PIK3R4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIK3R4
Entrez Gene ID	564088
Full Name	phosphoinositide-3-kinase, regulatory subunit 4
Synonyms	im:7148788,vps15
General protein information	Preferred Names phosphoinositide-3-kinase, regulatory subunit 4 Names phosphoinositide 3-kinase regulatory subunit 4
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021466975.1	XP_021322650.1	phosphoinositide 3-kinase regulatory subunit 4 isoform X2
XM_005158299.4	XP_005158356.1	phosphoinositide 3-kinase regulatory subunit 4 isoform X3
XM_001922641.5	XP_001922676.1	phosphoinositide 3-kinase regulatory subunit 4 isoform X1
XM_001922641.6	XP_001922676.1	phosphoinositide 3-kinase regulatory subunit 4 isoform X1
XM_005158299.3	XP_005158356.1	phosphoinositide 3-kinase regulatory subunit 4 isoform X2
XM_005158300.3	XP_005158357.1	phosphoinositide 3-kinase regulatory subunit 4 isoform X3

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0796700	NP_001048391.1
Gallus gallus (chicken)	PIK3R4	XP_004939498.1
Danio rerio (zebrafish)	pik3r4	XP_001922676.1
Magnaporthe oryzae (rice blast fungus)	MGG_06100	XP_003711966.1
Eremothecium gossypii	AGOS_ADL316C	NP_983780.2
Neurospora crassa	NCU06626	XP_962368.2
Canis lupus familiaris (dog)	PIK3R4	XP_534260.1
Bos taurus (cattle)	PIK3R4	NP_001093784.1
Schizosaccharomyces pombe (fission yeast)	ppk19	NP_595288.1
Macaca mulatta (Rhesus monkey)	PIK3R4	XP_002802990.1
Mus musculus (house mouse)	Pik3r4	NP_001074778.1
Rattus norvegicus (Norway rat)	Pik3r4	NP_001102247.1
Saccharomyces cerevisiae (baker's yeast)	VPS15	NP_009655.2
Xenopus tropicalis (tropical clawed frog)	pik3r4	NP_001107701.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000602	XP_310476.5
Pan troglodytes (chimpanzee)	PIK3R4	XP_516746.2
Drosophila melanogaster (fruit fly)	ird1	NP_649868.1
Arabidopsis thaliana (thale cress)	AT4G29380	NP_194667.1
Kluyveromyces lactis	KLLA0B11924g	XP_452060.1
Homo sapiens (human)	PIK3R4	NP_055417.1

Gene Ontology

The following PIK3R4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIK3R4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa21702	XM_005158300.3 Latest version!	Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa149425	XM_021466975.1 Latest version!	Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa21700	XM_001922641.6 Latest version!	Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa21701	XM_005158299.4 Latest version!	Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
ODa21700	XM_001922641.5	Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa21701	XM_005158299.3	Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIK3R4 ( XM_005158300.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005158357.1 Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa21702
Clone ID Related Accession (Same CDS sequence)	XM_005158300.3
Accession Version	XM_005158300.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	phosphoinositide 3-kinase regulatory subunit 4 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158300.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAACC AACTGGCTGG AATTGCTCCA TCGCAGATCC TGTCAGTGGA CAGTTACTTC 
TCTGACATCC ATGACTACGA ATATGACAAA AGTCTGGGCA GTACACGATT TTTCAAAGTG 
GCCCGGGCGA AACACAGGGA AGGATTGGTC GTTGTTAAGG TCTTTGCTAT CCAAGACCCT 
TCTCTACCCC TGACCAGCTA CAAACAAGAG GTGGAGGAGC TGAAAATCCG ACTGCACTCC 
TGTCAGAACT GCCTGCCTTT CCAAAAAGCC ACGCTTACTG AAAAAGCAGC CATTCTGTTT 
CGCCAGTATG TTCGGGATAA CTTGTATGAC CGTATTAGTA CGCGGCCTTT CCTCAACAAC 
GTGGAAAAGA AGTGGATCGC CTTCCAGCTC CTCAATGCTG TGGACCAGGC CCATAAATCG 
GGAGTGCGTC ACGGAGACAT TAAAACAGAG AACGTCATGG TGACAAGCTG GAACTGGGTG 
CTTCTAACAG ACTTTGCTAG CTTTAAGCCA ACATACTTGC CTGAAGACAA TCCCGCAGAT 
TTTAATTACT TCTTCGACAC GTCCAGGAGG AGAACATGTT ACATTGCACC TGAGAGATTT 
GTGGATGGCA GCATGTTTGC TACAGAAAGT GACCAGACCA CTCCTCTCGT AGATCTTTCT 
AGTAATAGTC AGAGGACCAG AGGGGAGCTC AAGCAGCCCA TGGACATCTT CTCAGCTGGT 
TGTGTGATTG CGGAATTGTT CACAGAGGGC GTCCCGCTCT TTGACCTCTC ACAACTGCTG 
GCTTATCGTA AAGGGCATTT CCAAACCGAA CAAGTGCTGA TGAAAATTGA AGATCACAGT 
ATTCGAGAAC TGGTGGCTCA AATGGTGCAG AGAGAGCCAG AAAAGCGACT GACAGCTGAA 
GAATACCTTA AGCAGCAGCG AGGAAAAGCT TTCCCAGAAA TCTTCTACAC CTTCCTTCAA 
CCGTACATGG CCCAGTTTGC CAAGGAGACC TTCCAGTCTG CTGACGAAAG AGTCTTGGTG 
ATCCGTAAAA ACCTGGAGAA CATCCTCAAC AACCTGTGTA GCGGCGGGCA GACGGCGAAG 
GCTGAAAAAC AGGAGAAAGC ATCCCAAGAG TTTAGCTCAT CTAAAGAACA AGGCCTGGTG 
GTGCTGGTGT CAGTGATCAC TTCATGTCTA CAGACGCTTC GATTTTGTGA CTCCAAGCTG 
GCGGCGCTGG AGCTGATCCT TCATCTGGCA GGCCGGCTCA ATGTGGAGAT TCTGCTGGAC 
AGGATTACGC CGTATTTGCT GCACTTCTGC AATGACTCTA TGCCGCGTGT CAGAGCTGAG 
GCCGTTCGCA CGCTGACTAA GGTGCTGGCG CTGGTCAAGG AGGTTCCCCG CAATGATGTC 
AACATCTACC CGGAGTACAT TTTGCCTGGC ATTGCCCACT TGGCCCAAGA TGAAGCCACG 
ATTGTCAGAC TTGCTTATGC AGAAAACATA GCCCATCTAG CCGAGACAGC ACTGCGTTTT 
CTGGAGCTGG TGCAGGAGAA TAATTTGAGC TCTGAACACG ATCTGAATGG AGAGGACACA 
GAAGAAACTC TTCATCCCAA TGAAAACTAT GATTCAGAGT TGCAGGCCTT GCATGAGATG 
GTTCAGCAGA AGGTGGTGAC TTTGCTGAGC GATCCTGAAA ACATCGTCAA ACAGACGCTG 
ATGGAGAATG GCATTACGCG CCTCTGCGTG TTCTTCGGCA GGCAGAAAGC CAATGACGTC 
CTTCTATCCC ACATGATCAC CTTCCTGAAT GACAAGAACG ACTGGCATCT CCGCGGAGCT 
TTCTTTGACA GCATTGTAGG TGTAGCAGCA TATGTGGGCT GGCAGAGCTC CTCCATCCTG 
AAGCCTCTTC TCCAGCAAGG CCTGAGTGAC GTTGAAGAGT TTGTCATTTA TAAAGCTCTC 
AATGCTCTGA CCTGCATGTG TCAGCTGGGG CTGTTGCAGA AGCCTCACAT CTATGAGTTT 
GTCAGCGATA TTGCTCCATT CTTGTGCCAC CCCAATCTGT GGATCCGCTA TGGAGCTGTG 
GGTTTCATCA CTGTCGTCGC TCAGCACCTA AACATCGCTG ATGTTTACTG CAAACTTATG 
CCACATCTTA ACCCTTTCAT CACACAACCC ATTATACAGA TTGATAAGGA GATTGTGTTG 
CTCAGTGTGC TGAAAGAGCC TGTAAGTCGC TCCATCTTTG ACTATGCACT GCGTTCCAAA 
GACATCGGAA GCCTTTTCAG ACACCTGTTA CTCCGGCAAA AGAAGCGCAA CGGCTCCATC 
CCAGAATGCC CCATTCCTGA GGATCCTGCA ATTGCCCAGC TTCTTAAAAA ACTTCTCTCA 
CAGGGCATGA CAGAGGCTGA AGAGGATAAG CTCTTGGCTC TGAAAGATTT CATGCTCAAG 
TCTAACAAGG CCAAAGCCAA CATCATAGAC CAGAGCCACC TGAGTGAAGG AGCCCACAGT 
GGAGTCATTG ATCTCGGGAC ACTGGGCATC ACTGGCCGAC AGGTGGACCT GGTCAAGCCC 
AAGCAGGAGA TTGAAGATAA GAGAGAGTTC ATCTCTTCTG CCTCTGGTAT ATTGTTTAGA 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158357.1
CDS	313..2898
Translation	MGNQLAGIAPSQILSVDSYFSDIHDYEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPSLPLTSYKQEVEELKIRLHSCQNCLPFQKATLTEKAAILFRQYVRDNLYDRISTRPFLNNVEKKWIAFQLLNAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGSMFATESDQTTPLVDLSSNSQRTRGELKQPMDIFSAGCVIAELFTEGVPLFDLSQLLAYRKGHFQTEQVLMKIEDHSIRELVAQMVQREPEKRLTAEEYLKQQRGKAFPEIFYTFLQPYMAQFAKETFQSADERVLVIRKNLENILNNLCSGGQTAKAEKQEKASQEFSSSKEQGLVVLVSVITSCLQTLRFCDSKLAALELILHLAGRLNVEILLDRITPYLLHFCNDSMPRVRAEAVRTLTKVLALVKEVPRNDVNIYPEYILPGIAHLAQDEATIVRLAYAENIAHLAETALRFLELVQENNLSSEHDLNGEDTEETLHPNENYDSELQALHEMVQQKVVTLLSDPENIVKQTLMENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDVEEFVIYKALNALTCMCQLGLLQKPHIYEFVSDIAPFLCHPNLWIRYGAVGFITVVAQHLNIADVYCKLMPHLNPFITQPIIQIDKEIVLLSVLKEPVSRSIFDYALRSKDIGSLFRHLLLRQKKRNGSIPECPIPEDPAIAQLLKKLLSQGMTEAEEDKLLALKDFMLKSNKAKANIIDQSHLSEGAHSGVIDLGTLGITGRQVDLVKPKQEIEDKREFISSASGILFRC

Target ORF information:

RefSeq Version	XM_005158300.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158300.3

ORF Insert Sequence:

ATGGGGAACC AACTGGCTGG AATTGCTCCA TCGCAGATCC TGTCAGTGGA CAGTTACTTC 
TCTGACATCC ATGACTACGA ATATGACAAA AGTCTGGGCA GTACACGATT TTTCAAAGTG 
GCCCGGGCGA AACACAGGGA AGGATTGGTC GTTGTTAAGG TCTTTGCTAT CCAAGACCCT 
TCTCTACCCC TGACCAGCTA CAAACAAGAG GTGGAGGAGC TGAAAATCCG ACTGCACTCC 
TGTCAGAACT GCCTGCCTTT CCAAAAAGCC ACGCTTACTG AAAAAGCAGC CATTCTGTTT 
CGCCAGTATG TTCGGGATAA CTTGTATGAC CGTATTAGTA CGCGGCCTTT CCTCAACAAC 
GTGGAAAAGA AGTGGATCGC CTTCCAGCTC CTCAATGCTG TGGACCAGGC CCATAAATCG 
GGAGTGCGTC ACGGAGACAT TAAAACAGAG AACGTCATGG TGACAAGCTG GAACTGGGTG 
CTTCTAACAG ACTTTGCTAG CTTTAAGCCA ACATACTTGC CTGAAGACAA TCCCGCAGAT 
TTTAATTACT TCTTCGACAC GTCCAGGAGG AGAACATGTT ACATTGCACC TGAGAGATTT 
GTGGATGGCA GCATGTTTGC TACAGAAAGT GACCAGACCA CTCCTCTCGT AGATCTTTCT 
AGTAATAGTC AGAGGACCAG AGGGGAGCTC AAGCAGCCCA TGGACATCTT CTCAGCTGGT 
TGTGTGATTG CGGAATTGTT CACAGAGGGC GTCCCGCTCT TTGACCTCTC ACAACTGCTG 
GCTTATCGTA AAGGGCATTT CCAAACCGAA CAAGTGCTGA TGAAAATTGA AGATCACAGT 
ATTCGAGAAC TGGTGGCTCA AATGGTGCAG AGAGAGCCAG AAAAGCGACT GACAGCTGAA 
GAATACCTTA AGCAGCAGCG AGGAAAAGCT TTCCCAGAAA TCTTCTACAC CTTCCTTCAA 
CCGTACATGG CCCAGTTTGC CAAGGAGACC TTCCAGTCTG CTGACGAAAG AGTCTTGGTG 
ATCCGTAAAA ACCTGGAGAA CATCCTCAAC AACCTGTGTA GCGGCGGGCA GACGGCGAAG 
GCTGAAAAAC AGGAGAAAGC ATCCCAAGAG TTTAGCTCAT CTAAAGAACA AGGCCTGGTG 
GTGCTGGTGT CAGTGATCAC TTCATGTCTA CAGACGCTTC GATTTTGTGA CTCCAAGCTG 
GCGGCGCTGG AGCTGATCCT TCATCTGGCA GGCCGGCTCA ATGTGGAGAT TCTGCTGGAC 
AGGATTACGC CGTATTTGCT GCACTTCTGC AATGACTCTA TGCCGCGTGT CAGAGCTGAG 
GCCGTTCGCA CGCTGACTAA GGTGCTGGCG CTGGTCAAGG AGGTTCCCCG CAATGATGTC 
AACATCTACC CGGAGTACAT TTTGCCTGGC ATTGCCCACT TGGCCCAAGA TGAAGCCACG 
ATTGTCAGAC TTGCTTATGC AGAAAACATA GCCCATCTAG CCGAGACAGC ACTGCGTTTT 
CTGGAGCTGG TGCAGGAGAA TAATTTGAGC TCTGAACACG ATCTGAATGG AGAGGACACA 
GAAGAAACTC TTCATCCCAA TGAAAACTAT GATTCAGAGT TGCAGGCCTT GCATGAGATG 
GTTCAGCAGA AGGTGGTGAC TTTGCTGAGC GATCCTGAAA ACATCGTCAA ACAGACGCTG 
ATGGAGAATG GCATTACGCG CCTCTGCGTG TTCTTCGGCA GGCAGAAAGC CAATGACGTC 
CTTCTATCCC ACATGATCAC CTTCCTGAAT GACAAGAACG ACTGGCATCT CCGCGGAGCT 
TTCTTTGACA GCATTGTAGG TGTAGCAGCA TATGTGGGCT GGCAGAGCTC CTCCATCCTG 
AAGCCTCTTC TCCAGCAAGG CCTGAGTGAC GTTGAAGAGT TTGTCATTTA TAAAGCTCTC 
AATGCTCTGA CCTGCATGTG TCAGCTGGGG CTGTTGCAGA AGCCTCACAT CTATGAGTTT 
GTCAGCGATA TTGCTCCATT CTTGTGCCAC CCCAATCTGT GGATCCGCTA TGGAGCTGTG 
GGTTTCATCA CTGTCGTCGC TCAGCACCTA AACATCGCTG ATGTTTACTG CAAACTTATG 
CCACATCTTA ACCCTTTCAT CACACAACCC ATTATACAGA TTGATAAGGA GATTGTGTTG 
CTCAGTGTGC TGAAAGAGCC TGTAAGTCGC TCCATCTTTG ACTATGCACT GCGTTCCAAA 
GACATCGGAA GCCTTTTCAG ACACCTGTTA CTCCGGCAAA AGAAGCGCAA CGGCTCCATC 
CCAGAATGCC CCATTCCTGA GGATCCTGCA ATTGCCCAGC TTCTTAAAAA ACTTCTCTCA 
CAGGGCATGA CAGAGGCTGA AGAGGATAAG CTCTTGGCTC TGAAAGATTT CATGCTCAAG 
TCTAACAAGG CCAAAGCCAA CATCATAGAC CAGAGCCACC TGAGTGAAGG AGCCCACAGT 
GGAGTCATTG ATCTCGGGAC ACTGGGCATC ACTGGCCGAC AGGTGGACCT GGTCAAGCCC 
AAGCAGGAGA TTGAAGATAA GAGAGAGTTC ATCTCTTCTG CCTCTGGTAT ATTGTTTAGA 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3R4 ( XM_021466975.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021322650.1 Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa149425
Clone ID Related Accession (Same CDS sequence)	XM_021466975.1
Accession Version	XM_021466975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	phosphoinositide 3-kinase regulatory subunit 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAACC AACTGGCTGG AATTGCTCCA TCGCAGATCC TGTCAGTGGA CAGTTACTTC 
TCTGACATCC ATGACTACGA ATATGACAAA AGTCTGGGCA GTACACGATT TTTCAAAGTG 
GCCCGGGCGA AACACAGGGA AGGATTGGTC GTTGTTAAGG TCTTTGCTAT CCAAGACCCT 
TCTCTACCCC TGACCAGCTA CAAACAAGAG GTGGAGGAGC TGAAAATCCG ACTGCACTCC 
TGTCAGAACT GCCTGCCTTT CCAAAAAGCC ACGCTTACTG AAAAAGCAGC CATTCTGTTT 
CGCCAGTATG TTCGGGATAA CTTGTATGAC CGTATTAGTA CGCGGCCTTT CCTCAACAAC 
GTGGAAAAGA AGTGGATCGC CTTCCAGCTC CTCAATGCTG TGGACCAGGC CCATAAATCG 
GGAGTGCGTC ACGGAGACAT TAAAACAGAG AACGTCATGG TGACAAGCTG GAACTGGGTG 
CTTCTAACAG ACTTTGCTAG CTTTAAGCCA ACATACTTGC CTGAAGACAA TCCCGCAGAT 
TTTAATTACT TCTTCGACAC GTCCAGGAGG AGAACATGTT ACATTGCACC TGAGAGATTT 
GTGGATGGCA GCATGTTTGC TACAGAAAGT GACCAGACCA CTCCTCTCGT AGATCTTTCT 
AGTAATAGTC AGAGGACCAG AGGGGAGCTC AAGCAGCCCA TGGACATCTT CTCAGCTGGT 
TGTGTGATTG CGGAATTGTT CACAGAGGGC GTCCCGCTCT TTGACCTCTC ACAACTGCTG 
GCTTATCGTA AAGGGCATTT CCAAACCGAA CAAGTGCTGA TGAAAATTGA AGATCACAGT 
ATTCGAGAAC TGGTGGCTCA AATGGTGCAG AGAGAGCCAG AAAAGCGACT GACAGCTGAA 
GAATACCTTA AGCAGCAGCG AGGAAAAGCT TTCCCAGAAA TCTTCTACAC CTTCCTTCAA 
CCGTACATGG CCCAGTTTGC CAAGGAGACC TTCCAGTCTG CTGACGAAAG AGTCTTGGTG 
ATCCGTAAAA ACCTGGAGAA CATCCTCAAC AACCTGTGTA GCGGCGGGCA GACGGCGAAG 
GCTGAAAAAC AGGAGAAAGC ATCCCAAGAG TTTAGCTCAT CTAAAGAACA AGGCCTGGTG 
GTGCTGGTGT CAGTGATCAC TTCATGTCTA CAGACGCTTC GATTTTGTGA CTCCAAGCTG 
GCGGCGCTGG AGCTGATCCT TCATCTGGCA GGCCGGCTCA ATGTGGAGAT TCTGCTGGAC 
AGGATTACGC CGTATTTGCT GCACTTCTGC AATGACTCTA TGCCGCGTGT CAGAGCTGAG 
GCCGTTCGCA CGCTGACTAA GGTGCTGGCG CTGGTCAAGG AGGTTCCCCG CAATGATGTC 
AACATCTACC CGGAGTACAT TTTGCCTGGC ATTGCCCACT TGGCCCAAGA TGAAGCCACG 
ATTGTCAGAC TTGCTTATGC AGAAAACATA GCCCATCTAG CCGAGACAGC ACTGCGTTTT 
CTGGAGCTGG TGCAGGAGAA TAATTTGAGC TCTGAACACG ATCTGAATGG AGAGGACACA 
GAAGAAACTC TTCATCCCAA TGAAAACTAT GATTCAGAGT TGCAGGCCTT GCATGAGATG 
GTTCAGCAGA AGGTGGTGAC TTTGCTGAGC GATCCTGAAA ACATCGTCAA ACAGACGCTG 
ATGGAGAATG GCATTACGCG CCTCTGCGTG TTCTTCGGCA GGCAGAAAGC CAATGACGTC 
CTTCTATCCC ACATGATCAC CTTCCTGAAT GACAAGAACG ACTGGCATCT CCGCGGAGCT 
TTCTTTGACA GCATTGTAGG TGTAGCAGCA TATGTGGGCT GGCAGAGCTC CTCCATCCTG 
AAGCCTCTTC TCCAGCAAGG CCTGAGTGAC GTTGAAGAGT TTGTCATTTA TAAAGCTCTC 
AATGCTCTGA CCTGCATGTG TCAGCTGGGG CTGTTGCAGA AGCCTCACAT CTATGAGTTT 
GTCAGCGATA TTGCTCCATT CTTGTGCCAC CCCAATCTGT GGATCCGCTA TGGAGCTGTG 
GGTTTCATCA CTGTCGTCGC TCAGCACCTA AACATCGCTG ATGTTTACTG CAAACTTATG 
CCACATCTTA ACCCTTTCAT CACACAACCC ATTATACAGA TTGATAAGGA GATTGTGTTG 
CTCAGTGTGC TGAAAGAGCC TGTAAGTCGC TCCATCTTTG ACTATGCACT GCGTTCCAAA 
GACATCGGAA GCCTTTTCAG ACACCTGTTA CTCCGGCAAA AGAAGCGCAA CGGCTCCATC 
CCAGAATGCC CCATTCCTGA GGATCCTGCA ATTGCCCAGC TTCTTAAAAA ACTTCTCTCA 
CAGGGCATGA CAGAGGCTGA AGAGGATAAG CTCTTGGCTC TGAAAGATTT CATGCTCAAG 
TCTAACAAGG CCAAAGCCAA CATCATAGAC CAGAGCCACC TGAGTGAAGG AGCCCACAGT 
GGAGTCATTG ATCTCGGGAC ACTGGGCATC ACTGGCCGAC AGGTGGACCT GGTCAAGCCC 
AAGCAGGAGA TTGAAGATAA GAGAGAGTTC ATCTCTTCTG CCTCTGCTCG CAAACATACG 
AAACAGGACT CCAACTTGAA CGAAGAGTGG AAGAGCATGT TTGGTTCTCT GGATCCTCCC 
AGCTCGGCAT CCACGCCCTC GACGATTCCA GACAGTGGAG CCGCGCAGCC CCGGAAAACC 
ACAGCGCTTC CTACTATTCA AGTGGTTCAG TCGATAACTG GAGCGTCCAC ATATCAGCGG 
CGCATCACCA CATGTAAAGC AGAGCTGCAG CAGCTGGTGC AGCAGAAGAG AGAGCAGTGC 
AATGCAGAGC GCATGGCCAA ACAGATGATG GAGAGCGCAG AGTGGGAAAG CAGACCTCCA 
CTGCCAGGGT GGCATCCAAA AGGTCTTCTA GTGGCCCATC TACATGAGCA CAAATCTGCT 
GTCAACCGAA TCCGAGTCTC AGATGAGCAC TCCATCTTTG CTACCTGCTC CAATGATGGA 
ACTGTCAAAA TCTGGGACAG CCAAAAAATG GAGGGCAAGA CGACTACCAC GAGGTCTGTG 
CTGACATATT CTCGGATTGG AGGTCATGTG AAAACATTGA CATTTTGCCA GGGATCGCAT 
TACCTTGCAG TTGCATCAGA TAATGGTTCC ATCCAACTCC TTGCAGTCGA GGCAAACAAG 
CCTCCCAAAT CACCCAAAGT CCAGCCCTGT CAGACAAGGT TTTTGGATCC AAAGGAGGAG 
GGCTGTGTGG TGGACGTACA TCATTTTAAC TCTGGGGCGC AGACGGTTTT GGCCTACGCT 
ACTGTAAATG GCTTTTTGGT CGGTTGGGAC CTGCGAAGCA ACAGCAACGC ATGGACTCTT 
CGCCATGATC TGCGTCTGGG CCTCATCACT TCCTTTGCTG TTGATATGCA CCAGTGTTGG 
CTGTGTGTAG GCACAAGTAA TGGCACAATG GCATGCTGGG ATATGCGGTT TCAGCTACCA 
ATTTCCAGTC ACTCCCATCC AGCCCGAGCT CGGATCAGAC GCCTTCTGAT GCATCCACTC 
TATCAGTCAT CTGTCATTGC AGCCGTTCAG GGTAACAATG AGGTCTCCAT GTGGGATATG 
GAGACCGGAG ACAGGAAGTT CACCCTGTGG GCGAGCTCTG CGCCTCCGCT TTCGGAGATG 
CAGCCCTCTC CACACAGTGT ACATGGCATA TACTGCAGCC CTGCGGATGG AAACCCTCTC 
CTCCTCACAG CCGGATCTGA CATGAGAATC AGGTTTTGGG ATTTGGCTTA CCCTGAGAGA 
TCATACATCG TCGCAGGCGG TGCTAACGAC TCCCTCCACT GCCCTTCTGT TTTCTACAAC 
CGCAAGATCA TCGAAGGAAC AGAAGTGGTT CAGGAGATTC ACAGTAAACA GAAGAGCGGC 
TCCACCGAGG ATACTCCTCG CCGAGGCCCA GAATCCCTTC CAGTGGGCCA CCATGACATC 
ATAACAGACA TCGCCACTTT CCAGACCACA CAGGGCTTTA TAGTCACTTC CTCTAGAGAT 
GGCATTGTCA AAGTCTGGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322650.1
CDS	308..4351
Translation	MGNQLAGIAPSQILSVDSYFSDIHDYEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPSLPLTSYKQEVEELKIRLHSCQNCLPFQKATLTEKAAILFRQYVRDNLYDRISTRPFLNNVEKKWIAFQLLNAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGSMFATESDQTTPLVDLSSNSQRTRGELKQPMDIFSAGCVIAELFTEGVPLFDLSQLLAYRKGHFQTEQVLMKIEDHSIRELVAQMVQREPEKRLTAEEYLKQQRGKAFPEIFYTFLQPYMAQFAKETFQSADERVLVIRKNLENILNNLCSGGQTAKAEKQEKASQEFSSSKEQGLVVLVSVITSCLQTLRFCDSKLAALELILHLAGRLNVEILLDRITPYLLHFCNDSMPRVRAEAVRTLTKVLALVKEVPRNDVNIYPEYILPGIAHLAQDEATIVRLAYAENIAHLAETALRFLELVQENNLSSEHDLNGEDTEETLHPNENYDSELQALHEMVQQKVVTLLSDPENIVKQTLMENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDVEEFVIYKALNALTCMCQLGLLQKPHIYEFVSDIAPFLCHPNLWIRYGAVGFITVVAQHLNIADVYCKLMPHLNPFITQPIIQIDKEIVLLSVLKEPVSRSIFDYALRSKDIGSLFRHLLLRQKKRNGSIPECPIPEDPAIAQLLKKLLSQGMTEAEEDKLLALKDFMLKSNKAKANIIDQSHLSEGAHSGVIDLGTLGITGRQVDLVKPKQEIEDKREFISSASARKHTKQDSNLNEEWKSMFGSLDPPSSASTPSTIPDSGAAQPRKTTALPTIQVVQSITGASTYQRRITTCKAELQQLVQQKREQCNAERMAKQMMESAEWESRPPLPGWHPKGLLVAHLHEHKSAVNRIRVSDEHSIFATCSNDGTVKIWDSQKMEGKTTTTRSVLTYSRIGGHVKTLTFCQGSHYLAVASDNGSIQLLAVEANKPPKSPKVQPCQTRFLDPKEEGCVVDVHHFNSGAQTVLAYATVNGFLVGWDLRSNSNAWTLRHDLRLGLITSFAVDMHQCWLCVGTSNGTMACWDMRFQLPISSHSHPARARIRRLLMHPLYQSSVIAAVQGNNEVSMWDMETGDRKFTLWASSAPPLSEMQPSPHSVHGIYCSPADGNPLLLTAGSDMRIRFWDLAYPERSYIVAGGANDSLHCPSVFYNRKIIEGTEVVQEIHSKQKSGSTEDTPRRGPESLPVGHHDIITDIATFQTTQGFIVTSSRDGIVKVWK

Target ORF information:

RefSeq Version	XM_021466975.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466975.1

ORF Insert Sequence:

ATGGGGAACC AACTGGCTGG AATTGCTCCA TCGCAGATCC TGTCAGTGGA CAGTTACTTC 
TCTGACATCC ATGACTACGA ATATGACAAA AGTCTGGGCA GTACACGATT TTTCAAAGTG 
GCCCGGGCGA AACACAGGGA AGGATTGGTC GTTGTTAAGG TCTTTGCTAT CCAAGACCCT 
TCTCTACCCC TGACCAGCTA CAAACAAGAG GTGGAGGAGC TGAAAATCCG ACTGCACTCC 
TGTCAGAACT GCCTGCCTTT CCAAAAAGCC ACGCTTACTG AAAAAGCAGC CATTCTGTTT 
CGCCAGTATG TTCGGGATAA CTTGTATGAC CGTATTAGTA CGCGGCCTTT CCTCAACAAC 
GTGGAAAAGA AGTGGATCGC CTTCCAGCTC CTCAATGCTG TGGACCAGGC CCATAAATCG 
GGAGTGCGTC ACGGAGACAT TAAAACAGAG AACGTCATGG TGACAAGCTG GAACTGGGTG 
CTTCTAACAG ACTTTGCTAG CTTTAAGCCA ACATACTTGC CTGAAGACAA TCCCGCAGAT 
TTTAATTACT TCTTCGACAC GTCCAGGAGG AGAACATGTT ACATTGCACC TGAGAGATTT 
GTGGATGGCA GCATGTTTGC TACAGAAAGT GACCAGACCA CTCCTCTCGT AGATCTTTCT 
AGTAATAGTC AGAGGACCAG AGGGGAGCTC AAGCAGCCCA TGGACATCTT CTCAGCTGGT 
TGTGTGATTG CGGAATTGTT CACAGAGGGC GTCCCGCTCT TTGACCTCTC ACAACTGCTG 
GCTTATCGTA AAGGGCATTT CCAAACCGAA CAAGTGCTGA TGAAAATTGA AGATCACAGT 
ATTCGAGAAC TGGTGGCTCA AATGGTGCAG AGAGAGCCAG AAAAGCGACT GACAGCTGAA 
GAATACCTTA AGCAGCAGCG AGGAAAAGCT TTCCCAGAAA TCTTCTACAC CTTCCTTCAA 
CCGTACATGG CCCAGTTTGC CAAGGAGACC TTCCAGTCTG CTGACGAAAG AGTCTTGGTG 
ATCCGTAAAA ACCTGGAGAA CATCCTCAAC AACCTGTGTA GCGGCGGGCA GACGGCGAAG 
GCTGAAAAAC AGGAGAAAGC ATCCCAAGAG TTTAGCTCAT CTAAAGAACA AGGCCTGGTG 
GTGCTGGTGT CAGTGATCAC TTCATGTCTA CAGACGCTTC GATTTTGTGA CTCCAAGCTG 
GCGGCGCTGG AGCTGATCCT TCATCTGGCA GGCCGGCTCA ATGTGGAGAT TCTGCTGGAC 
AGGATTACGC CGTATTTGCT GCACTTCTGC AATGACTCTA TGCCGCGTGT CAGAGCTGAG 
GCCGTTCGCA CGCTGACTAA GGTGCTGGCG CTGGTCAAGG AGGTTCCCCG CAATGATGTC 
AACATCTACC CGGAGTACAT TTTGCCTGGC ATTGCCCACT TGGCCCAAGA TGAAGCCACG 
ATTGTCAGAC TTGCTTATGC AGAAAACATA GCCCATCTAG CCGAGACAGC ACTGCGTTTT 
CTGGAGCTGG TGCAGGAGAA TAATTTGAGC TCTGAACACG ATCTGAATGG AGAGGACACA 
GAAGAAACTC TTCATCCCAA TGAAAACTAT GATTCAGAGT TGCAGGCCTT GCATGAGATG 
GTTCAGCAGA AGGTGGTGAC TTTGCTGAGC GATCCTGAAA ACATCGTCAA ACAGACGCTG 
ATGGAGAATG GCATTACGCG CCTCTGCGTG TTCTTCGGCA GGCAGAAAGC CAATGACGTC 
CTTCTATCCC ACATGATCAC CTTCCTGAAT GACAAGAACG ACTGGCATCT CCGCGGAGCT 
TTCTTTGACA GCATTGTAGG TGTAGCAGCA TATGTGGGCT GGCAGAGCTC CTCCATCCTG 
AAGCCTCTTC TCCAGCAAGG CCTGAGTGAC GTTGAAGAGT TTGTCATTTA TAAAGCTCTC 
AATGCTCTGA CCTGCATGTG TCAGCTGGGG CTGTTGCAGA AGCCTCACAT CTATGAGTTT 
GTCAGCGATA TTGCTCCATT CTTGTGCCAC CCCAATCTGT GGATCCGCTA TGGAGCTGTG 
GGTTTCATCA CTGTCGTCGC TCAGCACCTA AACATCGCTG ATGTTTACTG CAAACTTATG 
CCACATCTTA ACCCTTTCAT CACACAACCC ATTATACAGA TTGATAAGGA GATTGTGTTG 
CTCAGTGTGC TGAAAGAGCC TGTAAGTCGC TCCATCTTTG ACTATGCACT GCGTTCCAAA 
GACATCGGAA GCCTTTTCAG ACACCTGTTA CTCCGGCAAA AGAAGCGCAA CGGCTCCATC 
CCAGAATGCC CCATTCCTGA GGATCCTGCA ATTGCCCAGC TTCTTAAAAA ACTTCTCTCA 
CAGGGCATGA CAGAGGCTGA AGAGGATAAG CTCTTGGCTC TGAAAGATTT CATGCTCAAG 
TCTAACAAGG CCAAAGCCAA CATCATAGAC CAGAGCCACC TGAGTGAAGG AGCCCACAGT 
GGAGTCATTG ATCTCGGGAC ACTGGGCATC ACTGGCCGAC AGGTGGACCT GGTCAAGCCC 
AAGCAGGAGA TTGAAGATAA GAGAGAGTTC ATCTCTTCTG CCTCTGCTCG CAAACATACG 
AAACAGGACT CCAACTTGAA CGAAGAGTGG AAGAGCATGT TTGGTTCTCT GGATCCTCCC 
AGCTCGGCAT CCACGCCCTC GACGATTCCA GACAGTGGAG CCGCGCAGCC CCGGAAAACC 
ACAGCGCTTC CTACTATTCA AGTGGTTCAG TCGATAACTG GAGCGTCCAC ATATCAGCGG 
CGCATCACCA CATGTAAAGC AGAGCTGCAG CAGCTGGTGC AGCAGAAGAG AGAGCAGTGC 
AATGCAGAGC GCATGGCCAA ACAGATGATG GAGAGCGCAG AGTGGGAAAG CAGACCTCCA 
CTGCCAGGGT GGCATCCAAA AGGTCTTCTA GTGGCCCATC TACATGAGCA CAAATCTGCT 
GTCAACCGAA TCCGAGTCTC AGATGAGCAC TCCATCTTTG CTACCTGCTC CAATGATGGA 
ACTGTCAAAA TCTGGGACAG CCAAAAAATG GAGGGCAAGA CGACTACCAC GAGGTCTGTG 
CTGACATATT CTCGGATTGG AGGTCATGTG AAAACATTGA CATTTTGCCA GGGATCGCAT 
TACCTTGCAG TTGCATCAGA TAATGGTTCC ATCCAACTCC TTGCAGTCGA GGCAAACAAG 
CCTCCCAAAT CACCCAAAGT CCAGCCCTGT CAGACAAGGT TTTTGGATCC AAAGGAGGAG 
GGCTGTGTGG TGGACGTACA TCATTTTAAC TCTGGGGCGC AGACGGTTTT GGCCTACGCT 
ACTGTAAATG GCTTTTTGGT CGGTTGGGAC CTGCGAAGCA ACAGCAACGC ATGGACTCTT 
CGCCATGATC TGCGTCTGGG CCTCATCACT TCCTTTGCTG TTGATATGCA CCAGTGTTGG 
CTGTGTGTAG GCACAAGTAA TGGCACAATG GCATGCTGGG ATATGCGGTT TCAGCTACCA 
ATTTCCAGTC ACTCCCATCC AGCCCGAGCT CGGATCAGAC GCCTTCTGAT GCATCCACTC 
TATCAGTCAT CTGTCATTGC AGCCGTTCAG GGTAACAATG AGGTCTCCAT GTGGGATATG 
GAGACCGGAG ACAGGAAGTT CACCCTGTGG GCGAGCTCTG CGCCTCCGCT TTCGGAGATG 
CAGCCCTCTC CACACAGTGT ACATGGCATA TACTGCAGCC CTGCGGATGG AAACCCTCTC 
CTCCTCACAG CCGGATCTGA CATGAGAATC AGGTTTTGGG ATTTGGCTTA CCCTGAGAGA 
TCATACATCG TCGCAGGCGG TGCTAACGAC TCCCTCCACT GCCCTTCTGT TTTCTACAAC 
CGCAAGATCA TCGAAGGAAC AGAAGTGGTT CAGGAGATTC ACAGTAAACA GAAGAGCGGC 
TCCACCGAGG ATACTCCTCG CCGAGGCCCA GAATCCCTTC CAGTGGGCCA CCATGACATC 
ATAACAGACA TCGCCACTTT CCAGACCACA CAGGGCTTTA TAGTCACTTC CTCTAGAGAT 
GGCATTGTCA AAGTCTGGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3R4 ( XM_001922641.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001922676.1 Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X1, mRNA.

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CloneID	ODa21700
Clone ID Related Accession (Same CDS sequence)	XM_001922641.5 , XM_001922641.6
Accession Version	XM_001922641.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	phosphoinositide 3-kinase regulatory subunit 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001922641.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAACC AACTGGCTGG AATTGCTCCA TCGCAGATCC TGTCAGTGGA CAGTTACTTC 
TCTGACATCC ATGACTACGA ATATGACAAA AGTCTGGGCA GTACACGATT TTTCAAAGTG 
GCCCGGGCGA AACACAGGGA AGGATTGGTC GTTGTTAAGG TCTTTGCTAT CCAAGACCCT 
TCTCTACCCC TGACCAGCTA CAAACAAGAG GTGGAGGAGC TGAAAATCCG ACTGCACTCC 
TGTCAGAACT GCCTGCCTTT CCAAAAAGCC ACGCTTACTG AAAAAGCAGC CATTCTGTTT 
CGCCAGTATG TTCGGGATAA CTTGTATGAC CGTATTAGTA CGCGGCCTTT CCTCAACAAC 
GTGGAAAAGA AGTGGATCGC CTTCCAGCTC CTCAATGCTG TGGACCAGGC CCATAAATCG 
GGAGTGCGTC ACGGAGACAT TAAAACAGAG AACGTCATGG TGACAAGCTG GAACTGGGTG 
CTTCTAACAG ACTTTGCTAG CTTTAAGCCA ACATACTTGC CTGAAGACAA TCCCGCAGAT 
TTTAATTACT TCTTCGACAC GTCCAGGAGG AGAACATGTT ACATTGCACC TGAGAGATTT 
GTGGATGGCA GCATGTTTGC TACAGAAAGT GACCAGACCA CTCCTCTCGT AGATCTTTCT 
AGTAATAGTC AGAGGACCAG AGGGGAGCTC AAGCAGCCCA TGGACATCTT CTCAGCTGGT 
TGTGTGATTG CGGAATTGTT CACAGAGGGC GTCCCGCTCT TTGACCTCTC ACAACTGCTG 
GCTTATCGTA AAGGGCATTT CCAAACCGAA CAAGTGCTGA TGAAAATTGA AGATCACAGT 
ATTCGAGAAC TGGTGGCTCA AATGGTGCAG AGAGAGCCAG AAAAGCGACT GACAGCTGAA 
GAATACCTTA AGCAGCAGCG AGGAAAAGCT TTCCCAGAAA TCTTCTACAC CTTCCTTCAA 
CCGTACATGG CCCAGTTTGC CAAGGAGACC TTCCAGTCTG CTGACGAAAG AGTCTTGGTG 
ATCCGTAAAA ACCTGGAGAA CATCCTCAAC AACCTGTGTA GCGGCGGGCA GACGGCGAAG 
GCTGAAAAAC AGGAGAAAGC ATCCCAAGAG TTTAGCTCAT CTAAAGAACA AGGCCTGGTG 
GTGCTGGTGT CAGTGATCAC TTCATGTCTA CAGACGCTTC GATTTTGTGA CTCCAAGCTG 
GCGGCGCTGG AGCTGATCCT TCATCTGGCA GGCCGGCTCA ATGTGGAGAT TCTGCTGGAC 
AGGATTACGC CGTATTTGCT GCACTTCTGC AATGACTCTA TGCCGCGTGT CAGAGCTGAG 
GCCGTTCGCA CGCTGACTAA GGTGCTGGCG CTGGTCAAGG AGGTTCCCCG CAATGATGTC 
AACATCTACC CGGAGTACAT TTTGCCTGGC ATTGCCCACT TGGCCCAAGA TGAAGCCACG 
ATTGTCAGAC TTGCTTATGC AGAAAACATA GCCCATCTAG CCGAGACAGC ACTGCGTTTT 
CTGGAGCTGG TGCAGGAGAA TAATTTGAGC TCTGAACACG ATCTGAATGG AGAGGACACA 
GAAGAAACTC TTCATCCCAA TGAAAACTAT GATTCAGAGT TGCAGGCCTT GCATGAGATG 
GTTCAGCAGA AGGTGGTGAC TTTGCTGAGC GATCCTGAAA ACATCGTCAA ACAGACGCTG 
ATGGAGAATG GCATTACGCG CCTCTGCGTG TTCTTCGGCA GGCAGAAAGC CAATGACGTC 
CTTCTATCCC ACATGATCAC CTTCCTGAAT GACAAGAACG ACTGGCATCT CCGCGGAGCT 
TTCTTTGACA GCATTGTAGG TGTAGCAGCA TATGTGGGCT GGCAGAGCTC CTCCATCCTG 
AAGCCTCTTC TCCAGCAAGG CCTGAGTGAC GTTGAAGAGT TTGTCATTTA TAAAGCTCTC 
AATGCTCTGA CCTGCATGTG TCAGCTGGGG CTGTTGCAGA AGCCTCACAT CTATGAGTTT 
GTCAGCGATA TTGCTCCATT CTTGTGCCAC CCCAATCTGT GGATCCGCTA TGGAGCTGTG 
GGTTTCATCA CTGTCGTCGC TCAGCACCTA AACATCGCTG ATGTTTACTG CAAACTTATG 
CCACATCTTA ACCCTTTCAT CACACAACCC ATTATACAGA TTGATAAGGA GATTGTGTTG 
CTCAGTGTGC TGAAAGAGCC TGTAAGTCGC TCCATCTTTG ACTATGCACT GCGTTCCAAA 
GACATCGGAA GCCTTTTCAG ACACCTGTTA CTCCGGCAAA AGAAGCGCAA CGGCTCCATC 
CCAGAATGCC CCATTCCTGA GGATCCTGCA ATTGCCCAGC TTCTTAAAAA ACTTCTCTCA 
CAGGGCATGA CAGAGGCTGA AGAGGATAAG CTCTTGGCTC TGAAAGATTT CATGCTCAAG 
TCTAACAAGG CCAAAGCCAA CATCATAGAC CAGAGCCACC TGAGTGAAGG AGCCCACAGT 
GGAGTCATTG ATCTCGGGAC ACTGGGCATC ACTGGCCGAC AGGTGGACCT GGTCAAGCCC 
AAGCAGGAGA TTGAAGATAA GAGAGCTCGC AAACATACGA AACAGGACTC CAACTTGAAC 
GAAGAGTGGA AGAGCATGTT TGGTTCTCTG GATCCTCCCA GCTCGGCATC CACGCCCTCG 
ACGGTACCTA ATGTTGTAGT TGCGGGCGGT GGTGGTGGTG GTGGGGAGCC CAATGGAGCT 
CTGGCGGGAG AGCGCCTGCG ATCTGAGAGT TCCTCACAGA CTCTTAACCT CCCTCATGTT 
CCATCCTCAG CCCAGATTCC AGACAGTGGA GCCGCGCAGC CCCGGAAAAC CACAGCGCTT 
CCTACTATTC AAGTGGTTCA GTCGATAACT GGAGCGTCCA CATATCAGCG GCGCATCACC 
ACATGTAAAG CAGAGCTGCA GCAGCTGGTG CAGCAGAAGA GAGAGCAGTG CAATGCAGAG 
CGCATGGCCA AACAGATGAT GGAGAGCGCA GAGTGGGAAA GCAGACCTCC ACTGCCAGGG 
TGGCATCCAA AAGGTCTTCT AGTGGCCCAT CTACATGAGC ACAAATCTGC TGTCAACCGA 
ATCCGAGTCT CAGATGAGCA CTCCATCTTT GCTACCTGCT CCAATGATGG AACTGTCAAA 
ATCTGGGACA GCCAAAAAAT GGAGGGCAAG ACGACTACCA CGAGGTCTGT GCTGACATAT 
TCTCGGATTG GAGGTCATGT GAAAACATTG ACATTTTGCC AGGGATCGCA TTACCTTGCA 
GTTGCATCAG ATAATGGTTC CATCCAACTC CTTGCAGTCG AGGCAAACAA GCCTCCCAAA 
TCACCCAAAG TCCAGCCCTG TCAGACAAGG TTTTTGGATC CAAAGGAGGA GGGCTGTGTG 
GTGGACGTAC ATCATTTTAA CTCTGGGGCG CAGACGGTTT TGGCCTACGC TACTGTAAAT 
GGCTTTTTGG TCGGTTGGGA CCTGCGAAGC AACAGCAACG CATGGACTCT TCGCCATGAT 
CTGCGTCTGG GCCTCATCAC TTCCTTTGCT GTTGATATGC ACCAGTGTTG GCTGTGTGTA 
GGCACAAGTA ATGGCACAAT GGCATGCTGG GATATGCGGT TTCAGCTACC AATTTCCAGT 
CACTCCCATC CAGCCCGAGC TCGGATCAGA CGCCTTCTGA TGCATCCACT CTATCAGTCA 
TCTGTCATTG CAGCCGTTCA GGGTAACAAT GAGGTCTCCA TGTGGGATAT GGAGACCGGA 
GACAGGAAGT TCACCCTGTG GGCGAGCTCT GCGCCTCCGC TTTCGGAGAT GCAGCCCTCT 
CCACACAGTG TACATGGCAT ATACTGCAGC CCTGCGGATG GAAACCCTCT CCTCCTCACA 
GCCGGATCTG ACATGAGAAT CAGGTTTTGG GATTTGGCTT ACCCTGAGAG ATCATACATC 
GTCGCAGGCG GTGCTAACGA CTCCCTCCAC TGCCCTTCTG TTTTCTACAA CCGCAAGATC 
ATCGAAGGAA CAGAAGTGGT TCAGGAGATT CACAGTAAAC AGAAGAGCGG CTCCACCGAG 
GATACTCCTC GCCGAGGCCC AGAATCCCTT CCAGTGGGCC ACCATGACAT CATAACAGAC 
ATCGCCACTT TCCAGACCAC ACAGGGCTTT ATAGTCACTT CCTCTAGAGA TGGCATTGTC 
AAAGTCTGGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922676.1
CDS	313..4467
Translation	MGNQLAGIAPSQILSVDSYFSDIHDYEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPSLPLTSYKQEVEELKIRLHSCQNCLPFQKATLTEKAAILFRQYVRDNLYDRISTRPFLNNVEKKWIAFQLLNAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGSMFATESDQTTPLVDLSSNSQRTRGELKQPMDIFSAGCVIAELFTEGVPLFDLSQLLAYRKGHFQTEQVLMKIEDHSIRELVAQMVQREPEKRLTAEEYLKQQRGKAFPEIFYTFLQPYMAQFAKETFQSADERVLVIRKNLENILNNLCSGGQTAKAEKQEKASQEFSSSKEQGLVVLVSVITSCLQTLRFCDSKLAALELILHLAGRLNVEILLDRITPYLLHFCNDSMPRVRAEAVRTLTKVLALVKEVPRNDVNIYPEYILPGIAHLAQDEATIVRLAYAENIAHLAETALRFLELVQENNLSSEHDLNGEDTEETLHPNENYDSELQALHEMVQQKVVTLLSDPENIVKQTLMENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDVEEFVIYKALNALTCMCQLGLLQKPHIYEFVSDIAPFLCHPNLWIRYGAVGFITVVAQHLNIADVYCKLMPHLNPFITQPIIQIDKEIVLLSVLKEPVSRSIFDYALRSKDIGSLFRHLLLRQKKRNGSIPECPIPEDPAIAQLLKKLLSQGMTEAEEDKLLALKDFMLKSNKAKANIIDQSHLSEGAHSGVIDLGTLGITGRQVDLVKPKQEIEDKRARKHTKQDSNLNEEWKSMFGSLDPPSSASTPSTVPNVVVAGGGGGGGEPNGALAGERLRSESSSQTLNLPHVPSSAQIPDSGAAQPRKTTALPTIQVVQSITGASTYQRRITTCKAELQQLVQQKREQCNAERMAKQMMESAEWESRPPLPGWHPKGLLVAHLHEHKSAVNRIRVSDEHSIFATCSNDGTVKIWDSQKMEGKTTTTRSVLTYSRIGGHVKTLTFCQGSHYLAVASDNGSIQLLAVEANKPPKSPKVQPCQTRFLDPKEEGCVVDVHHFNSGAQTVLAYATVNGFLVGWDLRSNSNAWTLRHDLRLGLITSFAVDMHQCWLCVGTSNGTMACWDMRFQLPISSHSHPARARIRRLLMHPLYQSSVIAAVQGNNEVSMWDMETGDRKFTLWASSAPPLSEMQPSPHSVHGIYCSPADGNPLLLTAGSDMRIRFWDLAYPERSYIVAGGANDSLHCPSVFYNRKIIEGTEVVQEIHSKQKSGSTEDTPRRGPESLPVGHHDIITDIATFQTTQGFIVTSSRDGIVKVWK

Target ORF information:

RefSeq Version	XM_001922641.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922641.5

ORF Insert Sequence:

ATGGGGAACC AACTGGCTGG AATTGCTCCA TCGCAGATCC TGTCAGTGGA CAGTTACTTC 
TCTGACATCC ATGACTACGA ATATGACAAA AGTCTGGGCA GTACACGATT TTTCAAAGTG 
GCCCGGGCGA AACACAGGGA AGGATTGGTC GTTGTTAAGG TCTTTGCTAT CCAAGACCCT 
TCTCTACCCC TGACCAGCTA CAAACAAGAG GTGGAGGAGC TGAAAATCCG ACTGCACTCC 
TGTCAGAACT GCCTGCCTTT CCAAAAAGCC ACGCTTACTG AAAAAGCAGC CATTCTGTTT 
CGCCAGTATG TTCGGGATAA CTTGTATGAC CGTATTAGTA CGCGGCCTTT CCTCAACAAC 
GTGGAAAAGA AGTGGATCGC CTTCCAGCTC CTCAATGCTG TGGACCAGGC CCATAAATCG 
GGAGTGCGTC ACGGAGACAT TAAAACAGAG AACGTCATGG TGACAAGCTG GAACTGGGTG 
CTTCTAACAG ACTTTGCTAG CTTTAAGCCA ACATACTTGC CTGAAGACAA TCCCGCAGAT 
TTTAATTACT TCTTCGACAC GTCCAGGAGG AGAACATGTT ACATTGCACC TGAGAGATTT 
GTGGATGGCA GCATGTTTGC TACAGAAAGT GACCAGACCA CTCCTCTCGT AGATCTTTCT 
AGTAATAGTC AGAGGACCAG AGGGGAGCTC AAGCAGCCCA TGGACATCTT CTCAGCTGGT 
TGTGTGATTG CGGAATTGTT CACAGAGGGC GTCCCGCTCT TTGACCTCTC ACAACTGCTG 
GCTTATCGTA AAGGGCATTT CCAAACCGAA CAAGTGCTGA TGAAAATTGA AGATCACAGT 
ATTCGAGAAC TGGTGGCTCA AATGGTGCAG AGAGAGCCAG AAAAGCGACT GACAGCTGAA 
GAATACCTTA AGCAGCAGCG AGGAAAAGCT TTCCCAGAAA TCTTCTACAC CTTCCTTCAA 
CCGTACATGG CCCAGTTTGC CAAGGAGACC TTCCAGTCTG CTGACGAAAG AGTCTTGGTG 
ATCCGTAAAA ACCTGGAGAA CATCCTCAAC AACCTGTGTA GCGGCGGGCA GACGGCGAAG 
GCTGAAAAAC AGGAGAAAGC ATCCCAAGAG TTTAGCTCAT CTAAAGAACA AGGCCTGGTG 
GTGCTGGTGT CAGTGATCAC TTCATGTCTA CAGACGCTTC GATTTTGTGA CTCCAAGCTG 
GCGGCGCTGG AGCTGATCCT TCATCTGGCA GGCCGGCTCA ATGTGGAGAT TCTGCTGGAC 
AGGATTACGC CGTATTTGCT GCACTTCTGC AATGACTCTA TGCCGCGTGT CAGAGCTGAG 
GCCGTTCGCA CGCTGACTAA GGTGCTGGCG CTGGTCAAGG AGGTTCCCCG CAATGATGTC 
AACATCTACC CGGAGTACAT TTTGCCTGGC ATTGCCCACT TGGCCCAAGA TGAAGCCACG 
ATTGTCAGAC TTGCTTATGC AGAAAACATA GCCCATCTAG CCGAGACAGC ACTGCGTTTT 
CTGGAGCTGG TGCAGGAGAA TAATTTGAGC TCTGAACACG ATCTGAATGG AGAGGACACA 
GAAGAAACTC TTCATCCCAA TGAAAACTAT GATTCAGAGT TGCAGGCCTT GCATGAGATG 
GTTCAGCAGA AGGTGGTGAC TTTGCTGAGC GATCCTGAAA ACATCGTCAA ACAGACGCTG 
ATGGAGAATG GCATTACGCG CCTCTGCGTG TTCTTCGGCA GGCAGAAAGC CAATGACGTC 
CTTCTATCCC ACATGATCAC CTTCCTGAAT GACAAGAACG ACTGGCATCT CCGCGGAGCT 
TTCTTTGACA GCATTGTAGG TGTAGCAGCA TATGTGGGCT GGCAGAGCTC CTCCATCCTG 
AAGCCTCTTC TCCAGCAAGG CCTGAGTGAC GTTGAAGAGT TTGTCATTTA TAAAGCTCTC 
AATGCTCTGA CCTGCATGTG TCAGCTGGGG CTGTTGCAGA AGCCTCACAT CTATGAGTTT 
GTCAGCGATA TTGCTCCATT CTTGTGCCAC CCCAATCTGT GGATCCGCTA TGGAGCTGTG 
GGTTTCATCA CTGTCGTCGC TCAGCACCTA AACATCGCTG ATGTTTACTG CAAACTTATG 
CCACATCTTA ACCCTTTCAT CACACAACCC ATTATACAGA TTGATAAGGA GATTGTGTTG 
CTCAGTGTGC TGAAAGAGCC TGTAAGTCGC TCCATCTTTG ACTATGCACT GCGTTCCAAA 
GACATCGGAA GCCTTTTCAG ACACCTGTTA CTCCGGCAAA AGAAGCGCAA CGGCTCCATC 
CCAGAATGCC CCATTCCTGA GGATCCTGCA ATTGCCCAGC TTCTTAAAAA ACTTCTCTCA 
CAGGGCATGA CAGAGGCTGA AGAGGATAAG CTCTTGGCTC TGAAAGATTT CATGCTCAAG 
TCTAACAAGG CCAAAGCCAA CATCATAGAC CAGAGCCACC TGAGTGAAGG AGCCCACAGT 
GGAGTCATTG ATCTCGGGAC ACTGGGCATC ACTGGCCGAC AGGTGGACCT GGTCAAGCCC 
AAGCAGGAGA TTGAAGATAA GAGAGCTCGC AAACATACGA AACAGGACTC CAACTTGAAC 
GAAGAGTGGA AGAGCATGTT TGGTTCTCTG GATCCTCCCA GCTCGGCATC CACGCCCTCG 
ACGGTACCTA ATGTTGTAGT TGCGGGCGGT GGTGGTGGTG GTGGGGAGCC CAATGGAGCT 
CTGGCGGGAG AGCGCCTGCG ATCTGAGAGT TCCTCACAGA CTCTTAACCT CCCTCATGTT 
CCATCCTCAG CCCAGATTCC AGACAGTGGA GCCGCGCAGC CCCGGAAAAC CACAGCGCTT 
CCTACTATTC AAGTGGTTCA GTCGATAACT GGAGCGTCCA CATATCAGCG GCGCATCACC 
ACATGTAAAG CAGAGCTGCA GCAGCTGGTG CAGCAGAAGA GAGAGCAGTG CAATGCAGAG 
CGCATGGCCA AACAGATGAT GGAGAGCGCA GAGTGGGAAA GCAGACCTCC ACTGCCAGGG 
TGGCATCCAA AAGGTCTTCT AGTGGCCCAT CTACATGAGC ACAAATCTGC TGTCAACCGA 
ATCCGAGTCT CAGATGAGCA CTCCATCTTT GCTACCTGCT CCAATGATGG AACTGTCAAA 
ATCTGGGACA GCCAAAAAAT GGAGGGCAAG ACGACTACCA CGAGGTCTGT GCTGACATAT 
TCTCGGATTG GAGGTCATGT GAAAACATTG ACATTTTGCC AGGGATCGCA TTACCTTGCA 
GTTGCATCAG ATAATGGTTC CATCCAACTC CTTGCAGTCG AGGCAAACAA GCCTCCCAAA 
TCACCCAAAG TCCAGCCCTG TCAGACAAGG TTTTTGGATC CAAAGGAGGA GGGCTGTGTG 
GTGGACGTAC ATCATTTTAA CTCTGGGGCG CAGACGGTTT TGGCCTACGC TACTGTAAAT 
GGCTTTTTGG TCGGTTGGGA CCTGCGAAGC AACAGCAACG CATGGACTCT TCGCCATGAT 
CTGCGTCTGG GCCTCATCAC TTCCTTTGCT GTTGATATGC ACCAGTGTTG GCTGTGTGTA 
GGCACAAGTA ATGGCACAAT GGCATGCTGG GATATGCGGT TTCAGCTACC AATTTCCAGT 
CACTCCCATC CAGCCCGAGC TCGGATCAGA CGCCTTCTGA TGCATCCACT CTATCAGTCA 
TCTGTCATTG CAGCCGTTCA GGGTAACAAT GAGGTCTCCA TGTGGGATAT GGAGACCGGA 
GACAGGAAGT TCACCCTGTG GGCGAGCTCT GCGCCTCCGC TTTCGGAGAT GCAGCCCTCT 
CCACACAGTG TACATGGCAT ATACTGCAGC CCTGCGGATG GAAACCCTCT CCTCCTCACA 
GCCGGATCTG ACATGAGAAT CAGGTTTTGG GATTTGGCTT ACCCTGAGAG ATCATACATC 
GTCGCAGGCG GTGCTAACGA CTCCCTCCAC TGCCCTTCTG TTTTCTACAA CCGCAAGATC 
ATCGAAGGAA CAGAAGTGGT TCAGGAGATT CACAGTAAAC AGAAGAGCGG CTCCACCGAG 
GATACTCCTC GCCGAGGCCC AGAATCCCTT CCAGTGGGCC ACCATGACAT CATAACAGAC 
ATCGCCACTT TCCAGACCAC ACAGGGCTTT ATAGTCACTT CCTCTAGAGA TGGCATTGTC 
AAAGTCTGGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3R4 ( XM_001922641.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001922676.1 Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa21700
Clone ID Related Accession (Same CDS sequence)	XM_001922641.5 , XM_001922641.6
Accession Version	XM_001922641.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	phosphoinositide 3-kinase regulatory subunit 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001922641.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAACC AACTGGCTGG AATTGCTCCA TCGCAGATCC TGTCAGTGGA CAGTTACTTC 
TCTGACATCC ATGACTACGA ATATGACAAA AGTCTGGGCA GTACACGATT TTTCAAAGTG 
GCCCGGGCGA AACACAGGGA AGGATTGGTC GTTGTTAAGG TCTTTGCTAT CCAAGACCCT 
TCTCTACCCC TGACCAGCTA CAAACAAGAG GTGGAGGAGC TGAAAATCCG ACTGCACTCC 
TGTCAGAACT GCCTGCCTTT CCAAAAAGCC ACGCTTACTG AAAAAGCAGC CATTCTGTTT 
CGCCAGTATG TTCGGGATAA CTTGTATGAC CGTATTAGTA CGCGGCCTTT CCTCAACAAC 
GTGGAAAAGA AGTGGATCGC CTTCCAGCTC CTCAATGCTG TGGACCAGGC CCATAAATCG 
GGAGTGCGTC ACGGAGACAT TAAAACAGAG AACGTCATGG TGACAAGCTG GAACTGGGTG 
CTTCTAACAG ACTTTGCTAG CTTTAAGCCA ACATACTTGC CTGAAGACAA TCCCGCAGAT 
TTTAATTACT TCTTCGACAC GTCCAGGAGG AGAACATGTT ACATTGCACC TGAGAGATTT 
GTGGATGGCA GCATGTTTGC TACAGAAAGT GACCAGACCA CTCCTCTCGT AGATCTTTCT 
AGTAATAGTC AGAGGACCAG AGGGGAGCTC AAGCAGCCCA TGGACATCTT CTCAGCTGGT 
TGTGTGATTG CGGAATTGTT CACAGAGGGC GTCCCGCTCT TTGACCTCTC ACAACTGCTG 
GCTTATCGTA AAGGGCATTT CCAAACCGAA CAAGTGCTGA TGAAAATTGA AGATCACAGT 
ATTCGAGAAC TGGTGGCTCA AATGGTGCAG AGAGAGCCAG AAAAGCGACT GACAGCTGAA 
GAATACCTTA AGCAGCAGCG AGGAAAAGCT TTCCCAGAAA TCTTCTACAC CTTCCTTCAA 
CCGTACATGG CCCAGTTTGC CAAGGAGACC TTCCAGTCTG CTGACGAAAG AGTCTTGGTG 
ATCCGTAAAA ACCTGGAGAA CATCCTCAAC AACCTGTGTA GCGGCGGGCA GACGGCGAAG 
GCTGAAAAAC AGGAGAAAGC ATCCCAAGAG TTTAGCTCAT CTAAAGAACA AGGCCTGGTG 
GTGCTGGTGT CAGTGATCAC TTCATGTCTA CAGACGCTTC GATTTTGTGA CTCCAAGCTG 
GCGGCGCTGG AGCTGATCCT TCATCTGGCA GGCCGGCTCA ATGTGGAGAT TCTGCTGGAC 
AGGATTACGC CGTATTTGCT GCACTTCTGC AATGACTCTA TGCCGCGTGT CAGAGCTGAG 
GCCGTTCGCA CGCTGACTAA GGTGCTGGCG CTGGTCAAGG AGGTTCCCCG CAATGATGTC 
AACATCTACC CGGAGTACAT TTTGCCTGGC ATTGCCCACT TGGCCCAAGA TGAAGCCACG 
ATTGTCAGAC TTGCTTATGC AGAAAACATA GCCCATCTAG CCGAGACAGC ACTGCGTTTT 
CTGGAGCTGG TGCAGGAGAA TAATTTGAGC TCTGAACACG ATCTGAATGG AGAGGACACA 
GAAGAAACTC TTCATCCCAA TGAAAACTAT GATTCAGAGT TGCAGGCCTT GCATGAGATG 
GTTCAGCAGA AGGTGGTGAC TTTGCTGAGC GATCCTGAAA ACATCGTCAA ACAGACGCTG 
ATGGAGAATG GCATTACGCG CCTCTGCGTG TTCTTCGGCA GGCAGAAAGC CAATGACGTC 
CTTCTATCCC ACATGATCAC CTTCCTGAAT GACAAGAACG ACTGGCATCT CCGCGGAGCT 
TTCTTTGACA GCATTGTAGG TGTAGCAGCA TATGTGGGCT GGCAGAGCTC CTCCATCCTG 
AAGCCTCTTC TCCAGCAAGG CCTGAGTGAC GTTGAAGAGT TTGTCATTTA TAAAGCTCTC 
AATGCTCTGA CCTGCATGTG TCAGCTGGGG CTGTTGCAGA AGCCTCACAT CTATGAGTTT 
GTCAGCGATA TTGCTCCATT CTTGTGCCAC CCCAATCTGT GGATCCGCTA TGGAGCTGTG 
GGTTTCATCA CTGTCGTCGC TCAGCACCTA AACATCGCTG ATGTTTACTG CAAACTTATG 
CCACATCTTA ACCCTTTCAT CACACAACCC ATTATACAGA TTGATAAGGA GATTGTGTTG 
CTCAGTGTGC TGAAAGAGCC TGTAAGTCGC TCCATCTTTG ACTATGCACT GCGTTCCAAA 
GACATCGGAA GCCTTTTCAG ACACCTGTTA CTCCGGCAAA AGAAGCGCAA CGGCTCCATC 
CCAGAATGCC CCATTCCTGA GGATCCTGCA ATTGCCCAGC TTCTTAAAAA ACTTCTCTCA 
CAGGGCATGA CAGAGGCTGA AGAGGATAAG CTCTTGGCTC TGAAAGATTT CATGCTCAAG 
TCTAACAAGG CCAAAGCCAA CATCATAGAC CAGAGCCACC TGAGTGAAGG AGCCCACAGT 
GGAGTCATTG ATCTCGGGAC ACTGGGCATC ACTGGCCGAC AGGTGGACCT GGTCAAGCCC 
AAGCAGGAGA TTGAAGATAA GAGAGCTCGC AAACATACGA AACAGGACTC CAACTTGAAC 
GAAGAGTGGA AGAGCATGTT TGGTTCTCTG GATCCTCCCA GCTCGGCATC CACGCCCTCG 
ACGGTACCTA ATGTTGTAGT TGCGGGCGGT GGTGGTGGTG GTGGGGAGCC CAATGGAGCT 
CTGGCGGGAG AGCGCCTGCG ATCTGAGAGT TCCTCACAGA CTCTTAACCT CCCTCATGTT 
CCATCCTCAG CCCAGATTCC AGACAGTGGA GCCGCGCAGC CCCGGAAAAC CACAGCGCTT 
CCTACTATTC AAGTGGTTCA GTCGATAACT GGAGCGTCCA CATATCAGCG GCGCATCACC 
ACATGTAAAG CAGAGCTGCA GCAGCTGGTG CAGCAGAAGA GAGAGCAGTG CAATGCAGAG 
CGCATGGCCA AACAGATGAT GGAGAGCGCA GAGTGGGAAA GCAGACCTCC ACTGCCAGGG 
TGGCATCCAA AAGGTCTTCT AGTGGCCCAT CTACATGAGC ACAAATCTGC TGTCAACCGA 
ATCCGAGTCT CAGATGAGCA CTCCATCTTT GCTACCTGCT CCAATGATGG AACTGTCAAA 
ATCTGGGACA GCCAAAAAAT GGAGGGCAAG ACGACTACCA CGAGGTCTGT GCTGACATAT 
TCTCGGATTG GAGGTCATGT GAAAACATTG ACATTTTGCC AGGGATCGCA TTACCTTGCA 
GTTGCATCAG ATAATGGTTC CATCCAACTC CTTGCAGTCG AGGCAAACAA GCCTCCCAAA 
TCACCCAAAG TCCAGCCCTG TCAGACAAGG TTTTTGGATC CAAAGGAGGA GGGCTGTGTG 
GTGGACGTAC ATCATTTTAA CTCTGGGGCG CAGACGGTTT TGGCCTACGC TACTGTAAAT 
GGCTTTTTGG TCGGTTGGGA CCTGCGAAGC AACAGCAACG CATGGACTCT TCGCCATGAT 
CTGCGTCTGG GCCTCATCAC TTCCTTTGCT GTTGATATGC ACCAGTGTTG GCTGTGTGTA 
GGCACAAGTA ATGGCACAAT GGCATGCTGG GATATGCGGT TTCAGCTACC AATTTCCAGT 
CACTCCCATC CAGCCCGAGC TCGGATCAGA CGCCTTCTGA TGCATCCACT CTATCAGTCA 
TCTGTCATTG CAGCCGTTCA GGGTAACAAT GAGGTCTCCA TGTGGGATAT GGAGACCGGA 
GACAGGAAGT TCACCCTGTG GGCGAGCTCT GCGCCTCCGC TTTCGGAGAT GCAGCCCTCT 
CCACACAGTG TACATGGCAT ATACTGCAGC CCTGCGGATG GAAACCCTCT CCTCCTCACA 
GCCGGATCTG ACATGAGAAT CAGGTTTTGG GATTTGGCTT ACCCTGAGAG ATCATACATC 
GTCGCAGGCG GTGCTAACGA CTCCCTCCAC TGCCCTTCTG TTTTCTACAA CCGCAAGATC 
ATCGAAGGAA CAGAAGTGGT TCAGGAGATT CACAGTAAAC AGAAGAGCGG CTCCACCGAG 
GATACTCCTC GCCGAGGCCC AGAATCCCTT CCAGTGGGCC ACCATGACAT CATAACAGAC 
ATCGCCACTT TCCAGACCAC ACAGGGCTTT ATAGTCACTT CCTCTAGAGA TGGCATTGTC 
AAAGTCTGGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922676.1
CDS	308..4462
Translation	MGNQLAGIAPSQILSVDSYFSDIHDYEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPSLPLTSYKQEVEELKIRLHSCQNCLPFQKATLTEKAAILFRQYVRDNLYDRISTRPFLNNVEKKWIAFQLLNAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGSMFATESDQTTPLVDLSSNSQRTRGELKQPMDIFSAGCVIAELFTEGVPLFDLSQLLAYRKGHFQTEQVLMKIEDHSIRELVAQMVQREPEKRLTAEEYLKQQRGKAFPEIFYTFLQPYMAQFAKETFQSADERVLVIRKNLENILNNLCSGGQTAKAEKQEKASQEFSSSKEQGLVVLVSVITSCLQTLRFCDSKLAALELILHLAGRLNVEILLDRITPYLLHFCNDSMPRVRAEAVRTLTKVLALVKEVPRNDVNIYPEYILPGIAHLAQDEATIVRLAYAENIAHLAETALRFLELVQENNLSSEHDLNGEDTEETLHPNENYDSELQALHEMVQQKVVTLLSDPENIVKQTLMENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDVEEFVIYKALNALTCMCQLGLLQKPHIYEFVSDIAPFLCHPNLWIRYGAVGFITVVAQHLNIADVYCKLMPHLNPFITQPIIQIDKEIVLLSVLKEPVSRSIFDYALRSKDIGSLFRHLLLRQKKRNGSIPECPIPEDPAIAQLLKKLLSQGMTEAEEDKLLALKDFMLKSNKAKANIIDQSHLSEGAHSGVIDLGTLGITGRQVDLVKPKQEIEDKRARKHTKQDSNLNEEWKSMFGSLDPPSSASTPSTVPNVVVAGGGGGGGEPNGALAGERLRSESSSQTLNLPHVPSSAQIPDSGAAQPRKTTALPTIQVVQSITGASTYQRRITTCKAELQQLVQQKREQCNAERMAKQMMESAEWESRPPLPGWHPKGLLVAHLHEHKSAVNRIRVSDEHSIFATCSNDGTVKIWDSQKMEGKTTTTRSVLTYSRIGGHVKTLTFCQGSHYLAVASDNGSIQLLAVEANKPPKSPKVQPCQTRFLDPKEEGCVVDVHHFNSGAQTVLAYATVNGFLVGWDLRSNSNAWTLRHDLRLGLITSFAVDMHQCWLCVGTSNGTMACWDMRFQLPISSHSHPARARIRRLLMHPLYQSSVIAAVQGNNEVSMWDMETGDRKFTLWASSAPPLSEMQPSPHSVHGIYCSPADGNPLLLTAGSDMRIRFWDLAYPERSYIVAGGANDSLHCPSVFYNRKIIEGTEVVQEIHSKQKSGSTEDTPRRGPESLPVGHHDIITDIATFQTTQGFIVTSSRDGIVKVWK

Target ORF information:

RefSeq Version	XM_001922641.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922641.6

ORF Insert Sequence:

ATGGGGAACC AACTGGCTGG AATTGCTCCA TCGCAGATCC TGTCAGTGGA CAGTTACTTC 
TCTGACATCC ATGACTACGA ATATGACAAA AGTCTGGGCA GTACACGATT TTTCAAAGTG 
GCCCGGGCGA AACACAGGGA AGGATTGGTC GTTGTTAAGG TCTTTGCTAT CCAAGACCCT 
TCTCTACCCC TGACCAGCTA CAAACAAGAG GTGGAGGAGC TGAAAATCCG ACTGCACTCC 
TGTCAGAACT GCCTGCCTTT CCAAAAAGCC ACGCTTACTG AAAAAGCAGC CATTCTGTTT 
CGCCAGTATG TTCGGGATAA CTTGTATGAC CGTATTAGTA CGCGGCCTTT CCTCAACAAC 
GTGGAAAAGA AGTGGATCGC CTTCCAGCTC CTCAATGCTG TGGACCAGGC CCATAAATCG 
GGAGTGCGTC ACGGAGACAT TAAAACAGAG AACGTCATGG TGACAAGCTG GAACTGGGTG 
CTTCTAACAG ACTTTGCTAG CTTTAAGCCA ACATACTTGC CTGAAGACAA TCCCGCAGAT 
TTTAATTACT TCTTCGACAC GTCCAGGAGG AGAACATGTT ACATTGCACC TGAGAGATTT 
GTGGATGGCA GCATGTTTGC TACAGAAAGT GACCAGACCA CTCCTCTCGT AGATCTTTCT 
AGTAATAGTC AGAGGACCAG AGGGGAGCTC AAGCAGCCCA TGGACATCTT CTCAGCTGGT 
TGTGTGATTG CGGAATTGTT CACAGAGGGC GTCCCGCTCT TTGACCTCTC ACAACTGCTG 
GCTTATCGTA AAGGGCATTT CCAAACCGAA CAAGTGCTGA TGAAAATTGA AGATCACAGT 
ATTCGAGAAC TGGTGGCTCA AATGGTGCAG AGAGAGCCAG AAAAGCGACT GACAGCTGAA 
GAATACCTTA AGCAGCAGCG AGGAAAAGCT TTCCCAGAAA TCTTCTACAC CTTCCTTCAA 
CCGTACATGG CCCAGTTTGC CAAGGAGACC TTCCAGTCTG CTGACGAAAG AGTCTTGGTG 
ATCCGTAAAA ACCTGGAGAA CATCCTCAAC AACCTGTGTA GCGGCGGGCA GACGGCGAAG 
GCTGAAAAAC AGGAGAAAGC ATCCCAAGAG TTTAGCTCAT CTAAAGAACA AGGCCTGGTG 
GTGCTGGTGT CAGTGATCAC TTCATGTCTA CAGACGCTTC GATTTTGTGA CTCCAAGCTG 
GCGGCGCTGG AGCTGATCCT TCATCTGGCA GGCCGGCTCA ATGTGGAGAT TCTGCTGGAC 
AGGATTACGC CGTATTTGCT GCACTTCTGC AATGACTCTA TGCCGCGTGT CAGAGCTGAG 
GCCGTTCGCA CGCTGACTAA GGTGCTGGCG CTGGTCAAGG AGGTTCCCCG CAATGATGTC 
AACATCTACC CGGAGTACAT TTTGCCTGGC ATTGCCCACT TGGCCCAAGA TGAAGCCACG 
ATTGTCAGAC TTGCTTATGC AGAAAACATA GCCCATCTAG CCGAGACAGC ACTGCGTTTT 
CTGGAGCTGG TGCAGGAGAA TAATTTGAGC TCTGAACACG ATCTGAATGG AGAGGACACA 
GAAGAAACTC TTCATCCCAA TGAAAACTAT GATTCAGAGT TGCAGGCCTT GCATGAGATG 
GTTCAGCAGA AGGTGGTGAC TTTGCTGAGC GATCCTGAAA ACATCGTCAA ACAGACGCTG 
ATGGAGAATG GCATTACGCG CCTCTGCGTG TTCTTCGGCA GGCAGAAAGC CAATGACGTC 
CTTCTATCCC ACATGATCAC CTTCCTGAAT GACAAGAACG ACTGGCATCT CCGCGGAGCT 
TTCTTTGACA GCATTGTAGG TGTAGCAGCA TATGTGGGCT GGCAGAGCTC CTCCATCCTG 
AAGCCTCTTC TCCAGCAAGG CCTGAGTGAC GTTGAAGAGT TTGTCATTTA TAAAGCTCTC 
AATGCTCTGA CCTGCATGTG TCAGCTGGGG CTGTTGCAGA AGCCTCACAT CTATGAGTTT 
GTCAGCGATA TTGCTCCATT CTTGTGCCAC CCCAATCTGT GGATCCGCTA TGGAGCTGTG 
GGTTTCATCA CTGTCGTCGC TCAGCACCTA AACATCGCTG ATGTTTACTG CAAACTTATG 
CCACATCTTA ACCCTTTCAT CACACAACCC ATTATACAGA TTGATAAGGA GATTGTGTTG 
CTCAGTGTGC TGAAAGAGCC TGTAAGTCGC TCCATCTTTG ACTATGCACT GCGTTCCAAA 
GACATCGGAA GCCTTTTCAG ACACCTGTTA CTCCGGCAAA AGAAGCGCAA CGGCTCCATC 
CCAGAATGCC CCATTCCTGA GGATCCTGCA ATTGCCCAGC TTCTTAAAAA ACTTCTCTCA 
CAGGGCATGA CAGAGGCTGA AGAGGATAAG CTCTTGGCTC TGAAAGATTT CATGCTCAAG 
TCTAACAAGG CCAAAGCCAA CATCATAGAC CAGAGCCACC TGAGTGAAGG AGCCCACAGT 
GGAGTCATTG ATCTCGGGAC ACTGGGCATC ACTGGCCGAC AGGTGGACCT GGTCAAGCCC 
AAGCAGGAGA TTGAAGATAA GAGAGCTCGC AAACATACGA AACAGGACTC CAACTTGAAC 
GAAGAGTGGA AGAGCATGTT TGGTTCTCTG GATCCTCCCA GCTCGGCATC CACGCCCTCG 
ACGGTACCTA ATGTTGTAGT TGCGGGCGGT GGTGGTGGTG GTGGGGAGCC CAATGGAGCT 
CTGGCGGGAG AGCGCCTGCG ATCTGAGAGT TCCTCACAGA CTCTTAACCT CCCTCATGTT 
CCATCCTCAG CCCAGATTCC AGACAGTGGA GCCGCGCAGC CCCGGAAAAC CACAGCGCTT 
CCTACTATTC AAGTGGTTCA GTCGATAACT GGAGCGTCCA CATATCAGCG GCGCATCACC 
ACATGTAAAG CAGAGCTGCA GCAGCTGGTG CAGCAGAAGA GAGAGCAGTG CAATGCAGAG 
CGCATGGCCA AACAGATGAT GGAGAGCGCA GAGTGGGAAA GCAGACCTCC ACTGCCAGGG 
TGGCATCCAA AAGGTCTTCT AGTGGCCCAT CTACATGAGC ACAAATCTGC TGTCAACCGA 
ATCCGAGTCT CAGATGAGCA CTCCATCTTT GCTACCTGCT CCAATGATGG AACTGTCAAA 
ATCTGGGACA GCCAAAAAAT GGAGGGCAAG ACGACTACCA CGAGGTCTGT GCTGACATAT 
TCTCGGATTG GAGGTCATGT GAAAACATTG ACATTTTGCC AGGGATCGCA TTACCTTGCA 
GTTGCATCAG ATAATGGTTC CATCCAACTC CTTGCAGTCG AGGCAAACAA GCCTCCCAAA 
TCACCCAAAG TCCAGCCCTG TCAGACAAGG TTTTTGGATC CAAAGGAGGA GGGCTGTGTG 
GTGGACGTAC ATCATTTTAA CTCTGGGGCG CAGACGGTTT TGGCCTACGC TACTGTAAAT 
GGCTTTTTGG TCGGTTGGGA CCTGCGAAGC AACAGCAACG CATGGACTCT TCGCCATGAT 
CTGCGTCTGG GCCTCATCAC TTCCTTTGCT GTTGATATGC ACCAGTGTTG GCTGTGTGTA 
GGCACAAGTA ATGGCACAAT GGCATGCTGG GATATGCGGT TTCAGCTACC AATTTCCAGT 
CACTCCCATC CAGCCCGAGC TCGGATCAGA CGCCTTCTGA TGCATCCACT CTATCAGTCA 
TCTGTCATTG CAGCCGTTCA GGGTAACAAT GAGGTCTCCA TGTGGGATAT GGAGACCGGA 
GACAGGAAGT TCACCCTGTG GGCGAGCTCT GCGCCTCCGC TTTCGGAGAT GCAGCCCTCT 
CCACACAGTG TACATGGCAT ATACTGCAGC CCTGCGGATG GAAACCCTCT CCTCCTCACA 
GCCGGATCTG ACATGAGAAT CAGGTTTTGG GATTTGGCTT ACCCTGAGAG ATCATACATC 
GTCGCAGGCG GTGCTAACGA CTCCCTCCAC TGCCCTTCTG TTTTCTACAA CCGCAAGATC 
ATCGAAGGAA CAGAAGTGGT TCAGGAGATT CACAGTAAAC AGAAGAGCGG CTCCACCGAG 
GATACTCCTC GCCGAGGCCC AGAATCCCTT CCAGTGGGCC ACCATGACAT CATAACAGAC 
ATCGCCACTT TCCAGACCAC ACAGGGCTTT ATAGTCACTT CCTCTAGAGA TGGCATTGTC 
AAAGTCTGGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3R4 ( XM_005158299.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005158356.1 Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa21701
Clone ID Related Accession (Same CDS sequence)	XM_005158299.4 , XM_005158299.3
Accession Version	XM_005158299.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	phosphoinositide 3-kinase regulatory subunit 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158299.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAACC AACTGGCTGG AATTGCTCCA TCGCAGATCC TGTCAGTGGA CAGTTACTTC 
TCTGACATCC ATGACTACGA ATATGACAAA AGTCTGGGCA GTACACGATT TTTCAAAGTG 
GCCCGGGCGA AACACAGGGA AGGATTGGTC GTTGTTAAGG TCTTTGCTAT CCAAGACCCT 
TCTCTACCCC TGACCAGCTA CAAACAAGAG GTGGAGGAGC TGAAAATCCG ACTGCACTCC 
TGTCAGAACT GCCTGCCTTT CCAAAAAGCC ACGCTTACTG AAAAAGCAGC CATTCTGTTT 
CGCCAGTATG TTCGGGATAA CTTGTATGAC CGTATTAGTA CGCGGCCTTT CCTCAACAAC 
GTGGAAAAGA AGTGGATCGC CTTCCAGCTC CTCAATGCTG TGGACCAGGC CCATAAATCG 
GGAGTGCGTC ACGGAGACAT TAAAACAGAG AACGTCATGG TGACAAGCTG GAACTGGGTG 
CTTCTAACAG ACTTTGCTAG CTTTAAGCCA ACATACTTGC CTGAAGACAA TCCCGCAGAT 
TTTAATTACT TCTTCGACAC GTCCAGGAGG AGAACATGTT ACATTGCACC TGAGAGATTT 
GTGGATGGCA GCATGTTTGC TACAGAAAGT GACCAGACCA CTCCTCTCGT AGATCTTTCT 
AGTAATAGTC AGAGGACCAG AGGGGAGCTC AAGCAGCCCA TGGACATCTT CTCAGCTGGT 
TGTGTGATTG CGGAATTGTT CACAGAGGGC GTCCCGCTCT TTGACCTCTC ACAACTGCTG 
GCTTATCGTA AAGGGCATTT CCAAACCGAA CAAGTGCTGA TGAAAATTGA AGATCACAGT 
ATTCGAGAAC TGGTGGCTCA AATGGTGCAG AGAGAGCCAG AAAAGCGACT GACAGCTGAA 
GAATACCTTA AGCAGCAGCG AGGAAAAGCT TTCCCAGAAA TCTTCTACAC CTTCCTTCAA 
CCGTACATGG CCCAGTTTGC CAAGGAGACC TTCCAGTCTG CTGACGAAAG AGTCTTGGTG 
ATCCGTAAAA ACCTGGAGAA CATCCTCAAC AACCTGTGTA GCGGCGGGCA GACGGCGAAG 
GCTGAAAAAC AGGAGAAAGC ATCCCAAGAG TTTAGCTCAT CTAAAGAACA AGGCCTGGTG 
GTGCTGGTGT CAGTGATCAC TTCATGTCTA CAGACGCTTC GATTTTGTGA CTCCAAGCTG 
GCGGCGCTGG AGCTGATCCT TCATCTGGCA GGCCGGCTCA ATGTGGAGAT TCTGCTGGAC 
AGGATTACGC CGTATTTGCT GCACTTCTGC AATGACTCTA TGCCGCGTGT CAGAGCTGAG 
GCCGTTCGCA CGCTGACTAA GGTGCTGGCG CTGGTCAAGG AGGTTCCCCG CAATGATGTC 
AACATCTACC CGGAGTACAT TTTGCCTGGC ATTGCCCACT TGGCCCAAGA TGAAGCCACG 
ATTGTCAGAC TTGCTTATGC AGAAAACATA GCCCATCTAG CCGAGACAGC ACTGCGTTTT 
CTGGAGCTGG TGCAGGAGAA TAATTTGAGC TCTGAACACG ATCTGAATGG AGAGGACACA 
GAAGAAACTC TTCATCCCAA TGAAAACTAT GATTCAGAGT TGCAGGCCTT GCATGAGATG 
GTTCAGCAGA AGGTGGTGAC TTTGCTGAGC GATCCTGAAA ACATCGTCAA ACAGACGCTG 
ATGGAGAATG GCATTACGCG CCTCTGCGTG TTCTTCGGCA GGCAGAAAGC CAATGACGTC 
CTTCTATCCC ACATGATCAC CTTCCTGAAT GACAAGAACG ACTGGCATCT CCGCGGAGCT 
TTCTTTGACA GCATTGTAGG TGTAGCAGCA TATGTGGGCT GGCAGAGCTC CTCCATCCTG 
AAGCCTCTTC TCCAGCAAGG CCTGAGTGAC GTTGAAGAGT TTGTCATTTA TAAAGCTCTC 
AATGCTCTGA CCTGCATGTG TCAGCTGGGG CTGTTGCAGA AGCCTCACAT CTATGAGTTT 
GTCAGCGATA TTGCTCCATT CTTGTGCCAC CCCAATCTGT GGATCCGCTA TGGAGCTGTG 
GGTTTCATCA CTGTCGTCGC TCAGCACCTA AACATCGCTG ATGTTTACTG CAAACTTATG 
CCACATCTTA ACCCTTTCAT CACACAACCC ATTATACAGA TTGATAAGGA GATTGTGTTG 
CTCAGTGTGC TGAAAGAGCC TGTAAGTCGC TCCATCTTTG ACTATGCACT GCGTTCCAAA 
GACATCGGAA GCCTTTTCAG ACACCTGTTA CTCCGGCAAA AGAAGCGCAA CGGCTCCATC 
CCAGAATGCC CCATTCCTGA GGATCCTGCA ATTGCCCAGC TTCTTAAAAA ACTTCTCTCA 
CAGGGCATGA CAGAGGCTGA AGAGGATAAG CTCTTGGCTC TGAAAGATTT CATGCTCAAG 
TCTAACAAGG CCAAAGCCAA CATCATAGAC CAGAGCCACC TGAGTGAAGG AGCCCACAGT 
GGAGTCATTG ATCTCGGGAC ACTGGGCATC ACTGGCCGAC AGGTGGACCT GGTCAAGCCC 
AAGCAGGAGA TTGAAGATAA GAGAGCTCGC AAACATACGA AACAGGACTC CAACTTGAAC 
GAAGAGTGGA AGAGCATGTT TGGTTCTCTG GATCCTCCCA GCTCGGCATC CACGCCCTCG 
ACGATTCCAG ACAGTGGAGC CGCGCAGCCC CGGAAAACCA CAGCGCTTCC TACTATTCAA 
GTGGTTCAGT CGATAACTGG AGCGTCCACA TATCAGCGGC GCATCACCAC ATGTAAAGCA 
GAGCTGCAGC AGCTGGTGCA GCAGAAGAGA GAGCAGTGCA ATGCAGAGCG CATGGCCAAA 
CAGATGATGG AGAGCGCAGA GTGGGAAAGC AGACCTCCAC TGCCAGGGTG GCATCCAAAA 
GGTCTTCTAG TGGCCCATCT ACATGAGCAC AAATCTGCTG TCAACCGAAT CCGAGTCTCA 
GATGAGCACT CCATCTTTGC TACCTGCTCC AATGATGGAA CTGTCAAAAT CTGGGACAGC 
CAAAAAATGG AGGGCAAGAC GACTACCACG AGGTCTGTGC TGACATATTC TCGGATTGGA 
GGTCATGTGA AAACATTGAC ATTTTGCCAG GGATCGCATT ACCTTGCAGT TGCATCAGAT 
AATGGTTCCA TCCAACTCCT TGCAGTCGAG GCAAACAAGC CTCCCAAATC ACCCAAAGTC 
CAGCCCTGTC AGACAAGGTT TTTGGATCCA AAGGAGGAGG GCTGTGTGGT GGACGTACAT 
CATTTTAACT CTGGGGCGCA GACGGTTTTG GCCTACGCTA CTGTAAATGG CTTTTTGGTC 
GGTTGGGACC TGCGAAGCAA CAGCAACGCA TGGACTCTTC GCCATGATCT GCGTCTGGGC 
CTCATCACTT CCTTTGCTGT TGATATGCAC CAGTGTTGGC TGTGTGTAGG CACAAGTAAT 
GGCACAATGG CATGCTGGGA TATGCGGTTT CAGCTACCAA TTTCCAGTCA CTCCCATCCA 
GCCCGAGCTC GGATCAGACG CCTTCTGATG CATCCACTCT ATCAGTCATC TGTCATTGCA 
GCCGTTCAGG GTAACAATGA GGTCTCCATG TGGGATATGG AGACCGGAGA CAGGAAGTTC 
ACCCTGTGGG CGAGCTCTGC GCCTCCGCTT TCGGAGATGC AGCCCTCTCC ACACAGTGTA 
CATGGCATAT ACTGCAGCCC TGCGGATGGA AACCCTCTCC TCCTCACAGC CGGATCTGAC 
ATGAGAATCA GGTTTTGGGA TTTGGCTTAC CCTGAGAGAT CATACATCGT CGCAGGCGGT 
GCTAACGACT CCCTCCACTG CCCTTCTGTT TTCTACAACC GCAAGATCAT CGAAGGAACA 
GAAGTGGTTC AGGAGATTCA CAGTAAACAG AAGAGCGGCT CCACCGAGGA TACTCCTCGC 
CGAGGCCCAG AATCCCTTCC AGTGGGCCAC CATGACATCA TAACAGACAT CGCCACTTTC 
CAGACCACAC AGGGCTTTAT AGTCACTTCC TCTAGAGATG GCATTGTCAA AGTCTGGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158356.1
CDS	308..4330
Translation	MGNQLAGIAPSQILSVDSYFSDIHDYEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPSLPLTSYKQEVEELKIRLHSCQNCLPFQKATLTEKAAILFRQYVRDNLYDRISTRPFLNNVEKKWIAFQLLNAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGSMFATESDQTTPLVDLSSNSQRTRGELKQPMDIFSAGCVIAELFTEGVPLFDLSQLLAYRKGHFQTEQVLMKIEDHSIRELVAQMVQREPEKRLTAEEYLKQQRGKAFPEIFYTFLQPYMAQFAKETFQSADERVLVIRKNLENILNNLCSGGQTAKAEKQEKASQEFSSSKEQGLVVLVSVITSCLQTLRFCDSKLAALELILHLAGRLNVEILLDRITPYLLHFCNDSMPRVRAEAVRTLTKVLALVKEVPRNDVNIYPEYILPGIAHLAQDEATIVRLAYAENIAHLAETALRFLELVQENNLSSEHDLNGEDTEETLHPNENYDSELQALHEMVQQKVVTLLSDPENIVKQTLMENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDVEEFVIYKALNALTCMCQLGLLQKPHIYEFVSDIAPFLCHPNLWIRYGAVGFITVVAQHLNIADVYCKLMPHLNPFITQPIIQIDKEIVLLSVLKEPVSRSIFDYALRSKDIGSLFRHLLLRQKKRNGSIPECPIPEDPAIAQLLKKLLSQGMTEAEEDKLLALKDFMLKSNKAKANIIDQSHLSEGAHSGVIDLGTLGITGRQVDLVKPKQEIEDKRARKHTKQDSNLNEEWKSMFGSLDPPSSASTPSTIPDSGAAQPRKTTALPTIQVVQSITGASTYQRRITTCKAELQQLVQQKREQCNAERMAKQMMESAEWESRPPLPGWHPKGLLVAHLHEHKSAVNRIRVSDEHSIFATCSNDGTVKIWDSQKMEGKTTTTRSVLTYSRIGGHVKTLTFCQGSHYLAVASDNGSIQLLAVEANKPPKSPKVQPCQTRFLDPKEEGCVVDVHHFNSGAQTVLAYATVNGFLVGWDLRSNSNAWTLRHDLRLGLITSFAVDMHQCWLCVGTSNGTMACWDMRFQLPISSHSHPARARIRRLLMHPLYQSSVIAAVQGNNEVSMWDMETGDRKFTLWASSAPPLSEMQPSPHSVHGIYCSPADGNPLLLTAGSDMRIRFWDLAYPERSYIVAGGANDSLHCPSVFYNRKIIEGTEVVQEIHSKQKSGSTEDTPRRGPESLPVGHHDIITDIATFQTTQGFIVTSSRDGIVKVWK

Target ORF information:

RefSeq Version	XM_005158299.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158299.4

ORF Insert Sequence:

ATGGGGAACC AACTGGCTGG AATTGCTCCA TCGCAGATCC TGTCAGTGGA CAGTTACTTC 
TCTGACATCC ATGACTACGA ATATGACAAA AGTCTGGGCA GTACACGATT TTTCAAAGTG 
GCCCGGGCGA AACACAGGGA AGGATTGGTC GTTGTTAAGG TCTTTGCTAT CCAAGACCCT 
TCTCTACCCC TGACCAGCTA CAAACAAGAG GTGGAGGAGC TGAAAATCCG ACTGCACTCC 
TGTCAGAACT GCCTGCCTTT CCAAAAAGCC ACGCTTACTG AAAAAGCAGC CATTCTGTTT 
CGCCAGTATG TTCGGGATAA CTTGTATGAC CGTATTAGTA CGCGGCCTTT CCTCAACAAC 
GTGGAAAAGA AGTGGATCGC CTTCCAGCTC CTCAATGCTG TGGACCAGGC CCATAAATCG 
GGAGTGCGTC ACGGAGACAT TAAAACAGAG AACGTCATGG TGACAAGCTG GAACTGGGTG 
CTTCTAACAG ACTTTGCTAG CTTTAAGCCA ACATACTTGC CTGAAGACAA TCCCGCAGAT 
TTTAATTACT TCTTCGACAC GTCCAGGAGG AGAACATGTT ACATTGCACC TGAGAGATTT 
GTGGATGGCA GCATGTTTGC TACAGAAAGT GACCAGACCA CTCCTCTCGT AGATCTTTCT 
AGTAATAGTC AGAGGACCAG AGGGGAGCTC AAGCAGCCCA TGGACATCTT CTCAGCTGGT 
TGTGTGATTG CGGAATTGTT CACAGAGGGC GTCCCGCTCT TTGACCTCTC ACAACTGCTG 
GCTTATCGTA AAGGGCATTT CCAAACCGAA CAAGTGCTGA TGAAAATTGA AGATCACAGT 
ATTCGAGAAC TGGTGGCTCA AATGGTGCAG AGAGAGCCAG AAAAGCGACT GACAGCTGAA 
GAATACCTTA AGCAGCAGCG AGGAAAAGCT TTCCCAGAAA TCTTCTACAC CTTCCTTCAA 
CCGTACATGG CCCAGTTTGC CAAGGAGACC TTCCAGTCTG CTGACGAAAG AGTCTTGGTG 
ATCCGTAAAA ACCTGGAGAA CATCCTCAAC AACCTGTGTA GCGGCGGGCA GACGGCGAAG 
GCTGAAAAAC AGGAGAAAGC ATCCCAAGAG TTTAGCTCAT CTAAAGAACA AGGCCTGGTG 
GTGCTGGTGT CAGTGATCAC TTCATGTCTA CAGACGCTTC GATTTTGTGA CTCCAAGCTG 
GCGGCGCTGG AGCTGATCCT TCATCTGGCA GGCCGGCTCA ATGTGGAGAT TCTGCTGGAC 
AGGATTACGC CGTATTTGCT GCACTTCTGC AATGACTCTA TGCCGCGTGT CAGAGCTGAG 
GCCGTTCGCA CGCTGACTAA GGTGCTGGCG CTGGTCAAGG AGGTTCCCCG CAATGATGTC 
AACATCTACC CGGAGTACAT TTTGCCTGGC ATTGCCCACT TGGCCCAAGA TGAAGCCACG 
ATTGTCAGAC TTGCTTATGC AGAAAACATA GCCCATCTAG CCGAGACAGC ACTGCGTTTT 
CTGGAGCTGG TGCAGGAGAA TAATTTGAGC TCTGAACACG ATCTGAATGG AGAGGACACA 
GAAGAAACTC TTCATCCCAA TGAAAACTAT GATTCAGAGT TGCAGGCCTT GCATGAGATG 
GTTCAGCAGA AGGTGGTGAC TTTGCTGAGC GATCCTGAAA ACATCGTCAA ACAGACGCTG 
ATGGAGAATG GCATTACGCG CCTCTGCGTG TTCTTCGGCA GGCAGAAAGC CAATGACGTC 
CTTCTATCCC ACATGATCAC CTTCCTGAAT GACAAGAACG ACTGGCATCT CCGCGGAGCT 
TTCTTTGACA GCATTGTAGG TGTAGCAGCA TATGTGGGCT GGCAGAGCTC CTCCATCCTG 
AAGCCTCTTC TCCAGCAAGG CCTGAGTGAC GTTGAAGAGT TTGTCATTTA TAAAGCTCTC 
AATGCTCTGA CCTGCATGTG TCAGCTGGGG CTGTTGCAGA AGCCTCACAT CTATGAGTTT 
GTCAGCGATA TTGCTCCATT CTTGTGCCAC CCCAATCTGT GGATCCGCTA TGGAGCTGTG 
GGTTTCATCA CTGTCGTCGC TCAGCACCTA AACATCGCTG ATGTTTACTG CAAACTTATG 
CCACATCTTA ACCCTTTCAT CACACAACCC ATTATACAGA TTGATAAGGA GATTGTGTTG 
CTCAGTGTGC TGAAAGAGCC TGTAAGTCGC TCCATCTTTG ACTATGCACT GCGTTCCAAA 
GACATCGGAA GCCTTTTCAG ACACCTGTTA CTCCGGCAAA AGAAGCGCAA CGGCTCCATC 
CCAGAATGCC CCATTCCTGA GGATCCTGCA ATTGCCCAGC TTCTTAAAAA ACTTCTCTCA 
CAGGGCATGA CAGAGGCTGA AGAGGATAAG CTCTTGGCTC TGAAAGATTT CATGCTCAAG 
TCTAACAAGG CCAAAGCCAA CATCATAGAC CAGAGCCACC TGAGTGAAGG AGCCCACAGT 
GGAGTCATTG ATCTCGGGAC ACTGGGCATC ACTGGCCGAC AGGTGGACCT GGTCAAGCCC 
AAGCAGGAGA TTGAAGATAA GAGAGCTCGC AAACATACGA AACAGGACTC CAACTTGAAC 
GAAGAGTGGA AGAGCATGTT TGGTTCTCTG GATCCTCCCA GCTCGGCATC CACGCCCTCG 
ACGATTCCAG ACAGTGGAGC CGCGCAGCCC CGGAAAACCA CAGCGCTTCC TACTATTCAA 
GTGGTTCAGT CGATAACTGG AGCGTCCACA TATCAGCGGC GCATCACCAC ATGTAAAGCA 
GAGCTGCAGC AGCTGGTGCA GCAGAAGAGA GAGCAGTGCA ATGCAGAGCG CATGGCCAAA 
CAGATGATGG AGAGCGCAGA GTGGGAAAGC AGACCTCCAC TGCCAGGGTG GCATCCAAAA 
GGTCTTCTAG TGGCCCATCT ACATGAGCAC AAATCTGCTG TCAACCGAAT CCGAGTCTCA 
GATGAGCACT CCATCTTTGC TACCTGCTCC AATGATGGAA CTGTCAAAAT CTGGGACAGC 
CAAAAAATGG AGGGCAAGAC GACTACCACG AGGTCTGTGC TGACATATTC TCGGATTGGA 
GGTCATGTGA AAACATTGAC ATTTTGCCAG GGATCGCATT ACCTTGCAGT TGCATCAGAT 
AATGGTTCCA TCCAACTCCT TGCAGTCGAG GCAAACAAGC CTCCCAAATC ACCCAAAGTC 
CAGCCCTGTC AGACAAGGTT TTTGGATCCA AAGGAGGAGG GCTGTGTGGT GGACGTACAT 
CATTTTAACT CTGGGGCGCA GACGGTTTTG GCCTACGCTA CTGTAAATGG CTTTTTGGTC 
GGTTGGGACC TGCGAAGCAA CAGCAACGCA TGGACTCTTC GCCATGATCT GCGTCTGGGC 
CTCATCACTT CCTTTGCTGT TGATATGCAC CAGTGTTGGC TGTGTGTAGG CACAAGTAAT 
GGCACAATGG CATGCTGGGA TATGCGGTTT CAGCTACCAA TTTCCAGTCA CTCCCATCCA 
GCCCGAGCTC GGATCAGACG CCTTCTGATG CATCCACTCT ATCAGTCATC TGTCATTGCA 
GCCGTTCAGG GTAACAATGA GGTCTCCATG TGGGATATGG AGACCGGAGA CAGGAAGTTC 
ACCCTGTGGG CGAGCTCTGC GCCTCCGCTT TCGGAGATGC AGCCCTCTCC ACACAGTGTA 
CATGGCATAT ACTGCAGCCC TGCGGATGGA AACCCTCTCC TCCTCACAGC CGGATCTGAC 
ATGAGAATCA GGTTTTGGGA TTTGGCTTAC CCTGAGAGAT CATACATCGT CGCAGGCGGT 
GCTAACGACT CCCTCCACTG CCCTTCTGTT TTCTACAACC GCAAGATCAT CGAAGGAACA 
GAAGTGGTTC AGGAGATTCA CAGTAAACAG AAGAGCGGCT CCACCGAGGA TACTCCTCGC 
CGAGGCCCAG AATCCCTTCC AGTGGGCCAC CATGACATCA TAACAGACAT CGCCACTTTC 
CAGACCACAC AGGGCTTTAT AGTCACTTCC TCTAGAGATG GCATTGTCAA AGTCTGGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3R4 ( XM_005158299.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005158356.1 Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X2, mRNA.

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CloneID	ODa21701
Clone ID Related Accession (Same CDS sequence)	XM_005158299.4 , XM_005158299.3
Accession Version	XM_005158299.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	phosphoinositide 3-kinase regulatory subunit 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158299.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAACC AACTGGCTGG AATTGCTCCA TCGCAGATCC TGTCAGTGGA CAGTTACTTC 
TCTGACATCC ATGACTACGA ATATGACAAA AGTCTGGGCA GTACACGATT TTTCAAAGTG 
GCCCGGGCGA AACACAGGGA AGGATTGGTC GTTGTTAAGG TCTTTGCTAT CCAAGACCCT 
TCTCTACCCC TGACCAGCTA CAAACAAGAG GTGGAGGAGC TGAAAATCCG ACTGCACTCC 
TGTCAGAACT GCCTGCCTTT CCAAAAAGCC ACGCTTACTG AAAAAGCAGC CATTCTGTTT 
CGCCAGTATG TTCGGGATAA CTTGTATGAC CGTATTAGTA CGCGGCCTTT CCTCAACAAC 
GTGGAAAAGA AGTGGATCGC CTTCCAGCTC CTCAATGCTG TGGACCAGGC CCATAAATCG 
GGAGTGCGTC ACGGAGACAT TAAAACAGAG AACGTCATGG TGACAAGCTG GAACTGGGTG 
CTTCTAACAG ACTTTGCTAG CTTTAAGCCA ACATACTTGC CTGAAGACAA TCCCGCAGAT 
TTTAATTACT TCTTCGACAC GTCCAGGAGG AGAACATGTT ACATTGCACC TGAGAGATTT 
GTGGATGGCA GCATGTTTGC TACAGAAAGT GACCAGACCA CTCCTCTCGT AGATCTTTCT 
AGTAATAGTC AGAGGACCAG AGGGGAGCTC AAGCAGCCCA TGGACATCTT CTCAGCTGGT 
TGTGTGATTG CGGAATTGTT CACAGAGGGC GTCCCGCTCT TTGACCTCTC ACAACTGCTG 
GCTTATCGTA AAGGGCATTT CCAAACCGAA CAAGTGCTGA TGAAAATTGA AGATCACAGT 
ATTCGAGAAC TGGTGGCTCA AATGGTGCAG AGAGAGCCAG AAAAGCGACT GACAGCTGAA 
GAATACCTTA AGCAGCAGCG AGGAAAAGCT TTCCCAGAAA TCTTCTACAC CTTCCTTCAA 
CCGTACATGG CCCAGTTTGC CAAGGAGACC TTCCAGTCTG CTGACGAAAG AGTCTTGGTG 
ATCCGTAAAA ACCTGGAGAA CATCCTCAAC AACCTGTGTA GCGGCGGGCA GACGGCGAAG 
GCTGAAAAAC AGGAGAAAGC ATCCCAAGAG TTTAGCTCAT CTAAAGAACA AGGCCTGGTG 
GTGCTGGTGT CAGTGATCAC TTCATGTCTA CAGACGCTTC GATTTTGTGA CTCCAAGCTG 
GCGGCGCTGG AGCTGATCCT TCATCTGGCA GGCCGGCTCA ATGTGGAGAT TCTGCTGGAC 
AGGATTACGC CGTATTTGCT GCACTTCTGC AATGACTCTA TGCCGCGTGT CAGAGCTGAG 
GCCGTTCGCA CGCTGACTAA GGTGCTGGCG CTGGTCAAGG AGGTTCCCCG CAATGATGTC 
AACATCTACC CGGAGTACAT TTTGCCTGGC ATTGCCCACT TGGCCCAAGA TGAAGCCACG 
ATTGTCAGAC TTGCTTATGC AGAAAACATA GCCCATCTAG CCGAGACAGC ACTGCGTTTT 
CTGGAGCTGG TGCAGGAGAA TAATTTGAGC TCTGAACACG ATCTGAATGG AGAGGACACA 
GAAGAAACTC TTCATCCCAA TGAAAACTAT GATTCAGAGT TGCAGGCCTT GCATGAGATG 
GTTCAGCAGA AGGTGGTGAC TTTGCTGAGC GATCCTGAAA ACATCGTCAA ACAGACGCTG 
ATGGAGAATG GCATTACGCG CCTCTGCGTG TTCTTCGGCA GGCAGAAAGC CAATGACGTC 
CTTCTATCCC ACATGATCAC CTTCCTGAAT GACAAGAACG ACTGGCATCT CCGCGGAGCT 
TTCTTTGACA GCATTGTAGG TGTAGCAGCA TATGTGGGCT GGCAGAGCTC CTCCATCCTG 
AAGCCTCTTC TCCAGCAAGG CCTGAGTGAC GTTGAAGAGT TTGTCATTTA TAAAGCTCTC 
AATGCTCTGA CCTGCATGTG TCAGCTGGGG CTGTTGCAGA AGCCTCACAT CTATGAGTTT 
GTCAGCGATA TTGCTCCATT CTTGTGCCAC CCCAATCTGT GGATCCGCTA TGGAGCTGTG 
GGTTTCATCA CTGTCGTCGC TCAGCACCTA AACATCGCTG ATGTTTACTG CAAACTTATG 
CCACATCTTA ACCCTTTCAT CACACAACCC ATTATACAGA TTGATAAGGA GATTGTGTTG 
CTCAGTGTGC TGAAAGAGCC TGTAAGTCGC TCCATCTTTG ACTATGCACT GCGTTCCAAA 
GACATCGGAA GCCTTTTCAG ACACCTGTTA CTCCGGCAAA AGAAGCGCAA CGGCTCCATC 
CCAGAATGCC CCATTCCTGA GGATCCTGCA ATTGCCCAGC TTCTTAAAAA ACTTCTCTCA 
CAGGGCATGA CAGAGGCTGA AGAGGATAAG CTCTTGGCTC TGAAAGATTT CATGCTCAAG 
TCTAACAAGG CCAAAGCCAA CATCATAGAC CAGAGCCACC TGAGTGAAGG AGCCCACAGT 
GGAGTCATTG ATCTCGGGAC ACTGGGCATC ACTGGCCGAC AGGTGGACCT GGTCAAGCCC 
AAGCAGGAGA TTGAAGATAA GAGAGCTCGC AAACATACGA AACAGGACTC CAACTTGAAC 
GAAGAGTGGA AGAGCATGTT TGGTTCTCTG GATCCTCCCA GCTCGGCATC CACGCCCTCG 
ACGATTCCAG ACAGTGGAGC CGCGCAGCCC CGGAAAACCA CAGCGCTTCC TACTATTCAA 
GTGGTTCAGT CGATAACTGG AGCGTCCACA TATCAGCGGC GCATCACCAC ATGTAAAGCA 
GAGCTGCAGC AGCTGGTGCA GCAGAAGAGA GAGCAGTGCA ATGCAGAGCG CATGGCCAAA 
CAGATGATGG AGAGCGCAGA GTGGGAAAGC AGACCTCCAC TGCCAGGGTG GCATCCAAAA 
GGTCTTCTAG TGGCCCATCT ACATGAGCAC AAATCTGCTG TCAACCGAAT CCGAGTCTCA 
GATGAGCACT CCATCTTTGC TACCTGCTCC AATGATGGAA CTGTCAAAAT CTGGGACAGC 
CAAAAAATGG AGGGCAAGAC GACTACCACG AGGTCTGTGC TGACATATTC TCGGATTGGA 
GGTCATGTGA AAACATTGAC ATTTTGCCAG GGATCGCATT ACCTTGCAGT TGCATCAGAT 
AATGGTTCCA TCCAACTCCT TGCAGTCGAG GCAAACAAGC CTCCCAAATC ACCCAAAGTC 
CAGCCCTGTC AGACAAGGTT TTTGGATCCA AAGGAGGAGG GCTGTGTGGT GGACGTACAT 
CATTTTAACT CTGGGGCGCA GACGGTTTTG GCCTACGCTA CTGTAAATGG CTTTTTGGTC 
GGTTGGGACC TGCGAAGCAA CAGCAACGCA TGGACTCTTC GCCATGATCT GCGTCTGGGC 
CTCATCACTT CCTTTGCTGT TGATATGCAC CAGTGTTGGC TGTGTGTAGG CACAAGTAAT 
GGCACAATGG CATGCTGGGA TATGCGGTTT CAGCTACCAA TTTCCAGTCA CTCCCATCCA 
GCCCGAGCTC GGATCAGACG CCTTCTGATG CATCCACTCT ATCAGTCATC TGTCATTGCA 
GCCGTTCAGG GTAACAATGA GGTCTCCATG TGGGATATGG AGACCGGAGA CAGGAAGTTC 
ACCCTGTGGG CGAGCTCTGC GCCTCCGCTT TCGGAGATGC AGCCCTCTCC ACACAGTGTA 
CATGGCATAT ACTGCAGCCC TGCGGATGGA AACCCTCTCC TCCTCACAGC CGGATCTGAC 
ATGAGAATCA GGTTTTGGGA TTTGGCTTAC CCTGAGAGAT CATACATCGT CGCAGGCGGT 
GCTAACGACT CCCTCCACTG CCCTTCTGTT TTCTACAACC GCAAGATCAT CGAAGGAACA 
GAAGTGGTTC AGGAGATTCA CAGTAAACAG AAGAGCGGCT CCACCGAGGA TACTCCTCGC 
CGAGGCCCAG AATCCCTTCC AGTGGGCCAC CATGACATCA TAACAGACAT CGCCACTTTC 
CAGACCACAC AGGGCTTTAT AGTCACTTCC TCTAGAGATG GCATTGTCAA AGTCTGGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158356.1
CDS	313..4335
Translation	MGNQLAGIAPSQILSVDSYFSDIHDYEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPSLPLTSYKQEVEELKIRLHSCQNCLPFQKATLTEKAAILFRQYVRDNLYDRISTRPFLNNVEKKWIAFQLLNAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGSMFATESDQTTPLVDLSSNSQRTRGELKQPMDIFSAGCVIAELFTEGVPLFDLSQLLAYRKGHFQTEQVLMKIEDHSIRELVAQMVQREPEKRLTAEEYLKQQRGKAFPEIFYTFLQPYMAQFAKETFQSADERVLVIRKNLENILNNLCSGGQTAKAEKQEKASQEFSSSKEQGLVVLVSVITSCLQTLRFCDSKLAALELILHLAGRLNVEILLDRITPYLLHFCNDSMPRVRAEAVRTLTKVLALVKEVPRNDVNIYPEYILPGIAHLAQDEATIVRLAYAENIAHLAETALRFLELVQENNLSSEHDLNGEDTEETLHPNENYDSELQALHEMVQQKVVTLLSDPENIVKQTLMENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDVEEFVIYKALNALTCMCQLGLLQKPHIYEFVSDIAPFLCHPNLWIRYGAVGFITVVAQHLNIADVYCKLMPHLNPFITQPIIQIDKEIVLLSVLKEPVSRSIFDYALRSKDIGSLFRHLLLRQKKRNGSIPECPIPEDPAIAQLLKKLLSQGMTEAEEDKLLALKDFMLKSNKAKANIIDQSHLSEGAHSGVIDLGTLGITGRQVDLVKPKQEIEDKRARKHTKQDSNLNEEWKSMFGSLDPPSSASTPSTIPDSGAAQPRKTTALPTIQVVQSITGASTYQRRITTCKAELQQLVQQKREQCNAERMAKQMMESAEWESRPPLPGWHPKGLLVAHLHEHKSAVNRIRVSDEHSIFATCSNDGTVKIWDSQKMEGKTTTTRSVLTYSRIGGHVKTLTFCQGSHYLAVASDNGSIQLLAVEANKPPKSPKVQPCQTRFLDPKEEGCVVDVHHFNSGAQTVLAYATVNGFLVGWDLRSNSNAWTLRHDLRLGLITSFAVDMHQCWLCVGTSNGTMACWDMRFQLPISSHSHPARARIRRLLMHPLYQSSVIAAVQGNNEVSMWDMETGDRKFTLWASSAPPLSEMQPSPHSVHGIYCSPADGNPLLLTAGSDMRIRFWDLAYPERSYIVAGGANDSLHCPSVFYNRKIIEGTEVVQEIHSKQKSGSTEDTPRRGPESLPVGHHDIITDIATFQTTQGFIVTSSRDGIVKVWK

Target ORF information:

RefSeq Version	XM_005158299.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphoinositide-3-kinase, regulatory subunit 4 (pik3r4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158299.3

ORF Insert Sequence:

ATGGGGAACC AACTGGCTGG AATTGCTCCA TCGCAGATCC TGTCAGTGGA CAGTTACTTC 
TCTGACATCC ATGACTACGA ATATGACAAA AGTCTGGGCA GTACACGATT TTTCAAAGTG 
GCCCGGGCGA AACACAGGGA AGGATTGGTC GTTGTTAAGG TCTTTGCTAT CCAAGACCCT 
TCTCTACCCC TGACCAGCTA CAAACAAGAG GTGGAGGAGC TGAAAATCCG ACTGCACTCC 
TGTCAGAACT GCCTGCCTTT CCAAAAAGCC ACGCTTACTG AAAAAGCAGC CATTCTGTTT 
CGCCAGTATG TTCGGGATAA CTTGTATGAC CGTATTAGTA CGCGGCCTTT CCTCAACAAC 
GTGGAAAAGA AGTGGATCGC CTTCCAGCTC CTCAATGCTG TGGACCAGGC CCATAAATCG 
GGAGTGCGTC ACGGAGACAT TAAAACAGAG AACGTCATGG TGACAAGCTG GAACTGGGTG 
CTTCTAACAG ACTTTGCTAG CTTTAAGCCA ACATACTTGC CTGAAGACAA TCCCGCAGAT 
TTTAATTACT TCTTCGACAC GTCCAGGAGG AGAACATGTT ACATTGCACC TGAGAGATTT 
GTGGATGGCA GCATGTTTGC TACAGAAAGT GACCAGACCA CTCCTCTCGT AGATCTTTCT 
AGTAATAGTC AGAGGACCAG AGGGGAGCTC AAGCAGCCCA TGGACATCTT CTCAGCTGGT 
TGTGTGATTG CGGAATTGTT CACAGAGGGC GTCCCGCTCT TTGACCTCTC ACAACTGCTG 
GCTTATCGTA AAGGGCATTT CCAAACCGAA CAAGTGCTGA TGAAAATTGA AGATCACAGT 
ATTCGAGAAC TGGTGGCTCA AATGGTGCAG AGAGAGCCAG AAAAGCGACT GACAGCTGAA 
GAATACCTTA AGCAGCAGCG AGGAAAAGCT TTCCCAGAAA TCTTCTACAC CTTCCTTCAA 
CCGTACATGG CCCAGTTTGC CAAGGAGACC TTCCAGTCTG CTGACGAAAG AGTCTTGGTG 
ATCCGTAAAA ACCTGGAGAA CATCCTCAAC AACCTGTGTA GCGGCGGGCA GACGGCGAAG 
GCTGAAAAAC AGGAGAAAGC ATCCCAAGAG TTTAGCTCAT CTAAAGAACA AGGCCTGGTG 
GTGCTGGTGT CAGTGATCAC TTCATGTCTA CAGACGCTTC GATTTTGTGA CTCCAAGCTG 
GCGGCGCTGG AGCTGATCCT TCATCTGGCA GGCCGGCTCA ATGTGGAGAT TCTGCTGGAC 
AGGATTACGC CGTATTTGCT GCACTTCTGC AATGACTCTA TGCCGCGTGT CAGAGCTGAG 
GCCGTTCGCA CGCTGACTAA GGTGCTGGCG CTGGTCAAGG AGGTTCCCCG CAATGATGTC 
AACATCTACC CGGAGTACAT TTTGCCTGGC ATTGCCCACT TGGCCCAAGA TGAAGCCACG 
ATTGTCAGAC TTGCTTATGC AGAAAACATA GCCCATCTAG CCGAGACAGC ACTGCGTTTT 
CTGGAGCTGG TGCAGGAGAA TAATTTGAGC TCTGAACACG ATCTGAATGG AGAGGACACA 
GAAGAAACTC TTCATCCCAA TGAAAACTAT GATTCAGAGT TGCAGGCCTT GCATGAGATG 
GTTCAGCAGA AGGTGGTGAC TTTGCTGAGC GATCCTGAAA ACATCGTCAA ACAGACGCTG 
ATGGAGAATG GCATTACGCG CCTCTGCGTG TTCTTCGGCA GGCAGAAAGC CAATGACGTC 
CTTCTATCCC ACATGATCAC CTTCCTGAAT GACAAGAACG ACTGGCATCT CCGCGGAGCT 
TTCTTTGACA GCATTGTAGG TGTAGCAGCA TATGTGGGCT GGCAGAGCTC CTCCATCCTG 
AAGCCTCTTC TCCAGCAAGG CCTGAGTGAC GTTGAAGAGT TTGTCATTTA TAAAGCTCTC 
AATGCTCTGA CCTGCATGTG TCAGCTGGGG CTGTTGCAGA AGCCTCACAT CTATGAGTTT 
GTCAGCGATA TTGCTCCATT CTTGTGCCAC CCCAATCTGT GGATCCGCTA TGGAGCTGTG 
GGTTTCATCA CTGTCGTCGC TCAGCACCTA AACATCGCTG ATGTTTACTG CAAACTTATG 
CCACATCTTA ACCCTTTCAT CACACAACCC ATTATACAGA TTGATAAGGA GATTGTGTTG 
CTCAGTGTGC TGAAAGAGCC TGTAAGTCGC TCCATCTTTG ACTATGCACT GCGTTCCAAA 
GACATCGGAA GCCTTTTCAG ACACCTGTTA CTCCGGCAAA AGAAGCGCAA CGGCTCCATC 
CCAGAATGCC CCATTCCTGA GGATCCTGCA ATTGCCCAGC TTCTTAAAAA ACTTCTCTCA 
CAGGGCATGA CAGAGGCTGA AGAGGATAAG CTCTTGGCTC TGAAAGATTT CATGCTCAAG 
TCTAACAAGG CCAAAGCCAA CATCATAGAC CAGAGCCACC TGAGTGAAGG AGCCCACAGT 
GGAGTCATTG ATCTCGGGAC ACTGGGCATC ACTGGCCGAC AGGTGGACCT GGTCAAGCCC 
AAGCAGGAGA TTGAAGATAA GAGAGCTCGC AAACATACGA AACAGGACTC CAACTTGAAC 
GAAGAGTGGA AGAGCATGTT TGGTTCTCTG GATCCTCCCA GCTCGGCATC CACGCCCTCG 
ACGATTCCAG ACAGTGGAGC CGCGCAGCCC CGGAAAACCA CAGCGCTTCC TACTATTCAA 
GTGGTTCAGT CGATAACTGG AGCGTCCACA TATCAGCGGC GCATCACCAC ATGTAAAGCA 
GAGCTGCAGC AGCTGGTGCA GCAGAAGAGA GAGCAGTGCA ATGCAGAGCG CATGGCCAAA 
CAGATGATGG AGAGCGCAGA GTGGGAAAGC AGACCTCCAC TGCCAGGGTG GCATCCAAAA 
GGTCTTCTAG TGGCCCATCT ACATGAGCAC AAATCTGCTG TCAACCGAAT CCGAGTCTCA 
GATGAGCACT CCATCTTTGC TACCTGCTCC AATGATGGAA CTGTCAAAAT CTGGGACAGC 
CAAAAAATGG AGGGCAAGAC GACTACCACG AGGTCTGTGC TGACATATTC TCGGATTGGA 
GGTCATGTGA AAACATTGAC ATTTTGCCAG GGATCGCATT ACCTTGCAGT TGCATCAGAT 
AATGGTTCCA TCCAACTCCT TGCAGTCGAG GCAAACAAGC CTCCCAAATC ACCCAAAGTC 
CAGCCCTGTC AGACAAGGTT TTTGGATCCA AAGGAGGAGG GCTGTGTGGT GGACGTACAT 
CATTTTAACT CTGGGGCGCA GACGGTTTTG GCCTACGCTA CTGTAAATGG CTTTTTGGTC 
GGTTGGGACC TGCGAAGCAA CAGCAACGCA TGGACTCTTC GCCATGATCT GCGTCTGGGC 
CTCATCACTT CCTTTGCTGT TGATATGCAC CAGTGTTGGC TGTGTGTAGG CACAAGTAAT 
GGCACAATGG CATGCTGGGA TATGCGGTTT CAGCTACCAA TTTCCAGTCA CTCCCATCCA 
GCCCGAGCTC GGATCAGACG CCTTCTGATG CATCCACTCT ATCAGTCATC TGTCATTGCA 
GCCGTTCAGG GTAACAATGA GGTCTCCATG TGGGATATGG AGACCGGAGA CAGGAAGTTC 
ACCCTGTGGG CGAGCTCTGC GCCTCCGCTT TCGGAGATGC AGCCCTCTCC ACACAGTGTA 
CATGGCATAT ACTGCAGCCC TGCGGATGGA AACCCTCTCC TCCTCACAGC CGGATCTGAC 
ATGAGAATCA GGTTTTGGGA TTTGGCTTAC CCTGAGAGAT CATACATCGT CGCAGGCGGT 
GCTAACGACT CCCTCCACTG CCCTTCTGTT TTCTACAACC GCAAGATCAT CGAAGGAACA 
GAAGTGGTTC AGGAGATTCA CAGTAAACAG AAGAGCGGCT CCACCGAGGA TACTCCTCGC 
CGAGGCCCAG AATCCCTTCC AGTGGGCCAC CATGACATCA TAACAGACAT CGCCACTTTC 
CAGACCACAC AGGGCTTTAT AGTCACTTCC TCTAGAGATG GCATTGTCAA AGTCTGGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PIK3R4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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