGGTT 
AATAACCAGG ATCTTCCTGT GGATGTCCAA GAAGAGTTAC CTGTAGTGTC CCAGAGTAGC 
ACATCTGAAG CCTGCTTGGA TTCTCCACCC AAGCCAAAAC TGTTAAGCGC ACTCAGTGGT 
GAACTAGAGC CTGGCCAGAG TCCAACTAGA AGTCGGTCTC GAGTCTGGTC ACCATCTGCT 
TCTCCGTCTA CCAGTATTCT GAAGAAGGGT CAGAAGAGAG CATGTGAAGA GGAGACTCCA 
TCCCCCATTC CCAAGTCACG TCGAGTGTCT TTTGCAACTC CAATTTACCA GCAAGAACTG 
GCTGATGACA TTGATCGTCG TAGTCCGGTT ATTCGCACAA GTTCACCTAG GTCAAAAGTC 
TTGAGTGGAC AGTCAAAGTA TATCACTACA CCAACAAAAG GTGGCTATAC AAGTCTGAGT 
CCACGTAACC TCCGTAGCCC TGGATCCAAA AGCTCTAAAA AATGTCTGAT TTCAGAGATG 
AGCCAGGAGC CACGTCCTAT TCCTAAAGAT TGTGTTTATC CAGCACTTGT TGGCTGCTCT 
ACTCCAGTAG AAGCTGTTCT GCCACAGATT TCCTCCAACA TGTGGCCGCG TGGCTTCGGC 
CAGCTTGTTC GAGCCAGGAA CATTAAGACA GTAGGAGATC TGAGTGCTCT TACCCCTAAT 
GAAATCAAGT CTCTTCCTAT TCGCTCACCT AAGCTGTCCA ATGTGAGGAA AGCACTTAAA 
ACATATCATG AACAACAGAG AAAAGGACGT TCGGATGACC TGAAGAGTTT TGACGAAATG 
GAGAAAATGA CGTCTGAACC TGAAGAGATG GAGCTTCCTC AAAACCAAGA TGAGGAGCAA 
ACATCAGGAC CAGAGGCTCA AACAGCAGAA TCAGAGACTC AAGAAGACAA AGCCTCAGAA 
GTGGAAGTAA ATTCAGAAGT GGTGACTGAA GAGAACCAAT CTCATGATTT GCTCTCAGAT 
ATTGTAGCTC TTGGTGAACG CTTTACGCCT GAGGAACTCA GTCAGCATTC AGCCAGTCAG 
TTGGGTCTGA TGCATGAACA TCTGAGTGGA ATGATGAGGA GCATTGTGAC CAATCTGCAG 
TCCCGCTTGC TCAGTAATCT GGACGATAGC TTGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_005167575.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167632.1 Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X1, mRNA.

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CloneID	ODa15091
Clone ID Related Accession (Same CDS sequence)	XM_005167575.3 , XM_005167575.4 , XM_009304632.2
Accession Version	XM_005167575.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	telomere-associated protein RIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167575.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CAGTGCCTTT ATCCAGTACC AGCCTCCTTC CTCTTCTGGA GTGTTTGGAA 
GATGAATCAG CTGGGCTTTC AGCGCAGACG GATGCCTACA TCACCATTGC AAACCGACTT 
AATGGAGAAG AAGGACGCCA GTTTCTGCCT GTGGTTGTTA AGCATTTTGC ACGTCTTAGT 
AAAGTGCTTT TGGTTCACAT CTCCAGTGAG AATGAGGAGC TGTGTCAGGC TGCACTGCAG 
GCTTTGGGAT TCTGTGTCTT CCACTCACAC ATAGTTTCAG TCATACCAGC AAATATTTCA 
GAGAATATAT TGTCTACGCT CTGCAATGTT GTGGTAAAGT CCAAAGAGAA GTTTACGTGC 
ACTCGGGCAT TGTGGGTCAT TTCAAAACAG AATTTCCCTC CAGAGGTGGC ATCCAAAATG 
GCCCCAGAGA TTCTCAAGAG CCTAGAAGCG ATGCAGACCA GAGAGGTGCA GTCCATCCTC 
ATTGACCACG AGTCACTCAA TGTCATCATA AGGTTACTGG AGCAGGCCCC TGTTCCGATG 
GCTGTTGGTG CTGTTTGCTG GGCTAAACTT GTTATTCCTC TGGTGGTTCA TTCGGCCAAT 
AAAGTGCGTC TGCGAGCGGC AGCTGCCCTG GAGCTCGGCA TGCCTCTTCT TCTGGAGAAA 
CAGCAGGAAG TGGCAGCCAT TGTTGAGCCA ATGATGTCTT CTGTGCTCAT CCCTGAAATG 
CAGAAGCTCT TTGCGTCTAA GAACGAGACA AACATGTTGA AACTCTGGCC TTTGTTTGTA 
AGACTTCTGG GCAAGTTACT CCACAAAGGC GGTGCTTTCA TTAACTCCTT GCTGTATCTA 
GAGGAGCTGG GTTTCCGCAA CTCTTCTCCT AATATTAAGA AGATTGCTTT CATAGCTTGG 
AAGAGCCTCA TTGACAATTT TGCCCTTAAT CCAGATATCC TGTGCAGTAG CAAGCGTCTG 
AAGCTCCTCA TGCAGCCGCT CAGCTCCATC CAAGTCAGGA CTGAAGCTCT TCTGCTTACC 
AAGCTGGAGG TCTGGTGGTA TCTTGCTGTC AAGCTCGGAC CCAACCTGTC TGCTAACTTT 
GAACAGGTTG GCGTGCCTCT ATTGCACAGC ACACTTCCTT CTGATTCTCC ACTTCAGAGC 
CCGACTACAC CTGCTCGAAC CTCTAACCCA AGCAACGGCA CACCTAACAC CCCTAAGTCA 
GGCATTCCCT CCTGCAGCAC GCCGAGCACG ACTCCTCGCA TGAATCTGAA CAGCAGCATG 
CAGGCGCCTC AGTCCTATCG TTCCATTCAG CTCCTGGGAC TAGAGATGCT CCTGCACTGG 
CTTGTGGGTC CAGAGGTCAC AGTCACAGCT GCTCGAGAAA ACCTTCAGCT CAGCCTGGAG 
CCCCTGACGC ACCCGCTTTT TACCAGCCCT TCCACCTTCA GCAGACATGC TTCTACCCTC 
ATATCTGCCA GCAAAGAAGG CATCATTACT GTAGGGAAGC ATGCTCCAGA AGCACTTCTT 
AATCTTATTT GGAGCAACCT GATCATTTCT GTCAGTGCAG CAATTGAAGC TGGAAATAAA 
AAGGAGCGAC AGGGTTCAGA GGTCCTCACA CTTTTCCTGC AGGCATTGCA GTCCATTTTG 
TCATCAGACG CTCTGCCTCC TGAACGTGCA CTGCTTCTGT TGGAAGCCAC TGTAAAGGGA 
ATACCTCAGA AGGTGCTTGG TTCTGCAGCT TATCAAGTGG CAAACATGGA CGTGTTAAAC 
GGTACTCCTG CACTTTTCCT AATACTGCTG TTCTATAAAA GTAACCAGCT CTCATCATTT 
CTTGAAGACG AAAGATTTTT TTCAAGCTTG GAGACCCTTG TAAGCTGTGG GTTTTGTGGA 
CCCACATCGC CCTTGGCATT TGGAGAGGCG GTTTTAGGAG CAGTTAATGG GAGTGTGGAG 
ACTGTTAAAA ACAAGAAGCA GCTCTGGAGA ATGTGGAGTA TGGTTGTAAG TCCTCTGACG 
GACACAATTA CTCAGACCAA TGAAGTCAAC CAAGGCGATG CTCTGGAGCA TAACTTCAGT 
GCCATTTACA GTGCGTTGAT GTTCCCCGTC ATCCACCTGC TGCCTTATTC AGCCTTACCA 
CAGACCACTC AGAAGGCCAT GATCAGCACA TGGTCCAGGC TTTACAAGGC TTTTGCCCGT 
TGCTCAGCTT TGGTTGCCAC AGCAGAGGAG AATACATGCT GTGAAGAGTT GTGTGTCAAG 
ATCTCTGCCT CTCTTGACCG TGAAGCTCTA AAAAGTCTGT CTATGCTAGA TGCAATAGCA 
CACATCTTGC TCATCATCGT TGAGAGTATG GACTTTTCTC CATATACCCC TCAGTTCCAG 
CAGAAGATGA AATCTCCTCG TACACCCATG AGTTGGGTAC GCAAGAGAAG CAGAGCCCTC 
GGGAATCTCT CAACCTTCCA CACTCTTCTG ATACAGACTC TAGAGGCTTT TCTCACTGCA 
GACCATTCAG AAGAATCTGC AGAGCCTTTA AATGGAATTG GCTCTGCCTT GGTGTCAATC 
TTGACCTCTC TTTTCACTAA TATTACCCTT CCCTCATTCT TACAAGAGGT GCTCTCCAGC 
CTCACCAAAC CACTTGCACG TCTTTATGAG CAGTTCTCCT GTTGTGATGA CCCATCAAAG 
CTCAGTCCAG AACTAGGGCA CAAGCTGGAG AAACTGGCCG CAGACTTGCT AGGCTGCCTT 
CAGACACATT TGACTCTCCA TGATGATGAA TTCCTCACTG TGTTGTCGCC CTTGCTTTGT 
GTACTTTTTT TGCACAAGAG CAAACAGGTT TGCTCTGTGG TCACTCAATT CTGGAATGCC 
ACTTTTGGAA ATGCGCTCAC CTTGAGTTAC CCAGAACCTC TGAAGTGTGT TTTAAATCAG 
GCTAAGCAGA AGACTCCACT GATTTTACCT GGTTTTGAAG TTGTTGATGT ACCAGATGAT 
TACAGTGGAA CATATATGAG TGAGAGTTCT CAGCTGGAGC CTCAGATTAG CGGGGTAAAA 
GTGACCTCTG TGGGAAAGAG GGACTCTCTT TTGGCAAGAG CAGAAGAGCT AACAGGAAAA 
AGTTCTGTAA CCACATCAAA ACCAGTGTCT GTAAAGCTGG ACTTTGGCTC CCCCAAGCCC 
TTAAGACGAG AAGCTCTTGA GGAGGAAGCA TCTGAAGATT TTGTTTTTAT TCCACCTGAG 
ATGAAAGAGA GAGTCCTGAC TGAGCATCAG AAGGAAGTGA AAAGGACTAA ACGGGTTGAC 
ATTCCTGCCC TGTACAATAA TCTTGATGCC TCCATGGACA CCACAGTCTT TTCACAGTAT 
ACTCAGAGTC AGGAGGAGTC AATGGACACA TTGCCTAAAG GTGAGAAGGA TCATCCTGTG 
GAAGCGGCTA GTGTTCAACT GAGGGAGGTT GCCTCAAATG AAGTACCAAC CACAGATGTT 
AAAGTCCAAA AAGTCACCAG TTCTGATATA GAAACTCCAG ATAAAGCTAC GCCGAATAAA 
GATCAAACTT TGGAGATGGA TCAATCACCA TCTACTGGTG TCTCAGTTGA TGTGAGTGGG 
CTTGAGAAAA GCGCATTGGA GGGGACAAGT CCTAATATTT CAAGTTCATC TGATGTGGTA 
TCTGGCACAC CTCCTAAACC TAACAGCAGG CGTCAGTCAT TTATAACCTT AGAAAAATAT 
GCTGAAGGGA AATCGACAAG TCCTGCAAGT CCTGCATTTA CAGGTACGCT CACCCGGAGA 
TCAAGAAGAC TGGAGGCTTC ACAGGCATCA ACATCAAATC AAAATGATTC TCAACCTCAG 
ATTAAAAAAG TGGATTCTCA GGAATCTCAA AAGATGAGCA GCATTGACAT GTCCATTCAA 
AATGAGCATG CTAGCATGGA AGTAAAAGAT GAGAAGTCAG TGGGAGCTAA TCCATGTGAA 
GGTACAGATG AGGAAGATGT TGTTCCAGAT ACCCAAACCC AAGTATCTGG TGACACATTG 
GGTATTAAAA GCAGTCCTGT GTCTAAAGCC CATGCGGAGG CACAGGGGAC TCCTGAAAAA 
AATTCACAGA GTCCTAATGA TTCTCTAGCT AGTCAAGAAC CAAGAAGGTC TGGCAGAAGG 
CGAAGTAAAC CTGTTCGTCC AGGGCAAGAT GCAGAGGAAA TAAAAAGTAA ATCTCAAGAA 
CAAGCCAGTC AACCTGCAGA CTCTGCGATA ATAAACTCAC AAAAGCCCCT TCTTGCTTCA 
CCTAGTAGTA CTTTGACTGG AAGAAGGAAA AAAAAGGTTG AGACCAATAA AATGGAAGAC 
CAGCTGAACA ATGAAGAAAT GAAAGGAGAT CTCAGCCAAA GCGATTCACA GATTTCCGCT 
GCTGACCCTC CACAGACAGT AGGTGTGCAT GCCAATAAAG ACTTAAGCCA GACTGAGTTA 
TCTCCGAAGA GTTCTCCTGT AACCCAGTCA AATAGCCAAA GCAAAGAGAG ACTGCCAGCT 
CTCTGCGAGT CTGAGAGTAT GTCGTTAGGT AGACAGGAGA CAAAAACACA ACTTGAAGAA 
TCCACAGAAA AGGAGACAAA TAATGACTCT CAAAACGCTT CATCTGCAGC GAGACCGAAG 
CATATACAGT CTACCACGTC TGACTCCGAG TCGGATAGCC AGCAAATTGT CCGTAAACGA 
AGATCGAGGA GATCTGAAAC TAAGTTACTG GAGATATCTG GTGTGCCGAT CAAAGAAGAC 
TTGAATCAGA CAGACTCCCA AACTCCAACA GGAGTGAAGG GAAGAGCTAT GAGGAGGAGA 
ACCGTAGAAG GTGCAGAGTC TAGTAAAGAT TCCACATCAG CGGTAAATAC AGAGGAGTTA 
AGTGCAGGCA AGGAGTCATC ACAAGAGCTT GGTAGATACA GAACTCGACG TTCTAGGGGT 
TTGTTGTCTC CTGAGAGTAT GGAGTCTGAA AACTCTGATA GCCGAGAAGA TGGGGCAAGG 
CCTAAAAAGA GGCTGCGAAA ACCACCATCA ACAGAGGTGT TACCTGTTGC TTCAGAACCC 
AAAGTTATTG AGATGGTACC AGAAAAGGAC GATGTTCAGT CAGAGGTTTT AATGGAGACG 
TCAAAGGATG AGGCAGATCC TGAAAATATC TCGAATACTT CAGTCAGTGA AACCAAAGAA 
CTTGAGCAAA AGAATAAAGA GGAAGTCCCT GCCCCTTGTG ATGCACCACA TGAACAGATT 
ATCACGGAAA AAGCAGCATC AAACAACAAA ATTGACACTG ACTTAACTGA TACAGACGAA 
AAAGAAACCG TCAAGGCAGA GGGTGAAGAA GCTAATGAAT CTTCGCAAAC TGGATCAGAA 
GATTTTAAAA AGACAGATGT TTTGCATAAA TGCCCACATA CAAAGGGACG GGGCCGTGCA 
CGTAGACGAT CTAAAAACTG CAACTGCTTT TCCAACAACC GTGATGTGTT TTCACAGGAC 
AGTGACTTCC AAGAATCACA AGATCTAAAA AATGAACAGG TTCCACTTGC TGCCAACATC 
TTGAACTCTC AACCTGATCA GTCAAGCCCA TCAACTGCAT CAGAAGAAAA CAAAAGCTCA 
GCAATTGAAG AAGTCCCTGT TGCGGATGTT CCATCACTTT CAGCCTTATC AGCTGATCTC 
CCATCTGAGG TTTCACCTTC TGAAAGTCCC ATTACAAAAC TTGGGGTAAT TGTTTCTCAG 
CTACCAGAAG TGGAGAAAGA AGCACAGCCT GGATCATCCA AACCAATGAA GGATTCGAGC 
AAGGAGCTTG TAGAAGAAGT GAATCGATTA AGTTCTGGAT CCACAGGTGG TCAAAAGCAC 
AAAGGAGAAA ATAGATTAGA AAACACTTCA GTAAGCCAAG AACCTGTCCA GAGTTCAACA 
CCTCAAGACA CGTCTCCAAG TCAAGGTCGT CCAGTGGACG ATGCACCAAT ATGCCAGGAG 
CAGTTAGAGT CTTCTCATGT CCAGCAGGAA GATGCAGTTA ACCCAGCAGT GATGGAAAGA 
GAAGACCTTG CTGTGTCAGA GCAGAGACAA GAGAAGGCTG TGTTGTCTGA AGAGTTAGAT 
GGAAAAGGTT TGCTTGACAA GGAAACTACT CTTGTTGAAA AGGAAGAAGA GATGCAGGTT 
AATAACCAGG ATCTTCCTGT GGATGTCCAA GAAGAGTTAC CTGTAGTGTC CCAGAGTAGC 
ACATCTGAAG CCTGCTTGGA TTCTCCACCC AAGCCAAAAC TGTTAAGCGC ACTCAGTGGT 
GAACTAGAGC CTGGCCAGAG TCCAACTAGA AGTCGGTCTC GAGTCTGGTC ACCATCTGCT 
TCTCCGTCTA CCAGTATTCT GAAGAAGGGT CAGAAGAGAG CATGTGAAGA GGAGACTCCA 
TCCCCCATTC CCAAGTCACG TCGAGTGTCT TTTGCAACTC CAATTTACCA GCAAGAACTG 
GCTGATGACA TTGATCGTCG TAGTCCGGTT ATTCGCACAA GTTCACCTAG GTCAAAAGTC 
TTGAGTGGAC AGTCAAAGTA TATCACTACA CCAACAAAAG GTGGCTATAC AAGTCTGAGT 
CCACGTAACC TCCGTAGCCC TGGATCCAAA AGCTCTAAAA AATGTCTGAT TTCAGAGATG 
AGCCAGGAGC CACGTCCTAT TCCTAAAGAT TGTGTTTATC CAGCACTTGT TGGCTGCTCT 
ACTCCAGTAG AAGCTGTTCT GCCACAGATT TCCTCCAACA TGTGGCCGCG TGGCTTCGGC 
CAGCTTGTTC GAGCCAGGAA CATTAAGACA GTAGGAGATC TGAGTGCTCT TACCCCTAAT 
GAAATCAAGT CTCTTCCTAT TCGCTCACCT AAGCTGTCCA ATGTGAGGAA AGCACTTAAA 
ACATATCATG AACAACAGAG AAAAGGACGT TCGGATGACC TGAAGAGTTT TGACGAAATG 
GAGAAAATGA CGTCTGAACC TGAAGAGATG GAGCTTCCTC AAAACCAAGA TGAGGAGCAA 
ACATCAGGAC CAGAGGCTCA AACAGCAGAA TCAGAGACTC AAGAAGACAA AGCCTCAGAA 
GTGGAAGTAA ATTCAGAAGT GGTGACTGAA GAGAACCAAT CTCATGATTT GCTCTCAGAT 
ATTGTAGCTC TTGGTGAACG CTTTACGCCT GAGGAACTCA GTCAGCATTC AGCCAGTCAG 
TTGGGTCTGA TGCATGAACA TCTGAGTGGA ATGATGAGGA GCATTGTGAC CAATCTGCAG 
TCCCGCTTGC TCAGTAATCT GGACGATAGC TTGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167632.1
CDS	192..7370
Translation	MMAAVPLSSTSLLPLLECLEDESAGLSAQTDAYITIANRLNGEEGRQFLPVVVKHFARLSKVLLVHISSENEELCQAALQALGFCVFHSHIVSVIPANISENILSTLCNVVVKSKEKFTCTRALWVISKQNFPPEVASKMAPEILKSLEAMQTREVQSILIDHESLNVIIRLLEQAPVPMAVGAVCWAKLVIPLVVHSANKVRLRAAAALELGMPLLLEKQQEVAAIVEPMMSSVLIPEMQKLFASKNETNMLKLWPLFVRLLGKLLHKGGAFINSLLYLEELGFRNSSPNIKKIAFIAWKSLIDNFALNPDILCSSKRLKLLMQPLSSIQVRTEALLLTKLEVWWYLAVKLGPNLSANFEQVGVPLLHSTLPSDSPLQSPTTPARTSNPSNGTPNTPKSGIPSCSTPSTTPRMNLNSSMQAPQSYRSIQLLGLEMLLHWLVGPEVTVTAARENLQLSLEPLTHPLFTSPSTFSRHASTLISASKEGIITVGKHAPEALLNLIWSNLIISVSAAIEAGNKKERQGSEVLTLFLQALQSILSSDALPPERALLLLEATVKGIPQKVLGSAAYQVANMDVLNGTPALFLILLFYKSNQLSSFLEDERFFSSLETLVSCGFCGPTSPLAFGEAVLGAVNGSVETVKNKKQLWRMWSMVVSPLTDTITQTNEVNQGDALEHNFSAIYSALMFPVIHLLPYSALPQTTQKAMISTWSRLYKAFARCSALVATAEENTCCEELCVKISASLDREALKSLSMLDAIAHILLIIVESMDFSPYTPQFQQKMKSPRTPMSWVRKRSRALGNLSTFHTLLIQTLEAFLTADHSEESAEPLNGIGSALVSILTSLFTNITLPSFLQEVLSSLTKPLARLYEQFSCCDDPSKLSPELGHKLEKLAADLLGCLQTHLTLHDDEFLTVLSPLLCVLFLHKSKQVCSVVTQFWNATFGNALTLSYPEPLKCVLNQAKQKTPLILPGFEVVDVPDDYSGTYMSESSQLEPQISGVKVTSVGKRDSLLARAEELTGKSSVTTSKPVSVKLDFGSPKPLRREALEEEASEDFVFIPPEMKERVLTEHQKEVKRTKRVDIPALYNNLDASMDTTVFSQYTQSQEESMDTLPKGEKDHPVEAASVQLREVASNEVPTTDVKVQKVTSSDIETPDKATPNKDQTLEMDQSPSTGVSVDVSGLEKSALEGTSPNISSSSDVVSGTPPKPNSRRQSFITLEKYAEGKSTSPASPAFTGTLTRRSRRLEASQASTSNQNDSQPQIKKVDSQESQKMSSIDMSIQNEHASMEVKDEKSVGANPCEGTDEEDVVPDTQTQVSGDTLGIKSSPVSKAHAEAQGTPEKNSQSPNDSLASQEPRRSGRRRSKPVRPGQDAEEIKSKSQEQASQPADSAIINSQKPLLASPSSTLTGRRKKKVETNKMEDQLNNEEMKGDLSQSDSQISAADPPQTVGVHANKDLSQTELSPKSSPVTQSNSQSKERLPALCESESMSLGRQETKTQLEESTEKETNNDSQNASSAARPKHIQSTTSDSESDSQQIVRKRRSRRSETKLLEISGVPIKEDLNQTDSQTPTGVKGRAMRRRTVEGAESSKDSTSAVNTEELSAGKESSQELGRYRTRRSRGLLSPESMESENSDSREDGARPKKRLRKPPSTEVLPVASEPKVIEMVPEKDDVQSEVLMETSKDEADPENISNTSVSETKELEQKNKEEVPAPCDAPHEQIITEKAASNNKIDTDLTDTDEKETVKAEGEEANESSQTGSEDFKKTDVLHKCPHTKGRGRARRRSKNCNCFSNNRDVFSQDSDFQESQDLKNEQVPLAANILNSQPDQSSPSTASEENKSSAIEEVPVADVPSLSALSADLPSEVSPSESPITKLGVIVSQLPEVEKEAQPGSSKPMKDSSKELVEEVNRLSSGSTGGQKHKGENRLENTSVSQEPVQSSTPQDTSPSQGRPVDDAPICQEQLESSHVQQEDAVNPAVMEREDLAVSEQRQEKAVLSEELDGKGLLDKETTLVEKEEEMQVNNQDLPVDVQEELPVVSQSSTSEACLDSPPKPKLLSALSGELEPGQSPTRSRSRVWSPSASPSTSILKKGQKRACEEETPSPIPKSRRVSFATPIYQQELADDIDRRSPVIRTSSPRSKVLSGQSKYITTPTKGGYTSLSPRNLRSPGSKSSKKCLISEMSQEPRPIPKDCVYPALVGCSTPVEAVLPQISSNMWPRGFGQLVRARNIKTVGDLSALTPNEIKSLPIRSPKLSNVRKALKTYHEQQRKGRSDDLKSFDEMEKMTSEPEEMELPQNQDEEQTSGPEAQTAESETQEDKASEVEVNSEVVTEENQSHDLLSDIVALGERFTPEELSQHSASQLGLMHEHLSGMMRSIVTNLQSRLLSNLDDSLP

Target ORF information:

RefSeq Version	XM_005167575.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167575.4

ORF Insert Sequence:

ATGATGGCGG CAGTGCCTTT ATCCAGTACC AGCCTCCTTC CTCTTCTGGA GTGTTTGGAA 
GATGAATCAG CTGGGCTTTC AGCGCAGACG GATGCCTACA TCACCATTGC AAACCGACTT 
AATGGAGAAG AAGGACGCCA GTTTCTGCCT GTGGTTGTTA AGCATTTTGC ACGTCTTAGT 
AAAGTGCTTT TGGTTCACAT CTCCAGTGAG AATGAGGAGC TGTGTCAGGC TGCACTGCAG 
GCTTTGGGAT TCTGTGTCTT CCACTCACAC ATAGTTTCAG TCATACCAGC AAATATTTCA 
GAGAATATAT TGTCTACGCT CTGCAATGTT GTGGTAAAGT CCAAAGAGAA GTTTACGTGC 
ACTCGGGCAT TGTGGGTCAT TTCAAAACAG AATTTCCCTC CAGAGGTGGC ATCCAAAATG 
GCCCCAGAGA TTCTCAAGAG CCTAGAAGCG ATGCAGACCA GAGAGGTGCA GTCCATCCTC 
ATTGACCACG AGTCACTCAA TGTCATCATA AGGTTACTGG AGCAGGCCCC TGTTCCGATG 
GCTGTTGGTG CTGTTTGCTG GGCTAAACTT GTTATTCCTC TGGTGGTTCA TTCGGCCAAT 
AAAGTGCGTC TGCGAGCGGC AGCTGCCCTG GAGCTCGGCA TGCCTCTTCT TCTGGAGAAA 
CAGCAGGAAG TGGCAGCCAT TGTTGAGCCA ATGATGTCTT CTGTGCTCAT CCCTGAAATG 
CAGAAGCTCT TTGCGTCTAA GAACGAGACA AACATGTTGA AACTCTGGCC TTTGTTTGTA 
AGACTTCTGG GCAAGTTACT CCACAAAGGC GGTGCTTTCA TTAACTCCTT GCTGTATCTA 
GAGGAGCTGG GTTTCCGCAA CTCTTCTCCT AATATTAAGA AGATTGCTTT CATAGCTTGG 
AAGAGCCTCA TTGACAATTT TGCCCTTAAT CCAGATATCC TGTGCAGTAG CAAGCGTCTG 
AAGCTCCTCA TGCAGCCGCT CAGCTCCATC CAAGTCAGGA CTGAAGCTCT TCTGCTTACC 
AAGCTGGAGG TCTGGTGGTA TCTTGCTGTC AAGCTCGGAC CCAACCTGTC TGCTAACTTT 
GAACAGGTTG GCGTGCCTCT ATTGCACAGC ACACTTCCTT CTGATTCTCC ACTTCAGAGC 
CCGACTACAC CTGCTCGAAC CTCTAACCCA AGCAACGGCA CACCTAACAC CCCTAAGTCA 
GGCATTCCCT CCTGCAGCAC GCCGAGCACG ACTCCTCGCA TGAATCTGAA CAGCAGCATG 
CAGGCGCCTC AGTCCTATCG TTCCATTCAG CTCCTGGGAC TAGAGATGCT CCTGCACTGG 
CTTGTGGGTC CAGAGGTCAC AGTCACAGCT GCTCGAGAAA ACCTTCAGCT CAGCCTGGAG 
CCCCTGACGC ACCCGCTTTT TACCAGCCCT TCCACCTTCA GCAGACATGC TTCTACCCTC 
ATATCTGCCA GCAAAGAAGG CATCATTACT GTAGGGAAGC ATGCTCCAGA AGCACTTCTT 
AATCTTATTT GGAGCAACCT GATCATTTCT GTCAGTGCAG CAATTGAAGC TGGAAATAAA 
AAGGAGCGAC AGGGTTCAGA GGTCCTCACA CTTTTCCTGC AGGCATTGCA GTCCATTTTG 
TCATCAGACG CTCTGCCTCC TGAACGTGCA CTGCTTCTGT TGGAAGCCAC TGTAAAGGGA 
ATACCTCAGA AGGTGCTTGG TTCTGCAGCT TATCAAGTGG CAAACATGGA CGTGTTAAAC 
GGTACTCCTG CACTTTTCCT AATACTGCTG TTCTATAAAA GTAACCAGCT CTCATCATTT 
CTTGAAGACG AAAGATTTTT TTCAAGCTTG GAGACCCTTG TAAGCTGTGG GTTTTGTGGA 
CCCACATCGC CCTTGGCATT TGGAGAGGCG GTTTTAGGAG CAGTTAATGG GAGTGTGGAG 
ACTGTTAAAA ACAAGAAGCA GCTCTGGAGA ATGTGGAGTA TGGTTGTAAG TCCTCTGACG 
GACACAATTA CTCAGACCAA TGAAGTCAAC CAAGGCGATG CTCTGGAGCA TAACTTCAGT 
GCCATTTACA GTGCGTTGAT GTTCCCCGTC ATCCACCTGC TGCCTTATTC AGCCTTACCA 
CAGACCACTC AGAAGGCCAT GATCAGCACA TGGTCCAGGC TTTACAAGGC TTTTGCCCGT 
TGCTCAGCTT TGGTTGCCAC AGCAGAGGAG AATACATGCT GTGAAGAGTT GTGTGTCAAG 
ATCTCTGCCT CTCTTGACCG TGAAGCTCTA AAAAGTCTGT CTATGCTAGA TGCAATAGCA 
CACATCTTGC TCATCATCGT TGAGAGTATG GACTTTTCTC CATATACCCC TCAGTTCCAG 
CAGAAGATGA AATCTCCTCG TACACCCATG AGTTGGGTAC GCAAGAGAAG CAGAGCCCTC 
GGGAATCTCT CAACCTTCCA CACTCTTCTG ATACAGACTC TAGAGGCTTT TCTCACTGCA 
GACCATTCAG AAGAATCTGC AGAGCCTTTA AATGGAATTG GCTCTGCCTT GGTGTCAATC 
TTGACCTCTC TTTTCACTAA TATTACCCTT CCCTCATTCT TACAAGAGGT GCTCTCCAGC 
CTCACCAAAC CACTTGCACG TCTTTATGAG CAGTTCTCCT GTTGTGATGA CCCATCAAAG 
CTCAGTCCAG AACTAGGGCA CAAGCTGGAG AAACTGGCCG CAGACTTGCT AGGCTGCCTT 
CAGACACATT TGACTCTCCA TGATGATGAA TTCCTCACTG TGTTGTCGCC CTTGCTTTGT 
GTACTTTTTT TGCACAAGAG CAAACAGGTT TGCTCTGTGG TCACTCAATT CTGGAATGCC 
ACTTTTGGAA ATGCGCTCAC CTTGAGTTAC CCAGAACCTC TGAAGTGTGT TTTAAATCAG 
GCTAAGCAGA AGACTCCACT GATTTTACCT GGTTTTGAAG TTGTTGATGT ACCAGATGAT 
TACAGTGGAA CATATATGAG TGAGAGTTCT CAGCTGGAGC CTCAGATTAG CGGGGTAAAA 
GTGACCTCTG TGGGAAAGAG GGACTCTCTT TTGGCAAGAG CAGAAGAGCT AACAGGAAAA 
AGTTCTGTAA CCACATCAAA ACCAGTGTCT GTAAAGCTGG ACTTTGGCTC CCCCAAGCCC 
TTAAGACGAG AAGCTCTTGA GGAGGAAGCA TCTGAAGATT TTGTTTTTAT TCCACCTGAG 
ATGAAAGAGA GAGTCCTGAC TGAGCATCAG AAGGAAGTGA AAAGGACTAA ACGGGTTGAC 
ATTCCTGCCC TGTACAATAA TCTTGATGCC TCCATGGACA CCACAGTCTT TTCACAGTAT 
ACTCAGAGTC AGGAGGAGTC AATGGACACA TTGCCTAAAG GTGAGAAGGA TCATCCTGTG 
GAAGCGGCTA GTGTTCAACT GAGGGAGGTT GCCTCAAATG AAGTACCAAC CACAGATGTT 
AAAGTCCAAA AAGTCACCAG TTCTGATATA GAAACTCCAG ATAAAGCTAC GCCGAATAAA 
GATCAAACTT TGGAGATGGA TCAATCACCA TCTACTGGTG TCTCAGTTGA TGTGAGTGGG 
CTTGAGAAAA GCGCATTGGA GGGGACAAGT CCTAATATTT CAAGTTCATC TGATGTGGTA 
TCTGGCACAC CTCCTAAACC TAACAGCAGG CGTCAGTCAT TTATAACCTT AGAAAAATAT 
GCTGAAGGGA AATCGACAAG TCCTGCAAGT CCTGCATTTA CAGGTACGCT CACCCGGAGA 
TCAAGAAGAC TGGAGGCTTC ACAGGCATCA ACATCAAATC AAAATGATTC TCAACCTCAG 
ATTAAAAAAG TGGATTCTCA GGAATCTCAA AAGATGAGCA GCATTGACAT GTCCATTCAA 
AATGAGCATG CTAGCATGGA AGTAAAAGAT GAGAAGTCAG TGGGAGCTAA TCCATGTGAA 
GGTACAGATG AGGAAGATGT TGTTCCAGAT ACCCAAACCC AAGTATCTGG TGACACATTG 
GGTATTAAAA GCAGTCCTGT GTCTAAAGCC CATGCGGAGG CACAGGGGAC TCCTGAAAAA 
AATTCACAGA GTCCTAATGA TTCTCTAGCT AGTCAAGAAC CAAGAAGGTC TGGCAGAAGG 
CGAAGTAAAC CTGTTCGTCC AGGGCAAGAT GCAGAGGAAA TAAAAAGTAA ATCTCAAGAA 
CAAGCCAGTC AACCTGCAGA CTCTGCGATA ATAAACTCAC AAAAGCCCCT TCTTGCTTCA 
CCTAGTAGTA CTTTGACTGG AAGAAGGAAA AAAAAGGTTG AGACCAATAA AATGGAAGAC 
CAGCTGAACA ATGAAGAAAT GAAAGGAGAT CTCAGCCAAA GCGATTCACA GATTTCCGCT 
GCTGACCCTC CACAGACAGT AGGTGTGCAT GCCAATAAAG ACTTAAGCCA GACTGAGTTA 
TCTCCGAAGA GTTCTCCTGT AACCCAGTCA AATAGCCAAA GCAAAGAGAG ACTGCCAGCT 
CTCTGCGAGT CTGAGAGTAT GTCGTTAGGT AGACAGGAGA CAAAAACACA ACTTGAAGAA 
TCCACAGAAA AGGAGACAAA TAATGACTCT CAAAACGCTT CATCTGCAGC GAGACCGAAG 
CATATACAGT CTACCACGTC TGACTCCGAG TCGGATAGCC AGCAAATTGT CCGTAAACGA 
AGATCGAGGA GATCTGAAAC TAAGTTACTG GAGATATCTG GTGTGCCGAT CAAAGAAGAC 
TTGAATCAGA CAGACTCCCA AACTCCAACA GGAGTGAAGG GAAGAGCTAT GAGGAGGAGA 
ACCGTAGAAG GTGCAGAGTC TAGTAAAGAT TCCACATCAG CGGTAAATAC AGAGGAGTTA 
AGTGCAGGCA AGGAGTCATC ACAAGAGCTT GGTAGATACA GAACTCGACG TTCTAGGGGT 
TTGTTGTCTC CTGAGAGTAT GGAGTCTGAA AACTCTGATA GCCGAGAAGA TGGGGCAAGG 
CCTAAAAAGA GGCTGCGAAA ACCACCATCA ACAGAGGTGT TACCTGTTGC TTCAGAACCC 
AAAGTTATTG AGATGGTACC AGAAAAGGAC GATGTTCAGT CAGAGGTTTT AATGGAGACG 
TCAAAGGATG AGGCAGATCC TGAAAATATC TCGAATACTT CAGTCAGTGA AACCAAAGAA 
CTTGAGCAAA AGAATAAAGA GGAAGTCCCT GCCCCTTGTG ATGCACCACA TGAACAGATT 
ATCACGGAAA AAGCAGCATC AAACAACAAA ATTGACACTG ACTTAACTGA TACAGACGAA 
AAAGAAACCG TCAAGGCAGA GGGTGAAGAA GCTAATGAAT CTTCGCAAAC TGGATCAGAA 
GATTTTAAAA AGACAGATGT TTTGCATAAA TGCCCACATA CAAAGGGACG GGGCCGTGCA 
CGTAGACGAT CTAAAAACTG CAACTGCTTT TCCAACAACC GTGATGTGTT TTCACAGGAC 
AGTGACTTCC AAGAATCACA AGATCTAAAA AATGAACAGG TTCCACTTGC TGCCAACATC 
TTGAACTCTC AACCTGATCA GTCAAGCCCA TCAACTGCAT CAGAAGAAAA CAAAAGCTCA 
GCAATTGAAG AAGTCCCTGT TGCGGATGTT CCATCACTTT CAGCCTTATC AGCTGATCTC 
CCATCTGAGG TTTCACCTTC TGAAAGTCCC ATTACAAAAC TTGGGGTAAT TGTTTCTCAG 
CTACCAGAAG TGGAGAAAGA AGCACAGCCT GGATCATCCA AACCAATGAA GGATTCGAGC 
AAGGAGCTTG TAGAAGAAGT GAATCGATTA AGTTCTGGAT CCACAGGTGG TCAAAAGCAC 
AAAGGAGAAA ATAGATTAGA AAACACTTCA GTAAGCCAAG AACCTGTCCA GAGTTCAACA 
CCTCAAGACA CGTCTCCAAG TCAAGGTCGT CCAGTGGACG ATGCACCAAT ATGCCAGGAG 
CAGTTAGAGT CTTCTCATGT CCAGCAGGAA GATGCAGTTA ACCCAGCAGT GATGGAAAGA 
GAAGACCTTG CTGTGTCAGA GCAGAGACAA GAGAAGGCTG TGTTGTCTGA AGAGTTAGAT 
GGAAAAGGTT TGCTTGACAA GGAAACTACT CTTGTTGAAA AGGAAGAAGA GATGCAGGTT 
AATAACCAGG ATCTTCCTGT GGATGTCCAA GAAGAGTTAC CTGTAGTGTC CCAGAGTAGC 
ACATCTGAAG CCTGCTTGGA TTCTCCACCC AAGCCAAAAC TGTTAAGCGC ACTCAGTGGT 
GAACTAGAGC CTGGCCAGAG TCCAACTAGA AGTCGGTCTC GAGTCTGGTC ACCATCTGCT 
TCTCCGTCTA CCAGTATTCT GAAGAAGGGT CAGAAGAGAG CATGTGAAGA GGAGACTCCA 
TCCCCCATTC CCAAGTCACG TCGAGTGTCT TTTGCAACTC CAATTTACCA GCAAGAACTG 
GCTGATGACA TTGATCGTCG TAGTCCGGTT ATTCGCACAA GTTCACCTAG GTCAAAAGTC 
TTGAGTGGAC AGTCAAAGTA TATCACTACA CCAACAAAAG GTGGCTATAC AAGTCTGAGT 
CCACGTAACC TCCGTAGCCC TGGATCCAAA AGCTCTAAAA AATGTCTGAT TTCAGAGATG 
AGCCAGGAGC CACGTCCTAT TCCTAAAGAT TGTGTTTATC CAGCACTTGT TGGCTGCTCT 
ACTCCAGTAG AAGCTGTTCT GCCACAGATT TCCTCCAACA TGTGGCCGCG TGGCTTCGGC 
CAGCTTGTTC GAGCCAGGAA CATTAAGACA GTAGGAGATC TGAGTGCTCT TACCCCTAAT 
GAAATCAAGT CTCTTCCTAT TCGCTCACCT AAGCTGTCCA ATGTGAGGAA AGCACTTAAA 
ACATATCATG AACAACAGAG AAAAGGACGT TCGGATGACC TGAAGAGTTT TGACGAAATG 
GAGAAAATGA CGTCTGAACC TGAAGAGATG GAGCTTCCTC AAAACCAAGA TGAGGAGCAA 
ACATCAGGAC CAGAGGCTCA AACAGCAGAA TCAGAGACTC AAGAAGACAA AGCCTCAGAA 
GTGGAAGTAA ATTCAGAAGT GGTGACTGAA GAGAACCAAT CTCATGATTT GCTCTCAGAT 
ATTGTAGCTC TTGGTGAACG CTTTACGCCT GAGGAACTCA GTCAGCATTC AGCCAGTCAG 
TTGGGTCTGA TGCATGAACA TCTGAGTGGA ATGATGAGGA GCATTGTGAC CAATCTGCAG 
TCCCGCTTGC TCAGTAATCT GGACGATAGC TTGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_009304632.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302907.1 Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X2, mRNA.

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CloneID	ODa15091
Clone ID Related Accession (Same CDS sequence)	XM_005167575.3 , XM_005167575.4 , XM_009304632.2
Accession Version	XM_009304632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	telomere-associated protein RIF1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CAGTGCCTTT ATCCAGTACC AGCCTCCTTC CTCTTCTGGA GTGTTTGGAA 
GATGAATCAG CTGGGCTTTC AGCGCAGACG GATGCCTACA TCACCATTGC AAACCGACTT 
AATGGAGAAG AAGGACGCCA GTTTCTGCCT GTGGTTGTTA AGCATTTTGC ACGTCTTAGT 
AAAGTGCTTT TGGTTCACAT CTCCAGTGAG AATGAGGAGC TGTGTCAGGC TGCACTGCAG 
GCTTTGGGAT TCTGTGTCTT CCACTCACAC ATAGTTTCAG TCATACCAGC AAATATTTCA 
GAGAATATAT TGTCTACGCT CTGCAATGTT GTGGTAAAGT CCAAAGAGAA GTTTACGTGC 
ACTCGGGCAT TGTGGGTCAT TTCAAAACAG AATTTCCCTC CAGAGGTGGC ATCCAAAATG 
GCCCCAGAGA TTCTCAAGAG CCTAGAAGCG ATGCAGACCA GAGAGGTGCA GTCCATCCTC 
ATTGACCACG AGTCACTCAA TGTCATCATA AGGTTACTGG AGCAGGCCCC TGTTCCGATG 
GCTGTTGGTG CTGTTTGCTG GGCTAAACTT GTTATTCCTC TGGTGGTTCA TTCGGCCAAT 
AAAGTGCGTC TGCGAGCGGC AGCTGCCCTG GAGCTCGGCA TGCCTCTTCT TCTGGAGAAA 
CAGCAGGAAG TGGCAGCCAT TGTTGAGCCA ATGATGTCTT CTGTGCTCAT CCCTGAAATG 
CAGAAGCTCT TTGCGTCTAA GAACGAGACA AACATGTTGA AACTCTGGCC TTTGTTTGTA 
AGACTTCTGG GCAAGTTACT CCACAAAGGC GGTGCTTTCA TTAACTCCTT GCTGTATCTA 
GAGGAGCTGG GTTTCCGCAA CTCTTCTCCT AATATTAAGA AGATTGCTTT CATAGCTTGG 
AAGAGCCTCA TTGACAATTT TGCCCTTAAT CCAGATATCC TGTGCAGTAG CAAGCGTCTG 
AAGCTCCTCA TGCAGCCGCT CAGCTCCATC CAAGTCAGGA CTGAAGCTCT TCTGCTTACC 
AAGCTGGAGG TCTGGTGGTA TCTTGCTGTC AAGCTCGGAC CCAACCTGTC TGCTAACTTT 
GAACAGGTTG GCGTGCCTCT ATTGCACAGC ACACTTCCTT CTGATTCTCC ACTTCAGAGC 
CCGACTACAC CTGCTCGAAC CTCTAACCCA AGCAACGGCA CACCTAACAC CCCTAAGTCA 
GGCATTCCCT CCTGCAGCAC GCCGAGCACG ACTCCTCGCA TGAATCTGAA CAGCAGCATG 
CAGGCGCCTC AGTCCTATCG TTCCATTCAG CTCCTGGGAC TAGAGATGCT CCTGCACTGG 
CTTGTGGGTC CAGAGGTCAC AGTCACAGCT GCTCGAGAAA ACCTTCAGCT CAGCCTGGAG 
CCCCTGACGC ACCCGCTTTT TACCAGCCCT TCCACCTTCA GCAGACATGC TTCTACCCTC 
ATATCTGCCA GCAAAGAAGG CATCATTACT GTAGGGAAGC ATGCTCCAGA AGCACTTCTT 
AATCTTATTT GGAGCAACCT GATCATTTCT GTCAGTGCAG CAATTGAAGC TGGAAATAAA 
AAGGAGCGAC AGGGTTCAGA GGTCCTCACA CTTTTCCTGC AGGCATTGCA GTCCATTTTG 
TCATCAGACG CTCTGCCTCC TGAACGTGCA CTGCTTCTGT TGGAAGCCAC TGTAAAGGGA 
ATACCTCAGA AGGTGCTTGG TTCTGCAGCT TATCAAGTGG CAAACATGGA CGTGTTAAAC 
GGTACTCCTG CACTTTTCCT AATACTGCTG TTCTATAAAA GTAACCAGCT CTCATCATTT 
CTTGAAGACG AAAGATTTTT TTCAAGCTTG GAGACCCTTG TAAGCTGTGG GTTTTGTGGA 
CCCACATCGC CCTTGGCATT TGGAGAGGCG GTTTTAGGAG CAGTTAATGG GAGTGTGGAG 
ACTGTTAAAA ACAAGAAGCA GCTCTGGAGA ATGTGGAGTA TGGTTGTAAG TCCTCTGACG 
GACACAATTA CTCAGACCAA TGAAGTCAAC CAAGGCGATG CTCTGGAGCA TAACTTCAGT 
GCCATTTACA GTGCGTTGAT GTTCCCCGTC ATCCACCTGC TGCCTTATTC AGCCTTACCA 
CAGACCACTC AGAAGGCCAT GATCAGCACA TGGTCCAGGC TTTACAAGGC TTTTGCCCGT 
TGCTCAGCTT TGGTTGCCAC AGCAGAGGAG AATACATGCT GTGAAGAGTT GTGTGTCAAG 
ATCTCTGCCT CTCTTGACCG TGAAGCTCTA AAAAGTCTGT CTATGCTAGA TGCAATAGCA 
CACATCTTGC TCATCATCGT TGAGAGTATG GACTTTTCTC CATATACCCC TCAGTTCCAG 
CAGAAGATGA AATCTCCTCG TACACCCATG AGTTGGGTAC GCAAGAGAAG CAGAGCCCTC 
GGGAATCTCT CAACCTTCCA CACTCTTCTG ATACAGACTC TAGAGGCTTT TCTCACTGCA 
GACCATTCAG AAGAATCTGC AGAGCCTTTA AATGGAATTG GCTCTGCCTT GGTGTCAATC 
TTGACCTCTC TTTTCACTAA TATTACCCTT CCCTCATTCT TACAAGAGGT GCTCTCCAGC 
CTCACCAAAC CACTTGCACG TCTTTATGAG CAGTTCTCCT GTTGTGATGA CCCATCAAAG 
CTCAGTCCAG AACTAGGGCA CAAGCTGGAG AAACTGGCCG CAGACTTGCT AGGCTGCCTT 
CAGACACATT TGACTCTCCA TGATGATGAA TTCCTCACTG TGTTGTCGCC CTTGCTTTGT 
GTACTTTTTT TGCACAAGAG CAAACAGGTT TGCTCTGTGG TCACTCAATT CTGGAATGCC 
ACTTTTGGAA ATGCGCTCAC CTTGAGTTAC CCAGAACCTC TGAAGTGTGT TTTAAATCAG 
GCTAAGCAGA AGACTCCACT GATTTTACCT GGTTTTGAAG TTGTTGATGT ACCAGATGAT 
TACAGTGGAA CATATATGAG TGAGAGTTCT CAGCTGGAGC CTCAGATTAG CGGGGTAAAA 
GTGACCTCTG TGGGAAAGAG GGACTCTCTT TTGGCAAGAG CAGAAGAGCT AACAGGAAAA 
AGTTCTGTAA CCACATCAAA ACCAGTGTCT GTAAAGCTGG ACTTTGGCTC CCCCAAGCCC 
TTAAGACGAG AAGCTCTTGA GGAGGAAGCA TCTGAAGATT TTGTTTTTAT TCCACCTGAG 
ATGAAAGAGA GAGTCCTGAC TGAGCATCAG AAGGAAGTGA AAAGGACTAA ACGGGTTGAC 
ATTCCTGCCC TGTACAATAA TCTTGATGCC TCCATGGACA CCACAGTCTT TTCACAGTAT 
ACTCAGAGTC AGGAGGAGTC AATGGACACA TTGCCTAAAG GTGAGAAGGA TCATCCTGTG 
GAAGCGGCTA GTGTTCAACT GAGGGAGGTT GCCTCAAATG AAGTACCAAC CACAGATGTT 
AAAGTCCAAA AAGTCACCAG TTCTGATATA GAAACTCCAG ATAAAGCTAC GCCGAATAAA 
GATCAAACTT TGGAGATGGA TCAATCACCA TCTACTGGTG TCTCAGTTGA TGTGAGTGGG 
CTTGAGAAAA GCGCATTGGA GGGGACAAGT CCTAATATTT CAAGTTCATC TGATGTGGTA 
TCTGGCACAC CTCCTAAACC TAACAGCAGG CGTCAGTCAT TTATAACCTT AGAAAAATAT 
GCTGAAGGGA AATCGACAAG TCCTGCAAGT CCTGCATTTA CAGGTACGCT CACCCGGAGA 
TCAAGAAGAC TGGAGGCTTC ACAGGCATCA ACATCAAATC AAAATGATTC TCAACCTCAG 
ATTAAAAAAG TGGATTCTCA GGAATCTCAA AAGATGAGCA GCATTGACAT GTCCATTCAA 
AATGAGCATG CTAGCATGGA AGTAAAAGAT GAGAAGTCAG TGGGAGCTAA TCCATGTGAA 
GGTACAGATG AGGAAGATGT TGTTCCAGAT ACCCAAACCC AAGTATCTGG TGACACATTG 
GGTATTAAAA GCAGTCCTGT GTCTAAAGCC CATGCGGAGG CACAGGGGAC TCCTGAAAAA 
AATTCACAGA GTCCTAATGA TTCTCTAGCT AGTCAAGAAC CAAGAAGGTC TGGCAGAAGG 
CGAAGTAAAC CTGTTCGTCC AGGGCAAGAT GCAGAGGAAA TAAAAAGTAA ATCTCAAGAA 
CAAGCCAGTC AACCTGCAGA CTCTGCGATA ATAAACTCAC AAAAGCCCCT TCTTGCTTCA 
CCTAGTAGTA CTTTGACTGG AAGAAGGAAA AAAAAGGTTG AGACCAATAA AATGGAAGAC 
CAGCTGAACA ATGAAGAAAT GAAAGGAGAT CTCAGCCAAA GCGATTCACA GATTTCCGCT 
GCTGACCCTC CACAGACAGT AGGTGTGCAT GCCAATAAAG ACTTAAGCCA GACTGAGTTA 
TCTCCGAAGA GTTCTCCTGT AACCCAGTCA AATAGCCAAA GCAAAGAGAG ACTGCCAGCT 
CTCTGCGAGT CTGAGAGTAT GTCGTTAGGT AGACAGGAGA CAAAAACACA ACTTGAAGAA 
TCCACAGAAA AGGAGACAAA TAATGACTCT CAAAACGCTT CATCTGCAGC GAGACCGAAG 
CATATACAGT CTACCACGTC TGACTCCGAG TCGGATAGCC AGCAAATTGT CCGTAAACGA 
AGATCGAGGA GATCTGAAAC TAAGTTACTG GAGATATCTG GTGTGCCGAT CAAAGAAGAC 
TTGAATCAGA CAGACTCCCA AACTCCAACA GGAGTGAAGG GAAGAGCTAT GAGGAGGAGA 
ACCGTAGAAG GTGCAGAGTC TAGTAAAGAT TCCACATCAG CGGTAAATAC AGAGGAGTTA 
AGTGCAGGCA AGGAGTCATC ACAAGAGCTT GGTAGATACA GAACTCGACG TTCTAGGGGT 
TTGTTGTCTC CTGAGAGTAT GGAGTCTGAA AACTCTGATA GCCGAGAAGA TGGGGCAAGG 
CCTAAAAAGA GGCTGCGAAA ACCACCATCA ACAGAGGTGT TACCTGTTGC TTCAGAACCC 
AAAGTTATTG AGATGGTACC AGAAAAGGAC GATGTTCAGT CAGAGGTTTT AATGGAGACG 
TCAAAGGATG AGGCAGATCC TGAAAATATC TCGAATACTT CAGTCAGTGA AACCAAAGAA 
CTTGAGCAAA AGAATAAAGA GGAAGTCCCT GCCCCTTGTG ATGCACCACA TGAACAGATT 
ATCACGGAAA AAGCAGCATC AAACAACAAA ATTGACACTG ACTTAACTGA TACAGACGAA 
AAAGAAACCG TCAAGGCAGA GGGTGAAGAA GCTAATGAAT CTTCGCAAAC TGGATCAGAA 
GATTTTAAAA AGACAGATGT TTTGCATAAA TGCCCACATA CAAAGGGACG GGGCCGTGCA 
CGTAGACGAT CTAAAAACTG CAACTGCTTT TCCAACAACC GTGATGTGTT TTCACAGGAC 
AGTGACTTCC AAGAATCACA AGATCTAAAA AATGAACAGG TTCCACTTGC TGCCAACATC 
TTGAACTCTC AACCTGATCA GTCAAGCCCA TCAACTGCAT CAGAAGAAAA CAAAAGCTCA 
GCAATTGAAG AAGTCCCTGT TGCGGATGTT CCATCACTTT CAGCCTTATC AGCTGATCTC 
CCATCTGAGG TTTCACCTTC TGAAAGTCCC ATTACAAAAC TTGGGGTAAT TGTTTCTCAG 
CTACCAGAAG TGGAGAAAGA AGCACAGCCT GGATCATCCA AACCAATGAA GGATTCGAGC 
AAGGAGCTTG TAGAAGAAGT GAATCGATTA AGTTCTGGAT CCACAGGTGG TCAAAAGCAC 
AAAGGAGAAA ATAGATTAGA AAACACTTCA GTAAGCCAAG AACCTGTCCA GAGTTCAACA 
CCTCAAGACA CGTCTCCAAG TCAAGGTCGT CCAGTGGACG ATGCACCAAT ATGCCAGGAG 
CAGTTAGAGT CTTCTCATGT CCAGCAGGAA GATGCAGTTA ACCCAGCAGT GATGGAAAGA 
GAAGACCTTG CTGTGTCAGA GCAGAGACAA GAGAAGGCTG TGTTGTCTGA AGAGTTAGAT 
GGAAAAGGTT TGCTTGACAA GGAAACTACT CTTGTTGAAA AGGAAGAAGA GATGCAGGTT 
AATAACCAGG ATCTTCCTGT GGATGTCCAA GAAGAGTTAC CTGTAGTGTC CCAGAGTAGC 
ACATCTGAAG CCTGCTTGGA TTCTCCACCC AAGCCAAAAC TGTTAAGCGC ACTCAGTGGT 
GAACTAGAGC CTGGCCAGAG TCCAACTAGA AGTCGGTCTC GAGTCTGGTC ACCATCTGCT 
TCTCCGTCTA CCAGTATTCT GAAGAAGGGT CAGAAGAGAG CATGTGAAGA GGAGACTCCA 
TCCCCCATTC CCAAGTCACG TCGAGTGTCT TTTGCAACTC CAATTTACCA GCAAGAACTG 
GCTGATGACA TTGATCGTCG TAGTCCGGTT ATTCGCACAA GTTCACCTAG GTCAAAAGTC 
TTGAGTGGAC AGTCAAAGTA TATCACTACA CCAACAAAAG GTGGCTATAC AAGTCTGAGT 
CCACGTAACC TCCGTAGCCC TGGATCCAAA AGCTCTAAAA AATGTCTGAT TTCAGAGATG 
AGCCAGGAGC CACGTCCTAT TCCTAAAGAT TGTGTTTATC CAGCACTTGT TGGCTGCTCT 
ACTCCAGTAG AAGCTGTTCT GCCACAGATT TCCTCCAACA TGTGGCCGCG TGGCTTCGGC 
CAGCTTGTTC GAGCCAGGAA CATTAAGACA GTAGGAGATC TGAGTGCTCT TACCCCTAAT 
GAAATCAAGT CTCTTCCTAT TCGCTCACCT AAGCTGTCCA ATGTGAGGAA AGCACTTAAA 
ACATATCATG AACAACAGAG AAAAGGACGT TCGGATGACC TGAAGAGTTT TGACGAAATG 
GAGAAAATGA CGTCTGAACC TGAAGAGATG GAGCTTCCTC AAAACCAAGA TGAGGAGCAA 
ACATCAGGAC CAGAGGCTCA AACAGCAGAA TCAGAGACTC AAGAAGACAA AGCCTCAGAA 
GTGGAAGTAA ATTCAGAAGT GGTGACTGAA GAGAACCAAT CTCATGATTT GCTCTCAGAT 
ATTGTAGCTC TTGGTGAACG CTTTACGCCT GAGGAACTCA GTCAGCATTC AGCCAGTCAG 
TTGGGTCTGA TGCATGAACA TCTGAGTGGA ATGATGAGGA GCATTGTGAC CAATCTGCAG 
TCCCGCTTGC TCAGTAATCT GGACGATAGC TTGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302907.1
CDS	286..7464
Translation	MMAAVPLSSTSLLPLLECLEDESAGLSAQTDAYITIANRLNGEEGRQFLPVVVKHFARLSKVLLVHISSENEELCQAALQALGFCVFHSHIVSVIPANISENILSTLCNVVVKSKEKFTCTRALWVISKQNFPPEVASKMAPEILKSLEAMQTREVQSILIDHESLNVIIRLLEQAPVPMAVGAVCWAKLVIPLVVHSANKVRLRAAAALELGMPLLLEKQQEVAAIVEPMMSSVLIPEMQKLFASKNETNMLKLWPLFVRLLGKLLHKGGAFINSLLYLEELGFRNSSPNIKKIAFIAWKSLIDNFALNPDILCSSKRLKLLMQPLSSIQVRTEALLLTKLEVWWYLAVKLGPNLSANFEQVGVPLLHSTLPSDSPLQSPTTPARTSNPSNGTPNTPKSGIPSCSTPSTTPRMNLNSSMQAPQSYRSIQLLGLEMLLHWLVGPEVTVTAARENLQLSLEPLTHPLFTSPSTFSRHASTLISASKEGIITVGKHAPEALLNLIWSNLIISVSAAIEAGNKKERQGSEVLTLFLQALQSILSSDALPPERALLLLEATVKGIPQKVLGSAAYQVANMDVLNGTPALFLILLFYKSNQLSSFLEDERFFSSLETLVSCGFCGPTSPLAFGEAVLGAVNGSVETVKNKKQLWRMWSMVVSPLTDTITQTNEVNQGDALEHNFSAIYSALMFPVIHLLPYSALPQTTQKAMISTWSRLYKAFARCSALVATAEENTCCEELCVKISASLDREALKSLSMLDAIAHILLIIVESMDFSPYTPQFQQKMKSPRTPMSWVRKRSRALGNLSTFHTLLIQTLEAFLTADHSEESAEPLNGIGSALVSILTSLFTNITLPSFLQEVLSSLTKPLARLYEQFSCCDDPSKLSPELGHKLEKLAADLLGCLQTHLTLHDDEFLTVLSPLLCVLFLHKSKQVCSVVTQFWNATFGNALTLSYPEPLKCVLNQAKQKTPLILPGFEVVDVPDDYSGTYMSESSQLEPQISGVKVTSVGKRDSLLARAEELTGKSSVTTSKPVSVKLDFGSPKPLRREALEEEASEDFVFIPPEMKERVLTEHQKEVKRTKRVDIPALYNNLDASMDTTVFSQYTQSQEESMDTLPKGEKDHPVEAASVQLREVASNEVPTTDVKVQKVTSSDIETPDKATPNKDQTLEMDQSPSTGVSVDVSGLEKSALEGTSPNISSSSDVVSGTPPKPNSRRQSFITLEKYAEGKSTSPASPAFTGTLTRRSRRLEASQASTSNQNDSQPQIKKVDSQESQKMSSIDMSIQNEHASMEVKDEKSVGANPCEGTDEEDVVPDTQTQVSGDTLGIKSSPVSKAHAEAQGTPEKNSQSPNDSLASQEPRRSGRRRSKPVRPGQDAEEIKSKSQEQASQPADSAIINSQKPLLASPSSTLTGRRKKKVETNKMEDQLNNEEMKGDLSQSDSQISAADPPQTVGVHANKDLSQTELSPKSSPVTQSNSQSKERLPALCESESMSLGRQETKTQLEESTEKETNNDSQNASSAARPKHIQSTTSDSESDSQQIVRKRRSRRSETKLLEISGVPIKEDLNQTDSQTPTGVKGRAMRRRTVEGAESSKDSTSAVNTEELSAGKESSQELGRYRTRRSRGLLSPESMESENSDSREDGARPKKRLRKPPSTEVLPVASEPKVIEMVPEKDDVQSEVLMETSKDEADPENISNTSVSETKELEQKNKEEVPAPCDAPHEQIITEKAASNNKIDTDLTDTDEKETVKAEGEEANESSQTGSEDFKKTDVLHKCPHTKGRGRARRRSKNCNCFSNNRDVFSQDSDFQESQDLKNEQVPLAANILNSQPDQSSPSTASEENKSSAIEEVPVADVPSLSALSADLPSEVSPSESPITKLGVIVSQLPEVEKEAQPGSSKPMKDSSKELVEEVNRLSSGSTGGQKHKGENRLENTSVSQEPVQSSTPQDTSPSQGRPVDDAPICQEQLESSHVQQEDAVNPAVMEREDLAVSEQRQEKAVLSEELDGKGLLDKETTLVEKEEEMQVNNQDLPVDVQEELPVVSQSSTSEACLDSPPKPKLLSALSGELEPGQSPTRSRSRVWSPSASPSTSILKKGQKRACEEETPSPIPKSRRVSFATPIYQQELADDIDRRSPVIRTSSPRSKVLSGQSKYITTPTKGGYTSLSPRNLRSPGSKSSKKCLISEMSQEPRPIPKDCVYPALVGCSTPVEAVLPQISSNMWPRGFGQLVRARNIKTVGDLSALTPNEIKSLPIRSPKLSNVRKALKTYHEQQRKGRSDDLKSFDEMEKMTSEPEEMELPQNQDEEQTSGPEAQTAESETQEDKASEVEVNSEVVTEENQSHDLLSDIVALGERFTPEELSQHSASQLGLMHEHLSGMMRSIVTNLQSRLLSNLDDSLP

Target ORF information:

RefSeq Version	XM_009304632.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304632.2

ORF Insert Sequence:

ATGATGGCGG CAGTGCCTTT ATCCAGTACC AGCCTCCTTC CTCTTCTGGA GTGTTTGGAA 
GATGAATCAG CTGGGCTTTC AGCGCAGACG GATGCCTACA TCACCATTGC AAACCGACTT 
AATGGAGAAG AAGGACGCCA GTTTCTGCCT GTGGTTGTTA AGCATTTTGC ACGTCTTAGT 
AAAGTGCTTT TGGTTCACAT CTCCAGTGAG AATGAGGAGC TGTGTCAGGC TGCACTGCAG 
GCTTTGGGAT TCTGTGTCTT CCACTCACAC ATAGTTTCAG TCATACCAGC AAATATTTCA 
GAGAATATAT TGTCTACGCT CTGCAATGTT GTGGTAAAGT CCAAAGAGAA GTTTACGTGC 
ACTCGGGCAT TGTGGGTCAT TTCAAAACAG AATTTCCCTC CAGAGGTGGC ATCCAAAATG 
GCCCCAGAGA TTCTCAAGAG CCTAGAAGCG ATGCAGACCA GAGAGGTGCA GTCCATCCTC 
ATTGACCACG AGTCACTCAA TGTCATCATA AGGTTACTGG AGCAGGCCCC TGTTCCGATG 
GCTGTTGGTG CTGTTTGCTG GGCTAAACTT GTTATTCCTC TGGTGGTTCA TTCGGCCAAT 
AAAGTGCGTC TGCGAGCGGC AGCTGCCCTG GAGCTCGGCA TGCCTCTTCT TCTGGAGAAA 
CAGCAGGAAG TGGCAGCCAT TGTTGAGCCA ATGATGTCTT CTGTGCTCAT CCCTGAAATG 
CAGAAGCTCT TTGCGTCTAA GAACGAGACA AACATGTTGA AACTCTGGCC TTTGTTTGTA 
AGACTTCTGG GCAAGTTACT CCACAAAGGC GGTGCTTTCA TTAACTCCTT GCTGTATCTA 
GAGGAGCTGG GTTTCCGCAA CTCTTCTCCT AATATTAAGA AGATTGCTTT CATAGCTTGG 
AAGAGCCTCA TTGACAATTT TGCCCTTAAT CCAGATATCC TGTGCAGTAG CAAGCGTCTG 
AAGCTCCTCA TGCAGCCGCT CAGCTCCATC CAAGTCAGGA CTGAAGCTCT TCTGCTTACC 
AAGCTGGAGG TCTGGTGGTA TCTTGCTGTC AAGCTCGGAC CCAACCTGTC TGCTAACTTT 
GAACAGGTTG GCGTGCCTCT ATTGCACAGC ACACTTCCTT CTGATTCTCC ACTTCAGAGC 
CCGACTACAC CTGCTCGAAC CTCTAACCCA AGCAACGGCA CACCTAACAC CCCTAAGTCA 
GGCATTCCCT CCTGCAGCAC GCCGAGCACG ACTCCTCGCA TGAATCTGAA CAGCAGCATG 
CAGGCGCCTC AGTCCTATCG TTCCATTCAG CTCCTGGGAC TAGAGATGCT CCTGCACTGG 
CTTGTGGGTC CAGAGGTCAC AGTCACAGCT GCTCGAGAAA ACCTTCAGCT CAGCCTGGAG 
CCCCTGACGC ACCCGCTTTT TACCAGCCCT TCCACCTTCA GCAGACATGC TTCTACCCTC 
ATATCTGCCA GCAAAGAAGG CATCATTACT GTAGGGAAGC ATGCTCCAGA AGCACTTCTT 
AATCTTATTT GGAGCAACCT GATCATTTCT GTCAGTGCAG CAATTGAAGC TGGAAATAAA 
AAGGAGCGAC AGGGTTCAGA GGTCCTCACA CTTTTCCTGC AGGCATTGCA GTCCATTTTG 
TCATCAGACG CTCTGCCTCC TGAACGTGCA CTGCTTCTGT TGGAAGCCAC TGTAAAGGGA 
ATACCTCAGA AGGTGCTTGG TTCTGCAGCT TATCAAGTGG CAAACATGGA CGTGTTAAAC 
GGTACTCCTG CACTTTTCCT AATACTGCTG TTCTATAAAA GTAACCAGCT CTCATCATTT 
CTTGAAGACG AAAGATTTTT TTCAAGCTTG GAGACCCTTG TAAGCTGTGG GTTTTGTGGA 
CCCACATCGC CCTTGGCATT TGGAGAGGCG GTTTTAGGAG CAGTTAATGG GAGTGTGGAG 
ACTGTTAAAA ACAAGAAGCA GCTCTGGAGA ATGTGGAGTA TGGTTGTAAG TCCTCTGACG 
GACACAATTA CTCAGACCAA TGAAGTCAAC CAAGGCGATG CTCTGGAGCA TAACTTCAGT 
GCCATTTACA GTGCGTTGAT GTTCCCCGTC ATCCACCTGC TGCCTTATTC AGCCTTACCA 
CAGACCACTC AGAAGGCCAT GATCAGCACA TGGTCCAGGC TTTACAAGGC TTTTGCCCGT 
TGCTCAGCTT TGGTTGCCAC AGCAGAGGAG AATACATGCT GTGAAGAGTT GTGTGTCAAG 
ATCTCTGCCT CTCTTGACCG TGAAGCTCTA AAAAGTCTGT CTATGCTAGA TGCAATAGCA 
CACATCTTGC TCATCATCGT TGAGAGTATG GACTTTTCTC CATATACCCC TCAGTTCCAG 
CAGAAGATGA AATCTCCTCG TACACCCATG AGTTGGGTAC GCAAGAGAAG CAGAGCCCTC 
GGGAATCTCT CAACCTTCCA CACTCTTCTG ATACAGACTC TAGAGGCTTT TCTCACTGCA 
GACCATTCAG AAGAATCTGC AGAGCCTTTA AATGGAATTG GCTCTGCCTT GGTGTCAATC 
TTGACCTCTC TTTTCACTAA TATTACCCTT CCCTCATTCT TACAAGAGGT GCTCTCCAGC 
CTCACCAAAC CACTTGCACG TCTTTATGAG CAGTTCTCCT GTTGTGATGA CCCATCAAAG 
CTCAGTCCAG AACTAGGGCA CAAGCTGGAG AAACTGGCCG CAGACTTGCT AGGCTGCCTT 
CAGACACATT TGACTCTCCA TGATGATGAA TTCCTCACTG TGTTGTCGCC CTTGCTTTGT 
GTACTTTTTT TGCACAAGAG CAAACAGGTT TGCTCTGTGG TCACTCAATT CTGGAATGCC 
ACTTTTGGAA ATGCGCTCAC CTTGAGTTAC CCAGAACCTC TGAAGTGTGT TTTAAATCAG 
GCTAAGCAGA AGACTCCACT GATTTTACCT GGTTTTGAAG TTGTTGATGT ACCAGATGAT 
TACAGTGGAA CATATATGAG TGAGAGTTCT CAGCTGGAGC CTCAGATTAG CGGGGTAAAA 
GTGACCTCTG TGGGAAAGAG GGACTCTCTT TTGGCAAGAG CAGAAGAGCT AACAGGAAAA 
AGTTCTGTAA CCACATCAAA ACCAGTGTCT GTAAAGCTGG ACTTTGGCTC CCCCAAGCCC 
TTAAGACGAG AAGCTCTTGA GGAGGAAGCA TCTGAAGATT TTGTTTTTAT TCCACCTGAG 
ATGAAAGAGA GAGTCCTGAC TGAGCATCAG AAGGAAGTGA AAAGGACTAA ACGGGTTGAC 
ATTCCTGCCC TGTACAATAA TCTTGATGCC TCCATGGACA CCACAGTCTT TTCACAGTAT 
ACTCAGAGTC AGGAGGAGTC AATGGACACA TTGCCTAAAG GTGAGAAGGA TCATCCTGTG 
GAAGCGGCTA GTGTTCAACT GAGGGAGGTT GCCTCAAATG AAGTACCAAC CACAGATGTT 
AAAGTCCAAA AAGTCACCAG TTCTGATATA GAAACTCCAG ATAAAGCTAC GCCGAATAAA 
GATCAAACTT TGGAGATGGA TCAATCACCA TCTACTGGTG TCTCAGTTGA TGTGAGTGGG 
CTTGAGAAAA GCGCATTGGA GGGGACAAGT CCTAATATTT CAAGTTCATC TGATGTGGTA 
TCTGGCACAC CTCCTAAACC TAACAGCAGG CGTCAGTCAT TTATAACCTT AGAAAAATAT 
GCTGAAGGGA AATCGACAAG TCCTGCAAGT CCTGCATTTA CAGGTACGCT CACCCGGAGA 
TCAAGAAGAC TGGAGGCTTC ACAGGCATCA ACATCAAATC AAAATGATTC TCAACCTCAG 
ATTAAAAAAG TGGATTCTCA GGAATCTCAA AAGATGAGCA GCATTGACAT GTCCATTCAA 
AATGAGCATG CTAGCATGGA AGTAAAAGAT GAGAAGTCAG TGGGAGCTAA TCCATGTGAA 
GGTACAGATG AGGAAGATGT TGTTCCAGAT ACCCAAACCC AAGTATCTGG TGACACATTG 
GGTATTAAAA GCAGTCCTGT GTCTAAAGCC CATGCGGAGG CACAGGGGAC TCCTGAAAAA 
AATTCACAGA GTCCTAATGA TTCTCTAGCT AGTCAAGAAC CAAGAAGGTC TGGCAGAAGG 
CGAAGTAAAC CTGTTCGTCC AGGGCAAGAT GCAGAGGAAA TAAAAAGTAA ATCTCAAGAA 
CAAGCCAGTC AACCTGCAGA CTCTGCGATA ATAAACTCAC AAAAGCCCCT TCTTGCTTCA 
CCTAGTAGTA CTTTGACTGG AAGAAGGAAA AAAAAGGTTG AGACCAATAA AATGGAAGAC 
CAGCTGAACA ATGAAGAAAT GAAAGGAGAT CTCAGCCAAA GCGATTCACA GATTTCCGCT 
GCTGACCCTC CACAGACAGT AGGTGTGCAT GCCAATAAAG ACTTAAGCCA GACTGAGTTA 
TCTCCGAAGA GTTCTCCTGT AACCCAGTCA AATAGCCAAA GCAAAGAGAG ACTGCCAGCT 
CTCTGCGAGT CTGAGAGTAT GTCGTTAGGT AGACAGGAGA CAAAAACACA ACTTGAAGAA 
TCCACAGAAA AGGAGACAAA TAATGACTCT CAAAACGCTT CATCTGCAGC GAGACCGAAG 
CATATACAGT CTACCACGTC TGACTCCGAG TCGGATAGCC AGCAAATTGT CCGTAAACGA 
AGATCGAGGA GATCTGAAAC TAAGTTACTG GAGATATCTG GTGTGCCGAT CAAAGAAGAC 
TTGAATCAGA CAGACTCCCA AACTCCAACA GGAGTGAAGG GAAGAGCTAT GAGGAGGAGA 
ACCGTAGAAG GTGCAGAGTC TAGTAAAGAT TCCACATCAG CGGTAAATAC AGAGGAGTTA 
AGTGCAGGCA AGGAGTCATC ACAAGAGCTT GGTAGATACA GAACTCGACG TTCTAGGGGT 
TTGTTGTCTC CTGAGAGTAT GGAGTCTGAA AACTCTGATA GCCGAGAAGA TGGGGCAAGG 
CCTAAAAAGA GGCTGCGAAA ACCACCATCA ACAGAGGTGT TACCTGTTGC TTCAGAACCC 
AAAGTTATTG AGATGGTACC AGAAAAGGAC GATGTTCAGT CAGAGGTTTT AATGGAGACG 
TCAAAGGATG AGGCAGATCC TGAAAATATC TCGAATACTT CAGTCAGTGA AACCAAAGAA 
CTTGAGCAAA AGAATAAAGA GGAAGTCCCT GCCCCTTGTG ATGCACCACA TGAACAGATT 
ATCACGGAAA AAGCAGCATC AAACAACAAA ATTGACACTG ACTTAACTGA TACAGACGAA 
AAAGAAACCG TCAAGGCAGA GGGTGAAGAA GCTAATGAAT CTTCGCAAAC TGGATCAGAA 
GATTTTAAAA AGACAGATGT TTTGCATAAA TGCCCACATA CAAAGGGACG GGGCCGTGCA 
CGTAGACGAT CTAAAAACTG CAACTGCTTT TCCAACAACC GTGATGTGTT TTCACAGGAC 
AGTGACTTCC AAGAATCACA AGATCTAAAA AATGAACAGG TTCCACTTGC TGCCAACATC 
TTGAACTCTC AACCTGATCA GTCAAGCCCA TCAACTGCAT CAGAAGAAAA CAAAAGCTCA 
GCAATTGAAG AAGTCCCTGT TGCGGATGTT CCATCACTTT CAGCCTTATC AGCTGATCTC 
CCATCTGAGG TTTCACCTTC TGAAAGTCCC ATTACAAAAC TTGGGGTAAT TGTTTCTCAG 
CTACCAGAAG TGGAGAAAGA AGCACAGCCT GGATCATCCA AACCAATGAA GGATTCGAGC 
AAGGAGCTTG TAGAAGAAGT GAATCGATTA AGTTCTGGAT CCACAGGTGG TCAAAAGCAC 
AAAGGAGAAA ATAGATTAGA AAACACTTCA GTAAGCCAAG AACCTGTCCA GAGTTCAACA 
CCTCAAGACA CGTCTCCAAG TCAAGGTCGT CCAGTGGACG ATGCACCAAT ATGCCAGGAG 
CAGTTAGAGT CTTCTCATGT CCAGCAGGAA GATGCAGTTA ACCCAGCAGT GATGGAAAGA 
GAAGACCTTG CTGTGTCAGA GCAGAGACAA GAGAAGGCTG TGTTGTCTGA AGAGTTAGAT 
GGAAAAGGTT TGCTTGACAA GGAAACTACT CTTGTTGAAA AGGAAGAAGA GATGCAGGTT 
AATAACCAGG ATCTTCCTGT GGATGTCCAA GAAGAGTTAC CTGTAGTGTC CCAGAGTAGC 
ACATCTGAAG CCTGCTTGGA TTCTCCACCC AAGCCAAAAC TGTTAAGCGC ACTCAGTGGT 
GAACTAGAGC CTGGCCAGAG TCCAACTAGA AGTCGGTCTC GAGTCTGGTC ACCATCTGCT 
TCTCCGTCTA CCAGTATTCT GAAGAAGGGT CAGAAGAGAG CATGTGAAGA GGAGACTCCA 
TCCCCCATTC CCAAGTCACG TCGAGTGTCT TTTGCAACTC CAATTTACCA GCAAGAACTG 
GCTGATGACA TTGATCGTCG TAGTCCGGTT ATTCGCACAA GTTCACCTAG GTCAAAAGTC 
TTGAGTGGAC AGTCAAAGTA TATCACTACA CCAACAAAAG GTGGCTATAC AAGTCTGAGT 
CCACGTAACC TCCGTAGCCC TGGATCCAAA AGCTCTAAAA AATGTCTGAT TTCAGAGATG 
AGCCAGGAGC CACGTCCTAT TCCTAAAGAT TGTGTTTATC CAGCACTTGT TGGCTGCTCT 
ACTCCAGTAG AAGCTGTTCT GCCACAGATT TCCTCCAACA TGTGGCCGCG TGGCTTCGGC 
CAGCTTGTTC GAGCCAGGAA CATTAAGACA GTAGGAGATC TGAGTGCTCT TACCCCTAAT 
GAAATCAAGT CTCTTCCTAT TCGCTCACCT AAGCTGTCCA ATGTGAGGAA AGCACTTAAA 
ACATATCATG AACAACAGAG AAAAGGACGT TCGGATGACC TGAAGAGTTT TGACGAAATG 
GAGAAAATGA CGTCTGAACC TGAAGAGATG GAGCTTCCTC AAAACCAAGA TGAGGAGCAA 
ACATCAGGAC CAGAGGCTCA AACAGCAGAA TCAGAGACTC AAGAAGACAA AGCCTCAGAA 
GTGGAAGTAA ATTCAGAAGT GGTGACTGAA GAGAACCAAT CTCATGATTT GCTCTCAGAT 
ATTGTAGCTC TTGGTGAACG CTTTACGCCT GAGGAACTCA GTCAGCATTC AGCCAGTCAG 
TTGGGTCTGA TGCATGAACA TCTGAGTGGA ATGATGAGGA GCATTGTGAC CAATCTGCAG 
TCCCGCTTGC TCAGTAATCT GGACGATAGC TTGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_009304633.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302908.1 Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X3, mRNA.

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CloneID	ODa45048
Clone ID Related Accession (Same CDS sequence)	XM_009304633.2 , XM_009304633.3
Accession Version	XM_009304633.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	telomere-associated protein RIF1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304633.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CAGTGCCTTT ATCCAGTACC AGCCTCCTTC CTCTTCTGGA GTGTTTGGAA 
GATGAATCAG CTGGGCTTTC AGCGCAGACG GATGCCTACA TCACCATTGC AAACCGACTT 
AATGGAGAAG AAGGACGCCA GTTTCTGCCT GTGGTTGTTA AGCATTTTGC ACGTCTTAGT 
AAAGTGCTTT TGGTTCACAT CTCCAGTGAG AATGAGGAGC TGTGTCAGGC TGCACTGCAG 
GCTTTGGGAT TCTGTGTCTT CCACTCACAC ATAGTTTCAG TCATACCAGC AAATATTTCA 
GAGAATATAT TGTCTACGCT CTGCAATGTT GTGGTAAAGT CCAAAGAGAA GTTTACGTGC 
ACTCGGGCAT TGTGGGTCAT TTCAAAACAG AATTTCCCTC CAGAGGTGGC ATCCAAAATG 
GCCCCAGAGA TTCTCAAGAG CCTAGAAGCG ATGCAGACCA GAGAGGTGCA GTCCATCCTC 
ATTGACCACG AGTCACTCAA TGTCATCATA AGGTTACTGG AGCAGGCCCC TGTTCCGATG 
GCTGTTGGTG CTGTTTGCTG GGCTAAACTT GTTATTCCTC TGGTGGTTCA TTCGGCCAAT 
AAAGTGCGTC TGCGAGCGGC AGCTGCCCTG GAGCTCGGCA TGCCTCTTCT TCTGGAGAAA 
CAGCAGGAAG TGGCAGCCAT TGTTGAGCCA ATGATGTCTT CTGTGCTCAT CCCTGAAATG 
CAGAAGCTCT TTGCGTCTAA GAACGAGACA AACATGTTGA AACTCTGGCC TTTGTTTGTA 
AGACTTCTGG GCAAGTTACT CCACAAAGGC GGTGCTTTCA TTAACTCCTT GCTGTATCTA 
GAGGAGCTGG GTTTCCGCAA CTCTTCTCCT AATATTAAGA AGATTGCTTT CATAGCTTGG 
AAGAGCCTCA TTGACAATTT TGCCCTTAAT CCAGATATCC TGTGCAGTAG CAAGCGTCTG 
AAGCTCCTCA TGCAGCCGCT CAGCTCCATC CAAGTCAGGA CTGAAGCTCT TCTGCTTACC 
AAGCTGGAGG TCTGGTGGTA TCTTGCTGTC AAGCTCGGAC CCAACCTGTC TGCTAACTTT 
GAACAGGTTG GCGTGCCTCT ATTGCACAGC ACACTTCCTT CTGATTCTCC ACTTCAGAGC 
CCGACTACAC CTGCTCGAAC CTCTAACCCA AGCAACGGCA CACCTAACAC CCCTAAGTCA 
GGCATTCCCT CCTGCAGCAC GCCGAGCACG ACTCCTCGCA TGAATCTGAA CAGCAGCATG 
CAGGCGCCTC AGTCCTATCG TTCCATTCAG CTCCTGGGAC TAGAGATGCT CCTGCACTGG 
CTTGTGGGTC CAGAGGTCAC AGTCACAGCT GCTCGAGAAA ACCTTCAGCT CAGCCTGGAG 
CCCCTGACGC ACCCGCTTTT TACCAGCCCT TCCACCTTCA GCAGACATGC TTCTACCCTC 
ATATCTGCCA GCAAAGAAGG CATCATTACT GTAGGGAAGC ATGCTCCAGA AGCACTTCTT 
AATCTTATTT GGAGCAACCT GATCATTTCT GTCAGTGCAG CAATTGAAGC TGGAAATAAA 
AAGGAGCGAC AGGGTTCAGA GGTCCTCACA CTTTTCCTGC AGGCATTGCA GTCCATTTTG 
TCATCAGACG CTCTGCCTCC TGAACGTGCA CTGCTTCTGT TGGAAGCCAC TGTAAAGGGA 
ATACCTCAGA AGGTGCTTGG TTCTGCAGCT TATCAAGTGG CAAACATGGA CGTGTTAAAC 
GGTACTCCTG CACTTTTCCT AATACTGCTG TTCTATAAAA GTAACCAGCT CTCATCATTT 
CTTGAAGACG AAAGATTTTT TTCAAGCTTG GAGACCCTTG TAAGCTGTGG GTTTTGTGGA 
CCCACATCGC CCTTGGCATT TGGAGAGGCG GTTTTAGGAG CAGTTAATGG GAGTGTGGAG 
ACTGTTAAAA ACAAGAAGCA GCTCTGGAGA ATGTGGAGTA TGGTTGTAAG TCCTCTGACG 
GACACAATTA CTCAGACCAA TGAAGTCAAC CAAGGCGATG CTCTGGAGCA TAACTTCAGT 
GCCATTTACA GTGCGTTGAT GTTCCCCGTC ATCCACCTGC TGCCTTATTC AGCCTTACCA 
CAGACCACTC AGAAGGCCAT GATCAGCACA TGGTCCAGGC TTTACAAGGC TTTTGCCCGT 
TGCTCAGCTT TGGTTGCCAC AGCAGAGGAG AATACATGCT GTGAAGAGTT GTGTGTCAAG 
ATCTCTGCCT CTCTTGACCG TGAAGCTCTA AAAAGTCTGT CTATGCTAGA TGCAATAGCA 
CACATCTTGC TCATCATCGT TGAGAGTATG GACTTTTCTC CATATACCCC TCAGTTCCAG 
CAGAAGATGA AATCTCCTCG TACACCCATG AGTTGGGTAC GCAAGAGAAG CAGAGCCCTC 
GGGAATCTCT CAACCTTCCA CACTCTTCTG ATACAGACTC TAGAGGCTTT TCTCACTGCA 
GACCATTCAG AAGAATCTGC AGAGCCTTTA AATGGAATTG GCTCTGCCTT GGTGTCAATC 
TTGACCTCTC TTTTCACTAA TATTACCCTT CCCTCATTCT TACAAGAGGT GCTCTCCAGC 
CTCACCAAAC CACTTGCACG TCTTTATGAG CAGTTCTCCT GTTGTGATGA CCCATCAAAG 
CTCAGTCCAG AACTAGGGCA CAAGCTGGAG AAACTGGCCG CAGACTTGCT AGGCTGCCTT 
CAGACACATT TGACTCTCCA TGATGATGAA TTCCTCACTG TGTTGTCGCC CTTGCTTTGT 
GTACTTTTTT TGCACAAGAG CAAACAGGTT TGCTCTGTGG TCACTCAATT CTGGAATGCC 
ACTTTTGGAA ATGCGCTCAC CTTGAGTTAC CCAGAACCTC TGAAGTGTGT TTTAAATCAG 
GCTAAGCAGA AGACTCCACT GATTTTACCT GGTTTTGAAG TTGTTGATGT ACCAGATGAT 
TACAGTGGAA CATATATGAG TGAGAGTTCT CAGCTGGAGC CTCAGATTAG CGGGGTAAAA 
GTGACCTCTG TGGGAAAGAG GGACTCTCTT TTGGCAAGAG CAGAAGAGCT AACAGGAAAA 
AGTTCTGTAA CCACATCAAA ACCAGTGTCT GTAAAGCTGG ACTTTGGCTC CCCCAAGCCC 
TTAAGACGAG AAGCTCTTGA GGAGGAAGCA TCTGAAGATT TTGTTTTTAT TCCACCTGAG 
ATGAAAGAGA GAGTCCTGAC TGAGCATCAG AAGGAAGTGA AAAGGACTAA ACGGGTTGAC 
ATTCCTGCCC TGTACAATAA TCTTGATGCC TCCATGGACA CCACAGTCTT TTCACAGTAT 
ACTCAGAGTC AGGAGGAGTC AATGGACACA TTGCCTAAAG GTGAGAAGGA TCATCCTGTG 
GAAGCGGCTA GTGTTCAACT GAGGGAGGTT GCCTCAAATG AAGTACCAAC CACAGATGTT 
AAAGTCCAAA AAGTCACCAG TTCTGATATA GAAACTCCAG ATAAAGCTAC GCCGAATAAA 
GATCAAACTT TGGAGATGGA TCAATCACCA TCTACTGGTG TCTCAGTTGA TGTGAGTGGG 
CTTGAGAAAA GCGCATTGGA GGGGACAAGT CCTAATATTT CAAGTTCATC TGATGTGGTA 
TCTGGCACAC CTCCTAAACC TAACAGCAGG CGTCAGTCAT TTATAACCTT AGAAAAATAT 
GCTGAAGGGA AATCGACAAG TCCTGCAAGT CCTGCATTTA CAGGTACGCT CACCCGGAGA 
TCAAGAAGAC TGGAGGCTTC ACAGGCATCA ACATCAAATC AAAATGATTC TCAACCTCAG 
ATTAAAAAAG TGGATTCTCA GGAATCTCAA AAGATGAGCA GCATTGACAT GTCCATTCAA 
AATGAGCATG CTAGCATGGA AGTAAAAGAT GAGAAGTCAG TGGGAGCTAA TCCATGTGAA 
GGTACAGATG AGGAAGATGT TGTTCCAGAT ACCCAAACCC AAGTATCTGG TGACACATTG 
GGTATTAAAA GCAGTCCTGT GTCTAAAGCC CATGCGGAGG CACAGGGGAC TCCTGAAAAA 
AATTCACAGA GTCCTAATGA TTCTCTAGCT AGTCAAGAAC CAAGAAGGTC TGGCAGAAGG 
CGAAGTAAAC CTGTTCGTCC AGGGCAAGAT GCAGAGGAAA TAAAAAGTAA ATCTCAAGAA 
CAAGCCAGTC AACCTGCAGA CTCTGCGATA ATAAACTCAC AAAAGCCCCT TCTTGCTTCA 
CCTAGTAGTA CTTTGACTGG AAGAAGGAAA AAAAAGGTTG AGACCAATAA AATGGAAGAC 
CAGCTGAACA ATGAAGAAAT GAAAGGAGAT CTCAGCCAAA GCGATTCACA GATTTCCGCT 
GCTGACCCTC CACAGACAGT AGGTGTGCAT GCCAATAAAG ACTTAAGCCA GACTGAGTTA 
TCTCCGAAGA GTTCTCCTGT AACCCAGTCA AATAGCCAAA GCAAAGAGAG ACTGCCAGCT 
CTCTGCGAGT CTGAGAGTAT GTCGTTAGGT AGACAGGAGA CAAAAACACA ACTTGAAGAA 
TCCACAGAAA AGGAGACAAA TAATGACTCT CAAAACGCTT CATCTGCAGC GAGACCGAAG 
CATATACAGT CTACCACGTC TGACTCCGAG TCGGATAGCC AGCAAATTGT CCGTAAACGA 
AGATCGAGGA GATCTGAAAC TAAGTTACTG GAGATATCTG GTGTGCCGAT CAAAGAAGAC 
TTGAATCAGA CAGACTCCCA AACTCCAACA GGAGTGAAGG GAAGAGCTAT GAGGAGGAGA 
ACCGTAGAAG GTGCAGAGTC TAGTAAAGAT TCCACATCAG CGGTAAATAC AGAGGAGTTA 
AGTGCAGGCA AGGAGTCATC ACAAGAGCTT GGTAGATACA GAACTCGACG TTCTAGGGGT 
TTGTTGTCTC CTGAGAGTAT GGAGTCTGAA AACTCTGATA GCCGAGAAGA TGGGGCAAGG 
CCTAAAAAGA GGCTGCGAAA ACCACCATCA ACAGAGGTGT TACCTGTTGC TTCAGAACCC 
AAAGTTATTG AGATGGTACC AGAAAAGGAC GATGTTCAGT CAGAGGTTTT AATGGAGACG 
TCAAAGGATG AGGCAGATCC TGAAAATATC TCGAATACTT CAGTCAGTGA AACCAAAGAA 
CTTGAGCAAA AGAATAAAGA GGAAGTCCCT GCCCCTTGTG ATGCACCACA TGAACAGATT 
ATCACGGAAA AAGCAGCATC AAACAACAAA ATTGACACTG ACTTAACTGA TACAGACGAA 
AAAGAAACCG TCAAGGCAGA GGGTGAAGAA GCTAATGAAT CTTCGCAAAC TGGATCAGAA 
GATTTTAAAA AGACAGATGT TTTGCATAAA TGCCCACATA CAAAGGGACG GGGCCGTGCA 
CGTAGACGAT CTAAAAACTG CAACTGCTTT TCCAACAACC GTGATGTGTT TTCACAGGAC 
AGTGACTTCC AAGAATCACA AGATCTAAAA AATGAACAGG TTCCACTTGC TGCCAACATC 
TTGAACTCTC AACCTGATCA GTCAAGCCCA TCAACTGCAT CAGAAGAAAA CAAAAGCTCA 
GCAATTGAAG AAGTCCCTGT TGCGGATGTT CCATCACTTT CAGCCTTATC AGCTGATCTC 
CCATCTGAGG TTTCACCTTC TGAAAGTCCC ATTACAAAAC TTGGGGTAAT TGTTTCTCAG 
CTACCAGAAG TGGAGAAAGA AGCACAGCCT GGATCATCCA AACCAATGAA GGATTCGAGC 
AAGGAGCTTG TAGAAGAAGT GAATCGATTA AGTTCTGGAT CCACAGGTGG TCAAAAGCAC 
AAAGGAGAAA ATAGATTAGA AAACACTTCA GTAAGCCAAG AACCTGTCCA GAGTTCAACA 
CCTCAAGACA CGTCTCCAAG TCAAGGTCGT CCAGTGGACG ATGCACCAAT ATGCCAGGAG 
CAGTTAGAGT CTTCTCATGT CCAGCAGGAA GATGCAGTTA ACCCAGCAGT GATGGAAAGA 
GAAGACCTTG CTGTGTCAGA GCAGAGACAA GAGAAGGCTG TGTTGTCTGA AGAGTTAGAT 
GGAAAAGGTT TGCTTGACAA GGAAACTACT CTTGTTGAAA AGGAAGAAGA GATGCAGGTT 
AATAACCAGG ATCTTCCTGT GGATGTCCAA GAAGAGTTAC CTGTAGTGTC CCAGAGTAGC 
ACATCTGAAG CCTGCTTGGA TTCTCCACCC AAGCCAAAAC TGTTAAGCGC ACTCAGTGGT 
GAACTAGAGC CTGGCCAGAG TCCAACTAGA AGTCGGTCTC GAGTCTGGTC ACCATCTGCT 
TCTCCGTCTA CCAGTATTCT GAAGAAGGGT CAGAAGAGAG CATGTGAAGA GGAGACTCCA 
TCCCCCATTC CCAAGTCACG TCGAGTGTCT TTTGCAACTC CAATTTACCA GCAAGAACTG 
GCTGATGACA TTGATCGTCG TAGTCCGGTT ATTCGCACAA GTTCACCTAG GTCAAAAGTC 
TTGAGTGGAC AGTCAAAGTA TATCACTACA CCAACAAAAG GTGGCTATAC AAGTCTGAGT 
CCACGTAACC TCCGTAGCCC TGGATCCAAA AGCTCTAAAA AATGTCTGAT TTCAGAGATG 
AGCCAGGAGC CACGTCCTAT TCCTAAAGAT TGTGTTTATC CAGCACTTGT TGGCTGCTCT 
ACTCCAGTAG AAGCTGTTCT GCCACAGATT TCCTCCAACA TGTGGCCGCG TGGCTTCGGC 
CAGCTTGTTC GAGCCAGGAA CATTAAGACA GTAGGAGATC TGAGTGCTCT TACCCCTAAT 
GAAATCAAGT CTCTTCCTAT TCGCTCACCT AAGCTGTCCA ATGTGAGGAA AGCACTTAAA 
ACATATCATG AACAACAGAG AAAAGGACGT TCGGATGACC TGAAGAGTTT TGACGAAATG 
GAGAAAATGA CGTCTGAACC TGAAGAGATG GAGCTTCCTC AAAACCAAGA TGAGGAGCAA 
ACATCAGGAC CAGAGGCTCA AACAGCAGAA TCAGAGACTC AAGACAAAGC CTCAGAAGTG 
GAAGTAAATT CAGAAGTGGT GACTGAAGAG AACCAATCTC ATGATTTGCT CTCAGATATT 
GTAGCTCTTG GTGAACGCTT TACGCCTGAG GAACTCAGTC AGCATTCAGC CAGTCAGTTG 
GGTCTGATGC ATGAACATCT GAGTGGAATG ATGAGGAGCA TTGTGACCAA TCTGCAGTCC 
CGCTTGCTCA GTAATCTGGA CGATAGCTTG CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302908.1
CDS	184..7359
Translation	MMAAVPLSSTSLLPLLECLEDESAGLSAQTDAYITIANRLNGEEGRQFLPVVVKHFARLSKVLLVHISSENEELCQAALQALGFCVFHSHIVSVIPANISENILSTLCNVVVKSKEKFTCTRALWVISKQNFPPEVASKMAPEILKSLEAMQTREVQSILIDHESLNVIIRLLEQAPVPMAVGAVCWAKLVIPLVVHSANKVRLRAAAALELGMPLLLEKQQEVAAIVEPMMSSVLIPEMQKLFASKNETNMLKLWPLFVRLLGKLLHKGGAFINSLLYLEELGFRNSSPNIKKIAFIAWKSLIDNFALNPDILCSSKRLKLLMQPLSSIQVRTEALLLTKLEVWWYLAVKLGPNLSANFEQVGVPLLHSTLPSDSPLQSPTTPARTSNPSNGTPNTPKSGIPSCSTPSTTPRMNLNSSMQAPQSYRSIQLLGLEMLLHWLVGPEVTVTAARENLQLSLEPLTHPLFTSPSTFSRHASTLISASKEGIITVGKHAPEALLNLIWSNLIISVSAAIEAGNKKERQGSEVLTLFLQALQSILSSDALPPERALLLLEATVKGIPQKVLGSAAYQVANMDVLNGTPALFLILLFYKSNQLSSFLEDERFFSSLETLVSCGFCGPTSPLAFGEAVLGAVNGSVETVKNKKQLWRMWSMVVSPLTDTITQTNEVNQGDALEHNFSAIYSALMFPVIHLLPYSALPQTTQKAMISTWSRLYKAFARCSALVATAEENTCCEELCVKISASLDREALKSLSMLDAIAHILLIIVESMDFSPYTPQFQQKMKSPRTPMSWVRKRSRALGNLSTFHTLLIQTLEAFLTADHSEESAEPLNGIGSALVSILTSLFTNITLPSFLQEVLSSLTKPLARLYEQFSCCDDPSKLSPELGHKLEKLAADLLGCLQTHLTLHDDEFLTVLSPLLCVLFLHKSKQVCSVVTQFWNATFGNALTLSYPEPLKCVLNQAKQKTPLILPGFEVVDVPDDYSGTYMSESSQLEPQISGVKVTSVGKRDSLLARAEELTGKSSVTTSKPVSVKLDFGSPKPLRREALEEEASEDFVFIPPEMKERVLTEHQKEVKRTKRVDIPALYNNLDASMDTTVFSQYTQSQEESMDTLPKGEKDHPVEAASVQLREVASNEVPTTDVKVQKVTSSDIETPDKATPNKDQTLEMDQSPSTGVSVDVSGLEKSALEGTSPNISSSSDVVSGTPPKPNSRRQSFITLEKYAEGKSTSPASPAFTGTLTRRSRRLEASQASTSNQNDSQPQIKKVDSQESQKMSSIDMSIQNEHASMEVKDEKSVGANPCEGTDEEDVVPDTQTQVSGDTLGIKSSPVSKAHAEAQGTPEKNSQSPNDSLASQEPRRSGRRRSKPVRPGQDAEEIKSKSQEQASQPADSAIINSQKPLLASPSSTLTGRRKKKVETNKMEDQLNNEEMKGDLSQSDSQISAADPPQTVGVHANKDLSQTELSPKSSPVTQSNSQSKERLPALCESESMSLGRQETKTQLEESTEKETNNDSQNASSAARPKHIQSTTSDSESDSQQIVRKRRSRRSETKLLEISGVPIKEDLNQTDSQTPTGVKGRAMRRRTVEGAESSKDSTSAVNTEELSAGKESSQELGRYRTRRSRGLLSPESMESENSDSREDGARPKKRLRKPPSTEVLPVASEPKVIEMVPEKDDVQSEVLMETSKDEADPENISNTSVSETKELEQKNKEEVPAPCDAPHEQIITEKAASNNKIDTDLTDTDEKETVKAEGEEANESSQTGSEDFKKTDVLHKCPHTKGRGRARRRSKNCNCFSNNRDVFSQDSDFQESQDLKNEQVPLAANILNSQPDQSSPSTASEENKSSAIEEVPVADVPSLSALSADLPSEVSPSESPITKLGVIVSQLPEVEKEAQPGSSKPMKDSSKELVEEVNRLSSGSTGGQKHKGENRLENTSVSQEPVQSSTPQDTSPSQGRPVDDAPICQEQLESSHVQQEDAVNPAVMEREDLAVSEQRQEKAVLSEELDGKGLLDKETTLVEKEEEMQVNNQDLPVDVQEELPVVSQSSTSEACLDSPPKPKLLSALSGELEPGQSPTRSRSRVWSPSASPSTSILKKGQKRACEEETPSPIPKSRRVSFATPIYQQELADDIDRRSPVIRTSSPRSKVLSGQSKYITTPTKGGYTSLSPRNLRSPGSKSSKKCLISEMSQEPRPIPKDCVYPALVGCSTPVEAVLPQISSNMWPRGFGQLVRARNIKTVGDLSALTPNEIKSLPIRSPKLSNVRKALKTYHEQQRKGRSDDLKSFDEMEKMTSEPEEMELPQNQDEEQTSGPEAQTAESETQDKASEVEVNSEVVTEENQSHDLLSDIVALGERFTPEELSQHSASQLGLMHEHLSGMMRSIVTNLQSRLLSNLDDSLP

Target ORF information:

RefSeq Version	XM_009304633.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304633.2

ORF Insert Sequence:

ATGATGGCGG CAGTGCCTTT ATCCAGTACC AGCCTCCTTC CTCTTCTGGA GTGTTTGGAA 
GATGAATCAG CTGGGCTTTC AGCGCAGACG GATGCCTACA TCACCATTGC AAACCGACTT 
AATGGAGAAG AAGGACGCCA GTTTCTGCCT GTGGTTGTTA AGCATTTTGC ACGTCTTAGT 
AAAGTGCTTT TGGTTCACAT CTCCAGTGAG AATGAGGAGC TGTGTCAGGC TGCACTGCAG 
GCTTTGGGAT TCTGTGTCTT CCACTCACAC ATAGTTTCAG TCATACCAGC AAATATTTCA 
GAGAATATAT TGTCTACGCT CTGCAATGTT GTGGTAAAGT CCAAAGAGAA GTTTACGTGC 
ACTCGGGCAT TGTGGGTCAT TTCAAAACAG AATTTCCCTC CAGAGGTGGC ATCCAAAATG 
GCCCCAGAGA TTCTCAAGAG CCTAGAAGCG ATGCAGACCA GAGAGGTGCA GTCCATCCTC 
ATTGACCACG AGTCACTCAA TGTCATCATA AGGTTACTGG AGCAGGCCCC TGTTCCGATG 
GCTGTTGGTG CTGTTTGCTG GGCTAAACTT GTTATTCCTC TGGTGGTTCA TTCGGCCAAT 
AAAGTGCGTC TGCGAGCGGC AGCTGCCCTG GAGCTCGGCA TGCCTCTTCT TCTGGAGAAA 
CAGCAGGAAG TGGCAGCCAT TGTTGAGCCA ATGATGTCTT CTGTGCTCAT CCCTGAAATG 
CAGAAGCTCT TTGCGTCTAA GAACGAGACA AACATGTTGA AACTCTGGCC TTTGTTTGTA 
AGACTTCTGG GCAAGTTACT CCACAAAGGC GGTGCTTTCA TTAACTCCTT GCTGTATCTA 
GAGGAGCTGG GTTTCCGCAA CTCTTCTCCT AATATTAAGA AGATTGCTTT CATAGCTTGG 
AAGAGCCTCA TTGACAATTT TGCCCTTAAT CCAGATATCC TGTGCAGTAG CAAGCGTCTG 
AAGCTCCTCA TGCAGCCGCT CAGCTCCATC CAAGTCAGGA CTGAAGCTCT TCTGCTTACC 
AAGCTGGAGG TCTGGTGGTA TCTTGCTGTC AAGCTCGGAC CCAACCTGTC TGCTAACTTT 
GAACAGGTTG GCGTGCCTCT ATTGCACAGC ACACTTCCTT CTGATTCTCC ACTTCAGAGC 
CCGACTACAC CTGCTCGAAC CTCTAACCCA AGCAACGGCA CACCTAACAC CCCTAAGTCA 
GGCATTCCCT CCTGCAGCAC GCCGAGCACG ACTCCTCGCA TGAATCTGAA CAGCAGCATG 
CAGGCGCCTC AGTCCTATCG TTCCATTCAG CTCCTGGGAC TAGAGATGCT CCTGCACTGG 
CTTGTGGGTC CAGAGGTCAC AGTCACAGCT GCTCGAGAAA ACCTTCAGCT CAGCCTGGAG 
CCCCTGACGC ACCCGCTTTT TACCAGCCCT TCCACCTTCA GCAGACATGC TTCTACCCTC 
ATATCTGCCA GCAAAGAAGG CATCATTACT GTAGGGAAGC ATGCTCCAGA AGCACTTCTT 
AATCTTATTT GGAGCAACCT GATCATTTCT GTCAGTGCAG CAATTGAAGC TGGAAATAAA 
AAGGAGCGAC AGGGTTCAGA GGTCCTCACA CTTTTCCTGC AGGCATTGCA GTCCATTTTG 
TCATCAGACG CTCTGCCTCC TGAACGTGCA CTGCTTCTGT TGGAAGCCAC TGTAAAGGGA 
ATACCTCAGA AGGTGCTTGG TTCTGCAGCT TATCAAGTGG CAAACATGGA CGTGTTAAAC 
GGTACTCCTG CACTTTTCCT AATACTGCTG TTCTATAAAA GTAACCAGCT CTCATCATTT 
CTTGAAGACG AAAGATTTTT TTCAAGCTTG GAGACCCTTG TAAGCTGTGG GTTTTGTGGA 
CCCACATCGC CCTTGGCATT TGGAGAGGCG GTTTTAGGAG CAGTTAATGG GAGTGTGGAG 
ACTGTTAAAA ACAAGAAGCA GCTCTGGAGA ATGTGGAGTA TGGTTGTAAG TCCTCTGACG 
GACACAATTA CTCAGACCAA TGAAGTCAAC CAAGGCGATG CTCTGGAGCA TAACTTCAGT 
GCCATTTACA GTGCGTTGAT GTTCCCCGTC ATCCACCTGC TGCCTTATTC AGCCTTACCA 
CAGACCACTC AGAAGGCCAT GATCAGCACA TGGTCCAGGC TTTACAAGGC TTTTGCCCGT 
TGCTCAGCTT TGGTTGCCAC AGCAGAGGAG AATACATGCT GTGAAGAGTT GTGTGTCAAG 
ATCTCTGCCT CTCTTGACCG TGAAGCTCTA AAAAGTCTGT CTATGCTAGA TGCAATAGCA 
CACATCTTGC TCATCATCGT TGAGAGTATG GACTTTTCTC CATATACCCC TCAGTTCCAG 
CAGAAGATGA AATCTCCTCG TACACCCATG AGTTGGGTAC GCAAGAGAAG CAGAGCCCTC 
GGGAATCTCT CAACCTTCCA CACTCTTCTG ATACAGACTC TAGAGGCTTT TCTCACTGCA 
GACCATTCAG AAGAATCTGC AGAGCCTTTA AATGGAATTG GCTCTGCCTT GGTGTCAATC 
TTGACCTCTC TTTTCACTAA TATTACCCTT CCCTCATTCT TACAAGAGGT GCTCTCCAGC 
CTCACCAAAC CACTTGCACG TCTTTATGAG CAGTTCTCCT GTTGTGATGA CCCATCAAAG 
CTCAGTCCAG AACTAGGGCA CAAGCTGGAG AAACTGGCCG CAGACTTGCT AGGCTGCCTT 
CAGACACATT TGACTCTCCA TGATGATGAA TTCCTCACTG TGTTGTCGCC CTTGCTTTGT 
GTACTTTTTT TGCACAAGAG CAAACAGGTT TGCTCTGTGG TCACTCAATT CTGGAATGCC 
ACTTTTGGAA ATGCGCTCAC CTTGAGTTAC CCAGAACCTC TGAAGTGTGT TTTAAATCAG 
GCTAAGCAGA AGACTCCACT GATTTTACCT GGTTTTGAAG TTGTTGATGT ACCAGATGAT 
TACAGTGGAA CATATATGAG TGAGAGTTCT CAGCTGGAGC CTCAGATTAG CGGGGTAAAA 
GTGACCTCTG TGGGAAAGAG GGACTCTCTT TTGGCAAGAG CAGAAGAGCT AACAGGAAAA 
AGTTCTGTAA CCACATCAAA ACCAGTGTCT GTAAAGCTGG ACTTTGGCTC CCCCAAGCCC 
TTAAGACGAG AAGCTCTTGA GGAGGAAGCA TCTGAAGATT TTGTTTTTAT TCCACCTGAG 
ATGAAAGAGA GAGTCCTGAC TGAGCATCAG AAGGAAGTGA AAAGGACTAA ACGGGTTGAC 
ATTCCTGCCC TGTACAATAA TCTTGATGCC TCCATGGACA CCACAGTCTT TTCACAGTAT 
ACTCAGAGTC AGGAGGAGTC AATGGACACA TTGCCTAAAG GTGAGAAGGA TCATCCTGTG 
GAAGCGGCTA GTGTTCAACT GAGGGAGGTT GCCTCAAATG AAGTACCAAC CACAGATGTT 
AAAGTCCAAA AAGTCACCAG TTCTGATATA GAAACTCCAG ATAAAGCTAC GCCGAATAAA 
GATCAAACTT TGGAGATGGA TCAATCACCA TCTACTGGTG TCTCAGTTGA TGTGAGTGGG 
CTTGAGAAAA GCGCATTGGA GGGGACAAGT CCTAATATTT CAAGTTCATC TGATGTGGTA 
TCTGGCACAC CTCCTAAACC TAACAGCAGG CGTCAGTCAT TTATAACCTT AGAAAAATAT 
GCTGAAGGGA AATCGACAAG TCCTGCAAGT CCTGCATTTA CAGGTACGCT CACCCGGAGA 
TCAAGAAGAC TGGAGGCTTC ACAGGCATCA ACATCAAATC AAAATGATTC TCAACCTCAG 
ATTAAAAAAG TGGATTCTCA GGAATCTCAA AAGATGAGCA GCATTGACAT GTCCATTCAA 
AATGAGCATG CTAGCATGGA AGTAAAAGAT GAGAAGTCAG TGGGAGCTAA TCCATGTGAA 
GGTACAGATG AGGAAGATGT TGTTCCAGAT ACCCAAACCC AAGTATCTGG TGACACATTG 
GGTATTAAAA GCAGTCCTGT GTCTAAAGCC CATGCGGAGG CACAGGGGAC TCCTGAAAAA 
AATTCACAGA GTCCTAATGA TTCTCTAGCT AGTCAAGAAC CAAGAAGGTC TGGCAGAAGG 
CGAAGTAAAC CTGTTCGTCC AGGGCAAGAT GCAGAGGAAA TAAAAAGTAA ATCTCAAGAA 
CAAGCCAGTC AACCTGCAGA CTCTGCGATA ATAAACTCAC AAAAGCCCCT TCTTGCTTCA 
CCTAGTAGTA CTTTGACTGG AAGAAGGAAA AAAAAGGTTG AGACCAATAA AATGGAAGAC 
CAGCTGAACA ATGAAGAAAT GAAAGGAGAT CTCAGCCAAA GCGATTCACA GATTTCCGCT 
GCTGACCCTC CACAGACAGT AGGTGTGCAT GCCAATAAAG ACTTAAGCCA GACTGAGTTA 
TCTCCGAAGA GTTCTCCTGT AACCCAGTCA AATAGCCAAA GCAAAGAGAG ACTGCCAGCT 
CTCTGCGAGT CTGAGAGTAT GTCGTTAGGT AGACAGGAGA CAAAAACACA ACTTGAAGAA 
TCCACAGAAA AGGAGACAAA TAATGACTCT CAAAACGCTT CATCTGCAGC GAGACCGAAG 
CATATACAGT CTACCACGTC TGACTCCGAG TCGGATAGCC AGCAAATTGT CCGTAAACGA 
AGATCGAGGA GATCTGAAAC TAAGTTACTG GAGATATCTG GTGTGCCGAT CAAAGAAGAC 
TTGAATCAGA CAGACTCCCA AACTCCAACA GGAGTGAAGG GAAGAGCTAT GAGGAGGAGA 
ACCGTAGAAG GTGCAGAGTC TAGTAAAGAT TCCACATCAG CGGTAAATAC AGAGGAGTTA 
AGTGCAGGCA AGGAGTCATC ACAAGAGCTT GGTAGATACA GAACTCGACG TTCTAGGGGT 
TTGTTGTCTC CTGAGAGTAT GGAGTCTGAA AACTCTGATA GCCGAGAAGA TGGGGCAAGG 
CCTAAAAAGA GGCTGCGAAA ACCACCATCA ACAGAGGTGT TACCTGTTGC TTCAGAACCC 
AAAGTTATTG AGATGGTACC AGAAAAGGAC GATGTTCAGT CAGAGGTTTT AATGGAGACG 
TCAAAGGATG AGGCAGATCC TGAAAATATC TCGAATACTT CAGTCAGTGA AACCAAAGAA 
CTTGAGCAAA AGAATAAAGA GGAAGTCCCT GCCCCTTGTG ATGCACCACA TGAACAGATT 
ATCACGGAAA AAGCAGCATC AAACAACAAA ATTGACACTG ACTTAACTGA TACAGACGAA 
AAAGAAACCG TCAAGGCAGA GGGTGAAGAA GCTAATGAAT CTTCGCAAAC TGGATCAGAA 
GATTTTAAAA AGACAGATGT TTTGCATAAA TGCCCACATA CAAAGGGACG GGGCCGTGCA 
CGTAGACGAT CTAAAAACTG CAACTGCTTT TCCAACAACC GTGATGTGTT TTCACAGGAC 
AGTGACTTCC AAGAATCACA AGATCTAAAA AATGAACAGG TTCCACTTGC TGCCAACATC 
TTGAACTCTC AACCTGATCA GTCAAGCCCA TCAACTGCAT CAGAAGAAAA CAAAAGCTCA 
GCAATTGAAG AAGTCCCTGT TGCGGATGTT CCATCACTTT CAGCCTTATC AGCTGATCTC 
CCATCTGAGG TTTCACCTTC TGAAAGTCCC ATTACAAAAC TTGGGGTAAT TGTTTCTCAG 
CTACCAGAAG TGGAGAAAGA AGCACAGCCT GGATCATCCA AACCAATGAA GGATTCGAGC 
AAGGAGCTTG TAGAAGAAGT GAATCGATTA AGTTCTGGAT CCACAGGTGG TCAAAAGCAC 
AAAGGAGAAA ATAGATTAGA AAACACTTCA GTAAGCCAAG AACCTGTCCA GAGTTCAACA 
CCTCAAGACA CGTCTCCAAG TCAAGGTCGT CCAGTGGACG ATGCACCAAT ATGCCAGGAG 
CAGTTAGAGT CTTCTCATGT CCAGCAGGAA GATGCAGTTA ACCCAGCAGT GATGGAAAGA 
GAAGACCTTG CTGTGTCAGA GCAGAGACAA GAGAAGGCTG TGTTGTCTGA AGAGTTAGAT 
GGAAAAGGTT TGCTTGACAA GGAAACTACT CTTGTTGAAA AGGAAGAAGA GATGCAGGTT 
AATAACCAGG ATCTTCCTGT GGATGTCCAA GAAGAGTTAC CTGTAGTGTC CCAGAGTAGC 
ACATCTGAAG CCTGCTTGGA TTCTCCACCC AAGCCAAAAC TGTTAAGCGC ACTCAGTGGT 
GAACTAGAGC CTGGCCAGAG TCCAACTAGA AGTCGGTCTC GAGTCTGGTC ACCATCTGCT 
TCTCCGTCTA CCAGTATTCT GAAGAAGGGT CAGAAGAGAG CATGTGAAGA GGAGACTCCA 
TCCCCCATTC CCAAGTCACG TCGAGTGTCT TTTGCAACTC CAATTTACCA GCAAGAACTG 
GCTGATGACA TTGATCGTCG TAGTCCGGTT ATTCGCACAA GTTCACCTAG GTCAAAAGTC 
TTGAGTGGAC AGTCAAAGTA TATCACTACA CCAACAAAAG GTGGCTATAC AAGTCTGAGT 
CCACGTAACC TCCGTAGCCC TGGATCCAAA AGCTCTAAAA AATGTCTGAT TTCAGAGATG 
AGCCAGGAGC CACGTCCTAT TCCTAAAGAT TGTGTTTATC CAGCACTTGT TGGCTGCTCT 
ACTCCAGTAG AAGCTGTTCT GCCACAGATT TCCTCCAACA TGTGGCCGCG TGGCTTCGGC 
CAGCTTGTTC GAGCCAGGAA CATTAAGACA GTAGGAGATC TGAGTGCTCT TACCCCTAAT 
GAAATCAAGT CTCTTCCTAT TCGCTCACCT AAGCTGTCCA ATGTGAGGAA AGCACTTAAA 
ACATATCATG AACAACAGAG AAAAGGACGT TCGGATGACC TGAAGAGTTT TGACGAAATG 
GAGAAAATGA CGTCTGAACC TGAAGAGATG GAGCTTCCTC AAAACCAAGA TGAGGAGCAA 
ACATCAGGAC CAGAGGCTCA AACAGCAGAA TCAGAGACTC AAGACAAAGC CTCAGAAGTG 
GAAGTAAATT CAGAAGTGGT GACTGAAGAG AACCAATCTC ATGATTTGCT CTCAGATATT 
GTAGCTCTTG GTGAACGCTT TACGCCTGAG GAACTCAGTC AGCATTCAGC CAGTCAGTTG 
GGTCTGATGC ATGAACATCT GAGTGGAATG ATGAGGAGCA TTGTGACCAA TCTGCAGTCC 
CGCTTGCTCA GTAATCTGGA CGATAGCTTG CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIF1 ( XM_009304633.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302908.1 Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X2, mRNA.

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CloneID	ODa45048
Clone ID Related Accession (Same CDS sequence)	XM_009304633.2 , XM_009304633.3
Accession Version	XM_009304633.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	telomere-associated protein RIF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304633.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CAGTGCCTTT ATCCAGTACC AGCCTCCTTC CTCTTCTGGA GTGTTTGGAA 
GATGAATCAG CTGGGCTTTC AGCGCAGACG GATGCCTACA TCACCATTGC AAACCGACTT 
AATGGAGAAG AAGGACGCCA GTTTCTGCCT GTGGTTGTTA AGCATTTTGC ACGTCTTAGT 
AAAGTGCTTT TGGTTCACAT CTCCAGTGAG AATGAGGAGC TGTGTCAGGC TGCACTGCAG 
GCTTTGGGAT TCTGTGTCTT CCACTCACAC ATAGTTTCAG TCATACCAGC AAATATTTCA 
GAGAATATAT TGTCTACGCT CTGCAATGTT GTGGTAAAGT CCAAAGAGAA GTTTACGTGC 
ACTCGGGCAT TGTGGGTCAT TTCAAAACAG AATTTCCCTC CAGAGGTGGC ATCCAAAATG 
GCCCCAGAGA TTCTCAAGAG CCTAGAAGCG ATGCAGACCA GAGAGGTGCA GTCCATCCTC 
ATTGACCACG AGTCACTCAA TGTCATCATA AGGTTACTGG AGCAGGCCCC TGTTCCGATG 
GCTGTTGGTG CTGTTTGCTG GGCTAAACTT GTTATTCCTC TGGTGGTTCA TTCGGCCAAT 
AAAGTGCGTC TGCGAGCGGC AGCTGCCCTG GAGCTCGGCA TGCCTCTTCT TCTGGAGAAA 
CAGCAGGAAG TGGCAGCCAT TGTTGAGCCA ATGATGTCTT CTGTGCTCAT CCCTGAAATG 
CAGAAGCTCT TTGCGTCTAA GAACGAGACA AACATGTTGA AACTCTGGCC TTTGTTTGTA 
AGACTTCTGG GCAAGTTACT CCACAAAGGC GGTGCTTTCA TTAACTCCTT GCTGTATCTA 
GAGGAGCTGG GTTTCCGCAA CTCTTCTCCT AATATTAAGA AGATTGCTTT CATAGCTTGG 
AAGAGCCTCA TTGACAATTT TGCCCTTAAT CCAGATATCC TGTGCAGTAG CAAGCGTCTG 
AAGCTCCTCA TGCAGCCGCT CAGCTCCATC CAAGTCAGGA CTGAAGCTCT TCTGCTTACC 
AAGCTGGAGG TCTGGTGGTA TCTTGCTGTC AAGCTCGGAC CCAACCTGTC TGCTAACTTT 
GAACAGGTTG GCGTGCCTCT ATTGCACAGC ACACTTCCTT CTGATTCTCC ACTTCAGAGC 
CCGACTACAC CTGCTCGAAC CTCTAACCCA AGCAACGGCA CACCTAACAC CCCTAAGTCA 
GGCATTCCCT CCTGCAGCAC GCCGAGCACG ACTCCTCGCA TGAATCTGAA CAGCAGCATG 
CAGGCGCCTC AGTCCTATCG TTCCATTCAG CTCCTGGGAC TAGAGATGCT CCTGCACTGG 
CTTGTGGGTC CAGAGGTCAC AGTCACAGCT GCTCGAGAAA ACCTTCAGCT CAGCCTGGAG 
CCCCTGACGC ACCCGCTTTT TACCAGCCCT TCCACCTTCA GCAGACATGC TTCTACCCTC 
ATATCTGCCA GCAAAGAAGG CATCATTACT GTAGGGAAGC ATGCTCCAGA AGCACTTCTT 
AATCTTATTT GGAGCAACCT GATCATTTCT GTCAGTGCAG CAATTGAAGC TGGAAATAAA 
AAGGAGCGAC AGGGTTCAGA GGTCCTCACA CTTTTCCTGC AGGCATTGCA GTCCATTTTG 
TCATCAGACG CTCTGCCTCC TGAACGTGCA CTGCTTCTGT TGGAAGCCAC TGTAAAGGGA 
ATACCTCAGA AGGTGCTTGG TTCTGCAGCT TATCAAGTGG CAAACATGGA CGTGTTAAAC 
GGTACTCCTG CACTTTTCCT AATACTGCTG TTCTATAAAA GTAACCAGCT CTCATCATTT 
CTTGAAGACG AAAGATTTTT TTCAAGCTTG GAGACCCTTG TAAGCTGTGG GTTTTGTGGA 
CCCACATCGC CCTTGGCATT TGGAGAGGCG GTTTTAGGAG CAGTTAATGG GAGTGTGGAG 
ACTGTTAAAA ACAAGAAGCA GCTCTGGAGA ATGTGGAGTA TGGTTGTAAG TCCTCTGACG 
GACACAATTA CTCAGACCAA TGAAGTCAAC CAAGGCGATG CTCTGGAGCA TAACTTCAGT 
GCCATTTACA GTGCGTTGAT GTTCCCCGTC ATCCACCTGC TGCCTTATTC AGCCTTACCA 
CAGACCACTC AGAAGGCCAT GATCAGCACA TGGTCCAGGC TTTACAAGGC TTTTGCCCGT 
TGCTCAGCTT TGGTTGCCAC AGCAGAGGAG AATACATGCT GTGAAGAGTT GTGTGTCAAG 
ATCTCTGCCT CTCTTGACCG TGAAGCTCTA AAAAGTCTGT CTATGCTAGA TGCAATAGCA 
CACATCTTGC TCATCATCGT TGAGAGTATG GACTTTTCTC CATATACCCC TCAGTTCCAG 
CAGAAGATGA AATCTCCTCG TACACCCATG AGTTGGGTAC GCAAGAGAAG CAGAGCCCTC 
GGGAATCTCT CAACCTTCCA CACTCTTCTG ATACAGACTC TAGAGGCTTT TCTCACTGCA 
GACCATTCAG AAGAATCTGC AGAGCCTTTA AATGGAATTG GCTCTGCCTT GGTGTCAATC 
TTGACCTCTC TTTTCACTAA TATTACCCTT CCCTCATTCT TACAAGAGGT GCTCTCCAGC 
CTCACCAAAC CACTTGCACG TCTTTATGAG CAGTTCTCCT GTTGTGATGA CCCATCAAAG 
CTCAGTCCAG AACTAGGGCA CAAGCTGGAG AAACTGGCCG CAGACTTGCT AGGCTGCCTT 
CAGACACATT TGACTCTCCA TGATGATGAA TTCCTCACTG TGTTGTCGCC CTTGCTTTGT 
GTACTTTTTT TGCACAAGAG CAAACAGGTT TGCTCTGTGG TCACTCAATT CTGGAATGCC 
ACTTTTGGAA ATGCGCTCAC CTTGAGTTAC CCAGAACCTC TGAAGTGTGT TTTAAATCAG 
GCTAAGCAGA AGACTCCACT GATTTTACCT GGTTTTGAAG TTGTTGATGT ACCAGATGAT 
TACAGTGGAA CATATATGAG TGAGAGTTCT CAGCTGGAGC CTCAGATTAG CGGGGTAAAA 
GTGACCTCTG TGGGAAAGAG GGACTCTCTT TTGGCAAGAG CAGAAGAGCT AACAGGAAAA 
AGTTCTGTAA CCACATCAAA ACCAGTGTCT GTAAAGCTGG ACTTTGGCTC CCCCAAGCCC 
TTAAGACGAG AAGCTCTTGA GGAGGAAGCA TCTGAAGATT TTGTTTTTAT TCCACCTGAG 
ATGAAAGAGA GAGTCCTGAC TGAGCATCAG AAGGAAGTGA AAAGGACTAA ACGGGTTGAC 
ATTCCTGCCC TGTACAATAA TCTTGATGCC TCCATGGACA CCACAGTCTT TTCACAGTAT 
ACTCAGAGTC AGGAGGAGTC AATGGACACA TTGCCTAAAG GTGAGAAGGA TCATCCTGTG 
GAAGCGGCTA GTGTTCAACT GAGGGAGGTT GCCTCAAATG AAGTACCAAC CACAGATGTT 
AAAGTCCAAA AAGTCACCAG TTCTGATATA GAAACTCCAG ATAAAGCTAC GCCGAATAAA 
GATCAAACTT TGGAGATGGA TCAATCACCA TCTACTGGTG TCTCAGTTGA TGTGAGTGGG 
CTTGAGAAAA GCGCATTGGA GGGGACAAGT CCTAATATTT CAAGTTCATC TGATGTGGTA 
TCTGGCACAC CTCCTAAACC TAACAGCAGG CGTCAGTCAT TTATAACCTT AGAAAAATAT 
GCTGAAGGGA AATCGACAAG TCCTGCAAGT CCTGCATTTA CAGGTACGCT CACCCGGAGA 
TCAAGAAGAC TGGAGGCTTC ACAGGCATCA ACATCAAATC AAAATGATTC TCAACCTCAG 
ATTAAAAAAG TGGATTCTCA GGAATCTCAA AAGATGAGCA GCATTGACAT GTCCATTCAA 
AATGAGCATG CTAGCATGGA AGTAAAAGAT GAGAAGTCAG TGGGAGCTAA TCCATGTGAA 
GGTACAGATG AGGAAGATGT TGTTCCAGAT ACCCAAACCC AAGTATCTGG TGACACATTG 
GGTATTAAAA GCAGTCCTGT GTCTAAAGCC CATGCGGAGG CACAGGGGAC TCCTGAAAAA 
AATTCACAGA GTCCTAATGA TTCTCTAGCT AGTCAAGAAC CAAGAAGGTC TGGCAGAAGG 
CGAAGTAAAC CTGTTCGTCC AGGGCAAGAT GCAGAGGAAA TAAAAAGTAA ATCTCAAGAA 
CAAGCCAGTC AACCTGCAGA CTCTGCGATA ATAAACTCAC AAAAGCCCCT TCTTGCTTCA 
CCTAGTAGTA CTTTGACTGG AAGAAGGAAA AAAAAGGTTG AGACCAATAA AATGGAAGAC 
CAGCTGAACA ATGAAGAAAT GAAAGGAGAT CTCAGCCAAA GCGATTCACA GATTTCCGCT 
GCTGACCCTC CACAGACAGT AGGTGTGCAT GCCAATAAAG ACTTAAGCCA GACTGAGTTA 
TCTCCGAAGA GTTCTCCTGT AACCCAGTCA AATAGCCAAA GCAAAGAGAG ACTGCCAGCT 
CTCTGCGAGT CTGAGAGTAT GTCGTTAGGT AGACAGGAGA CAAAAACACA ACTTGAAGAA 
TCCACAGAAA AGGAGACAAA TAATGACTCT CAAAACGCTT CATCTGCAGC GAGACCGAAG 
CATATACAGT CTACCACGTC TGACTCCGAG TCGGATAGCC AGCAAATTGT CCGTAAACGA 
AGATCGAGGA GATCTGAAAC TAAGTTACTG GAGATATCTG GTGTGCCGAT CAAAGAAGAC 
TTGAATCAGA CAGACTCCCA AACTCCAACA GGAGTGAAGG GAAGAGCTAT GAGGAGGAGA 
ACCGTAGAAG GTGCAGAGTC TAGTAAAGAT TCCACATCAG CGGTAAATAC AGAGGAGTTA 
AGTGCAGGCA AGGAGTCATC ACAAGAGCTT GGTAGATACA GAACTCGACG TTCTAGGGGT 
TTGTTGTCTC CTGAGAGTAT GGAGTCTGAA AACTCTGATA GCCGAGAAGA TGGGGCAAGG 
CCTAAAAAGA GGCTGCGAAA ACCACCATCA ACAGAGGTGT TACCTGTTGC TTCAGAACCC 
AAAGTTATTG AGATGGTACC AGAAAAGGAC GATGTTCAGT CAGAGGTTTT AATGGAGACG 
TCAAAGGATG AGGCAGATCC TGAAAATATC TCGAATACTT CAGTCAGTGA AACCAAAGAA 
CTTGAGCAAA AGAATAAAGA GGAAGTCCCT GCCCCTTGTG ATGCACCACA TGAACAGATT 
ATCACGGAAA AAGCAGCATC AAACAACAAA ATTGACACTG ACTTAACTGA TACAGACGAA 
AAAGAAACCG TCAAGGCAGA GGGTGAAGAA GCTAATGAAT CTTCGCAAAC TGGATCAGAA 
GATTTTAAAA AGACAGATGT TTTGCATAAA TGCCCACATA CAAAGGGACG GGGCCGTGCA 
CGTAGACGAT CTAAAAACTG CAACTGCTTT TCCAACAACC GTGATGTGTT TTCACAGGAC 
AGTGACTTCC AAGAATCACA AGATCTAAAA AATGAACAGG TTCCACTTGC TGCCAACATC 
TTGAACTCTC AACCTGATCA GTCAAGCCCA TCAACTGCAT CAGAAGAAAA CAAAAGCTCA 
GCAATTGAAG AAGTCCCTGT TGCGGATGTT CCATCACTTT CAGCCTTATC AGCTGATCTC 
CCATCTGAGG TTTCACCTTC TGAAAGTCCC ATTACAAAAC TTGGGGTAAT TGTTTCTCAG 
CTACCAGAAG TGGAGAAAGA AGCACAGCCT GGATCATCCA AACCAATGAA GGATTCGAGC 
AAGGAGCTTG TAGAAGAAGT GAATCGATTA AGTTCTGGAT CCACAGGTGG TCAAAAGCAC 
AAAGGAGAAA ATAGATTAGA AAACACTTCA GTAAGCCAAG AACCTGTCCA GAGTTCAACA 
CCTCAAGACA CGTCTCCAAG TCAAGGTCGT CCAGTGGACG ATGCACCAAT ATGCCAGGAG 
CAGTTAGAGT CTTCTCATGT CCAGCAGGAA GATGCAGTTA ACCCAGCAGT GATGGAAAGA 
GAAGACCTTG CTGTGTCAGA GCAGAGACAA GAGAAGGCTG TGTTGTCTGA AGAGTTAGAT 
GGAAAAGGTT TGCTTGACAA GGAAACTACT CTTGTTGAAA AGGAAGAAGA GATGCAGGTT 
AATAACCAGG ATCTTCCTGT GGATGTCCAA GAAGAGTTAC CTGTAGTGTC CCAGAGTAGC 
ACATCTGAAG CCTGCTTGGA TTCTCCACCC AAGCCAAAAC TGTTAAGCGC ACTCAGTGGT 
GAACTAGAGC CTGGCCAGAG TCCAACTAGA AGTCGGTCTC GAGTCTGGTC ACCATCTGCT 
TCTCCGTCTA CCAGTATTCT GAAGAAGGGT CAGAAGAGAG CATGTGAAGA GGAGACTCCA 
TCCCCCATTC CCAAGTCACG TCGAGTGTCT TTTGCAACTC CAATTTACCA GCAAGAACTG 
GCTGATGACA TTGATCGTCG TAGTCCGGTT ATTCGCACAA GTTCACCTAG GTCAAAAGTC 
TTGAGTGGAC AGTCAAAGTA TATCACTACA CCAACAAAAG GTGGCTATAC AAGTCTGAGT 
CCACGTAACC TCCGTAGCCC TGGATCCAAA AGCTCTAAAA AATGTCTGAT TTCAGAGATG 
AGCCAGGAGC CACGTCCTAT TCCTAAAGAT TGTGTTTATC CAGCACTTGT TGGCTGCTCT 
ACTCCAGTAG AAGCTGTTCT GCCACAGATT TCCTCCAACA TGTGGCCGCG TGGCTTCGGC 
CAGCTTGTTC GAGCCAGGAA CATTAAGACA GTAGGAGATC TGAGTGCTCT TACCCCTAAT 
GAAATCAAGT CTCTTCCTAT TCGCTCACCT AAGCTGTCCA ATGTGAGGAA AGCACTTAAA 
ACATATCATG AACAACAGAG AAAAGGACGT TCGGATGACC TGAAGAGTTT TGACGAAATG 
GAGAAAATGA CGTCTGAACC TGAAGAGATG GAGCTTCCTC AAAACCAAGA TGAGGAGCAA 
ACATCAGGAC CAGAGGCTCA AACAGCAGAA TCAGAGACTC AAGACAAAGC CTCAGAAGTG 
GAAGTAAATT CAGAAGTGGT GACTGAAGAG AACCAATCTC ATGATTTGCT CTCAGATATT 
GTAGCTCTTG GTGAACGCTT TACGCCTGAG GAACTCAGTC AGCATTCAGC CAGTCAGTTG 
GGTCTGATGC ATGAACATCT GAGTGGAATG ATGAGGAGCA TTGTGACCAA TCTGCAGTCC 
CGCTTGCTCA GTAATCTGGA CGATAGCTTG CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302908.1
CDS	192..7367
Translation	MMAAVPLSSTSLLPLLECLEDESAGLSAQTDAYITIANRLNGEEGRQFLPVVVKHFARLSKVLLVHISSENEELCQAALQALGFCVFHSHIVSVIPANISENILSTLCNVVVKSKEKFTCTRALWVISKQNFPPEVASKMAPEILKSLEAMQTREVQSILIDHESLNVIIRLLEQAPVPMAVGAVCWAKLVIPLVVHSANKVRLRAAAALELGMPLLLEKQQEVAAIVEPMMSSVLIPEMQKLFASKNETNMLKLWPLFVRLLGKLLHKGGAFINSLLYLEELGFRNSSPNIKKIAFIAWKSLIDNFALNPDILCSSKRLKLLMQPLSSIQVRTEALLLTKLEVWWYLAVKLGPNLSANFEQVGVPLLHSTLPSDSPLQSPTTPARTSNPSNGTPNTPKSGIPSCSTPSTTPRMNLNSSMQAPQSYRSIQLLGLEMLLHWLVGPEVTVTAARENLQLSLEPLTHPLFTSPSTFSRHASTLISASKEGIITVGKHAPEALLNLIWSNLIISVSAAIEAGNKKERQGSEVLTLFLQALQSILSSDALPPERALLLLEATVKGIPQKVLGSAAYQVANMDVLNGTPALFLILLFYKSNQLSSFLEDERFFSSLETLVSCGFCGPTSPLAFGEAVLGAVNGSVETVKNKKQLWRMWSMVVSPLTDTITQTNEVNQGDALEHNFSAIYSALMFPVIHLLPYSALPQTTQKAMISTWSRLYKAFARCSALVATAEENTCCEELCVKISASLDREALKSLSMLDAIAHILLIIVESMDFSPYTPQFQQKMKSPRTPMSWVRKRSRALGNLSTFHTLLIQTLEAFLTADHSEESAEPLNGIGSALVSILTSLFTNITLPSFLQEVLSSLTKPLARLYEQFSCCDDPSKLSPELGHKLEKLAADLLGCLQTHLTLHDDEFLTVLSPLLCVLFLHKSKQVCSVVTQFWNATFGNALTLSYPEPLKCVLNQAKQKTPLILPGFEVVDVPDDYSGTYMSESSQLEPQISGVKVTSVGKRDSLLARAEELTGKSSVTTSKPVSVKLDFGSPKPLRREALEEEASEDFVFIPPEMKERVLTEHQKEVKRTKRVDIPALYNNLDASMDTTVFSQYTQSQEESMDTLPKGEKDHPVEAASVQLREVASNEVPTTDVKVQKVTSSDIETPDKATPNKDQTLEMDQSPSTGVSVDVSGLEKSALEGTSPNISSSSDVVSGTPPKPNSRRQSFITLEKYAEGKSTSPASPAFTGTLTRRSRRLEASQASTSNQNDSQPQIKKVDSQESQKMSSIDMSIQNEHASMEVKDEKSVGANPCEGTDEEDVVPDTQTQVSGDTLGIKSSPVSKAHAEAQGTPEKNSQSPNDSLASQEPRRSGRRRSKPVRPGQDAEEIKSKSQEQASQPADSAIINSQKPLLASPSSTLTGRRKKKVETNKMEDQLNNEEMKGDLSQSDSQISAADPPQTVGVHANKDLSQTELSPKSSPVTQSNSQSKERLPALCESESMSLGRQETKTQLEESTEKETNNDSQNASSAARPKHIQSTTSDSESDSQQIVRKRRSRRSETKLLEISGVPIKEDLNQTDSQTPTGVKGRAMRRRTVEGAESSKDSTSAVNTEELSAGKESSQELGRYRTRRSRGLLSPESMESENSDSREDGARPKKRLRKPPSTEVLPVASEPKVIEMVPEKDDVQSEVLMETSKDEADPENISNTSVSETKELEQKNKEEVPAPCDAPHEQIITEKAASNNKIDTDLTDTDEKETVKAEGEEANESSQTGSEDFKKTDVLHKCPHTKGRGRARRRSKNCNCFSNNRDVFSQDSDFQESQDLKNEQVPLAANILNSQPDQSSPSTASEENKSSAIEEVPVADVPSLSALSADLPSEVSPSESPITKLGVIVSQLPEVEKEAQPGSSKPMKDSSKELVEEVNRLSSGSTGGQKHKGENRLENTSVSQEPVQSSTPQDTSPSQGRPVDDAPICQEQLESSHVQQEDAVNPAVMEREDLAVSEQRQEKAVLSEELDGKGLLDKETTLVEKEEEMQVNNQDLPVDVQEELPVVSQSSTSEACLDSPPKPKLLSALSGELEPGQSPTRSRSRVWSPSASPSTSILKKGQKRACEEETPSPIPKSRRVSFATPIYQQELADDIDRRSPVIRTSSPRSKVLSGQSKYITTPTKGGYTSLSPRNLRSPGSKSSKKCLISEMSQEPRPIPKDCVYPALVGCSTPVEAVLPQISSNMWPRGFGQLVRARNIKTVGDLSALTPNEIKSLPIRSPKLSNVRKALKTYHEQQRKGRSDDLKSFDEMEKMTSEPEEMELPQNQDEEQTSGPEAQTAESETQDKASEVEVNSEVVTEENQSHDLLSDIVALGERFTPEELSQHSASQLGLMHEHLSGMMRSIVTNLQSRLLSNLDDSLP

Target ORF information:

RefSeq Version	XM_009304633.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio replication timing regulatory factor 1 (rif1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304633.3

ORF Insert Sequence:

ATGATGGCGG CAGTGCCTTT ATCCAGTACC AGCCTCCTTC CTCTTCTGGA GTGTTTGGAA 
GATGAATCAG CTGGGCTTTC AGCGCAGACG GATGCCTACA TCACCATTGC AAACCGACTT 
AATGGAGAAG AAGGACGCCA GTTTCTGCCT GTGGTTGTTA AGCATTTTGC ACGTCTTAGT 
AAAGTGCTTT TGGTTCACAT CTCCAGTGAG AATGAGGAGC TGTGTCAGGC TGCACTGCAG 
GCTTTGGGAT TCTGTGTCTT CCACTCACAC ATAGTTTCAG TCATACCAGC AAATATTTCA 
GAGAATATAT TGTCTACGCT CTGCAATGTT GTGGTAAAGT CCAAAGAGAA GTTTACGTGC 
ACTCGGGCAT TGTGGGTCAT TTCAAAACAG AATTTCCCTC CAGAGGTGGC ATCCAAAATG 
GCCCCAGAGA TTCTCAAGAG CCTAGAAGCG ATGCAGACCA GAGAGGTGCA GTCCATCCTC 
ATTGACCACG AGTCACTCAA TGTCATCATA AGGTTACTGG AGCAGGCCCC TGTTCCGATG 
GCTGTTGGTG CTGTTTGCTG GGCTAAACTT GTTATTCCTC TGGTGGTTCA TTCGGCCAAT 
AAAGTGCGTC TGCGAGCGGC AGCTGCCCTG GAGCTCGGCA TGCCTCTTCT TCTGGAGAAA 
CAGCAGGAAG TGGCAGCCAT TGTTGAGCCA ATGATGTCTT CTGTGCTCAT CCCTGAAATG 
CAGAAGCTCT TTGCGTCTAA GAACGAGACA AACATGTTGA AACTCTGGCC TTTGTTTGTA 
AGACTTCTGG GCAAGTTACT CCACAAAGGC GGTGCTTTCA TTAACTCCTT GCTGTATCTA 
GAGGAGCTGG GTTTCCGCAA CTCTTCTCCT AATATTAAGA AGATTGCTTT CATAGCTTGG 
AAGAGCCTCA TTGACAATTT TGCCCTTAAT CCAGATATCC TGTGCAGTAG CAAGCGTCTG 
AAGCTCCTCA TGCAGCCGCT CAGCTCCATC CAAGTCAGGA CTGAAGCTCT TCTGCTTACC 
AAGCTGGAGG TCTGGTGGTA TCTTGCTGTC AAGCTCGGAC CCAACCTGTC TGCTAACTTT 
GAACAGGTTG GCGTGCCTCT ATTGCACAGC ACACTTCCTT CTGATTCTCC ACTTCAGAGC 
CCGACTACAC CTGCTCGAAC CTCTAACCCA AGCAACGGCA CACCTAACAC CCCTAAGTCA 
GGCATTCCCT CCTGCAGCAC GCCGAGCACG ACTCCTCGCA TGAATCTGAA CAGCAGCATG 
CAGGCGCCTC AGTCCTATCG TTCCATTCAG CTCCTGGGAC TAGAGATGCT CCTGCACTGG 
CTTGTGGGTC CAGAGGTCAC AGTCACAGCT GCTCGAGAAA ACCTTCAGCT CAGCCTGGAG 
CCCCTGACGC ACCCGCTTTT TACCAGCCCT TCCACCTTCA GCAGACATGC TTCTACCCTC 
ATATCTGCCA GCAAAGAAGG CATCATTACT GTAGGGAAGC ATGCTCCAGA AGCACTTCTT 
AATCTTATTT GGAGCAACCT GATCATTTCT GTCAGTGCAG CAATTGAAGC TGGAAATAAA 
AAGGAGCGAC AGGGTTCAGA GGTCCTCACA CTTTTCCTGC AGGCATTGCA GTCCATTTTG 
TCATCAGACG CTCTGCCTCC TGAACGTGCA CTGCTTCTGT TGGAAGCCAC TGTAAAGGGA 
ATACCTCAGA AGGTGCTTGG TTCTGCAGCT TATCAAGTGG CAAACATGGA CGTGTTAAAC 
GGTACTCCTG CACTTTTCCT AATACTGCTG TTCTATAAAA GTAACCAGCT CTCATCATTT 
CTTGAAGACG AAAGATTTTT TTCAAGCTTG GAGACCCTTG TAAGCTGTGG GTTTTGTGGA 
CCCACATCGC CCTTGGCATT TGGAGAGGCG GTTTTAGGAG CAGTTAATGG GAGTGTGGAG 
ACTGTTAAAA ACAAGAAGCA GCTCTGGAGA ATGTGGAGTA TGGTTGTAAG TCCTCTGACG 
GACACAATTA CTCAGACCAA TGAAGTCAAC CAAGGCGATG CTCTGGAGCA TAACTTCAGT 
GCCATTTACA GTGCGTTGAT GTTCCCCGTC ATCCACCTGC TGCCTTATTC AGCCTTACCA 
CAGACCACTC AGAAGGCCAT GATCAGCACA TGGTCCAGGC TTTACAAGGC TTTTGCCCGT 
TGCTCAGCTT TGGTTGCCAC AGCAGAGGAG AATACATGCT GTGAAGAGTT GTGTGTCAAG 
ATCTCTGCCT CTCTTGACCG TGAAGCTCTA AAAAGTCTGT CTATGCTAGA TGCAATAGCA 
CACATCTTGC TCATCATCGT TGAGAGTATG GACTTTTCTC CATATACCCC TCAGTTCCAG 
CAGAAGATGA AATCTCCTCG TACACCCATG AGTTGGGTAC GCAAGAGAAG CAGAGCCCTC 
GGGAATCTCT CAACCTTCCA CACTCTTCTG ATACAGACTC TAGAGGCTTT TCTCACTGCA 
GACCATTCAG AAGAATCTGC AGAGCCTTTA AATGGAATTG GCTCTGCCTT GGTGTCAATC 
TTGACCTCTC TTTTCACTAA TATTACCCTT CCCTCATTCT TACAAGAGGT GCTCTCCAGC 
CTCACCAAAC CACTTGCACG TCTTTATGAG CAGTTCTCCT GTTGTGATGA CCCATCAAAG 
CTCAGTCCAG AACTAGGGCA CAAGCTGGAG AAACTGGCCG CAGACTTGCT AGGCTGCCTT 
CAGACACATT TGACTCTCCA TGATGATGAA TTCCTCACTG TGTTGTCGCC CTTGCTTTGT 
GTACTTTTTT TGCACAAGAG CAAACAGGTT TGCTCTGTGG TCACTCAATT CTGGAATGCC 
ACTTTTGGAA ATGCGCTCAC CTTGAGTTAC CCAGAACCTC TGAAGTGTGT TTTAAATCAG 
GCTAAGCAGA AGACTCCACT GATTTTACCT GGTTTTGAAG TTGTTGATGT ACCAGATGAT 
TACAGTGGAA CATATATGAG TGAGAGTTCT CAGCTGGAGC CTCAGATTAG CGGGGTAAAA 
GTGACCTCTG TGGGAAAGAG GGACTCTCTT TTGGCAAGAG CAGAAGAGCT AACAGGAAAA 
AGTTCTGTAA CCACATCAAA ACCAGTGTCT GTAAAGCTGG ACTTTGGCTC CCCCAAGCCC 
TTAAGACGAG AAGCTCTTGA GGAGGAAGCA TCTGAAGATT TTGTTTTTAT TCCACCTGAG 
ATGAAAGAGA GAGTCCTGAC TGAGCATCAG AAGGAAGTGA AAAGGACTAA ACGGGTTGAC 
ATTCCTGCCC TGTACAATAA TCTTGATGCC TCCATGGACA CCACAGTCTT TTCACAGTAT 
ACTCAGAGTC AGGAGGAGTC AATGGACACA TTGCCTAAAG GTGAGAAGGA TCATCCTGTG 
GAAGCGGCTA GTGTTCAACT GAGGGAGGTT GCCTCAAATG AAGTACCAAC CACAGATGTT 
AAAGTCCAAA AAGTCACCAG TTCTGATATA GAAACTCCAG ATAAAGCTAC GCCGAATAAA 
GATCAAACTT TGGAGATGGA TCAATCACCA TCTACTGGTG TCTCAGTTGA TGTGAGTGGG 
CTTGAGAAAA GCGCATTGGA GGGGACAAGT CCTAATATTT CAAGTTCATC TGATGTGGTA 
TCTGGCACAC CTCCTAAACC TAACAGCAGG CGTCAGTCAT TTATAACCTT AGAAAAATAT 
GCTGAAGGGA AATCGACAAG TCCTGCAAGT CCTGCATTTA CAGGTACGCT CACCCGGAGA 
TCAAGAAGAC TGGAGGCTTC ACAGGCATCA ACATCAAATC AAAATGATTC TCAACCTCAG 
ATTAAAAAAG TGGATTCTCA GGAATCTCAA AAGATGAGCA GCATTGACAT GTCCATTCAA 
AATGAGCATG CTAGCATGGA AGTAAAAGAT GAGAAGTCAG TGGGAGCTAA TCCATGTGAA 
GGTACAGATG AGGAAGATGT TGTTCCAGAT ACCCAAACCC AAGTATCTGG TGACACATTG 
GGTATTAAAA GCAGTCCTGT GTCTAAAGCC CATGCGGAGG CACAGGGGAC TCCTGAAAAA 
AATTCACAGA GTCCTAATGA TTCTCTAGCT AGTCAAGAAC CAAGAAGGTC TGGCAGAAGG 
CGAAGTAAAC CTGTTCGTCC AGGGCAAGAT GCAGAGGAAA TAAAAAGTAA ATCTCAAGAA 
CAAGCCAGTC AACCTGCAGA CTCTGCGATA ATAAACTCAC AAAAGCCCCT TCTTGCTTCA 
CCTAGTAGTA CTTTGACTGG AAGAAGGAAA AAAAAGGTTG AGACCAATAA AATGGAAGAC 
CAGCTGAACA ATGAAGAAAT GAAAGGAGAT CTCAGCCAAA GCGATTCACA GATTTCCGCT 
GCTGACCCTC CACAGACAGT AGGTGTGCAT GCCAATAAAG ACTTAAGCCA GACTGAGTTA 
TCTCCGAAGA GTTCTCCTGT AACCCAGTCA AATAGCCAAA GCAAAGAGAG ACTGCCAGCT 
CTCTGCGAGT CTGAGAGTAT GTCGTTAGGT AGACAGGAGA CAAAAACACA ACTTGAAGAA 
TCCACAGAAA AGGAGACAAA TAATGACTCT CAAAACGCTT CATCTGCAGC GAGACCGAAG 
CATATACAGT CTACCACGTC TGACTCCGAG TCGGATAGCC AGCAAATTGT CCGTAAACGA 
AGATCGAGGA GATCTGAAAC TAAGTTACTG GAGATATCTG GTGTGCCGAT CAAAGAAGAC 
TTGAATCAGA CAGACTCCCA AACTCCAACA GGAGTGAAGG GAAGAGCTAT GAGGAGGAGA 
ACCGTAGAAG GTGCAGAGTC TAGTAAAGAT TCCACATCAG CGGTAAATAC AGAGGAGTTA 
AGTGCAGGCA AGGAGTCATC ACAAGAGCTT GGTAGATACA GAACTCGACG TTCTAGGGGT 
TTGTTGTCTC CTGAGAGTAT GGAGTCTGAA AACTCTGATA GCCGAGAAGA TGGGGCAAGG 
CCTAAAAAGA GGCTGCGAAA ACCACCATCA ACAGAGGTGT TACCTGTTGC TTCAGAACCC 
AAAGTTATTG AGATGGTACC AGAAAAGGAC GATGTTCAGT CAGAGGTTTT AATGGAGACG 
TCAAAGGATG AGGCAGATCC TGAAAATATC TCGAATACTT CAGTCAGTGA AACCAAAGAA 
CTTGAGCAAA AGAATAAAGA GGAAGTCCCT GCCCCTTGTG ATGCACCACA TGAACAGATT 
ATCACGGAAA AAGCAGCATC AAACAACAAA ATTGACACTG ACTTAACTGA TACAGACGAA 
AAAGAAACCG TCAAGGCAGA GGGTGAAGAA GCTAATGAAT CTTCGCAAAC TGGATCAGAA 
GATTTTAAAA AGACAGATGT TTTGCATAAA TGCCCACATA CAAAGGGACG GGGCCGTGCA 
CGTAGACGAT CTAAAAACTG CAACTGCTTT TCCAACAACC GTGATGTGTT TTCACAGGAC 
AGTGACTTCC AAGAATCACA AGATCTAAAA AATGAACAGG TTCCACTTGC TGCCAACATC 
TTGAACTCTC AACCTGATCA GTCAAGCCCA TCAACTGCAT CAGAAGAAAA CAAAAGCTCA 
GCAATTGAAG AAGTCCCTGT TGCGGATGTT CCATCACTTT CAGCCTTATC AGCTGATCTC 
CCATCTGAGG TTTCACCTTC TGAAAGTCCC ATTACAAAAC TTGGGGTAAT TGTTTCTCAG 
CTACCAGAAG TGGAGAAAGA AGCACAGCCT GGATCATCCA AACCAATGAA GGATTCGAGC 
AAGGAGCTTG TAGAAGAAGT GAATCGATTA AGTTCTGGAT CCACAGGTGG TCAAAAGCAC 
AAAGGAGAAA ATAGATTAGA AAACACTTCA GTAAGCCAAG AACCTGTCCA GAGTTCAACA 
CCTCAAGACA CGTCTCCAAG TCAAGGTCGT CCAGTGGACG ATGCACCAAT ATGCCAGGAG 
CAGTTAGAGT CTTCTCATGT CCAGCAGGAA GATGCAGTTA ACCCAGCAGT GATGGAAAGA 
GAAGACCTTG CTGTGTCAGA GCAGAGACAA GAGAAGGCTG TGTTGTCTGA AGAGTTAGAT 
GGAAAAGGTT TGCTTGACAA GGAAACTACT CTTGTTGAAA AGGAAGAAGA GATGCAGGTT 
AATAACCAGG ATCTTCCTGT GGATGTCCAA GAAGAGTTAC CTGTAGTGTC CCAGAGTAGC 
ACATCTGAAG CCTGCTTGGA TTCTCCACCC AAGCCAAAAC TGTTAAGCGC ACTCAGTGGT 
GAACTAGAGC CTGGCCAGAG TCCAACTAGA AGTCGGTCTC GAGTCTGGTC ACCATCTGCT 
TCTCCGTCTA CCAGTATTCT GAAGAAGGGT CAGAAGAGAG CATGTGAAGA GGAGACTCCA 
TCCCCCATTC CCAAGTCACG TCGAGTGTCT TTTGCAACTC CAATTTACCA GCAAGAACTG 
GCTGATGACA TTGATCGTCG TAGTCCGGTT ATTCGCACAA GTTCACCTAG GTCAAAAGTC 
TTGAGTGGAC AGTCAAAGTA TATCACTACA CCAACAAAAG GTGGCTATAC AAGTCTGAGT 
CCACGTAACC TCCGTAGCCC TGGATCCAAA AGCTCTAAAA AATGTCTGAT TTCAGAGATG 
AGCCAGGAGC CACGTCCTAT TCCTAAAGAT TGTGTTTATC CAGCACTTGT TGGCTGCTCT 
ACTCCAGTAG AAGCTGTTCT GCCACAGATT TCCTCCAACA TGTGGCCGCG TGGCTTCGGC 
CAGCTTGTTC GAGCCAGGAA CATTAAGACA GTAGGAGATC TGAGTGCTCT TACCCCTAAT 
GAAATCAAGT CTCTTCCTAT TCGCTCACCT AAGCTGTCCA ATGTGAGGAA AGCACTTAAA 
ACATATCATG AACAACAGAG AAAAGGACGT TCGGATGACC TGAAGAGTTT TGACGAAATG 
GAGAAAATGA CGTCTGAACC TGAAGAGATG GAGCTTCCTC AAAACCAAGA TGAGGAGCAA 
ACATCAGGAC CAGAGGCTCA AACAGCAGAA TCAGAGACTC AAGACAAAGC CTCAGAAGTG 
GAAGTAAATT CAGAAGTGGT GACTGAAGAG AACCAATCTC ATGATTTGCT CTCAGATATT 
GTAGCTCTTG GTGAACGCTT TACGCCTGAG GAACTCAGTC AGCATTCAGC CAGTCAGTTG 
GGTCTGATGC ATGAACATCT GAGTGGAATG ATGAGGAGCA TTGTGACCAA TCTGCAGTCC 
CGCTTGCTCA GTAATCTGGA CGATAGCTTG CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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RIF1 cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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