c3a.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	c3a.3
Entrez Gene ID	565074
Full Name	complement component c3a, duplicate 3
Synonyms	C3-3,c3-2,c3c,unp301,zgc:103610
General protein information	Preferred Names complement component c3a, duplicate 3 Names complement component c3a, duplicate 3 c3.3 complement component C3-2 complement component c3c
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021475507.1	XP_021331182.1	complement component c3a, duplicate 3 isoform X1
NM_001037236.2	NP_001032313.2	complement component c3a, duplicate 3 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	c3c	XP_005168460.1
Danio rerio (zebrafish)	c3a	NP_571317.1
Xenopus tropicalis (tropical clawed frog)	c3	XP_004919147.1
Danio rerio (zebrafish)	LOC100331492	XP_002660624.3
Xenopus tropicalis (tropical clawed frog)	LOC100492293	XP_002940095.2
Xenopus tropicalis (tropical clawed frog)	LOC100497914	XP_002941061.2
Canis lupus familiaris (dog)	C3	XP_005633285.1
Bos taurus (cattle)	C3	NP_001035559.2
Mus musculus (house mouse)	C3	NP_033908.2
Rattus norvegicus (Norway rat)	C3	NP_058690.2
Xenopus tropicalis (tropical clawed frog)	LOC100492966	XP_002940099.2
Homo sapiens (human)	C3	NP_000055.2
Gallus gallus (chicken)	C3	NP_990736.1
Danio rerio (zebrafish)	LOC100331428	XP_002660623.3
Xenopus tropicalis (tropical clawed frog)	LOC100491968	XP_004919148.1
Pan troglodytes (chimpanzee)	C3	XP_512318.4
Macaca mulatta (Rhesus monkey)	C3	XP_001091921.2
Danio rerio (zebrafish)	zgc:103710	NP_001008582.3
Danio rerio (zebrafish)	c3b	NP_571318.1
Xenopus tropicalis (tropical clawed frog)	LOC100492134	XP_002940094.2
Xenopus tropicalis (tropical clawed frog)	LOC100497762	XP_004919229.1

Gene Ontology
Bibliography

Related articles in PubMed

Notch1a can widely mediate innate immune responses in zebrafish larvae infected with Vibrio parahaemolyticus.
Ji C, Guo X, Dong X, Ren J, Zu Y, Li W, Zhang Q
Fish & shellfish immunology92680-689(2019 Sep)

Comparative study of stress and immune-related transcript outcomes triggered by Vibrio anguillarum bacterin and air exposure stress in liver and spleen of gilthead seabream (Sparus aurata), zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss).
Khansari AR, Balasch JC, Vallejos-Vidal E, Teles M, Fierro-Castro C, Tort L, Reyes-López FE
Fish & shellfish immunology86436-448(2019 Mar)

Differential transcriptome analysis of zebrafish (Danio rerio) larvae challenged by Vibrio parahaemolyticus.
Zhang Q, Ji C, Ren J, Zhang Q, Dong X, Zu Y, Jia L, Li W
Journal of fish diseases41(7)1049-1062(2018 Jul)

Deletion of mstna and mstnb impairs the immune system and affects growth performance in zebrafish.
Wang C, Chen YL, Bian WP, Xie SL, Qi GL, Liu L, Strauss PR, Zou JX, Pei DS
Fish & shellfish immunology72572-580(2018 Jan)

Association between adaptive immunity and neutrophil dynamics in zebrafish (Danio rerio) infected by a parasitic ciliate.
Jørgensen LVG, Korbut R, Jeberg S, Kania PW, Buchmann K
PloS one13(9)e0203297(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following c3a.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the c3a.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa39240	NM_001037236.2 Latest version!	Danio rerio complement component c3a, duplicate 3 (c3a.3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa147274	XM_021475507.1 Latest version!	Danio rerio complement component c3a, duplicate 3 (c3a.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

c3a.3 ( NM_001037236.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001032313.2 Danio rerio complement component c3a, duplicate 3 (c3a.3), mRNA.

Price & Availability↑

CloneID	ODa39240
Clone ID Related Accession (Same CDS sequence)	NM_001037236.2
Accession Version	NM_001037236.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1578067200000
Organism	Danio rerio(zebrafish)
Product	complement component c3a, duplicate 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL928676.13. On Jun 20, 2016 this sequence version replaced NM_001037236.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB235998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505377 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGTGA AGCTGCTGTT TCTCTCTGCT GTTCTTCTCT CCTCAGCGCT CCTCACACTG 
TGTGACCCGC TATATGTGCT GTCGGCTCCT AATCTGCTGA GAGTGGGTTC CTCTGAGAAC 
GTGTTTGTGG AGGCGCAGGA TTATTCTGGA GGACCCCTTA ATGTGAGAAT CTCTGTGAAG 
AACTTCCCAG CCAAGAATGT GGAGATGATG CAAAAAACAG TGACTCTAAC CGGAGCAAAA 
TACCAGAGTC TTACAGATAT ACAGATCCCT GGTGATAGAA ACTACTTCTC TGATGATGAG 
AAGAAGCAGT ATGTGTATCT GCAAGCTCAC TTTCCCTCCG TGACTCTGGA GAAAGTGGTC 
ATGGTGTCTT TTCAGTCTGG ATATTTGTTT GTTCAGACGG ACAAGCCCAT CTACACGCCT 
GGCAGCAATG TTCATTACAG GATTTTTTCT ATGACACCCA ACTTGAAACC ATTCAGTAAG 
CCCGGAATCA CAGTGGATAT CACGAATCCA CAAGGCATCA CGGTAGCATC AGACACGATA 
TTTGCAGAAA AAGGTGTGAA ATCTTCGATC TACAACATCC CTGAGGTTAC TAGTTTGGGG 
ATGTGGAAGG TGGTCACGCG GTATGCAAAC ACCCCTCAGA AGACGTTTAC TGCAGAGTTT 
GAGGTTAAAG AATACGTTCT TCCAACATTT GAAGTGAAAT TAAAAACCAG TAAATCTTTT 
TACTATGTGG GAGACAGCAA ACCAAAGAAC AACGAGGACG ACAGTCTGAC AGTTGACATG 
GAAGCCAAGT ATCTGTTTGG AAAGAAGATG GATGGAAATG CGTTTGTAGT GTTTGGTGTG 
ATGGACGGCG CGATGAAAAC AAGTATTGTG AACTCTCTTC AAAAAGTTCA GGTTTTCAAT 
GGAGTAGGAA CTGCAGAATT GACCAGGGAG ATGATCACGA AGACATTTAA AAACATAAAA 
GATCTGGTTG GGAAGTCAAT CTACGTTTCA GTCAACCTTT TAACCGAAAG TGGCAGTGAA 
ATGGTGGAAG CAGAAAAGAG AGGCATTCAG ATTGTGACGT CTCCGTACAC CATCCATTAC 
AAAAGAACAT CACAGTTCTT CAAACCTGGA ATGCCCCTGG GTGTCTCGGT TTATGTTACA 
AATCCTGATG AGACGCCTGC AGAAGATGTG GAAGTGGAGG TGCTGGTTGA TGGAGTAGGA 
GGAGTCAACA GTAAAACCAA AGCCAATGGC ATTGCGAAAG TGACAGTCAA CACTAAGGCA 
GGAGTCTCTA CATTGAAGAT CACTGCAACG ACCAGAGATC CTGAATTGGC CAATGAGCAG 
CAGGCGGATA AGACAATGAT TGCTCAGGCC TACAAAACCA ATCTTGGCTC CAAAAATTAC 
CTGCATATCA ACATTGAGTC AGCAGAGTTT GAGATTGGTG ATCAAATGAC AGTTTATCTG 
AACACTGGAG AAAGTCCAGG AGTCAAAGAT CAAGATTTCA CTTACATGAT CTTGAGTAAA 
GGTCAGATTG TTCTAGCAGA AAGGTTTAAG AGGCAAGGAC AAACTCTAGT CTCTCTGTCT 
CTACCTGTGA CCAAAGACAT GGTGCCGTCC TTCCGCTTCG TGGCTTATTA CCATGTGGGC 
AAAGATGAGG TGGTGTCTGA TTCAGTCTGG GTCGATGTGA AGGACACATG CATGGGAAAG 
CTCAAGGTCG AGGTGACAGA AAAGCAGAAA TTCTATGAAA CAGGAAATGA AGTCAATCTG 
CAAATAAGTG GAGATTTTGG AGCCCGAGTT GGTCTGGTGG TGGTTGACAA GGCTGTGCAA 
GTCCTTAACA AGAACAGACT CACTCAGTCG AAGATCTGGG ATGTCATTGA GAAGCATGAT 
ACTGGCTGTA CGGGAGGAGG TGGAAAAGAC AGTATGGGGG TTTTCAGTGA CGCTGGTTTG 
ATGTTTGAGT CCGACCCTGC AGGAGGAACC AATACAAGAA CAGAGCCCAA GTGTCCTGCC 
CCTCCAAAGA GAAAACGGAG ATCTGAGAGT CTTCAGACAA TCACTACAAC TCTCGCTGGC 
CAATACACTG AAAAAATCCG GCCATGCTGC TATGACGGGA TGAGGAATAA CAGAATTGGC 
TACACATGTG AACGGCGAGC ATCATACGTC ATAGATGGAG AAGAGTGTGT CAAGGCCTTC 
TTGCACTGCT GCAAAGAGGT GAAAAACCAC AAAGAGACTG AAGAGGAGGA GCTGATTCTG 
GCTCGTAAAG ATGATGAAGA GACTTATGAA GAGGATTATG AAGACTATGA TGACATTACA 
TCACGCAAGC AGTTTCCTGA GAGCTGGCTT TGGGAGGAGT TTGATCTCTG TGACAAATGT 
TCAAAACCAA GCAAGAACAA AGTGACGTAC CTGAAAGACT CTATTACTAC CTGGCAGATC 
TTGGCAATCA GCCTCTCACC AACACACGGC ATCTGTGTGG CAGAACCTGA GGAGATCGTC 
GTTTTTAAGA GTTTTTTCAT TGACCTCAAA GTGCCTTACT CAGCTGTACG AGGTGAACAA 
GTGGAAATCA AGGCCATTAT TCACAACTAC ACCCCCAAAC ACCTGAAGAA GGTTCGTGTG 
GAGTTTTTGG AGACAGCAGG TGTTTGCAGT GCTGCCAGTA AGAAAGGGAA ATACAGAGCA 
ACAGTAAATG TTGACAAGGA CTCCAGCATA GCAGTTTCAT TTGTGATCGT TCCAATGACA 
CTGGAAAGTC AGCATATTGA GGTGAAAGCT TCAATATTAG ATTATACAGA TGGAGTTAGA 
AAGACCCTGA AGGTGGTGTC TGAAGGTGTG CTGACTAAAT TCCAAAGCCA AAAACTGGAG 
CTCAATCCGG CCAAAAAAGG GGAGCAGCCC TTACTTCTTA GAGCTGGTAT ACCAGGAGAT 
CAGGTTCCAG GCACACCCGT TCACTCGTAC ATCACAGTTA CTGGTGAAGA AATCAGTCAG 
TCGATTGAGC ATGCCATCAA TGGAAGCTTC ATGGGAGAAC TTATCAAACG GCCAGGAGGA 
TGTGGAGAGC AGAACATGGC TAAAATGACT TTGCCTGTTG TTGCTACTCA TTATCTGGAC 
AACACCAATC AGTGGGAATC TGTTGGCATT GACCGCCGTA AAGTTGCCAT TAACCATATC 
AAAACAGGTT ATGATGGGCA GCTAAAGTAC CGTAAACCTG ATGGATCTTA CGCTATCTGG 
ATTAACACGC CGAGTAGTAC ATGGCTGACA GCATATGTGG CTAAAGTCTT CGCCATGGCC 
AATGATTTTT TTGCTATAGA AGAAAATGTG ATCTGCAGCG CCCTGAAGTG GCTGGTTATG 
CACAAACAAC TACCAGATGG CTCTTTTAAA GAGGATGCAC CTGTATATGA CAAAAGTATA 
ATGGGTGGGA TAGAAGGCCA AAACACTGAT GTCTCTCTGA CAGCATTTGT CTTGATTGCC 
ATGCAAGAGG GCAAAGAGAT CTGTGGGGGA ACAGTTGGTA GTCTGCATGA AAGCATGAGG 
AAGGCTGTTG CTTTCTTGAA AGATCAGCTT CCAAAACTGA CCAATCCTTA TGCAGTTGCT 
TTGACGTCCT ACGCCCTGGC CAACGCTGAC AGTCTTAATA AAGCAATCTT GATGATGCAT 
TCCACTAAAG TAAACGATGA AAGGTTCTGG AATGTCCCTG AACGGCAATA TTACTCAATA 
GAGGCGACGG CCTATGCTGT GCTGGCACTC GTGAAGGCCA AAGACTATGA TACAGCAGGA 
GAGGCAGTGC ATTGGCTGGC CAAACAAAAG TCTAGTCATG GTGGTTATGA CACTACACAG 
GTGACCATCA TGGTTTTCCA GGCTGTGGCA GAGTACCATG CGCAAACGCA AGACTTAAAT 
AACTTTGACC TGAATGTGGA GCTGGTTGTG CCAGGAAGAA GAAAAACTGA CAGATGGACC 
ATTCAAAGAA GTAATGTGCA TCTTGCGCGG TCAGGCAATG TGGATTTGAA TAAGGACTTC 
ACTGTGATTG CTAAAGGAAC CGGATCAGCC ATTCTCTCAG TTCTGACGCT GTACTACGCC 
AGACCGTTTG AGAAGAAGAC TGACTGTACT TTCTTTGATC TGAGTGTCAA ATTGGATAAA 
ATCCCTGAAA CAAAACCAGG AACCAATGGA ACGTACAACT TCATCCTGGA TTTCTTCTAC 
AAAAACAAAA AAAATGACGC CACCATGACG ATTCTGGACA TTGGCTTGCC AACAGGATTT 
GATATCGATG AAGCTGATCT TAAACAGTTG TCCACAGGGA AGGAGAGATA CATTCAGAAA 
TATGAAAAGA ATAAAGTGCT CTCAGAGCGA GGATCCCTCA TCATCTACTT AAACAAGGTT 
TCTCATGAAG TATCAGAAAA ACTTGTCTTC AGAATGCACA AGACCTTTAA TGTGGCTTTG 
CTTCAACCTG CTTCTGTCAC CATATACGAG TATTACTCAC CCGATGCCCG CTGTAAAAAG 
TACTACCACC CTGAAAGAGA AGATTCCGCC CTATACAGAC TGTGTAAAGG AGACCTGTGT 
GTGTGTGCTG AAGAAAACTG CAGTTACCAA AAGAAAAACC ATGTTGATGA ATCTAAGCGC 
TTGATTAAAG CCTGTGAACC CCATCTGGAT TACATTTATA AAGCGAAATT AGTGAAGATG 
GACCTGAAAC AAGACTCCGA TATCTATCAC ATGAAGGTGG AGAAGACGGT GAAAGAAGGA 
ACTGATACGG ATGTTGAAGG GAAAGTGAAA CAATTTTTGG CTCGTCCCAG TTGCCGAGTG 
AGTTTAGGAC TGGTTGAAGG AAAGTCGTAT CTCATCATGG GAAACTCTGT TGATCTGCCG 
ACCCTTGGGG GAAGTCTGCA GTACGTCATA GGAGAGCAGA CCTGGATTGA ATACTGGCCT 
ACAAGTGATG AAAGTAAATC TGCTGAACAC AGAGATCGCT ACATCGGCAT GACTGCAGTC 
GAGAATATGT TTACCACAGG AGGATGTTTG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032313.2
CDS	38..4993
Translation	MEVKLLFLSAVLLSSALLTLCDPLYVLSAPNLLRVGSSENVFVEAQDYSGGPLNVRISVKNFPAKNVEMMQKTVTLTGAKYQSLTDIQIPGDRNYFSDDEKKQYVYLQAHFPSVTLEKVVMVSFQSGYLFVQTDKPIYTPGSNVHYRIFSMTPNLKPFSKPGITVDITNPQGITVASDTIFAEKGVKSSIYNIPEVTSLGMWKVVTRYANTPQKTFTAEFEVKEYVLPTFEVKLKTSKSFYYVGDSKPKNNEDDSLTVDMEAKYLFGKKMDGNAFVVFGVMDGAMKTSIVNSLQKVQVFNGVGTAELTREMITKTFKNIKDLVGKSIYVSVNLLTESGSEMVEAEKRGIQIVTSPYTIHYKRTSQFFKPGMPLGVSVYVTNPDETPAEDVEVEVLVDGVGGVNSKTKANGIAKVTVNTKAGVSTLKITATTRDPELANEQQADKTMIAQAYKTNLGSKNYLHINIESAEFEIGDQMTVYLNTGESPGVKDQDFTYMILSKGQIVLAERFKRQGQTLVSLSLPVTKDMVPSFRFVAYYHVGKDEVVSDSVWVDVKDTCMGKLKVEVTEKQKFYETGNEVNLQISGDFGARVGLVVVDKAVQVLNKNRLTQSKIWDVIEKHDTGCTGGGGKDSMGVFSDAGLMFESDPAGGTNTRTEPKCPAPPKRKRRSESLQTITTTLAGQYTEKIRPCCYDGMRNNRIGYTCERRASYVIDGEECVKAFLHCCKEVKNHKETEEEELILARKDDEETYEEDYEDYDDITSRKQFPESWLWEEFDLCDKCSKPSKNKVTYLKDSITTWQILAISLSPTHGICVAEPEEIVVFKSFFIDLKVPYSAVRGEQVEIKAIIHNYTPKHLKKVRVEFLETAGVCSAASKKGKYRATVNVDKDSSIAVSFVIVPMTLESQHIEVKASILDYTDGVRKTLKVVSEGVLTKFQSQKLELNPAKKGEQPLLLRAGIPGDQVPGTPVHSYITVTGEEISQSIEHAINGSFMGELIKRPGGCGEQNMAKMTLPVVATHYLDNTNQWESVGIDRRKVAINHIKTGYDGQLKYRKPDGSYAIWINTPSSTWLTAYVAKVFAMANDFFAIEENVICSALKWLVMHKQLPDGSFKEDAPVYDKSIMGGIEGQNTDVSLTAFVLIAMQEGKEICGGTVGSLHESMRKAVAFLKDQLPKLTNPYAVALTSYALANADSLNKAILMMHSTKVNDERFWNVPERQYYSIEATAYAVLALVKAKDYDTAGEAVHWLAKQKSSHGGYDTTQVTIMVFQAVAEYHAQTQDLNNFDLNVELVVPGRRKTDRWTIQRSNVHLARSGNVDLNKDFTVIAKGTGSAILSVLTLYYARPFEKKTDCTFFDLSVKLDKIPETKPGTNGTYNFILDFFYKNKKNDATMTILDIGLPTGFDIDEADLKQLSTGKERYIQKYEKNKVLSERGSLIIYLNKVSHEVSEKLVFRMHKTFNVALLQPASVTIYEYYSPDARCKKYYHPEREDSALYRLCKGDLCVCAEENCSYQKKNHVDESKRLIKACEPHLDYIYKAKLVKMDLKQDSDIYHMKVEKTVKEGTDTDVEGKVKQFLARPSCRVSLGLVEGKSYLIMGNSVDLPTLGGSLQYVIGEQTWIEYWPTSDESKSAEHRDRYIGMTAVENMFTTGGCLT

Target ORF information:

RefSeq Version	NM_001037236.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement component c3a, duplicate 3 (c3a.3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037236.2

ORF Insert Sequence:

ATGGAGGTGA AGCTGCTGTT TCTCTCTGCT GTTCTTCTCT CCTCAGCGCT CCTCACACTG 
TGTGACCCGC TATATGTGCT GTCGGCTCCT AATCTGCTGA GAGTGGGTTC CTCTGAGAAC 
GTGTTTGTGG AGGCGCAGGA TTATTCTGGA GGACCCCTTA ATGTGAGAAT CTCTGTGAAG 
AACTTCCCAG CCAAGAATGT GGAGATGATG CAAAAAACAG TGACTCTAAC CGGAGCAAAA 
TACCAGAGTC TTACAGATAT ACAGATCCCT GGTGATAGAA ACTACTTCTC TGATGATGAG 
AAGAAGCAGT ATGTGTATCT GCAAGCTCAC TTTCCCTCCG TGACTCTGGA GAAAGTGGTC 
ATGGTGTCTT TTCAGTCTGG ATATTTGTTT GTTCAGACGG ACAAGCCCAT CTACACGCCT 
GGCAGCAATG TTCATTACAG GATTTTTTCT ATGACACCCA ACTTGAAACC ATTCAGTAAG 
CCCGGAATCA CAGTGGATAT CACGAATCCA CAAGGCATCA CGGTAGCATC AGACACGATA 
TTTGCAGAAA AAGGTGTGAA ATCTTCGATC TACAACATCC CTGAGGTTAC TAGTTTGGGG 
ATGTGGAAGG TGGTCACGCG GTATGCAAAC ACCCCTCAGA AGACGTTTAC TGCAGAGTTT 
GAGGTTAAAG AATACGTTCT TCCAACATTT GAAGTGAAAT TAAAAACCAG TAAATCTTTT 
TACTATGTGG GAGACAGCAA ACCAAAGAAC AACGAGGACG ACAGTCTGAC AGTTGACATG 
GAAGCCAAGT ATCTGTTTGG AAAGAAGATG GATGGAAATG CGTTTGTAGT GTTTGGTGTG 
ATGGACGGCG CGATGAAAAC AAGTATTGTG AACTCTCTTC AAAAAGTTCA GGTTTTCAAT 
GGAGTAGGAA CTGCAGAATT GACCAGGGAG ATGATCACGA AGACATTTAA AAACATAAAA 
GATCTGGTTG GGAAGTCAAT CTACGTTTCA GTCAACCTTT TAACCGAAAG TGGCAGTGAA 
ATGGTGGAAG CAGAAAAGAG AGGCATTCAG ATTGTGACGT CTCCGTACAC CATCCATTAC 
AAAAGAACAT CACAGTTCTT CAAACCTGGA ATGCCCCTGG GTGTCTCGGT TTATGTTACA 
AATCCTGATG AGACGCCTGC AGAAGATGTG GAAGTGGAGG TGCTGGTTGA TGGAGTAGGA 
GGAGTCAACA GTAAAACCAA AGCCAATGGC ATTGCGAAAG TGACAGTCAA CACTAAGGCA 
GGAGTCTCTA CATTGAAGAT CACTGCAACG ACCAGAGATC CTGAATTGGC CAATGAGCAG 
CAGGCGGATA AGACAATGAT TGCTCAGGCC TACAAAACCA ATCTTGGCTC CAAAAATTAC 
CTGCATATCA ACATTGAGTC AGCAGAGTTT GAGATTGGTG ATCAAATGAC AGTTTATCTG 
AACACTGGAG AAAGTCCAGG AGTCAAAGAT CAAGATTTCA CTTACATGAT CTTGAGTAAA 
GGTCAGATTG TTCTAGCAGA AAGGTTTAAG AGGCAAGGAC AAACTCTAGT CTCTCTGTCT 
CTACCTGTGA CCAAAGACAT GGTGCCGTCC TTCCGCTTCG TGGCTTATTA CCATGTGGGC 
AAAGATGAGG TGGTGTCTGA TTCAGTCTGG GTCGATGTGA AGGACACATG CATGGGAAAG 
CTCAAGGTCG AGGTGACAGA AAAGCAGAAA TTCTATGAAA CAGGAAATGA AGTCAATCTG 
CAAATAAGTG GAGATTTTGG AGCCCGAGTT GGTCTGGTGG TGGTTGACAA GGCTGTGCAA 
GTCCTTAACA AGAACAGACT CACTCAGTCG AAGATCTGGG ATGTCATTGA GAAGCATGAT 
ACTGGCTGTA CGGGAGGAGG TGGAAAAGAC AGTATGGGGG TTTTCAGTGA CGCTGGTTTG 
ATGTTTGAGT CCGACCCTGC AGGAGGAACC AATACAAGAA CAGAGCCCAA GTGTCCTGCC 
CCTCCAAAGA GAAAACGGAG ATCTGAGAGT CTTCAGACAA TCACTACAAC TCTCGCTGGC 
CAATACACTG AAAAAATCCG GCCATGCTGC TATGACGGGA TGAGGAATAA CAGAATTGGC 
TACACATGTG AACGGCGAGC ATCATACGTC ATAGATGGAG AAGAGTGTGT CAAGGCCTTC 
TTGCACTGCT GCAAAGAGGT GAAAAACCAC AAAGAGACTG AAGAGGAGGA GCTGATTCTG 
GCTCGTAAAG ATGATGAAGA GACTTATGAA GAGGATTATG AAGACTATGA TGACATTACA 
TCACGCAAGC AGTTTCCTGA GAGCTGGCTT TGGGAGGAGT TTGATCTCTG TGACAAATGT 
TCAAAACCAA GCAAGAACAA AGTGACGTAC CTGAAAGACT CTATTACTAC CTGGCAGATC 
TTGGCAATCA GCCTCTCACC AACACACGGC ATCTGTGTGG CAGAACCTGA GGAGATCGTC 
GTTTTTAAGA GTTTTTTCAT TGACCTCAAA GTGCCTTACT CAGCTGTACG AGGTGAACAA 
GTGGAAATCA AGGCCATTAT TCACAACTAC ACCCCCAAAC ACCTGAAGAA GGTTCGTGTG 
GAGTTTTTGG AGACAGCAGG TGTTTGCAGT GCTGCCAGTA AGAAAGGGAA ATACAGAGCA 
ACAGTAAATG TTGACAAGGA CTCCAGCATA GCAGTTTCAT TTGTGATCGT TCCAATGACA 
CTGGAAAGTC AGCATATTGA GGTGAAAGCT TCAATATTAG ATTATACAGA TGGAGTTAGA 
AAGACCCTGA AGGTGGTGTC TGAAGGTGTG CTGACTAAAT TCCAAAGCCA AAAACTGGAG 
CTCAATCCGG CCAAAAAAGG GGAGCAGCCC TTACTTCTTA GAGCTGGTAT ACCAGGAGAT 
CAGGTTCCAG GCACACCCGT TCACTCGTAC ATCACAGTTA CTGGTGAAGA AATCAGTCAG 
TCGATTGAGC ATGCCATCAA TGGAAGCTTC ATGGGAGAAC TTATCAAACG GCCAGGAGGA 
TGTGGAGAGC AGAACATGGC TAAAATGACT TTGCCTGTTG TTGCTACTCA TTATCTGGAC 
AACACCAATC AGTGGGAATC TGTTGGCATT GACCGCCGTA AAGTTGCCAT TAACCATATC 
AAAACAGGTT ATGATGGGCA GCTAAAGTAC CGTAAACCTG ATGGATCTTA CGCTATCTGG 
ATTAACACGC CGAGTAGTAC ATGGCTGACA GCATATGTGG CTAAAGTCTT CGCCATGGCC 
AATGATTTTT TTGCTATAGA AGAAAATGTG ATCTGCAGCG CCCTGAAGTG GCTGGTTATG 
CACAAACAAC TACCAGATGG CTCTTTTAAA GAGGATGCAC CTGTATATGA CAAAAGTATA 
ATGGGTGGGA TAGAAGGCCA AAACACTGAT GTCTCTCTGA CAGCATTTGT CTTGATTGCC 
ATGCAAGAGG GCAAAGAGAT CTGTGGGGGA ACAGTTGGTA GTCTGCATGA AAGCATGAGG 
AAGGCTGTTG CTTTCTTGAA AGATCAGCTT CCAAAACTGA CCAATCCTTA TGCAGTTGCT 
TTGACGTCCT ACGCCCTGGC CAACGCTGAC AGTCTTAATA AAGCAATCTT GATGATGCAT 
TCCACTAAAG TAAACGATGA AAGGTTCTGG AATGTCCCTG AACGGCAATA TTACTCAATA 
GAGGCGACGG CCTATGCTGT GCTGGCACTC GTGAAGGCCA AAGACTATGA TACAGCAGGA 
GAGGCAGTGC ATTGGCTGGC CAAACAAAAG TCTAGTCATG GTGGTTATGA CACTACACAG 
GTGACCATCA TGGTTTTCCA GGCTGTGGCA GAGTACCATG CGCAAACGCA AGACTTAAAT 
AACTTTGACC TGAATGTGGA GCTGGTTGTG CCAGGAAGAA GAAAAACTGA CAGATGGACC 
ATTCAAAGAA GTAATGTGCA TCTTGCGCGG TCAGGCAATG TGGATTTGAA TAAGGACTTC 
ACTGTGATTG CTAAAGGAAC CGGATCAGCC ATTCTCTCAG TTCTGACGCT GTACTACGCC 
AGACCGTTTG AGAAGAAGAC TGACTGTACT TTCTTTGATC TGAGTGTCAA ATTGGATAAA 
ATCCCTGAAA CAAAACCAGG AACCAATGGA ACGTACAACT TCATCCTGGA TTTCTTCTAC 
AAAAACAAAA AAAATGACGC CACCATGACG ATTCTGGACA TTGGCTTGCC AACAGGATTT 
GATATCGATG AAGCTGATCT TAAACAGTTG TCCACAGGGA AGGAGAGATA CATTCAGAAA 
TATGAAAAGA ATAAAGTGCT CTCAGAGCGA GGATCCCTCA TCATCTACTT AAACAAGGTT 
TCTCATGAAG TATCAGAAAA ACTTGTCTTC AGAATGCACA AGACCTTTAA TGTGGCTTTG 
CTTCAACCTG CTTCTGTCAC CATATACGAG TATTACTCAC CCGATGCCCG CTGTAAAAAG 
TACTACCACC CTGAAAGAGA AGATTCCGCC CTATACAGAC TGTGTAAAGG AGACCTGTGT 
GTGTGTGCTG AAGAAAACTG CAGTTACCAA AAGAAAAACC ATGTTGATGA ATCTAAGCGC 
TTGATTAAAG CCTGTGAACC CCATCTGGAT TACATTTATA AAGCGAAATT AGTGAAGATG 
GACCTGAAAC AAGACTCCGA TATCTATCAC ATGAAGGTGG AGAAGACGGT GAAAGAAGGA 
ACTGATACGG ATGTTGAAGG GAAAGTGAAA CAATTTTTGG CTCGTCCCAG TTGCCGAGTG 
AGTTTAGGAC TGGTTGAAGG AAAGTCGTAT CTCATCATGG GAAACTCTGT TGATCTGCCG 
ACCCTTGGGG GAAGTCTGCA GTACGTCATA GGAGAGCAGA CCTGGATTGA ATACTGGCCT 
ACAAGTGATG AAAGTAAATC TGCTGAACAC AGAGATCGCT ACATCGGCAT GACTGCAGTC 
GAGAATATGT TTACCACAGG AGGATGTTTG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

c3a.3 ( XM_021475507.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331182.1 Danio rerio complement component c3a, duplicate 3 (c3a.3), transcript variant X1, mRNA.

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CloneID	ODa147274
Clone ID Related Accession (Same CDS sequence)	XM_021475507.1
Accession Version	XM_021475507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	complement component c3a, duplicate 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGA AGCTGCTGTT TCTCTCTGCT GTTCTTCTCT CCTCAGCGCT CCTCACACTG 
TGTGACCCGC TATATGTGCT GTCGGCTCCT AATCTGCTGA GAGTGGGTTC CTCTGAGAAC 
GTGTTTGTGG AGGCGCAGGA TTATTCTGGA GGACCCCTTA ATGTGAGAAT CTCTGTGAAG 
AACTTCCCAG CCAAGAATGT GGAGATGATG CAAAAAACAG TGACTCTAAC CGGAGCAAAA 
TACCAGAGTC TTACAGATAT ACAGATCCCT GGTGATAGAA ACTACTTCTC TGATGATGAG 
AAGAAGCAGT ATGTGTATCT GCAAGCTCAC TTTCCCTCCG TGACTCTGGA GAAAGTGGTC 
ATGGTGTCTT TTCAGTCTGG ATATTTGTTT GTTCAGACGG ACAAGCCCAT CTACACGCCT 
GGCAGCAATG TTCATTACAG GATTTTTTCT ATGACACCCA ACTTGAAACC ATTCAGTAAG 
CCCGGAATCA CAGTGGATAT CACGAATCCA CAAGGCATCA CGGTAGCATC AGACACGATA 
TTTGCAGAAA AAGGTGTGAA ATCTTCGATC TACAACATCC CTGAGGTTAC TAGTTTGGGG 
ATGTGGAAGG TGGTCACGCG GTATGCAAAC ACCCCTCAGA AGACGTTTAC TGCAGAGTTT 
GAGGTTAAAG AATACGTTCT TCCAACATTT GAAGTGAAAT TAAAAACCAG TAAATCTTTT 
TACTATGTGG GAGACAGCAA ACCAAAGAAC AACGAGGACG ACAGTCTGAC AGTTGACATG 
GAAGCCAAGT ATCTGTTTGG AAAGAAGATG GATGGAAATG CGTTTGTAGT GTTTGGTGTG 
ATGGACGGCG CGATGAAAAC AAGTATTGTG AACTCTCTTC AAAAAGTTCA GGTTTTCAAT 
GGAGTAGGAA CTGCAGAATT GACCAGGGAG ATGATCACGA AGACATTTAA AAACATAAAA 
GATCTGGTTG GGAAGTCAAT CTACGTTTCA GTCAACCTTT TAACCGAAAG TGGCAGTGAA 
ATGGTGGAAG CAGAAAAGAG AGGCATTCAG ATTGTGACGT CTCCGTACAC CATCCATTAC 
AAAAGAACAT CACAGTTCTT CAAACCTGGA ATGCCCCTGG GTGTCTCGGT TTATGTTACA 
AATCCTGATG AGACGCCTGC AGAAGATGTG GAAGTGGAGG TGCTGGTTGA TGGAGTAGGA 
GGAGTCAACA GTAAAACCAA AGCCAATGGC ATTGCGAAAG TGACAGTCAA CACTAAGGCA 
GGAGTCTCTA CATTGAAGAT CACTGCAACG ACCAGAGATC CTGAATTGGC CAATGAGCAG 
CAGGCGGATA AGACAATGAT TGCTCAGGCC TACAAAACCA ATCTTGGCTC CAAAAATTAC 
CTGCATATCA ACATTGAGTC AGCAGAGTTT GAGATTGGTG ATCAAATGAC AGTTTATCTG 
AACACTGGAG AAAGTCCAGG AGTCAAAGAT CAAGATTTCA CTTACATGAT CTTGAGTAAA 
GGTCAGATTG TTCTAGCAGA AAGGTTTAAG AGGCAAGGAC AAACTCTAGT CTCTCTGTCT 
CTACCTGTGA CCAAAGACAT GGTGCCGTCC TTCCGCTTCG TGGCTTATTA CCATGTGGGC 
AAAGATGAGG TGGTGTCTGA TTCAGTCTGG GTCGATGTGA AGGACACATG CATGGGAAAG 
CTCAAGGTCG AGGTGACAGA AAAGCAGAAA TTCTATGAAA CAGGAAATGA AGTCAATCTG 
CAAATAAGTG GAGATTTTGG AGCCCGAGTT GGTCTGGTGG TGGTTGACAA GGCTGTGCAA 
GTCCTTAACA AGAACAGACT CACTCAGTCG AAGATCTGGG ATGTCATTGA GAAGCATGAT 
ACTGGCTGTA CGGGAGGAGG TGGAAAAGAC AGTATGGGGG TTTTCAGTGA CGCTGGTTTG 
ATGTTTGAGT CCGACCCTGC AGGAGGAACC AATACAAGAA CAGAGCCCAA GTGTCCTGCC 
CCTCCAAAGA GAAAACGGAG ATCTGAGAGT CTTCAGACAA TCACTACAAC TCTCGCTGGC 
CAATACACTG AAAAAATCCG GCCATGCTGC TATGACGGGA TGAGGAATAA CAGAATTGGC 
TACACATGTG AACGGCGAGC ATCATACGTC ATAGATGGAG AAGAGTGTGT CAAGGCCTTC 
TTGCACTGCT GCAAAGAGGT GAAAAACCAC AAAGAGACTG AAGAGGAGGA GCTGATTCTG 
GCTCGTAATG ATGAAGAGAC TTATGAAGAG GATTATGAAG ACTATGATGA CATTACATCA 
CGCAAGCAGT TTCCTGAGAG CTGGCTTTGG GAGGAGTTTG ATCTCTGTGA CAAATGTTCA 
AAACCAAGCA AGAACAAAGT GACGTACCTG AAAGACTCTA TTACTACCTG GCAGATCTTG 
GCAATCAGCC TCTCACCAAC ACACGGCATC TGTGTGGCAG AACCTGAGGA GATCGTCGTT 
TTTAAGAGTT TTTTCATTGA CCTCAAAGTG CCTTACTCAG CTGTACGAGG TGAACAAGTG 
GAAATCAAGG CCATTATTCA CAACTACACC CCCAAACACC TGAAGAAGGT TCGTGTGGAG 
TTTTTGGAGA CAGCAGGTGT TTGCAGTGCT GCCAGTAAGA AAGGGAAATA CAGAGCAACA 
GTAAATGTTG ACAAGGACTC CAGCATAGCA GTTTCATTTG TGATCGTTCC AATGACACTG 
GAAAGTCAGC ATATTGAGGT GAAAGCTTCA ATATTAGATT ATACAGATGG AGTTAGAAAG 
ACCCTGAAGG TGGTGTCTGA AGGTGTGCTG ACTAAATTCC AAAGCCAAAA ACTGGAGCTC 
AATCCGGCCA AAAAAGGGGA GCAGCCCTTA CTTCTTAGAG CTGGTATACC AGGAGATCAG 
GTTCCAGGCA CACCCGTTCA CTCGTACATC ACAGTTACTG GTGAAGAAAT CAGTCAGTCG 
ATTGAGCATG CCATCAATGG AAGCTTCATG GGAGAACTTA TCAAACGGCC AGGAGGATGT 
GGAGAGCAGA ACATGGCTAA AATGACTTTG CCTGTTGTTG CTACTCATTA TCTGGACAAC 
ACCAATCAGT GGGAATCTGT TGGCATTGAC CGCCGTAAAG TTGCCATTAA CCATATCAAA 
ACAGGTTATG ATGGGCAGCT AAAGTACCGT AAACCTGATG GATCTTACGC TATCTGGATT 
AACACGCCGA GTAGTACATG GCTGACAGCA TATGTGGCTA AAGTCTTCGC CATGGCCAAT 
GATTTTTTTG CTATAGAAGA AAATGTGATC TGCAGCGCCC TGAAGTGGCT GGTTATGCAC 
AAACAACTAC CAGATGGCTC TTTTAAAGAG GATGCACCTG TATATGACAA AAGTATAATG 
GGTGGGATAG AAGGCCAAAA CACTGATGTC TCTCTGACAG CATTTGTCTT GATTGCCATG 
CAAGAGGGCA AAGAGATCTG TGGGGGAACA GTTGGTAGTC TGCATGAAAG CATGAGGAAG 
GCTGTTGCTT TCTTGAAAGA TCAGCTTCCA AAACTGACCA ATCCTTATGC AGTTGCTTTG 
ACGTCCTACG CCCTGGCCAA CGCTGACAGT CTTAATAAAG CAATCTTGAT GATGCATTCC 
ACTAAAGTAA ACGATGAAAG GTTCTGGAAT GTCCCTGAAC GGCAATATTA CTCAATAGAG 
GCGACGGCCT ATGCTGTGCT GGCACTCGTG AAGGCCAAAG ACTATGATAC AGCAGGAGAG 
GCAGTGCATT GGCTGGCCAA ACAAAAGTCT AGTCATGGTG GTTATGACAC TACACAGGTG 
ACCATCATGG TTTTCCAGGC TGTGGCAGAG TACCATGCGC AAACGCAAGA CTTAAATAAC 
TTTGACCTGA ATGTGGAGCT GGTTGTGCCA GGAAGAAGAA AAACTGACAG ATGGACCATT 
CAAAGAAGTA ATGTGCATCT TGCGCGGTCA GGCAATGTGG ATTTGAATAA GGACTTCACT 
GTGATTGCTA AAGGAACCGG ATCAGCCATT CTCTCAGTTC TGACGCTGTA CTACGCCAGA 
CCGTTTGAGA AGAAGACTGA CTGTACTTTC TTTGATCTGA GTGTCAAATT GGATAAAATC 
CCTGAAACAA AACCAGGAAC CAATGGAACG TACAACTTCA TCCTGGATTT CTTCTACAAA 
AACAAAAAAA ATGACGCCAC CATGACGATT CTGGACATTG GCTTGCCAAC AGGATTTGAT 
ATCGATGAAG CTGATCTTAA ACAGTTGTCC ACAGGGAAGG AGAGATACAT TCAGAAATAT 
GAAAAGAATA AAGTGCTCTC AGAGCGAGGA TCCCTCATCA TCTACTTAAA CAAGGTTTCT 
CATGAAGTAT CAGAAAAACT TGTCTTCAGA ATGCACAAGA CCTTTAATGT GGCTTTGCTT 
CAACCTGCTT CTGTCACCAT ATACGAGTAT TACTCACCCG ATGCCCGCTG TAAAAAGTAC 
TACCACCCTG AAAGAGAAGA TTCCGCCCTA TACAGACTGT GTAAAGGAGA CCTGTGTGTG 
TGTGCTGAAG AAAACTGCAG TTACCAAAAG AAAAACCATG TTGATGAATC TAAGCGCTTG 
ATTAAAGCCT GTGAACCCCA TCTGGATTAC ATTTATAAAG CGAAATTAGT GAAGATGGAC 
CTGAAACAAG ACTCCGATAT CTATCACATG AAGGTGGAGA AGACGGTGAA AGAAGGAACT 
GATACGGATG TTGAAGGGAA AGTGAAACAA TTTTTGGCTC GTCCCAGTTG CCGAGTGAGT 
TTAGGACTGG TTGAAGGAAA GTCGTATCTC ATCATGGGAA ACTCTGTTGA TCTGCCGACC 
CTTGGGGGAA GTCTGCAGTA CGTCATAGGA GAGCAGACCT GGATTGAATA CTGGCCTACA 
AGTGATGAAA GTAAATCTGC TGAACACAGA GATCGCTACA TCGGCATGAC TGCAGTCGAG 
AATATGTTTA CCACAGGAGG ATGTTTGACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331182.1
CDS	66..5018
Translation	MEVKLLFLSAVLLSSALLTLCDPLYVLSAPNLLRVGSSENVFVEAQDYSGGPLNVRISVKNFPAKNVEMMQKTVTLTGAKYQSLTDIQIPGDRNYFSDDEKKQYVYLQAHFPSVTLEKVVMVSFQSGYLFVQTDKPIYTPGSNVHYRIFSMTPNLKPFSKPGITVDITNPQGITVASDTIFAEKGVKSSIYNIPEVTSLGMWKVVTRYANTPQKTFTAEFEVKEYVLPTFEVKLKTSKSFYYVGDSKPKNNEDDSLTVDMEAKYLFGKKMDGNAFVVFGVMDGAMKTSIVNSLQKVQVFNGVGTAELTREMITKTFKNIKDLVGKSIYVSVNLLTESGSEMVEAEKRGIQIVTSPYTIHYKRTSQFFKPGMPLGVSVYVTNPDETPAEDVEVEVLVDGVGGVNSKTKANGIAKVTVNTKAGVSTLKITATTRDPELANEQQADKTMIAQAYKTNLGSKNYLHINIESAEFEIGDQMTVYLNTGESPGVKDQDFTYMILSKGQIVLAERFKRQGQTLVSLSLPVTKDMVPSFRFVAYYHVGKDEVVSDSVWVDVKDTCMGKLKVEVTEKQKFYETGNEVNLQISGDFGARVGLVVVDKAVQVLNKNRLTQSKIWDVIEKHDTGCTGGGGKDSMGVFSDAGLMFESDPAGGTNTRTEPKCPAPPKRKRRSESLQTITTTLAGQYTEKIRPCCYDGMRNNRIGYTCERRASYVIDGEECVKAFLHCCKEVKNHKETEEEELILARNDEETYEEDYEDYDDITSRKQFPESWLWEEFDLCDKCSKPSKNKVTYLKDSITTWQILAISLSPTHGICVAEPEEIVVFKSFFIDLKVPYSAVRGEQVEIKAIIHNYTPKHLKKVRVEFLETAGVCSAASKKGKYRATVNVDKDSSIAVSFVIVPMTLESQHIEVKASILDYTDGVRKTLKVVSEGVLTKFQSQKLELNPAKKGEQPLLLRAGIPGDQVPGTPVHSYITVTGEEISQSIEHAINGSFMGELIKRPGGCGEQNMAKMTLPVVATHYLDNTNQWESVGIDRRKVAINHIKTGYDGQLKYRKPDGSYAIWINTPSSTWLTAYVAKVFAMANDFFAIEENVICSALKWLVMHKQLPDGSFKEDAPVYDKSIMGGIEGQNTDVSLTAFVLIAMQEGKEICGGTVGSLHESMRKAVAFLKDQLPKLTNPYAVALTSYALANADSLNKAILMMHSTKVNDERFWNVPERQYYSIEATAYAVLALVKAKDYDTAGEAVHWLAKQKSSHGGYDTTQVTIMVFQAVAEYHAQTQDLNNFDLNVELVVPGRRKTDRWTIQRSNVHLARSGNVDLNKDFTVIAKGTGSAILSVLTLYYARPFEKKTDCTFFDLSVKLDKIPETKPGTNGTYNFILDFFYKNKKNDATMTILDIGLPTGFDIDEADLKQLSTGKERYIQKYEKNKVLSERGSLIIYLNKVSHEVSEKLVFRMHKTFNVALLQPASVTIYEYYSPDARCKKYYHPEREDSALYRLCKGDLCVCAEENCSYQKKNHVDESKRLIKACEPHLDYIYKAKLVKMDLKQDSDIYHMKVEKTVKEGTDTDVEGKVKQFLARPSCRVSLGLVEGKSYLIMGNSVDLPTLGGSLQYVIGEQTWIEYWPTSDESKSAEHRDRYIGMTAVENMFTTGGCLT

Target ORF information:

RefSeq Version	XM_021475507.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement component c3a, duplicate 3 (c3a.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475507.1

ORF Insert Sequence:

ATGGAGGTGA AGCTGCTGTT TCTCTCTGCT GTTCTTCTCT CCTCAGCGCT CCTCACACTG 
TGTGACCCGC TATATGTGCT GTCGGCTCCT AATCTGCTGA GAGTGGGTTC CTCTGAGAAC 
GTGTTTGTGG AGGCGCAGGA TTATTCTGGA GGACCCCTTA ATGTGAGAAT CTCTGTGAAG 
AACTTCCCAG CCAAGAATGT GGAGATGATG CAAAAAACAG TGACTCTAAC CGGAGCAAAA 
TACCAGAGTC TTACAGATAT ACAGATCCCT GGTGATAGAA ACTACTTCTC TGATGATGAG 
AAGAAGCAGT ATGTGTATCT GCAAGCTCAC TTTCCCTCCG TGACTCTGGA GAAAGTGGTC 
ATGGTGTCTT TTCAGTCTGG ATATTTGTTT GTTCAGACGG ACAAGCCCAT CTACACGCCT 
GGCAGCAATG TTCATTACAG GATTTTTTCT ATGACACCCA ACTTGAAACC ATTCAGTAAG 
CCCGGAATCA CAGTGGATAT CACGAATCCA CAAGGCATCA CGGTAGCATC AGACACGATA 
TTTGCAGAAA AAGGTGTGAA ATCTTCGATC TACAACATCC CTGAGGTTAC TAGTTTGGGG 
ATGTGGAAGG TGGTCACGCG GTATGCAAAC ACCCCTCAGA AGACGTTTAC TGCAGAGTTT 
GAGGTTAAAG AATACGTTCT TCCAACATTT GAAGTGAAAT TAAAAACCAG TAAATCTTTT 
TACTATGTGG GAGACAGCAA ACCAAAGAAC AACGAGGACG ACAGTCTGAC AGTTGACATG 
GAAGCCAAGT ATCTGTTTGG AAAGAAGATG GATGGAAATG CGTTTGTAGT GTTTGGTGTG 
ATGGACGGCG CGATGAAAAC AAGTATTGTG AACTCTCTTC AAAAAGTTCA GGTTTTCAAT 
GGAGTAGGAA CTGCAGAATT GACCAGGGAG ATGATCACGA AGACATTTAA AAACATAAAA 
GATCTGGTTG GGAAGTCAAT CTACGTTTCA GTCAACCTTT TAACCGAAAG TGGCAGTGAA 
ATGGTGGAAG CAGAAAAGAG AGGCATTCAG ATTGTGACGT CTCCGTACAC CATCCATTAC 
AAAAGAACAT CACAGTTCTT CAAACCTGGA ATGCCCCTGG GTGTCTCGGT TTATGTTACA 
AATCCTGATG AGACGCCTGC AGAAGATGTG GAAGTGGAGG TGCTGGTTGA TGGAGTAGGA 
GGAGTCAACA GTAAAACCAA AGCCAATGGC ATTGCGAAAG TGACAGTCAA CACTAAGGCA 
GGAGTCTCTA CATTGAAGAT CACTGCAACG ACCAGAGATC CTGAATTGGC CAATGAGCAG 
CAGGCGGATA AGACAATGAT TGCTCAGGCC TACAAAACCA ATCTTGGCTC CAAAAATTAC 
CTGCATATCA ACATTGAGTC AGCAGAGTTT GAGATTGGTG ATCAAATGAC AGTTTATCTG 
AACACTGGAG AAAGTCCAGG AGTCAAAGAT CAAGATTTCA CTTACATGAT CTTGAGTAAA 
GGTCAGATTG TTCTAGCAGA AAGGTTTAAG AGGCAAGGAC AAACTCTAGT CTCTCTGTCT 
CTACCTGTGA CCAAAGACAT GGTGCCGTCC TTCCGCTTCG TGGCTTATTA CCATGTGGGC 
AAAGATGAGG TGGTGTCTGA TTCAGTCTGG GTCGATGTGA AGGACACATG CATGGGAAAG 
CTCAAGGTCG AGGTGACAGA AAAGCAGAAA TTCTATGAAA CAGGAAATGA AGTCAATCTG 
CAAATAAGTG GAGATTTTGG AGCCCGAGTT GGTCTGGTGG TGGTTGACAA GGCTGTGCAA 
GTCCTTAACA AGAACAGACT CACTCAGTCG AAGATCTGGG ATGTCATTGA GAAGCATGAT 
ACTGGCTGTA CGGGAGGAGG TGGAAAAGAC AGTATGGGGG TTTTCAGTGA CGCTGGTTTG 
ATGTTTGAGT CCGACCCTGC AGGAGGAACC AATACAAGAA CAGAGCCCAA GTGTCCTGCC 
CCTCCAAAGA GAAAACGGAG ATCTGAGAGT CTTCAGACAA TCACTACAAC TCTCGCTGGC 
CAATACACTG AAAAAATCCG GCCATGCTGC TATGACGGGA TGAGGAATAA CAGAATTGGC 
TACACATGTG AACGGCGAGC ATCATACGTC ATAGATGGAG AAGAGTGTGT CAAGGCCTTC 
TTGCACTGCT GCAAAGAGGT GAAAAACCAC AAAGAGACTG AAGAGGAGGA GCTGATTCTG 
GCTCGTAATG ATGAAGAGAC TTATGAAGAG GATTATGAAG ACTATGATGA CATTACATCA 
CGCAAGCAGT TTCCTGAGAG CTGGCTTTGG GAGGAGTTTG ATCTCTGTGA CAAATGTTCA 
AAACCAAGCA AGAACAAAGT GACGTACCTG AAAGACTCTA TTACTACCTG GCAGATCTTG 
GCAATCAGCC TCTCACCAAC ACACGGCATC TGTGTGGCAG AACCTGAGGA GATCGTCGTT 
TTTAAGAGTT TTTTCATTGA CCTCAAAGTG CCTTACTCAG CTGTACGAGG TGAACAAGTG 
GAAATCAAGG CCATTATTCA CAACTACACC CCCAAACACC TGAAGAAGGT TCGTGTGGAG 
TTTTTGGAGA CAGCAGGTGT TTGCAGTGCT GCCAGTAAGA AAGGGAAATA CAGAGCAACA 
GTAAATGTTG ACAAGGACTC CAGCATAGCA GTTTCATTTG TGATCGTTCC AATGACACTG 
GAAAGTCAGC ATATTGAGGT GAAAGCTTCA ATATTAGATT ATACAGATGG AGTTAGAAAG 
ACCCTGAAGG TGGTGTCTGA AGGTGTGCTG ACTAAATTCC AAAGCCAAAA ACTGGAGCTC 
AATCCGGCCA AAAAAGGGGA GCAGCCCTTA CTTCTTAGAG CTGGTATACC AGGAGATCAG 
GTTCCAGGCA CACCCGTTCA CTCGTACATC ACAGTTACTG GTGAAGAAAT CAGTCAGTCG 
ATTGAGCATG CCATCAATGG AAGCTTCATG GGAGAACTTA TCAAACGGCC AGGAGGATGT 
GGAGAGCAGA ACATGGCTAA AATGACTTTG CCTGTTGTTG CTACTCATTA TCTGGACAAC 
ACCAATCAGT GGGAATCTGT TGGCATTGAC CGCCGTAAAG TTGCCATTAA CCATATCAAA 
ACAGGTTATG ATGGGCAGCT AAAGTACCGT AAACCTGATG GATCTTACGC TATCTGGATT 
AACACGCCGA GTAGTACATG GCTGACAGCA TATGTGGCTA AAGTCTTCGC CATGGCCAAT 
GATTTTTTTG CTATAGAAGA AAATGTGATC TGCAGCGCCC TGAAGTGGCT GGTTATGCAC 
AAACAACTAC CAGATGGCTC TTTTAAAGAG GATGCACCTG TATATGACAA AAGTATAATG 
GGTGGGATAG AAGGCCAAAA CACTGATGTC TCTCTGACAG CATTTGTCTT GATTGCCATG 
CAAGAGGGCA AAGAGATCTG TGGGGGAACA GTTGGTAGTC TGCATGAAAG CATGAGGAAG 
GCTGTTGCTT TCTTGAAAGA TCAGCTTCCA AAACTGACCA ATCCTTATGC AGTTGCTTTG 
ACGTCCTACG CCCTGGCCAA CGCTGACAGT CTTAATAAAG CAATCTTGAT GATGCATTCC 
ACTAAAGTAA ACGATGAAAG GTTCTGGAAT GTCCCTGAAC GGCAATATTA CTCAATAGAG 
GCGACGGCCT ATGCTGTGCT GGCACTCGTG AAGGCCAAAG ACTATGATAC AGCAGGAGAG 
GCAGTGCATT GGCTGGCCAA ACAAAAGTCT AGTCATGGTG GTTATGACAC TACACAGGTG 
ACCATCATGG TTTTCCAGGC TGTGGCAGAG TACCATGCGC AAACGCAAGA CTTAAATAAC 
TTTGACCTGA ATGTGGAGCT GGTTGTGCCA GGAAGAAGAA AAACTGACAG ATGGACCATT 
CAAAGAAGTA ATGTGCATCT TGCGCGGTCA GGCAATGTGG ATTTGAATAA GGACTTCACT 
GTGATTGCTA AAGGAACCGG ATCAGCCATT CTCTCAGTTC TGACGCTGTA CTACGCCAGA 
CCGTTTGAGA AGAAGACTGA CTGTACTTTC TTTGATCTGA GTGTCAAATT GGATAAAATC 
CCTGAAACAA AACCAGGAAC CAATGGAACG TACAACTTCA TCCTGGATTT CTTCTACAAA 
AACAAAAAAA ATGACGCCAC CATGACGATT CTGGACATTG GCTTGCCAAC AGGATTTGAT 
ATCGATGAAG CTGATCTTAA ACAGTTGTCC ACAGGGAAGG AGAGATACAT TCAGAAATAT 
GAAAAGAATA AAGTGCTCTC AGAGCGAGGA TCCCTCATCA TCTACTTAAA CAAGGTTTCT 
CATGAAGTAT CAGAAAAACT TGTCTTCAGA ATGCACAAGA CCTTTAATGT GGCTTTGCTT 
CAACCTGCTT CTGTCACCAT ATACGAGTAT TACTCACCCG ATGCCCGCTG TAAAAAGTAC 
TACCACCCTG AAAGAGAAGA TTCCGCCCTA TACAGACTGT GTAAAGGAGA CCTGTGTGTG 
TGTGCTGAAG AAAACTGCAG TTACCAAAAG AAAAACCATG TTGATGAATC TAAGCGCTTG 
ATTAAAGCCT GTGAACCCCA TCTGGATTAC ATTTATAAAG CGAAATTAGT GAAGATGGAC 
CTGAAACAAG ACTCCGATAT CTATCACATG AAGGTGGAGA AGACGGTGAA AGAAGGAACT 
GATACGGATG TTGAAGGGAA AGTGAAACAA TTTTTGGCTC GTCCCAGTTG CCGAGTGAGT 
TTAGGACTGG TTGAAGGAAA GTCGTATCTC ATCATGGGAA ACTCTGTTGA TCTGCCGACC 
CTTGGGGGAA GTCTGCAGTA CGTCATAGGA GAGCAGACCT GGATTGAATA CTGGCCTACA 
AGTGATGAAA GTAAATCTGC TGAACACAGA GATCGCTACA TCGGCATGAC TGCAGTCGAG 
AATATGTTTA CCACAGGAGG ATGTTTGACA TAA