usp8 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp8
Entrez Gene ID	565434
Full Name	ubiquitin specific peptidase 8
Synonyms	wu:fb15f07,wu:fb92h07
General protein information	Preferred Names ubiquitin specific peptidase 8 Names ubiquitin carboxyl-terminal hydrolase 8
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009293354.2	XP_009291629.1	ubiquitin carboxyl-terminal hydrolase 8 isoform X2
XM_017351970.1	XP_017207459.1	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
XM_009293353.2	XP_009291628.1	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
XM_009293354.3	XP_009291629.1	ubiquitin carboxyl-terminal hydrolase 8 isoform X2
XM_009293353.3	XP_009291628.1	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
XM_021467717.1	XP_021323392.1	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
XM_021467716.1	XP_021323391.1	ubiquitin carboxyl-terminal hydrolase 8 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP8	NP_005145.3
Drosophila melanogaster (fruit fly)	Ubpy	NP_650948.2
Mus musculus (house mouse)	Usp8	NP_001239509.1
Rattus norvegicus (Norway rat)	Usp8	NP_001099972.1
Gallus gallus (chicken)	USP8	XP_003641901.2
Caenorhabditis elegans (roundworm)	E01B7.1	NP_507513.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002900	XP_312004.5
Pan troglodytes (chimpanzee)	USP8	XP_001168786.1
Canis lupus familiaris (dog)	USP8	XP_535474.1
Bos taurus (cattle)	USP8	NP_001069594.1
Danio rerio (zebrafish)	usp8	XP_693811.5
Xenopus tropicalis (tropical clawed frog)	usp8	XP_004912669.1
Macaca mulatta (Rhesus monkey)	USP8	XP_001114516.1

Gene Ontology

The following usp8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa22992	XM_017351970.1 Latest version!	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa22992	XM_009293353.3 Latest version!	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa22992	XM_021467716.1 Latest version!	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa22992	XM_021467717.1 Latest version!	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa22993	XM_009293354.3 Latest version!	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa22993	XM_009293354.2	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa22992	XM_009293353.2	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp8 ( XM_017351970.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207459.1 Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa22992
Clone ID Related Accession (Same CDS sequence)	XM_017351970.1 , XM_009293353.2 , XM_009293353.3 , XM_021467717.1 , XM_021467716.1
Accession Version	XM_017351970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGCAGGTG 
AAAGAGGAGC CCAATAACAG CAAGAGTGTG TGCGATAAAG GCAGCATCAG CGCTGAGGCT 
CTGTTCCGGC TGATGAAGGA CTCCAGCATC AGTGTGTTGG TGATGGACGC TCGGCCCGCG 
CTGGACTTTC AGGAGTCTCA GATCAGCGTG CCGTCGCAAA CCTGCATCAG TGTTCCAGAG 
GAGGCCATCA GCCCAGGGGT GACGGTGAAT CAGATGGAGC TGAAGCTGCC TGCAGAGTCT 
CTGAGTGAGT GGAAGCGGCG TGGGTTTGTG GATTATGTGG TTCTGCTGGA CTGGTTCAGC 
TCCGTCTCTG ACCTCAGGCT GGGCTCCACT CTCCAGAGTC TGAAGGACGC TCTCTATAAG 
TGGGACAACA GCACTATCCT GCGCAGTGAG CCGCTGGTGT TGGACGGAGG ATACGAGAGC 
TGGCTGCTGT TTTACCCCAT GTACACCAGT AACGCTAAAG TGAAACCCCC CCGACAGCAG 
ACGCCCAGCA CACTGCCACA GCTGAACTTC ACGTACCCGT CTCTGGAGGA GCCCAAACCC 
GCGAGTGTGT GTGAAGAGCC AGCATCAGCT GCACAGATGA ACGGCAAACC CCTGGAGGCA 
GAGCCGCCCG CAGAACCCGC TACAAACACA CCACCACTCG CCCATCCCAC AAACACACAC 
ACACCACCAC TTGCCCAACC TACAACCACA CACACACCAC CACTGGCTCC ATCTGCAACC 
ACACACACAC CACCACTGGC TCCATCTGCA ACCACACACA CACCACCGCT GGCTCCACCT 
GCTCACACTG AGGCTGCAGC ACCGCCCACT GCCTCGCCCA GACACACACC ACAGGTGGAC 
CGCTCCAAGA AACCGTCAGT GATTCCCCCG TCCAGCTCTA AGCTCATCAG TGATCCTGCA 
GTCAGCGAGG GCCCGGCGCC ACCAGCAGCA CACAATGGTC CCTCTGTGCC CGATCGCTCC 
GTCAAACCCA CCACAGACCC CTCCCGAGCC CTGAGCGCGG AGCAGCGGTG GGAGATCCAG 
AAAGACACGC TGGACCTGAT GGAGAAGGCC AAGAGAGAGC AGGAGCAGCG CAGGAGGGCC 
CAGGAGCAGG AGAAGCTGGA GGACAAACAG CAGGACAGGA GGAAGCTGGA GCGGCAGACG 
GCCGAGGAGG AGGACCTGAA GGAGACCAGC CCTGAAGACA AGAGCCGCAA AACACAGAAG 
GACACACCGG CGGACGCCCC GATGAAGAGC ATGTCTCTGG ACTCACCTGC ACAGTTCAAC 
ACTAGGGAGC CACTGACCCG AGCACACAGT GAGGAGATGG GCAGGACAGT CCCGGGGCTG 
CCTGATGGCT GGATGAAGTT TCTGGACACG GTGACGGGCA CCTACAGATA TTACCACTCT 
CCCACCAACC GCGTGCACCT GTACCCTCCT GAAGTCAGCA CACCGCAGAC GCCAGCCAAA 
CACACACCGC CCGCGCAGCC GGCAGCAGAC ACGGAGACGG AGAAGCAGCG GCAGCGGGAC 
CGAGAGAGAG AACAGTCCAA ACTCAAGCGC TCATACTCCT CTCCAGACAT CAGCCAGGAG 
CTCAGCGCAG AGACCAGACA GAGACCCGCT GCAGTGCCCA GCATCAACAG AGAGAACAAG 
CCTCTAGCTG CCAGCACATA TGCGAAGGTG GAGATCACGC GTCCGTCTGC AGCAAAGATC 
CGCAACCTGA ACCCAGTGTT TGGAGGCCAG GGGCCGCTGC TGACGGGCCT CCGCAACCTG 
GGGAACACCT GCTACATGAA CTCCATCCTG CAGTGCCTGT GCAACACCGT GGCCATGGCG 
GACTACTTCA ACAGGAACTA CTATCAGGAC GACATTAACC GGGCGAATAT CCTGGGTCAT 
AAGGGCGAGG TGGCGGAGGA GTTCAGCGTG GTGATGAAGG CGCTGTGGTT GGGTCAGTAT 
AAGATGATCA GCCCGCTGGA CTTTAAGGGC ACCATCTGTA AGATCAACAG CCGCTTCTCC 
AGCTACGAGC ACCAGGACTC TCAGGAGCTG CTGCTCTTCC TGATGGACGG CCTGCATGAG 
GATCTCAACA AGGCGGACAA GCGCAGGGGC TATAAGGAGG AGGAGATCGA GCATCTGGAC 
GACGTGAGCG CGGCGGATCT GGCCTGGTCC AAACACAAGC AGCTGAACGA GTCCATCATC 
GTGGCTCTGT TTCAGGGCCA GTTCAAGTCC ACGGTTCAGT GCATGAGCTG CCAGCACAAA 
TCACGCACCT TCGAGACCTT CATGTACCTG ACGCTGGAGA TGACGGCCAG CAGCAAGTGC 
TCTCTGCAGG ACTGTCTGAA ACTGTTTCAT AAAGAAGAGC GTCTGACTGA CGGTAACCGC 
TTCTACTGCC GCCACTGTAA GACCCACCGC GACGCCATCA AGAGGATGCA GATCTGGAAG 
CTGCCGCCCA TATTACTGGT GCACCTGAAG CGGTTTAAAT ATGAGGGCCG CTGGCGGGAG 
AAGCTGCAGA CGCTGGTGGA TTTCCCTCTG GATAATCTGG ACCTGTCGCA GTACGTCATC 
GGACCCAAAA ACAACCTGAA GAAGTACAAC CTGTACGCCG TGTCTAATCA CTACGGCGGA 
ATGGACGGCG GCCACTACAC TGCCTACTGC AAGAACCCCA TGAAGCAGCG CTGGTTCAAG 
TTTGACGATC ACGAGGTCTC CGAGATCTCC AGCTCCACGG TGCGCTCGCC CGCAGCTTAC 
ATATTCTTCT ACTCCTCTCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207459.1
CDS	171..3374
Translation	MPSVSSGLKDLYLSTSLGDLNKKAEIKPDKSSTRHYVQSACKIFKAAEECRLDRDEERAYVLYMKYLSVYDLIKKRPDFKQQQEFFLSMLGPTSFKKAIEEAEKLSESLKLRYEEVEVRKKLEEKERKEEKSRREERTEKKDGRASPQANTERKDSKKQVKEEPNNSKSVCDKGSISAEALFRLMKDSSISVLVMDARPALDFQESQISVPSQTCISVPEEAISPGVTVNQMELKLPAESLSEWKRRGFVDYVVLLDWFSSVSDLRLGSTLQSLKDALYKWDNSTILRSEPLVLDGGYESWLLFYPMYTSNAKVKPPRQQTPSTLPQLNFTYPSLEEPKPASVCEEPASAAQMNGKPLEAEPPAEPATNTPPLAHPTNTHTPPLAQPTTTHTPPLAPSATTHTPPLAPSATTHTPPLAPPAHTEAAAPPTASPRHTPQVDRSKKPSVIPPSSSKLISDPAVSEGPAPPAAHNGPSVPDRSVKPTTDPSRALSAEQRWEIQKDTLDLMEKAKREQEQRRRAQEQEKLEDKQQDRRKLERQTAEEEDLKETSPEDKSRKTQKDTPADAPMKSMSLDSPAQFNTREPLTRAHSEEMGRTVPGLPDGWMKFLDTVTGTYRYYHSPTNRVHLYPPEVSTPQTPAKHTPPAQPAADTETEKQRQRDREREQSKLKRSYSSPDISQELSAETRQRPAAVPSINRENKPLAASTYAKVEITRPSAAKIRNLNPVFGGQGPLLTGLRNLGNTCYMNSILQCLCNTVAMADYFNRNYYQDDINRANILGHKGEVAEEFSVVMKALWLGQYKMISPLDFKGTICKINSRFSSYEHQDSQELLLFLMDGLHEDLNKADKRRGYKEEEIEHLDDVSAADLAWSKHKQLNESIIVALFQGQFKSTVQCMSCQHKSRTFETFMYLTLEMTASSKCSLQDCLKLFHKEERLTDGNRFYCRHCKTHRDAIKRMQIWKLPPILLVHLKRFKYEGRWREKLQTLVDFPLDNLDLSQYVIGPKNNLKKYNLYAVSNHYGGMDGGHYTAYCKNPMKQRWFKFDDHEVSEISSSTVRSPAAYIFFYSSL

Target ORF information:

RefSeq Version	XM_017351970.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351970.1

ORF Insert Sequence:

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGCAGGTG 
AAAGAGGAGC CCAATAACAG CAAGAGTGTG TGCGATAAAG GCAGCATCAG CGCTGAGGCT 
CTGTTCCGGC TGATGAAGGA CTCCAGCATC AGTGTGTTGG TGATGGACGC TCGGCCCGCG 
CTGGACTTTC AGGAGTCTCA GATCAGCGTG CCGTCGCAAA CCTGCATCAG TGTTCCAGAG 
GAGGCCATCA GCCCAGGGGT GACGGTGAAT CAGATGGAGC TGAAGCTGCC TGCAGAGTCT 
CTGAGTGAGT GGAAGCGGCG TGGGTTTGTG GATTATGTGG TTCTGCTGGA CTGGTTCAGC 
TCCGTCTCTG ACCTCAGGCT GGGCTCCACT CTCCAGAGTC TGAAGGACGC TCTCTATAAG 
TGGGACAACA GCACTATCCT GCGCAGTGAG CCGCTGGTGT TGGACGGAGG ATACGAGAGC 
TGGCTGCTGT TTTACCCCAT GTACACCAGT AACGCTAAAG TGAAACCCCC CCGACAGCAG 
ACGCCCAGCA CACTGCCACA GCTGAACTTC ACGTACCCGT CTCTGGAGGA GCCCAAACCC 
GCGAGTGTGT GTGAAGAGCC AGCATCAGCT GCACAGATGA ACGGCAAACC CCTGGAGGCA 
GAGCCGCCCG CAGAACCCGC TACAAACACA CCACCACTCG CCCATCCCAC AAACACACAC 
ACACCACCAC TTGCCCAACC TACAACCACA CACACACCAC CACTGGCTCC ATCTGCAACC 
ACACACACAC CACCACTGGC TCCATCTGCA ACCACACACA CACCACCGCT GGCTCCACCT 
GCTCACACTG AGGCTGCAGC ACCGCCCACT GCCTCGCCCA GACACACACC ACAGGTGGAC 
CGCTCCAAGA AACCGTCAGT GATTCCCCCG TCCAGCTCTA AGCTCATCAG TGATCCTGCA 
GTCAGCGAGG GCCCGGCGCC ACCAGCAGCA CACAATGGTC CCTCTGTGCC CGATCGCTCC 
GTCAAACCCA CCACAGACCC CTCCCGAGCC CTGAGCGCGG AGCAGCGGTG GGAGATCCAG 
AAAGACACGC TGGACCTGAT GGAGAAGGCC AAGAGAGAGC AGGAGCAGCG CAGGAGGGCC 
CAGGAGCAGG AGAAGCTGGA GGACAAACAG CAGGACAGGA GGAAGCTGGA GCGGCAGACG 
GCCGAGGAGG AGGACCTGAA GGAGACCAGC CCTGAAGACA AGAGCCGCAA AACACAGAAG 
GACACACCGG CGGACGCCCC GATGAAGAGC ATGTCTCTGG ACTCACCTGC ACAGTTCAAC 
ACTAGGGAGC CACTGACCCG AGCACACAGT GAGGAGATGG GCAGGACAGT CCCGGGGCTG 
CCTGATGGCT GGATGAAGTT TCTGGACACG GTGACGGGCA CCTACAGATA TTACCACTCT 
CCCACCAACC GCGTGCACCT GTACCCTCCT GAAGTCAGCA CACCGCAGAC GCCAGCCAAA 
CACACACCGC CCGCGCAGCC GGCAGCAGAC ACGGAGACGG AGAAGCAGCG GCAGCGGGAC 
CGAGAGAGAG AACAGTCCAA ACTCAAGCGC TCATACTCCT CTCCAGACAT CAGCCAGGAG 
CTCAGCGCAG AGACCAGACA GAGACCCGCT GCAGTGCCCA GCATCAACAG AGAGAACAAG 
CCTCTAGCTG CCAGCACATA TGCGAAGGTG GAGATCACGC GTCCGTCTGC AGCAAAGATC 
CGCAACCTGA ACCCAGTGTT TGGAGGCCAG GGGCCGCTGC TGACGGGCCT CCGCAACCTG 
GGGAACACCT GCTACATGAA CTCCATCCTG CAGTGCCTGT GCAACACCGT GGCCATGGCG 
GACTACTTCA ACAGGAACTA CTATCAGGAC GACATTAACC GGGCGAATAT CCTGGGTCAT 
AAGGGCGAGG TGGCGGAGGA GTTCAGCGTG GTGATGAAGG CGCTGTGGTT GGGTCAGTAT 
AAGATGATCA GCCCGCTGGA CTTTAAGGGC ACCATCTGTA AGATCAACAG CCGCTTCTCC 
AGCTACGAGC ACCAGGACTC TCAGGAGCTG CTGCTCTTCC TGATGGACGG CCTGCATGAG 
GATCTCAACA AGGCGGACAA GCGCAGGGGC TATAAGGAGG AGGAGATCGA GCATCTGGAC 
GACGTGAGCG CGGCGGATCT GGCCTGGTCC AAACACAAGC AGCTGAACGA GTCCATCATC 
GTGGCTCTGT TTCAGGGCCA GTTCAAGTCC ACGGTTCAGT GCATGAGCTG CCAGCACAAA 
TCACGCACCT TCGAGACCTT CATGTACCTG ACGCTGGAGA TGACGGCCAG CAGCAAGTGC 
TCTCTGCAGG ACTGTCTGAA ACTGTTTCAT AAAGAAGAGC GTCTGACTGA CGGTAACCGC 
TTCTACTGCC GCCACTGTAA GACCCACCGC GACGCCATCA AGAGGATGCA GATCTGGAAG 
CTGCCGCCCA TATTACTGGT GCACCTGAAG CGGTTTAAAT ATGAGGGCCG CTGGCGGGAG 
AAGCTGCAGA CGCTGGTGGA TTTCCCTCTG GATAATCTGG ACCTGTCGCA GTACGTCATC 
GGACCCAAAA ACAACCTGAA GAAGTACAAC CTGTACGCCG TGTCTAATCA CTACGGCGGA 
ATGGACGGCG GCCACTACAC TGCCTACTGC AAGAACCCCA TGAAGCAGCG CTGGTTCAAG 
TTTGACGATC ACGAGGTCTC CGAGATCTCC AGCTCCACGG TGCGCTCGCC CGCAGCTTAC 
ATATTCTTCT ACTCCTCTCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp8 ( XM_009293353.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291628.1 Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22992
Clone ID Related Accession (Same CDS sequence)	XM_017351970.1 , XM_009293353.2 , XM_009293353.3 , XM_021467717.1 , XM_021467716.1
Accession Version	XM_009293353.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGCAGGTG 
AAAGAGGAGC CCAATAACAG CAAGAGTGTG TGCGATAAAG GCAGCATCAG CGCTGAGGCT 
CTGTTCCGGC TGATGAAGGA CTCCAGCATC AGTGTGTTGG TGATGGACGC TCGGCCCGCG 
CTGGACTTTC AGGAGTCTCA GATCAGCGTG CCGTCGCAAA CCTGCATCAG TGTTCCAGAG 
GAGGCCATCA GCCCAGGGGT GACGGTGAAT CAGATGGAGC TGAAGCTGCC TGCAGAGTCT 
CTGAGTGAGT GGAAGCGGCG TGGGTTTGTG GATTATGTGG TTCTGCTGGA CTGGTTCAGC 
TCCGTCTCTG ACCTCAGGCT GGGCTCCACT CTCCAGAGTC TGAAGGACGC TCTCTATAAG 
TGGGACAACA GCACTATCCT GCGCAGTGAG CCGCTGGTGT TGGACGGAGG ATACGAGAGC 
TGGCTGCTGT TTTACCCCAT GTACACCAGT AACGCTAAAG TGAAACCCCC CCGACAGCAG 
ACGCCCAGCA CACTGCCACA GCTGAACTTC ACGTACCCGT CTCTGGAGGA GCCCAAACCC 
GCGAGTGTGT GTGAAGAGCC AGCATCAGCT GCACAGATGA ACGGCAAACC CCTGGAGGCA 
GAGCCGCCCG CAGAACCCGC TACAAACACA CCACCACTCG CCCATCCCAC AAACACACAC 
ACACCACCAC TTGCCCAACC TACAACCACA CACACACCAC CACTGGCTCC ATCTGCAACC 
ACACACACAC CACCACTGGC TCCATCTGCA ACCACACACA CACCACCGCT GGCTCCACCT 
GCTCACACTG AGGCTGCAGC ACCGCCCACT GCCTCGCCCA GACACACACC ACAGGTGGAC 
CGCTCCAAGA AACCGTCAGT GATTCCCCCG TCCAGCTCTA AGCTCATCAG TGATCCTGCA 
GTCAGCGAGG GCCCGGCGCC ACCAGCAGCA CACAATGGTC CCTCTGTGCC CGATCGCTCC 
GTCAAACCCA CCACAGACCC CTCCCGAGCC CTGAGCGCGG AGCAGCGGTG GGAGATCCAG 
AAAGACACGC TGGACCTGAT GGAGAAGGCC AAGAGAGAGC AGGAGCAGCG CAGGAGGGCC 
CAGGAGCAGG AGAAGCTGGA GGACAAACAG CAGGACAGGA GGAAGCTGGA GCGGCAGACG 
GCCGAGGAGG AGGACCTGAA GGAGACCAGC CCTGAAGACA AGAGCCGCAA AACACAGAAG 
GACACACCGG CGGACGCCCC GATGAAGAGC ATGTCTCTGG ACTCACCTGC ACAGTTCAAC 
ACTAGGGAGC CACTGACCCG AGCACACAGT GAGGAGATGG GCAGGACAGT CCCGGGGCTG 
CCTGATGGCT GGATGAAGTT TCTGGACACG GTGACGGGCA CCTACAGATA TTACCACTCT 
CCCACCAACC GCGTGCACCT GTACCCTCCT GAAGTCAGCA CACCGCAGAC GCCAGCCAAA 
CACACACCGC CCGCGCAGCC GGCAGCAGAC ACGGAGACGG AGAAGCAGCG GCAGCGGGAC 
CGAGAGAGAG AACAGTCCAA ACTCAAGCGC TCATACTCCT CTCCAGACAT CAGCCAGGAG 
CTCAGCGCAG AGACCAGACA GAGACCCGCT GCAGTGCCCA GCATCAACAG AGAGAACAAG 
CCTCTAGCTG CCAGCACATA TGCGAAGGTG GAGATCACGC GTCCGTCTGC AGCAAAGATC 
CGCAACCTGA ACCCAGTGTT TGGAGGCCAG GGGCCGCTGC TGACGGGCCT CCGCAACCTG 
GGGAACACCT GCTACATGAA CTCCATCCTG CAGTGCCTGT GCAACACCGT GGCCATGGCG 
GACTACTTCA ACAGGAACTA CTATCAGGAC GACATTAACC GGGCGAATAT CCTGGGTCAT 
AAGGGCGAGG TGGCGGAGGA GTTCAGCGTG GTGATGAAGG CGCTGTGGTT GGGTCAGTAT 
AAGATGATCA GCCCGCTGGA CTTTAAGGGC ACCATCTGTA AGATCAACAG CCGCTTCTCC 
AGCTACGAGC ACCAGGACTC TCAGGAGCTG CTGCTCTTCC TGATGGACGG CCTGCATGAG 
GATCTCAACA AGGCGGACAA GCGCAGGGGC TATAAGGAGG AGGAGATCGA GCATCTGGAC 
GACGTGAGCG CGGCGGATCT GGCCTGGTCC AAACACAAGC AGCTGAACGA GTCCATCATC 
GTGGCTCTGT TTCAGGGCCA GTTCAAGTCC ACGGTTCAGT GCATGAGCTG CCAGCACAAA 
TCACGCACCT TCGAGACCTT CATGTACCTG ACGCTGGAGA TGACGGCCAG CAGCAAGTGC 
TCTCTGCAGG ACTGTCTGAA ACTGTTTCAT AAAGAAGAGC GTCTGACTGA CGGTAACCGC 
TTCTACTGCC GCCACTGTAA GACCCACCGC GACGCCATCA AGAGGATGCA GATCTGGAAG 
CTGCCGCCCA TATTACTGGT GCACCTGAAG CGGTTTAAAT ATGAGGGCCG CTGGCGGGAG 
AAGCTGCAGA CGCTGGTGGA TTTCCCTCTG GATAATCTGG ACCTGTCGCA GTACGTCATC 
GGACCCAAAA ACAACCTGAA GAAGTACAAC CTGTACGCCG TGTCTAATCA CTACGGCGGA 
ATGGACGGCG GCCACTACAC TGCCTACTGC AAGAACCCCA TGAAGCAGCG CTGGTTCAAG 
TTTGACGATC ACGAGGTCTC CGAGATCTCC AGCTCCACGG TGCGCTCGCC CGCAGCTTAC 
ATATTCTTCT ACTCCTCTCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291628.1
CDS	177..3380
Translation	MPSVSSGLKDLYLSTSLGDLNKKAEIKPDKSSTRHYVQSACKIFKAAEECRLDRDEERAYVLYMKYLSVYDLIKKRPDFKQQQEFFLSMLGPTSFKKAIEEAEKLSESLKLRYEEVEVRKKLEEKERKEEKSRREERTEKKDGRASPQANTERKDSKKQVKEEPNNSKSVCDKGSISAEALFRLMKDSSISVLVMDARPALDFQESQISVPSQTCISVPEEAISPGVTVNQMELKLPAESLSEWKRRGFVDYVVLLDWFSSVSDLRLGSTLQSLKDALYKWDNSTILRSEPLVLDGGYESWLLFYPMYTSNAKVKPPRQQTPSTLPQLNFTYPSLEEPKPASVCEEPASAAQMNGKPLEAEPPAEPATNTPPLAHPTNTHTPPLAQPTTTHTPPLAPSATTHTPPLAPSATTHTPPLAPPAHTEAAAPPTASPRHTPQVDRSKKPSVIPPSSSKLISDPAVSEGPAPPAAHNGPSVPDRSVKPTTDPSRALSAEQRWEIQKDTLDLMEKAKREQEQRRRAQEQEKLEDKQQDRRKLERQTAEEEDLKETSPEDKSRKTQKDTPADAPMKSMSLDSPAQFNTREPLTRAHSEEMGRTVPGLPDGWMKFLDTVTGTYRYYHSPTNRVHLYPPEVSTPQTPAKHTPPAQPAADTETEKQRQRDREREQSKLKRSYSSPDISQELSAETRQRPAAVPSINRENKPLAASTYAKVEITRPSAAKIRNLNPVFGGQGPLLTGLRNLGNTCYMNSILQCLCNTVAMADYFNRNYYQDDINRANILGHKGEVAEEFSVVMKALWLGQYKMISPLDFKGTICKINSRFSSYEHQDSQELLLFLMDGLHEDLNKADKRRGYKEEEIEHLDDVSAADLAWSKHKQLNESIIVALFQGQFKSTVQCMSCQHKSRTFETFMYLTLEMTASSKCSLQDCLKLFHKEERLTDGNRFYCRHCKTHRDAIKRMQIWKLPPILLVHLKRFKYEGRWREKLQTLVDFPLDNLDLSQYVIGPKNNLKKYNLYAVSNHYGGMDGGHYTAYCKNPMKQRWFKFDDHEVSEISSSTVRSPAAYIFFYSSL

Target ORF information:

RefSeq Version	XM_009293353.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293353.2

ORF Insert Sequence:

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGCAGGTG 
AAAGAGGAGC CCAATAACAG CAAGAGTGTG TGCGATAAAG GCAGCATCAG CGCTGAGGCT 
CTGTTCCGGC TGATGAAGGA CTCCAGCATC AGTGTGTTGG TGATGGACGC TCGGCCCGCG 
CTGGACTTTC AGGAGTCTCA GATCAGCGTG CCGTCGCAAA CCTGCATCAG TGTTCCAGAG 
GAGGCCATCA GCCCAGGGGT GACGGTGAAT CAGATGGAGC TGAAGCTGCC TGCAGAGTCT 
CTGAGTGAGT GGAAGCGGCG TGGGTTTGTG GATTATGTGG TTCTGCTGGA CTGGTTCAGC 
TCCGTCTCTG ACCTCAGGCT GGGCTCCACT CTCCAGAGTC TGAAGGACGC TCTCTATAAG 
TGGGACAACA GCACTATCCT GCGCAGTGAG CCGCTGGTGT TGGACGGAGG ATACGAGAGC 
TGGCTGCTGT TTTACCCCAT GTACACCAGT AACGCTAAAG TGAAACCCCC CCGACAGCAG 
ACGCCCAGCA CACTGCCACA GCTGAACTTC ACGTACCCGT CTCTGGAGGA GCCCAAACCC 
GCGAGTGTGT GTGAAGAGCC AGCATCAGCT GCACAGATGA ACGGCAAACC CCTGGAGGCA 
GAGCCGCCCG CAGAACCCGC TACAAACACA CCACCACTCG CCCATCCCAC AAACACACAC 
ACACCACCAC TTGCCCAACC TACAACCACA CACACACCAC CACTGGCTCC ATCTGCAACC 
ACACACACAC CACCACTGGC TCCATCTGCA ACCACACACA CACCACCGCT GGCTCCACCT 
GCTCACACTG AGGCTGCAGC ACCGCCCACT GCCTCGCCCA GACACACACC ACAGGTGGAC 
CGCTCCAAGA AACCGTCAGT GATTCCCCCG TCCAGCTCTA AGCTCATCAG TGATCCTGCA 
GTCAGCGAGG GCCCGGCGCC ACCAGCAGCA CACAATGGTC CCTCTGTGCC CGATCGCTCC 
GTCAAACCCA CCACAGACCC CTCCCGAGCC CTGAGCGCGG AGCAGCGGTG GGAGATCCAG 
AAAGACACGC TGGACCTGAT GGAGAAGGCC AAGAGAGAGC AGGAGCAGCG CAGGAGGGCC 
CAGGAGCAGG AGAAGCTGGA GGACAAACAG CAGGACAGGA GGAAGCTGGA GCGGCAGACG 
GCCGAGGAGG AGGACCTGAA GGAGACCAGC CCTGAAGACA AGAGCCGCAA AACACAGAAG 
GACACACCGG CGGACGCCCC GATGAAGAGC ATGTCTCTGG ACTCACCTGC ACAGTTCAAC 
ACTAGGGAGC CACTGACCCG AGCACACAGT GAGGAGATGG GCAGGACAGT CCCGGGGCTG 
CCTGATGGCT GGATGAAGTT TCTGGACACG GTGACGGGCA CCTACAGATA TTACCACTCT 
CCCACCAACC GCGTGCACCT GTACCCTCCT GAAGTCAGCA CACCGCAGAC GCCAGCCAAA 
CACACACCGC CCGCGCAGCC GGCAGCAGAC ACGGAGACGG AGAAGCAGCG GCAGCGGGAC 
CGAGAGAGAG AACAGTCCAA ACTCAAGCGC TCATACTCCT CTCCAGACAT CAGCCAGGAG 
CTCAGCGCAG AGACCAGACA GAGACCCGCT GCAGTGCCCA GCATCAACAG AGAGAACAAG 
CCTCTAGCTG CCAGCACATA TGCGAAGGTG GAGATCACGC GTCCGTCTGC AGCAAAGATC 
CGCAACCTGA ACCCAGTGTT TGGAGGCCAG GGGCCGCTGC TGACGGGCCT CCGCAACCTG 
GGGAACACCT GCTACATGAA CTCCATCCTG CAGTGCCTGT GCAACACCGT GGCCATGGCG 
GACTACTTCA ACAGGAACTA CTATCAGGAC GACATTAACC GGGCGAATAT CCTGGGTCAT 
AAGGGCGAGG TGGCGGAGGA GTTCAGCGTG GTGATGAAGG CGCTGTGGTT GGGTCAGTAT 
AAGATGATCA GCCCGCTGGA CTTTAAGGGC ACCATCTGTA AGATCAACAG CCGCTTCTCC 
AGCTACGAGC ACCAGGACTC TCAGGAGCTG CTGCTCTTCC TGATGGACGG CCTGCATGAG 
GATCTCAACA AGGCGGACAA GCGCAGGGGC TATAAGGAGG AGGAGATCGA GCATCTGGAC 
GACGTGAGCG CGGCGGATCT GGCCTGGTCC AAACACAAGC AGCTGAACGA GTCCATCATC 
GTGGCTCTGT TTCAGGGCCA GTTCAAGTCC ACGGTTCAGT GCATGAGCTG CCAGCACAAA 
TCACGCACCT TCGAGACCTT CATGTACCTG ACGCTGGAGA TGACGGCCAG CAGCAAGTGC 
TCTCTGCAGG ACTGTCTGAA ACTGTTTCAT AAAGAAGAGC GTCTGACTGA CGGTAACCGC 
TTCTACTGCC GCCACTGTAA GACCCACCGC GACGCCATCA AGAGGATGCA GATCTGGAAG 
CTGCCGCCCA TATTACTGGT GCACCTGAAG CGGTTTAAAT ATGAGGGCCG CTGGCGGGAG 
AAGCTGCAGA CGCTGGTGGA TTTCCCTCTG GATAATCTGG ACCTGTCGCA GTACGTCATC 
GGACCCAAAA ACAACCTGAA GAAGTACAAC CTGTACGCCG TGTCTAATCA CTACGGCGGA 
ATGGACGGCG GCCACTACAC TGCCTACTGC AAGAACCCCA TGAAGCAGCG CTGGTTCAAG 
TTTGACGATC ACGAGGTCTC CGAGATCTCC AGCTCCACGG TGCGCTCGCC CGCAGCTTAC 
ATATTCTTCT ACTCCTCTCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp8 ( XM_009293353.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291628.1 Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22992
Clone ID Related Accession (Same CDS sequence)	XM_017351970.1 , XM_009293353.2 , XM_009293353.3 , XM_021467717.1 , XM_021467716.1
Accession Version	XM_009293353.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGCAGGTG 
AAAGAGGAGC CCAATAACAG CAAGAGTGTG TGCGATAAAG GCAGCATCAG CGCTGAGGCT 
CTGTTCCGGC TGATGAAGGA CTCCAGCATC AGTGTGTTGG TGATGGACGC TCGGCCCGCG 
CTGGACTTTC AGGAGTCTCA GATCAGCGTG CCGTCGCAAA CCTGCATCAG TGTTCCAGAG 
GAGGCCATCA GCCCAGGGGT GACGGTGAAT CAGATGGAGC TGAAGCTGCC TGCAGAGTCT 
CTGAGTGAGT GGAAGCGGCG TGGGTTTGTG GATTATGTGG TTCTGCTGGA CTGGTTCAGC 
TCCGTCTCTG ACCTCAGGCT GGGCTCCACT CTCCAGAGTC TGAAGGACGC TCTCTATAAG 
TGGGACAACA GCACTATCCT GCGCAGTGAG CCGCTGGTGT TGGACGGAGG ATACGAGAGC 
TGGCTGCTGT TTTACCCCAT GTACACCAGT AACGCTAAAG TGAAACCCCC CCGACAGCAG 
ACGCCCAGCA CACTGCCACA GCTGAACTTC ACGTACCCGT CTCTGGAGGA GCCCAAACCC 
GCGAGTGTGT GTGAAGAGCC AGCATCAGCT GCACAGATGA ACGGCAAACC CCTGGAGGCA 
GAGCCGCCCG CAGAACCCGC TACAAACACA CCACCACTCG CCCATCCCAC AAACACACAC 
ACACCACCAC TTGCCCAACC TACAACCACA CACACACCAC CACTGGCTCC ATCTGCAACC 
ACACACACAC CACCACTGGC TCCATCTGCA ACCACACACA CACCACCGCT GGCTCCACCT 
GCTCACACTG AGGCTGCAGC ACCGCCCACT GCCTCGCCCA GACACACACC ACAGGTGGAC 
CGCTCCAAGA AACCGTCAGT GATTCCCCCG TCCAGCTCTA AGCTCATCAG TGATCCTGCA 
GTCAGCGAGG GCCCGGCGCC ACCAGCAGCA CACAATGGTC CCTCTGTGCC CGATCGCTCC 
GTCAAACCCA CCACAGACCC CTCCCGAGCC CTGAGCGCGG AGCAGCGGTG GGAGATCCAG 
AAAGACACGC TGGACCTGAT GGAGAAGGCC AAGAGAGAGC AGGAGCAGCG CAGGAGGGCC 
CAGGAGCAGG AGAAGCTGGA GGACAAACAG CAGGACAGGA GGAAGCTGGA GCGGCAGACG 
GCCGAGGAGG AGGACCTGAA GGAGACCAGC CCTGAAGACA AGAGCCGCAA AACACAGAAG 
GACACACCGG CGGACGCCCC GATGAAGAGC ATGTCTCTGG ACTCACCTGC ACAGTTCAAC 
ACTAGGGAGC CACTGACCCG AGCACACAGT GAGGAGATGG GCAGGACAGT CCCGGGGCTG 
CCTGATGGCT GGATGAAGTT TCTGGACACG GTGACGGGCA CCTACAGATA TTACCACTCT 
CCCACCAACC GCGTGCACCT GTACCCTCCT GAAGTCAGCA CACCGCAGAC GCCAGCCAAA 
CACACACCGC CCGCGCAGCC GGCAGCAGAC ACGGAGACGG AGAAGCAGCG GCAGCGGGAC 
CGAGAGAGAG AACAGTCCAA ACTCAAGCGC TCATACTCCT CTCCAGACAT CAGCCAGGAG 
CTCAGCGCAG AGACCAGACA GAGACCCGCT GCAGTGCCCA GCATCAACAG AGAGAACAAG 
CCTCTAGCTG CCAGCACATA TGCGAAGGTG GAGATCACGC GTCCGTCTGC AGCAAAGATC 
CGCAACCTGA ACCCAGTGTT TGGAGGCCAG GGGCCGCTGC TGACGGGCCT CCGCAACCTG 
GGGAACACCT GCTACATGAA CTCCATCCTG CAGTGCCTGT GCAACACCGT GGCCATGGCG 
GACTACTTCA ACAGGAACTA CTATCAGGAC GACATTAACC GGGCGAATAT CCTGGGTCAT 
AAGGGCGAGG TGGCGGAGGA GTTCAGCGTG GTGATGAAGG CGCTGTGGTT GGGTCAGTAT 
AAGATGATCA GCCCGCTGGA CTTTAAGGGC ACCATCTGTA AGATCAACAG CCGCTTCTCC 
AGCTACGAGC ACCAGGACTC TCAGGAGCTG CTGCTCTTCC TGATGGACGG CCTGCATGAG 
GATCTCAACA AGGCGGACAA GCGCAGGGGC TATAAGGAGG AGGAGATCGA GCATCTGGAC 
GACGTGAGCG CGGCGGATCT GGCCTGGTCC AAACACAAGC AGCTGAACGA GTCCATCATC 
GTGGCTCTGT TTCAGGGCCA GTTCAAGTCC ACGGTTCAGT GCATGAGCTG CCAGCACAAA 
TCACGCACCT TCGAGACCTT CATGTACCTG ACGCTGGAGA TGACGGCCAG CAGCAAGTGC 
TCTCTGCAGG ACTGTCTGAA ACTGTTTCAT AAAGAAGAGC GTCTGACTGA CGGTAACCGC 
TTCTACTGCC GCCACTGTAA GACCCACCGC GACGCCATCA AGAGGATGCA GATCTGGAAG 
CTGCCGCCCA TATTACTGGT GCACCTGAAG CGGTTTAAAT ATGAGGGCCG CTGGCGGGAG 
AAGCTGCAGA CGCTGGTGGA TTTCCCTCTG GATAATCTGG ACCTGTCGCA GTACGTCATC 
GGACCCAAAA ACAACCTGAA GAAGTACAAC CTGTACGCCG TGTCTAATCA CTACGGCGGA 
ATGGACGGCG GCCACTACAC TGCCTACTGC AAGAACCCCA TGAAGCAGCG CTGGTTCAAG 
TTTGACGATC ACGAGGTCTC CGAGATCTCC AGCTCCACGG TGCGCTCGCC CGCAGCTTAC 
ATATTCTTCT ACTCCTCTCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291628.1
CDS	188..3391
Translation	MPSVSSGLKDLYLSTSLGDLNKKAEIKPDKSSTRHYVQSACKIFKAAEECRLDRDEERAYVLYMKYLSVYDLIKKRPDFKQQQEFFLSMLGPTSFKKAIEEAEKLSESLKLRYEEVEVRKKLEEKERKEEKSRREERTEKKDGRASPQANTERKDSKKQVKEEPNNSKSVCDKGSISAEALFRLMKDSSISVLVMDARPALDFQESQISVPSQTCISVPEEAISPGVTVNQMELKLPAESLSEWKRRGFVDYVVLLDWFSSVSDLRLGSTLQSLKDALYKWDNSTILRSEPLVLDGGYESWLLFYPMYTSNAKVKPPRQQTPSTLPQLNFTYPSLEEPKPASVCEEPASAAQMNGKPLEAEPPAEPATNTPPLAHPTNTHTPPLAQPTTTHTPPLAPSATTHTPPLAPSATTHTPPLAPPAHTEAAAPPTASPRHTPQVDRSKKPSVIPPSSSKLISDPAVSEGPAPPAAHNGPSVPDRSVKPTTDPSRALSAEQRWEIQKDTLDLMEKAKREQEQRRRAQEQEKLEDKQQDRRKLERQTAEEEDLKETSPEDKSRKTQKDTPADAPMKSMSLDSPAQFNTREPLTRAHSEEMGRTVPGLPDGWMKFLDTVTGTYRYYHSPTNRVHLYPPEVSTPQTPAKHTPPAQPAADTETEKQRQRDREREQSKLKRSYSSPDISQELSAETRQRPAAVPSINRENKPLAASTYAKVEITRPSAAKIRNLNPVFGGQGPLLTGLRNLGNTCYMNSILQCLCNTVAMADYFNRNYYQDDINRANILGHKGEVAEEFSVVMKALWLGQYKMISPLDFKGTICKINSRFSSYEHQDSQELLLFLMDGLHEDLNKADKRRGYKEEEIEHLDDVSAADLAWSKHKQLNESIIVALFQGQFKSTVQCMSCQHKSRTFETFMYLTLEMTASSKCSLQDCLKLFHKEERLTDGNRFYCRHCKTHRDAIKRMQIWKLPPILLVHLKRFKYEGRWREKLQTLVDFPLDNLDLSQYVIGPKNNLKKYNLYAVSNHYGGMDGGHYTAYCKNPMKQRWFKFDDHEVSEISSSTVRSPAAYIFFYSSL

Target ORF information:

RefSeq Version	XM_009293353.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293353.3

ORF Insert Sequence:

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGCAGGTG 
AAAGAGGAGC CCAATAACAG CAAGAGTGTG TGCGATAAAG GCAGCATCAG CGCTGAGGCT 
CTGTTCCGGC TGATGAAGGA CTCCAGCATC AGTGTGTTGG TGATGGACGC TCGGCCCGCG 
CTGGACTTTC AGGAGTCTCA GATCAGCGTG CCGTCGCAAA CCTGCATCAG TGTTCCAGAG 
GAGGCCATCA GCCCAGGGGT GACGGTGAAT CAGATGGAGC TGAAGCTGCC TGCAGAGTCT 
CTGAGTGAGT GGAAGCGGCG TGGGTTTGTG GATTATGTGG TTCTGCTGGA CTGGTTCAGC 
TCCGTCTCTG ACCTCAGGCT GGGCTCCACT CTCCAGAGTC TGAAGGACGC TCTCTATAAG 
TGGGACAACA GCACTATCCT GCGCAGTGAG CCGCTGGTGT TGGACGGAGG ATACGAGAGC 
TGGCTGCTGT TTTACCCCAT GTACACCAGT AACGCTAAAG TGAAACCCCC CCGACAGCAG 
ACGCCCAGCA CACTGCCACA GCTGAACTTC ACGTACCCGT CTCTGGAGGA GCCCAAACCC 
GCGAGTGTGT GTGAAGAGCC AGCATCAGCT GCACAGATGA ACGGCAAACC CCTGGAGGCA 
GAGCCGCCCG CAGAACCCGC TACAAACACA CCACCACTCG CCCATCCCAC AAACACACAC 
ACACCACCAC TTGCCCAACC TACAACCACA CACACACCAC CACTGGCTCC ATCTGCAACC 
ACACACACAC CACCACTGGC TCCATCTGCA ACCACACACA CACCACCGCT GGCTCCACCT 
GCTCACACTG AGGCTGCAGC ACCGCCCACT GCCTCGCCCA GACACACACC ACAGGTGGAC 
CGCTCCAAGA AACCGTCAGT GATTCCCCCG TCCAGCTCTA AGCTCATCAG TGATCCTGCA 
GTCAGCGAGG GCCCGGCGCC ACCAGCAGCA CACAATGGTC CCTCTGTGCC CGATCGCTCC 
GTCAAACCCA CCACAGACCC CTCCCGAGCC CTGAGCGCGG AGCAGCGGTG GGAGATCCAG 
AAAGACACGC TGGACCTGAT GGAGAAGGCC AAGAGAGAGC AGGAGCAGCG CAGGAGGGCC 
CAGGAGCAGG AGAAGCTGGA GGACAAACAG CAGGACAGGA GGAAGCTGGA GCGGCAGACG 
GCCGAGGAGG AGGACCTGAA GGAGACCAGC CCTGAAGACA AGAGCCGCAA AACACAGAAG 
GACACACCGG CGGACGCCCC GATGAAGAGC ATGTCTCTGG ACTCACCTGC ACAGTTCAAC 
ACTAGGGAGC CACTGACCCG AGCACACAGT GAGGAGATGG GCAGGACAGT CCCGGGGCTG 
CCTGATGGCT GGATGAAGTT TCTGGACACG GTGACGGGCA CCTACAGATA TTACCACTCT 
CCCACCAACC GCGTGCACCT GTACCCTCCT GAAGTCAGCA CACCGCAGAC GCCAGCCAAA 
CACACACCGC CCGCGCAGCC GGCAGCAGAC ACGGAGACGG AGAAGCAGCG GCAGCGGGAC 
CGAGAGAGAG AACAGTCCAA ACTCAAGCGC TCATACTCCT CTCCAGACAT CAGCCAGGAG 
CTCAGCGCAG AGACCAGACA GAGACCCGCT GCAGTGCCCA GCATCAACAG AGAGAACAAG 
CCTCTAGCTG CCAGCACATA TGCGAAGGTG GAGATCACGC GTCCGTCTGC AGCAAAGATC 
CGCAACCTGA ACCCAGTGTT TGGAGGCCAG GGGCCGCTGC TGACGGGCCT CCGCAACCTG 
GGGAACACCT GCTACATGAA CTCCATCCTG CAGTGCCTGT GCAACACCGT GGCCATGGCG 
GACTACTTCA ACAGGAACTA CTATCAGGAC GACATTAACC GGGCGAATAT CCTGGGTCAT 
AAGGGCGAGG TGGCGGAGGA GTTCAGCGTG GTGATGAAGG CGCTGTGGTT GGGTCAGTAT 
AAGATGATCA GCCCGCTGGA CTTTAAGGGC ACCATCTGTA AGATCAACAG CCGCTTCTCC 
AGCTACGAGC ACCAGGACTC TCAGGAGCTG CTGCTCTTCC TGATGGACGG CCTGCATGAG 
GATCTCAACA AGGCGGACAA GCGCAGGGGC TATAAGGAGG AGGAGATCGA GCATCTGGAC 
GACGTGAGCG CGGCGGATCT GGCCTGGTCC AAACACAAGC AGCTGAACGA GTCCATCATC 
GTGGCTCTGT TTCAGGGCCA GTTCAAGTCC ACGGTTCAGT GCATGAGCTG CCAGCACAAA 
TCACGCACCT TCGAGACCTT CATGTACCTG ACGCTGGAGA TGACGGCCAG CAGCAAGTGC 
TCTCTGCAGG ACTGTCTGAA ACTGTTTCAT AAAGAAGAGC GTCTGACTGA CGGTAACCGC 
TTCTACTGCC GCCACTGTAA GACCCACCGC GACGCCATCA AGAGGATGCA GATCTGGAAG 
CTGCCGCCCA TATTACTGGT GCACCTGAAG CGGTTTAAAT ATGAGGGCCG CTGGCGGGAG 
AAGCTGCAGA CGCTGGTGGA TTTCCCTCTG GATAATCTGG ACCTGTCGCA GTACGTCATC 
GGACCCAAAA ACAACCTGAA GAAGTACAAC CTGTACGCCG TGTCTAATCA CTACGGCGGA 
ATGGACGGCG GCCACTACAC TGCCTACTGC AAGAACCCCA TGAAGCAGCG CTGGTTCAAG 
TTTGACGATC ACGAGGTCTC CGAGATCTCC AGCTCCACGG TGCGCTCGCC CGCAGCTTAC 
ATATTCTTCT ACTCCTCTCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp8 ( XM_021467717.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323392.1 Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa22992
Clone ID Related Accession (Same CDS sequence)	XM_017351970.1 , XM_009293353.2 , XM_009293353.3 , XM_021467717.1 , XM_021467716.1
Accession Version	XM_021467717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGCAGGTG 
AAAGAGGAGC CCAATAACAG CAAGAGTGTG TGCGATAAAG GCAGCATCAG CGCTGAGGCT 
CTGTTCCGGC TGATGAAGGA CTCCAGCATC AGTGTGTTGG TGATGGACGC TCGGCCCGCG 
CTGGACTTTC AGGAGTCTCA GATCAGCGTG CCGTCGCAAA CCTGCATCAG TGTTCCAGAG 
GAGGCCATCA GCCCAGGGGT GACGGTGAAT CAGATGGAGC TGAAGCTGCC TGCAGAGTCT 
CTGAGTGAGT GGAAGCGGCG TGGGTTTGTG GATTATGTGG TTCTGCTGGA CTGGTTCAGC 
TCCGTCTCTG ACCTCAGGCT GGGCTCCACT CTCCAGAGTC TGAAGGACGC TCTCTATAAG 
TGGGACAACA GCACTATCCT GCGCAGTGAG CCGCTGGTGT TGGACGGAGG ATACGAGAGC 
TGGCTGCTGT TTTACCCCAT GTACACCAGT AACGCTAAAG TGAAACCCCC CCGACAGCAG 
ACGCCCAGCA CACTGCCACA GCTGAACTTC ACGTACCCGT CTCTGGAGGA GCCCAAACCC 
GCGAGTGTGT GTGAAGAGCC AGCATCAGCT GCACAGATGA ACGGCAAACC CCTGGAGGCA 
GAGCCGCCCG CAGAACCCGC TACAAACACA CCACCACTCG CCCATCCCAC AAACACACAC 
ACACCACCAC TTGCCCAACC TACAACCACA CACACACCAC CACTGGCTCC ATCTGCAACC 
ACACACACAC CACCACTGGC TCCATCTGCA ACCACACACA CACCACCGCT GGCTCCACCT 
GCTCACACTG AGGCTGCAGC ACCGCCCACT GCCTCGCCCA GACACACACC ACAGGTGGAC 
CGCTCCAAGA AACCGTCAGT GATTCCCCCG TCCAGCTCTA AGCTCATCAG TGATCCTGCA 
GTCAGCGAGG GCCCGGCGCC ACCAGCAGCA CACAATGGTC CCTCTGTGCC CGATCGCTCC 
GTCAAACCCA CCACAGACCC CTCCCGAGCC CTGAGCGCGG AGCAGCGGTG GGAGATCCAG 
AAAGACACGC TGGACCTGAT GGAGAAGGCC AAGAGAGAGC AGGAGCAGCG CAGGAGGGCC 
CAGGAGCAGG AGAAGCTGGA GGACAAACAG CAGGACAGGA GGAAGCTGGA GCGGCAGACG 
GCCGAGGAGG AGGACCTGAA GGAGACCAGC CCTGAAGACA AGAGCCGCAA AACACAGAAG 
GACACACCGG CGGACGCCCC GATGAAGAGC ATGTCTCTGG ACTCACCTGC ACAGTTCAAC 
ACTAGGGAGC CACTGACCCG AGCACACAGT GAGGAGATGG GCAGGACAGT CCCGGGGCTG 
CCTGATGGCT GGATGAAGTT TCTGGACACG GTGACGGGCA CCTACAGATA TTACCACTCT 
CCCACCAACC GCGTGCACCT GTACCCTCCT GAAGTCAGCA CACCGCAGAC GCCAGCCAAA 
CACACACCGC CCGCGCAGCC GGCAGCAGAC ACGGAGACGG AGAAGCAGCG GCAGCGGGAC 
CGAGAGAGAG AACAGTCCAA ACTCAAGCGC TCATACTCCT CTCCAGACAT CAGCCAGGAG 
CTCAGCGCAG AGACCAGACA GAGACCCGCT GCAGTGCCCA GCATCAACAG AGAGAACAAG 
CCTCTAGCTG CCAGCACATA TGCGAAGGTG GAGATCACGC GTCCGTCTGC AGCAAAGATC 
CGCAACCTGA ACCCAGTGTT TGGAGGCCAG GGGCCGCTGC TGACGGGCCT CCGCAACCTG 
GGGAACACCT GCTACATGAA CTCCATCCTG CAGTGCCTGT GCAACACCGT GGCCATGGCG 
GACTACTTCA ACAGGAACTA CTATCAGGAC GACATTAACC GGGCGAATAT CCTGGGTCAT 
AAGGGCGAGG TGGCGGAGGA GTTCAGCGTG GTGATGAAGG CGCTGTGGTT GGGTCAGTAT 
AAGATGATCA GCCCGCTGGA CTTTAAGGGC ACCATCTGTA AGATCAACAG CCGCTTCTCC 
AGCTACGAGC ACCAGGACTC TCAGGAGCTG CTGCTCTTCC TGATGGACGG CCTGCATGAG 
GATCTCAACA AGGCGGACAA GCGCAGGGGC TATAAGGAGG AGGAGATCGA GCATCTGGAC 
GACGTGAGCG CGGCGGATCT GGCCTGGTCC AAACACAAGC AGCTGAACGA GTCCATCATC 
GTGGCTCTGT TTCAGGGCCA GTTCAAGTCC ACGGTTCAGT GCATGAGCTG CCAGCACAAA 
TCACGCACCT TCGAGACCTT CATGTACCTG ACGCTGGAGA TGACGGCCAG CAGCAAGTGC 
TCTCTGCAGG ACTGTCTGAA ACTGTTTCAT AAAGAAGAGC GTCTGACTGA CGGTAACCGC 
TTCTACTGCC GCCACTGTAA GACCCACCGC GACGCCATCA AGAGGATGCA GATCTGGAAG 
CTGCCGCCCA TATTACTGGT GCACCTGAAG CGGTTTAAAT ATGAGGGCCG CTGGCGGGAG 
AAGCTGCAGA CGCTGGTGGA TTTCCCTCTG GATAATCTGG ACCTGTCGCA GTACGTCATC 
GGACCCAAAA ACAACCTGAA GAAGTACAAC CTGTACGCCG TGTCTAATCA CTACGGCGGA 
ATGGACGGCG GCCACTACAC TGCCTACTGC AAGAACCCCA TGAAGCAGCG CTGGTTCAAG 
TTTGACGATC ACGAGGTCTC CGAGATCTCC AGCTCCACGG TGCGCTCGCC CGCAGCTTAC 
ATATTCTTCT ACTCCTCTCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323392.1
CDS	186..3389
Translation	MPSVSSGLKDLYLSTSLGDLNKKAEIKPDKSSTRHYVQSACKIFKAAEECRLDRDEERAYVLYMKYLSVYDLIKKRPDFKQQQEFFLSMLGPTSFKKAIEEAEKLSESLKLRYEEVEVRKKLEEKERKEEKSRREERTEKKDGRASPQANTERKDSKKQVKEEPNNSKSVCDKGSISAEALFRLMKDSSISVLVMDARPALDFQESQISVPSQTCISVPEEAISPGVTVNQMELKLPAESLSEWKRRGFVDYVVLLDWFSSVSDLRLGSTLQSLKDALYKWDNSTILRSEPLVLDGGYESWLLFYPMYTSNAKVKPPRQQTPSTLPQLNFTYPSLEEPKPASVCEEPASAAQMNGKPLEAEPPAEPATNTPPLAHPTNTHTPPLAQPTTTHTPPLAPSATTHTPPLAPSATTHTPPLAPPAHTEAAAPPTASPRHTPQVDRSKKPSVIPPSSSKLISDPAVSEGPAPPAAHNGPSVPDRSVKPTTDPSRALSAEQRWEIQKDTLDLMEKAKREQEQRRRAQEQEKLEDKQQDRRKLERQTAEEEDLKETSPEDKSRKTQKDTPADAPMKSMSLDSPAQFNTREPLTRAHSEEMGRTVPGLPDGWMKFLDTVTGTYRYYHSPTNRVHLYPPEVSTPQTPAKHTPPAQPAADTETEKQRQRDREREQSKLKRSYSSPDISQELSAETRQRPAAVPSINRENKPLAASTYAKVEITRPSAAKIRNLNPVFGGQGPLLTGLRNLGNTCYMNSILQCLCNTVAMADYFNRNYYQDDINRANILGHKGEVAEEFSVVMKALWLGQYKMISPLDFKGTICKINSRFSSYEHQDSQELLLFLMDGLHEDLNKADKRRGYKEEEIEHLDDVSAADLAWSKHKQLNESIIVALFQGQFKSTVQCMSCQHKSRTFETFMYLTLEMTASSKCSLQDCLKLFHKEERLTDGNRFYCRHCKTHRDAIKRMQIWKLPPILLVHLKRFKYEGRWREKLQTLVDFPLDNLDLSQYVIGPKNNLKKYNLYAVSNHYGGMDGGHYTAYCKNPMKQRWFKFDDHEVSEISSSTVRSPAAYIFFYSSL

Target ORF information:

RefSeq Version	XM_021467717.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467717.1

ORF Insert Sequence:

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGCAGGTG 
AAAGAGGAGC CCAATAACAG CAAGAGTGTG TGCGATAAAG GCAGCATCAG CGCTGAGGCT 
CTGTTCCGGC TGATGAAGGA CTCCAGCATC AGTGTGTTGG TGATGGACGC TCGGCCCGCG 
CTGGACTTTC AGGAGTCTCA GATCAGCGTG CCGTCGCAAA CCTGCATCAG TGTTCCAGAG 
GAGGCCATCA GCCCAGGGGT GACGGTGAAT CAGATGGAGC TGAAGCTGCC TGCAGAGTCT 
CTGAGTGAGT GGAAGCGGCG TGGGTTTGTG GATTATGTGG TTCTGCTGGA CTGGTTCAGC 
TCCGTCTCTG ACCTCAGGCT GGGCTCCACT CTCCAGAGTC TGAAGGACGC TCTCTATAAG 
TGGGACAACA GCACTATCCT GCGCAGTGAG CCGCTGGTGT TGGACGGAGG ATACGAGAGC 
TGGCTGCTGT TTTACCCCAT GTACACCAGT AACGCTAAAG TGAAACCCCC CCGACAGCAG 
ACGCCCAGCA CACTGCCACA GCTGAACTTC ACGTACCCGT CTCTGGAGGA GCCCAAACCC 
GCGAGTGTGT GTGAAGAGCC AGCATCAGCT GCACAGATGA ACGGCAAACC CCTGGAGGCA 
GAGCCGCCCG CAGAACCCGC TACAAACACA CCACCACTCG CCCATCCCAC AAACACACAC 
ACACCACCAC TTGCCCAACC TACAACCACA CACACACCAC CACTGGCTCC ATCTGCAACC 
ACACACACAC CACCACTGGC TCCATCTGCA ACCACACACA CACCACCGCT GGCTCCACCT 
GCTCACACTG AGGCTGCAGC ACCGCCCACT GCCTCGCCCA GACACACACC ACAGGTGGAC 
CGCTCCAAGA AACCGTCAGT GATTCCCCCG TCCAGCTCTA AGCTCATCAG TGATCCTGCA 
GTCAGCGAGG GCCCGGCGCC ACCAGCAGCA CACAATGGTC CCTCTGTGCC CGATCGCTCC 
GTCAAACCCA CCACAGACCC CTCCCGAGCC CTGAGCGCGG AGCAGCGGTG GGAGATCCAG 
AAAGACACGC TGGACCTGAT GGAGAAGGCC AAGAGAGAGC AGGAGCAGCG CAGGAGGGCC 
CAGGAGCAGG AGAAGCTGGA GGACAAACAG CAGGACAGGA GGAAGCTGGA GCGGCAGACG 
GCCGAGGAGG AGGACCTGAA GGAGACCAGC CCTGAAGACA AGAGCCGCAA AACACAGAAG 
GACACACCGG CGGACGCCCC GATGAAGAGC ATGTCTCTGG ACTCACCTGC ACAGTTCAAC 
ACTAGGGAGC CACTGACCCG AGCACACAGT GAGGAGATGG GCAGGACAGT CCCGGGGCTG 
CCTGATGGCT GGATGAAGTT TCTGGACACG GTGACGGGCA CCTACAGATA TTACCACTCT 
CCCACCAACC GCGTGCACCT GTACCCTCCT GAAGTCAGCA CACCGCAGAC GCCAGCCAAA 
CACACACCGC CCGCGCAGCC GGCAGCAGAC ACGGAGACGG AGAAGCAGCG GCAGCGGGAC 
CGAGAGAGAG AACAGTCCAA ACTCAAGCGC TCATACTCCT CTCCAGACAT CAGCCAGGAG 
CTCAGCGCAG AGACCAGACA GAGACCCGCT GCAGTGCCCA GCATCAACAG AGAGAACAAG 
CCTCTAGCTG CCAGCACATA TGCGAAGGTG GAGATCACGC GTCCGTCTGC AGCAAAGATC 
CGCAACCTGA ACCCAGTGTT TGGAGGCCAG GGGCCGCTGC TGACGGGCCT CCGCAACCTG 
GGGAACACCT GCTACATGAA CTCCATCCTG CAGTGCCTGT GCAACACCGT GGCCATGGCG 
GACTACTTCA ACAGGAACTA CTATCAGGAC GACATTAACC GGGCGAATAT CCTGGGTCAT 
AAGGGCGAGG TGGCGGAGGA GTTCAGCGTG GTGATGAAGG CGCTGTGGTT GGGTCAGTAT 
AAGATGATCA GCCCGCTGGA CTTTAAGGGC ACCATCTGTA AGATCAACAG CCGCTTCTCC 
AGCTACGAGC ACCAGGACTC TCAGGAGCTG CTGCTCTTCC TGATGGACGG CCTGCATGAG 
GATCTCAACA AGGCGGACAA GCGCAGGGGC TATAAGGAGG AGGAGATCGA GCATCTGGAC 
GACGTGAGCG CGGCGGATCT GGCCTGGTCC AAACACAAGC AGCTGAACGA GTCCATCATC 
GTGGCTCTGT TTCAGGGCCA GTTCAAGTCC ACGGTTCAGT GCATGAGCTG CCAGCACAAA 
TCACGCACCT TCGAGACCTT CATGTACCTG ACGCTGGAGA TGACGGCCAG CAGCAAGTGC 
TCTCTGCAGG ACTGTCTGAA ACTGTTTCAT AAAGAAGAGC GTCTGACTGA CGGTAACCGC 
TTCTACTGCC GCCACTGTAA GACCCACCGC GACGCCATCA AGAGGATGCA GATCTGGAAG 
CTGCCGCCCA TATTACTGGT GCACCTGAAG CGGTTTAAAT ATGAGGGCCG CTGGCGGGAG 
AAGCTGCAGA CGCTGGTGGA TTTCCCTCTG GATAATCTGG ACCTGTCGCA GTACGTCATC 
GGACCCAAAA ACAACCTGAA GAAGTACAAC CTGTACGCCG TGTCTAATCA CTACGGCGGA 
ATGGACGGCG GCCACTACAC TGCCTACTGC AAGAACCCCA TGAAGCAGCG CTGGTTCAAG 
TTTGACGATC ACGAGGTCTC CGAGATCTCC AGCTCCACGG TGCGCTCGCC CGCAGCTTAC 
ATATTCTTCT ACTCCTCTCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp8 ( XM_021467716.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323391.1 Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X2, mRNA.

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CloneID	ODa22992
Clone ID Related Accession (Same CDS sequence)	XM_017351970.1 , XM_009293353.2 , XM_009293353.3 , XM_021467717.1 , XM_021467716.1
Accession Version	XM_021467716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGCAGGTG 
AAAGAGGAGC CCAATAACAG CAAGAGTGTG TGCGATAAAG GCAGCATCAG CGCTGAGGCT 
CTGTTCCGGC TGATGAAGGA CTCCAGCATC AGTGTGTTGG TGATGGACGC TCGGCCCGCG 
CTGGACTTTC AGGAGTCTCA GATCAGCGTG CCGTCGCAAA CCTGCATCAG TGTTCCAGAG 
GAGGCCATCA GCCCAGGGGT GACGGTGAAT CAGATGGAGC TGAAGCTGCC TGCAGAGTCT 
CTGAGTGAGT GGAAGCGGCG TGGGTTTGTG GATTATGTGG TTCTGCTGGA CTGGTTCAGC 
TCCGTCTCTG ACCTCAGGCT GGGCTCCACT CTCCAGAGTC TGAAGGACGC TCTCTATAAG 
TGGGACAACA GCACTATCCT GCGCAGTGAG CCGCTGGTGT TGGACGGAGG ATACGAGAGC 
TGGCTGCTGT TTTACCCCAT GTACACCAGT AACGCTAAAG TGAAACCCCC CCGACAGCAG 
ACGCCCAGCA CACTGCCACA GCTGAACTTC ACGTACCCGT CTCTGGAGGA GCCCAAACCC 
GCGAGTGTGT GTGAAGAGCC AGCATCAGCT GCACAGATGA ACGGCAAACC CCTGGAGGCA 
GAGCCGCCCG CAGAACCCGC TACAAACACA CCACCACTCG CCCATCCCAC AAACACACAC 
ACACCACCAC TTGCCCAACC TACAACCACA CACACACCAC CACTGGCTCC ATCTGCAACC 
ACACACACAC CACCACTGGC TCCATCTGCA ACCACACACA CACCACCGCT GGCTCCACCT 
GCTCACACTG AGGCTGCAGC ACCGCCCACT GCCTCGCCCA GACACACACC ACAGGTGGAC 
CGCTCCAAGA AACCGTCAGT GATTCCCCCG TCCAGCTCTA AGCTCATCAG TGATCCTGCA 
GTCAGCGAGG GCCCGGCGCC ACCAGCAGCA CACAATGGTC CCTCTGTGCC CGATCGCTCC 
GTCAAACCCA CCACAGACCC CTCCCGAGCC CTGAGCGCGG AGCAGCGGTG GGAGATCCAG 
AAAGACACGC TGGACCTGAT GGAGAAGGCC AAGAGAGAGC AGGAGCAGCG CAGGAGGGCC 
CAGGAGCAGG AGAAGCTGGA GGACAAACAG CAGGACAGGA GGAAGCTGGA GCGGCAGACG 
GCCGAGGAGG AGGACCTGAA GGAGACCAGC CCTGAAGACA AGAGCCGCAA AACACAGAAG 
GACACACCGG CGGACGCCCC GATGAAGAGC ATGTCTCTGG ACTCACCTGC ACAGTTCAAC 
ACTAGGGAGC CACTGACCCG AGCACACAGT GAGGAGATGG GCAGGACAGT CCCGGGGCTG 
CCTGATGGCT GGATGAAGTT TCTGGACACG GTGACGGGCA CCTACAGATA TTACCACTCT 
CCCACCAACC GCGTGCACCT GTACCCTCCT GAAGTCAGCA CACCGCAGAC GCCAGCCAAA 
CACACACCGC CCGCGCAGCC GGCAGCAGAC ACGGAGACGG AGAAGCAGCG GCAGCGGGAC 
CGAGAGAGAG AACAGTCCAA ACTCAAGCGC TCATACTCCT CTCCAGACAT CAGCCAGGAG 
CTCAGCGCAG AGACCAGACA GAGACCCGCT GCAGTGCCCA GCATCAACAG AGAGAACAAG 
CCTCTAGCTG CCAGCACATA TGCGAAGGTG GAGATCACGC GTCCGTCTGC AGCAAAGATC 
CGCAACCTGA ACCCAGTGTT TGGAGGCCAG GGGCCGCTGC TGACGGGCCT CCGCAACCTG 
GGGAACACCT GCTACATGAA CTCCATCCTG CAGTGCCTGT GCAACACCGT GGCCATGGCG 
GACTACTTCA ACAGGAACTA CTATCAGGAC GACATTAACC GGGCGAATAT CCTGGGTCAT 
AAGGGCGAGG TGGCGGAGGA GTTCAGCGTG GTGATGAAGG CGCTGTGGTT GGGTCAGTAT 
AAGATGATCA GCCCGCTGGA CTTTAAGGGC ACCATCTGTA AGATCAACAG CCGCTTCTCC 
AGCTACGAGC ACCAGGACTC TCAGGAGCTG CTGCTCTTCC TGATGGACGG CCTGCATGAG 
GATCTCAACA AGGCGGACAA GCGCAGGGGC TATAAGGAGG AGGAGATCGA GCATCTGGAC 
GACGTGAGCG CGGCGGATCT GGCCTGGTCC AAACACAAGC AGCTGAACGA GTCCATCATC 
GTGGCTCTGT TTCAGGGCCA GTTCAAGTCC ACGGTTCAGT GCATGAGCTG CCAGCACAAA 
TCACGCACCT TCGAGACCTT CATGTACCTG ACGCTGGAGA TGACGGCCAG CAGCAAGTGC 
TCTCTGCAGG ACTGTCTGAA ACTGTTTCAT AAAGAAGAGC GTCTGACTGA CGGTAACCGC 
TTCTACTGCC GCCACTGTAA GACCCACCGC GACGCCATCA AGAGGATGCA GATCTGGAAG 
CTGCCGCCCA TATTACTGGT GCACCTGAAG CGGTTTAAAT ATGAGGGCCG CTGGCGGGAG 
AAGCTGCAGA CGCTGGTGGA TTTCCCTCTG GATAATCTGG ACCTGTCGCA GTACGTCATC 
GGACCCAAAA ACAACCTGAA GAAGTACAAC CTGTACGCCG TGTCTAATCA CTACGGCGGA 
ATGGACGGCG GCCACTACAC TGCCTACTGC AAGAACCCCA TGAAGCAGCG CTGGTTCAAG 
TTTGACGATC ACGAGGTCTC CGAGATCTCC AGCTCCACGG TGCGCTCGCC CGCAGCTTAC 
ATATTCTTCT ACTCCTCTCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323391.1
CDS	176..3379
Translation	MPSVSSGLKDLYLSTSLGDLNKKAEIKPDKSSTRHYVQSACKIFKAAEECRLDRDEERAYVLYMKYLSVYDLIKKRPDFKQQQEFFLSMLGPTSFKKAIEEAEKLSESLKLRYEEVEVRKKLEEKERKEEKSRREERTEKKDGRASPQANTERKDSKKQVKEEPNNSKSVCDKGSISAEALFRLMKDSSISVLVMDARPALDFQESQISVPSQTCISVPEEAISPGVTVNQMELKLPAESLSEWKRRGFVDYVVLLDWFSSVSDLRLGSTLQSLKDALYKWDNSTILRSEPLVLDGGYESWLLFYPMYTSNAKVKPPRQQTPSTLPQLNFTYPSLEEPKPASVCEEPASAAQMNGKPLEAEPPAEPATNTPPLAHPTNTHTPPLAQPTTTHTPPLAPSATTHTPPLAPSATTHTPPLAPPAHTEAAAPPTASPRHTPQVDRSKKPSVIPPSSSKLISDPAVSEGPAPPAAHNGPSVPDRSVKPTTDPSRALSAEQRWEIQKDTLDLMEKAKREQEQRRRAQEQEKLEDKQQDRRKLERQTAEEEDLKETSPEDKSRKTQKDTPADAPMKSMSLDSPAQFNTREPLTRAHSEEMGRTVPGLPDGWMKFLDTVTGTYRYYHSPTNRVHLYPPEVSTPQTPAKHTPPAQPAADTETEKQRQRDREREQSKLKRSYSSPDISQELSAETRQRPAAVPSINRENKPLAASTYAKVEITRPSAAKIRNLNPVFGGQGPLLTGLRNLGNTCYMNSILQCLCNTVAMADYFNRNYYQDDINRANILGHKGEVAEEFSVVMKALWLGQYKMISPLDFKGTICKINSRFSSYEHQDSQELLLFLMDGLHEDLNKADKRRGYKEEEIEHLDDVSAADLAWSKHKQLNESIIVALFQGQFKSTVQCMSCQHKSRTFETFMYLTLEMTASSKCSLQDCLKLFHKEERLTDGNRFYCRHCKTHRDAIKRMQIWKLPPILLVHLKRFKYEGRWREKLQTLVDFPLDNLDLSQYVIGPKNNLKKYNLYAVSNHYGGMDGGHYTAYCKNPMKQRWFKFDDHEVSEISSSTVRSPAAYIFFYSSL

Target ORF information:

RefSeq Version	XM_021467716.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467716.1

ORF Insert Sequence:

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGCAGGTG 
AAAGAGGAGC CCAATAACAG CAAGAGTGTG TGCGATAAAG GCAGCATCAG CGCTGAGGCT 
CTGTTCCGGC TGATGAAGGA CTCCAGCATC AGTGTGTTGG TGATGGACGC TCGGCCCGCG 
CTGGACTTTC AGGAGTCTCA GATCAGCGTG CCGTCGCAAA CCTGCATCAG TGTTCCAGAG 
GAGGCCATCA GCCCAGGGGT GACGGTGAAT CAGATGGAGC TGAAGCTGCC TGCAGAGTCT 
CTGAGTGAGT GGAAGCGGCG TGGGTTTGTG GATTATGTGG TTCTGCTGGA CTGGTTCAGC 
TCCGTCTCTG ACCTCAGGCT GGGCTCCACT CTCCAGAGTC TGAAGGACGC TCTCTATAAG 
TGGGACAACA GCACTATCCT GCGCAGTGAG CCGCTGGTGT TGGACGGAGG ATACGAGAGC 
TGGCTGCTGT TTTACCCCAT GTACACCAGT AACGCTAAAG TGAAACCCCC CCGACAGCAG 
ACGCCCAGCA CACTGCCACA GCTGAACTTC ACGTACCCGT CTCTGGAGGA GCCCAAACCC 
GCGAGTGTGT GTGAAGAGCC AGCATCAGCT GCACAGATGA ACGGCAAACC CCTGGAGGCA 
GAGCCGCCCG CAGAACCCGC TACAAACACA CCACCACTCG CCCATCCCAC AAACACACAC 
ACACCACCAC TTGCCCAACC TACAACCACA CACACACCAC CACTGGCTCC ATCTGCAACC 
ACACACACAC CACCACTGGC TCCATCTGCA ACCACACACA CACCACCGCT GGCTCCACCT 
GCTCACACTG AGGCTGCAGC ACCGCCCACT GCCTCGCCCA GACACACACC ACAGGTGGAC 
CGCTCCAAGA AACCGTCAGT GATTCCCCCG TCCAGCTCTA AGCTCATCAG TGATCCTGCA 
GTCAGCGAGG GCCCGGCGCC ACCAGCAGCA CACAATGGTC CCTCTGTGCC CGATCGCTCC 
GTCAAACCCA CCACAGACCC CTCCCGAGCC CTGAGCGCGG AGCAGCGGTG GGAGATCCAG 
AAAGACACGC TGGACCTGAT GGAGAAGGCC AAGAGAGAGC AGGAGCAGCG CAGGAGGGCC 
CAGGAGCAGG AGAAGCTGGA GGACAAACAG CAGGACAGGA GGAAGCTGGA GCGGCAGACG 
GCCGAGGAGG AGGACCTGAA GGAGACCAGC CCTGAAGACA AGAGCCGCAA AACACAGAAG 
GACACACCGG CGGACGCCCC GATGAAGAGC ATGTCTCTGG ACTCACCTGC ACAGTTCAAC 
ACTAGGGAGC CACTGACCCG AGCACACAGT GAGGAGATGG GCAGGACAGT CCCGGGGCTG 
CCTGATGGCT GGATGAAGTT TCTGGACACG GTGACGGGCA CCTACAGATA TTACCACTCT 
CCCACCAACC GCGTGCACCT GTACCCTCCT GAAGTCAGCA CACCGCAGAC GCCAGCCAAA 
CACACACCGC CCGCGCAGCC GGCAGCAGAC ACGGAGACGG AGAAGCAGCG GCAGCGGGAC 
CGAGAGAGAG AACAGTCCAA ACTCAAGCGC TCATACTCCT CTCCAGACAT CAGCCAGGAG 
CTCAGCGCAG AGACCAGACA GAGACCCGCT GCAGTGCCCA GCATCAACAG AGAGAACAAG 
CCTCTAGCTG CCAGCACATA TGCGAAGGTG GAGATCACGC GTCCGTCTGC AGCAAAGATC 
CGCAACCTGA ACCCAGTGTT TGGAGGCCAG GGGCCGCTGC TGACGGGCCT CCGCAACCTG 
GGGAACACCT GCTACATGAA CTCCATCCTG CAGTGCCTGT GCAACACCGT GGCCATGGCG 
GACTACTTCA ACAGGAACTA CTATCAGGAC GACATTAACC GGGCGAATAT CCTGGGTCAT 
AAGGGCGAGG TGGCGGAGGA GTTCAGCGTG GTGATGAAGG CGCTGTGGTT GGGTCAGTAT 
AAGATGATCA GCCCGCTGGA CTTTAAGGGC ACCATCTGTA AGATCAACAG CCGCTTCTCC 
AGCTACGAGC ACCAGGACTC TCAGGAGCTG CTGCTCTTCC TGATGGACGG CCTGCATGAG 
GATCTCAACA AGGCGGACAA GCGCAGGGGC TATAAGGAGG AGGAGATCGA GCATCTGGAC 
GACGTGAGCG CGGCGGATCT GGCCTGGTCC AAACACAAGC AGCTGAACGA GTCCATCATC 
GTGGCTCTGT TTCAGGGCCA GTTCAAGTCC ACGGTTCAGT GCATGAGCTG CCAGCACAAA 
TCACGCACCT TCGAGACCTT CATGTACCTG ACGCTGGAGA TGACGGCCAG CAGCAAGTGC 
TCTCTGCAGG ACTGTCTGAA ACTGTTTCAT AAAGAAGAGC GTCTGACTGA CGGTAACCGC 
TTCTACTGCC GCCACTGTAA GACCCACCGC GACGCCATCA AGAGGATGCA GATCTGGAAG 
CTGCCGCCCA TATTACTGGT GCACCTGAAG CGGTTTAAAT ATGAGGGCCG CTGGCGGGAG 
AAGCTGCAGA CGCTGGTGGA TTTCCCTCTG GATAATCTGG ACCTGTCGCA GTACGTCATC 
GGACCCAAAA ACAACCTGAA GAAGTACAAC CTGTACGCCG TGTCTAATCA CTACGGCGGA 
ATGGACGGCG GCCACTACAC TGCCTACTGC AAGAACCCCA TGAAGCAGCG CTGGTTCAAG 
TTTGACGATC ACGAGGTCTC CGAGATCTCC AGCTCCACGG TGCGCTCGCC CGCAGCTTAC 
ATATTCTTCT ACTCCTCTCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp8 ( XM_009293354.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291629.1 Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa22993
Clone ID Related Accession (Same CDS sequence)	XM_009293354.2 , XM_009293354.3
Accession Version	XM_009293354.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 8 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGGTGAAA 
GAGGAGCCCA ATAACAGCAA GAGTGTGTGC GATAAAGGCA GCATCAGCGC TGAGGCTCTG 
TTCCGGCTGA TGAAGGACTC CAGCATCAGT GTGTTGGTGA TGGACGCTCG GCCCGCGCTG 
GACTTTCAGG AGTCTCAGAT CAGCGTGCCG TCGCAAACCT GCATCAGTGT TCCAGAGGAG 
GCCATCAGCC CAGGGGTGAC GGTGAATCAG ATGGAGCTGA AGCTGCCTGC AGAGTCTCTG 
AGTGAGTGGA AGCGGCGTGG GTTTGTGGAT TATGTGGTTC TGCTGGACTG GTTCAGCTCC 
GTCTCTGACC TCAGGCTGGG CTCCACTCTC CAGAGTCTGA AGGACGCTCT CTATAAGTGG 
GACAACAGCA CTATCCTGCG CAGTGAGCCG CTGGTGTTGG ACGGAGGATA CGAGAGCTGG 
CTGCTGTTTT ACCCCATGTA CACCAGTAAC GCTAAAGTGA AACCCCCCCG ACAGCAGACG 
CCCAGCACAC TGCCACAGCT GAACTTCACG TACCCGTCTC TGGAGGAGCC CAAACCCGCG 
AGTGTGTGTG AAGAGCCAGC ATCAGCTGCA CAGATGAACG GCAAACCCCT GGAGGCAGAG 
CCGCCCGCAG AACCCGCTAC AAACACACCA CCACTCGCCC ATCCCACAAA CACACACACA 
CCACCACTTG CCCAACCTAC AACCACACAC ACACCACCAC TGGCTCCATC TGCAACCACA 
CACACACCAC CACTGGCTCC ATCTGCAACC ACACACACAC CACCGCTGGC TCCACCTGCT 
CACACTGAGG CTGCAGCACC GCCCACTGCC TCGCCCAGAC ACACACCACA GGTGGACCGC 
TCCAAGAAAC CGTCAGTGAT TCCCCCGTCC AGCTCTAAGC TCATCAGTGA TCCTGCAGTC 
AGCGAGGGCC CGGCGCCACC AGCAGCACAC AATGGTCCCT CTGTGCCCGA TCGCTCCGTC 
AAACCCACCA CAGACCCCTC CCGAGCCCTG AGCGCGGAGC AGCGGTGGGA GATCCAGAAA 
GACACGCTGG ACCTGATGGA GAAGGCCAAG AGAGAGCAGG AGCAGCGCAG GAGGGCCCAG 
GAGCAGGAGA AGCTGGAGGA CAAACAGCAG GACAGGAGGA AGCTGGAGCG GCAGACGGCC 
GAGGAGGAGG ACCTGAAGGA GACCAGCCCT GAAGACAAGA GCCGCAAAAC ACAGAAGGAC 
ACACCGGCGG ACGCCCCGAT GAAGAGCATG TCTCTGGACT CACCTGCACA GTTCAACACT 
AGGGAGCCAC TGACCCGAGC ACACAGTGAG GAGATGGGCA GGACAGTCCC GGGGCTGCCT 
GATGGCTGGA TGAAGTTTCT GGACACGGTG ACGGGCACCT ACAGATATTA CCACTCTCCC 
ACCAACCGCG TGCACCTGTA CCCTCCTGAA GTCAGCACAC CGCAGACGCC AGCCAAACAC 
ACACCGCCCG CGCAGCCGGC AGCAGACACG GAGACGGAGA AGCAGCGGCA GCGGGACCGA 
GAGAGAGAAC AGTCCAAACT CAAGCGCTCA TACTCCTCTC CAGACATCAG CCAGGAGCTC 
AGCGCAGAGA CCAGACAGAG ACCCGCTGCA GTGCCCAGCA TCAACAGAGA GAACAAGCCT 
CTAGCTGCCA GCACATATGC GAAGGTGGAG ATCACGCGTC CGTCTGCAGC AAAGATCCGC 
AACCTGAACC CAGTGTTTGG AGGCCAGGGG CCGCTGCTGA CGGGCCTCCG CAACCTGGGG 
AACACCTGCT ACATGAACTC CATCCTGCAG TGCCTGTGCA ACACCGTGGC CATGGCGGAC 
TACTTCAACA GGAACTACTA TCAGGACGAC ATTAACCGGG CGAATATCCT GGGTCATAAG 
GGCGAGGTGG CGGAGGAGTT CAGCGTGGTG ATGAAGGCGC TGTGGTTGGG TCAGTATAAG 
ATGATCAGCC CGCTGGACTT TAAGGGCACC ATCTGTAAGA TCAACAGCCG CTTCTCCAGC 
TACGAGCACC AGGACTCTCA GGAGCTGCTG CTCTTCCTGA TGGACGGCCT GCATGAGGAT 
CTCAACAAGG CGGACAAGCG CAGGGGCTAT AAGGAGGAGG AGATCGAGCA TCTGGACGAC 
GTGAGCGCGG CGGATCTGGC CTGGTCCAAA CACAAGCAGC TGAACGAGTC CATCATCGTG 
GCTCTGTTTC AGGGCCAGTT CAAGTCCACG GTTCAGTGCA TGAGCTGCCA GCACAAATCA 
CGCACCTTCG AGACCTTCAT GTACCTGACG CTGGAGATGA CGGCCAGCAG CAAGTGCTCT 
CTGCAGGACT GTCTGAAACT GTTTCATAAA GAAGAGCGTC TGACTGACGG TAACCGCTTC 
TACTGCCGCC ACTGTAAGAC CCACCGCGAC GCCATCAAGA GGATGCAGAT CTGGAAGCTG 
CCGCCCATAT TACTGGTGCA CCTGAAGCGG TTTAAATATG AGGGCCGCTG GCGGGAGAAG 
CTGCAGACGC TGGTGGATTT CCCTCTGGAT AATCTGGACC TGTCGCAGTA CGTCATCGGA 
CCCAAAAACA ACCTGAAGAA GTACAACCTG TACGCCGTGT CTAATCACTA CGGCGGAATG 
GACGGCGGCC ACTACACTGC CTACTGCAAG AACCCCATGA AGCAGCGCTG GTTCAAGTTT 
GACGATCACG AGGTCTCCGA GATCTCCAGC TCCACGGTGC GCTCGCCCGC AGCTTACATA 
TTCTTCTACT CCTCTCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291629.1
CDS	177..3377
Translation	MPSVSSGLKDLYLSTSLGDLNKKAEIKPDKSSTRHYVQSACKIFKAAEECRLDRDEERAYVLYMKYLSVYDLIKKRPDFKQQQEFFLSMLGPTSFKKAIEEAEKLSESLKLRYEEVEVRKKLEEKERKEEKSRREERTEKKDGRASPQANTERKDSKKVKEEPNNSKSVCDKGSISAEALFRLMKDSSISVLVMDARPALDFQESQISVPSQTCISVPEEAISPGVTVNQMELKLPAESLSEWKRRGFVDYVVLLDWFSSVSDLRLGSTLQSLKDALYKWDNSTILRSEPLVLDGGYESWLLFYPMYTSNAKVKPPRQQTPSTLPQLNFTYPSLEEPKPASVCEEPASAAQMNGKPLEAEPPAEPATNTPPLAHPTNTHTPPLAQPTTTHTPPLAPSATTHTPPLAPSATTHTPPLAPPAHTEAAAPPTASPRHTPQVDRSKKPSVIPPSSSKLISDPAVSEGPAPPAAHNGPSVPDRSVKPTTDPSRALSAEQRWEIQKDTLDLMEKAKREQEQRRRAQEQEKLEDKQQDRRKLERQTAEEEDLKETSPEDKSRKTQKDTPADAPMKSMSLDSPAQFNTREPLTRAHSEEMGRTVPGLPDGWMKFLDTVTGTYRYYHSPTNRVHLYPPEVSTPQTPAKHTPPAQPAADTETEKQRQRDREREQSKLKRSYSSPDISQELSAETRQRPAAVPSINRENKPLAASTYAKVEITRPSAAKIRNLNPVFGGQGPLLTGLRNLGNTCYMNSILQCLCNTVAMADYFNRNYYQDDINRANILGHKGEVAEEFSVVMKALWLGQYKMISPLDFKGTICKINSRFSSYEHQDSQELLLFLMDGLHEDLNKADKRRGYKEEEIEHLDDVSAADLAWSKHKQLNESIIVALFQGQFKSTVQCMSCQHKSRTFETFMYLTLEMTASSKCSLQDCLKLFHKEERLTDGNRFYCRHCKTHRDAIKRMQIWKLPPILLVHLKRFKYEGRWREKLQTLVDFPLDNLDLSQYVIGPKNNLKKYNLYAVSNHYGGMDGGHYTAYCKNPMKQRWFKFDDHEVSEISSSTVRSPAAYIFFYSSL

Target ORF information:

RefSeq Version	XM_009293354.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293354.2

ORF Insert Sequence:

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGGTGAAA 
GAGGAGCCCA ATAACAGCAA GAGTGTGTGC GATAAAGGCA GCATCAGCGC TGAGGCTCTG 
TTCCGGCTGA TGAAGGACTC CAGCATCAGT GTGTTGGTGA TGGACGCTCG GCCCGCGCTG 
GACTTTCAGG AGTCTCAGAT CAGCGTGCCG TCGCAAACCT GCATCAGTGT TCCAGAGGAG 
GCCATCAGCC CAGGGGTGAC GGTGAATCAG ATGGAGCTGA AGCTGCCTGC AGAGTCTCTG 
AGTGAGTGGA AGCGGCGTGG GTTTGTGGAT TATGTGGTTC TGCTGGACTG GTTCAGCTCC 
GTCTCTGACC TCAGGCTGGG CTCCACTCTC CAGAGTCTGA AGGACGCTCT CTATAAGTGG 
GACAACAGCA CTATCCTGCG CAGTGAGCCG CTGGTGTTGG ACGGAGGATA CGAGAGCTGG 
CTGCTGTTTT ACCCCATGTA CACCAGTAAC GCTAAAGTGA AACCCCCCCG ACAGCAGACG 
CCCAGCACAC TGCCACAGCT GAACTTCACG TACCCGTCTC TGGAGGAGCC CAAACCCGCG 
AGTGTGTGTG AAGAGCCAGC ATCAGCTGCA CAGATGAACG GCAAACCCCT GGAGGCAGAG 
CCGCCCGCAG AACCCGCTAC AAACACACCA CCACTCGCCC ATCCCACAAA CACACACACA 
CCACCACTTG CCCAACCTAC AACCACACAC ACACCACCAC TGGCTCCATC TGCAACCACA 
CACACACCAC CACTGGCTCC ATCTGCAACC ACACACACAC CACCGCTGGC TCCACCTGCT 
CACACTGAGG CTGCAGCACC GCCCACTGCC TCGCCCAGAC ACACACCACA GGTGGACCGC 
TCCAAGAAAC CGTCAGTGAT TCCCCCGTCC AGCTCTAAGC TCATCAGTGA TCCTGCAGTC 
AGCGAGGGCC CGGCGCCACC AGCAGCACAC AATGGTCCCT CTGTGCCCGA TCGCTCCGTC 
AAACCCACCA CAGACCCCTC CCGAGCCCTG AGCGCGGAGC AGCGGTGGGA GATCCAGAAA 
GACACGCTGG ACCTGATGGA GAAGGCCAAG AGAGAGCAGG AGCAGCGCAG GAGGGCCCAG 
GAGCAGGAGA AGCTGGAGGA CAAACAGCAG GACAGGAGGA AGCTGGAGCG GCAGACGGCC 
GAGGAGGAGG ACCTGAAGGA GACCAGCCCT GAAGACAAGA GCCGCAAAAC ACAGAAGGAC 
ACACCGGCGG ACGCCCCGAT GAAGAGCATG TCTCTGGACT CACCTGCACA GTTCAACACT 
AGGGAGCCAC TGACCCGAGC ACACAGTGAG GAGATGGGCA GGACAGTCCC GGGGCTGCCT 
GATGGCTGGA TGAAGTTTCT GGACACGGTG ACGGGCACCT ACAGATATTA CCACTCTCCC 
ACCAACCGCG TGCACCTGTA CCCTCCTGAA GTCAGCACAC CGCAGACGCC AGCCAAACAC 
ACACCGCCCG CGCAGCCGGC AGCAGACACG GAGACGGAGA AGCAGCGGCA GCGGGACCGA 
GAGAGAGAAC AGTCCAAACT CAAGCGCTCA TACTCCTCTC CAGACATCAG CCAGGAGCTC 
AGCGCAGAGA CCAGACAGAG ACCCGCTGCA GTGCCCAGCA TCAACAGAGA GAACAAGCCT 
CTAGCTGCCA GCACATATGC GAAGGTGGAG ATCACGCGTC CGTCTGCAGC AAAGATCCGC 
AACCTGAACC CAGTGTTTGG AGGCCAGGGG CCGCTGCTGA CGGGCCTCCG CAACCTGGGG 
AACACCTGCT ACATGAACTC CATCCTGCAG TGCCTGTGCA ACACCGTGGC CATGGCGGAC 
TACTTCAACA GGAACTACTA TCAGGACGAC ATTAACCGGG CGAATATCCT GGGTCATAAG 
GGCGAGGTGG CGGAGGAGTT CAGCGTGGTG ATGAAGGCGC TGTGGTTGGG TCAGTATAAG 
ATGATCAGCC CGCTGGACTT TAAGGGCACC ATCTGTAAGA TCAACAGCCG CTTCTCCAGC 
TACGAGCACC AGGACTCTCA GGAGCTGCTG CTCTTCCTGA TGGACGGCCT GCATGAGGAT 
CTCAACAAGG CGGACAAGCG CAGGGGCTAT AAGGAGGAGG AGATCGAGCA TCTGGACGAC 
GTGAGCGCGG CGGATCTGGC CTGGTCCAAA CACAAGCAGC TGAACGAGTC CATCATCGTG 
GCTCTGTTTC AGGGCCAGTT CAAGTCCACG GTTCAGTGCA TGAGCTGCCA GCACAAATCA 
CGCACCTTCG AGACCTTCAT GTACCTGACG CTGGAGATGA CGGCCAGCAG CAAGTGCTCT 
CTGCAGGACT GTCTGAAACT GTTTCATAAA GAAGAGCGTC TGACTGACGG TAACCGCTTC 
TACTGCCGCC ACTGTAAGAC CCACCGCGAC GCCATCAAGA GGATGCAGAT CTGGAAGCTG 
CCGCCCATAT TACTGGTGCA CCTGAAGCGG TTTAAATATG AGGGCCGCTG GCGGGAGAAG 
CTGCAGACGC TGGTGGATTT CCCTCTGGAT AATCTGGACC TGTCGCAGTA CGTCATCGGA 
CCCAAAAACA ACCTGAAGAA GTACAACCTG TACGCCGTGT CTAATCACTA CGGCGGAATG 
GACGGCGGCC ACTACACTGC CTACTGCAAG AACCCCATGA AGCAGCGCTG GTTCAAGTTT 
GACGATCACG AGGTCTCCGA GATCTCCAGC TCCACGGTGC GCTCGCCCGC AGCTTACATA 
TTCTTCTACT CCTCTCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp8 ( XM_009293354.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291629.1 Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa22993
Clone ID Related Accession (Same CDS sequence)	XM_009293354.2 , XM_009293354.3
Accession Version	XM_009293354.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293354.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGGTGAAA 
GAGGAGCCCA ATAACAGCAA GAGTGTGTGC GATAAAGGCA GCATCAGCGC TGAGGCTCTG 
TTCCGGCTGA TGAAGGACTC CAGCATCAGT GTGTTGGTGA TGGACGCTCG GCCCGCGCTG 
GACTTTCAGG AGTCTCAGAT CAGCGTGCCG TCGCAAACCT GCATCAGTGT TCCAGAGGAG 
GCCATCAGCC CAGGGGTGAC GGTGAATCAG ATGGAGCTGA AGCTGCCTGC AGAGTCTCTG 
AGTGAGTGGA AGCGGCGTGG GTTTGTGGAT TATGTGGTTC TGCTGGACTG GTTCAGCTCC 
GTCTCTGACC TCAGGCTGGG CTCCACTCTC CAGAGTCTGA AGGACGCTCT CTATAAGTGG 
GACAACAGCA CTATCCTGCG CAGTGAGCCG CTGGTGTTGG ACGGAGGATA CGAGAGCTGG 
CTGCTGTTTT ACCCCATGTA CACCAGTAAC GCTAAAGTGA AACCCCCCCG ACAGCAGACG 
CCCAGCACAC TGCCACAGCT GAACTTCACG TACCCGTCTC TGGAGGAGCC CAAACCCGCG 
AGTGTGTGTG AAGAGCCAGC ATCAGCTGCA CAGATGAACG GCAAACCCCT GGAGGCAGAG 
CCGCCCGCAG AACCCGCTAC AAACACACCA CCACTCGCCC ATCCCACAAA CACACACACA 
CCACCACTTG CCCAACCTAC AACCACACAC ACACCACCAC TGGCTCCATC TGCAACCACA 
CACACACCAC CACTGGCTCC ATCTGCAACC ACACACACAC CACCGCTGGC TCCACCTGCT 
CACACTGAGG CTGCAGCACC GCCCACTGCC TCGCCCAGAC ACACACCACA GGTGGACCGC 
TCCAAGAAAC CGTCAGTGAT TCCCCCGTCC AGCTCTAAGC TCATCAGTGA TCCTGCAGTC 
AGCGAGGGCC CGGCGCCACC AGCAGCACAC AATGGTCCCT CTGTGCCCGA TCGCTCCGTC 
AAACCCACCA CAGACCCCTC CCGAGCCCTG AGCGCGGAGC AGCGGTGGGA GATCCAGAAA 
GACACGCTGG ACCTGATGGA GAAGGCCAAG AGAGAGCAGG AGCAGCGCAG GAGGGCCCAG 
GAGCAGGAGA AGCTGGAGGA CAAACAGCAG GACAGGAGGA AGCTGGAGCG GCAGACGGCC 
GAGGAGGAGG ACCTGAAGGA GACCAGCCCT GAAGACAAGA GCCGCAAAAC ACAGAAGGAC 
ACACCGGCGG ACGCCCCGAT GAAGAGCATG TCTCTGGACT CACCTGCACA GTTCAACACT 
AGGGAGCCAC TGACCCGAGC ACACAGTGAG GAGATGGGCA GGACAGTCCC GGGGCTGCCT 
GATGGCTGGA TGAAGTTTCT GGACACGGTG ACGGGCACCT ACAGATATTA CCACTCTCCC 
ACCAACCGCG TGCACCTGTA CCCTCCTGAA GTCAGCACAC CGCAGACGCC AGCCAAACAC 
ACACCGCCCG CGCAGCCGGC AGCAGACACG GAGACGGAGA AGCAGCGGCA GCGGGACCGA 
GAGAGAGAAC AGTCCAAACT CAAGCGCTCA TACTCCTCTC CAGACATCAG CCAGGAGCTC 
AGCGCAGAGA CCAGACAGAG ACCCGCTGCA GTGCCCAGCA TCAACAGAGA GAACAAGCCT 
CTAGCTGCCA GCACATATGC GAAGGTGGAG ATCACGCGTC CGTCTGCAGC AAAGATCCGC 
AACCTGAACC CAGTGTTTGG AGGCCAGGGG CCGCTGCTGA CGGGCCTCCG CAACCTGGGG 
AACACCTGCT ACATGAACTC CATCCTGCAG TGCCTGTGCA ACACCGTGGC CATGGCGGAC 
TACTTCAACA GGAACTACTA TCAGGACGAC ATTAACCGGG CGAATATCCT GGGTCATAAG 
GGCGAGGTGG CGGAGGAGTT CAGCGTGGTG ATGAAGGCGC TGTGGTTGGG TCAGTATAAG 
ATGATCAGCC CGCTGGACTT TAAGGGCACC ATCTGTAAGA TCAACAGCCG CTTCTCCAGC 
TACGAGCACC AGGACTCTCA GGAGCTGCTG CTCTTCCTGA TGGACGGCCT GCATGAGGAT 
CTCAACAAGG CGGACAAGCG CAGGGGCTAT AAGGAGGAGG AGATCGAGCA TCTGGACGAC 
GTGAGCGCGG CGGATCTGGC CTGGTCCAAA CACAAGCAGC TGAACGAGTC CATCATCGTG 
GCTCTGTTTC AGGGCCAGTT CAAGTCCACG GTTCAGTGCA TGAGCTGCCA GCACAAATCA 
CGCACCTTCG AGACCTTCAT GTACCTGACG CTGGAGATGA CGGCCAGCAG CAAGTGCTCT 
CTGCAGGACT GTCTGAAACT GTTTCATAAA GAAGAGCGTC TGACTGACGG TAACCGCTTC 
TACTGCCGCC ACTGTAAGAC CCACCGCGAC GCCATCAAGA GGATGCAGAT CTGGAAGCTG 
CCGCCCATAT TACTGGTGCA CCTGAAGCGG TTTAAATATG AGGGCCGCTG GCGGGAGAAG 
CTGCAGACGC TGGTGGATTT CCCTCTGGAT AATCTGGACC TGTCGCAGTA CGTCATCGGA 
CCCAAAAACA ACCTGAAGAA GTACAACCTG TACGCCGTGT CTAATCACTA CGGCGGAATG 
GACGGCGGCC ACTACACTGC CTACTGCAAG AACCCCATGA AGCAGCGCTG GTTCAAGTTT 
GACGATCACG AGGTCTCCGA GATCTCCAGC TCCACGGTGC GCTCGCCCGC AGCTTACATA 
TTCTTCTACT CCTCTCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291629.1
CDS	188..3388
Translation	MPSVSSGLKDLYLSTSLGDLNKKAEIKPDKSSTRHYVQSACKIFKAAEECRLDRDEERAYVLYMKYLSVYDLIKKRPDFKQQQEFFLSMLGPTSFKKAIEEAEKLSESLKLRYEEVEVRKKLEEKERKEEKSRREERTEKKDGRASPQANTERKDSKKVKEEPNNSKSVCDKGSISAEALFRLMKDSSISVLVMDARPALDFQESQISVPSQTCISVPEEAISPGVTVNQMELKLPAESLSEWKRRGFVDYVVLLDWFSSVSDLRLGSTLQSLKDALYKWDNSTILRSEPLVLDGGYESWLLFYPMYTSNAKVKPPRQQTPSTLPQLNFTYPSLEEPKPASVCEEPASAAQMNGKPLEAEPPAEPATNTPPLAHPTNTHTPPLAQPTTTHTPPLAPSATTHTPPLAPSATTHTPPLAPPAHTEAAAPPTASPRHTPQVDRSKKPSVIPPSSSKLISDPAVSEGPAPPAAHNGPSVPDRSVKPTTDPSRALSAEQRWEIQKDTLDLMEKAKREQEQRRRAQEQEKLEDKQQDRRKLERQTAEEEDLKETSPEDKSRKTQKDTPADAPMKSMSLDSPAQFNTREPLTRAHSEEMGRTVPGLPDGWMKFLDTVTGTYRYYHSPTNRVHLYPPEVSTPQTPAKHTPPAQPAADTETEKQRQRDREREQSKLKRSYSSPDISQELSAETRQRPAAVPSINRENKPLAASTYAKVEITRPSAAKIRNLNPVFGGQGPLLTGLRNLGNTCYMNSILQCLCNTVAMADYFNRNYYQDDINRANILGHKGEVAEEFSVVMKALWLGQYKMISPLDFKGTICKINSRFSSYEHQDSQELLLFLMDGLHEDLNKADKRRGYKEEEIEHLDDVSAADLAWSKHKQLNESIIVALFQGQFKSTVQCMSCQHKSRTFETFMYLTLEMTASSKCSLQDCLKLFHKEERLTDGNRFYCRHCKTHRDAIKRMQIWKLPPILLVHLKRFKYEGRWREKLQTLVDFPLDNLDLSQYVIGPKNNLKKYNLYAVSNHYGGMDGGHYTAYCKNPMKQRWFKFDDHEVSEISSSTVRSPAAYIFFYSSL

Target ORF information:

RefSeq Version	XM_009293354.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 8 (usp8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293354.3

ORF Insert Sequence:

ATGCCGTCCG TGTCCAGCGG ATTGAAGGAT CTCTATCTGT CCACGTCACT CGGAGATCTG 
AACAAGAAGG CAGAAATCAA GCCGGACAAG AGCAGCACCA GACATTATGT CCAGAGCGCA 
TGTAAGATCT TCAAGGCGGC AGAGGAGTGT CGTCTGGACC GTGATGAGGA GAGGGCGTAT 
GTGCTGTACA TGAAGTATCT GAGCGTCTAT GACCTGATCA AGAAGAGGCC GGACTTCAAG 
CAGCAGCAGG AGTTTTTCCT GTCTATGCTC GGCCCGACCA GCTTCAAAAA GGCCATCGAG 
GAGGCAGAGA AACTCTCAGA GAGTCTGAAG CTGCGGTATG AGGAGGTGGA GGTGCGGAAG 
AAGCTGGAGG AGAAAGAGAG AAAAGAGGAA AAGAGTCGGA GAGAGGAGCG AACGGAGAAG 
AAGGATGGAC GCGCGTCACC TCAGGCTAAC ACTGAGAGGA AGGACAGTAA GAAGGTGAAA 
GAGGAGCCCA ATAACAGCAA GAGTGTGTGC GATAAAGGCA GCATCAGCGC TGAGGCTCTG 
TTCCGGCTGA TGAAGGACTC CAGCATCAGT GTGTTGGTGA TGGACGCTCG GCCCGCGCTG 
GACTTTCAGG AGTCTCAGAT CAGCGTGCCG TCGCAAACCT GCATCAGTGT TCCAGAGGAG 
GCCATCAGCC CAGGGGTGAC GGTGAATCAG ATGGAGCTGA AGCTGCCTGC AGAGTCTCTG 
AGTGAGTGGA AGCGGCGTGG GTTTGTGGAT TATGTGGTTC TGCTGGACTG GTTCAGCTCC 
GTCTCTGACC TCAGGCTGGG CTCCACTCTC CAGAGTCTGA AGGACGCTCT CTATAAGTGG 
GACAACAGCA CTATCCTGCG CAGTGAGCCG CTGGTGTTGG ACGGAGGATA CGAGAGCTGG 
CTGCTGTTTT ACCCCATGTA CACCAGTAAC GCTAAAGTGA AACCCCCCCG ACAGCAGACG 
CCCAGCACAC TGCCACAGCT GAACTTCACG TACCCGTCTC TGGAGGAGCC CAAACCCGCG 
AGTGTGTGTG AAGAGCCAGC ATCAGCTGCA CAGATGAACG GCAAACCCCT GGAGGCAGAG 
CCGCCCGCAG AACCCGCTAC AAACACACCA CCACTCGCCC ATCCCACAAA CACACACACA 
CCACCACTTG CCCAACCTAC AACCACACAC ACACCACCAC TGGCTCCATC TGCAACCACA 
CACACACCAC CACTGGCTCC ATCTGCAACC ACACACACAC CACCGCTGGC TCCACCTGCT 
CACACTGAGG CTGCAGCACC GCCCACTGCC TCGCCCAGAC ACACACCACA GGTGGACCGC 
TCCAAGAAAC CGTCAGTGAT TCCCCCGTCC AGCTCTAAGC TCATCAGTGA TCCTGCAGTC 
AGCGAGGGCC CGGCGCCACC AGCAGCACAC AATGGTCCCT CTGTGCCCGA TCGCTCCGTC 
AAACCCACCA CAGACCCCTC CCGAGCCCTG AGCGCGGAGC AGCGGTGGGA GATCCAGAAA 
GACACGCTGG ACCTGATGGA GAAGGCCAAG AGAGAGCAGG AGCAGCGCAG GAGGGCCCAG 
GAGCAGGAGA AGCTGGAGGA CAAACAGCAG GACAGGAGGA AGCTGGAGCG GCAGACGGCC 
GAGGAGGAGG ACCTGAAGGA GACCAGCCCT GAAGACAAGA GCCGCAAAAC ACAGAAGGAC 
ACACCGGCGG ACGCCCCGAT GAAGAGCATG TCTCTGGACT CACCTGCACA GTTCAACACT 
AGGGAGCCAC TGACCCGAGC ACACAGTGAG GAGATGGGCA GGACAGTCCC GGGGCTGCCT 
GATGGCTGGA TGAAGTTTCT GGACACGGTG ACGGGCACCT ACAGATATTA CCACTCTCCC 
ACCAACCGCG TGCACCTGTA CCCTCCTGAA GTCAGCACAC CGCAGACGCC AGCCAAACAC 
ACACCGCCCG CGCAGCCGGC AGCAGACACG GAGACGGAGA AGCAGCGGCA GCGGGACCGA 
GAGAGAGAAC AGTCCAAACT CAAGCGCTCA TACTCCTCTC CAGACATCAG CCAGGAGCTC 
AGCGCAGAGA CCAGACAGAG ACCCGCTGCA GTGCCCAGCA TCAACAGAGA GAACAAGCCT 
CTAGCTGCCA GCACATATGC GAAGGTGGAG ATCACGCGTC CGTCTGCAGC AAAGATCCGC 
AACCTGAACC CAGTGTTTGG AGGCCAGGGG CCGCTGCTGA CGGGCCTCCG CAACCTGGGG 
AACACCTGCT ACATGAACTC CATCCTGCAG TGCCTGTGCA ACACCGTGGC CATGGCGGAC 
TACTTCAACA GGAACTACTA TCAGGACGAC ATTAACCGGG CGAATATCCT GGGTCATAAG 
GGCGAGGTGG CGGAGGAGTT CAGCGTGGTG ATGAAGGCGC TGTGGTTGGG TCAGTATAAG 
ATGATCAGCC CGCTGGACTT TAAGGGCACC ATCTGTAAGA TCAACAGCCG CTTCTCCAGC 
TACGAGCACC AGGACTCTCA GGAGCTGCTG CTCTTCCTGA TGGACGGCCT GCATGAGGAT 
CTCAACAAGG CGGACAAGCG CAGGGGCTAT AAGGAGGAGG AGATCGAGCA TCTGGACGAC 
GTGAGCGCGG CGGATCTGGC CTGGTCCAAA CACAAGCAGC TGAACGAGTC CATCATCGTG 
GCTCTGTTTC AGGGCCAGTT CAAGTCCACG GTTCAGTGCA TGAGCTGCCA GCACAAATCA 
CGCACCTTCG AGACCTTCAT GTACCTGACG CTGGAGATGA CGGCCAGCAG CAAGTGCTCT 
CTGCAGGACT GTCTGAAACT GTTTCATAAA GAAGAGCGTC TGACTGACGG TAACCGCTTC 
TACTGCCGCC ACTGTAAGAC CCACCGCGAC GCCATCAAGA GGATGCAGAT CTGGAAGCTG 
CCGCCCATAT TACTGGTGCA CCTGAAGCGG TTTAAATATG AGGGCCGCTG GCGGGAGAAG 
CTGCAGACGC TGGTGGATTT CCCTCTGGAT AATCTGGACC TGTCGCAGTA CGTCATCGGA 
CCCAAAAACA ACCTGAAGAA GTACAACCTG TACGCCGTGT CTAATCACTA CGGCGGAATG 
GACGGCGGCC ACTACACTGC CTACTGCAAG AACCCCATGA AGCAGCGCTG GTTCAAGTTT 
GACGATCACG AGGTCTCCGA GATCTCCAGC TCCACGGTGC GCTCGCCCGC AGCTTACATA 
TTCTTCTACT CCTCTCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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usp8 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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