THBS1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	THBS1B
Entrez Gene ID	561901
Full Name	thrombospondin 1b
Synonyms	Tsp-1,si:dkey-11e23.1,thbs1,wu:fc48c03
General protein information	Preferred Names thrombospondin 1b Names thrombospondin-1 thrombospondin 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160762.4	XP_005160819.1	thrombospondin-1 isoform X1
XM_685303.9	XP_690395.4	thrombospondin-1 isoform X2
XM_005160762.3	XP_005160819.1	thrombospondin-1 isoform X1
XM_685303.8	XP_690395.4	thrombospondin-1 isoform X2

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	THBS1	XP_001093770.2
Mus musculus (house mouse)	Thbs1	NP_035710.2
Gallus gallus (chicken)	THBS1	NP_001186382.1
Pan troglodytes (chimpanzee)	THBS1	XP_510294.3
Canis lupus familiaris (dog)	THBS1	XP_544610.3
Bos taurus (cattle)	THBS1	NP_776621.1
Rattus norvegicus (Norway rat)	Thbs1	NP_001013080.1
Homo sapiens (human)	THBS1	NP_003237.2
Danio rerio (zebrafish)	thbs1	XP_005160819.1
Xenopus tropicalis (tropical clawed frog)	thbs1	XP_002937245.1

Gene Ontology

The following THBS1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THBS1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa25039	XM_005160762.4 Latest version!	Danio rerio thrombospondin 1b (thbs1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa25040	XM_685303.9 Latest version!	Danio rerio thrombospondin 1b (thbs1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa25039	XM_005160762.3	Danio rerio thrombospondin 1b (thbs1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa25040	XM_685303.8	Danio rerio thrombospondin 1b (thbs1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THBS1B ( XM_005160762.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160819.1 Danio rerio thrombospondin 1b (thbs1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25039
Clone ID Related Accession (Same CDS sequence)	XM_005160762.4 , XM_005160762.3
Accession Version	XM_005160762.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	thrombospondin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160762.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG 
AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC 
CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC 
ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG 
GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC 
AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG 
TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA 
GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC 
TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA 
GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC 
ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAGCAATGAC TGATATCATT 
ACCCTTGACA ATCCCATAAA TGGATCCAGC CCTGCAATCA GGACAGATTA CACTGGCCAC 
AAAACAAAAG ATCTGCAAAT GATTTGTGGC TTTTCATGTG AGGATCTGGC TGCCATGTTT 
AAGGAACTGA AAGGTCTTGG TGTGGTGGTT CAAGAACTGT CCAATGAACT CCGAAAAGTG 
ACGGATGACA AAAACATGCT CATGAATCAA ATGGGCATTC GTGCTGGTGT ATGTCTTCAC 
AATGGAATTG TGCACAAAAA CAAGGAGGAA TGGACAGTGG ACGACTGCAC AGAGTGCACT 
TGCCAGAATT CTGCAACGGT GTGCCGCAAG ATTTCATGTC CCCTAATCCC ATGTGCAAAT 
GCCACAGTAC CTGATGGAGA GTGCTGCCCT CGCTGTGGAA CACCGAGCGA CTCCGCCGAG 
GATGGTTGGT CCCCCTGGTC TGAATGGACC CATTGTTCAG TGTCCTGTGG AAGGGGCATT 
CAGCAGCGCG GCCGTTCCTG CGACCGCATC AATAACAACT GTGAGGGCAC GTCAGTGCAG 
ACCAGAGACT GCTACCTCCA GGAGTGCGAC AAGCGCTTTA AGCAAGACGG TAGCTGGAGC 
CACTGGTCAC CCTGGTCATC GTGTTCGGTC ACATGTGGTG CTGGTGTTAT TACACGCATC 
CGTCTCTGTA ACTCCCCGAC ACCTCAAATG GATGGCAAAG ACTGCCAGGG TGAAGGGCGG 
CAAACTGAGA GGTGTGAGAA ATCACCATGT CCGATCAACG GTGGCTGGGG ACCATGGTCG 
CCCTGGGATA CTTGCTCTGT TACCTGTGGT GGTGGCGTCC AAAACCGTAA GCGTCTTTGC 
AATAATCCTG TACCCAAACA TGGTGGCAAA GAGTGTGTGG GAGATGCAAA GGTCAGCCAG 
ATTTGCAACA AACAAGCCTG TCCTGTTGAT GGATGTCTTT CGAGTCCGTG CTTTGAAGGG 
GCCCAGTGCA CAAGCTTTCC AGATGGGTCC TGGAAATGTG GAAAATGCCC CACAGGATAC 
ACTGGCAATG GAATCAACTG CAAAGATGTC AATGAGTGTA AGGAAGTACC AGATGCCTGC 
TTTGAATTTA ATGGAGTCCA CAGATGTGAG AACACAGTGC CAGGCTACAA CTGTCTCCCT 
TGCCCCACTC GTTACACCGG GCCACAGCCG TTTGGCCGTG GAGTGGAGGA CGCCGCTGCT 
AAGAAGCAGG TGTGCACTCC TCGAAATCCC TGCCTGGATG GAAGCCACGA CTGCAATAAA 
AACGCTCGCT GCAACTACCT GGGCCATTTC TCTGACCCCA TGTTCCGCTG TGAATGCAAA 
CCTGGCTTTG CTGGAAATGG ACACATCTGT GGAGAAGATA CTGATCTTGA TGGTTGGCCG 
AATGCTGATC TCGTGTGTGT TGAGAACGCA ACCTATCACT GCAAGAAGGA CAACTGCCCA 
AATCTTCCCA ACTCAGGGCA AGAAGACTAT GACAAGGATG GCATTGGTGA TGCATGTGAC 
GATGATGATG ATGACGATGG CATTCCTGAT GACAGGGATA ACTGCCCACT CGTCTTCAAC 
CCGAGACAGT ACGACTATGA TCGGGATGAT GTTGGTGACC GTTGTGATAA CTGTCCCTAC 
AACAGCAACC CAGACCAGAC AGACACTGAC AACAATGGAG AAGGAGACGC CTGTGCTGTG 
GACATTGATG GAGATGGTAT TCTGAACGAG AAAGACAACT GTCCGTATCT GTACAACGTC 
GACCAGAAAG ACACTGATCT TGATGGAGTT GGAGACCAGT GTGACAACTG TCCATTGGAA 
CACAACCCAG ATCAGCTGGA CTCTGATTCT GACCGCGTTG GAGACAAATG TGACAGTAAC 
CAGGATATCG ATGAAGACGG TCACCAGAAC AACCTGGACA ACTGTCCATA CATCCCCAAT 
GCCAACCAGG CTGATCATGA CAAGGACGGT AAAGGAGATG CCTGCGACTA CGATGATGAT 
AATGATGGCA TTCCTGACGA AAAAGACAAC TGCAGACTGG TCTTCAATCC AGATCAGCTA 
GATTCTGATG GCGATGGACG TGGCGATGCC TGTAAAGATG ACTTTGACAT GGACAATGTT 
CTGGATATCT ATGATGTCTG TCCTGAGAAC TTCGATATCA GTGAAACCGA CTTCCGCAAG 
TTCCAGATGG TTCCTCTGGA TCCCAAGGGC ACTTCTCAGA TCGATCCCAA CTGGGTGGTT 
CGTCATCAAG GAAAAGAGCT GGTTCAGACA GTCAACTGCG ACCCTGGCAT TGCTGTTGGT 
TTTGATGAGT TCAATTCAGT GGACTTCAGT GGAACATTCT TCATCAACAC TGAGAGGGAT 
GACGACTATG CTGGTTTTGT TTTCGGATAC CAGTCCAGCT CTCGCTTCTA TGTGGTCATG 
TGGAAGCAGA TCACACAGAC CTACTGGTCC AGCACACCTA CCAAAGCACA GGGTTACTCA 
GGGCTGTCCA TCAAAGTGGT TAATTCCACC ACAGGACCTG GGGAGCATCT GAGAAACGCC 
CTTTGGCACA CTGGAGACAC ACCAGGACAG GTGCGCACAT TGTGGCATGA CCCAAAGAAT 
GTGGGATGGA AGGACTTTAC TGCTTACAGA TGGCACCTGA CACACAGACC AAGAACTGGA 
CACATAAGAG TGGTCATGTA TGAAGGCAAA AAGATCATGG CAGATTCTGG CAGAATCTAC 
GACAAAACAT ACGCAGGCGG AAGACTAGGT CTCTTCGTCT TCTCACAAGA GATGGTGTAT 
TTCTCAGACC TCAAATACGA ATGCAGAGAT GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160819.1
CDS	187..3702
Translation	MKSTAIFLLLMLWNCESARVAESRDDNSVYDLFELVQVPRKNHGVTLVKGDDPYSPAYKILNPDLIPPVPESAFRDLIDSIHAEKGFLLLVNFKQFKRTRGSLLTVEKNDGSGPVFEIVSNGKANTLDIVFSTENKQQVVSIEEADLAVGHWKNITLFVQEDRVQFYVGCEEVNVAELDASIHTILTQEIPGVAKMRIGKGAVKDRFMGVLQNVRFVFGTTLEAILRNKGCQNSAMTDIITLDNPINGSSPAIRTDYTGHKTKDLQMICGFSCEDLAAMFKELKGLGVVVQELSNELRKVTDDKNMLMNQMGIRAGVCLHNGIVHKNKEEWTVDDCTECTCQNSATVCRKISCPLIPCANATVPDGECCPRCGTPSDSAEDGWSPWSEWTHCSVSCGRGIQQRGRSCDRINNNCEGTSVQTRDCYLQECDKRFKQDGSWSHWSPWSSCSVTCGAGVITRIRLCNSPTPQMDGKDCQGEGRQTERCEKSPCPINGGWGPWSPWDTCSVTCGGGVQNRKRLCNNPVPKHGGKECVGDAKVSQICNKQACPVDGCLSSPCFEGAQCTSFPDGSWKCGKCPTGYTGNGINCKDVNECKEVPDACFEFNGVHRCENTVPGYNCLPCPTRYTGPQPFGRGVEDAAAKKQVCTPRNPCLDGSHDCNKNARCNYLGHFSDPMFRCECKPGFAGNGHICGEDTDLDGWPNADLVCVENATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDDDGIPDDRDNCPLVFNPRQYDYDRDDVGDRCDNCPYNSNPDQTDTDNNGEGDACAVDIDGDGILNEKDNCPYLYNVDQKDTDLDGVGDQCDNCPLEHNPDQLDSDSDRVGDKCDSNQDIDEDGHQNNLDNCPYIPNANQADHDKDGKGDACDYDDDNDGIPDEKDNCRLVFNPDQLDSDGDGRGDACKDDFDMDNVLDIYDVCPENFDISETDFRKFQMVPLDPKGTSQIDPNWVVRHQGKELVQTVNCDPGIAVGFDEFNSVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQITQTYWSSTPTKAQGYSGLSIKVVNSTTGPGEHLRNALWHTGDTPGQVRTLWHDPKNVGWKDFTAYRWHLTHRPRTGHIRVVMYEGKKIMADSGRIYDKTYAGGRLGLFVFSQEMVYFSDLKYECRDA

Target ORF information:

RefSeq Version	XM_005160762.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin 1b (thbs1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160762.4

ORF Insert Sequence:

ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG 
AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC 
CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC 
ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG 
GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC 
AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG 
TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA 
GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC 
TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA 
GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC 
ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAGCAATGAC TGATATCATT 
ACCCTTGACA ATCCCATAAA TGGATCCAGC CCTGCAATCA GGACAGATTA CACTGGCCAC 
AAAACAAAAG ATCTGCAAAT GATTTGTGGC TTTTCATGTG AGGATCTGGC TGCCATGTTT 
AAGGAACTGA AAGGTCTTGG TGTGGTGGTT CAAGAACTGT CCAATGAACT CCGAAAAGTG 
ACGGATGACA AAAACATGCT CATGAATCAA ATGGGCATTC GTGCTGGTGT ATGTCTTCAC 
AATGGAATTG TGCACAAAAA CAAGGAGGAA TGGACAGTGG ACGACTGCAC AGAGTGCACT 
TGCCAGAATT CTGCAACGGT GTGCCGCAAG ATTTCATGTC CCCTAATCCC ATGTGCAAAT 
GCCACAGTAC CTGATGGAGA GTGCTGCCCT CGCTGTGGAA CACCGAGCGA CTCCGCCGAG 
GATGGTTGGT CCCCCTGGTC TGAATGGACC CATTGTTCAG TGTCCTGTGG AAGGGGCATT 
CAGCAGCGCG GCCGTTCCTG CGACCGCATC AATAACAACT GTGAGGGCAC GTCAGTGCAG 
ACCAGAGACT GCTACCTCCA GGAGTGCGAC AAGCGCTTTA AGCAAGACGG TAGCTGGAGC 
CACTGGTCAC CCTGGTCATC GTGTTCGGTC ACATGTGGTG CTGGTGTTAT TACACGCATC 
CGTCTCTGTA ACTCCCCGAC ACCTCAAATG GATGGCAAAG ACTGCCAGGG TGAAGGGCGG 
CAAACTGAGA GGTGTGAGAA ATCACCATGT CCGATCAACG GTGGCTGGGG ACCATGGTCG 
CCCTGGGATA CTTGCTCTGT TACCTGTGGT GGTGGCGTCC AAAACCGTAA GCGTCTTTGC 
AATAATCCTG TACCCAAACA TGGTGGCAAA GAGTGTGTGG GAGATGCAAA GGTCAGCCAG 
ATTTGCAACA AACAAGCCTG TCCTGTTGAT GGATGTCTTT CGAGTCCGTG CTTTGAAGGG 
GCCCAGTGCA CAAGCTTTCC AGATGGGTCC TGGAAATGTG GAAAATGCCC CACAGGATAC 
ACTGGCAATG GAATCAACTG CAAAGATGTC AATGAGTGTA AGGAAGTACC AGATGCCTGC 
TTTGAATTTA ATGGAGTCCA CAGATGTGAG AACACAGTGC CAGGCTACAA CTGTCTCCCT 
TGCCCCACTC GTTACACCGG GCCACAGCCG TTTGGCCGTG GAGTGGAGGA CGCCGCTGCT 
AAGAAGCAGG TGTGCACTCC TCGAAATCCC TGCCTGGATG GAAGCCACGA CTGCAATAAA 
AACGCTCGCT GCAACTACCT GGGCCATTTC TCTGACCCCA TGTTCCGCTG TGAATGCAAA 
CCTGGCTTTG CTGGAAATGG ACACATCTGT GGAGAAGATA CTGATCTTGA TGGTTGGCCG 
AATGCTGATC TCGTGTGTGT TGAGAACGCA ACCTATCACT GCAAGAAGGA CAACTGCCCA 
AATCTTCCCA ACTCAGGGCA AGAAGACTAT GACAAGGATG GCATTGGTGA TGCATGTGAC 
GATGATGATG ATGACGATGG CATTCCTGAT GACAGGGATA ACTGCCCACT CGTCTTCAAC 
CCGAGACAGT ACGACTATGA TCGGGATGAT GTTGGTGACC GTTGTGATAA CTGTCCCTAC 
AACAGCAACC CAGACCAGAC AGACACTGAC AACAATGGAG AAGGAGACGC CTGTGCTGTG 
GACATTGATG GAGATGGTAT TCTGAACGAG AAAGACAACT GTCCGTATCT GTACAACGTC 
GACCAGAAAG ACACTGATCT TGATGGAGTT GGAGACCAGT GTGACAACTG TCCATTGGAA 
CACAACCCAG ATCAGCTGGA CTCTGATTCT GACCGCGTTG GAGACAAATG TGACAGTAAC 
CAGGATATCG ATGAAGACGG TCACCAGAAC AACCTGGACA ACTGTCCATA CATCCCCAAT 
GCCAACCAGG CTGATCATGA CAAGGACGGT AAAGGAGATG CCTGCGACTA CGATGATGAT 
AATGATGGCA TTCCTGACGA AAAAGACAAC TGCAGACTGG TCTTCAATCC AGATCAGCTA 
GATTCTGATG GCGATGGACG TGGCGATGCC TGTAAAGATG ACTTTGACAT GGACAATGTT 
CTGGATATCT ATGATGTCTG TCCTGAGAAC TTCGATATCA GTGAAACCGA CTTCCGCAAG 
TTCCAGATGG TTCCTCTGGA TCCCAAGGGC ACTTCTCAGA TCGATCCCAA CTGGGTGGTT 
CGTCATCAAG GAAAAGAGCT GGTTCAGACA GTCAACTGCG ACCCTGGCAT TGCTGTTGGT 
TTTGATGAGT TCAATTCAGT GGACTTCAGT GGAACATTCT TCATCAACAC TGAGAGGGAT 
GACGACTATG CTGGTTTTGT TTTCGGATAC CAGTCCAGCT CTCGCTTCTA TGTGGTCATG 
TGGAAGCAGA TCACACAGAC CTACTGGTCC AGCACACCTA CCAAAGCACA GGGTTACTCA 
GGGCTGTCCA TCAAAGTGGT TAATTCCACC ACAGGACCTG GGGAGCATCT GAGAAACGCC 
CTTTGGCACA CTGGAGACAC ACCAGGACAG GTGCGCACAT TGTGGCATGA CCCAAAGAAT 
GTGGGATGGA AGGACTTTAC TGCTTACAGA TGGCACCTGA CACACAGACC AAGAACTGGA 
CACATAAGAG TGGTCATGTA TGAAGGCAAA AAGATCATGG CAGATTCTGG CAGAATCTAC 
GACAAAACAT ACGCAGGCGG AAGACTAGGT CTCTTCGTCT TCTCACAAGA GATGGTGTAT 
TTCTCAGACC TCAAATACGA ATGCAGAGAT GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1B ( XM_005160762.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160819.1 Danio rerio thrombospondin 1b (thbs1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25039
Clone ID Related Accession (Same CDS sequence)	XM_005160762.4 , XM_005160762.3
Accession Version	XM_005160762.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	thrombospondin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160762.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG 
AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC 
CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC 
ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG 
GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC 
AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG 
TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA 
GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC 
TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA 
GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC 
ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAGCAATGAC TGATATCATT 
ACCCTTGACA ATCCCATAAA TGGATCCAGC CCTGCAATCA GGACAGATTA CACTGGCCAC 
AAAACAAAAG ATCTGCAAAT GATTTGTGGC TTTTCATGTG AGGATCTGGC TGCCATGTTT 
AAGGAACTGA AAGGTCTTGG TGTGGTGGTT CAAGAACTGT CCAATGAACT CCGAAAAGTG 
ACGGATGACA AAAACATGCT CATGAATCAA ATGGGCATTC GTGCTGGTGT ATGTCTTCAC 
AATGGAATTG TGCACAAAAA CAAGGAGGAA TGGACAGTGG ACGACTGCAC AGAGTGCACT 
TGCCAGAATT CTGCAACGGT GTGCCGCAAG ATTTCATGTC CCCTAATCCC ATGTGCAAAT 
GCCACAGTAC CTGATGGAGA GTGCTGCCCT CGCTGTGGAA CACCGAGCGA CTCCGCCGAG 
GATGGTTGGT CCCCCTGGTC TGAATGGACC CATTGTTCAG TGTCCTGTGG AAGGGGCATT 
CAGCAGCGCG GCCGTTCCTG CGACCGCATC AATAACAACT GTGAGGGCAC GTCAGTGCAG 
ACCAGAGACT GCTACCTCCA GGAGTGCGAC AAGCGCTTTA AGCAAGACGG TAGCTGGAGC 
CACTGGTCAC CCTGGTCATC GTGTTCGGTC ACATGTGGTG CTGGTGTTAT TACACGCATC 
CGTCTCTGTA ACTCCCCGAC ACCTCAAATG GATGGCAAAG ACTGCCAGGG TGAAGGGCGG 
CAAACTGAGA GGTGTGAGAA ATCACCATGT CCGATCAACG GTGGCTGGGG ACCATGGTCG 
CCCTGGGATA CTTGCTCTGT TACCTGTGGT GGTGGCGTCC AAAACCGTAA GCGTCTTTGC 
AATAATCCTG TACCCAAACA TGGTGGCAAA GAGTGTGTGG GAGATGCAAA GGTCAGCCAG 
ATTTGCAACA AACAAGCCTG TCCTGTTGAT GGATGTCTTT CGAGTCCGTG CTTTGAAGGG 
GCCCAGTGCA CAAGCTTTCC AGATGGGTCC TGGAAATGTG GAAAATGCCC CACAGGATAC 
ACTGGCAATG GAATCAACTG CAAAGATGTC AATGAGTGTA AGGAAGTACC AGATGCCTGC 
TTTGAATTTA ATGGAGTCCA CAGATGTGAG AACACAGTGC CAGGCTACAA CTGTCTCCCT 
TGCCCCACTC GTTACACCGG GCCACAGCCG TTTGGCCGTG GAGTGGAGGA CGCCGCTGCT 
AAGAAGCAGG TGTGCACTCC TCGAAATCCC TGCCTGGATG GAAGCCACGA CTGCAATAAA 
AACGCTCGCT GCAACTACCT GGGCCATTTC TCTGACCCCA TGTTCCGCTG TGAATGCAAA 
CCTGGCTTTG CTGGAAATGG ACACATCTGT GGAGAAGATA CTGATCTTGA TGGTTGGCCG 
AATGCTGATC TCGTGTGTGT TGAGAACGCA ACCTATCACT GCAAGAAGGA CAACTGCCCA 
AATCTTCCCA ACTCAGGGCA AGAAGACTAT GACAAGGATG GCATTGGTGA TGCATGTGAC 
GATGATGATG ATGACGATGG CATTCCTGAT GACAGGGATA ACTGCCCACT CGTCTTCAAC 
CCGAGACAGT ACGACTATGA TCGGGATGAT GTTGGTGACC GTTGTGATAA CTGTCCCTAC 
AACAGCAACC CAGACCAGAC AGACACTGAC AACAATGGAG AAGGAGACGC CTGTGCTGTG 
GACATTGATG GAGATGGTAT TCTGAACGAG AAAGACAACT GTCCGTATCT GTACAACGTC 
GACCAGAAAG ACACTGATCT TGATGGAGTT GGAGACCAGT GTGACAACTG TCCATTGGAA 
CACAACCCAG ATCAGCTGGA CTCTGATTCT GACCGCGTTG GAGACAAATG TGACAGTAAC 
CAGGATATCG ATGAAGACGG TCACCAGAAC AACCTGGACA ACTGTCCATA CATCCCCAAT 
GCCAACCAGG CTGATCATGA CAAGGACGGT AAAGGAGATG CCTGCGACTA CGATGATGAT 
AATGATGGCA TTCCTGACGA AAAAGACAAC TGCAGACTGG TCTTCAATCC AGATCAGCTA 
GATTCTGATG GCGATGGACG TGGCGATGCC TGTAAAGATG ACTTTGACAT GGACAATGTT 
CTGGATATCT ATGATGTCTG TCCTGAGAAC TTCGATATCA GTGAAACCGA CTTCCGCAAG 
TTCCAGATGG TTCCTCTGGA TCCCAAGGGC ACTTCTCAGA TCGATCCCAA CTGGGTGGTT 
CGTCATCAAG GAAAAGAGCT GGTTCAGACA GTCAACTGCG ACCCTGGCAT TGCTGTTGGT 
TTTGATGAGT TCAATTCAGT GGACTTCAGT GGAACATTCT TCATCAACAC TGAGAGGGAT 
GACGACTATG CTGGTTTTGT TTTCGGATAC CAGTCCAGCT CTCGCTTCTA TGTGGTCATG 
TGGAAGCAGA TCACACAGAC CTACTGGTCC AGCACACCTA CCAAAGCACA GGGTTACTCA 
GGGCTGTCCA TCAAAGTGGT TAATTCCACC ACAGGACCTG GGGAGCATCT GAGAAACGCC 
CTTTGGCACA CTGGAGACAC ACCAGGACAG GTGCGCACAT TGTGGCATGA CCCAAAGAAT 
GTGGGATGGA AGGACTTTAC TGCTTACAGA TGGCACCTGA CACACAGACC AAGAACTGGA 
CACATAAGAG TGGTCATGTA TGAAGGCAAA AAGATCATGG CAGATTCTGG CAGAATCTAC 
GACAAAACAT ACGCAGGCGG AAGACTAGGT CTCTTCGTCT TCTCACAAGA GATGGTGTAT 
TTCTCAGACC TCAAATACGA ATGCAGAGAT GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160819.1
CDS	191..3706
Translation	MKSTAIFLLLMLWNCESARVAESRDDNSVYDLFELVQVPRKNHGVTLVKGDDPYSPAYKILNPDLIPPVPESAFRDLIDSIHAEKGFLLLVNFKQFKRTRGSLLTVEKNDGSGPVFEIVSNGKANTLDIVFSTENKQQVVSIEEADLAVGHWKNITLFVQEDRVQFYVGCEEVNVAELDASIHTILTQEIPGVAKMRIGKGAVKDRFMGVLQNVRFVFGTTLEAILRNKGCQNSAMTDIITLDNPINGSSPAIRTDYTGHKTKDLQMICGFSCEDLAAMFKELKGLGVVVQELSNELRKVTDDKNMLMNQMGIRAGVCLHNGIVHKNKEEWTVDDCTECTCQNSATVCRKISCPLIPCANATVPDGECCPRCGTPSDSAEDGWSPWSEWTHCSVSCGRGIQQRGRSCDRINNNCEGTSVQTRDCYLQECDKRFKQDGSWSHWSPWSSCSVTCGAGVITRIRLCNSPTPQMDGKDCQGEGRQTERCEKSPCPINGGWGPWSPWDTCSVTCGGGVQNRKRLCNNPVPKHGGKECVGDAKVSQICNKQACPVDGCLSSPCFEGAQCTSFPDGSWKCGKCPTGYTGNGINCKDVNECKEVPDACFEFNGVHRCENTVPGYNCLPCPTRYTGPQPFGRGVEDAAAKKQVCTPRNPCLDGSHDCNKNARCNYLGHFSDPMFRCECKPGFAGNGHICGEDTDLDGWPNADLVCVENATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDDDGIPDDRDNCPLVFNPRQYDYDRDDVGDRCDNCPYNSNPDQTDTDNNGEGDACAVDIDGDGILNEKDNCPYLYNVDQKDTDLDGVGDQCDNCPLEHNPDQLDSDSDRVGDKCDSNQDIDEDGHQNNLDNCPYIPNANQADHDKDGKGDACDYDDDNDGIPDEKDNCRLVFNPDQLDSDGDGRGDACKDDFDMDNVLDIYDVCPENFDISETDFRKFQMVPLDPKGTSQIDPNWVVRHQGKELVQTVNCDPGIAVGFDEFNSVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQITQTYWSSTPTKAQGYSGLSIKVVNSTTGPGEHLRNALWHTGDTPGQVRTLWHDPKNVGWKDFTAYRWHLTHRPRTGHIRVVMYEGKKIMADSGRIYDKTYAGGRLGLFVFSQEMVYFSDLKYECRDA

Target ORF information:

RefSeq Version	XM_005160762.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio thrombospondin 1b (thbs1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160762.3

ORF Insert Sequence:

ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG 
AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC 
CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC 
ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG 
GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC 
AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG 
TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA 
GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC 
TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA 
GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC 
ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAGCAATGAC TGATATCATT 
ACCCTTGACA ATCCCATAAA TGGATCCAGC CCTGCAATCA GGACAGATTA CACTGGCCAC 
AAAACAAAAG ATCTGCAAAT GATTTGTGGC TTTTCATGTG AGGATCTGGC TGCCATGTTT 
AAGGAACTGA AAGGTCTTGG TGTGGTGGTT CAAGAACTGT CCAATGAACT CCGAAAAGTG 
ACGGATGACA AAAACATGCT CATGAATCAA ATGGGCATTC GTGCTGGTGT ATGTCTTCAC 
AATGGAATTG TGCACAAAAA CAAGGAGGAA TGGACAGTGG ACGACTGCAC AGAGTGCACT 
TGCCAGAATT CTGCAACGGT GTGCCGCAAG ATTTCATGTC CCCTAATCCC ATGTGCAAAT 
GCCACAGTAC CTGATGGAGA GTGCTGCCCT CGCTGTGGAA CACCGAGCGA CTCCGCCGAG 
GATGGTTGGT CCCCCTGGTC TGAATGGACC CATTGTTCAG TGTCCTGTGG AAGGGGCATT 
CAGCAGCGCG GCCGTTCCTG CGACCGCATC AATAACAACT GTGAGGGCAC GTCAGTGCAG 
ACCAGAGACT GCTACCTCCA GGAGTGCGAC AAGCGCTTTA AGCAAGACGG TAGCTGGAGC 
CACTGGTCAC CCTGGTCATC GTGTTCGGTC ACATGTGGTG CTGGTGTTAT TACACGCATC 
CGTCTCTGTA ACTCCCCGAC ACCTCAAATG GATGGCAAAG ACTGCCAGGG TGAAGGGCGG 
CAAACTGAGA GGTGTGAGAA ATCACCATGT CCGATCAACG GTGGCTGGGG ACCATGGTCG 
CCCTGGGATA CTTGCTCTGT TACCTGTGGT GGTGGCGTCC AAAACCGTAA GCGTCTTTGC 
AATAATCCTG TACCCAAACA TGGTGGCAAA GAGTGTGTGG GAGATGCAAA GGTCAGCCAG 
ATTTGCAACA AACAAGCCTG TCCTGTTGAT GGATGTCTTT CGAGTCCGTG CTTTGAAGGG 
GCCCAGTGCA CAAGCTTTCC AGATGGGTCC TGGAAATGTG GAAAATGCCC CACAGGATAC 
ACTGGCAATG GAATCAACTG CAAAGATGTC AATGAGTGTA AGGAAGTACC AGATGCCTGC 
TTTGAATTTA ATGGAGTCCA CAGATGTGAG AACACAGTGC CAGGCTACAA CTGTCTCCCT 
TGCCCCACTC GTTACACCGG GCCACAGCCG TTTGGCCGTG GAGTGGAGGA CGCCGCTGCT 
AAGAAGCAGG TGTGCACTCC TCGAAATCCC TGCCTGGATG GAAGCCACGA CTGCAATAAA 
AACGCTCGCT GCAACTACCT GGGCCATTTC TCTGACCCCA TGTTCCGCTG TGAATGCAAA 
CCTGGCTTTG CTGGAAATGG ACACATCTGT GGAGAAGATA CTGATCTTGA TGGTTGGCCG 
AATGCTGATC TCGTGTGTGT TGAGAACGCA ACCTATCACT GCAAGAAGGA CAACTGCCCA 
AATCTTCCCA ACTCAGGGCA AGAAGACTAT GACAAGGATG GCATTGGTGA TGCATGTGAC 
GATGATGATG ATGACGATGG CATTCCTGAT GACAGGGATA ACTGCCCACT CGTCTTCAAC 
CCGAGACAGT ACGACTATGA TCGGGATGAT GTTGGTGACC GTTGTGATAA CTGTCCCTAC 
AACAGCAACC CAGACCAGAC AGACACTGAC AACAATGGAG AAGGAGACGC CTGTGCTGTG 
GACATTGATG GAGATGGTAT TCTGAACGAG AAAGACAACT GTCCGTATCT GTACAACGTC 
GACCAGAAAG ACACTGATCT TGATGGAGTT GGAGACCAGT GTGACAACTG TCCATTGGAA 
CACAACCCAG ATCAGCTGGA CTCTGATTCT GACCGCGTTG GAGACAAATG TGACAGTAAC 
CAGGATATCG ATGAAGACGG TCACCAGAAC AACCTGGACA ACTGTCCATA CATCCCCAAT 
GCCAACCAGG CTGATCATGA CAAGGACGGT AAAGGAGATG CCTGCGACTA CGATGATGAT 
AATGATGGCA TTCCTGACGA AAAAGACAAC TGCAGACTGG TCTTCAATCC AGATCAGCTA 
GATTCTGATG GCGATGGACG TGGCGATGCC TGTAAAGATG ACTTTGACAT GGACAATGTT 
CTGGATATCT ATGATGTCTG TCCTGAGAAC TTCGATATCA GTGAAACCGA CTTCCGCAAG 
TTCCAGATGG TTCCTCTGGA TCCCAAGGGC ACTTCTCAGA TCGATCCCAA CTGGGTGGTT 
CGTCATCAAG GAAAAGAGCT GGTTCAGACA GTCAACTGCG ACCCTGGCAT TGCTGTTGGT 
TTTGATGAGT TCAATTCAGT GGACTTCAGT GGAACATTCT TCATCAACAC TGAGAGGGAT 
GACGACTATG CTGGTTTTGT TTTCGGATAC CAGTCCAGCT CTCGCTTCTA TGTGGTCATG 
TGGAAGCAGA TCACACAGAC CTACTGGTCC AGCACACCTA CCAAAGCACA GGGTTACTCA 
GGGCTGTCCA TCAAAGTGGT TAATTCCACC ACAGGACCTG GGGAGCATCT GAGAAACGCC 
CTTTGGCACA CTGGAGACAC ACCAGGACAG GTGCGCACAT TGTGGCATGA CCCAAAGAAT 
GTGGGATGGA AGGACTTTAC TGCTTACAGA TGGCACCTGA CACACAGACC AAGAACTGGA 
CACATAAGAG TGGTCATGTA TGAAGGCAAA AAGATCATGG CAGATTCTGG CAGAATCTAC 
GACAAAACAT ACGCAGGCGG AAGACTAGGT CTCTTCGTCT TCTCACAAGA GATGGTGTAT 
TTCTCAGACC TCAAATACGA ATGCAGAGAT GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1B ( XM_685303.9 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_690395.4 Danio rerio thrombospondin 1b (thbs1b), transcript variant X2, mRNA.

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CloneID	ODa25040
Clone ID Related Accession (Same CDS sequence)	XM_685303.9 , XM_685303.8
Accession Version	XM_685303.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	thrombospondin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_685303.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG 
AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC 
CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC 
ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG 
GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC 
AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG 
TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA 
GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC 
TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA 
GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC 
ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAATGACTGA TATCATTACC 
CTTGACAATC CCATAAATGG ATCCAGCCCT GCAATCAGGA CAGATTACAC TGGCCACAAA 
ACAAAAGATC TGCAAATGAT TTGTGGCTTT TCATGTGAGG ATCTGGCTGC CATGTTTAAG 
GAACTGAAAG GTCTTGGTGT GGTGGTTCAA GAACTGTCCA ATGAACTCCG AAAAGTGACG 
GATGACAAAA ACATGCTCAT GAATCAAATG GGCATTCGTG CTGGTGTATG TCTTCACAAT 
GGAATTGTGC ACAAAAACAA GGAGGAATGG ACAGTGGACG ACTGCACAGA GTGCACTTGC 
CAGAATTCTG CAACGGTGTG CCGCAAGATT TCATGTCCCC TAATCCCATG TGCAAATGCC 
ACAGTACCTG ATGGAGAGTG CTGCCCTCGC TGTGGAACAC CGAGCGACTC CGCCGAGGAT 
GGTTGGTCCC CCTGGTCTGA ATGGACCCAT TGTTCAGTGT CCTGTGGAAG GGGCATTCAG 
CAGCGCGGCC GTTCCTGCGA CCGCATCAAT AACAACTGTG AGGGCACGTC AGTGCAGACC 
AGAGACTGCT ACCTCCAGGA GTGCGACAAG CGCTTTAAGC AAGACGGTAG CTGGAGCCAC 
TGGTCACCCT GGTCATCGTG TTCGGTCACA TGTGGTGCTG GTGTTATTAC ACGCATCCGT 
CTCTGTAACT CCCCGACACC TCAAATGGAT GGCAAAGACT GCCAGGGTGA AGGGCGGCAA 
ACTGAGAGGT GTGAGAAATC ACCATGTCCG ATCAACGGTG GCTGGGGACC ATGGTCGCCC 
TGGGATACTT GCTCTGTTAC CTGTGGTGGT GGCGTCCAAA ACCGTAAGCG TCTTTGCAAT 
AATCCTGTAC CCAAACATGG TGGCAAAGAG TGTGTGGGAG ATGCAAAGGT CAGCCAGATT 
TGCAACAAAC AAGCCTGTCC TGTTGATGGA TGTCTTTCGA GTCCGTGCTT TGAAGGGGCC 
CAGTGCACAA GCTTTCCAGA TGGGTCCTGG AAATGTGGAA AATGCCCCAC AGGATACACT 
GGCAATGGAA TCAACTGCAA AGATGTCAAT GAGTGTAAGG AAGTACCAGA TGCCTGCTTT 
GAATTTAATG GAGTCCACAG ATGTGAGAAC ACAGTGCCAG GCTACAACTG TCTCCCTTGC 
CCCACTCGTT ACACCGGGCC ACAGCCGTTT GGCCGTGGAG TGGAGGACGC CGCTGCTAAG 
AAGCAGGTGT GCACTCCTCG AAATCCCTGC CTGGATGGAA GCCACGACTG CAATAAAAAC 
GCTCGCTGCA ACTACCTGGG CCATTTCTCT GACCCCATGT TCCGCTGTGA ATGCAAACCT 
GGCTTTGCTG GAAATGGACA CATCTGTGGA GAAGATACTG ATCTTGATGG TTGGCCGAAT 
GCTGATCTCG TGTGTGTTGA GAACGCAACC TATCACTGCA AGAAGGACAA CTGCCCAAAT 
CTTCCCAACT CAGGGCAAGA AGACTATGAC AAGGATGGCA TTGGTGATGC ATGTGACGAT 
GATGATGATG ACGATGGCAT TCCTGATGAC AGGGATAACT GCCCACTCGT CTTCAACCCG 
AGACAGTACG ACTATGATCG GGATGATGTT GGTGACCGTT GTGATAACTG TCCCTACAAC 
AGCAACCCAG ACCAGACAGA CACTGACAAC AATGGAGAAG GAGACGCCTG TGCTGTGGAC 
ATTGATGGAG ATGGTATTCT GAACGAGAAA GACAACTGTC CGTATCTGTA CAACGTCGAC 
CAGAAAGACA CTGATCTTGA TGGAGTTGGA GACCAGTGTG ACAACTGTCC ATTGGAACAC 
AACCCAGATC AGCTGGACTC TGATTCTGAC CGCGTTGGAG ACAAATGTGA CAGTAACCAG 
GATATCGATG AAGACGGTCA CCAGAACAAC CTGGACAACT GTCCATACAT CCCCAATGCC 
AACCAGGCTG ATCATGACAA GGACGGTAAA GGAGATGCCT GCGACTACGA TGATGATAAT 
GATGGCATTC CTGACGAAAA AGACAACTGC AGACTGGTCT TCAATCCAGA TCAGCTAGAT 
TCTGATGGCG ATGGACGTGG CGATGCCTGT AAAGATGACT TTGACATGGA CAATGTTCTG 
GATATCTATG ATGTCTGTCC TGAGAACTTC GATATCAGTG AAACCGACTT CCGCAAGTTC 
CAGATGGTTC CTCTGGATCC CAAGGGCACT TCTCAGATCG ATCCCAACTG GGTGGTTCGT 
CATCAAGGAA AAGAGCTGGT TCAGACAGTC AACTGCGACC CTGGCATTGC TGTTGGTTTT 
GATGAGTTCA ATTCAGTGGA CTTCAGTGGA ACATTCTTCA TCAACACTGA GAGGGATGAC 
GACTATGCTG GTTTTGTTTT CGGATACCAG TCCAGCTCTC GCTTCTATGT GGTCATGTGG 
AAGCAGATCA CACAGACCTA CTGGTCCAGC ACACCTACCA AAGCACAGGG TTACTCAGGG 
CTGTCCATCA AAGTGGTTAA TTCCACCACA GGACCTGGGG AGCATCTGAG AAACGCCCTT 
TGGCACACTG GAGACACACC AGGACAGGTG CGCACATTGT GGCATGACCC AAAGAATGTG 
GGATGGAAGG ACTTTACTGC TTACAGATGG CACCTGACAC ACAGACCAAG AACTGGACAC 
ATAAGAGTGG TCATGTATGA AGGCAAAAAG ATCATGGCAG ATTCTGGCAG AATCTACGAC 
AAAACATACG CAGGCGGAAG ACTAGGTCTC TTCGTCTTCT CACAAGAGAT GGTGTATTTC 
TCAGACCTCA AATACGAATG CAGAGATGCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_690395.4
CDS	187..3699
Translation	MKSTAIFLLLMLWNCESARVAESRDDNSVYDLFELVQVPRKNHGVTLVKGDDPYSPAYKILNPDLIPPVPESAFRDLIDSIHAEKGFLLLVNFKQFKRTRGSLLTVEKNDGSGPVFEIVSNGKANTLDIVFSTENKQQVVSIEEADLAVGHWKNITLFVQEDRVQFYVGCEEVNVAELDASIHTILTQEIPGVAKMRIGKGAVKDRFMGVLQNVRFVFGTTLEAILRNKGCQNSMTDIITLDNPINGSSPAIRTDYTGHKTKDLQMICGFSCEDLAAMFKELKGLGVVVQELSNELRKVTDDKNMLMNQMGIRAGVCLHNGIVHKNKEEWTVDDCTECTCQNSATVCRKISCPLIPCANATVPDGECCPRCGTPSDSAEDGWSPWSEWTHCSVSCGRGIQQRGRSCDRINNNCEGTSVQTRDCYLQECDKRFKQDGSWSHWSPWSSCSVTCGAGVITRIRLCNSPTPQMDGKDCQGEGRQTERCEKSPCPINGGWGPWSPWDTCSVTCGGGVQNRKRLCNNPVPKHGGKECVGDAKVSQICNKQACPVDGCLSSPCFEGAQCTSFPDGSWKCGKCPTGYTGNGINCKDVNECKEVPDACFEFNGVHRCENTVPGYNCLPCPTRYTGPQPFGRGVEDAAAKKQVCTPRNPCLDGSHDCNKNARCNYLGHFSDPMFRCECKPGFAGNGHICGEDTDLDGWPNADLVCVENATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDDDGIPDDRDNCPLVFNPRQYDYDRDDVGDRCDNCPYNSNPDQTDTDNNGEGDACAVDIDGDGILNEKDNCPYLYNVDQKDTDLDGVGDQCDNCPLEHNPDQLDSDSDRVGDKCDSNQDIDEDGHQNNLDNCPYIPNANQADHDKDGKGDACDYDDDNDGIPDEKDNCRLVFNPDQLDSDGDGRGDACKDDFDMDNVLDIYDVCPENFDISETDFRKFQMVPLDPKGTSQIDPNWVVRHQGKELVQTVNCDPGIAVGFDEFNSVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQITQTYWSSTPTKAQGYSGLSIKVVNSTTGPGEHLRNALWHTGDTPGQVRTLWHDPKNVGWKDFTAYRWHLTHRPRTGHIRVVMYEGKKIMADSGRIYDKTYAGGRLGLFVFSQEMVYFSDLKYECRDA

Target ORF information:

RefSeq Version	XM_685303.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin 1b (thbs1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_685303.9

ORF Insert Sequence:

ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG 
AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC 
CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC 
ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG 
GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC 
AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG 
TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA 
GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC 
TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA 
GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC 
ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAATGACTGA TATCATTACC 
CTTGACAATC CCATAAATGG ATCCAGCCCT GCAATCAGGA CAGATTACAC TGGCCACAAA 
ACAAAAGATC TGCAAATGAT TTGTGGCTTT TCATGTGAGG ATCTGGCTGC CATGTTTAAG 
GAACTGAAAG GTCTTGGTGT GGTGGTTCAA GAACTGTCCA ATGAACTCCG AAAAGTGACG 
GATGACAAAA ACATGCTCAT GAATCAAATG GGCATTCGTG CTGGTGTATG TCTTCACAAT 
GGAATTGTGC ACAAAAACAA GGAGGAATGG ACAGTGGACG ACTGCACAGA GTGCACTTGC 
CAGAATTCTG CAACGGTGTG CCGCAAGATT TCATGTCCCC TAATCCCATG TGCAAATGCC 
ACAGTACCTG ATGGAGAGTG CTGCCCTCGC TGTGGAACAC CGAGCGACTC CGCCGAGGAT 
GGTTGGTCCC CCTGGTCTGA ATGGACCCAT TGTTCAGTGT CCTGTGGAAG GGGCATTCAG 
CAGCGCGGCC GTTCCTGCGA CCGCATCAAT AACAACTGTG AGGGCACGTC AGTGCAGACC 
AGAGACTGCT ACCTCCAGGA GTGCGACAAG CGCTTTAAGC AAGACGGTAG CTGGAGCCAC 
TGGTCACCCT GGTCATCGTG TTCGGTCACA TGTGGTGCTG GTGTTATTAC ACGCATCCGT 
CTCTGTAACT CCCCGACACC TCAAATGGAT GGCAAAGACT GCCAGGGTGA AGGGCGGCAA 
ACTGAGAGGT GTGAGAAATC ACCATGTCCG ATCAACGGTG GCTGGGGACC ATGGTCGCCC 
TGGGATACTT GCTCTGTTAC CTGTGGTGGT GGCGTCCAAA ACCGTAAGCG TCTTTGCAAT 
AATCCTGTAC CCAAACATGG TGGCAAAGAG TGTGTGGGAG ATGCAAAGGT CAGCCAGATT 
TGCAACAAAC AAGCCTGTCC TGTTGATGGA TGTCTTTCGA GTCCGTGCTT TGAAGGGGCC 
CAGTGCACAA GCTTTCCAGA TGGGTCCTGG AAATGTGGAA AATGCCCCAC AGGATACACT 
GGCAATGGAA TCAACTGCAA AGATGTCAAT GAGTGTAAGG AAGTACCAGA TGCCTGCTTT 
GAATTTAATG GAGTCCACAG ATGTGAGAAC ACAGTGCCAG GCTACAACTG TCTCCCTTGC 
CCCACTCGTT ACACCGGGCC ACAGCCGTTT GGCCGTGGAG TGGAGGACGC CGCTGCTAAG 
AAGCAGGTGT GCACTCCTCG AAATCCCTGC CTGGATGGAA GCCACGACTG CAATAAAAAC 
GCTCGCTGCA ACTACCTGGG CCATTTCTCT GACCCCATGT TCCGCTGTGA ATGCAAACCT 
GGCTTTGCTG GAAATGGACA CATCTGTGGA GAAGATACTG ATCTTGATGG TTGGCCGAAT 
GCTGATCTCG TGTGTGTTGA GAACGCAACC TATCACTGCA AGAAGGACAA CTGCCCAAAT 
CTTCCCAACT CAGGGCAAGA AGACTATGAC AAGGATGGCA TTGGTGATGC ATGTGACGAT 
GATGATGATG ACGATGGCAT TCCTGATGAC AGGGATAACT GCCCACTCGT CTTCAACCCG 
AGACAGTACG ACTATGATCG GGATGATGTT GGTGACCGTT GTGATAACTG TCCCTACAAC 
AGCAACCCAG ACCAGACAGA CACTGACAAC AATGGAGAAG GAGACGCCTG TGCTGTGGAC 
ATTGATGGAG ATGGTATTCT GAACGAGAAA GACAACTGTC CGTATCTGTA CAACGTCGAC 
CAGAAAGACA CTGATCTTGA TGGAGTTGGA GACCAGTGTG ACAACTGTCC ATTGGAACAC 
AACCCAGATC AGCTGGACTC TGATTCTGAC CGCGTTGGAG ACAAATGTGA CAGTAACCAG 
GATATCGATG AAGACGGTCA CCAGAACAAC CTGGACAACT GTCCATACAT CCCCAATGCC 
AACCAGGCTG ATCATGACAA GGACGGTAAA GGAGATGCCT GCGACTACGA TGATGATAAT 
GATGGCATTC CTGACGAAAA AGACAACTGC AGACTGGTCT TCAATCCAGA TCAGCTAGAT 
TCTGATGGCG ATGGACGTGG CGATGCCTGT AAAGATGACT TTGACATGGA CAATGTTCTG 
GATATCTATG ATGTCTGTCC TGAGAACTTC GATATCAGTG AAACCGACTT CCGCAAGTTC 
CAGATGGTTC CTCTGGATCC CAAGGGCACT TCTCAGATCG ATCCCAACTG GGTGGTTCGT 
CATCAAGGAA AAGAGCTGGT TCAGACAGTC AACTGCGACC CTGGCATTGC TGTTGGTTTT 
GATGAGTTCA ATTCAGTGGA CTTCAGTGGA ACATTCTTCA TCAACACTGA GAGGGATGAC 
GACTATGCTG GTTTTGTTTT CGGATACCAG TCCAGCTCTC GCTTCTATGT GGTCATGTGG 
AAGCAGATCA CACAGACCTA CTGGTCCAGC ACACCTACCA AAGCACAGGG TTACTCAGGG 
CTGTCCATCA AAGTGGTTAA TTCCACCACA GGACCTGGGG AGCATCTGAG AAACGCCCTT 
TGGCACACTG GAGACACACC AGGACAGGTG CGCACATTGT GGCATGACCC AAAGAATGTG 
GGATGGAAGG ACTTTACTGC TTACAGATGG CACCTGACAC ACAGACCAAG AACTGGACAC 
ATAAGAGTGG TCATGTATGA AGGCAAAAAG ATCATGGCAG ATTCTGGCAG AATCTACGAC 
AAAACATACG CAGGCGGAAG ACTAGGTCTC TTCGTCTTCT CACAAGAGAT GGTGTATTTC 
TCAGACCTCA AATACGAATG CAGAGATGCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1B ( XM_685303.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_690395.4 Danio rerio thrombospondin 1b (thbs1b), transcript variant X2, mRNA.

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CloneID	ODa25040
Clone ID Related Accession (Same CDS sequence)	XM_685303.9 , XM_685303.8
Accession Version	XM_685303.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	thrombospondin-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_685303.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG 
AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC 
CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC 
ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG 
GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC 
AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG 
TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA 
GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC 
TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA 
GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC 
ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAATGACTGA TATCATTACC 
CTTGACAATC CCATAAATGG ATCCAGCCCT GCAATCAGGA CAGATTACAC TGGCCACAAA 
ACAAAAGATC TGCAAATGAT TTGTGGCTTT TCATGTGAGG ATCTGGCTGC CATGTTTAAG 
GAACTGAAAG GTCTTGGTGT GGTGGTTCAA GAACTGTCCA ATGAACTCCG AAAAGTGACG 
GATGACAAAA ACATGCTCAT GAATCAAATG GGCATTCGTG CTGGTGTATG TCTTCACAAT 
GGAATTGTGC ACAAAAACAA GGAGGAATGG ACAGTGGACG ACTGCACAGA GTGCACTTGC 
CAGAATTCTG CAACGGTGTG CCGCAAGATT TCATGTCCCC TAATCCCATG TGCAAATGCC 
ACAGTACCTG ATGGAGAGTG CTGCCCTCGC TGTGGAACAC CGAGCGACTC CGCCGAGGAT 
GGTTGGTCCC CCTGGTCTGA ATGGACCCAT TGTTCAGTGT CCTGTGGAAG GGGCATTCAG 
CAGCGCGGCC GTTCCTGCGA CCGCATCAAT AACAACTGTG AGGGCACGTC AGTGCAGACC 
AGAGACTGCT ACCTCCAGGA GTGCGACAAG CGCTTTAAGC AAGACGGTAG CTGGAGCCAC 
TGGTCACCCT GGTCATCGTG TTCGGTCACA TGTGGTGCTG GTGTTATTAC ACGCATCCGT 
CTCTGTAACT CCCCGACACC TCAAATGGAT GGCAAAGACT GCCAGGGTGA AGGGCGGCAA 
ACTGAGAGGT GTGAGAAATC ACCATGTCCG ATCAACGGTG GCTGGGGACC ATGGTCGCCC 
TGGGATACTT GCTCTGTTAC CTGTGGTGGT GGCGTCCAAA ACCGTAAGCG TCTTTGCAAT 
AATCCTGTAC CCAAACATGG TGGCAAAGAG TGTGTGGGAG ATGCAAAGGT CAGCCAGATT 
TGCAACAAAC AAGCCTGTCC TGTTGATGGA TGTCTTTCGA GTCCGTGCTT TGAAGGGGCC 
CAGTGCACAA GCTTTCCAGA TGGGTCCTGG AAATGTGGAA AATGCCCCAC AGGATACACT 
GGCAATGGAA TCAACTGCAA AGATGTCAAT GAGTGTAAGG AAGTACCAGA TGCCTGCTTT 
GAATTTAATG GAGTCCACAG ATGTGAGAAC ACAGTGCCAG GCTACAACTG TCTCCCTTGC 
CCCACTCGTT ACACCGGGCC ACAGCCGTTT GGCCGTGGAG TGGAGGACGC CGCTGCTAAG 
AAGCAGGTGT GCACTCCTCG AAATCCCTGC CTGGATGGAA GCCACGACTG CAATAAAAAC 
GCTCGCTGCA ACTACCTGGG CCATTTCTCT GACCCCATGT TCCGCTGTGA ATGCAAACCT 
GGCTTTGCTG GAAATGGACA CATCTGTGGA GAAGATACTG ATCTTGATGG TTGGCCGAAT 
GCTGATCTCG TGTGTGTTGA GAACGCAACC TATCACTGCA AGAAGGACAA CTGCCCAAAT 
CTTCCCAACT CAGGGCAAGA AGACTATGAC AAGGATGGCA TTGGTGATGC ATGTGACGAT 
GATGATGATG ACGATGGCAT TCCTGATGAC AGGGATAACT GCCCACTCGT CTTCAACCCG 
AGACAGTACG ACTATGATCG GGATGATGTT GGTGACCGTT GTGATAACTG TCCCTACAAC 
AGCAACCCAG ACCAGACAGA CACTGACAAC AATGGAGAAG GAGACGCCTG TGCTGTGGAC 
ATTGATGGAG ATGGTATTCT GAACGAGAAA GACAACTGTC CGTATCTGTA CAACGTCGAC 
CAGAAAGACA CTGATCTTGA TGGAGTTGGA GACCAGTGTG ACAACTGTCC ATTGGAACAC 
AACCCAGATC AGCTGGACTC TGATTCTGAC CGCGTTGGAG ACAAATGTGA CAGTAACCAG 
GATATCGATG AAGACGGTCA CCAGAACAAC CTGGACAACT GTCCATACAT CCCCAATGCC 
AACCAGGCTG ATCATGACAA GGACGGTAAA GGAGATGCCT GCGACTACGA TGATGATAAT 
GATGGCATTC CTGACGAAAA AGACAACTGC AGACTGGTCT TCAATCCAGA TCAGCTAGAT 
TCTGATGGCG ATGGACGTGG CGATGCCTGT AAAGATGACT TTGACATGGA CAATGTTCTG 
GATATCTATG ATGTCTGTCC TGAGAACTTC GATATCAGTG AAACCGACTT CCGCAAGTTC 
CAGATGGTTC CTCTGGATCC CAAGGGCACT TCTCAGATCG ATCCCAACTG GGTGGTTCGT 
CATCAAGGAA AAGAGCTGGT TCAGACAGTC AACTGCGACC CTGGCATTGC TGTTGGTTTT 
GATGAGTTCA ATTCAGTGGA CTTCAGTGGA ACATTCTTCA TCAACACTGA GAGGGATGAC 
GACTATGCTG GTTTTGTTTT CGGATACCAG TCCAGCTCTC GCTTCTATGT GGTCATGTGG 
AAGCAGATCA CACAGACCTA CTGGTCCAGC ACACCTACCA AAGCACAGGG TTACTCAGGG 
CTGTCCATCA AAGTGGTTAA TTCCACCACA GGACCTGGGG AGCATCTGAG AAACGCCCTT 
TGGCACACTG GAGACACACC AGGACAGGTG CGCACATTGT GGCATGACCC AAAGAATGTG 
GGATGGAAGG ACTTTACTGC TTACAGATGG CACCTGACAC ACAGACCAAG AACTGGACAC 
ATAAGAGTGG TCATGTATGA AGGCAAAAAG ATCATGGCAG ATTCTGGCAG AATCTACGAC 
AAAACATACG CAGGCGGAAG ACTAGGTCTC TTCGTCTTCT CACAAGAGAT GGTGTATTTC 
TCAGACCTCA AATACGAATG CAGAGATGCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_690395.4
CDS	191..3703
Translation	MKSTAIFLLLMLWNCESARVAESRDDNSVYDLFELVQVPRKNHGVTLVKGDDPYSPAYKILNPDLIPPVPESAFRDLIDSIHAEKGFLLLVNFKQFKRTRGSLLTVEKNDGSGPVFEIVSNGKANTLDIVFSTENKQQVVSIEEADLAVGHWKNITLFVQEDRVQFYVGCEEVNVAELDASIHTILTQEIPGVAKMRIGKGAVKDRFMGVLQNVRFVFGTTLEAILRNKGCQNSMTDIITLDNPINGSSPAIRTDYTGHKTKDLQMICGFSCEDLAAMFKELKGLGVVVQELSNELRKVTDDKNMLMNQMGIRAGVCLHNGIVHKNKEEWTVDDCTECTCQNSATVCRKISCPLIPCANATVPDGECCPRCGTPSDSAEDGWSPWSEWTHCSVSCGRGIQQRGRSCDRINNNCEGTSVQTRDCYLQECDKRFKQDGSWSHWSPWSSCSVTCGAGVITRIRLCNSPTPQMDGKDCQGEGRQTERCEKSPCPINGGWGPWSPWDTCSVTCGGGVQNRKRLCNNPVPKHGGKECVGDAKVSQICNKQACPVDGCLSSPCFEGAQCTSFPDGSWKCGKCPTGYTGNGINCKDVNECKEVPDACFEFNGVHRCENTVPGYNCLPCPTRYTGPQPFGRGVEDAAAKKQVCTPRNPCLDGSHDCNKNARCNYLGHFSDPMFRCECKPGFAGNGHICGEDTDLDGWPNADLVCVENATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDDDGIPDDRDNCPLVFNPRQYDYDRDDVGDRCDNCPYNSNPDQTDTDNNGEGDACAVDIDGDGILNEKDNCPYLYNVDQKDTDLDGVGDQCDNCPLEHNPDQLDSDSDRVGDKCDSNQDIDEDGHQNNLDNCPYIPNANQADHDKDGKGDACDYDDDNDGIPDEKDNCRLVFNPDQLDSDGDGRGDACKDDFDMDNVLDIYDVCPENFDISETDFRKFQMVPLDPKGTSQIDPNWVVRHQGKELVQTVNCDPGIAVGFDEFNSVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQITQTYWSSTPTKAQGYSGLSIKVVNSTTGPGEHLRNALWHTGDTPGQVRTLWHDPKNVGWKDFTAYRWHLTHRPRTGHIRVVMYEGKKIMADSGRIYDKTYAGGRLGLFVFSQEMVYFSDLKYECRDA

Target ORF information:

RefSeq Version	XM_685303.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio thrombospondin 1b (thbs1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_685303.8

ORF Insert Sequence:

ATGAAGTCAA CTGCAATCTT TTTGCTACTG ATGCTTTGGA ACTGCGAGAG CGCGAGAGTC 
GCAGAGAGTC GAGACGACAA CAGTGTTTAC GATCTGTTCG AGCTGGTCCA AGTACCAAGG 
AAAAACCACG GAGTGACCCT GGTGAAAGGC GACGACCCGT ACAGCCCCGC CTACAAGATC 
CTGAACCCCG ACCTGATCCC CCCGGTCCCC GAGAGCGCCT TTAGGGACCT AATCGATTCC 
ATTCACGCCG AGAAAGGATT TCTGCTGCTG GTTAATTTCA AGCAGTTCAA ACGCACGAGG 
GGAAGCCTGC TGACTGTGGA GAAGAATGAC GGTTCTGGAC CCGTGTTTGA AATCGTTTCC 
AACGGGAAAG CCAACACTCT GGACATTGTT TTTTCCACCG AGAACAAGCA GCAGGTCGTG 
TCCATCGAGG AAGCGGACTT GGCGGTGGGA CACTGGAAAA ACATCACTCT TTTCGTGCAA 
GAGGACAGGG TTCAGTTCTA TGTGGGATGT GAGGAGGTGA ACGTGGCGGA GCTCGATGCC 
TCAATCCACA CCATCCTGAC TCAGGAGATT CCAGGTGTGG CCAAAATGAG GATCGGTAAA 
GGCGCGGTGA AGGACAGGTT CATGGGGGTG TTGCAAAACG TGCGCTTTGT ATTCGGAACC 
ACGCTGGAAG CCATCCTGAG GAATAAAGGG TGCCAAAACT CAATGACTGA TATCATTACC 
CTTGACAATC CCATAAATGG ATCCAGCCCT GCAATCAGGA CAGATTACAC TGGCCACAAA 
ACAAAAGATC TGCAAATGAT TTGTGGCTTT TCATGTGAGG ATCTGGCTGC CATGTTTAAG 
GAACTGAAAG GTCTTGGTGT GGTGGTTCAA GAACTGTCCA ATGAACTCCG AAAAGTGACG 
GATGACAAAA ACATGCTCAT GAATCAAATG GGCATTCGTG CTGGTGTATG TCTTCACAAT 
GGAATTGTGC ACAAAAACAA GGAGGAATGG ACAGTGGACG ACTGCACAGA GTGCACTTGC 
CAGAATTCTG CAACGGTGTG CCGCAAGATT TCATGTCCCC TAATCCCATG TGCAAATGCC 
ACAGTACCTG ATGGAGAGTG CTGCCCTCGC TGTGGAACAC CGAGCGACTC CGCCGAGGAT 
GGTTGGTCCC CCTGGTCTGA ATGGACCCAT TGTTCAGTGT CCTGTGGAAG GGGCATTCAG 
CAGCGCGGCC GTTCCTGCGA CCGCATCAAT AACAACTGTG AGGGCACGTC AGTGCAGACC 
AGAGACTGCT ACCTCCAGGA GTGCGACAAG CGCTTTAAGC AAGACGGTAG CTGGAGCCAC 
TGGTCACCCT GGTCATCGTG TTCGGTCACA TGTGGTGCTG GTGTTATTAC ACGCATCCGT 
CTCTGTAACT CCCCGACACC TCAAATGGAT GGCAAAGACT GCCAGGGTGA AGGGCGGCAA 
ACTGAGAGGT GTGAGAAATC ACCATGTCCG ATCAACGGTG GCTGGGGACC ATGGTCGCCC 
TGGGATACTT GCTCTGTTAC CTGTGGTGGT GGCGTCCAAA ACCGTAAGCG TCTTTGCAAT 
AATCCTGTAC CCAAACATGG TGGCAAAGAG TGTGTGGGAG ATGCAAAGGT CAGCCAGATT 
TGCAACAAAC AAGCCTGTCC TGTTGATGGA TGTCTTTCGA GTCCGTGCTT TGAAGGGGCC 
CAGTGCACAA GCTTTCCAGA TGGGTCCTGG AAATGTGGAA AATGCCCCAC AGGATACACT 
GGCAATGGAA TCAACTGCAA AGATGTCAAT GAGTGTAAGG AAGTACCAGA TGCCTGCTTT 
GAATTTAATG GAGTCCACAG ATGTGAGAAC ACAGTGCCAG GCTACAACTG TCTCCCTTGC 
CCCACTCGTT ACACCGGGCC ACAGCCGTTT GGCCGTGGAG TGGAGGACGC CGCTGCTAAG 
AAGCAGGTGT GCACTCCTCG AAATCCCTGC CTGGATGGAA GCCACGACTG CAATAAAAAC 
GCTCGCTGCA ACTACCTGGG CCATTTCTCT GACCCCATGT TCCGCTGTGA ATGCAAACCT 
GGCTTTGCTG GAAATGGACA CATCTGTGGA GAAGATACTG ATCTTGATGG TTGGCCGAAT 
GCTGATCTCG TGTGTGTTGA GAACGCAACC TATCACTGCA AGAAGGACAA CTGCCCAAAT 
CTTCCCAACT CAGGGCAAGA AGACTATGAC AAGGATGGCA TTGGTGATGC ATGTGACGAT 
GATGATGATG ACGATGGCAT TCCTGATGAC AGGGATAACT GCCCACTCGT CTTCAACCCG 
AGACAGTACG ACTATGATCG GGATGATGTT GGTGACCGTT GTGATAACTG TCCCTACAAC 
AGCAACCCAG ACCAGACAGA CACTGACAAC AATGGAGAAG GAGACGCCTG TGCTGTGGAC 
ATTGATGGAG ATGGTATTCT GAACGAGAAA GACAACTGTC CGTATCTGTA CAACGTCGAC 
CAGAAAGACA CTGATCTTGA TGGAGTTGGA GACCAGTGTG ACAACTGTCC ATTGGAACAC 
AACCCAGATC AGCTGGACTC TGATTCTGAC CGCGTTGGAG ACAAATGTGA CAGTAACCAG 
GATATCGATG AAGACGGTCA CCAGAACAAC CTGGACAACT GTCCATACAT CCCCAATGCC 
AACCAGGCTG ATCATGACAA GGACGGTAAA GGAGATGCCT GCGACTACGA TGATGATAAT 
GATGGCATTC CTGACGAAAA AGACAACTGC AGACTGGTCT TCAATCCAGA TCAGCTAGAT 
TCTGATGGCG ATGGACGTGG CGATGCCTGT AAAGATGACT TTGACATGGA CAATGTTCTG 
GATATCTATG ATGTCTGTCC TGAGAACTTC GATATCAGTG AAACCGACTT CCGCAAGTTC 
CAGATGGTTC CTCTGGATCC CAAGGGCACT TCTCAGATCG ATCCCAACTG GGTGGTTCGT 
CATCAAGGAA AAGAGCTGGT TCAGACAGTC AACTGCGACC CTGGCATTGC TGTTGGTTTT 
GATGAGTTCA ATTCAGTGGA CTTCAGTGGA ACATTCTTCA TCAACACTGA GAGGGATGAC 
GACTATGCTG GTTTTGTTTT CGGATACCAG TCCAGCTCTC GCTTCTATGT GGTCATGTGG 
AAGCAGATCA CACAGACCTA CTGGTCCAGC ACACCTACCA AAGCACAGGG TTACTCAGGG 
CTGTCCATCA AAGTGGTTAA TTCCACCACA GGACCTGGGG AGCATCTGAG AAACGCCCTT 
TGGCACACTG GAGACACACC AGGACAGGTG CGCACATTGT GGCATGACCC AAAGAATGTG 
GGATGGAAGG ACTTTACTGC TTACAGATGG CACCTGACAC ACAGACCAAG AACTGGACAC 
ATAAGAGTGG TCATGTATGA AGGCAAAAAG ATCATGGCAG ATTCTGGCAG AATCTACGAC 
AAAACATACG CAGGCGGAAG ACTAGGTCTC TTCGTCTTCT CACAAGAGAT GGTGTATTTC 
TCAGACCTCA AATACGAATG CAGAGATGCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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THBS1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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