slc12a3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc12a3
Entrez Gene ID	565439
Full Name	solute carrier family 12 (sodium/chloride transporter), member 3
Synonyms	si:ch211-122c9.4,wu:fj83c03
General protein information	Preferred Names solute carrier family 12 (sodium/chloride transporter), member 3 Names solute carrier family 12 member 3 NCCT solute carrier family 12 (sodium/chloride transporters), member 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045080.1	NP_001038545.1	solute carrier family 12 member 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Slc12a3	NP_062288.2
Rattus norvegicus (Norway rat)	Slc12a3	NP_062218.3
Xenopus tropicalis (tropical clawed frog)	slc12a3	XP_002937217.2
Gallus gallus (chicken)	SLC12A3	XP_414059.4
Macaca mulatta (Rhesus monkey)	SLC12A3	XP_001093467.2
Canis lupus familiaris (dog)	SLC12A3	XP_005617594.1
Bos taurus (cattle)	SLC12A3	NP_001193107.1
Danio rerio (zebrafish)	slc12a3	NP_001038545.1
Pan troglodytes (chimpanzee)	SLC12A3	XP_510983.2
Homo sapiens (human)	SLC12A3	NP_000330.2

Gene Ontology
Bibliography

Related articles in PubMed

Prostaglandin signaling regulates renal multiciliated cell specification and maturation.
Marra AN, Adeeb BD, Chambers BE, Drummond BE, Ulrich M, Addiego A, Springer M, Poureetezadi SJ, Chambers JM, Ronshaugen M, Wingert RA
Proceedings of the National Academy of Sciences of the United States of America116(17)8409-8418(2019 Apr)

Wnt signaling mediates new nephron formation during zebrafish kidney regeneration.
Kamei CN, Gallegos TF, Liu Y, Hukriede N, Drummond IA
Development (Cambridge, England)146(8)(2019 04)

Iroquois transcription factor irx2a is required for multiciliated and transporter cell fate decisions during zebrafish pronephros development.
Marra AN, Cheng CN, Adeeb B, Addiego A, Wesselman HM, Chambers BE, Chambers JM, Wingert RA
Scientific reports9(1)6454(2019 Apr)

Homeogene emx1 is required for nephron distal segment development in zebrafish.
Morales EE, Handa N, Drummond BE, Chambers JM, Marra AN, Addiego A, Wingert RA
Scientific reports8(1)18038(2018 12)

Depletion of Tissue-Specific Ion Transporters Causes Differential Expression of PRL Targets in Response to Increased Levels of Endogenous PRL.
Shu T, Shu Y, Gao Y, Jin X, He J, Zhai G, Yin Z
Frontiers in endocrinology9683(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following slc12a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03148	NM_001045080.1 Latest version!	Danio rerio solute carrier family 12 member 3 (slc12a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc12a3 ( NM_001045080.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038545.1 Danio rerio solute carrier family 12 member 3 (slc12a3), mRNA.

Price & Availability↑

CloneID	ODa03148
Clone ID Related Accession (Same CDS sequence)	NM_001045080.1
Accession Version	NM_001045080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575648000000
Organism	Danio rerio(zebrafish)
Product	solute carrier family 12 member 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX470170.5. On or before Sep 1, 2006 this sequence version replaced XM_685134.1, XM_704308.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01038218.1, GDQQ01013683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505374 [ECO:0000348] ##Evidence-Data-END##

ATGAACCTCC AAAGCGCAAG AAGCCCATTT TCAGTCTCCT CGCTGAGCAA TGGAGACGAC 
CGTAAGTTCA GTTTGGGTGG ATGTAGCAGT TATGACACTG ACAGCGTGGC CTCACGCAGC 
TCTGGTGTCT TTTCTGGATA TGACACGCTG GATGCACCAC CGAGTTACGA CTTCTACACC 
AACACTGAGG TTTTCGGGAG AGCCAAGAAA TCCAGACCAT CTCTCTTTGA GCTGCACTCC 
AACCCTCAGG ATGACCCCTC TCCACCTCCC CTGTATGAGG AGTCCAGTGT GGACAAACAG 
AGTCCAGAGG ATGAAGATGA GCCCACTGAA CCACCTCCAG AACCCGCTCG CTTTGGATGG 
GCTCAAGGAG TTATGATCCG CTGTATGCTG AATATCTGGG GTGTCATTCT GTACCTGAGG 
CTCCCCTGGA TCACAGCTCA GGCAGGGATA GGTCTGACTT GGATTATAAT CTTGGTCTCC 
TCCTCTATAA CGGGCATAAC TGGCTTGTCC ACATCGGCCA TTGCTACTAA TGGCAAAGTC 
AAAGGGGGTG GAACATATTT TCTGATCTCT CGGAGTTTGG GTCCAGAACT TGGTGGCTCG 
ATTGGGCTTA TTTTTGCTTT TGCGAATGCT GTGGCTGTAG CCATGCACAC AGTGGGATTT 
GCCGAAACGG TGCAGGTACT CATGCAGGAG ACTGAGGTTA GCATGGTTGA CAAGCTTAAC 
GATATCCGCA TCATCGGAGT GATCACTGTC ACCTGCCTGC TGGCCATCTC AATGGCTGGA 
ATGGAATGGG AATCCAAGGC CCAAGTCTTG TTCTTCTTTG TCATAATGAT ATCCTTCGCC 
AGCTACATCA TTGGAACCAT CATACCAGCC ACACCACAGA AGCAAGCCAG AGGATTTTTC 
AGCTACCGAG CTGATATTTT TGCGACAAAC TTTGTGCCCG GCTGGCGTGG ACCGGAGGGC 
AGTTTCTTTG GCATGTTTTC CATCTTCTTC CCCTCAGCTA CAGGCATCCT TGCGGGGGCT 
AATATCTCCG GAGATCTGAA GGATCCAAAT GTCGCTATAC CTCGTGGAAC AATGCTGGCC 
ATTTTTTGGA CCACTGTGTC TTATCTTATC ATCTCAGCTA CCATTGGCTC CTGTGTGGTG 
CGTGATGCTT CTGGTGATGT GAATGACACC ATATCATCAT TGACTGGGGA GTGTCTGGGA 
GTGGGCTGCA ACTATGGCTG GAACTTTACT GACTGTATGA CCAACAACAC CTGCACTTAT 
GGACTCAGCA ACTATTACCA GAGTATGAGC ATGGTTTCTG CAGTCGCTCC TCTGATCACT 
GCTGGGATTT TTGGAGCCAC CCTTTCCTCA GCCCTAGCCT GTCTGGTGTC CGCTCCTAAG 
GTTTTCCAGT GCCTTTGCAA GGACAAACTG TATCCAGGCA TTGGATTTTT CGGGAAAGGT 
TATGGGAAGA ATAACGAACC ACTGAGAAGC TACTTGCTAG CTTATATCAT TGCTATATGC 
TTTATTCTTA TTGCCGAGCT GAACACCATT GCTCCCATCA TTTCCAACTT TTTCCTCTGC 
TCCTACGCCC TCATCAACTT CAGCTGTTTC CATGCCTCAA TAACTAACTC ACCAGGCTGG 
CGTCCAACTT TTCGATTTTA CAGCAAGTGG TTGTCTTTGC TGGGAGCGGT AGTGTCTGTG 
ATCATCATGT TTCTTCTGAC CTGGTGGGCT GCTCTCATAG CCATTGGCAT AGTCATCTTC 
CTGCTGGGAT ATGTGCTGTA TAAGAAACCT GAAGTCAACT GGGGTTCATC AATGCAAGCC 
AGCTCTTATA ACATGGCACT GTCCCAGTGT GTGGGTCTTA ATCAAGTGGA AGACCATATT 
AAAAATTACA GGCCACAGTG TTTGGTCCTC AGTGGGCCTC CCTGTGCGCG GCCTTCCCTA 
GTGGATTTTA TCGGTGCCTT CACCAAGAAC CAAAGCCTGA TGATATGCGC AAATGTCCTT 
GCAAGTGGAC CCTCGCCTGG CACCGCAGAC TCCATGAGCA GTACTCATTT AAAATGGTTA 
AACAATCGGA AGATAAAGTC CTTCTACCAC ACCGTAGTGG CTGATGATCT TCGTACCGGC 
GTACAGATGT TGCTGCAGAG TACGGGTTTG GGTCGGATGA AGCCTAATGT CTTAGTGATG 
GGATATAAAA AGAACTGGCG TAAAGTTCAA CCAGGCATTA TTGAAAACTA CGTTGGAATT 
TTGCATGATG CATTTGACCT GCAATATGGT GTTTGTGTTC TTCGAATGAA GGAGGGTCTT 
GACATAACTC GAACAATCCA AGCTCAAGTA AATCTAGGAT TTGAAACATC TACTGAACAA 
GGACTTGACA CAAACTCAAC AGCTCCTACA TCACCCACAA TTGAAGCATC ATTGGATCCT 
GAGACTCTAA TGGCCCTGAC GCAGCCCAGC ACTTTATTTC AGACTAGACA AGGAAAGAAG 
ACCATTGATG TGTATTGGCT TTCCGATGAT GGCGGTTTGA CACTATTGAT CCCTTACCTC 
TTGACCCGTA AGAAGCGCTG GGGAAGATGC AAAGTGAGGG TGTTTGTTGG AGGTGAAGCT 
CAACAGATTG AAGAACAGAA AAAGGAGCTC AAAGGTCTGA TCAGCAGGTT CCGTCTGGGT 
TTCAAGGATA TTCAAGTTCT TCCAGACATC AATGGAGCTC CGCAGTCTGA ACACATTAGA 
AAGTTTGAAG ACTTCATAGC TCCGTATAGA GTAAGCTCAG TCCAAAAGGA CGGCCAAGAA 
GCCGATGAGG CAACAAAGGA ATTCTCCTGG ATGGTGTCTG ATGAGGAAAT GGAGACGTTT 
AAAGCTAAGT CTCTTCGACA GATCCGGCTG AATGAAGTTA TTCAAGATTA CTCGAGAGAT 
GCTGCACTGA TCGTTGTGAC AATGCCGGTG GGGCGAAGAG GGTCATGTCC TAGTCCTCTT 
TACATGGCTT GGCTAGAGAT TGTGTCGCGT GATCTTCGAC CCCCAGTCCT TCTTGTCCGA 
GGCAATCAGG AGAATGTGCT GACGCAATAC TGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038545.1
CDS	5..3043
Translation	MNLQSARSPFSVSSLSNGDDRKFSLGGCSSYDTDSVASRSSGVFSGYDTLDAPPSYDFYTNTEVFGRAKKSRPSLFELHSNPQDDPSPPPLYEESSVDKQSPEDEDEPTEPPPEPARFGWAQGVMIRCMLNIWGVILYLRLPWITAQAGIGLTWIIILVSSSITGITGLSTSAIATNGKVKGGGTYFLISRSLGPELGGSIGLIFAFANAVAVAMHTVGFAETVQVLMQETEVSMVDKLNDIRIIGVITVTCLLAISMAGMEWESKAQVLFFFVIMISFASYIIGTIIPATPQKQARGFFSYRADIFATNFVPGWRGPEGSFFGMFSIFFPSATGILAGANISGDLKDPNVAIPRGTMLAIFWTTVSYLIISATIGSCVVRDASGDVNDTISSLTGECLGVGCNYGWNFTDCMTNNTCTYGLSNYYQSMSMVSAVAPLITAGIFGATLSSALACLVSAPKVFQCLCKDKLYPGIGFFGKGYGKNNEPLRSYLLAYIIAICFILIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPTFRFYSKWLSLLGAVVSVIIMFLLTWWAALIAIGIVIFLLGYVLYKKPEVNWGSSMQASSYNMALSQCVGLNQVEDHIKNYRPQCLVLSGPPCARPSLVDFIGAFTKNQSLMICANVLASGPSPGTADSMSSTHLKWLNNRKIKSFYHTVVADDLRTGVQMLLQSTGLGRMKPNVLVMGYKKNWRKVQPGIIENYVGILHDAFDLQYGVCVLRMKEGLDITRTIQAQVNLGFETSTEQGLDTNSTAPTSPTIEASLDPETLMALTQPSTLFQTRQGKKTIDVYWLSDDGGLTLLIPYLLTRKKRWGRCKVRVFVGGEAQQIEEQKKELKGLISRFRLGFKDIQVLPDINGAPQSEHIRKFEDFIAPYRVSSVQKDGQEADEATKEFSWMVSDEEMETFKAKSLRQIRLNEVIQDYSRDAALIVVTMPVGRRGSCPSPLYMAWLEIVSRDLRPPVLLVRGNQENVLTQYCQ

Target ORF information:

RefSeq Version	NM_001045080.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 12 member 3 (slc12a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045080.1

ORF Insert Sequence:

ATGAACCTCC AAAGCGCAAG AAGCCCATTT TCAGTCTCCT CGCTGAGCAA TGGAGACGAC 
CGTAAGTTCA GTTTGGGTGG ATGTAGCAGT TATGACACTG ACAGCGTGGC CTCACGCAGC 
TCTGGTGTCT TTTCTGGATA TGACACGCTG GATGCACCAC CGAGTTACGA CTTCTACACC 
AACACTGAGG TTTTCGGGAG AGCCAAGAAA TCCAGACCAT CTCTCTTTGA GCTGCACTCC 
AACCCTCAGG ATGACCCCTC TCCACCTCCC CTGTATGAGG AGTCCAGTGT GGACAAACAG 
AGTCCAGAGG ATGAAGATGA GCCCACTGAA CCACCTCCAG AACCCGCTCG CTTTGGATGG 
GCTCAAGGAG TTATGATCCG CTGTATGCTG AATATCTGGG GTGTCATTCT GTACCTGAGG 
CTCCCCTGGA TCACAGCTCA GGCAGGGATA GGTCTGACTT GGATTATAAT CTTGGTCTCC 
TCCTCTATAA CGGGCATAAC TGGCTTGTCC ACATCGGCCA TTGCTACTAA TGGCAAAGTC 
AAAGGGGGTG GAACATATTT TCTGATCTCT CGGAGTTTGG GTCCAGAACT TGGTGGCTCG 
ATTGGGCTTA TTTTTGCTTT TGCGAATGCT GTGGCTGTAG CCATGCACAC AGTGGGATTT 
GCCGAAACGG TGCAGGTACT CATGCAGGAG ACTGAGGTTA GCATGGTTGA CAAGCTTAAC 
GATATCCGCA TCATCGGAGT GATCACTGTC ACCTGCCTGC TGGCCATCTC AATGGCTGGA 
ATGGAATGGG AATCCAAGGC CCAAGTCTTG TTCTTCTTTG TCATAATGAT ATCCTTCGCC 
AGCTACATCA TTGGAACCAT CATACCAGCC ACACCACAGA AGCAAGCCAG AGGATTTTTC 
AGCTACCGAG CTGATATTTT TGCGACAAAC TTTGTGCCCG GCTGGCGTGG ACCGGAGGGC 
AGTTTCTTTG GCATGTTTTC CATCTTCTTC CCCTCAGCTA CAGGCATCCT TGCGGGGGCT 
AATATCTCCG GAGATCTGAA GGATCCAAAT GTCGCTATAC CTCGTGGAAC AATGCTGGCC 
ATTTTTTGGA CCACTGTGTC TTATCTTATC ATCTCAGCTA CCATTGGCTC CTGTGTGGTG 
CGTGATGCTT CTGGTGATGT GAATGACACC ATATCATCAT TGACTGGGGA GTGTCTGGGA 
GTGGGCTGCA ACTATGGCTG GAACTTTACT GACTGTATGA CCAACAACAC CTGCACTTAT 
GGACTCAGCA ACTATTACCA GAGTATGAGC ATGGTTTCTG CAGTCGCTCC TCTGATCACT 
GCTGGGATTT TTGGAGCCAC CCTTTCCTCA GCCCTAGCCT GTCTGGTGTC CGCTCCTAAG 
GTTTTCCAGT GCCTTTGCAA GGACAAACTG TATCCAGGCA TTGGATTTTT CGGGAAAGGT 
TATGGGAAGA ATAACGAACC ACTGAGAAGC TACTTGCTAG CTTATATCAT TGCTATATGC 
TTTATTCTTA TTGCCGAGCT GAACACCATT GCTCCCATCA TTTCCAACTT TTTCCTCTGC 
TCCTACGCCC TCATCAACTT CAGCTGTTTC CATGCCTCAA TAACTAACTC ACCAGGCTGG 
CGTCCAACTT TTCGATTTTA CAGCAAGTGG TTGTCTTTGC TGGGAGCGGT AGTGTCTGTG 
ATCATCATGT TTCTTCTGAC CTGGTGGGCT GCTCTCATAG CCATTGGCAT AGTCATCTTC 
CTGCTGGGAT ATGTGCTGTA TAAGAAACCT GAAGTCAACT GGGGTTCATC AATGCAAGCC 
AGCTCTTATA ACATGGCACT GTCCCAGTGT GTGGGTCTTA ATCAAGTGGA AGACCATATT 
AAAAATTACA GGCCACAGTG TTTGGTCCTC AGTGGGCCTC CCTGTGCGCG GCCTTCCCTA 
GTGGATTTTA TCGGTGCCTT CACCAAGAAC CAAAGCCTGA TGATATGCGC AAATGTCCTT 
GCAAGTGGAC CCTCGCCTGG CACCGCAGAC TCCATGAGCA GTACTCATTT AAAATGGTTA 
AACAATCGGA AGATAAAGTC CTTCTACCAC ACCGTAGTGG CTGATGATCT TCGTACCGGC 
GTACAGATGT TGCTGCAGAG TACGGGTTTG GGTCGGATGA AGCCTAATGT CTTAGTGATG 
GGATATAAAA AGAACTGGCG TAAAGTTCAA CCAGGCATTA TTGAAAACTA CGTTGGAATT 
TTGCATGATG CATTTGACCT GCAATATGGT GTTTGTGTTC TTCGAATGAA GGAGGGTCTT 
GACATAACTC GAACAATCCA AGCTCAAGTA AATCTAGGAT TTGAAACATC TACTGAACAA 
GGACTTGACA CAAACTCAAC AGCTCCTACA TCACCCACAA TTGAAGCATC ATTGGATCCT 
GAGACTCTAA TGGCCCTGAC GCAGCCCAGC ACTTTATTTC AGACTAGACA AGGAAAGAAG 
ACCATTGATG TGTATTGGCT TTCCGATGAT GGCGGTTTGA CACTATTGAT CCCTTACCTC 
TTGACCCGTA AGAAGCGCTG GGGAAGATGC AAAGTGAGGG TGTTTGTTGG AGGTGAAGCT 
CAACAGATTG AAGAACAGAA AAAGGAGCTC AAAGGTCTGA TCAGCAGGTT CCGTCTGGGT 
TTCAAGGATA TTCAAGTTCT TCCAGACATC AATGGAGCTC CGCAGTCTGA ACACATTAGA 
AAGTTTGAAG ACTTCATAGC TCCGTATAGA GTAAGCTCAG TCCAAAAGGA CGGCCAAGAA 
GCCGATGAGG CAACAAAGGA ATTCTCCTGG ATGGTGTCTG ATGAGGAAAT GGAGACGTTT 
AAAGCTAAGT CTCTTCGACA GATCCGGCTG AATGAAGTTA TTCAAGATTA CTCGAGAGAT 
GCTGCACTGA TCGTTGTGAC AATGCCGGTG GGGCGAAGAG GGTCATGTCC TAGTCCTCTT 
TACATGGCTT GGCTAGAGAT TGTGTCGCGT GATCTTCGAC CCCCAGTCCT TCTTGTCCGA 
GGCAATCAGG AGAATGTGCT GACGCAATAC TGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prostaglandin signaling regulates renal multiciliated cell specification and maturation.
Proceedings of the National Academy of Sciences of the United States of America116(17)8409-8418(2019 Apr)
Marra AN,Adeeb BD,Chambers BE,Drummond BE,Ulrich M,Addiego A,Springer M,Poureetezadi SJ,Chambers JM,Ronshaugen M,Wingert RA

Wnt signaling mediates new nephron formation during zebrafish kidney regeneration.
Development (Cambridge, England)146(8)(2019 04)
Kamei CN,Gallegos TF,Liu Y,Hukriede N,Drummond IA

Iroquois transcription factor irx2a is required for multiciliated and transporter cell fate decisions during zebrafish pronephros development.
Scientific reports9(1)6454(2019 Apr)
Marra AN,Cheng CN,Adeeb B,Addiego A,Wesselman HM,Chambers BE,Chambers JM,Wingert RA

Homeogene emx1 is required for nephron distal segment development in zebrafish.
Scientific reports8(1)18038(2018 12)
Morales EE,Handa N,Drummond BE,Chambers JM,Marra AN,Addiego A,Wingert RA

Depletion of Tissue-Specific Ion Transporters Causes Differential Expression of PRL Targets in Response to Increased Levels of Endogenous PRL.
Frontiers in endocrinology9683(2018)
Shu T,Shu Y,Gao Y,Jin X,He J,Zhai G,Yin Z

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slc12a3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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