atp2b1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	atp2b1b
Entrez Gene ID	561190
Full Name	ATPase plasma membrane Ca2+ transporting 1b
General protein information	Preferred Names ATPase plasma membrane Ca2+ transporting 1b Names plasma membrane calcium-transporting ATPase 3 ATPase, Ca++ transporting, plasma membrane 1b plasma membrane calcium ATPase 1 pmca1b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021470501.1	XP_021326176.1	plasma membrane calcium-transporting ATPase 3 isoform X2
XM_009297908.2	XP_009296183.1	plasma membrane calcium-transporting ATPase 3 isoform X1
XM_009297908.3	XP_009296183.1	plasma membrane calcium-transporting ATPase 3 isoform X1
XM_009297909.1	XP_009296184.1	plasma membrane calcium-transporting ATPase 3 isoform X1
NM_001135631.1	NP_001129103.1	plasma membrane calcium-transporting ATPase 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP2B4	XP_005640818.1
Arabidopsis thaliana (thale cress)	ACA10	NP_194719.1
Magnaporthe oryzae (rice blast fungus)	MGG_04890	XP_003712375.1
Macaca mulatta (Rhesus monkey)	ATP2B4	NP_001028098.1
Mus musculus (house mouse)	Atp2b4	NP_998781.1
Rattus norvegicus (Norway rat)	Atp2b4	NP_001005871.1
Schizosaccharomyces pombe (fission yeast)	SPAPB2B4.04c	NP_593890.1
Oryza sativa (rice)	Os04g0605500	NP_001053795.1
Arabidopsis thaliana (thale cress)	ACA8	NP_200521.3
Homo sapiens (human)	ATP2B4	NP_001675.3
Gallus gallus (chicken)	ATP2B4	XP_004948503.1
Arabidopsis thaliana (thale cress)	ACA9	NP_188755.2
Xenopus tropicalis (tropical clawed frog)	atp2b4	XP_004910734.1
Neurospora crassa	NCU05154	XP_956652.1
Pan troglodytes (chimpanzee)	ATP2B4	XP_001156333.2
Bos taurus (cattle)	ATP2B4	NP_001166065.1
Danio rerio (zebrafish)	atp2b1b	NP_001129103.1

Gene Ontology
Bibliography

Related articles in PubMed

Epigenetic regulation of hematopoiesis by DNA methylation.
Gore AV, Athans B, Iben JR, Johnson K, Russanova V, Castranova D, Pham VN, Butler MG, Williams-Simons L, Nichols JT, Bresciani E, Feldman B, Kimmel CB, Liu PP, Weinstein BM
eLife5e11813(2016 Jan)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The vertebrate ancestral repertoire of visual opsins, transducin alpha subunits and oxytocin/vasopressin receptors was established by duplication of their shared genomic region in the two rounds of early vertebrate genome duplications.
Lagman D, Ocampo Daza D, Widmark J, Abalo XM, Sundström G, Larhammar D
BMC evolutionary biology13238(2013 Nov)

Effects of dietary methylmercury on the zebrafish brain: histological, mitochondrial, and gene transcription analyses.
Cambier S, Gonzalez P, Mesmer-Dudons N, Brèthes D, Fujimura M, Bourdineaud JP
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine25(1)165-80(2012 Feb)

Expression and water calcium dependence of calcium transporter isoforms in zebrafish gill mitochondrion-rich cells.
Liao BK, Deng AN, Chen SC, Chou MY, Hwang PP
BMC genomics8354(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following atp2b1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atp2b1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa43456	XM_021470501.1 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 1b (atp2b1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa34209	NM_001135631.1 Latest version!	Danio rerio ATPase plasma membrane Ca2+ transporting 1b (atp2b1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa43455	XM_009297908.3 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 1b (atp2b1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa43455	XM_009297909.1 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 1b (atp2b1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa43455	XM_009297908.2	Danio rerio ATPase, Ca++ transporting, plasma membrane 1b (atp2b1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

atp2b1b ( XM_021470501.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326176.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 1b (atp2b1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa43456
Clone ID Related Accession (Same CDS sequence)	XM_021470501.1
Accession Version	XM_021470501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium-transporting ATPase 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCC TCAACAGTGA TGATGGAACA GAAGAAGAGC AGAAAAAAGC CAAGATACCA 
AAGAAAGAAA AATCGGTCCT TCAAGGAAAG CTTACCAAAC TAGCTGTGCA AATTGGAAAA 
GCGGGACTCT TCATGTCGGC CATCACCGTC CTCATTCTTG TGGTGCTCTT TTTGGTGGAC 
ACTTTCTGGA TCCAAGGGCT TCCATGGATC AAAGACTGCA CTCCTATCTA CATCCAGTTC 
TTTGTGAAGT TCTTTATTAT CGGCGTCACT GTGTTGGTTG TTGCTGTGCC TGAAGGTTTA 
CCTCTTGCCG TCACAATCTC ATTGGCATAT TCTGTGAAGA AAATGATGAA GGACAATAAC 
CTGGTGAGAC ATCTGGACGC TTGTGAGACG ATGGGCAATG CTACCGCCAT TTGCTCGGAT 
AAAACGGGCA CCCTAACTAT GAACAGGATG ACAGTGGTTC AGGTGTTTAT TGCAGGCAGA 
CACTTCAAGA AGGTTCCAGA GCCCGATCTG ATTCCTGGAA ACATCATGAA TCTGCTGGTC 
ACAGGCATAG GCGTTAACTG TGCATACACT TCAAAAATCA TGTCTGCTGA AAAAGAGGGT 
GGCCTGCCCC GTCAAGTTGG GAACAAAACT GAATGTGCCT TGCTAGGTTT TGTCACTGAT 
TTACGTAAGG ATTACCAAGC CATTCGCTGT GAATATCCTG AGGAAAAACT GTACAAAGTG 
TACACCTTTA ACTCAGTCAG GAAATCCATG AGTACAGTCC TGAAAAATTC TGATGGAAGT 
TACCGCATGT TTAGCAAGGG AGCTTCAGAA ATCCTCCTAA AGAAATGCTG CAAAATCCTG 
ACAAGCAATG GTGATGCCAA ACATTTCAGA CCCACTGATC GAGATGACAT GGTGACACAG 
GTGATAGAGC CTATGGCCTC TGAGGGATTG AGGACCATCT GCCTTGCCTA CAGGGACTTC 
CTTGTTTCGG ATGGGGAGCC TGACTGGAAT AATGAGGGTG ATATCCTAAC TGGACTGACC 
TGCATCTGTG TGGTGGGCAT TGAGGATCCT GTAAGACCAG AGGTCCCTGA TGCAATTAAA 
AAGTGTCAGC GAGCTGGGAT CACTGTGCGG ATGGTCACAG GGGATAATAT TAACACTGCT 
CGAGCCATAG CAACAAAGTG TGGCATTCTC CATATCGGGG ATGACTTCCT GTGCCTTGAA 
GGCAAAGAAT TCAACAGGCG GATACGGAAT GAACTAGGAG AGATTGAGCA AGAACGATTG 
GACAAGGTTT GGCCCAAACT GCGGGTACTT GCAAGATCCT CTCCTACTGA TAAGCATACG 
TTGGTTAAAG GTATAATCGA TAGCACCGTC TTAGAACAGA GACAAGTAGT AGCTGTCACT 
GGAGATGGAA CCAATGATGG TCCTGCGCTC AAAAAAGCTG ATGTTGGCTT TGCGATGGGG 
ATTGCAGGGA CAGATGTTGC TAAAGAAGCA TCAGACATCA TCCTCACCGA TGACAACTTC 
AGCAGCATCG TGAAAGCAGT AATGTGGGGG AGGAACGTTT ACGACAGCAT TTCGAAGTTC 
CTGCAATTTC AGTTGACAGT CAATGTGGTG GCTGTCATAG TGGCGTTTAC CGGAGCCTGT 
ATCACACAGG ACTCCCCTCT TAAAGCTGTA CAGATGCTCT GGGTGAATTT AATCATGGAC 
ACATTTGCAT CACTAGCTCT AGCAACAGAA CCTCCCACTG AATCTTTACT GTTGCGCAAA 
CCCTATGGTC GCAATAAGCC CCTTATTTCC CGCACCATGA TGAAGAACAT CCTTGGACAT 
GCTGTATATC AGCTGACCAT CATCTTCACA CTACTGTTTG CAGGAGAGCA GATCTTTGAT 
ATTGACAGTG GCAGGAACGC TCCTCTTCAC GCTCCTCCTT CAGAGCACTA CACCGTTGTC 
TTTAACACTT TCGTGCTGAT GCAGCTCTTC AATGAGATTA ACGCTCGAAA GATCCATGGA 
GAGAGAAATG TTTTCGAGGG TATCTTCAAT AACATAATCT TCTGCAGCAT TTTATTTGGG 
ACCTTTATTA TCCAGTTTGT AATTGTGCAG TTTGGAGGGA AGCCATTTAG CTGTGTTGGT 
CTCTCCGTTG AGCAGTGGCT GTGGTGCGTT TTCCTTGGTT TTGGATCTCT TCTATGGGGA 
CAGGTGATTT CCACCGTTCC CACAAGACGA CTGAAGTTCC TGATAACCAT GGGTCATGGC 
ACTAAGAAGG AGGAGATCCC CGAGGACGAG CTGGAGGAGA TGGAAGACCT GGATGAAATC 
GACCATGCAG AAATGGAGCT GAGAAGGGGG CAGGTTCTGT GGTGCAGGGG TCTCAACAGG 
ATCCAGACAC AGATCCGTGT GGTTAATGCT TTCCGGGATT CCATGTCCCC ATACGACGGC 
CTTGAGACGC CTGAGTCTCG CAGCTCTATT CACAATTTCA TGAGTCATCC GGAGTTTCGC 
ATCGAGGATT CAGAACCAGC GATTCCTCTA ATCGACGACA CAGATCCTGA GGAGGACGCA 
CCCACCAAAC GCAACGCAGC TCCTTCTCCA CTGCCTCTAG TTTTATCTTC CCCCAATCAG 
AACAATAACG CCATCGATGG CAGGGGCTTC CATCACCACA TAGATCTAGC AAAAACTATC 
CTTCCTCCCA CTCCTGGAAG CCCATTACAC AGCCTGGAGA CATCGCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326176.1
CDS	102..2792
Translation	MEPLNSDDGTEEEQKKAKIPKKEKSVLQGKLTKLAVQIGKAGLFMSAITVLILVVLFLVDTFWIQGLPWIKDCTPIYIQFFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQVFIAGRHFKKVPEPDLIPGNIMNLLVTGIGVNCAYTSKIMSAEKEGGLPRQVGNKTECALLGFVTDLRKDYQAIRCEYPEEKLYKVYTFNSVRKSMSTVLKNSDGSYRMFSKGASEILLKKCCKILTSNGDAKHFRPTDRDDMVTQVIEPMASEGLRTICLAYRDFLVSDGEPDWNNEGDILTGLTCICVVGIEDPVRPEVPDAIKKCQRAGITVRMVTGDNINTARAIATKCGILHIGDDFLCLEGKEFNRRIRNELGEIEQERLDKVWPKLRVLARSSPTDKHTLVKGIIDSTVLEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAVYQLTIIFTLLFAGEQIFDIDSGRNAPLHAPPSEHYTVVFNTFVLMQLFNEINARKIHGERNVFEGIFNNIIFCSILFGTFIIQFVIVQFGGKPFSCVGLSVEQWLWCVFLGFGSLLWGQVISTVPTRRLKFLITMGHGTKKEEIPEDELEEMEDLDEIDHAEMELRRGQVLWCRGLNRIQTQIRVVNAFRDSMSPYDGLETPESRSSIHNFMSHPEFRIEDSEPAIPLIDDTDPEEDAPTKRNAAPSPLPLVLSSPNQNNNAIDGRGFHHHIDLAKTILPPTPGSPLHSLETSL

Target ORF information:

RefSeq Version	XM_021470501.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 1b (atp2b1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470501.1

ORF Insert Sequence:

ATGGAGCCCC TCAACAGTGA TGATGGAACA GAAGAAGAGC AGAAAAAAGC CAAGATACCA 
AAGAAAGAAA AATCGGTCCT TCAAGGAAAG CTTACCAAAC TAGCTGTGCA AATTGGAAAA 
GCGGGACTCT TCATGTCGGC CATCACCGTC CTCATTCTTG TGGTGCTCTT TTTGGTGGAC 
ACTTTCTGGA TCCAAGGGCT TCCATGGATC AAAGACTGCA CTCCTATCTA CATCCAGTTC 
TTTGTGAAGT TCTTTATTAT CGGCGTCACT GTGTTGGTTG TTGCTGTGCC TGAAGGTTTA 
CCTCTTGCCG TCACAATCTC ATTGGCATAT TCTGTGAAGA AAATGATGAA GGACAATAAC 
CTGGTGAGAC ATCTGGACGC TTGTGAGACG ATGGGCAATG CTACCGCCAT TTGCTCGGAT 
AAAACGGGCA CCCTAACTAT GAACAGGATG ACAGTGGTTC AGGTGTTTAT TGCAGGCAGA 
CACTTCAAGA AGGTTCCAGA GCCCGATCTG ATTCCTGGAA ACATCATGAA TCTGCTGGTC 
ACAGGCATAG GCGTTAACTG TGCATACACT TCAAAAATCA TGTCTGCTGA AAAAGAGGGT 
GGCCTGCCCC GTCAAGTTGG GAACAAAACT GAATGTGCCT TGCTAGGTTT TGTCACTGAT 
TTACGTAAGG ATTACCAAGC CATTCGCTGT GAATATCCTG AGGAAAAACT GTACAAAGTG 
TACACCTTTA ACTCAGTCAG GAAATCCATG AGTACAGTCC TGAAAAATTC TGATGGAAGT 
TACCGCATGT TTAGCAAGGG AGCTTCAGAA ATCCTCCTAA AGAAATGCTG CAAAATCCTG 
ACAAGCAATG GTGATGCCAA ACATTTCAGA CCCACTGATC GAGATGACAT GGTGACACAG 
GTGATAGAGC CTATGGCCTC TGAGGGATTG AGGACCATCT GCCTTGCCTA CAGGGACTTC 
CTTGTTTCGG ATGGGGAGCC TGACTGGAAT AATGAGGGTG ATATCCTAAC TGGACTGACC 
TGCATCTGTG TGGTGGGCAT TGAGGATCCT GTAAGACCAG AGGTCCCTGA TGCAATTAAA 
AAGTGTCAGC GAGCTGGGAT CACTGTGCGG ATGGTCACAG GGGATAATAT TAACACTGCT 
CGAGCCATAG CAACAAAGTG TGGCATTCTC CATATCGGGG ATGACTTCCT GTGCCTTGAA 
GGCAAAGAAT TCAACAGGCG GATACGGAAT GAACTAGGAG AGATTGAGCA AGAACGATTG 
GACAAGGTTT GGCCCAAACT GCGGGTACTT GCAAGATCCT CTCCTACTGA TAAGCATACG 
TTGGTTAAAG GTATAATCGA TAGCACCGTC TTAGAACAGA GACAAGTAGT AGCTGTCACT 
GGAGATGGAA CCAATGATGG TCCTGCGCTC AAAAAAGCTG ATGTTGGCTT TGCGATGGGG 
ATTGCAGGGA CAGATGTTGC TAAAGAAGCA TCAGACATCA TCCTCACCGA TGACAACTTC 
AGCAGCATCG TGAAAGCAGT AATGTGGGGG AGGAACGTTT ACGACAGCAT TTCGAAGTTC 
CTGCAATTTC AGTTGACAGT CAATGTGGTG GCTGTCATAG TGGCGTTTAC CGGAGCCTGT 
ATCACACAGG ACTCCCCTCT TAAAGCTGTA CAGATGCTCT GGGTGAATTT AATCATGGAC 
ACATTTGCAT CACTAGCTCT AGCAACAGAA CCTCCCACTG AATCTTTACT GTTGCGCAAA 
CCCTATGGTC GCAATAAGCC CCTTATTTCC CGCACCATGA TGAAGAACAT CCTTGGACAT 
GCTGTATATC AGCTGACCAT CATCTTCACA CTACTGTTTG CAGGAGAGCA GATCTTTGAT 
ATTGACAGTG GCAGGAACGC TCCTCTTCAC GCTCCTCCTT CAGAGCACTA CACCGTTGTC 
TTTAACACTT TCGTGCTGAT GCAGCTCTTC AATGAGATTA ACGCTCGAAA GATCCATGGA 
GAGAGAAATG TTTTCGAGGG TATCTTCAAT AACATAATCT TCTGCAGCAT TTTATTTGGG 
ACCTTTATTA TCCAGTTTGT AATTGTGCAG TTTGGAGGGA AGCCATTTAG CTGTGTTGGT 
CTCTCCGTTG AGCAGTGGCT GTGGTGCGTT TTCCTTGGTT TTGGATCTCT TCTATGGGGA 
CAGGTGATTT CCACCGTTCC CACAAGACGA CTGAAGTTCC TGATAACCAT GGGTCATGGC 
ACTAAGAAGG AGGAGATCCC CGAGGACGAG CTGGAGGAGA TGGAAGACCT GGATGAAATC 
GACCATGCAG AAATGGAGCT GAGAAGGGGG CAGGTTCTGT GGTGCAGGGG TCTCAACAGG 
ATCCAGACAC AGATCCGTGT GGTTAATGCT TTCCGGGATT CCATGTCCCC ATACGACGGC 
CTTGAGACGC CTGAGTCTCG CAGCTCTATT CACAATTTCA TGAGTCATCC GGAGTTTCGC 
ATCGAGGATT CAGAACCAGC GATTCCTCTA ATCGACGACA CAGATCCTGA GGAGGACGCA 
CCCACCAAAC GCAACGCAGC TCCTTCTCCA CTGCCTCTAG TTTTATCTTC CCCCAATCAG 
AACAATAACG CCATCGATGG CAGGGGCTTC CATCACCACA TAGATCTAGC AAAAACTATC 
CTTCCTCCCA CTCCTGGAAG CCCATTACAC AGCCTGGAGA CATCGCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b1b ( NM_001135631.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001129103.1 Danio rerio ATPase plasma membrane Ca2+ transporting 1b (atp2b1b), mRNA.

Price & Availability↑

CloneID	ODa34209
Clone ID Related Accession (Same CDS sequence)	NM_001135631.1
Accession Version	NM_001135631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium-transporting ATPase 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU559281.1. On Oct 4, 2008 this sequence version replaced XM_001922991.1. ##Evidence-Data-START## Transcript exon combination :: EU559281.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCCAAAA ACTCATTCCG TGGGTCGAAG TACAAGCCAG AGGCCAATCA TGATGGACAT 
TTTGGCTGCA CACTCAAAGA GCTTCGTTCT CTTATGGAAC TCAGAGGAAC GGATGGATTA 
CAAAGAATTC AGGAGTGCTA TGGAGATGTT CAAGGACTCT GCTCCAAGTT AAAATCATCA 
CCGATAGAAG GCTTAAGTGG TCATCCCGAT GACATTGCAC GAAGAAAAGA AGAGTTCGGA 
AAAAATTTTA TACCTCCTAA AAAGCCAAAA ACATTCCTTC AATTAGTGTG GGAAGCACTG 
CAGGATGTGA CTCTAATTAT TTTGGAAGTT GCAGCCATAA TATCCTTAGG CCTTTCCTTT 
TATAAACCTC CCGATGCCGA GAGAGAACAC TGTGGTCGGG CGGCTGGAGG TGTCGAGGAT 
GAGGGGGAGG CAGAAGCGGG CTGGATTGAA GGTGCTGCCA TCCTCCTGTC TGTCGTCTGC 
GTGGTTCTTG TCACAGCTTT TAACGACTGG AGTAAAGAGA AACAGTTTCG TGGACTCCAA 
AGCCGGATTG AACAAGAGCA GAGGTTTACA GTACTCAGAG GCGGTCAAGT CATTCAGATT 
CCAGTGTCCG AGATTGTGGT CGGAGACATC GCACAAGTTA AATATGGTGA CCTCCTTCCT 
GCTGATGGTG TACTAATCCA AGGCAACGAT CTAAAAATTG ATGAGAGCTC CCTCACTGGA 
GAGTCGGACC ACGTCAAAAA AACTCTGGAC AAAGACCCCA TTCTTCTTTC AGGCACACAT 
GTAATGGAGG GCTCCGGAAA AATGCTGGTC ACTGCAGTGG GGGTTAATTC TCAAACGGGA 
ATTATTTTCA TGCTGCTCGG AGGTGGAGAT GACGATGACG ACGATGATGA GGAGAAAAAG 
GAAGAGAGGG AGAGGAAAAA GAGGGAGAAG GAAGAGAAGA GGAGAGAAAA GGAGGAGAAA 
AAGAAAGAGA AAGAGAGGAA GAAGAAGGAG CAGAAAGACA AAAAGAAAAA GAAAGATGGA 
GGTACAGTAG AAATGGAGCC CCTCAACAGT GATGATGGAA CAGAAGAAGA GCAGAAAAAA 
GCCAAGATAC CAAAGAAAGA AAAATCGGTC CTTCAAGGAA AGCTTACCAA ACTAGCTGTG 
CAAATTGGAA AAGCGGGACT CTTCATGTCG GCCATCACCG TCCTCATTCT TGTGGTGCTC 
TTTTTGGTGG ACACTTTCTG GATCCAAGGG CTTCCATGGA TCAAAGACTG CACTCCTATC 
TACATCCAGT TCTTTGTGAA GTTCTTTATT ATCGGCGTCA CTGTGTTGGT TGTTGCTGTG 
CCTGAAGGTT TACCTCTTGC CGTCACAATC TCATTGGCAT ATTCTGTGAA GAAAATGATG 
AAGGACAATA ACCTGGTGAG ACATCTGGAC GCTTGTGAGA CGATGGGCAA TGCTACCGCC 
ATTTGCTCGG ATAAAACGGG CACCCTAACT ATGAACAGGA TGACAGTGGT TCAGGTGTTT 
ATTGCAGGCA GACACTTCAA GAAGGTTCCA GAGCCCGATC TGATTCCTGG AAACATCATG 
AATCTGCTGG TCACAGGCAT AGGCGTTAAC TGTGCATACA CTTCAAAAAT CATGTCTGCT 
GAAAAAGAGG GTGGCCTGCC CCGTCAAGTT GGGAACAAAA CTGAATGTGC CTTGCTAGGT 
TTTGTCACTG ATTTACGTAA GGATTACCAA GCCATTCGCT GTGAATATCC TGAGGAAAAA 
CTGTACAAAG TGTACACCTT TAACTCAGTC AGGAAATCCA TGAGTACAGT CCTGAAAAAT 
TCTGATGGAA GTTACCGCAT GTTTAGCAAG GGAGCTTCAG AAATCCTCCT AAAGAAATGC 
TGCAAAATCC TGACAAGCAA TGGTGATGCC AAACATTTCA GACCCACTGA TCGAGATGAC 
ATGGTGACAC AGGTGATAGA GCCTATGGCC TCTGAGGGAT TGAGGACCAT CTGCCTTGCC 
TACAGGGACT TCCTTGTTTC GGATGGGGAG CCTGACTGGA ATAATGAGGG TGATATCCTA 
ACTGGACTGA CCTGCATCTG TGTGGTGGGC ATTGAGGATC CTGTAAGACC AGAGGTCCCT 
GATGCAATTA AAAAGTGTCA GCGAGCTGGG ATCACTGTGC GGATGGTCAC AGGGGATAAT 
ATTAACACTG CTCGAGCCAT AGCAACAAAG TGTGGCATTC TCCATATCGG GGATGACTTC 
CTGTGCCTTG AAGGCAAAGA ATTCAACAGG CGGATACGGA ATGAACTAGG AGAGATTGAG 
CAAGAACGAT TGGACAAGGT TTGGCCCAAA CTGCGGGTAC TTGCAAGATC CTCTCCTACT 
GATAAGCATA CGTTGGTTAA AGGTATAATC GATAGCACCG TCTTAGAACA GAGACAAGTA 
GTAGCTGTCA CTGGAGATGG AACCAATGAT GGTCCTGCGC TCAAAAAAGC TGATGTTGGC 
TTTGCGATGG GGATTGCAGG GACAGATGTT GCTAAAGAAG CATCAGACAT CATCCTCACC 
GATGACAACT TCAGCAGCAT CGTGAAAGCA GTAATGTGGG GGAGGAACGT TTACGACAGC 
ATTTCGAAGT TCCTGCAATT TCAGTTGACA GTCAATGTGG TGGCTGTCAT AGTGGCGTTT 
ACCGGAGCCT GTATCACACA GGACTCCCCT CTTAAAGCTG TACAGATGCT CTGGGTGAAT 
TTAATCATGG ACACATTTGC ATCACTAGCT CTAGCAACAG AACCTCCCAC TGAATCTTTA 
CTGTTGCGCA AACCCTATGG TCGCAATAAG CCCCTTATTT CCCGCACCAT GATGAAGAAC 
ATCCTTGGAC ATGCTGTATA TCAGCTGACC ATCATCTTCA CACTACTGTT TGCAGGAGAG 
CAGATCTTTG ATATTGACAG TGGCAGGAAC GCTCCTCTTC ACGCTCCTCC TTCAGAGCAC 
TACACCGTTG TCTTTAACAC TTTCGTGCTG ATGCAGCTCT TCAATGAGAT TAACGCTCGA 
AAGATCCATG GAGAGAGAAA TGTTTTCGAG GGTATCTTCA ATAACATAAT CTTCTGCAGC 
ATTTTATTTG GGACCTTTAT TATCCAGTTT GTAATTGTGC AGTTTGGAGG GAAGCCATTT 
AGCTGTGTTG GTCTCTCCGT TGAGCAGTGG CTGTGGTGCG TTTTCCTTGG TTTTGGATCT 
CTTCTATGGG GACAGGTGAT TTCCACCGTT CCCACAAGAC GACTGAAGTT CCTGATAACC 
ATGGGTCATG GCACTAAGAA GGAGGAGATC CCCGAGGACG AGCTGGAGGA GATGGAAGAC 
CTGGATGAAA TCGACCATGC AGAAATGGAG CTGAGAAGGG GGCAGGTTCT GTGGTGCAGG 
GGTCTCAACA GGATCCAGAC ACAGATCCGT GTGGTTAATG CTTTCCGGGA TTCCATGTCC 
CCATACGACG GCCTTGAGAC GCCTGAGTCT CGCAGCTCTA TTCACAATTT CATGAGTCAT 
CCGGAGTTTC GCATCGAGGA TTCAGAACCA GCGATTCCTC TAATCGACGA CACAGATCCT 
GAGGAGGACG CACCCACCAA ACGCAACGCA GCTCCTTCTC CACTGCCTCT AGTTTTATCT 
TCCCCCAATC AGAACAATAA CGCCATCGAT GGCAGGGGCT TCCATCACCA CATAGATCTA 
GCAAAAACTA TCCTTCCTCC CACTCCTGGA AGCCCATTAC ACAGCCTGGA GACATCGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129103.1
CDS	256..3978
Translation	MAKNSFRGSKYKPEANHDGHFGCTLKELRSLMELRGTDGLQRIQECYGDVQGLCSKLKSSPIEGLSGHPDDIARRKEEFGKNFIPPKKPKTFLQLVWEALQDVTLIILEVAAIISLGLSFYKPPDAEREHCGRAAGGVEDEGEAEAGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQRFTVLRGGQVIQIPVSEIVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVKKTLDKDPILLSGTHVMEGSGKMLVTAVGVNSQTGIIFMLLGGGDDDDDDDEEKKEERERKKREKEEKRREKEEKKKEKERKKKEQKDKKKKKDGGTVEMEPLNSDDGTEEEQKKAKIPKKEKSVLQGKLTKLAVQIGKAGLFMSAITVLILVVLFLVDTFWIQGLPWIKDCTPIYIQFFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQVFIAGRHFKKVPEPDLIPGNIMNLLVTGIGVNCAYTSKIMSAEKEGGLPRQVGNKTECALLGFVTDLRKDYQAIRCEYPEEKLYKVYTFNSVRKSMSTVLKNSDGSYRMFSKGASEILLKKCCKILTSNGDAKHFRPTDRDDMVTQVIEPMASEGLRTICLAYRDFLVSDGEPDWNNEGDILTGLTCICVVGIEDPVRPEVPDAIKKCQRAGITVRMVTGDNINTARAIATKCGILHIGDDFLCLEGKEFNRRIRNELGEIEQERLDKVWPKLRVLARSSPTDKHTLVKGIIDSTVLEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAVYQLTIIFTLLFAGEQIFDIDSGRNAPLHAPPSEHYTVVFNTFVLMQLFNEINARKIHGERNVFEGIFNNIIFCSILFGTFIIQFVIVQFGGKPFSCVGLSVEQWLWCVFLGFGSLLWGQVISTVPTRRLKFLITMGHGTKKEEIPEDELEEMEDLDEIDHAEMELRRGQVLWCRGLNRIQTQIRVVNAFRDSMSPYDGLETPESRSSIHNFMSHPEFRIEDSEPAIPLIDDTDPEEDAPTKRNAAPSPLPLVLSSPNQNNNAIDGRGFHHHIDLAKTILPPTPGSPLHSLETSL

Target ORF information:

RefSeq Version	NM_001135631.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase plasma membrane Ca2+ transporting 1b (atp2b1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135631.1

ORF Insert Sequence:

ATGGCCAAAA ACTCATTCCG TGGGTCGAAG TACAAGCCAG AGGCCAATCA TGATGGACAT 
TTTGGCTGCA CACTCAAAGA GCTTCGTTCT CTTATGGAAC TCAGAGGAAC GGATGGATTA 
CAAAGAATTC AGGAGTGCTA TGGAGATGTT CAAGGACTCT GCTCCAAGTT AAAATCATCA 
CCGATAGAAG GCTTAAGTGG TCATCCCGAT GACATTGCAC GAAGAAAAGA AGAGTTCGGA 
AAAAATTTTA TACCTCCTAA AAAGCCAAAA ACATTCCTTC AATTAGTGTG GGAAGCACTG 
CAGGATGTGA CTCTAATTAT TTTGGAAGTT GCAGCCATAA TATCCTTAGG CCTTTCCTTT 
TATAAACCTC CCGATGCCGA GAGAGAACAC TGTGGTCGGG CGGCTGGAGG TGTCGAGGAT 
GAGGGGGAGG CAGAAGCGGG CTGGATTGAA GGTGCTGCCA TCCTCCTGTC TGTCGTCTGC 
GTGGTTCTTG TCACAGCTTT TAACGACTGG AGTAAAGAGA AACAGTTTCG TGGACTCCAA 
AGCCGGATTG AACAAGAGCA GAGGTTTACA GTACTCAGAG GCGGTCAAGT CATTCAGATT 
CCAGTGTCCG AGATTGTGGT CGGAGACATC GCACAAGTTA AATATGGTGA CCTCCTTCCT 
GCTGATGGTG TACTAATCCA AGGCAACGAT CTAAAAATTG ATGAGAGCTC CCTCACTGGA 
GAGTCGGACC ACGTCAAAAA AACTCTGGAC AAAGACCCCA TTCTTCTTTC AGGCACACAT 
GTAATGGAGG GCTCCGGAAA AATGCTGGTC ACTGCAGTGG GGGTTAATTC TCAAACGGGA 
ATTATTTTCA TGCTGCTCGG AGGTGGAGAT GACGATGACG ACGATGATGA GGAGAAAAAG 
GAAGAGAGGG AGAGGAAAAA GAGGGAGAAG GAAGAGAAGA GGAGAGAAAA GGAGGAGAAA 
AAGAAAGAGA AAGAGAGGAA GAAGAAGGAG CAGAAAGACA AAAAGAAAAA GAAAGATGGA 
GGTACAGTAG AAATGGAGCC CCTCAACAGT GATGATGGAA CAGAAGAAGA GCAGAAAAAA 
GCCAAGATAC CAAAGAAAGA AAAATCGGTC CTTCAAGGAA AGCTTACCAA ACTAGCTGTG 
CAAATTGGAA AAGCGGGACT CTTCATGTCG GCCATCACCG TCCTCATTCT TGTGGTGCTC 
TTTTTGGTGG ACACTTTCTG GATCCAAGGG CTTCCATGGA TCAAAGACTG CACTCCTATC 
TACATCCAGT TCTTTGTGAA GTTCTTTATT ATCGGCGTCA CTGTGTTGGT TGTTGCTGTG 
CCTGAAGGTT TACCTCTTGC CGTCACAATC TCATTGGCAT ATTCTGTGAA GAAAATGATG 
AAGGACAATA ACCTGGTGAG ACATCTGGAC GCTTGTGAGA CGATGGGCAA TGCTACCGCC 
ATTTGCTCGG ATAAAACGGG CACCCTAACT ATGAACAGGA TGACAGTGGT TCAGGTGTTT 
ATTGCAGGCA GACACTTCAA GAAGGTTCCA GAGCCCGATC TGATTCCTGG AAACATCATG 
AATCTGCTGG TCACAGGCAT AGGCGTTAAC TGTGCATACA CTTCAAAAAT CATGTCTGCT 
GAAAAAGAGG GTGGCCTGCC CCGTCAAGTT GGGAACAAAA CTGAATGTGC CTTGCTAGGT 
TTTGTCACTG ATTTACGTAA GGATTACCAA GCCATTCGCT GTGAATATCC TGAGGAAAAA 
CTGTACAAAG TGTACACCTT TAACTCAGTC AGGAAATCCA TGAGTACAGT CCTGAAAAAT 
TCTGATGGAA GTTACCGCAT GTTTAGCAAG GGAGCTTCAG AAATCCTCCT AAAGAAATGC 
TGCAAAATCC TGACAAGCAA TGGTGATGCC AAACATTTCA GACCCACTGA TCGAGATGAC 
ATGGTGACAC AGGTGATAGA GCCTATGGCC TCTGAGGGAT TGAGGACCAT CTGCCTTGCC 
TACAGGGACT TCCTTGTTTC GGATGGGGAG CCTGACTGGA ATAATGAGGG TGATATCCTA 
ACTGGACTGA CCTGCATCTG TGTGGTGGGC ATTGAGGATC CTGTAAGACC AGAGGTCCCT 
GATGCAATTA AAAAGTGTCA GCGAGCTGGG ATCACTGTGC GGATGGTCAC AGGGGATAAT 
ATTAACACTG CTCGAGCCAT AGCAACAAAG TGTGGCATTC TCCATATCGG GGATGACTTC 
CTGTGCCTTG AAGGCAAAGA ATTCAACAGG CGGATACGGA ATGAACTAGG AGAGATTGAG 
CAAGAACGAT TGGACAAGGT TTGGCCCAAA CTGCGGGTAC TTGCAAGATC CTCTCCTACT 
GATAAGCATA CGTTGGTTAA AGGTATAATC GATAGCACCG TCTTAGAACA GAGACAAGTA 
GTAGCTGTCA CTGGAGATGG AACCAATGAT GGTCCTGCGC TCAAAAAAGC TGATGTTGGC 
TTTGCGATGG GGATTGCAGG GACAGATGTT GCTAAAGAAG CATCAGACAT CATCCTCACC 
GATGACAACT TCAGCAGCAT CGTGAAAGCA GTAATGTGGG GGAGGAACGT TTACGACAGC 
ATTTCGAAGT TCCTGCAATT TCAGTTGACA GTCAATGTGG TGGCTGTCAT AGTGGCGTTT 
ACCGGAGCCT GTATCACACA GGACTCCCCT CTTAAAGCTG TACAGATGCT CTGGGTGAAT 
TTAATCATGG ACACATTTGC ATCACTAGCT CTAGCAACAG AACCTCCCAC TGAATCTTTA 
CTGTTGCGCA AACCCTATGG TCGCAATAAG CCCCTTATTT CCCGCACCAT GATGAAGAAC 
ATCCTTGGAC ATGCTGTATA TCAGCTGACC ATCATCTTCA CACTACTGTT TGCAGGAGAG 
CAGATCTTTG ATATTGACAG TGGCAGGAAC GCTCCTCTTC ACGCTCCTCC TTCAGAGCAC 
TACACCGTTG TCTTTAACAC TTTCGTGCTG ATGCAGCTCT TCAATGAGAT TAACGCTCGA 
AAGATCCATG GAGAGAGAAA TGTTTTCGAG GGTATCTTCA ATAACATAAT CTTCTGCAGC 
ATTTTATTTG GGACCTTTAT TATCCAGTTT GTAATTGTGC AGTTTGGAGG GAAGCCATTT 
AGCTGTGTTG GTCTCTCCGT TGAGCAGTGG CTGTGGTGCG TTTTCCTTGG TTTTGGATCT 
CTTCTATGGG GACAGGTGAT TTCCACCGTT CCCACAAGAC GACTGAAGTT CCTGATAACC 
ATGGGTCATG GCACTAAGAA GGAGGAGATC CCCGAGGACG AGCTGGAGGA GATGGAAGAC 
CTGGATGAAA TCGACCATGC AGAAATGGAG CTGAGAAGGG GGCAGGTTCT GTGGTGCAGG 
GGTCTCAACA GGATCCAGAC ACAGATCCGT GTGGTTAATG CTTTCCGGGA TTCCATGTCC 
CCATACGACG GCCTTGAGAC GCCTGAGTCT CGCAGCTCTA TTCACAATTT CATGAGTCAT 
CCGGAGTTTC GCATCGAGGA TTCAGAACCA GCGATTCCTC TAATCGACGA CACAGATCCT 
GAGGAGGACG CACCCACCAA ACGCAACGCA GCTCCTTCTC CACTGCCTCT AGTTTTATCT 
TCCCCCAATC AGAACAATAA CGCCATCGAT GGCAGGGGCT TCCATCACCA CATAGATCTA 
GCAAAAACTA TCCTTCCTCC CACTCCTGGA AGCCCATTAC ACAGCCTGGA GACATCGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b1b ( XM_009297908.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296183.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 1b (atp2b1b), transcript variant X1, mRNA.

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CloneID	ODa43455
Clone ID Related Accession (Same CDS sequence)	XM_009297908.2 , XM_009297908.3 , XM_009297909.1
Accession Version	XM_009297908.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	plasma membrane calcium-transporting ATPase 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAA ACTCATTCCG TGGGTCGAAG TACAAGCCAG AGGCCAATCA TGATGGACAT 
TTTGGCTGCA CACTCAAAGA GCTTCGTTCT CTTATGGAAC TCAGAGGAAC GGATGGATTA 
CAAAGAATTC AGGAGTGCTA TGGAGATGTT CAAGGACTCT GCTCCAAGTT AAAATCATCA 
CCGATAGAAG GCTTAAGTGG TCATCCCGAT GACATTGCAC GAAGAAAAGA AGAGTTCGGA 
AAAAATTTTA TACCTCCTAA AAAGCCAAAA ACATTCCTTC AATTAGTGTG GGAAGCACTG 
CAGGATGTGA CTCTAATTAT TTTGGAAGTT GCAGCCATAA TATCCTTAGG CCTTTCCTTT 
TATAAACCTC CCGATGCCGA GAGAGAACAC TGTGGTCGGG CGGCTGGAGG TGTCGAGGAT 
GAGGGGGAGG CAGAAGCGGG CTGGATTGAA GGTGCTGCCA TCCTCCTGTC TGTCGTCTGC 
GTGGTTCTTG TCACAGCTTT TAACGACTGG AGTAAAGAGA AACAGTTTCG TGGACTCCAA 
AGCCGGATTG AACAAGAGCA GAGGTTTACA GTACTCAGAG GCGGTCAAGT CATTCAGATA 
CCAGTGTCCG AGATTGTGGT CGGAGACATC GCACAAGTTA AATATGGTGA CCTCCTTCCT 
GCTGATGGTG TACTAATCCA AGGCAACGAT CTAAAAATTG ATGAGAGCTC CCTCACTGGA 
GAGTCGGATC ACGTCAAAAA AACTCTGGAC AAAGACCCCA TTCTTCTTTC AGGCACACAT 
GTAATGGAGG GCTCCGGAAA AATGCTGGTC ACTGCAGTGG GGGTTAATTC TCAAACGGGA 
ATTATTTTCA TGCTGCTCGG AGGTGGAGAT GACGATGACG ACGATGATGA GGAGAAAAAG 
GAAGAGAAGG AGAGGAAAAA GAGGGAGAAG GAAGAGAAGA GGAGAGAAAA GGAGGAGAAA 
AAGAAAGAGA AAGAGAGGAA GAAGAAGGAG CAGAAAGACA AAAAGAAAAA GAAAGATGGA 
GGTACAGTAG AAATGGAGCC CCTCAACAGT GATGATGGAA CAGAAGAAGA GCAGAAAAAA 
GCCAAGATAC CAAAGAAAGA AAAATCGGTC CTTCAAGGAA AGCTTACCAA ACTAGCTGTG 
CAAATTGGAA AAGCGGGACT CTTCATGTCG GCCATCACCG TCCTCATTCT TGTGGTGCTC 
TTTTTGGTGG ACACTTTCTG GATCCAAGGG CTTCCATGGA TCAAAGACTG CACTCCTATC 
TACATCCAGT TCTTTGTGAA GTTCTTTATT ATCGGCGTCA CTGTGTTGGT TGTTGCTGTG 
CCTGAAGGTT TACCTCTTGC CGTCACAATC TCATTGGCAT ATTCTGTGAA GAAAATGATG 
AAGGACAATA ACCTGGTGAG ACATCTGGAC GCTTGTGAGA CGATGGGCAA TGCTACCGCC 
ATTTGCTCGG ATAAAACGGG CACCCTAACT ATGAACAGGA TGACAGTGGT TCAGGTGTTT 
ATTGCAGGCA GACACTTCAA GAAGGTTCCA GAGCCCGATC TGATTCCTGG AAACATCATG 
AATCTGCTGG TCACAGGCAT AGGCGTTAAC TGTGCATACA CTTCAAAAAT CATGTCTGCT 
GAAAAAGAGG GTGGCCTGCC CCGTCAAGTT GGGAACAAAA CTGAATGTGC CTTGCTAGGT 
TTTGTCACTG ATTTACGTAA GGATTACCAA GCCATTCGCT GTGAATATCC TGAGGAAAAA 
CTGTACAAAG TGTACACCTT TAACTCAGTC AGGAAATCCA TGAGTACAGT CCTGAAAAAT 
TCTGATGGAA GTTACCGCAT GTTTAGCAAG GGAGCTTCAG AAATCCTCCT AAAGAAATGC 
TGCAAAATCC TGACAAGCAA TGGTGATGCC AAACATTTCA GACCCACTGA TCGAGATGAC 
ATGGTGACAC AGGTGATAGA GCCTATGGCC TCTGAGGGAT TGAGGACCAT CTGCCTTGCC 
TACAGGGACT TCCTTGTTTC GGATGGGGAG CCTGACTGGA ATAATGAGGG TGATATCCTA 
ACTGGACTGA CCTGCATCTG TGTGGTGGGC ATTGAGGATC CTGTAAGACC AGAGGTCCCT 
GATGCAATTA AAAAGTGTCA GCGAGCTGGG ATCACTGTGC GGATGGTCAC AGGGGATAAT 
ATTAACACTG CTCGAGCCAT AGCAACAAAG TGTGGCATTC TCCATATCGG GGATGACTTC 
CTGTGCCTTG AAGGCAAAGA ATTCAACAGG CGGATACGGA ATGAACTAGG AGAGATTGAG 
CAAGAACGAT TGGACAAGGT TTGGCCCAAA CTGCGGGTAC TTGCAAGATC CTCTCCTACT 
GATAAGCATA CGTTGGTTAA AGGTATAATC GATAGCACCG TCTTAGAACA GAGACAAGTA 
GTAGCTGTCA CTGGAGATGG AACCAATGAT GGTCCTGCGC TCAAAAAAGC TGATGTTGGC 
TTTGCGATGG GGATTGCAGG GACAGATGTT GCTAAAGAAG CATCAGACAT CATCCTCACC 
GATGACAACT TCAGCAGCAT CGTGAAAGCA GTAATGTGGG GGAGGAACGT TTACGACAGC 
ATTTCGAAGT TCCTGCAATT TCAGTTGACA GTCAATGTGG TGGCTGTCAT AGTGGCGTTT 
ACCGGAGCCT GTATCACACA GGACTCCCCT CTTAAAGCTG TACAGATGCT CTGGGTGAAT 
TTAATCATGG ACACATTTGC ATCACTAGCT CTAGCAACAG AACCTCCCAC TGAATCTTTA 
CTGTTGCGCA AACCCTATGG TCGCAATAAG CCCCTTATTT CCCGCACCAT GATGAAGAAC 
ATCCTTGGAC ATGCTGTATA TCAGCTGACC ATCATCTTCA CACTACTGTT TGCAGGAGAG 
CAGATCTTTG ATATTGACAG TGGCAGGAAC GCTCCTCTTC ACGCTCCTCC TTCAGAGCAC 
TACACCGTTG TCTTTAACAC TTTCGTGCTG ATGCAGCTCT TCAATGAGAT TAACGCTCGA 
AAGATCCATG GAGAGAGAAA TGTTTTCGAG GGTATCTTCA ATAACATAAT CTTCTGCAGC 
ATTTTATTTG GGACCTTTAT TATCCAGTTT GTAATTGTGC AGTTTGGAGG GAAGCCATTT 
AGCTGTGTTG GTCTCTCCGT TGAGCAGTGG CTGTGGTGCG TTTTCCTTGG TTTTGGATCT 
CTTCTATGGG GACAGGTGAT TTCCACCGTT CCCACAAGAC GACTGAAGTT CCTGATAACC 
ATGGGTCATG GCACTAAGAA GGAGGAGATC CCCGAGGACG AGCTGGAGGA GATGGAAGAC 
CTGGATGAAA TCGACCATGC AGAAATGGAG CTGAGAAGGG GGCAGGTTCT GTGGTGCAGG 
GGTCTCAACA GGATCCAGAC ACAGATCCGT GTGGTTAATG CTTTCCGGGA TTCCATGTCC 
CCATACGACG GCCTTGAGAC GCCTGAGTCT CGCAGCTCTA TTCACAATTT CATGAGTCAT 
CCGGAGTTTC GCATCGAGGA TTCAGAACCA GCGATTCCTC TAATCGACGA CACAGATCCT 
GAGGAGGACG CACCCACCAA ACGCAACGCA GCTCCTTCTC CACTGCCTCT AGTTTTATCT 
TCCCCCAATC AGAACAATAA CGCCATCGAT GGCAGGGGCT TCCATCACCA CATAGATCTA 
GCAAAAACTA TCCTTCCTCC CACTCCTGGA AGCCCATTAC ACAGCCTGGA GACATCGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296183.1
CDS	445..4167
Translation	MAKNSFRGSKYKPEANHDGHFGCTLKELRSLMELRGTDGLQRIQECYGDVQGLCSKLKSSPIEGLSGHPDDIARRKEEFGKNFIPPKKPKTFLQLVWEALQDVTLIILEVAAIISLGLSFYKPPDAEREHCGRAAGGVEDEGEAEAGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQRFTVLRGGQVIQIPVSEIVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVKKTLDKDPILLSGTHVMEGSGKMLVTAVGVNSQTGIIFMLLGGGDDDDDDDEEKKEEKERKKREKEEKRREKEEKKKEKERKKKEQKDKKKKKDGGTVEMEPLNSDDGTEEEQKKAKIPKKEKSVLQGKLTKLAVQIGKAGLFMSAITVLILVVLFLVDTFWIQGLPWIKDCTPIYIQFFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQVFIAGRHFKKVPEPDLIPGNIMNLLVTGIGVNCAYTSKIMSAEKEGGLPRQVGNKTECALLGFVTDLRKDYQAIRCEYPEEKLYKVYTFNSVRKSMSTVLKNSDGSYRMFSKGASEILLKKCCKILTSNGDAKHFRPTDRDDMVTQVIEPMASEGLRTICLAYRDFLVSDGEPDWNNEGDILTGLTCICVVGIEDPVRPEVPDAIKKCQRAGITVRMVTGDNINTARAIATKCGILHIGDDFLCLEGKEFNRRIRNELGEIEQERLDKVWPKLRVLARSSPTDKHTLVKGIIDSTVLEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAVYQLTIIFTLLFAGEQIFDIDSGRNAPLHAPPSEHYTVVFNTFVLMQLFNEINARKIHGERNVFEGIFNNIIFCSILFGTFIIQFVIVQFGGKPFSCVGLSVEQWLWCVFLGFGSLLWGQVISTVPTRRLKFLITMGHGTKKEEIPEDELEEMEDLDEIDHAEMELRRGQVLWCRGLNRIQTQIRVVNAFRDSMSPYDGLETPESRSSIHNFMSHPEFRIEDSEPAIPLIDDTDPEEDAPTKRNAAPSPLPLVLSSPNQNNNAIDGRGFHHHIDLAKTILPPTPGSPLHSLETSL

Target ORF information:

RefSeq Version	XM_009297908.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 1b (atp2b1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297908.2

ORF Insert Sequence:

ATGGCCAAAA ACTCATTCCG TGGGTCGAAG TACAAGCCAG AGGCCAATCA TGATGGACAT 
TTTGGCTGCA CACTCAAAGA GCTTCGTTCT CTTATGGAAC TCAGAGGAAC GGATGGATTA 
CAAAGAATTC AGGAGTGCTA TGGAGATGTT CAAGGACTCT GCTCCAAGTT AAAATCATCA 
CCGATAGAAG GCTTAAGTGG TCATCCCGAT GACATTGCAC GAAGAAAAGA AGAGTTCGGA 
AAAAATTTTA TACCTCCTAA AAAGCCAAAA ACATTCCTTC AATTAGTGTG GGAAGCACTG 
CAGGATGTGA CTCTAATTAT TTTGGAAGTT GCAGCCATAA TATCCTTAGG CCTTTCCTTT 
TATAAACCTC CCGATGCCGA GAGAGAACAC TGTGGTCGGG CGGCTGGAGG TGTCGAGGAT 
GAGGGGGAGG CAGAAGCGGG CTGGATTGAA GGTGCTGCCA TCCTCCTGTC TGTCGTCTGC 
GTGGTTCTTG TCACAGCTTT TAACGACTGG AGTAAAGAGA AACAGTTTCG TGGACTCCAA 
AGCCGGATTG AACAAGAGCA GAGGTTTACA GTACTCAGAG GCGGTCAAGT CATTCAGATA 
CCAGTGTCCG AGATTGTGGT CGGAGACATC GCACAAGTTA AATATGGTGA CCTCCTTCCT 
GCTGATGGTG TACTAATCCA AGGCAACGAT CTAAAAATTG ATGAGAGCTC CCTCACTGGA 
GAGTCGGATC ACGTCAAAAA AACTCTGGAC AAAGACCCCA TTCTTCTTTC AGGCACACAT 
GTAATGGAGG GCTCCGGAAA AATGCTGGTC ACTGCAGTGG GGGTTAATTC TCAAACGGGA 
ATTATTTTCA TGCTGCTCGG AGGTGGAGAT GACGATGACG ACGATGATGA GGAGAAAAAG 
GAAGAGAAGG AGAGGAAAAA GAGGGAGAAG GAAGAGAAGA GGAGAGAAAA GGAGGAGAAA 
AAGAAAGAGA AAGAGAGGAA GAAGAAGGAG CAGAAAGACA AAAAGAAAAA GAAAGATGGA 
GGTACAGTAG AAATGGAGCC CCTCAACAGT GATGATGGAA CAGAAGAAGA GCAGAAAAAA 
GCCAAGATAC CAAAGAAAGA AAAATCGGTC CTTCAAGGAA AGCTTACCAA ACTAGCTGTG 
CAAATTGGAA AAGCGGGACT CTTCATGTCG GCCATCACCG TCCTCATTCT TGTGGTGCTC 
TTTTTGGTGG ACACTTTCTG GATCCAAGGG CTTCCATGGA TCAAAGACTG CACTCCTATC 
TACATCCAGT TCTTTGTGAA GTTCTTTATT ATCGGCGTCA CTGTGTTGGT TGTTGCTGTG 
CCTGAAGGTT TACCTCTTGC CGTCACAATC TCATTGGCAT ATTCTGTGAA GAAAATGATG 
AAGGACAATA ACCTGGTGAG ACATCTGGAC GCTTGTGAGA CGATGGGCAA TGCTACCGCC 
ATTTGCTCGG ATAAAACGGG CACCCTAACT ATGAACAGGA TGACAGTGGT TCAGGTGTTT 
ATTGCAGGCA GACACTTCAA GAAGGTTCCA GAGCCCGATC TGATTCCTGG AAACATCATG 
AATCTGCTGG TCACAGGCAT AGGCGTTAAC TGTGCATACA CTTCAAAAAT CATGTCTGCT 
GAAAAAGAGG GTGGCCTGCC CCGTCAAGTT GGGAACAAAA CTGAATGTGC CTTGCTAGGT 
TTTGTCACTG ATTTACGTAA GGATTACCAA GCCATTCGCT GTGAATATCC TGAGGAAAAA 
CTGTACAAAG TGTACACCTT TAACTCAGTC AGGAAATCCA TGAGTACAGT CCTGAAAAAT 
TCTGATGGAA GTTACCGCAT GTTTAGCAAG GGAGCTTCAG AAATCCTCCT AAAGAAATGC 
TGCAAAATCC TGACAAGCAA TGGTGATGCC AAACATTTCA GACCCACTGA TCGAGATGAC 
ATGGTGACAC AGGTGATAGA GCCTATGGCC TCTGAGGGAT TGAGGACCAT CTGCCTTGCC 
TACAGGGACT TCCTTGTTTC GGATGGGGAG CCTGACTGGA ATAATGAGGG TGATATCCTA 
ACTGGACTGA CCTGCATCTG TGTGGTGGGC ATTGAGGATC CTGTAAGACC AGAGGTCCCT 
GATGCAATTA AAAAGTGTCA GCGAGCTGGG ATCACTGTGC GGATGGTCAC AGGGGATAAT 
ATTAACACTG CTCGAGCCAT AGCAACAAAG TGTGGCATTC TCCATATCGG GGATGACTTC 
CTGTGCCTTG AAGGCAAAGA ATTCAACAGG CGGATACGGA ATGAACTAGG AGAGATTGAG 
CAAGAACGAT TGGACAAGGT TTGGCCCAAA CTGCGGGTAC TTGCAAGATC CTCTCCTACT 
GATAAGCATA CGTTGGTTAA AGGTATAATC GATAGCACCG TCTTAGAACA GAGACAAGTA 
GTAGCTGTCA CTGGAGATGG AACCAATGAT GGTCCTGCGC TCAAAAAAGC TGATGTTGGC 
TTTGCGATGG GGATTGCAGG GACAGATGTT GCTAAAGAAG CATCAGACAT CATCCTCACC 
GATGACAACT TCAGCAGCAT CGTGAAAGCA GTAATGTGGG GGAGGAACGT TTACGACAGC 
ATTTCGAAGT TCCTGCAATT TCAGTTGACA GTCAATGTGG TGGCTGTCAT AGTGGCGTTT 
ACCGGAGCCT GTATCACACA GGACTCCCCT CTTAAAGCTG TACAGATGCT CTGGGTGAAT 
TTAATCATGG ACACATTTGC ATCACTAGCT CTAGCAACAG AACCTCCCAC TGAATCTTTA 
CTGTTGCGCA AACCCTATGG TCGCAATAAG CCCCTTATTT CCCGCACCAT GATGAAGAAC 
ATCCTTGGAC ATGCTGTATA TCAGCTGACC ATCATCTTCA CACTACTGTT TGCAGGAGAG 
CAGATCTTTG ATATTGACAG TGGCAGGAAC GCTCCTCTTC ACGCTCCTCC TTCAGAGCAC 
TACACCGTTG TCTTTAACAC TTTCGTGCTG ATGCAGCTCT TCAATGAGAT TAACGCTCGA 
AAGATCCATG GAGAGAGAAA TGTTTTCGAG GGTATCTTCA ATAACATAAT CTTCTGCAGC 
ATTTTATTTG GGACCTTTAT TATCCAGTTT GTAATTGTGC AGTTTGGAGG GAAGCCATTT 
AGCTGTGTTG GTCTCTCCGT TGAGCAGTGG CTGTGGTGCG TTTTCCTTGG TTTTGGATCT 
CTTCTATGGG GACAGGTGAT TTCCACCGTT CCCACAAGAC GACTGAAGTT CCTGATAACC 
ATGGGTCATG GCACTAAGAA GGAGGAGATC CCCGAGGACG AGCTGGAGGA GATGGAAGAC 
CTGGATGAAA TCGACCATGC AGAAATGGAG CTGAGAAGGG GGCAGGTTCT GTGGTGCAGG 
GGTCTCAACA GGATCCAGAC ACAGATCCGT GTGGTTAATG CTTTCCGGGA TTCCATGTCC 
CCATACGACG GCCTTGAGAC GCCTGAGTCT CGCAGCTCTA TTCACAATTT CATGAGTCAT 
CCGGAGTTTC GCATCGAGGA TTCAGAACCA GCGATTCCTC TAATCGACGA CACAGATCCT 
GAGGAGGACG CACCCACCAA ACGCAACGCA GCTCCTTCTC CACTGCCTCT AGTTTTATCT 
TCCCCCAATC AGAACAATAA CGCCATCGAT GGCAGGGGCT TCCATCACCA CATAGATCTA 
GCAAAAACTA TCCTTCCTCC CACTCCTGGA AGCCCATTAC ACAGCCTGGA GACATCGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b1b ( XM_009297908.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296183.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 1b (atp2b1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa43455
Clone ID Related Accession (Same CDS sequence)	XM_009297908.2 , XM_009297908.3 , XM_009297909.1
Accession Version	XM_009297908.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium-transporting ATPase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297908.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAA ACTCATTCCG TGGGTCGAAG TACAAGCCAG AGGCCAATCA TGATGGACAT 
TTTGGCTGCA CACTCAAAGA GCTTCGTTCT CTTATGGAAC TCAGAGGAAC GGATGGATTA 
CAAAGAATTC AGGAGTGCTA TGGAGATGTT CAAGGACTCT GCTCCAAGTT AAAATCATCA 
CCGATAGAAG GCTTAAGTGG TCATCCCGAT GACATTGCAC GAAGAAAAGA AGAGTTCGGA 
AAAAATTTTA TACCTCCTAA AAAGCCAAAA ACATTCCTTC AATTAGTGTG GGAAGCACTG 
CAGGATGTGA CTCTAATTAT TTTGGAAGTT GCAGCCATAA TATCCTTAGG CCTTTCCTTT 
TATAAACCTC CCGATGCCGA GAGAGAACAC TGTGGTCGGG CGGCTGGAGG TGTCGAGGAT 
GAGGGGGAGG CAGAAGCGGG CTGGATTGAA GGTGCTGCCA TCCTCCTGTC TGTCGTCTGC 
GTGGTTCTTG TCACAGCTTT TAACGACTGG AGTAAAGAGA AACAGTTTCG TGGACTCCAA 
AGCCGGATTG AACAAGAGCA GAGGTTTACA GTACTCAGAG GCGGTCAAGT CATTCAGATA 
CCAGTGTCCG AGATTGTGGT CGGAGACATC GCACAAGTTA AATATGGTGA CCTCCTTCCT 
GCTGATGGTG TACTAATCCA AGGCAACGAT CTAAAAATTG ATGAGAGCTC CCTCACTGGA 
GAGTCGGATC ACGTCAAAAA AACTCTGGAC AAAGACCCCA TTCTTCTTTC AGGCACACAT 
GTAATGGAGG GCTCCGGAAA AATGCTGGTC ACTGCAGTGG GGGTTAATTC TCAAACGGGA 
ATTATTTTCA TGCTGCTCGG AGGTGGAGAT GACGATGACG ACGATGATGA GGAGAAAAAG 
GAAGAGAAGG AGAGGAAAAA GAGGGAGAAG GAAGAGAAGA GGAGAGAAAA GGAGGAGAAA 
AAGAAAGAGA AAGAGAGGAA GAAGAAGGAG CAGAAAGACA AAAAGAAAAA GAAAGATGGA 
GGTACAGTAG AAATGGAGCC CCTCAACAGT GATGATGGAA CAGAAGAAGA GCAGAAAAAA 
GCCAAGATAC CAAAGAAAGA AAAATCGGTC CTTCAAGGAA AGCTTACCAA ACTAGCTGTG 
CAAATTGGAA AAGCGGGACT CTTCATGTCG GCCATCACCG TCCTCATTCT TGTGGTGCTC 
TTTTTGGTGG ACACTTTCTG GATCCAAGGG CTTCCATGGA TCAAAGACTG CACTCCTATC 
TACATCCAGT TCTTTGTGAA GTTCTTTATT ATCGGCGTCA CTGTGTTGGT TGTTGCTGTG 
CCTGAAGGTT TACCTCTTGC CGTCACAATC TCATTGGCAT ATTCTGTGAA GAAAATGATG 
AAGGACAATA ACCTGGTGAG ACATCTGGAC GCTTGTGAGA CGATGGGCAA TGCTACCGCC 
ATTTGCTCGG ATAAAACGGG CACCCTAACT ATGAACAGGA TGACAGTGGT TCAGGTGTTT 
ATTGCAGGCA GACACTTCAA GAAGGTTCCA GAGCCCGATC TGATTCCTGG AAACATCATG 
AATCTGCTGG TCACAGGCAT AGGCGTTAAC TGTGCATACA CTTCAAAAAT CATGTCTGCT 
GAAAAAGAGG GTGGCCTGCC CCGTCAAGTT GGGAACAAAA CTGAATGTGC CTTGCTAGGT 
TTTGTCACTG ATTTACGTAA GGATTACCAA GCCATTCGCT GTGAATATCC TGAGGAAAAA 
CTGTACAAAG TGTACACCTT TAACTCAGTC AGGAAATCCA TGAGTACAGT CCTGAAAAAT 
TCTGATGGAA GTTACCGCAT GTTTAGCAAG GGAGCTTCAG AAATCCTCCT AAAGAAATGC 
TGCAAAATCC TGACAAGCAA TGGTGATGCC AAACATTTCA GACCCACTGA TCGAGATGAC 
ATGGTGACAC AGGTGATAGA GCCTATGGCC TCTGAGGGAT TGAGGACCAT CTGCCTTGCC 
TACAGGGACT TCCTTGTTTC GGATGGGGAG CCTGACTGGA ATAATGAGGG TGATATCCTA 
ACTGGACTGA CCTGCATCTG TGTGGTGGGC ATTGAGGATC CTGTAAGACC AGAGGTCCCT 
GATGCAATTA AAAAGTGTCA GCGAGCTGGG ATCACTGTGC GGATGGTCAC AGGGGATAAT 
ATTAACACTG CTCGAGCCAT AGCAACAAAG TGTGGCATTC TCCATATCGG GGATGACTTC 
CTGTGCCTTG AAGGCAAAGA ATTCAACAGG CGGATACGGA ATGAACTAGG AGAGATTGAG 
CAAGAACGAT TGGACAAGGT TTGGCCCAAA CTGCGGGTAC TTGCAAGATC CTCTCCTACT 
GATAAGCATA CGTTGGTTAA AGGTATAATC GATAGCACCG TCTTAGAACA GAGACAAGTA 
GTAGCTGTCA CTGGAGATGG AACCAATGAT GGTCCTGCGC TCAAAAAAGC TGATGTTGGC 
TTTGCGATGG GGATTGCAGG GACAGATGTT GCTAAAGAAG CATCAGACAT CATCCTCACC 
GATGACAACT TCAGCAGCAT CGTGAAAGCA GTAATGTGGG GGAGGAACGT TTACGACAGC 
ATTTCGAAGT TCCTGCAATT TCAGTTGACA GTCAATGTGG TGGCTGTCAT AGTGGCGTTT 
ACCGGAGCCT GTATCACACA GGACTCCCCT CTTAAAGCTG TACAGATGCT CTGGGTGAAT 
TTAATCATGG ACACATTTGC ATCACTAGCT CTAGCAACAG AACCTCCCAC TGAATCTTTA 
CTGTTGCGCA AACCCTATGG TCGCAATAAG CCCCTTATTT CCCGCACCAT GATGAAGAAC 
ATCCTTGGAC ATGCTGTATA TCAGCTGACC ATCATCTTCA CACTACTGTT TGCAGGAGAG 
CAGATCTTTG ATATTGACAG TGGCAGGAAC GCTCCTCTTC ACGCTCCTCC TTCAGAGCAC 
TACACCGTTG TCTTTAACAC TTTCGTGCTG ATGCAGCTCT TCAATGAGAT TAACGCTCGA 
AAGATCCATG GAGAGAGAAA TGTTTTCGAG GGTATCTTCA ATAACATAAT CTTCTGCAGC 
ATTTTATTTG GGACCTTTAT TATCCAGTTT GTAATTGTGC AGTTTGGAGG GAAGCCATTT 
AGCTGTGTTG GTCTCTCCGT TGAGCAGTGG CTGTGGTGCG TTTTCCTTGG TTTTGGATCT 
CTTCTATGGG GACAGGTGAT TTCCACCGTT CCCACAAGAC GACTGAAGTT CCTGATAACC 
ATGGGTCATG GCACTAAGAA GGAGGAGATC CCCGAGGACG AGCTGGAGGA GATGGAAGAC 
CTGGATGAAA TCGACCATGC AGAAATGGAG CTGAGAAGGG GGCAGGTTCT GTGGTGCAGG 
GGTCTCAACA GGATCCAGAC ACAGATCCGT GTGGTTAATG CTTTCCGGGA TTCCATGTCC 
CCATACGACG GCCTTGAGAC GCCTGAGTCT CGCAGCTCTA TTCACAATTT CATGAGTCAT 
CCGGAGTTTC GCATCGAGGA TTCAGAACCA GCGATTCCTC TAATCGACGA CACAGATCCT 
GAGGAGGACG CACCCACCAA ACGCAACGCA GCTCCTTCTC CACTGCCTCT AGTTTTATCT 
TCCCCCAATC AGAACAATAA CGCCATCGAT GGCAGGGGCT TCCATCACCA CATAGATCTA 
GCAAAAACTA TCCTTCCTCC CACTCCTGGA AGCCCATTAC ACAGCCTGGA GACATCGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296183.1
CDS	454..4176
Translation	MAKNSFRGSKYKPEANHDGHFGCTLKELRSLMELRGTDGLQRIQECYGDVQGLCSKLKSSPIEGLSGHPDDIARRKEEFGKNFIPPKKPKTFLQLVWEALQDVTLIILEVAAIISLGLSFYKPPDAEREHCGRAAGGVEDEGEAEAGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQRFTVLRGGQVIQIPVSEIVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVKKTLDKDPILLSGTHVMEGSGKMLVTAVGVNSQTGIIFMLLGGGDDDDDDDEEKKEEKERKKREKEEKRREKEEKKKEKERKKKEQKDKKKKKDGGTVEMEPLNSDDGTEEEQKKAKIPKKEKSVLQGKLTKLAVQIGKAGLFMSAITVLILVVLFLVDTFWIQGLPWIKDCTPIYIQFFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQVFIAGRHFKKVPEPDLIPGNIMNLLVTGIGVNCAYTSKIMSAEKEGGLPRQVGNKTECALLGFVTDLRKDYQAIRCEYPEEKLYKVYTFNSVRKSMSTVLKNSDGSYRMFSKGASEILLKKCCKILTSNGDAKHFRPTDRDDMVTQVIEPMASEGLRTICLAYRDFLVSDGEPDWNNEGDILTGLTCICVVGIEDPVRPEVPDAIKKCQRAGITVRMVTGDNINTARAIATKCGILHIGDDFLCLEGKEFNRRIRNELGEIEQERLDKVWPKLRVLARSSPTDKHTLVKGIIDSTVLEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAVYQLTIIFTLLFAGEQIFDIDSGRNAPLHAPPSEHYTVVFNTFVLMQLFNEINARKIHGERNVFEGIFNNIIFCSILFGTFIIQFVIVQFGGKPFSCVGLSVEQWLWCVFLGFGSLLWGQVISTVPTRRLKFLITMGHGTKKEEIPEDELEEMEDLDEIDHAEMELRRGQVLWCRGLNRIQTQIRVVNAFRDSMSPYDGLETPESRSSIHNFMSHPEFRIEDSEPAIPLIDDTDPEEDAPTKRNAAPSPLPLVLSSPNQNNNAIDGRGFHHHIDLAKTILPPTPGSPLHSLETSL

Target ORF information:

RefSeq Version	XM_009297908.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 1b (atp2b1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297908.3

ORF Insert Sequence:

ATGGCCAAAA ACTCATTCCG TGGGTCGAAG TACAAGCCAG AGGCCAATCA TGATGGACAT 
TTTGGCTGCA CACTCAAAGA GCTTCGTTCT CTTATGGAAC TCAGAGGAAC GGATGGATTA 
CAAAGAATTC AGGAGTGCTA TGGAGATGTT CAAGGACTCT GCTCCAAGTT AAAATCATCA 
CCGATAGAAG GCTTAAGTGG TCATCCCGAT GACATTGCAC GAAGAAAAGA AGAGTTCGGA 
AAAAATTTTA TACCTCCTAA AAAGCCAAAA ACATTCCTTC AATTAGTGTG GGAAGCACTG 
CAGGATGTGA CTCTAATTAT TTTGGAAGTT GCAGCCATAA TATCCTTAGG CCTTTCCTTT 
TATAAACCTC CCGATGCCGA GAGAGAACAC TGTGGTCGGG CGGCTGGAGG TGTCGAGGAT 
GAGGGGGAGG CAGAAGCGGG CTGGATTGAA GGTGCTGCCA TCCTCCTGTC TGTCGTCTGC 
GTGGTTCTTG TCACAGCTTT TAACGACTGG AGTAAAGAGA AACAGTTTCG TGGACTCCAA 
AGCCGGATTG AACAAGAGCA GAGGTTTACA GTACTCAGAG GCGGTCAAGT CATTCAGATA 
CCAGTGTCCG AGATTGTGGT CGGAGACATC GCACAAGTTA AATATGGTGA CCTCCTTCCT 
GCTGATGGTG TACTAATCCA AGGCAACGAT CTAAAAATTG ATGAGAGCTC CCTCACTGGA 
GAGTCGGATC ACGTCAAAAA AACTCTGGAC AAAGACCCCA TTCTTCTTTC AGGCACACAT 
GTAATGGAGG GCTCCGGAAA AATGCTGGTC ACTGCAGTGG GGGTTAATTC TCAAACGGGA 
ATTATTTTCA TGCTGCTCGG AGGTGGAGAT GACGATGACG ACGATGATGA GGAGAAAAAG 
GAAGAGAAGG AGAGGAAAAA GAGGGAGAAG GAAGAGAAGA GGAGAGAAAA GGAGGAGAAA 
AAGAAAGAGA AAGAGAGGAA GAAGAAGGAG CAGAAAGACA AAAAGAAAAA GAAAGATGGA 
GGTACAGTAG AAATGGAGCC CCTCAACAGT GATGATGGAA CAGAAGAAGA GCAGAAAAAA 
GCCAAGATAC CAAAGAAAGA AAAATCGGTC CTTCAAGGAA AGCTTACCAA ACTAGCTGTG 
CAAATTGGAA AAGCGGGACT CTTCATGTCG GCCATCACCG TCCTCATTCT TGTGGTGCTC 
TTTTTGGTGG ACACTTTCTG GATCCAAGGG CTTCCATGGA TCAAAGACTG CACTCCTATC 
TACATCCAGT TCTTTGTGAA GTTCTTTATT ATCGGCGTCA CTGTGTTGGT TGTTGCTGTG 
CCTGAAGGTT TACCTCTTGC CGTCACAATC TCATTGGCAT ATTCTGTGAA GAAAATGATG 
AAGGACAATA ACCTGGTGAG ACATCTGGAC GCTTGTGAGA CGATGGGCAA TGCTACCGCC 
ATTTGCTCGG ATAAAACGGG CACCCTAACT ATGAACAGGA TGACAGTGGT TCAGGTGTTT 
ATTGCAGGCA GACACTTCAA GAAGGTTCCA GAGCCCGATC TGATTCCTGG AAACATCATG 
AATCTGCTGG TCACAGGCAT AGGCGTTAAC TGTGCATACA CTTCAAAAAT CATGTCTGCT 
GAAAAAGAGG GTGGCCTGCC CCGTCAAGTT GGGAACAAAA CTGAATGTGC CTTGCTAGGT 
TTTGTCACTG ATTTACGTAA GGATTACCAA GCCATTCGCT GTGAATATCC TGAGGAAAAA 
CTGTACAAAG TGTACACCTT TAACTCAGTC AGGAAATCCA TGAGTACAGT CCTGAAAAAT 
TCTGATGGAA GTTACCGCAT GTTTAGCAAG GGAGCTTCAG AAATCCTCCT AAAGAAATGC 
TGCAAAATCC TGACAAGCAA TGGTGATGCC AAACATTTCA GACCCACTGA TCGAGATGAC 
ATGGTGACAC AGGTGATAGA GCCTATGGCC TCTGAGGGAT TGAGGACCAT CTGCCTTGCC 
TACAGGGACT TCCTTGTTTC GGATGGGGAG CCTGACTGGA ATAATGAGGG TGATATCCTA 
ACTGGACTGA CCTGCATCTG TGTGGTGGGC ATTGAGGATC CTGTAAGACC AGAGGTCCCT 
GATGCAATTA AAAAGTGTCA GCGAGCTGGG ATCACTGTGC GGATGGTCAC AGGGGATAAT 
ATTAACACTG CTCGAGCCAT AGCAACAAAG TGTGGCATTC TCCATATCGG GGATGACTTC 
CTGTGCCTTG AAGGCAAAGA ATTCAACAGG CGGATACGGA ATGAACTAGG AGAGATTGAG 
CAAGAACGAT TGGACAAGGT TTGGCCCAAA CTGCGGGTAC TTGCAAGATC CTCTCCTACT 
GATAAGCATA CGTTGGTTAA AGGTATAATC GATAGCACCG TCTTAGAACA GAGACAAGTA 
GTAGCTGTCA CTGGAGATGG AACCAATGAT GGTCCTGCGC TCAAAAAAGC TGATGTTGGC 
TTTGCGATGG GGATTGCAGG GACAGATGTT GCTAAAGAAG CATCAGACAT CATCCTCACC 
GATGACAACT TCAGCAGCAT CGTGAAAGCA GTAATGTGGG GGAGGAACGT TTACGACAGC 
ATTTCGAAGT TCCTGCAATT TCAGTTGACA GTCAATGTGG TGGCTGTCAT AGTGGCGTTT 
ACCGGAGCCT GTATCACACA GGACTCCCCT CTTAAAGCTG TACAGATGCT CTGGGTGAAT 
TTAATCATGG ACACATTTGC ATCACTAGCT CTAGCAACAG AACCTCCCAC TGAATCTTTA 
CTGTTGCGCA AACCCTATGG TCGCAATAAG CCCCTTATTT CCCGCACCAT GATGAAGAAC 
ATCCTTGGAC ATGCTGTATA TCAGCTGACC ATCATCTTCA CACTACTGTT TGCAGGAGAG 
CAGATCTTTG ATATTGACAG TGGCAGGAAC GCTCCTCTTC ACGCTCCTCC TTCAGAGCAC 
TACACCGTTG TCTTTAACAC TTTCGTGCTG ATGCAGCTCT TCAATGAGAT TAACGCTCGA 
AAGATCCATG GAGAGAGAAA TGTTTTCGAG GGTATCTTCA ATAACATAAT CTTCTGCAGC 
ATTTTATTTG GGACCTTTAT TATCCAGTTT GTAATTGTGC AGTTTGGAGG GAAGCCATTT 
AGCTGTGTTG GTCTCTCCGT TGAGCAGTGG CTGTGGTGCG TTTTCCTTGG TTTTGGATCT 
CTTCTATGGG GACAGGTGAT TTCCACCGTT CCCACAAGAC GACTGAAGTT CCTGATAACC 
ATGGGTCATG GCACTAAGAA GGAGGAGATC CCCGAGGACG AGCTGGAGGA GATGGAAGAC 
CTGGATGAAA TCGACCATGC AGAAATGGAG CTGAGAAGGG GGCAGGTTCT GTGGTGCAGG 
GGTCTCAACA GGATCCAGAC ACAGATCCGT GTGGTTAATG CTTTCCGGGA TTCCATGTCC 
CCATACGACG GCCTTGAGAC GCCTGAGTCT CGCAGCTCTA TTCACAATTT CATGAGTCAT 
CCGGAGTTTC GCATCGAGGA TTCAGAACCA GCGATTCCTC TAATCGACGA CACAGATCCT 
GAGGAGGACG CACCCACCAA ACGCAACGCA GCTCCTTCTC CACTGCCTCT AGTTTTATCT 
TCCCCCAATC AGAACAATAA CGCCATCGAT GGCAGGGGCT TCCATCACCA CATAGATCTA 
GCAAAAACTA TCCTTCCTCC CACTCCTGGA AGCCCATTAC ACAGCCTGGA GACATCGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b1b ( XM_009297909.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296184.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 1b (atp2b1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43455
Clone ID Related Accession (Same CDS sequence)	XM_009297908.2 , XM_009297908.3 , XM_009297909.1
Accession Version	XM_009297909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium-transporting ATPase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAA ACTCATTCCG TGGGTCGAAG TACAAGCCAG AGGCCAATCA TGATGGACAT 
TTTGGCTGCA CACTCAAAGA GCTTCGTTCT CTTATGGAAC TCAGAGGAAC GGATGGATTA 
CAAAGAATTC AGGAGTGCTA TGGAGATGTT CAAGGACTCT GCTCCAAGTT AAAATCATCA 
CCGATAGAAG GCTTAAGTGG TCATCCCGAT GACATTGCAC GAAGAAAAGA AGAGTTCGGA 
AAAAATTTTA TACCTCCTAA AAAGCCAAAA ACATTCCTTC AATTAGTGTG GGAAGCACTG 
CAGGATGTGA CTCTAATTAT TTTGGAAGTT GCAGCCATAA TATCCTTAGG CCTTTCCTTT 
TATAAACCTC CCGATGCCGA GAGAGAACAC TGTGGTCGGG CGGCTGGAGG TGTCGAGGAT 
GAGGGGGAGG CAGAAGCGGG CTGGATTGAA GGTGCTGCCA TCCTCCTGTC TGTCGTCTGC 
GTGGTTCTTG TCACAGCTTT TAACGACTGG AGTAAAGAGA AACAGTTTCG TGGACTCCAA 
AGCCGGATTG AACAAGAGCA GAGGTTTACA GTACTCAGAG GCGGTCAAGT CATTCAGATA 
CCAGTGTCCG AGATTGTGGT CGGAGACATC GCACAAGTTA AATATGGTGA CCTCCTTCCT 
GCTGATGGTG TACTAATCCA AGGCAACGAT CTAAAAATTG ATGAGAGCTC CCTCACTGGA 
GAGTCGGATC ACGTCAAAAA AACTCTGGAC AAAGACCCCA TTCTTCTTTC AGGCACACAT 
GTAATGGAGG GCTCCGGAAA AATGCTGGTC ACTGCAGTGG GGGTTAATTC TCAAACGGGA 
ATTATTTTCA TGCTGCTCGG AGGTGGAGAT GACGATGACG ACGATGATGA GGAGAAAAAG 
GAAGAGAAGG AGAGGAAAAA GAGGGAGAAG GAAGAGAAGA GGAGAGAAAA GGAGGAGAAA 
AAGAAAGAGA AAGAGAGGAA GAAGAAGGAG CAGAAAGACA AAAAGAAAAA GAAAGATGGA 
GGTACAGTAG AAATGGAGCC CCTCAACAGT GATGATGGAA CAGAAGAAGA GCAGAAAAAA 
GCCAAGATAC CAAAGAAAGA AAAATCGGTC CTTCAAGGAA AGCTTACCAA ACTAGCTGTG 
CAAATTGGAA AAGCGGGACT CTTCATGTCG GCCATCACCG TCCTCATTCT TGTGGTGCTC 
TTTTTGGTGG ACACTTTCTG GATCCAAGGG CTTCCATGGA TCAAAGACTG CACTCCTATC 
TACATCCAGT TCTTTGTGAA GTTCTTTATT ATCGGCGTCA CTGTGTTGGT TGTTGCTGTG 
CCTGAAGGTT TACCTCTTGC CGTCACAATC TCATTGGCAT ATTCTGTGAA GAAAATGATG 
AAGGACAATA ACCTGGTGAG ACATCTGGAC GCTTGTGAGA CGATGGGCAA TGCTACCGCC 
ATTTGCTCGG ATAAAACGGG CACCCTAACT ATGAACAGGA TGACAGTGGT TCAGGTGTTT 
ATTGCAGGCA GACACTTCAA GAAGGTTCCA GAGCCCGATC TGATTCCTGG AAACATCATG 
AATCTGCTGG TCACAGGCAT AGGCGTTAAC TGTGCATACA CTTCAAAAAT CATGTCTGCT 
GAAAAAGAGG GTGGCCTGCC CCGTCAAGTT GGGAACAAAA CTGAATGTGC CTTGCTAGGT 
TTTGTCACTG ATTTACGTAA GGATTACCAA GCCATTCGCT GTGAATATCC TGAGGAAAAA 
CTGTACAAAG TGTACACCTT TAACTCAGTC AGGAAATCCA TGAGTACAGT CCTGAAAAAT 
TCTGATGGAA GTTACCGCAT GTTTAGCAAG GGAGCTTCAG AAATCCTCCT AAAGAAATGC 
TGCAAAATCC TGACAAGCAA TGGTGATGCC AAACATTTCA GACCCACTGA TCGAGATGAC 
ATGGTGACAC AGGTGATAGA GCCTATGGCC TCTGAGGGAT TGAGGACCAT CTGCCTTGCC 
TACAGGGACT TCCTTGTTTC GGATGGGGAG CCTGACTGGA ATAATGAGGG TGATATCCTA 
ACTGGACTGA CCTGCATCTG TGTGGTGGGC ATTGAGGATC CTGTAAGACC AGAGGTCCCT 
GATGCAATTA AAAAGTGTCA GCGAGCTGGG ATCACTGTGC GGATGGTCAC AGGGGATAAT 
ATTAACACTG CTCGAGCCAT AGCAACAAAG TGTGGCATTC TCCATATCGG GGATGACTTC 
CTGTGCCTTG AAGGCAAAGA ATTCAACAGG CGGATACGGA ATGAACTAGG AGAGATTGAG 
CAAGAACGAT TGGACAAGGT TTGGCCCAAA CTGCGGGTAC TTGCAAGATC CTCTCCTACT 
GATAAGCATA CGTTGGTTAA AGGTATAATC GATAGCACCG TCTTAGAACA GAGACAAGTA 
GTAGCTGTCA CTGGAGATGG AACCAATGAT GGTCCTGCGC TCAAAAAAGC TGATGTTGGC 
TTTGCGATGG GGATTGCAGG GACAGATGTT GCTAAAGAAG CATCAGACAT CATCCTCACC 
GATGACAACT TCAGCAGCAT CGTGAAAGCA GTAATGTGGG GGAGGAACGT TTACGACAGC 
ATTTCGAAGT TCCTGCAATT TCAGTTGACA GTCAATGTGG TGGCTGTCAT AGTGGCGTTT 
ACCGGAGCCT GTATCACACA GGACTCCCCT CTTAAAGCTG TACAGATGCT CTGGGTGAAT 
TTAATCATGG ACACATTTGC ATCACTAGCT CTAGCAACAG AACCTCCCAC TGAATCTTTA 
CTGTTGCGCA AACCCTATGG TCGCAATAAG CCCCTTATTT CCCGCACCAT GATGAAGAAC 
ATCCTTGGAC ATGCTGTATA TCAGCTGACC ATCATCTTCA CACTACTGTT TGCAGGAGAG 
CAGATCTTTG ATATTGACAG TGGCAGGAAC GCTCCTCTTC ACGCTCCTCC TTCAGAGCAC 
TACACCGTTG TCTTTAACAC TTTCGTGCTG ATGCAGCTCT TCAATGAGAT TAACGCTCGA 
AAGATCCATG GAGAGAGAAA TGTTTTCGAG GGTATCTTCA ATAACATAAT CTTCTGCAGC 
ATTTTATTTG GGACCTTTAT TATCCAGTTT GTAATTGTGC AGTTTGGAGG GAAGCCATTT 
AGCTGTGTTG GTCTCTCCGT TGAGCAGTGG CTGTGGTGCG TTTTCCTTGG TTTTGGATCT 
CTTCTATGGG GACAGGTGAT TTCCACCGTT CCCACAAGAC GACTGAAGTT CCTGATAACC 
ATGGGTCATG GCACTAAGAA GGAGGAGATC CCCGAGGACG AGCTGGAGGA GATGGAAGAC 
CTGGATGAAA TCGACCATGC AGAAATGGAG CTGAGAAGGG GGCAGGTTCT GTGGTGCAGG 
GGTCTCAACA GGATCCAGAC ACAGATCCGT GTGGTTAATG CTTTCCGGGA TTCCATGTCC 
CCATACGACG GCCTTGAGAC GCCTGAGTCT CGCAGCTCTA TTCACAATTT CATGAGTCAT 
CCGGAGTTTC GCATCGAGGA TTCAGAACCA GCGATTCCTC TAATCGACGA CACAGATCCT 
GAGGAGGACG CACCCACCAA ACGCAACGCA GCTCCTTCTC CACTGCCTCT AGTTTTATCT 
TCCCCCAATC AGAACAATAA CGCCATCGAT GGCAGGGGCT TCCATCACCA CATAGATCTA 
GCAAAAACTA TCCTTCCTCC CACTCCTGGA AGCCCATTAC ACAGCCTGGA GACATCGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296184.1
CDS	412..4134
Translation	MAKNSFRGSKYKPEANHDGHFGCTLKELRSLMELRGTDGLQRIQECYGDVQGLCSKLKSSPIEGLSGHPDDIARRKEEFGKNFIPPKKPKTFLQLVWEALQDVTLIILEVAAIISLGLSFYKPPDAEREHCGRAAGGVEDEGEAEAGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQRFTVLRGGQVIQIPVSEIVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVKKTLDKDPILLSGTHVMEGSGKMLVTAVGVNSQTGIIFMLLGGGDDDDDDDEEKKEEKERKKREKEEKRREKEEKKKEKERKKKEQKDKKKKKDGGTVEMEPLNSDDGTEEEQKKAKIPKKEKSVLQGKLTKLAVQIGKAGLFMSAITVLILVVLFLVDTFWIQGLPWIKDCTPIYIQFFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQVFIAGRHFKKVPEPDLIPGNIMNLLVTGIGVNCAYTSKIMSAEKEGGLPRQVGNKTECALLGFVTDLRKDYQAIRCEYPEEKLYKVYTFNSVRKSMSTVLKNSDGSYRMFSKGASEILLKKCCKILTSNGDAKHFRPTDRDDMVTQVIEPMASEGLRTICLAYRDFLVSDGEPDWNNEGDILTGLTCICVVGIEDPVRPEVPDAIKKCQRAGITVRMVTGDNINTARAIATKCGILHIGDDFLCLEGKEFNRRIRNELGEIEQERLDKVWPKLRVLARSSPTDKHTLVKGIIDSTVLEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAVYQLTIIFTLLFAGEQIFDIDSGRNAPLHAPPSEHYTVVFNTFVLMQLFNEINARKIHGERNVFEGIFNNIIFCSILFGTFIIQFVIVQFGGKPFSCVGLSVEQWLWCVFLGFGSLLWGQVISTVPTRRLKFLITMGHGTKKEEIPEDELEEMEDLDEIDHAEMELRRGQVLWCRGLNRIQTQIRVVNAFRDSMSPYDGLETPESRSSIHNFMSHPEFRIEDSEPAIPLIDDTDPEEDAPTKRNAAPSPLPLVLSSPNQNNNAIDGRGFHHHIDLAKTILPPTPGSPLHSLETSL

Target ORF information:

RefSeq Version	XM_009297909.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 1b (atp2b1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297909.1

ORF Insert Sequence:

ATGGCCAAAA ACTCATTCCG TGGGTCGAAG TACAAGCCAG AGGCCAATCA TGATGGACAT 
TTTGGCTGCA CACTCAAAGA GCTTCGTTCT CTTATGGAAC TCAGAGGAAC GGATGGATTA 
CAAAGAATTC AGGAGTGCTA TGGAGATGTT CAAGGACTCT GCTCCAAGTT AAAATCATCA 
CCGATAGAAG GCTTAAGTGG TCATCCCGAT GACATTGCAC GAAGAAAAGA AGAGTTCGGA 
AAAAATTTTA TACCTCCTAA AAAGCCAAAA ACATTCCTTC AATTAGTGTG GGAAGCACTG 
CAGGATGTGA CTCTAATTAT TTTGGAAGTT GCAGCCATAA TATCCTTAGG CCTTTCCTTT 
TATAAACCTC CCGATGCCGA GAGAGAACAC TGTGGTCGGG CGGCTGGAGG TGTCGAGGAT 
GAGGGGGAGG CAGAAGCGGG CTGGATTGAA GGTGCTGCCA TCCTCCTGTC TGTCGTCTGC 
GTGGTTCTTG TCACAGCTTT TAACGACTGG AGTAAAGAGA AACAGTTTCG TGGACTCCAA 
AGCCGGATTG AACAAGAGCA GAGGTTTACA GTACTCAGAG GCGGTCAAGT CATTCAGATA 
CCAGTGTCCG AGATTGTGGT CGGAGACATC GCACAAGTTA AATATGGTGA CCTCCTTCCT 
GCTGATGGTG TACTAATCCA AGGCAACGAT CTAAAAATTG ATGAGAGCTC CCTCACTGGA 
GAGTCGGATC ACGTCAAAAA AACTCTGGAC AAAGACCCCA TTCTTCTTTC AGGCACACAT 
GTAATGGAGG GCTCCGGAAA AATGCTGGTC ACTGCAGTGG GGGTTAATTC TCAAACGGGA 
ATTATTTTCA TGCTGCTCGG AGGTGGAGAT GACGATGACG ACGATGATGA GGAGAAAAAG 
GAAGAGAAGG AGAGGAAAAA GAGGGAGAAG GAAGAGAAGA GGAGAGAAAA GGAGGAGAAA 
AAGAAAGAGA AAGAGAGGAA GAAGAAGGAG CAGAAAGACA AAAAGAAAAA GAAAGATGGA 
GGTACAGTAG AAATGGAGCC CCTCAACAGT GATGATGGAA CAGAAGAAGA GCAGAAAAAA 
GCCAAGATAC CAAAGAAAGA AAAATCGGTC CTTCAAGGAA AGCTTACCAA ACTAGCTGTG 
CAAATTGGAA AAGCGGGACT CTTCATGTCG GCCATCACCG TCCTCATTCT TGTGGTGCTC 
TTTTTGGTGG ACACTTTCTG GATCCAAGGG CTTCCATGGA TCAAAGACTG CACTCCTATC 
TACATCCAGT TCTTTGTGAA GTTCTTTATT ATCGGCGTCA CTGTGTTGGT TGTTGCTGTG 
CCTGAAGGTT TACCTCTTGC CGTCACAATC TCATTGGCAT ATTCTGTGAA GAAAATGATG 
AAGGACAATA ACCTGGTGAG ACATCTGGAC GCTTGTGAGA CGATGGGCAA TGCTACCGCC 
ATTTGCTCGG ATAAAACGGG CACCCTAACT ATGAACAGGA TGACAGTGGT TCAGGTGTTT 
ATTGCAGGCA GACACTTCAA GAAGGTTCCA GAGCCCGATC TGATTCCTGG AAACATCATG 
AATCTGCTGG TCACAGGCAT AGGCGTTAAC TGTGCATACA CTTCAAAAAT CATGTCTGCT 
GAAAAAGAGG GTGGCCTGCC CCGTCAAGTT GGGAACAAAA CTGAATGTGC CTTGCTAGGT 
TTTGTCACTG ATTTACGTAA GGATTACCAA GCCATTCGCT GTGAATATCC TGAGGAAAAA 
CTGTACAAAG TGTACACCTT TAACTCAGTC AGGAAATCCA TGAGTACAGT CCTGAAAAAT 
TCTGATGGAA GTTACCGCAT GTTTAGCAAG GGAGCTTCAG AAATCCTCCT AAAGAAATGC 
TGCAAAATCC TGACAAGCAA TGGTGATGCC AAACATTTCA GACCCACTGA TCGAGATGAC 
ATGGTGACAC AGGTGATAGA GCCTATGGCC TCTGAGGGAT TGAGGACCAT CTGCCTTGCC 
TACAGGGACT TCCTTGTTTC GGATGGGGAG CCTGACTGGA ATAATGAGGG TGATATCCTA 
ACTGGACTGA CCTGCATCTG TGTGGTGGGC ATTGAGGATC CTGTAAGACC AGAGGTCCCT 
GATGCAATTA AAAAGTGTCA GCGAGCTGGG ATCACTGTGC GGATGGTCAC AGGGGATAAT 
ATTAACACTG CTCGAGCCAT AGCAACAAAG TGTGGCATTC TCCATATCGG GGATGACTTC 
CTGTGCCTTG AAGGCAAAGA ATTCAACAGG CGGATACGGA ATGAACTAGG AGAGATTGAG 
CAAGAACGAT TGGACAAGGT TTGGCCCAAA CTGCGGGTAC TTGCAAGATC CTCTCCTACT 
GATAAGCATA CGTTGGTTAA AGGTATAATC GATAGCACCG TCTTAGAACA GAGACAAGTA 
GTAGCTGTCA CTGGAGATGG AACCAATGAT GGTCCTGCGC TCAAAAAAGC TGATGTTGGC 
TTTGCGATGG GGATTGCAGG GACAGATGTT GCTAAAGAAG CATCAGACAT CATCCTCACC 
GATGACAACT TCAGCAGCAT CGTGAAAGCA GTAATGTGGG GGAGGAACGT TTACGACAGC 
ATTTCGAAGT TCCTGCAATT TCAGTTGACA GTCAATGTGG TGGCTGTCAT AGTGGCGTTT 
ACCGGAGCCT GTATCACACA GGACTCCCCT CTTAAAGCTG TACAGATGCT CTGGGTGAAT 
TTAATCATGG ACACATTTGC ATCACTAGCT CTAGCAACAG AACCTCCCAC TGAATCTTTA 
CTGTTGCGCA AACCCTATGG TCGCAATAAG CCCCTTATTT CCCGCACCAT GATGAAGAAC 
ATCCTTGGAC ATGCTGTATA TCAGCTGACC ATCATCTTCA CACTACTGTT TGCAGGAGAG 
CAGATCTTTG ATATTGACAG TGGCAGGAAC GCTCCTCTTC ACGCTCCTCC TTCAGAGCAC 
TACACCGTTG TCTTTAACAC TTTCGTGCTG ATGCAGCTCT TCAATGAGAT TAACGCTCGA 
AAGATCCATG GAGAGAGAAA TGTTTTCGAG GGTATCTTCA ATAACATAAT CTTCTGCAGC 
ATTTTATTTG GGACCTTTAT TATCCAGTTT GTAATTGTGC AGTTTGGAGG GAAGCCATTT 
AGCTGTGTTG GTCTCTCCGT TGAGCAGTGG CTGTGGTGCG TTTTCCTTGG TTTTGGATCT 
CTTCTATGGG GACAGGTGAT TTCCACCGTT CCCACAAGAC GACTGAAGTT CCTGATAACC 
ATGGGTCATG GCACTAAGAA GGAGGAGATC CCCGAGGACG AGCTGGAGGA GATGGAAGAC 
CTGGATGAAA TCGACCATGC AGAAATGGAG CTGAGAAGGG GGCAGGTTCT GTGGTGCAGG 
GGTCTCAACA GGATCCAGAC ACAGATCCGT GTGGTTAATG CTTTCCGGGA TTCCATGTCC 
CCATACGACG GCCTTGAGAC GCCTGAGTCT CGCAGCTCTA TTCACAATTT CATGAGTCAT 
CCGGAGTTTC GCATCGAGGA TTCAGAACCA GCGATTCCTC TAATCGACGA CACAGATCCT 
GAGGAGGACG CACCCACCAA ACGCAACGCA GCTCCTTCTC CACTGCCTCT AGTTTTATCT 
TCCCCCAATC AGAACAATAA CGCCATCGAT GGCAGGGGCT TCCATCACCA CATAGATCTA 
GCAAAAACTA TCCTTCCTCC CACTCCTGGA AGCCCATTAC ACAGCCTGGA GACATCGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Epigenetic regulation of hematopoiesis by DNA methylation.
eLife5e11813(2016 Jan)
Gore AV,Athans B,Iben JR,Johnson K,Russanova V,Castranova D,Pham VN,Butler MG,Williams-Simons L,Nichols JT,Bresciani E,Feldman B,Kimmel CB,Liu PP,Weinstein BM

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The vertebrate ancestral repertoire of visual opsins, transducin alpha subunits and oxytocin/vasopressin receptors was established by duplication of their shared genomic region in the two rounds of early vertebrate genome duplications.
BMC evolutionary biology13238(2013 Nov)
Lagman D,Ocampo Daza D,Widmark J,Abalo XM,Sundström G,Larhammar D

Effects of dietary methylmercury on the zebrafish brain: histological, mitochondrial, and gene transcription analyses.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine25(1)165-80(2012 Feb)
Cambier S,Gonzalez P,Mesmer-Dudons N,Brèthes D,Fujimura M,Bourdineaud JP

Expression and water calcium dependence of calcium transporter isoforms in zebrafish gill mitochondrion-rich cells.
BMC genomics8354(2007 Oct)
Liao BK,Deng AN,Chen SC,Chou MY,Hwang PP

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atp2b1b cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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