ITGB5 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ITGB5
Entrez Gene ID	563556
Full Name	integrin, beta 5
Synonyms	wu:fi30h12
General protein information	Preferred Names integrin, beta 5 Names integrin beta-5
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001082836.1	NP_001076305.1	integrin beta-5
XM_009304631.1	XP_009302906.1	integrin beta-5 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Itgb5	NP_001139356.1
Canis lupus familiaris (dog)	ITGB5	XP_851245.2
Homo sapiens (human)	ITGB5	NP_002204.2
Bos taurus (cattle)	ITGB5	NP_777104.1
Rattus norvegicus (Norway rat)	Itgb5	NP_671480.2
Danio rerio (zebrafish)	itgb5	NP_001076305.1
Xenopus tropicalis (tropical clawed frog)	LOC100216279	NP_001135704.1
Pan troglodytes (chimpanzee)	ITGB5	XP_516706.4
Macaca mulatta (Rhesus monkey)	ITGB5	XP_001113909.1
Gallus gallus (chicken)	ITGB5	NP_989814.1

Gene Ontology
Bibliography

Related articles in PubMed

Activation of Hepatic Stellate Cells During Liver Carcinogenesis Requires Fibrinogen/Integrin αvβ5 in Zebrafish.
Yan C, Yang Q, Gong Z
Neoplasia (New York, N.Y.)20(5)533-542(2018 05)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Integrin alphaV is necessary for gastrulation movements that regulate vertebrate body asymmetry.
Ablooglu AJ, Tkachenko E, Kang J, Shattil SJ
Development (Cambridge, England)137(20)3449-58(2010 Oct)

MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis.
Nicoli S, Standley C, Walker P, Hurlstone A, Fogarty KE, Lawson ND
Nature464(7292)1196-200(2010 Apr)

Nlcam modulates midline convergence during anterior neural plate morphogenesis.
Brown KE, Keller PJ, Ramialison M, Rembold M, Stelzer EH, Loosli F, Wittbrodt J
Developmental biology339(1)14-25(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ITGB5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITGB5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01637	NM_001082836.1 Latest version!	Danio rerio integrin, beta 5 (itgb5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa44109	XM_009304631.1 Latest version!	Danio rerio integrin, beta 5 (itgb5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITGB5 ( NM_001082836.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001076305.1 Danio rerio integrin, beta 5 (itgb5), mRNA.

Price & Availability↑

CloneID	ODa01637
Clone ID Related Accession (Same CDS sequence)	NM_001082836.1
Accession Version	NM_001082836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	integrin beta-5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL974314.21. On Mar 15, 2007 this sequence version replaced XM_686922.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGTGCCGCT GTGATTTGGG AAGGATGGAC AAAGAGAAGA CCTCAGTCAC CCGCTGTGGA 
AGGATGTGGA AACTTTGCTC ATCTACACGC CACCCGGCTC TTCTAGTTTG TATAGTTTTG 
GAAATATTTA TTCACAGCGT CAAAGGTCTT AATGTTTGTA TGAGCGGCAG CGCCACGTCT 
TGTGAAGAGT GTCTCCTCAT TCACCCGAGC TGCGCCTGGT GCGCGCAAGA GGACTTTGGA 
CAGGCCAGAA CACTTACCTC AAGATGTGAC TTTAGCCAGA ACTTACAGAA GAGGGGATGC 
GATGCACCAT TTATAGAGAA CCCGAGGAGT GGCAGCTCTG TGCTCCAGAG CAAACCCTTG 
AGCTACAAAG GGTCAGGAGT GACCCAGTAT GATGTCATCC AGATATACCC ACAGAAGATC 
TCTCTCAGCT TAAGGCCTGG TGACCAGGCG TCTTTTGAAG TCCAAGTGCG CCCTGTGGAA 
GACTATCCTG TTGATTTGTA CTACCTCATG GACCTCTCGC TATCCATGAA GGACGATCTG 
GACACGATCC GCAACCTGGG CACCAAGCTG GCTGAAGAAA TGAGGAAGCT CACGAGTAAC 
TTTCGTCTTG GGTTTGGTTC CTTTGTGGAC AAGAACATCT CACCCTTCTC CTACACTGCA 
TCCAAGTACC AGGACAACCC ATGCAATGGA TATAAATTGT TCCCAAACTG CGTCCCAACA 
TTTGGCTTTC GTCACCTCTT GTCTCTTACG GACAAGGTTG ATCGTTTCAA TGAAGAAGTG 
CAGAAGCAGA TGGTTTCGAG AAACAGGGAC GCGCCAGAGG GAGGGTTTGA TGCCATTCTT 
CAGGCTGCAG TTTGCAGGGA GAAGATTGGC TGGAGGAAGG AGGCCTATCA TCTGCTGGTG 
TTTGCCACTG ATGACGTCCC TCACTTGGCT TTGGATGGTA AACTCGGTGG ACTGGTGCAT 
CCTCATGACG GCCAGTGTCA CCTGAATGAC AAGAATGAGT ACAGTGCTTC AACCAAGATG 
GACTACCCTT CTCTAGCACT GCTTGGAGAA AAACTGGCTG AAAACAATAT TTTCCTGATC 
TTCGCCGTTA TAAAAAGACA CTACGTTCTT TACAAGAACC TCACTGCGTT GATCCCTGGG 
ACTACAGTGG AAATTTTGGA CCAGGACTCC AAAAACATCA TTCAACTAAT TGTGAACGCC 
TACAATAACA TAAGGTCAAA AGTTGAGCTG AGCGTGTGGG ATCATCCTGA AGATCTCAGT 
CTGGCCTTCT CCGCTACCTG TCAAGATGGA CAGCCCCTGA CCGGTCTCCG CAAGTGCGCC 
GACCTCAAAA TTGGTGACAC TGTCTCCTTC AATGTAACCG TAGACGCTCG AGGATGTCCT 
CCGAAAGGCA CCAGGAAGAG TTTCACCATC AGGCCTGTTG GTTTTAAAGA CCGTCTAGAG 
GTTTCTGTGG ATTACCGCTG TGATTGCCGC TGCACGTATC ACACAAAGCC GAACAGCAGC 
CGCTGCAACT CTGCTGGCAC TTACAACTGT GGGTTGTGCC GCTGTGAGCC TGGGTATCTG 
GGAGCCCGCT GCGAGTGCCA GGAGGGTGAG GTGGACCACC AGCACCTCAG TGCCTGTCGA 
GAAGCAGAGG GCAAGCAGGT GTGCAGTGGT CGTGGAGAAT GCAGCTGTAA CCAGTGCCTG 
TGCTATGAGT CCGAGTTTGG CAAGATCTAC GGCACCTTCT GCGAATGTGA TGACTTCTCC 
TGTGCCAGAC ACAAGGGCAT ACTCTGCTCA GGTCATGGAG AATGCCACTG TGGTGAGTGT 
AAATGCCATG CCGGTTATAT TGGAGACAAC TGTAATTGCT CGACGGAAAC GGCTTCCTGT 
GTGTCGGATG ATGGCAAGAT CTGTAGCGGC CGGGGCAGCT GTGTGTGCGG ACGCTGCCAG 
TGCACTGAAC CAGGGGCATT TGGGGACACA TGTGAAAAAT GTCCCACCTG TCCAGATGCA 
TGTGGAACAA AGAGGGAATG CATCGAGTGT CGTCTTTTTA ACACAGGAAG ACTGGCTGAT 
AATCAGACCT GTCAGCGCAT GTGCAAGGAC GAAATCACCA CAGTGGAAAC CCTCAAAACT 
GACGATCCCA ACGCTGTTCA GTGTGTGTAT AAGACTGAGA ATGACTGCGT GATGAAGTTT 
ACATACTCTG AACATGCCAA TGGACAATCA GTCCTCACCG CTCTTAAAGA GCCAGAATGT 
GCCGTCGCAC CAAATGCTAT GACCGTGCTG CTGGCTGTGG TTGGCAGTAT CTTGTTGATG 
GGCATCGTGC TACTGGCTCT CTGGAAACTG GTCATCACTG TTCATGACCG CAGGGAGTTT 
GCACGCTTTC AAAGTGCACG TTCACGAGCG CGATATGAGA TGGCATCTAA TCCAGTTTAT 
AAGCGGTCCG TACCAATGGA GACGGATTTC GATATGCATG GTATCAAGTC ACTGAACGGA 
GGAGTCCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076305.1
CDS	289..2760
Translation	MCRCDLGRMDKEKTSVTRCGRMWKLCSSTRHPALLVCIVLEIFIHSVKGLNVCMSGSATSCEECLLIHPSCAWCAQEDFGQARTLTSRCDFSQNLQKRGCDAPFIENPRSGSSVLQSKPLSYKGSGVTQYDVIQIYPQKISLSLRPGDQASFEVQVRPVEDYPVDLYYLMDLSLSMKDDLDTIRNLGTKLAEEMRKLTSNFRLGFGSFVDKNISPFSYTASKYQDNPCNGYKLFPNCVPTFGFRHLLSLTDKVDRFNEEVQKQMVSRNRDAPEGGFDAILQAAVCREKIGWRKEAYHLLVFATDDVPHLALDGKLGGLVHPHDGQCHLNDKNEYSASTKMDYPSLALLGEKLAENNIFLIFAVIKRHYVLYKNLTALIPGTTVEILDQDSKNIIQLIVNAYNNIRSKVELSVWDHPEDLSLAFSATCQDGQPLTGLRKCADLKIGDTVSFNVTVDARGCPPKGTRKSFTIRPVGFKDRLEVSVDYRCDCRCTYHTKPNSSRCNSAGTYNCGLCRCEPGYLGARCECQEGEVDHQHLSACREAEGKQVCSGRGECSCNQCLCYESEFGKIYGTFCECDDFSCARHKGILCSGHGECHCGECKCHAGYIGDNCNCSTETASCVSDDGKICSGRGSCVCGRCQCTEPGAFGDTCEKCPTCPDACGTKRECIECRLFNTGRLADNQTCQRMCKDEITTVETLKTDDPNAVQCVYKTENDCVMKFTYSEHANGQSVLTALKEPECAVAPNAMTVLLAVVGSILLMGIVLLALWKLVITVHDRREFARFQSARSRARYEMASNPVYKRSVPMETDFDMHGIKSLNGGVH

Target ORF information:

RefSeq Version	NM_001082836.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio integrin, beta 5 (itgb5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082836.1

ORF Insert Sequence:

ATGTGCCGCT GTGATTTGGG AAGGATGGAC AAAGAGAAGA CCTCAGTCAC CCGCTGTGGA 
AGGATGTGGA AACTTTGCTC ATCTACACGC CACCCGGCTC TTCTAGTTTG TATAGTTTTG 
GAAATATTTA TTCACAGCGT CAAAGGTCTT AATGTTTGTA TGAGCGGCAG CGCCACGTCT 
TGTGAAGAGT GTCTCCTCAT TCACCCGAGC TGCGCCTGGT GCGCGCAAGA GGACTTTGGA 
CAGGCCAGAA CACTTACCTC AAGATGTGAC TTTAGCCAGA ACTTACAGAA GAGGGGATGC 
GATGCACCAT TTATAGAGAA CCCGAGGAGT GGCAGCTCTG TGCTCCAGAG CAAACCCTTG 
AGCTACAAAG GGTCAGGAGT GACCCAGTAT GATGTCATCC AGATATACCC ACAGAAGATC 
TCTCTCAGCT TAAGGCCTGG TGACCAGGCG TCTTTTGAAG TCCAAGTGCG CCCTGTGGAA 
GACTATCCTG TTGATTTGTA CTACCTCATG GACCTCTCGC TATCCATGAA GGACGATCTG 
GACACGATCC GCAACCTGGG CACCAAGCTG GCTGAAGAAA TGAGGAAGCT CACGAGTAAC 
TTTCGTCTTG GGTTTGGTTC CTTTGTGGAC AAGAACATCT CACCCTTCTC CTACACTGCA 
TCCAAGTACC AGGACAACCC ATGCAATGGA TATAAATTGT TCCCAAACTG CGTCCCAACA 
TTTGGCTTTC GTCACCTCTT GTCTCTTACG GACAAGGTTG ATCGTTTCAA TGAAGAAGTG 
CAGAAGCAGA TGGTTTCGAG AAACAGGGAC GCGCCAGAGG GAGGGTTTGA TGCCATTCTT 
CAGGCTGCAG TTTGCAGGGA GAAGATTGGC TGGAGGAAGG AGGCCTATCA TCTGCTGGTG 
TTTGCCACTG ATGACGTCCC TCACTTGGCT TTGGATGGTA AACTCGGTGG ACTGGTGCAT 
CCTCATGACG GCCAGTGTCA CCTGAATGAC AAGAATGAGT ACAGTGCTTC AACCAAGATG 
GACTACCCTT CTCTAGCACT GCTTGGAGAA AAACTGGCTG AAAACAATAT TTTCCTGATC 
TTCGCCGTTA TAAAAAGACA CTACGTTCTT TACAAGAACC TCACTGCGTT GATCCCTGGG 
ACTACAGTGG AAATTTTGGA CCAGGACTCC AAAAACATCA TTCAACTAAT TGTGAACGCC 
TACAATAACA TAAGGTCAAA AGTTGAGCTG AGCGTGTGGG ATCATCCTGA AGATCTCAGT 
CTGGCCTTCT CCGCTACCTG TCAAGATGGA CAGCCCCTGA CCGGTCTCCG CAAGTGCGCC 
GACCTCAAAA TTGGTGACAC TGTCTCCTTC AATGTAACCG TAGACGCTCG AGGATGTCCT 
CCGAAAGGCA CCAGGAAGAG TTTCACCATC AGGCCTGTTG GTTTTAAAGA CCGTCTAGAG 
GTTTCTGTGG ATTACCGCTG TGATTGCCGC TGCACGTATC ACACAAAGCC GAACAGCAGC 
CGCTGCAACT CTGCTGGCAC TTACAACTGT GGGTTGTGCC GCTGTGAGCC TGGGTATCTG 
GGAGCCCGCT GCGAGTGCCA GGAGGGTGAG GTGGACCACC AGCACCTCAG TGCCTGTCGA 
GAAGCAGAGG GCAAGCAGGT GTGCAGTGGT CGTGGAGAAT GCAGCTGTAA CCAGTGCCTG 
TGCTATGAGT CCGAGTTTGG CAAGATCTAC GGCACCTTCT GCGAATGTGA TGACTTCTCC 
TGTGCCAGAC ACAAGGGCAT ACTCTGCTCA GGTCATGGAG AATGCCACTG TGGTGAGTGT 
AAATGCCATG CCGGTTATAT TGGAGACAAC TGTAATTGCT CGACGGAAAC GGCTTCCTGT 
GTGTCGGATG ATGGCAAGAT CTGTAGCGGC CGGGGCAGCT GTGTGTGCGG ACGCTGCCAG 
TGCACTGAAC CAGGGGCATT TGGGGACACA TGTGAAAAAT GTCCCACCTG TCCAGATGCA 
TGTGGAACAA AGAGGGAATG CATCGAGTGT CGTCTTTTTA ACACAGGAAG ACTGGCTGAT 
AATCAGACCT GTCAGCGCAT GTGCAAGGAC GAAATCACCA CAGTGGAAAC CCTCAAAACT 
GACGATCCCA ACGCTGTTCA GTGTGTGTAT AAGACTGAGA ATGACTGCGT GATGAAGTTT 
ACATACTCTG AACATGCCAA TGGACAATCA GTCCTCACCG CTCTTAAAGA GCCAGAATGT 
GCCGTCGCAC CAAATGCTAT GACCGTGCTG CTGGCTGTGG TTGGCAGTAT CTTGTTGATG 
GGCATCGTGC TACTGGCTCT CTGGAAACTG GTCATCACTG TTCATGACCG CAGGGAGTTT 
GCACGCTTTC AAAGTGCACG TTCACGAGCG CGATATGAGA TGGCATCTAA TCCAGTTTAT 
AAGCGGTCCG TACCAATGGA GACGGATTTC GATATGCATG GTATCAAGTC ACTGAACGGA 
GGAGTCCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITGB5 ( XM_009304631.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302906.1 Danio rerio integrin, beta 5 (itgb5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44109
Clone ID Related Accession (Same CDS sequence)	XM_009304631.1
Accession Version	XM_009304631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	integrin beta-5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAAAC TTTGCTCATC TACACGCCAC CCGGCTCTTC TAGTTTGTAT AGTGTTAGAA 
ATATTTATTC ACAGCGTCAA AGGTCTTAAT GTTTGTATGA GCGGCAGCGC CACGTCTTGT 
GAAGAGTGTC TCCTCATTCA CCCGAGCTGC GCCTGGTGCG CGCAAGAGGA CTTTGGACAG 
GCCAGAACAC TTACCTCAAG ATGTGACTTT AGCCAGAACT TGCAGAAGAG GGGATGCGAT 
GCACCATTTA TAGAGAACCC GAGGAGTGGC AGCTCTGTGC TCCAGAGCAA ACCCTTGAGC 
TACAAAGGGT CAGGAGTGAC CCAGTATGAT GTCATCCAGA TATACCCACA GAAGATCTCT 
CTCAGCTTAA GGCCTGGTGA CCAGGCGTCT TTTGAAGTCC AAGTGCGCCC TGTGGAAGAC 
TATCCTGTTG ATTTGTACTA CCTCATGGAC CTCTCGCTAT CCATGAAGGA CGATCTGGAC 
ACGATCCGCA ACCTGGGCAC CAAGCTGGCT GAAGAAATGA GGAAGCTCAC GAGTAACTTT 
CGTCTTGGGT TTGGTTCCTT TGTGGACAAG AACATCTCAC CCTTCTCCTA CACTGCATCC 
AAGTACCAGG ACAACCCATG CAATGGATAT AAATTGTTCC CAAACTGCGT CCCAACATTT 
GGCTTTCGTC ACCTCTTGTC TCTTACGGAC AAGGTTGATC GTTTCAATGA AGAAGTGCAG 
AAGCAGATGG TTTCGAGAAA CAGGGACGCG CCAGAGGGAG GGTTTGATGC CATTCTTCAG 
GCTGCAGTTT GCAGGGAGAA GATTGGCTGG AGGAAGGAGG CCTATCATCT GCTGGTGTTT 
GCCACTGATG ACGTCCCTCA CTTGGCTTTG GATGGTAAAC TCGGTGGACT GGTGCATCCT 
CATGACGGCC AGTGTCACCT GAATGACAAG AATGAGTACA GTGCTTCAAC CAAGATGGAC 
TATCCTTCTC TGGCACTGCT TGGAGAAAAA CTGGCTGAAA ACAATATTTT CCTGATCTTC 
GCTGTTATAA AAAGACACTA CGTTCTTTAC AAGAACCTCA CTGCGTTGAT CCCTGGGACT 
ACAGTGGAAA TTTTGGACCA GGACTCCAAA AACATCATTC AACTAATTGT GAATGCCTAC 
AATAACATAA GGTCAAAAGT TGAGCTGAGC GTGTGGGATC ATCCTGAAGA TCTCAGTCTG 
GCCTTCTCCG CTACCTGTCA AGATGGACAG CCCCTGACCG GTCTCCGCAA GTGCGCCGAC 
CTCAAAATTG GTGACACTGT CTCCTTCAAT GTAACTGTAG ACGCTCGAGG ATGTCCTCCG 
AAAGGCACCA GGAAGAGTTT CACCATCAGG CCTGTTGGTT TTAAAGACCG TCTAGAGGTT 
TCTGTGGATT ACCGCTGTGA TTGCCGCTGC ACGTATCACA CAAAGCCGAA CAGCAGCCGC 
TGCAACTCTG CTGGCACTTA CAACTGTGGG TTGTGCCGGT GTGAGCCTGG GTATCTGGGA 
GCCCGCTGCG AGTGCCAGGA GGGTGAGGTG GACCACCAGC ACCTCAGTGC CTGTCGAGAA 
GCAGAGGGCA AGCAGGTGTG CAGTGGTCGT GGAGAATGCA GCTGTAACCA GTGCCTGTGC 
TATGAGTCAG AGTTTGGCAA GATCTACGGC ACCTTCTGCG AATGTGACGA CTTCTCCTGT 
GCCCGACACA AGGGCATACT CTGCTCAGGT CATGGAGAAT GCCACTGTGG TGAGTGTAAA 
TGCCATGCCG GTTATATTGG AGACAACTGT AATTGCTCGA CGGAAACGGC TTCCTGTGTG 
TCGGATGATG GCAAGATCTG TAGCGGCCGG GGCAGCTGTG TGTGCGGACG CTGCCAGTGC 
ACTGAACCAG GGGCATTTGG GGACACATGT GAAAAATGTC CCACCTGTCC AGATGCATGT 
GGAACAAAGA GGGAATGCAT CGAGTGTCGT CTTTTTAACA CAGGAAGACT GGCTGATAAT 
CAGACCTGTC AGCGCATGTG CAAGGACGAA ATCACCACAG TGGAAACCCT CAAAACTGAC 
GATCCCAACG CTGTTCAGTG TGTGTATAAG ACTGAGAATG ACTGCGTGAT GAAGTTTACA 
TACTCTGAAC ATGCCAATGG ACAATCAGTC CTCACCGCTC TTAAAGAGCC AGAATGTGCC 
GTCGCACCAA ATGCTATGAC CGTGCTGCTG GCTGTGGTTG GCAGTATCTT GTTGATGGGC 
ATCGTGCTAC TGGCTCTCTG GAAACTGGTC ATCACTGTTC ATGACCGCAG GGAGTTTGCA 
CGCTTTCAAA GTGCACGTTC ACGAGCGCGA TATGAGATGG CATCTAATCC AGTTTATAAG 
CGGTCCGTAC CAATGGAGAC GGATTTCGAT ATGCATGGTA TCAAGTCACT GAACGGAGGA 
GTCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302906.1
CDS	459..2867
Translation	MWKLCSSTRHPALLVCIVLEIFIHSVKGLNVCMSGSATSCEECLLIHPSCAWCAQEDFGQARTLTSRCDFSQNLQKRGCDAPFIENPRSGSSVLQSKPLSYKGSGVTQYDVIQIYPQKISLSLRPGDQASFEVQVRPVEDYPVDLYYLMDLSLSMKDDLDTIRNLGTKLAEEMRKLTSNFRLGFGSFVDKNISPFSYTASKYQDNPCNGYKLFPNCVPTFGFRHLLSLTDKVDRFNEEVQKQMVSRNRDAPEGGFDAILQAAVCREKIGWRKEAYHLLVFATDDVPHLALDGKLGGLVHPHDGQCHLNDKNEYSASTKMDYPSLALLGEKLAENNIFLIFAVIKRHYVLYKNLTALIPGTTVEILDQDSKNIIQLIVNAYNNIRSKVELSVWDHPEDLSLAFSATCQDGQPLTGLRKCADLKIGDTVSFNVTVDARGCPPKGTRKSFTIRPVGFKDRLEVSVDYRCDCRCTYHTKPNSSRCNSAGTYNCGLCRCEPGYLGARCECQEGEVDHQHLSACREAEGKQVCSGRGECSCNQCLCYESEFGKIYGTFCECDDFSCARHKGILCSGHGECHCGECKCHAGYIGDNCNCSTETASCVSDDGKICSGRGSCVCGRCQCTEPGAFGDTCEKCPTCPDACGTKRECIECRLFNTGRLADNQTCQRMCKDEITTVETLKTDDPNAVQCVYKTENDCVMKFTYSEHANGQSVLTALKEPECAVAPNAMTVLLAVVGSILLMGIVLLALWKLVITVHDRREFARFQSARSRARYEMASNPVYKRSVPMETDFDMHGIKSLNGGVH

Target ORF information:

RefSeq Version	XM_009304631.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio integrin, beta 5 (itgb5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304631.1

ORF Insert Sequence:

ATGTGGAAAC TTTGCTCATC TACACGCCAC CCGGCTCTTC TAGTTTGTAT AGTGTTAGAA 
ATATTTATTC ACAGCGTCAA AGGTCTTAAT GTTTGTATGA GCGGCAGCGC CACGTCTTGT 
GAAGAGTGTC TCCTCATTCA CCCGAGCTGC GCCTGGTGCG CGCAAGAGGA CTTTGGACAG 
GCCAGAACAC TTACCTCAAG ATGTGACTTT AGCCAGAACT TGCAGAAGAG GGGATGCGAT 
GCACCATTTA TAGAGAACCC GAGGAGTGGC AGCTCTGTGC TCCAGAGCAA ACCCTTGAGC 
TACAAAGGGT CAGGAGTGAC CCAGTATGAT GTCATCCAGA TATACCCACA GAAGATCTCT 
CTCAGCTTAA GGCCTGGTGA CCAGGCGTCT TTTGAAGTCC AAGTGCGCCC TGTGGAAGAC 
TATCCTGTTG ATTTGTACTA CCTCATGGAC CTCTCGCTAT CCATGAAGGA CGATCTGGAC 
ACGATCCGCA ACCTGGGCAC CAAGCTGGCT GAAGAAATGA GGAAGCTCAC GAGTAACTTT 
CGTCTTGGGT TTGGTTCCTT TGTGGACAAG AACATCTCAC CCTTCTCCTA CACTGCATCC 
AAGTACCAGG ACAACCCATG CAATGGATAT AAATTGTTCC CAAACTGCGT CCCAACATTT 
GGCTTTCGTC ACCTCTTGTC TCTTACGGAC AAGGTTGATC GTTTCAATGA AGAAGTGCAG 
AAGCAGATGG TTTCGAGAAA CAGGGACGCG CCAGAGGGAG GGTTTGATGC CATTCTTCAG 
GCTGCAGTTT GCAGGGAGAA GATTGGCTGG AGGAAGGAGG CCTATCATCT GCTGGTGTTT 
GCCACTGATG ACGTCCCTCA CTTGGCTTTG GATGGTAAAC TCGGTGGACT GGTGCATCCT 
CATGACGGCC AGTGTCACCT GAATGACAAG AATGAGTACA GTGCTTCAAC CAAGATGGAC 
TATCCTTCTC TGGCACTGCT TGGAGAAAAA CTGGCTGAAA ACAATATTTT CCTGATCTTC 
GCTGTTATAA AAAGACACTA CGTTCTTTAC AAGAACCTCA CTGCGTTGAT CCCTGGGACT 
ACAGTGGAAA TTTTGGACCA GGACTCCAAA AACATCATTC AACTAATTGT GAATGCCTAC 
AATAACATAA GGTCAAAAGT TGAGCTGAGC GTGTGGGATC ATCCTGAAGA TCTCAGTCTG 
GCCTTCTCCG CTACCTGTCA AGATGGACAG CCCCTGACCG GTCTCCGCAA GTGCGCCGAC 
CTCAAAATTG GTGACACTGT CTCCTTCAAT GTAACTGTAG ACGCTCGAGG ATGTCCTCCG 
AAAGGCACCA GGAAGAGTTT CACCATCAGG CCTGTTGGTT TTAAAGACCG TCTAGAGGTT 
TCTGTGGATT ACCGCTGTGA TTGCCGCTGC ACGTATCACA CAAAGCCGAA CAGCAGCCGC 
TGCAACTCTG CTGGCACTTA CAACTGTGGG TTGTGCCGGT GTGAGCCTGG GTATCTGGGA 
GCCCGCTGCG AGTGCCAGGA GGGTGAGGTG GACCACCAGC ACCTCAGTGC CTGTCGAGAA 
GCAGAGGGCA AGCAGGTGTG CAGTGGTCGT GGAGAATGCA GCTGTAACCA GTGCCTGTGC 
TATGAGTCAG AGTTTGGCAA GATCTACGGC ACCTTCTGCG AATGTGACGA CTTCTCCTGT 
GCCCGACACA AGGGCATACT CTGCTCAGGT CATGGAGAAT GCCACTGTGG TGAGTGTAAA 
TGCCATGCCG GTTATATTGG AGACAACTGT AATTGCTCGA CGGAAACGGC TTCCTGTGTG 
TCGGATGATG GCAAGATCTG TAGCGGCCGG GGCAGCTGTG TGTGCGGACG CTGCCAGTGC 
ACTGAACCAG GGGCATTTGG GGACACATGT GAAAAATGTC CCACCTGTCC AGATGCATGT 
GGAACAAAGA GGGAATGCAT CGAGTGTCGT CTTTTTAACA CAGGAAGACT GGCTGATAAT 
CAGACCTGTC AGCGCATGTG CAAGGACGAA ATCACCACAG TGGAAACCCT CAAAACTGAC 
GATCCCAACG CTGTTCAGTG TGTGTATAAG ACTGAGAATG ACTGCGTGAT GAAGTTTACA 
TACTCTGAAC ATGCCAATGG ACAATCAGTC CTCACCGCTC TTAAAGAGCC AGAATGTGCC 
GTCGCACCAA ATGCTATGAC CGTGCTGCTG GCTGTGGTTG GCAGTATCTT GTTGATGGGC 
ATCGTGCTAC TGGCTCTCTG GAAACTGGTC ATCACTGTTC ATGACCGCAG GGAGTTTGCA 
CGCTTTCAAA GTGCACGTTC ACGAGCGCGA TATGAGATGG CATCTAATCC AGTTTATAAG 
CGGTCCGTAC CAATGGAGAC GGATTTCGAT ATGCATGGTA TCAAGTCACT GAACGGAGGA 
GTCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activation of Hepatic Stellate Cells During Liver Carcinogenesis Requires Fibrinogen/Integrin αvβ5 in Zebrafish.
Neoplasia (New York, N.Y.)20(5)533-542(2018 05)
Yan C,Yang Q,Gong Z

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Integrin alphaV is necessary for gastrulation movements that regulate vertebrate body asymmetry.
Development (Cambridge, England)137(20)3449-58(2010 Oct)
Ablooglu AJ,Tkachenko E,Kang J,Shattil SJ

MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis.
Nature464(7292)1196-200(2010 Apr)
Nicoli S,Standley C,Walker P,Hurlstone A,Fogarty KE,Lawson ND

Nlcam modulates midline convergence during anterior neural plate morphogenesis.
Developmental biology339(1)14-25(2010 Mar)
Brown KE,Keller PJ,Ramialison M,Rembold M,Stelzer EH,Loosli F,Wittbrodt J

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ITGB5 cDNA ORF clone, Danio rerio(Zebrafish)

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