fyco1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	fyco1a
Entrez Gene ID	565649
Full Name	FYVE and coiled-coil domain containing 1a
Synonyms	si:ch73-217b7.5
General protein information	Preferred Names FYVE and coiled-coil domain containing 1a Names FYVE and coiled-coil domain-containing protein 1-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017353733.2	XP_017209222.1	FYVE and coiled-coil domain-containing protein 1-like isoform X2
XM_017353733.1	XP_017209222.1	FYVE and coiled-coil domain-containing protein 1-like isoform X2
XM_009297090.2	XP_009295365.1	FYVE and coiled-coil domain-containing protein 1-like isoform X1
XM_009297091.2	XP_009295366.1	FYVE and coiled-coil domain-containing protein 1-like isoform X1
XM_009297090.3	XP_009295365.1	FYVE and coiled-coil domain-containing protein 1-like isoform X1
XM_009297091.3	XP_009295366.1	FYVE and coiled-coil domain-containing protein 1-like isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC565649	XP_003201276.2
Canis lupus familiaris (dog)	FYCO1	XP_005632690.1
Gallus gallus (chicken)	FYCO1	NP_001039304.2
Bos taurus (cattle)	FYCO1	XP_005223169.1
Pan troglodytes (chimpanzee)	FYCO1	XP_003309815.1
Homo sapiens (human)	FYCO1	NP_078789.2
Macaca mulatta (Rhesus monkey)	FYCO1	XP_001114357.1
Mus musculus (house mouse)	Fyco1	NP_683727.3
Rattus norvegicus (Norway rat)	Fyco1	NP_001100340.1

Gene Ontology

The following fyco1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fyco1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa44696	XM_009297091.3 Latest version!	Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa44696	XM_009297090.3 Latest version!	Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa57511	XM_017353733.2 Latest version!	Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa57511	XM_017353733.1	Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa44696	XM_009297090.2	Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa44696	XM_009297091.2	Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fyco1a ( XM_009297090.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295365.1 Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44696
Clone ID Related Accession (Same CDS sequence)	XM_009297090.2 , XM_009297091.2 , XM_009297090.3 , XM_009297091.3
Accession Version	XM_009297090.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	FYVE and coiled-coil domain-containing protein 1-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297090.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC 
AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG 
GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG 
GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG 
ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA 
GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG 
AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT 
GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT 
GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT 
AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG 
AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG 
AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA 
AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT 
GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT 
CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG 
GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC 
ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG 
GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT 
TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG 
AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA 
GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG 
GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG 
ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG 
CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG 
AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT 
CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC 
GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT 
GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG 
GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC 
GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA 
GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT 
CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG 
GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG 
AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG 
AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT 
CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG 
GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT 
CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT 
CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA 
TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT 
CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC 
TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG 
CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG 
AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT 
CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG 
AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC 
GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT 
CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA 
GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG 
GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC 
ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT 
CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA 
GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT 
GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG 
ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG 
GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT 
CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG 
CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC 
GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT 
CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC 
CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG 
CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT 
GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT 
CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA 
GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC 
ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC 
GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC 
AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC 
GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG 
GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC 
ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC 
CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT 
GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC 
TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295365.1
CDS	134..4627
Translation	MATGAAVGDSQIQRIIRDLRDAVSELTKEYKESGEPITDDSSNLHKFSYKLEYLLQFDQKEKTTFLGSRKDYWDYFSDCLAKIKGANDGIRFVKSISELKTSLGKGRAFIRYSLVHQRLADTLQQCLMNSRVTSDWYNPRSPFLKPHLSVDIISYLYELNDVQFDVASRGHDLDAEWPTFARRTLGMTSSPSHLWKAPSRSSSINSLASTYSQQHHEFPGSPDFGPGLLSDMSMQNSSILNDTSMSAIDELRLELDQSELKQRELIDRIQQLGDEGSELRGVVVELQRQLDVSLAAQGNHQELQRNLEVLIESEHALSREVEVLRDRETRREVSHKDLQDMLAAAERKNEELMTRLDGVLDEKGQRAASDFNSAQKIHELLNELKEAEKKRMDALAEGEEKRRHAEHLAEEVKVKDEALKEAEVKMAAWMEKGEQLQTRAVEQRNFMEKLQGALAVREKETSNLQRQLRDLQNSLENMEKQANVEKKRMQDDKEELEMKMNGLEGLLQSLRTQLKVKESDLLSSTKRVHFLERESEKLRSENQKLEYELENSTKKEAKKIDEYKDSCAKLIEQNTKLLQTVNKNEESKKELLENKSSLESELAGLRASEKQLRAQIDDAKVTVDEREQRLREENRNLDESLQKANMQLEESESSIRQKEQENKDLMEVQVTLKSALAAMQKEIRDINNQIGELEKNLGVARCNEANLNAQLKDKATQLEDREKLCEELQGRVEELESRQRDLEVEKTKAERAFVKQTEMIQSLEAQRNLAEKTQLEKSTCQAKETKEMALKLTLLEDQLGLSAKEVSKLQEEVVNLRAKLHSAVEEKDKTQAKLEVTEASCAELRILTEHLKKQAEEQNRLHVSELLQSSEHVDKLTSQLNQETSAHEKTTAALASAKEDLVALKAQNERMVLENAETRESLHRVNTEMAELGMTICKLTAEREEARERWAAEAVRIQELQQHGVKETERLNASLVALHQENSSLQEELQQTDKLSETMLELKQLLDKTEGERDAAREEITAVKFQMSTESMSLKHQMKSLQEEIDGLKDQLDTERKKKSELEAKLSELEGANVEYSRLIEEKDSHITYCETLLRESESETQQLQERASRSKEALSDVEKEREELKQKLDQVLMETQNQHLRMSAELEDLGQTKVNLEERLIELIRDKDALWQKSDALEFEQKLRAEEQWWLVDKEATHCLGCQGQFTWWLRRHHCRLCGRIFCYYCSNNYVMTKNSKKERCCRECYTQHGAVVERFTKAELNSSSDNQPSNTAAQLPPPPYIPTPRVTVTDPGERADDGAFDIITDEEVNQIYDSDSRTTNGSHEEEGGQTGDNSVSSDVTSEEQEDMISVQDAEITLLKSGELSASVPLDIDEIPHFGDGSRELFVKSSCYSTIPITVGDRGPIISWVFSSEPKSISFTVVYRETLDTPVEQAKVLIPLTRCNSHKETIQGQLKVRNAGVYTLIFDNSFSRFLSKKVNYHLTIEKPVIYDGSDFP

Target ORF information:

RefSeq Version	XM_009297090.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297090.2

ORF Insert Sequence:

ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC 
AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG 
GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG 
GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG 
ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA 
GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG 
AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT 
GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT 
GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT 
AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG 
AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG 
AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA 
AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT 
GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT 
CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG 
GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC 
ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG 
GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT 
TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG 
AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA 
GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG 
GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG 
ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG 
CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG 
AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT 
CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC 
GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT 
GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG 
GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC 
GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA 
GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT 
CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG 
GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG 
AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG 
AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT 
CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG 
GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT 
CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT 
CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA 
TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT 
CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC 
TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG 
CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG 
AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT 
CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG 
AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC 
GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT 
CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA 
GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG 
GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC 
ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT 
CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA 
GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT 
GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG 
ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG 
GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT 
CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG 
CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC 
GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT 
CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC 
CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG 
CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT 
GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT 
CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA 
GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC 
ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC 
GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC 
AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC 
GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG 
GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC 
ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC 
CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT 
GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC 
TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fyco1a ( XM_009297091.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295366.1 Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44696
Clone ID Related Accession (Same CDS sequence)	XM_009297090.2 , XM_009297091.2 , XM_009297090.3 , XM_009297091.3
Accession Version	XM_009297091.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	FYVE and coiled-coil domain-containing protein 1-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297091.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC 
AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG 
GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG 
GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG 
ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA 
GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG 
AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT 
GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT 
GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT 
AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG 
AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG 
AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA 
AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT 
GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT 
CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG 
GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC 
ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG 
GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT 
TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG 
AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA 
GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG 
GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG 
ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG 
CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG 
AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT 
CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC 
GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT 
GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG 
GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC 
GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA 
GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT 
CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG 
GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG 
AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG 
AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT 
CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG 
GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT 
CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT 
CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA 
TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT 
CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC 
TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG 
CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG 
AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT 
CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG 
AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC 
GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT 
CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA 
GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG 
GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC 
ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT 
CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA 
GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT 
GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG 
ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG 
GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT 
CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG 
CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC 
GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT 
CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC 
CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG 
CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT 
GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT 
CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA 
GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC 
ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC 
GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC 
AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC 
GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG 
GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC 
ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC 
CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT 
GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC 
TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295366.1
CDS	88..4581
Translation	MATGAAVGDSQIQRIIRDLRDAVSELTKEYKESGEPITDDSSNLHKFSYKLEYLLQFDQKEKTTFLGSRKDYWDYFSDCLAKIKGANDGIRFVKSISELKTSLGKGRAFIRYSLVHQRLADTLQQCLMNSRVTSDWYNPRSPFLKPHLSVDIISYLYELNDVQFDVASRGHDLDAEWPTFARRTLGMTSSPSHLWKAPSRSSSINSLASTYSQQHHEFPGSPDFGPGLLSDMSMQNSSILNDTSMSAIDELRLELDQSELKQRELIDRIQQLGDEGSELRGVVVELQRQLDVSLAAQGNHQELQRNLEVLIESEHALSREVEVLRDRETRREVSHKDLQDMLAAAERKNEELMTRLDGVLDEKGQRAASDFNSAQKIHELLNELKEAEKKRMDALAEGEEKRRHAEHLAEEVKVKDEALKEAEVKMAAWMEKGEQLQTRAVEQRNFMEKLQGALAVREKETSNLQRQLRDLQNSLENMEKQANVEKKRMQDDKEELEMKMNGLEGLLQSLRTQLKVKESDLLSSTKRVHFLERESEKLRSENQKLEYELENSTKKEAKKIDEYKDSCAKLIEQNTKLLQTVNKNEESKKELLENKSSLESELAGLRASEKQLRAQIDDAKVTVDEREQRLREENRNLDESLQKANMQLEESESSIRQKEQENKDLMEVQVTLKSALAAMQKEIRDINNQIGELEKNLGVARCNEANLNAQLKDKATQLEDREKLCEELQGRVEELESRQRDLEVEKTKAERAFVKQTEMIQSLEAQRNLAEKTQLEKSTCQAKETKEMALKLTLLEDQLGLSAKEVSKLQEEVVNLRAKLHSAVEEKDKTQAKLEVTEASCAELRILTEHLKKQAEEQNRLHVSELLQSSEHVDKLTSQLNQETSAHEKTTAALASAKEDLVALKAQNERMVLENAETRESLHRVNTEMAELGMTICKLTAEREEARERWAAEAVRIQELQQHGVKETERLNASLVALHQENSSLQEELQQTDKLSETMLELKQLLDKTEGERDAAREEITAVKFQMSTESMSLKHQMKSLQEEIDGLKDQLDTERKKKSELEAKLSELEGANVEYSRLIEEKDSHITYCETLLRESESETQQLQERASRSKEALSDVEKEREELKQKLDQVLMETQNQHLRMSAELEDLGQTKVNLEERLIELIRDKDALWQKSDALEFEQKLRAEEQWWLVDKEATHCLGCQGQFTWWLRRHHCRLCGRIFCYYCSNNYVMTKNSKKERCCRECYTQHGAVVERFTKAELNSSSDNQPSNTAAQLPPPPYIPTPRVTVTDPGERADDGAFDIITDEEVNQIYDSDSRTTNGSHEEEGGQTGDNSVSSDVTSEEQEDMISVQDAEITLLKSGELSASVPLDIDEIPHFGDGSRELFVKSSCYSTIPITVGDRGPIISWVFSSEPKSISFTVVYRETLDTPVEQAKVLIPLTRCNSHKETIQGQLKVRNAGVYTLIFDNSFSRFLSKKVNYHLTIEKPVIYDGSDFP

Target ORF information:

RefSeq Version	XM_009297091.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297091.2

ORF Insert Sequence:

ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC 
AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG 
GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG 
GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG 
ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA 
GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG 
AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT 
GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT 
GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT 
AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG 
AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG 
AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA 
AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT 
GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT 
CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG 
GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC 
ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG 
GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT 
TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG 
AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA 
GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG 
GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG 
ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG 
CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG 
AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT 
CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC 
GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT 
GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG 
GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC 
GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA 
GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT 
CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG 
GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG 
AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG 
AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT 
CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG 
GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT 
CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT 
CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA 
TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT 
CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC 
TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG 
CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG 
AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT 
CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG 
AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC 
GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT 
CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA 
GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG 
GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC 
ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT 
CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA 
GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT 
GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG 
ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG 
GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT 
CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG 
CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC 
GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT 
CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC 
CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG 
CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT 
GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT 
CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA 
GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC 
ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC 
GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC 
AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC 
GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG 
GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC 
ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC 
CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT 
GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC 
TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fyco1a ( XM_009297090.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295365.1 Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X1, mRNA.

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CloneID	ODa44696
Clone ID Related Accession (Same CDS sequence)	XM_009297090.2 , XM_009297091.2 , XM_009297090.3 , XM_009297091.3
Accession Version	XM_009297090.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	FYVE and coiled-coil domain-containing protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297090.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC 
AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG 
GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG 
GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG 
ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA 
GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG 
AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT 
GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT 
GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT 
AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG 
AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG 
AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA 
AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT 
GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT 
CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG 
GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC 
ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG 
GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT 
TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG 
AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA 
GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG 
GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG 
ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG 
CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG 
AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT 
CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC 
GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT 
GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG 
GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC 
GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA 
GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT 
CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG 
GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG 
AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG 
AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT 
CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG 
GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT 
CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT 
CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA 
TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT 
CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC 
TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG 
CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG 
AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT 
CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG 
AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC 
GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT 
CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA 
GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG 
GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC 
ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT 
CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA 
GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT 
GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG 
ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG 
GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT 
CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG 
CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC 
GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT 
CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC 
CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG 
CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT 
GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT 
CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA 
GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC 
ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC 
GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC 
AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC 
GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG 
GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC 
ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC 
CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT 
GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC 
TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295365.1
CDS	156..4649
Translation	MATGAAVGDSQIQRIIRDLRDAVSELTKEYKESGEPITDDSSNLHKFSYKLEYLLQFDQKEKTTFLGSRKDYWDYFSDCLAKIKGANDGIRFVKSISELKTSLGKGRAFIRYSLVHQRLADTLQQCLMNSRVTSDWYNPRSPFLKPHLSVDIISYLYELNDVQFDVASRGHDLDAEWPTFARRTLGMTSSPSHLWKAPSRSSSINSLASTYSQQHHEFPGSPDFGPGLLSDMSMQNSSILNDTSMSAIDELRLELDQSELKQRELIDRIQQLGDEGSELRGVVVELQRQLDVSLAAQGNHQELQRNLEVLIESEHALSREVEVLRDRETRREVSHKDLQDMLAAAERKNEELMTRLDGVLDEKGQRAASDFNSAQKIHELLNELKEAEKKRMDALAEGEEKRRHAEHLAEEVKVKDEALKEAEVKMAAWMEKGEQLQTRAVEQRNFMEKLQGALAVREKETSNLQRQLRDLQNSLENMEKQANVEKKRMQDDKEELEMKMNGLEGLLQSLRTQLKVKESDLLSSTKRVHFLERESEKLRSENQKLEYELENSTKKEAKKIDEYKDSCAKLIEQNTKLLQTVNKNEESKKELLENKSSLESELAGLRASEKQLRAQIDDAKVTVDEREQRLREENRNLDESLQKANMQLEESESSIRQKEQENKDLMEVQVTLKSALAAMQKEIRDINNQIGELEKNLGVARCNEANLNAQLKDKATQLEDREKLCEELQGRVEELESRQRDLEVEKTKAERAFVKQTEMIQSLEAQRNLAEKTQLEKSTCQAKETKEMALKLTLLEDQLGLSAKEVSKLQEEVVNLRAKLHSAVEEKDKTQAKLEVTEASCAELRILTEHLKKQAEEQNRLHVSELLQSSEHVDKLTSQLNQETSAHEKTTAALASAKEDLVALKAQNERMVLENAETRESLHRVNTEMAELGMTICKLTAEREEARERWAAEAVRIQELQQHGVKETERLNASLVALHQENSSLQEELQQTDKLSETMLELKQLLDKTEGERDAAREEITAVKFQMSTESMSLKHQMKSLQEEIDGLKDQLDTERKKKSELEAKLSELEGANVEYSRLIEEKDSHITYCETLLRESESETQQLQERASRSKEALSDVEKEREELKQKLDQVLMETQNQHLRMSAELEDLGQTKVNLEERLIELIRDKDALWQKSDALEFEQKLRAEEQWWLVDKEATHCLGCQGQFTWWLRRHHCRLCGRIFCYYCSNNYVMTKNSKKERCCRECYTQHGAVVERFTKAELNSSSDNQPSNTAAQLPPPPYIPTPRVTVTDPGERADDGAFDIITDEEVNQIYDSDSRTTNGSHEEEGGQTGDNSVSSDVTSEEQEDMISVQDAEITLLKSGELSASVPLDIDEIPHFGDGSRELFVKSSCYSTIPITVGDRGPIISWVFSSEPKSISFTVVYRETLDTPVEQAKVLIPLTRCNSHKETIQGQLKVRNAGVYTLIFDNSFSRFLSKKVNYHLTIEKPVIYDGSDFP

Target ORF information:

RefSeq Version	XM_009297090.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297090.3

ORF Insert Sequence:

ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC 
AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG 
GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG 
GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG 
ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA 
GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG 
AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT 
GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT 
GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT 
AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG 
AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG 
AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA 
AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT 
GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT 
CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG 
GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC 
ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG 
GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT 
TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG 
AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA 
GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG 
GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG 
ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG 
CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG 
AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT 
CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC 
GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT 
GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG 
GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC 
GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA 
GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT 
CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG 
GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG 
AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG 
AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT 
CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG 
GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT 
CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT 
CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA 
TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT 
CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC 
TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG 
CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG 
AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT 
CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG 
AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC 
GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT 
CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA 
GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG 
GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC 
ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT 
CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA 
GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT 
GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG 
ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG 
GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT 
CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG 
CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC 
GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT 
CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC 
CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG 
CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT 
GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT 
CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA 
GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC 
ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC 
GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC 
AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC 
GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG 
GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC 
ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC 
CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT 
GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC 
TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fyco1a ( XM_009297091.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295366.1 Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X2, mRNA.

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CloneID	ODa44696
Clone ID Related Accession (Same CDS sequence)	XM_009297090.2 , XM_009297091.2 , XM_009297090.3 , XM_009297091.3
Accession Version	XM_009297091.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	FYVE and coiled-coil domain-containing protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297091.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC 
AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG 
GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG 
GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG 
ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA 
GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG 
AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT 
GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT 
GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT 
AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG 
AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG 
AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA 
AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT 
GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT 
CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG 
GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC 
ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG 
GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT 
TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG 
AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA 
GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG 
GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG 
ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG 
CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG 
AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT 
CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC 
GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT 
GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG 
GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC 
GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA 
GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT 
CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG 
GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG 
AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG 
AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT 
CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG 
GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT 
CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT 
CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA 
TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT 
CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC 
TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG 
CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG 
AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT 
CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG 
AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC 
GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT 
CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA 
GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG 
GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC 
ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT 
CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA 
GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT 
GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG 
ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG 
GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT 
CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG 
CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC 
GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT 
CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC 
CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG 
CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT 
GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT 
CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA 
GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC 
ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC 
GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC 
AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC 
GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG 
GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC 
ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC 
CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT 
GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC 
TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295366.1
CDS	83..4576
Translation	MATGAAVGDSQIQRIIRDLRDAVSELTKEYKESGEPITDDSSNLHKFSYKLEYLLQFDQKEKTTFLGSRKDYWDYFSDCLAKIKGANDGIRFVKSISELKTSLGKGRAFIRYSLVHQRLADTLQQCLMNSRVTSDWYNPRSPFLKPHLSVDIISYLYELNDVQFDVASRGHDLDAEWPTFARRTLGMTSSPSHLWKAPSRSSSINSLASTYSQQHHEFPGSPDFGPGLLSDMSMQNSSILNDTSMSAIDELRLELDQSELKQRELIDRIQQLGDEGSELRGVVVELQRQLDVSLAAQGNHQELQRNLEVLIESEHALSREVEVLRDRETRREVSHKDLQDMLAAAERKNEELMTRLDGVLDEKGQRAASDFNSAQKIHELLNELKEAEKKRMDALAEGEEKRRHAEHLAEEVKVKDEALKEAEVKMAAWMEKGEQLQTRAVEQRNFMEKLQGALAVREKETSNLQRQLRDLQNSLENMEKQANVEKKRMQDDKEELEMKMNGLEGLLQSLRTQLKVKESDLLSSTKRVHFLERESEKLRSENQKLEYELENSTKKEAKKIDEYKDSCAKLIEQNTKLLQTVNKNEESKKELLENKSSLESELAGLRASEKQLRAQIDDAKVTVDEREQRLREENRNLDESLQKANMQLEESESSIRQKEQENKDLMEVQVTLKSALAAMQKEIRDINNQIGELEKNLGVARCNEANLNAQLKDKATQLEDREKLCEELQGRVEELESRQRDLEVEKTKAERAFVKQTEMIQSLEAQRNLAEKTQLEKSTCQAKETKEMALKLTLLEDQLGLSAKEVSKLQEEVVNLRAKLHSAVEEKDKTQAKLEVTEASCAELRILTEHLKKQAEEQNRLHVSELLQSSEHVDKLTSQLNQETSAHEKTTAALASAKEDLVALKAQNERMVLENAETRESLHRVNTEMAELGMTICKLTAEREEARERWAAEAVRIQELQQHGVKETERLNASLVALHQENSSLQEELQQTDKLSETMLELKQLLDKTEGERDAAREEITAVKFQMSTESMSLKHQMKSLQEEIDGLKDQLDTERKKKSELEAKLSELEGANVEYSRLIEEKDSHITYCETLLRESESETQQLQERASRSKEALSDVEKEREELKQKLDQVLMETQNQHLRMSAELEDLGQTKVNLEERLIELIRDKDALWQKSDALEFEQKLRAEEQWWLVDKEATHCLGCQGQFTWWLRRHHCRLCGRIFCYYCSNNYVMTKNSKKERCCRECYTQHGAVVERFTKAELNSSSDNQPSNTAAQLPPPPYIPTPRVTVTDPGERADDGAFDIITDEEVNQIYDSDSRTTNGSHEEEGGQTGDNSVSSDVTSEEQEDMISVQDAEITLLKSGELSASVPLDIDEIPHFGDGSRELFVKSSCYSTIPITVGDRGPIISWVFSSEPKSISFTVVYRETLDTPVEQAKVLIPLTRCNSHKETIQGQLKVRNAGVYTLIFDNSFSRFLSKKVNYHLTIEKPVIYDGSDFP

Target ORF information:

RefSeq Version	XM_009297091.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297091.3

ORF Insert Sequence:

ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC 
AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG 
GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG 
GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG 
ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA 
GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG 
AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT 
GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT 
GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT 
AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG 
AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG 
AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA 
AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT 
GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT 
CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG 
GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC 
ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG 
GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT 
TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG 
AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA 
GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG 
GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG 
ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG 
CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG 
AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT 
CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC 
GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT 
GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG 
GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC 
GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA 
GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT 
CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG 
GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG 
AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG 
AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT 
CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG 
GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT 
CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT 
CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA 
TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT 
CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC 
TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG 
CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG 
AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT 
CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG 
AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC 
GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT 
CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA 
GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG 
GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC 
ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT 
CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA 
GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT 
GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG 
ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG 
GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT 
CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG 
CTCATTGAAC TTATTAGAGA TAAGGACGCT CTGTGGCAGA AGTCTGATGC TCTAGAGTTC 
GAGCAGAAGC TAAGAGCAGA GGAGCAGTGG TGGCTGGTGG ATAAAGAGGC CACACACTGT 
CTGGGCTGCC AGGGTCAGTT CACATGGTGG CTGCGGAGGC ATCACTGCAG GTTGTGTGGC 
CGTATCTTCT GCTACTATTG CAGCAACAAT TACGTGATGA CCAAAAACTC AAAGAAGGAG 
CGCTGCTGTC GAGAGTGTTA CACACAACAC GGCGCTGTGG TGGAGAGATT CACCAAAGCT 
GAACTCAACA GCTCATCAGA CAACCAGCCA TCAAACACTG CCGCTCAGCT GCCACCGCCT 
CCATATATAC CCACACCAAG AGTTACAGTT ACAGATCCTG GTGAGAGGGC TGATGATGGA 
GCGTTTGACA TTATTACAGA CGAGGAAGTG AACCAGATCT ATGACAGCGA CTCGCGCACC 
ACCAATGGAT CACATGAGGA GGAAGGAGGA CAAACCGGAG ATAATAGCGT ATCAAGTGAC 
GTGACGTCTG AAGAACAGGA AGACATGATT TCAGTACAGG ATGCAGAAAT CACCCTCCTC 
AAGTCAGGAG AGCTCAGTGC CTCTGTGCCT CTGGATATAG ATGAGATTCC TCATTTCGGC 
GATGGCTCCC GTGAGCTGTT TGTCAAGTCC AGTTGTTACA GCACCATTCC CATCACAGTG 
GGCGACCGCG GACCCATCAT CAGCTGGGTT TTCTCCTCCG AGCCAAAGAG CATCTCCTTC 
ACTGTGGTGT ACAGAGAGAC ACTTGACACA CCTGTCGAGC AGGCCAAGGT TCTGATTCCC 
CTCACGCGCT GTAACTCACA CAAAGAGACC ATACAGGGAC AACTCAAAGT GCGCAACGCT 
GGAGTCTACA CACTCATCTT CGATAACTCA TTCTCCAGAT TTCTCTCCAA GAAGGTCAAC 
TACCACCTGA CCATTGAGAA GCCCGTCATC TACGATGGAA GTGACTTCCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fyco1a ( XM_017353733.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209222.1 Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X3, mRNA.

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CloneID	ODa57511
Clone ID Related Accession (Same CDS sequence)	XM_017353733.2 , XM_017353733.1
Accession Version	XM_017353733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	FYVE and coiled-coil domain-containing protein 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC 
AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG 
GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG 
GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG 
ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA 
GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG 
AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT 
GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT 
GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT 
AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG 
AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG 
AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA 
AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT 
GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT 
CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG 
GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC 
ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG 
GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT 
TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG 
AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA 
GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG 
GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG 
ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG 
CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG 
AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT 
CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC 
GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT 
GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG 
GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC 
GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA 
GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT 
CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG 
GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG 
AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG 
AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT 
CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG 
GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT 
CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT 
CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA 
TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT 
CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC 
TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG 
CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG 
AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT 
CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG 
AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC 
GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT 
CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA 
GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG 
GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC 
ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT 
CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA 
GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT 
GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG 
ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG 
GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT 
CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG 
CTCATTGAAC TTATTAGGTC TGTGTTTCAC CTAAAACTGC AGCTATTTTT TCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209222.1
CDS	156..3692
Translation	MATGAAVGDSQIQRIIRDLRDAVSELTKEYKESGEPITDDSSNLHKFSYKLEYLLQFDQKEKTTFLGSRKDYWDYFSDCLAKIKGANDGIRFVKSISELKTSLGKGRAFIRYSLVHQRLADTLQQCLMNSRVTSDWYNPRSPFLKPHLSVDIISYLYELNDVQFDVASRGHDLDAEWPTFARRTLGMTSSPSHLWKAPSRSSSINSLASTYSQQHHEFPGSPDFGPGLLSDMSMQNSSILNDTSMSAIDELRLELDQSELKQRELIDRIQQLGDEGSELRGVVVELQRQLDVSLAAQGNHQELQRNLEVLIESEHALSREVEVLRDRETRREVSHKDLQDMLAAAERKNEELMTRLDGVLDEKGQRAASDFNSAQKIHELLNELKEAEKKRMDALAEGEEKRRHAEHLAEEVKVKDEALKEAEVKMAAWMEKGEQLQTRAVEQRNFMEKLQGALAVREKETSNLQRQLRDLQNSLENMEKQANVEKKRMQDDKEELEMKMNGLEGLLQSLRTQLKVKESDLLSSTKRVHFLERESEKLRSENQKLEYELENSTKKEAKKIDEYKDSCAKLIEQNTKLLQTVNKNEESKKELLENKSSLESELAGLRASEKQLRAQIDDAKVTVDEREQRLREENRNLDESLQKANMQLEESESSIRQKEQENKDLMEVQVTLKSALAAMQKEIRDINNQIGELEKNLGVARCNEANLNAQLKDKATQLEDREKLCEELQGRVEELESRQRDLEVEKTKAERAFVKQTEMIQSLEAQRNLAEKTQLEKSTCQAKETKEMALKLTLLEDQLGLSAKEVSKLQEEVVNLRAKLHSAVEEKDKTQAKLEVTEASCAELRILTEHLKKQAEEQNRLHVSELLQSSEHVDKLTSQLNQETSAHEKTTAALASAKEDLVALKAQNERMVLENAETRESLHRVNTEMAELGMTICKLTAEREEARERWAAEAVRIQELQQHGVKETERLNASLVALHQENSSLQEELQQTDKLSETMLELKQLLDKTEGERDAAREEITAVKFQMSTESMSLKHQMKSLQEEIDGLKDQLDTERKKKSELEAKLSELEGANVEYSRLIEEKDSHITYCETLLRESESETQQLQERASRSKEALSDVEKEREELKQKLDQVLMETQNQHLRMSAELEDLGQTKVNLEERLIELIRSVFHLKLQLFFL

Target ORF information:

RefSeq Version	XM_017353733.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353733.2

ORF Insert Sequence:

ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC 
AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG 
GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG 
GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG 
ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA 
GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG 
AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT 
GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT 
GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT 
AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG 
AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG 
AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA 
AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT 
GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT 
CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG 
GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC 
ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG 
GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT 
TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG 
AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA 
GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG 
GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG 
ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG 
CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG 
AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT 
CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC 
GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT 
GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG 
GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC 
GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA 
GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT 
CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG 
GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG 
AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG 
AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT 
CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG 
GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT 
CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT 
CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA 
TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT 
CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC 
TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG 
CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG 
AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT 
CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG 
AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC 
GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT 
CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA 
GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG 
GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC 
ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT 
CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA 
GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT 
GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG 
ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG 
GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT 
CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG 
CTCATTGAAC TTATTAGGTC TGTGTTTCAC CTAAAACTGC AGCTATTTTT TCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fyco1a ( XM_017353733.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209222.1 Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa57511
Clone ID Related Accession (Same CDS sequence)	XM_017353733.2 , XM_017353733.1
Accession Version	XM_017353733.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	FYVE and coiled-coil domain-containing protein 1-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC 
AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG 
GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG 
GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG 
ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA 
GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG 
AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT 
GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT 
GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT 
AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG 
AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG 
AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA 
AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT 
GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT 
CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG 
GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC 
ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG 
GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT 
TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG 
AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA 
GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG 
GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG 
ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG 
CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG 
AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT 
CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC 
GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT 
GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG 
GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC 
GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA 
GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT 
CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG 
GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG 
AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG 
AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT 
CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG 
GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT 
CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT 
CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA 
TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT 
CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC 
TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG 
CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG 
AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT 
CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG 
AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC 
GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT 
CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA 
GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG 
GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC 
ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT 
CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA 
GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT 
GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG 
ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG 
GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT 
CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG 
CTCATTGAAC TTATTAGGTC TGTGTTTCAC CTAAAACTGC AGCTATTTTT TCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209222.1
CDS	134..3670
Translation	MATGAAVGDSQIQRIIRDLRDAVSELTKEYKESGEPITDDSSNLHKFSYKLEYLLQFDQKEKTTFLGSRKDYWDYFSDCLAKIKGANDGIRFVKSISELKTSLGKGRAFIRYSLVHQRLADTLQQCLMNSRVTSDWYNPRSPFLKPHLSVDIISYLYELNDVQFDVASRGHDLDAEWPTFARRTLGMTSSPSHLWKAPSRSSSINSLASTYSQQHHEFPGSPDFGPGLLSDMSMQNSSILNDTSMSAIDELRLELDQSELKQRELIDRIQQLGDEGSELRGVVVELQRQLDVSLAAQGNHQELQRNLEVLIESEHALSREVEVLRDRETRREVSHKDLQDMLAAAERKNEELMTRLDGVLDEKGQRAASDFNSAQKIHELLNELKEAEKKRMDALAEGEEKRRHAEHLAEEVKVKDEALKEAEVKMAAWMEKGEQLQTRAVEQRNFMEKLQGALAVREKETSNLQRQLRDLQNSLENMEKQANVEKKRMQDDKEELEMKMNGLEGLLQSLRTQLKVKESDLLSSTKRVHFLERESEKLRSENQKLEYELENSTKKEAKKIDEYKDSCAKLIEQNTKLLQTVNKNEESKKELLENKSSLESELAGLRASEKQLRAQIDDAKVTVDEREQRLREENRNLDESLQKANMQLEESESSIRQKEQENKDLMEVQVTLKSALAAMQKEIRDINNQIGELEKNLGVARCNEANLNAQLKDKATQLEDREKLCEELQGRVEELESRQRDLEVEKTKAERAFVKQTEMIQSLEAQRNLAEKTQLEKSTCQAKETKEMALKLTLLEDQLGLSAKEVSKLQEEVVNLRAKLHSAVEEKDKTQAKLEVTEASCAELRILTEHLKKQAEEQNRLHVSELLQSSEHVDKLTSQLNQETSAHEKTTAALASAKEDLVALKAQNERMVLENAETRESLHRVNTEMAELGMTICKLTAEREEARERWAAEAVRIQELQQHGVKETERLNASLVALHQENSSLQEELQQTDKLSETMLELKQLLDKTEGERDAAREEITAVKFQMSTESMSLKHQMKSLQEEIDGLKDQLDTERKKKSELEAKLSELEGANVEYSRLIEEKDSHITYCETLLRESESETQQLQERASRSKEALSDVEKEREELKQKLDQVLMETQNQHLRMSAELEDLGQTKVNLEERLIELIRSVFHLKLQLFFL

Target ORF information:

RefSeq Version	XM_017353733.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio FYVE and coiled-coil domain containing 1a (fyco1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353733.1

ORF Insert Sequence:

ATGGCCACTG GTGCTGCAGT CGGGGACAGT CAGATCCAGA GGATTATCAG AGATCTGCGC 
GATGCTGTTT CCGAACTGAC AAAGGAGTAT AAAGAGAGCG GTGAGCCCAT TACTGATGAC 
AGCTCCAATC TGCACAAGTT CTCCTACAAG CTGGAGTATC TGCTTCAGTT CGACCAAAAG 
GAGAAAACAA CATTTCTGGG CTCCAGAAAA GACTACTGGG ATTACTTCAG CGACTGCCTG 
GCCAAAATCA AAGGAGCAAA TGATGGAATT CGCTTCGTTA AATCAATTTC AGAGCTGAAG 
ACGTCTCTGG GCAAGGGCAG AGCATTTATC CGTTACTCTC TGGTGCATCA GCGGCTGGCA 
GACACACTCC AGCAGTGCCT CATGAACTCC AGAGTCACCA GTGACTGGTA TAATCCGAGG 
AGTCCGTTTC TCAAACCCCA TCTCAGCGTG GACATCATTA GTTATCTCTA CGAGCTCAAT 
GATGTCCAGT TTGATGTGGC ATCCAGAGGT CATGACCTTG ACGCAGAATG GCCCACTTTT 
GCCAGGAGGA CATTAGGAAT GACTAGCTCT CCCAGTCACT TGTGGAAAGC ACCGAGTCGT 
AGTTCCAGCA TCAACAGTCT GGCCAGCACA TACTCACAGC AGCACCATGA GTTCCCTGGG 
AGTCCAGACT TTGGACCTGG GCTTTTATCA GATATGTCTA TGCAAAACTC CAGCATACTG 
AATGATACCT CAATGAGTGC TATAGACGAA CTCCGTCTGG AGCTTGACCA GTCTGAATTA 
AAGCAAAGAG AGCTTATTGA CCGGATTCAG CAGTTAGGTG ATGAGGGGAG TGAACTGCGT 
GGAGTTGTTG TGGAACTGCA AAGGCAGCTA GATGTTTCTT TAGCGGCCCA AGGTAACCAT 
CAGGAGCTAC AACGTAACCT GGAGGTCTTA ATTGAGAGCG AACATGCACT CTCCCGTGAG 
GTGGAGGTCT TAAGAGATCG AGAAACCAGG AGGGAGGTTT CCCATAAAGA CCTTCAGGAC 
ATGCTGGCAG CAGCGGAGCG TAAAAACGAG GAGCTGATGA CAAGGCTAGA TGGAGTATTG 
GATGAGAAAG GTCAAAGGGC GGCCAGTGAT TTCAACTCAG CCCAGAAGAT TCACGAGCTT 
TTGAATGAAT TAAAAGAGGC TGAGAAGAAG AGGATGGATG CTCTGGCTGA AGGAGAAGAG 
AAGAGGAGGC ATGCTGAACA TCTTGCCGAG GAAGTCAAAG TGAAAGATGA AGCTCTCAAA 
GAGGCTGAGG TGAAAATGGC AGCTTGGATG GAGAAGGGTG AACAACTGCA AACCAGGGCG 
GTGGAGCAGC GTAATTTCAT GGAGAAACTT CAAGGTGCAC TTGCAGTAAG AGAGAAAGAG 
ACCAGCAACC TGCAGAGGCA ACTACGAGAT CTTCAGAACT CTCTGGAGAA CATGGAAAAG 
CAGGCCAATG TGGAGAAGAA GAGGATGCAA GATGATAAAG AAGAGCTTGA GATGAAAATG 
AATGGTCTAG AGGGGCTGTT ACAATCACTT CGGACACAGT TAAAGGTTAA AGAATCTGAT 
CTTCTCTCCA GTACCAAGAG AGTTCATTTC CTGGAGAGGG AATCAGAGAA GCTTAGGAGC 
GAGAATCAGA AGTTGGAGTA TGAGTTGGAG AACAGCACCA AAAAGGAGGC CAAGAAGATT 
GATGAATACA AGGATAGCTG CGCCAAGCTT ATTGAGCAAA ACACTAAGCT TCTGCAGACG 
GTGAATAAGA ATGAAGAGAG CAAGAAAGAG TTGCTTGAAA ACAAATCATC CTTGGAGAGC 
GAGTTGGCGG GATTGAGGGC CTCAGAGAAG CAGTTACGAG CTCAAATCGA TGATGCCAAA 
GTGACTGTGG ATGAACGAGA GCAGCGGCTG AGAGAGGAGA ACAGAAATCT GGATGAGAGT 
CTGCAGAAGG CCAACATGCA GCTGGAGGAA TCCGAGAGCT CAATTCGGCA AAAGGAGCAG 
GAGAACAAGG ACCTCATGGA GGTTCAGGTC ACTCTTAAAT CAGCTCTCGC AGCCATGCAG 
AAAGAGATAC GTGACATAAA CAACCAGATC GGTGAACTTG AGAAGAATCT TGGAGTTGCG 
AGGTGTAACG AAGCAAATCT GAACGCGCAG CTTAAAGATA AGGCCACCCA GCTGGAAGAT 
CGAGAGAAGC TCTGTGAGGA GCTCCAGGGA AGAGTGGAAG AACTGGAAAG TCGGCAGAGG 
GATTTGGAAG TGGAGAAGAC AAAAGCAGAA AGAGCCTTTG TTAAACAAAC AGAGATGATT 
CAGAGTCTTG AAGCACAGAG AAACCTGGCA GAGAAGACAC AACTTGAAAA AAGCACATGT 
CAAGCTAAAG AAACTAAAGA GATGGCCTTA AAATTGACTC TTCTAGAAGA TCAACTGGGA 
TTGAGTGCGA AGGAAGTTTC TAAGCTACAA GAGGAAGTGG TTAATCTCAG GGCTAAACTT 
CACAGTGCTG TGGAGGAGAA GGACAAGACG CAAGCAAAGC TTGAGGTCAC CGAAGCTTCC 
TGTGCTGAAC TGCGGATCTT GACTGAGCAC TTGAAGAAGC AGGCCGAGGA GCAGAATCGG 
CTGCATGTGA GCGAGCTGCT GCAGTCTAGT GAACATGTTG ATAAATTAAC TTCGCAGCTG 
AACCAGGAGA CCTCAGCGCA TGAGAAAACC ACTGCAGCAC TTGCTTCCGC TAAAGAGGAT 
CTAGTGGCTC TCAAGGCCCA GAATGAGCGG ATGGTCCTGG AGAATGCGGA AACCAGAGAG 
AGTCTCCACC GGGTCAACAC TGAAATGGCA GAGTTGGGAA TGACTATTTG CAAGCTCACC 
GCCGAGCGGG AAGAGGCCCG GGAACGTTGG GCCGCTGAAG CTGTACGCAT CCAAGAACTT 
CAGCAGCACG GAGTGAAGGA GACAGAGCGA CTCAATGCCA GCTTGGTGGC TCTGCATCAA 
GAAAACTCCA GTCTGCAGGA AGAGCTCCAG CAAACAGACA AACTGTCAGA AACCATGCTG 
GAGCTAAAGC AGCTGCTGGA CAAAACTGAG GGCGAACGGG ACGCTGCACG GGAAGAGATC 
ACCGCTGTGA AATTCCAGAT GAGCACAGAG AGCATGTCTC TCAAGCACCA AATGAAGAGT 
CTTCAAGAAG AGATTGACGG TTTGAAAGAT CAGCTAGACA CAGAGAGGAA GAAGAAATCA 
GAGCTGGAGG CCAAATTATC CGAACTAGAG GGAGCGAATG TGGAATACTC TCGTCTGATT 
GAGGAGAAGG ACTCGCACAT TACTTACTGT GAGACTTTGC TGCGTGAGAG CGAGTCTGAG 
ACACAGCAAC TGCAGGAAAG AGCATCAAGA TCCAAAGAGG CTCTTAGTGA TGTAGAGAAG 
GAGAGGGAAG AATTGAAGCA GAAATTGGAT CAAGTGTTGA TGGAGACTCA GAACCAGCAT 
CTCCGCATGT CTGCTGAACT GGAGGACCTG GGACAAACTA AAGTCAACCT GGAGGAACGG 
CTCATTGAAC TTATTAGGTC TGTGTTTCAC CTAAAACTGC AGCTATTTTT TCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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fyco1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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