c3a.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	c3a.2
Entrez Gene ID	30491
Full Name	complement component c3a, duplicate 2
Synonyms	C3-1,C3-2,c3b
General protein information	Preferred Names complement component c3a, duplicate 2 Names complement component c3a, duplicate 2 c3.2 complement component C3-1 complement component c3b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131243.1	NP_571318.1	complement component c3a, duplicate 2 precursor
XM_021478306.1	XP_021333981.1	complement component c3a, duplicate 2 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	c3c	XP_005168460.1
Danio rerio (zebrafish)	c3a	NP_571317.1
Xenopus tropicalis (tropical clawed frog)	c3	XP_004919147.1
Danio rerio (zebrafish)	LOC100331492	XP_002660624.3
Xenopus tropicalis (tropical clawed frog)	LOC100492293	XP_002940095.2
Xenopus tropicalis (tropical clawed frog)	LOC100497914	XP_002941061.2
Canis lupus familiaris (dog)	C3	XP_005633285.1
Bos taurus (cattle)	C3	NP_001035559.2
Mus musculus (house mouse)	C3	NP_033908.2
Rattus norvegicus (Norway rat)	C3	NP_058690.2
Xenopus tropicalis (tropical clawed frog)	LOC100492966	XP_002940099.2
Homo sapiens (human)	C3	NP_000055.2
Gallus gallus (chicken)	C3	NP_990736.1
Danio rerio (zebrafish)	LOC100331428	XP_002660623.3
Xenopus tropicalis (tropical clawed frog)	LOC100491968	XP_004919148.1
Pan troglodytes (chimpanzee)	C3	XP_512318.4
Macaca mulatta (Rhesus monkey)	C3	XP_001091921.2
Danio rerio (zebrafish)	zgc:103710	NP_001008582.3
Danio rerio (zebrafish)	c3b	NP_571318.1
Xenopus tropicalis (tropical clawed frog)	LOC100492134	XP_002940094.2
Xenopus tropicalis (tropical clawed frog)	LOC100497762	XP_004919229.1

Gene Ontology
Bibliography

Related articles in PubMed

The gut mucosal barrier of zebrafish (Danio rerio) responds to the time-restricted delivery of Lobosphaera incisa-enriched diets.
Lazado CC, Nayak S, Khozin-Goldberg I, Zilberg D
Fish & shellfish immunology89368-377(2019 Jun)

Comparative study of stress and immune-related transcript outcomes triggered by Vibrio anguillarum bacterin and air exposure stress in liver and spleen of gilthead seabream (Sparus aurata), zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss).
Khansari AR, Balasch JC, Vallejos-Vidal E, Teles M, Fierro-Castro C, Tort L, Reyes-López FE
Fish & shellfish immunology86436-448(2019 Mar)

Neuroinflammatory signals drive spinal curve formation in zebrafish models of idiopathic scoliosis.
Van Gennip JLM, Boswell CW, Ciruna B
Science advances4(12)eaav1781(2018 12)

Nitrite Enhances MC-LR-Induced Changes on Splenic Oxidation Resistance and Innate Immunity in Male Zebrafish.
Lin W, Guo H, Wang L, Zhang D, Wu X, Li L, Li D, Tang R
Toxins10(12)(2018 12)

Dietary Supplementation With ω6 LC-PUFA-Rich Algae Modulates Zebrafish Immune Function and Improves Resistance to Streptococcal Infection.
Nayak S, Khozin-Goldberg I, Cohen G, Zilberg D
Frontiers in immunology91960(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following c3a.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the c3a.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa147196	XM_021478306.1 Latest version!	Danio rerio complement component c3a, duplicate 2 (c3a.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa32081	NM_131243.1 Latest version!	Danio rerio complement component c3a, duplicate 2 (c3a.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

c3a.2 ( XM_021478306.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333981.1 Danio rerio complement component c3a, duplicate 2 (c3a.2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147196
Clone ID Related Accession (Same CDS sequence)	XM_021478306.1
Accession Version	XM_021478306.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	complement component c3a, duplicate 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGA AGCTGCTGTT TCTCTCTGCT GTTCTTCTCT CCTCAGCGCT TCTCACACTG 
TGTGACCCGC TGTATGTTCT GATGGCTCCT AATCTGCTGA GAGTGGGTTC ATCAGAGAAC 
GTGTTTGTGG AGGCGCAGGA TTATTCTGGA GAACCCCTAA GAGTGAAGAT CTCTGTGAAG 
AACTTCCCAG CCAAGAATGT GGAGATGATG CAAAAAACAG TGACTCTGAC TGGAGAAAAA 
TATCAGATCC TCACAGACAT AGAGATTCCG GATGATAAAA ACTTCTTCTC TGATGATGAG 
AAGAAGCAGT ATGTGTATCT GCAAGCTCAC TTTCCCTCCG TCACTCTGGA GAAAGTGGTC 
ATGGTGTCTT TTCAGTCTGG ATATTTGTTT GTACAGACGG ACAAGCCCAT CTACACGCCT 
GGCAGCAATG TCCAGTACAG GATTTTTTCT ATGACACCCA ACTTGAAACC ACTCAGTCAG 
CCTGGAATCA CAGTGGATAT CATGAATCCA CAAGGCATCA CGGTAGCATC AGATGCAATA 
AAGGCAGAGA AAGGTGTGAA GTCTTCAATC TACAACATCC CTGATGTTAC TAGTTTAGGG 
ATGTGGAAAG TGGTCACGCG GTACACAAAC ACCCCTCTGA AGACATTTAC TGCAGAGTTT 
GAGGTCAAAG AATACGTGCT GCCGACTTTT GAAGTGAAAT TAACACCCAG TAGTTTTTTC 
TACTACGTGG GAGACAACAA ACAAGAGAAC GACGAGGAAG ACAGTCTGAC AGTTGACATC 
GAAGCCAAGT ATCTGTTTGG AAAGAAGATG GATGGAAATG CATTTGTAGT GTTTGGTGTG 
ATGGATGGTG CGGCGAAAAC AAGTATTGTG AACTCTCTGC AAAAAGTTCC GGTTGTGAAT 
GGAGTAGGAA CTGCAGAACT GACCAGGGAG ATGATCACTA AGACATTTAA AAACATAAAA 
GATCTGGTTG GGAAGTCAAT CTATGTTTCA GTCAACCTTT TAACTGAAAG TGGCAGTGAA 
ATGGTGGAAG CAGAAAAGAG AGGCATTCAG ATTGTGACGT CTCCGTACAC CATCCATTAC 
AAAAGAACAT CACAGTTCTT CAAACCTGGA ATGCCTCTGG GTGTCTCGGT TTACGTAACA 
AATCCTGATG AGACGCCTGC AAAAGATGTG GAAGTGGAGG TGCTGGTTGA TGGAAAGGGA 
GGAGTCAGTG GTAAAACCAG AGACAATGGC ATTGTGAAAG TGAAAGTCAA CACTCCGGGA 
GGAGCCTCCA CATTGAAGAT CACTGCGAAG ACCAAAAATA CAGAATACAC CAGTGAACAG 
CAGGCAGTGA AAACAATGAC AGCTCAGGCC TACAAAACCA AACACGACTC GAAAAACTAC 
CTGCATATCA ACATCGAGTC TGCAGAGTTT GAGATTGGTG ATCAAATGAC GGTTAATCTG 
ATCACTGGAC AAAGTTCAGG AGACAGAGAT CAAGATTACA CATACATGAT CTTGAGTAAA 
GGTCAGATTG TTCTAGCAGA AAGATTTAAG AGGCAAGGAC AAACTCTAGT CACTCTGTCT 
TTAACTATCA CTAAAGAAAT GGTGCCGTCC TTCCGCTTTG TGGCTTATTA CCATGTGGGC 
AAAGATGAGG TGGTGTCTGA TTCAGTCTGG GTCGATGTGA AGGACACATG CATGGGAAAG 
CTCAACGTCG AGGTGAAGGA AAAGAAGAAT ACCTATGAAA CAGGAAATGA TGTCAATCTT 
CACATAACTG GAGATCCTGG AGCCAGAGTT GGTCTGGTGG TGGTTGACAA GGCAGTGCAG 
GTCCTTAACA AGAACAGACT CACTCAGACA AAGGTCTGGG ATGTCATTGA GAAGCATGAT 
ACTGGTTGTA CGGGTGGAGG TGGAAAAGAC AGTATGGGGG TTTTTAGTGA TGCTGGTTTG 
ATTTTTGAGT CCAACACCGC AGGAGGAACT GACACCAGAA CAGTGCCTGA GTGTCCTGCC 
CCTCTGAAGA GAAAACGTAG ATCTGAGAGT CTTCAGACAA TCACTACAAC TCTCGCTGGC 
CAATACACTG AAAAAATCCG GCCATGCTGC TATGATGGGA TGAGGAATAA CAGAATTGGC 
TACACATGTG AACGGCGAGC ATCATACGTC ATAGATGGAG AAGAGTGTGT CAAGGCCTTC 
TTGCACTGCT GCAAAGAGGT GAAAAACCAC AAAGAGAAAG AGACTGAAGA GGAAGAGTTG 
ATTCTGGCTC GCAGTGATGA AGACGAGGAA GGGGATTATA AAGACTATGA TGACATTACA 
TCACGCACGC AGTTTCCTGA GAGCTGGCTT TGGGAGGAGT TTGATCTCTG TGACAAGTGT 
TCAAAACCAA GCAAGGACAA AGTGATTTAC CTAAAAGACT CTATTACTAC CTGGCAGATC 
TTGGCTATCA GCCTCTCACC AACACACGGC ATCTGTGTGG CAGAACCTGA GGAGATCGTC 
GTTTTTAAGA GTTTTTTCAT TGACCTCAAA GTGCCTTACT CAGCTGTACG AGGTGAACAA 
GTGGAAATCA AGGCCATTAT TCACAACTAC ACCCCCAAAC ACCTGAAGAA GGTTCGTGTG 
GAGTTTTTGG AGACAGCAGG TGTTTGCAGT GCTGCCAGTA AGAAAGGGAA ATACAGAACA 
ACAGTAAATG TTGACAAGGA CTCCAGCGTA GCAGTTTCCT TTGTGATCGT TCCAATGACA 
CTGGAAAGTC AGCATATTGA GGTGAAAGCT TCAATATCAG ATTATGAAGA TGGAGTTAGA 
AAGACCCTGA AGGTGGTGTC TGAAGGTGTG CTGACTAAAT TTCGAACTGA AAAACTGGAG 
CTTAACCCAG GCAAAAAAAG GGGAAAAACC CTTAGTTCTT CGATCTGGTA TACCAGCAGA 
TCAGGTTCCA GGCACACCAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333981.1
CDS	32..2935
Translation	MEVKLLFLSAVLLSSALLTLCDPLYVLMAPNLLRVGSSENVFVEAQDYSGEPLRVKISVKNFPAKNVEMMQKTVTLTGEKYQILTDIEIPDDKNFFSDDEKKQYVYLQAHFPSVTLEKVVMVSFQSGYLFVQTDKPIYTPGSNVQYRIFSMTPNLKPLSQPGITVDIMNPQGITVASDAIKAEKGVKSSIYNIPDVTSLGMWKVVTRYTNTPLKTFTAEFEVKEYVLPTFEVKLTPSSFFYYVGDNKQENDEEDSLTVDIEAKYLFGKKMDGNAFVVFGVMDGAAKTSIVNSLQKVPVVNGVGTAELTREMITKTFKNIKDLVGKSIYVSVNLLTESGSEMVEAEKRGIQIVTSPYTIHYKRTSQFFKPGMPLGVSVYVTNPDETPAKDVEVEVLVDGKGGVSGKTRDNGIVKVKVNTPGGASTLKITAKTKNTEYTSEQQAVKTMTAQAYKTKHDSKNYLHINIESAEFEIGDQMTVNLITGQSSGDRDQDYTYMILSKGQIVLAERFKRQGQTLVTLSLTITKEMVPSFRFVAYYHVGKDEVVSDSVWVDVKDTCMGKLNVEVKEKKNTYETGNDVNLHITGDPGARVGLVVVDKAVQVLNKNRLTQTKVWDVIEKHDTGCTGGGGKDSMGVFSDAGLIFESNTAGGTDTRTVPECPAPLKRKRRSESLQTITTTLAGQYTEKIRPCCYDGMRNNRIGYTCERRASYVIDGEECVKAFLHCCKEVKNHKEKETEEEELILARSDEDEEGDYKDYDDITSRTQFPESWLWEEFDLCDKCSKPSKDKVIYLKDSITTWQILAISLSPTHGICVAEPEEIVVFKSFFIDLKVPYSAVRGEQVEIKAIIHNYTPKHLKKVRVEFLETAGVCSAASKKGKYRTTVNVDKDSSVAVSFVIVPMTLESQHIEVKASISDYEDGVRKTLKVVSEGVLTKFRTEKLELNPGKKRGKTLSSSIWYTSRSGSRHTS

Target ORF information:

RefSeq Version	XM_021478306.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement component c3a, duplicate 2 (c3a.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478306.1

ORF Insert Sequence:

ATGGAGGTGA AGCTGCTGTT TCTCTCTGCT GTTCTTCTCT CCTCAGCGCT TCTCACACTG 
TGTGACCCGC TGTATGTTCT GATGGCTCCT AATCTGCTGA GAGTGGGTTC ATCAGAGAAC 
GTGTTTGTGG AGGCGCAGGA TTATTCTGGA GAACCCCTAA GAGTGAAGAT CTCTGTGAAG 
AACTTCCCAG CCAAGAATGT GGAGATGATG CAAAAAACAG TGACTCTGAC TGGAGAAAAA 
TATCAGATCC TCACAGACAT AGAGATTCCG GATGATAAAA ACTTCTTCTC TGATGATGAG 
AAGAAGCAGT ATGTGTATCT GCAAGCTCAC TTTCCCTCCG TCACTCTGGA GAAAGTGGTC 
ATGGTGTCTT TTCAGTCTGG ATATTTGTTT GTACAGACGG ACAAGCCCAT CTACACGCCT 
GGCAGCAATG TCCAGTACAG GATTTTTTCT ATGACACCCA ACTTGAAACC ACTCAGTCAG 
CCTGGAATCA CAGTGGATAT CATGAATCCA CAAGGCATCA CGGTAGCATC AGATGCAATA 
AAGGCAGAGA AAGGTGTGAA GTCTTCAATC TACAACATCC CTGATGTTAC TAGTTTAGGG 
ATGTGGAAAG TGGTCACGCG GTACACAAAC ACCCCTCTGA AGACATTTAC TGCAGAGTTT 
GAGGTCAAAG AATACGTGCT GCCGACTTTT GAAGTGAAAT TAACACCCAG TAGTTTTTTC 
TACTACGTGG GAGACAACAA ACAAGAGAAC GACGAGGAAG ACAGTCTGAC AGTTGACATC 
GAAGCCAAGT ATCTGTTTGG AAAGAAGATG GATGGAAATG CATTTGTAGT GTTTGGTGTG 
ATGGATGGTG CGGCGAAAAC AAGTATTGTG AACTCTCTGC AAAAAGTTCC GGTTGTGAAT 
GGAGTAGGAA CTGCAGAACT GACCAGGGAG ATGATCACTA AGACATTTAA AAACATAAAA 
GATCTGGTTG GGAAGTCAAT CTATGTTTCA GTCAACCTTT TAACTGAAAG TGGCAGTGAA 
ATGGTGGAAG CAGAAAAGAG AGGCATTCAG ATTGTGACGT CTCCGTACAC CATCCATTAC 
AAAAGAACAT CACAGTTCTT CAAACCTGGA ATGCCTCTGG GTGTCTCGGT TTACGTAACA 
AATCCTGATG AGACGCCTGC AAAAGATGTG GAAGTGGAGG TGCTGGTTGA TGGAAAGGGA 
GGAGTCAGTG GTAAAACCAG AGACAATGGC ATTGTGAAAG TGAAAGTCAA CACTCCGGGA 
GGAGCCTCCA CATTGAAGAT CACTGCGAAG ACCAAAAATA CAGAATACAC CAGTGAACAG 
CAGGCAGTGA AAACAATGAC AGCTCAGGCC TACAAAACCA AACACGACTC GAAAAACTAC 
CTGCATATCA ACATCGAGTC TGCAGAGTTT GAGATTGGTG ATCAAATGAC GGTTAATCTG 
ATCACTGGAC AAAGTTCAGG AGACAGAGAT CAAGATTACA CATACATGAT CTTGAGTAAA 
GGTCAGATTG TTCTAGCAGA AAGATTTAAG AGGCAAGGAC AAACTCTAGT CACTCTGTCT 
TTAACTATCA CTAAAGAAAT GGTGCCGTCC TTCCGCTTTG TGGCTTATTA CCATGTGGGC 
AAAGATGAGG TGGTGTCTGA TTCAGTCTGG GTCGATGTGA AGGACACATG CATGGGAAAG 
CTCAACGTCG AGGTGAAGGA AAAGAAGAAT ACCTATGAAA CAGGAAATGA TGTCAATCTT 
CACATAACTG GAGATCCTGG AGCCAGAGTT GGTCTGGTGG TGGTTGACAA GGCAGTGCAG 
GTCCTTAACA AGAACAGACT CACTCAGACA AAGGTCTGGG ATGTCATTGA GAAGCATGAT 
ACTGGTTGTA CGGGTGGAGG TGGAAAAGAC AGTATGGGGG TTTTTAGTGA TGCTGGTTTG 
ATTTTTGAGT CCAACACCGC AGGAGGAACT GACACCAGAA CAGTGCCTGA GTGTCCTGCC 
CCTCTGAAGA GAAAACGTAG ATCTGAGAGT CTTCAGACAA TCACTACAAC TCTCGCTGGC 
CAATACACTG AAAAAATCCG GCCATGCTGC TATGATGGGA TGAGGAATAA CAGAATTGGC 
TACACATGTG AACGGCGAGC ATCATACGTC ATAGATGGAG AAGAGTGTGT CAAGGCCTTC 
TTGCACTGCT GCAAAGAGGT GAAAAACCAC AAAGAGAAAG AGACTGAAGA GGAAGAGTTG 
ATTCTGGCTC GCAGTGATGA AGACGAGGAA GGGGATTATA AAGACTATGA TGACATTACA 
TCACGCACGC AGTTTCCTGA GAGCTGGCTT TGGGAGGAGT TTGATCTCTG TGACAAGTGT 
TCAAAACCAA GCAAGGACAA AGTGATTTAC CTAAAAGACT CTATTACTAC CTGGCAGATC 
TTGGCTATCA GCCTCTCACC AACACACGGC ATCTGTGTGG CAGAACCTGA GGAGATCGTC 
GTTTTTAAGA GTTTTTTCAT TGACCTCAAA GTGCCTTACT CAGCTGTACG AGGTGAACAA 
GTGGAAATCA AGGCCATTAT TCACAACTAC ACCCCCAAAC ACCTGAAGAA GGTTCGTGTG 
GAGTTTTTGG AGACAGCAGG TGTTTGCAGT GCTGCCAGTA AGAAAGGGAA ATACAGAACA 
ACAGTAAATG TTGACAAGGA CTCCAGCGTA GCAGTTTCCT TTGTGATCGT TCCAATGACA 
CTGGAAAGTC AGCATATTGA GGTGAAAGCT TCAATATCAG ATTATGAAGA TGGAGTTAGA 
AAGACCCTGA AGGTGGTGTC TGAAGGTGTG CTGACTAAAT TTCGAACTGA AAAACTGGAG 
CTTAACCCAG GCAAAAAAAG GGGAAAAACC CTTAGTTCTT CGATCTGGTA TACCAGCAGA 
TCAGGTTCCA GGCACACCAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

c3a.2 ( NM_131243.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571318.1 Danio rerio complement component c3a, duplicate 2 (c3a.2), mRNA.

Price & Availability↑

CloneID	ODa32081
Clone ID Related Accession (Same CDS sequence)	NM_131243.1
Accession Version	NM_131243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Danio rerio(zebrafish)
Product	complement component c3a, duplicate 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB235997.1. On Nov 23, 2005 this sequence version replaced XM_688299.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB235997.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505386, SAMEA4476730 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGTGA AGCTGCTGTT TCTCTCTGCT GTTCTTCTCT CCTCAGCGCT CCTCACACTG 
TGTGACCCGC TGTATGTTCT GATGGCTCCT AATCTGCTGA GAGTGGGTTC ATCAGAGAAC 
GTGTTTGTGG AGGCGCAGGA TTACTCTGGA GAACCCCTAA GAGTGAAGAT CTCTGTGAAG 
AACTTCCCAG CCAAGAATGT GGAGATGATG CAAAAAACAG TGACTCTGAC TGGAGAAAAA 
TATCAGATCC TCACAGACAT AGAGATTCCG GATGATAAAA ACTTTTTCTC TGATGATGAG 
AAGAAGCAGT ATGTGTATCT GCAAGCTCAC TTTCCCTCCG TCACTCTGGA GAAAGTGGTC 
ATGGTGTCTT TTCAGTCTGG ATATTTGTTT GTACAGACGG ACAAGCCCAT CTACACGCCT 
GGCAGCAATG TCCAGTACAG GATTTTCTCT ATGACACCCA ACTTGAAACC ACTCAGTCAG 
CCTGGAATCA CAGTGGATAT CATGAATCCA CAAGGCATCA CGGTAGCATC AGACGCAATA 
AAGGCAGAGA AAGGTGTGAA GTCTTCAATC TACAACATCC CTGATGTTAC TAGTTTGGGG 
ATGTGGAAAG TGGTCACGCG GTACACAAAC ACCCCTCTGA AGACATTTAC TGCAGAGTTT 
GAGGTCAAAG AATACGTGCT GCCGACTTTT GAAGTGAAAT TAACACCCAG TAGTTTTTTC 
TACTACGTGG GAGACAACAA ACAAGAGAAC GATGAGGAAG ACAGTCTGAC AGTTGACATC 
GAAGCCAAGT ATCTGTTTGG AAAGAAGATG GATGGAAATG CATTTGTAGT GTTTGGTGTG 
ATGGATGGTG CGGCGAAAAC AAGTATTGTG AACTCTCTGC AAAAAGTTCC GGTTGTGAAT 
GGAGTAGGAA CTGCAGAATT GACCAGGGAG ATGATCACTA AGACATTTAA AAACATAAAA 
GATCTGGTTG GGAAATCAAT CTACGTTTCA GTCAACCTTT TAACTGAAAG TGGCAGTGAA 
ATGGTGGAAG CAGAAAAGAG AGGCATTCAG ATTGTGACGT CTCCGTACAC CATCCATTAC 
AAAAGAACAT CACAGTTCTT CAAACCTGGA ATGCCCCTGG GTGTCTCGGT TTACGTAACA 
AATCCTGATG AGACGCCTGC AAAAGATGTG GAAGTGGAGG TGCTGGTTGA TGGAAAGGGA 
GGAGTCAGTG GTAAAACCAG AGACAATGGC ATTGCGAAAG TGAAAGTCAA CACTCCGGGA 
GGAGCCTCCA CATTGAAGAT CACTGCGAAG ACCAAAAATA CAGAATACAC CAGTGAACAG 
CAGGCAGTGA AAACAATGAC AGCTCAGGCC TACAAAACCA AACACGACTC GAAAAACTAC 
CTGCATATCA ACATCGAGTC TGCAGAGTTT GAGATTGGTG ATCAAATGAC GGTTAATCTG 
ATCACTGGAC AAAGTTCAGG AGACAGAGAT CAAGATTACA CATACATGAT CTTGAGTAAA 
GGTCAGATTG TTCTAGCAGA AAGATTTAAG AGGCAAGGAC AAACTCTAGT CACTCTGTCT 
TTAACTATCA CTAAAGAAAT GGTGCCGTCC TTCCGCTTTG TGGCTTATTA CCATGTGGGC 
AAAGATGAGG TGGTGTCTGA TTCAGTCTGG GTCGATGTGA AGGACACATG CATGGGAAAG 
CTCAACGTCG AGGTGAAGGA AAAGAAGAAT ACCTATGAAA CAGGAAATGA TGTCAATCTT 
CACATAACTG GAGATCCTGG AGCCAGAGTT GGTCTGGTGG TGGTTGACAA GGCAGTGCAG 
GTCCTTAACA AGAACAGACT CACTCAGTCG AAGGTCTGGG ATGTCATTGA GAAGCATGAT 
ACTGGTTGTA CGGGTGGAGG TGGAAAAGAC AGTATGGGGG TTTTTAGTGA TGCTGGTTTG 
ATTTTTGAGT CCAACACCGC AGGAGGAACT GACACCAGAA CAGTGCCTGA GTGTCCTGCC 
CCTCTGAAGA GAAAACGGAG ATCTGAGAGT CTTCAGACAA TCACTACAAC TCTCGCTGGC 
CAATACACTG AAAAAATCCG GCCATGCTGC TATGACGGGA TGAGGAATAA CAGAATTGGC 
TACACATGTG AACGGCGAGC ATCATACGTC ATAGATGGAG AAGAGTGTGT CAAGGCCTTC 
TTGCACTGCT GCAAAGAGGT GAAAAACCAC AAAGAGAAAG AGACTGAAGA GGAAGAGCTG 
ATTCTGGCTC GTAGTGATGA AGACGAGGAA GGGGATTATA AAGACTATGA TGACATTACA 
TCACGCACGC AGTTTCCTGA GAGCTGGCTT TGGGAGGAGT TTGATCTCTG TGACAAGTGT 
TCAAAACCAA GCAAGGACAA AGTGATTTAC CTAAAAGACT CTATTACTAC CTGGCAGATC 
TTGGCTATCA GCCTCTCACC AACACACGGC ATCTGTGTGG CAGAACCTGA GGAGATCGTC 
GTTTTTAAGA GTTTTTTCAT TGACCTCAAA GTGCCTTACT CAGCTGTACG AGGTGAACAA 
GTGGAAATCA AGGCCATTAT TCACAACTAC ACCCCCAAAC ACCTGAAGAA GGTTCGTGTG 
GAGTTTTTGG AGACAGCAGG GGTTTGCAGT GCTGCCAGTA AGAAAGGGAA ATACAGAACA 
ACAGTAAATG TTGACAAGGA CTCCAGCGTA GCAGTTTCCT TTGTGATCGT TCCAATGACA 
CTGGAAAGTC AGCATATTGA GGTGAAAGCT TCAATATCAG ATTATGAAGA TGGAGTTAGA 
AAGACCCTGA AGGTGGTGTC TGAAGGTGTG CTGACTAAAT TTCGAACTGA AAAACTGGAG 
CTTAACCCAG GCAAAAAAGG GGAAAAACCC TTAGTTCTTC GATCTGGTAT ACCAGCAGAT 
CAGGTTCCAG GCACACCAGT TAACTCGTAC ATCACAGTTA CTGGTGAAGA AATCAGTCAG 
TCGATTGAGC AGGCCATCAA TGGAAGCTTC ATGGGAGAAC TTATCATCCA GCCTCATGGA 
TGTGGAGAGC AGAATATGGT CACCATGACT TTACCTTTCA TTGCCACTCA TTATCTGGAC 
AACACCAATC AGTGGGAATA TGTTGGCATT GACCGCCGTA AAGTTGCCAT TAACCATATC 
AAAACAGGTT ATGAAGGGCA GCTAAAGTTT CGAAAACAAG ATGGATCTTA CGCTCTCTGG 
ATTGAAACAG ACAGTAGCAC ATGGCTGACA GCATATGTGG CAAAAGTCTT CGCCATGGCA 
AGTAAGCTTA TTGCTATAGA GGAAAATGTG ATCTGCAGCG CCCTCAAGTG GCTAGTTATG 
CACAAACAAC TACCAGATGG CTCTTTTAAA GAGGATAAAC CTGTATATCA CCGGGAAATT 
ATGGGTGGCA TAGAAGGCCA AAACACTGAT GTCTCTCTGA CAGCCTTTGT CTTGATTGCC 
ATGCAAGAAG GCAAAGAGAT CTGTGGGGGA GCAGTTGGAA GTCTGCATGA AAGTATAAGG 
AAGTCAGTTG CTTTCTTGAA AAATCAGCTC ACAAAACTGA CCAATCCTTA TGCAGCTGCG 
TTGACGTCCT ACGCCCTGGC CAATGCCGAC AGTCTTGATA AAGAAATCTT GATGAAGCAT 
TCCACCAAAG GAAACGATGG AAGGTTCTGG AATGTCCCTG GATGGCATTA TCACTCAATA 
GAGGCGACGG CCTATGCTGT GCTGGCACTC GTGAAGGCCA AAGACTATGA TACAGCAGGA 
GAGGCAGTGC ATTGGCTGGC CAAACAAAAA TCTAGTCATG GTGGTTATGA CACTACACAG 
GTGACCATCA TGGTTTTCCA GGCTGTGGCA GAGTACCGTG CTCAGGTGCA AGACCTAAAA 
AACTTTGACC TGAATGTGGA GCTGGTTGTG CCAGGAAGAC GAAAAACTGA CAGATGGACC 
ATTCAAAGAA GTAATGTGCA TCTTACGCGG TCAGGCAGTG TGGATATGAA TAAGGACTTC 
ACTGTGATTG CTAAAGGAAC CGGATCAGCC ATTCTCTCAG TTCTGACGCT GTACTACGCC 
AGACCGTTTG AGAAGAAGAC TGACTGTACT TTCTTTGATC TGAGTGTCAA ATTGGATAAA 
ATCCCTGAAA CAAAACCAGG AACCAATGGA ACGTACAACT TCATCCTGGA TTTCTTCTAC 
AAAAACAAAA CAAATGACGC CACCATGACG ATTCTGGACA TTGGCTTGCC AACAGGATTT 
GATATCGATG AAGCTGATCT TAAACAGTTG TCCACAGGGA AGGAGAGATA CATTCAGAAA 
TATGAAAAGA ATAAAGTGCT CTCAGAGCGA GGATCCCTCA TCATCTACTT AAACAAGGTT 
TCTCATAAGG AATCAGAAAC TCTTGTCTTC AGAATGCACA AGACTTTTAA CGTGGGTTTG 
CTTCAACCTG CTTCCGTCAC CATATATGAG TATTACTCAC CCGATGCCCG CTGTAAAAAG 
TACTACCACC CTGAAAGGGA AGATTCCGCC CTATACAGAC TGTGTAAAGG AGACCTGTGT 
GTGTGTGCTG AAGAAAACTG CAGCTACCAG AAGAAAAACC AAGTCAATGA GTCTGATCGC 
TTGATTAAAG CCTGTGAACC CCATCTGGAT TACATTTATA AAGCGAAATT AGTGAAGATG 
GACCTGAAAC AAGACTCCGA TATCTATCAC ATGAAGGTGG AGAAGACGGT GAAAGAAGGA 
ACTGATATGG ATGTTGGAGG GAAAGTGAAA CCATTTTTGG CTCGTCCCAG TTGCCGAGTG 
AGTTTAGGAC TGGTTGAAGG AAAGTCGTAT CTCATCATGG GAAACTCTGT TGATCTGCCG 
ACCCTTGGGG GAAGTCTGCA GTACATCATA GGAGAGCAGA CCTGGATTGA ATACTGGCCT 
ACAAGTGACG AAAGTAAATC TGCTGAACAC AGAGATCGCT ACATCGGCAT GACTGCAGTC 
GAGAATATGT TTACCACAGG AGGATGTTTG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571318.1
CDS	22..4977
Translation	MEVKLLFLSAVLLSSALLTLCDPLYVLMAPNLLRVGSSENVFVEAQDYSGEPLRVKISVKNFPAKNVEMMQKTVTLTGEKYQILTDIEIPDDKNFFSDDEKKQYVYLQAHFPSVTLEKVVMVSFQSGYLFVQTDKPIYTPGSNVQYRIFSMTPNLKPLSQPGITVDIMNPQGITVASDAIKAEKGVKSSIYNIPDVTSLGMWKVVTRYTNTPLKTFTAEFEVKEYVLPTFEVKLTPSSFFYYVGDNKQENDEEDSLTVDIEAKYLFGKKMDGNAFVVFGVMDGAAKTSIVNSLQKVPVVNGVGTAELTREMITKTFKNIKDLVGKSIYVSVNLLTESGSEMVEAEKRGIQIVTSPYTIHYKRTSQFFKPGMPLGVSVYVTNPDETPAKDVEVEVLVDGKGGVSGKTRDNGIAKVKVNTPGGASTLKITAKTKNTEYTSEQQAVKTMTAQAYKTKHDSKNYLHINIESAEFEIGDQMTVNLITGQSSGDRDQDYTYMILSKGQIVLAERFKRQGQTLVTLSLTITKEMVPSFRFVAYYHVGKDEVVSDSVWVDVKDTCMGKLNVEVKEKKNTYETGNDVNLHITGDPGARVGLVVVDKAVQVLNKNRLTQSKVWDVIEKHDTGCTGGGGKDSMGVFSDAGLIFESNTAGGTDTRTVPECPAPLKRKRRSESLQTITTTLAGQYTEKIRPCCYDGMRNNRIGYTCERRASYVIDGEECVKAFLHCCKEVKNHKEKETEEEELILARSDEDEEGDYKDYDDITSRTQFPESWLWEEFDLCDKCSKPSKDKVIYLKDSITTWQILAISLSPTHGICVAEPEEIVVFKSFFIDLKVPYSAVRGEQVEIKAIIHNYTPKHLKKVRVEFLETAGVCSAASKKGKYRTTVNVDKDSSVAVSFVIVPMTLESQHIEVKASISDYEDGVRKTLKVVSEGVLTKFRTEKLELNPGKKGEKPLVLRSGIPADQVPGTPVNSYITVTGEEISQSIEQAINGSFMGELIIQPHGCGEQNMVTMTLPFIATHYLDNTNQWEYVGIDRRKVAINHIKTGYEGQLKFRKQDGSYALWIETDSSTWLTAYVAKVFAMASKLIAIEENVICSALKWLVMHKQLPDGSFKEDKPVYHREIMGGIEGQNTDVSLTAFVLIAMQEGKEICGGAVGSLHESIRKSVAFLKNQLTKLTNPYAAALTSYALANADSLDKEILMKHSTKGNDGRFWNVPGWHYHSIEATAYAVLALVKAKDYDTAGEAVHWLAKQKSSHGGYDTTQVTIMVFQAVAEYRAQVQDLKNFDLNVELVVPGRRKTDRWTIQRSNVHLTRSGSVDMNKDFTVIAKGTGSAILSVLTLYYARPFEKKTDCTFFDLSVKLDKIPETKPGTNGTYNFILDFFYKNKTNDATMTILDIGLPTGFDIDEADLKQLSTGKERYIQKYEKNKVLSERGSLIIYLNKVSHKESETLVFRMHKTFNVGLLQPASVTIYEYYSPDARCKKYYHPEREDSALYRLCKGDLCVCAEENCSYQKKNQVNESDRLIKACEPHLDYIYKAKLVKMDLKQDSDIYHMKVEKTVKEGTDMDVGGKVKPFLARPSCRVSLGLVEGKSYLIMGNSVDLPTLGGSLQYIIGEQTWIEYWPTSDESKSAEHRDRYIGMTAVENMFTTGGCLT

Target ORF information:

RefSeq Version	NM_131243.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement component c3a, duplicate 2 (c3a.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131243.1

ORF Insert Sequence:

ATGGAGGTGA AGCTGCTGTT TCTCTCTGCT GTTCTTCTCT CCTCAGCGCT CCTCACACTG 
TGTGACCCGC TGTATGTTCT GATGGCTCCT AATCTGCTGA GAGTGGGTTC ATCAGAGAAC 
GTGTTTGTGG AGGCGCAGGA TTACTCTGGA GAACCCCTAA GAGTGAAGAT CTCTGTGAAG 
AACTTCCCAG CCAAGAATGT GGAGATGATG CAAAAAACAG TGACTCTGAC TGGAGAAAAA 
TATCAGATCC TCACAGACAT AGAGATTCCG GATGATAAAA ACTTTTTCTC TGATGATGAG 
AAGAAGCAGT ATGTGTATCT GCAAGCTCAC TTTCCCTCCG TCACTCTGGA GAAAGTGGTC 
ATGGTGTCTT TTCAGTCTGG ATATTTGTTT GTACAGACGG ACAAGCCCAT CTACACGCCT 
GGCAGCAATG TCCAGTACAG GATTTTCTCT ATGACACCCA ACTTGAAACC ACTCAGTCAG 
CCTGGAATCA CAGTGGATAT CATGAATCCA CAAGGCATCA CGGTAGCATC AGACGCAATA 
AAGGCAGAGA AAGGTGTGAA GTCTTCAATC TACAACATCC CTGATGTTAC TAGTTTGGGG 
ATGTGGAAAG TGGTCACGCG GTACACAAAC ACCCCTCTGA AGACATTTAC TGCAGAGTTT 
GAGGTCAAAG AATACGTGCT GCCGACTTTT GAAGTGAAAT TAACACCCAG TAGTTTTTTC 
TACTACGTGG GAGACAACAA ACAAGAGAAC GATGAGGAAG ACAGTCTGAC AGTTGACATC 
GAAGCCAAGT ATCTGTTTGG AAAGAAGATG GATGGAAATG CATTTGTAGT GTTTGGTGTG 
ATGGATGGTG CGGCGAAAAC AAGTATTGTG AACTCTCTGC AAAAAGTTCC GGTTGTGAAT 
GGAGTAGGAA CTGCAGAATT GACCAGGGAG ATGATCACTA AGACATTTAA AAACATAAAA 
GATCTGGTTG GGAAATCAAT CTACGTTTCA GTCAACCTTT TAACTGAAAG TGGCAGTGAA 
ATGGTGGAAG CAGAAAAGAG AGGCATTCAG ATTGTGACGT CTCCGTACAC CATCCATTAC 
AAAAGAACAT CACAGTTCTT CAAACCTGGA ATGCCCCTGG GTGTCTCGGT TTACGTAACA 
AATCCTGATG AGACGCCTGC AAAAGATGTG GAAGTGGAGG TGCTGGTTGA TGGAAAGGGA 
GGAGTCAGTG GTAAAACCAG AGACAATGGC ATTGCGAAAG TGAAAGTCAA CACTCCGGGA 
GGAGCCTCCA CATTGAAGAT CACTGCGAAG ACCAAAAATA CAGAATACAC CAGTGAACAG 
CAGGCAGTGA AAACAATGAC AGCTCAGGCC TACAAAACCA AACACGACTC GAAAAACTAC 
CTGCATATCA ACATCGAGTC TGCAGAGTTT GAGATTGGTG ATCAAATGAC GGTTAATCTG 
ATCACTGGAC AAAGTTCAGG AGACAGAGAT CAAGATTACA CATACATGAT CTTGAGTAAA 
GGTCAGATTG TTCTAGCAGA AAGATTTAAG AGGCAAGGAC AAACTCTAGT CACTCTGTCT 
TTAACTATCA CTAAAGAAAT GGTGCCGTCC TTCCGCTTTG TGGCTTATTA CCATGTGGGC 
AAAGATGAGG TGGTGTCTGA TTCAGTCTGG GTCGATGTGA AGGACACATG CATGGGAAAG 
CTCAACGTCG AGGTGAAGGA AAAGAAGAAT ACCTATGAAA CAGGAAATGA TGTCAATCTT 
CACATAACTG GAGATCCTGG AGCCAGAGTT GGTCTGGTGG TGGTTGACAA GGCAGTGCAG 
GTCCTTAACA AGAACAGACT CACTCAGTCG AAGGTCTGGG ATGTCATTGA GAAGCATGAT 
ACTGGTTGTA CGGGTGGAGG TGGAAAAGAC AGTATGGGGG TTTTTAGTGA TGCTGGTTTG 
ATTTTTGAGT CCAACACCGC AGGAGGAACT GACACCAGAA CAGTGCCTGA GTGTCCTGCC 
CCTCTGAAGA GAAAACGGAG ATCTGAGAGT CTTCAGACAA TCACTACAAC TCTCGCTGGC 
CAATACACTG AAAAAATCCG GCCATGCTGC TATGACGGGA TGAGGAATAA CAGAATTGGC 
TACACATGTG AACGGCGAGC ATCATACGTC ATAGATGGAG AAGAGTGTGT CAAGGCCTTC 
TTGCACTGCT GCAAAGAGGT GAAAAACCAC AAAGAGAAAG AGACTGAAGA GGAAGAGCTG 
ATTCTGGCTC GTAGTGATGA AGACGAGGAA GGGGATTATA AAGACTATGA TGACATTACA 
TCACGCACGC AGTTTCCTGA GAGCTGGCTT TGGGAGGAGT TTGATCTCTG TGACAAGTGT 
TCAAAACCAA GCAAGGACAA AGTGATTTAC CTAAAAGACT CTATTACTAC CTGGCAGATC 
TTGGCTATCA GCCTCTCACC AACACACGGC ATCTGTGTGG CAGAACCTGA GGAGATCGTC 
GTTTTTAAGA GTTTTTTCAT TGACCTCAAA GTGCCTTACT CAGCTGTACG AGGTGAACAA 
GTGGAAATCA AGGCCATTAT TCACAACTAC ACCCCCAAAC ACCTGAAGAA GGTTCGTGTG 
GAGTTTTTGG AGACAGCAGG GGTTTGCAGT GCTGCCAGTA AGAAAGGGAA ATACAGAACA 
ACAGTAAATG TTGACAAGGA CTCCAGCGTA GCAGTTTCCT TTGTGATCGT TCCAATGACA 
CTGGAAAGTC AGCATATTGA GGTGAAAGCT TCAATATCAG ATTATGAAGA TGGAGTTAGA 
AAGACCCTGA AGGTGGTGTC TGAAGGTGTG CTGACTAAAT TTCGAACTGA AAAACTGGAG 
CTTAACCCAG GCAAAAAAGG GGAAAAACCC TTAGTTCTTC GATCTGGTAT ACCAGCAGAT 
CAGGTTCCAG GCACACCAGT TAACTCGTAC ATCACAGTTA CTGGTGAAGA AATCAGTCAG 
TCGATTGAGC AGGCCATCAA TGGAAGCTTC ATGGGAGAAC TTATCATCCA GCCTCATGGA 
TGTGGAGAGC AGAATATGGT CACCATGACT TTACCTTTCA TTGCCACTCA TTATCTGGAC 
AACACCAATC AGTGGGAATA TGTTGGCATT GACCGCCGTA AAGTTGCCAT TAACCATATC 
AAAACAGGTT ATGAAGGGCA GCTAAAGTTT CGAAAACAAG ATGGATCTTA CGCTCTCTGG 
ATTGAAACAG ACAGTAGCAC ATGGCTGACA GCATATGTGG CAAAAGTCTT CGCCATGGCA 
AGTAAGCTTA TTGCTATAGA GGAAAATGTG ATCTGCAGCG CCCTCAAGTG GCTAGTTATG 
CACAAACAAC TACCAGATGG CTCTTTTAAA GAGGATAAAC CTGTATATCA CCGGGAAATT 
ATGGGTGGCA TAGAAGGCCA AAACACTGAT GTCTCTCTGA CAGCCTTTGT CTTGATTGCC 
ATGCAAGAAG GCAAAGAGAT CTGTGGGGGA GCAGTTGGAA GTCTGCATGA AAGTATAAGG 
AAGTCAGTTG CTTTCTTGAA AAATCAGCTC ACAAAACTGA CCAATCCTTA TGCAGCTGCG 
TTGACGTCCT ACGCCCTGGC CAATGCCGAC AGTCTTGATA AAGAAATCTT GATGAAGCAT 
TCCACCAAAG GAAACGATGG AAGGTTCTGG AATGTCCCTG GATGGCATTA TCACTCAATA 
GAGGCGACGG CCTATGCTGT GCTGGCACTC GTGAAGGCCA AAGACTATGA TACAGCAGGA 
GAGGCAGTGC ATTGGCTGGC CAAACAAAAA TCTAGTCATG GTGGTTATGA CACTACACAG 
GTGACCATCA TGGTTTTCCA GGCTGTGGCA GAGTACCGTG CTCAGGTGCA AGACCTAAAA 
AACTTTGACC TGAATGTGGA GCTGGTTGTG CCAGGAAGAC GAAAAACTGA CAGATGGACC 
ATTCAAAGAA GTAATGTGCA TCTTACGCGG TCAGGCAGTG TGGATATGAA TAAGGACTTC 
ACTGTGATTG CTAAAGGAAC CGGATCAGCC ATTCTCTCAG TTCTGACGCT GTACTACGCC 
AGACCGTTTG AGAAGAAGAC TGACTGTACT TTCTTTGATC TGAGTGTCAA ATTGGATAAA 
ATCCCTGAAA CAAAACCAGG AACCAATGGA ACGTACAACT TCATCCTGGA TTTCTTCTAC 
AAAAACAAAA CAAATGACGC CACCATGACG ATTCTGGACA TTGGCTTGCC AACAGGATTT 
GATATCGATG AAGCTGATCT TAAACAGTTG TCCACAGGGA AGGAGAGATA CATTCAGAAA 
TATGAAAAGA ATAAAGTGCT CTCAGAGCGA GGATCCCTCA TCATCTACTT AAACAAGGTT 
TCTCATAAGG AATCAGAAAC TCTTGTCTTC AGAATGCACA AGACTTTTAA CGTGGGTTTG 
CTTCAACCTG CTTCCGTCAC CATATATGAG TATTACTCAC CCGATGCCCG CTGTAAAAAG 
TACTACCACC CTGAAAGGGA AGATTCCGCC CTATACAGAC TGTGTAAAGG AGACCTGTGT 
GTGTGTGCTG AAGAAAACTG CAGCTACCAG AAGAAAAACC AAGTCAATGA GTCTGATCGC 
TTGATTAAAG CCTGTGAACC CCATCTGGAT TACATTTATA AAGCGAAATT AGTGAAGATG 
GACCTGAAAC AAGACTCCGA TATCTATCAC ATGAAGGTGG AGAAGACGGT GAAAGAAGGA 
ACTGATATGG ATGTTGGAGG GAAAGTGAAA CCATTTTTGG CTCGTCCCAG TTGCCGAGTG 
AGTTTAGGAC TGGTTGAAGG AAAGTCGTAT CTCATCATGG GAAACTCTGT TGATCTGCCG 
ACCCTTGGGG GAAGTCTGCA GTACATCATA GGAGAGCAGA CCTGGATTGA ATACTGGCCT 
ACAAGTGACG AAAGTAAATC TGCTGAACAC AGAGATCGCT ACATCGGCAT GACTGCAGTC 
GAGAATATGT TTACCACAGG AGGATGTTTG ACATAA