ORF » Species Summary » Danio rerio » dysf cDNA ORF clone

dysf cDNA ORF clone, Danio rerio(zebrafish)

The following dysf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dysf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa35448 NM_001257193.1
Latest version!		 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa12912 XM_005172291.4
Latest version!		 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa12914 XM_005172293.4
Latest version!		 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa143308 XM_021477555.1
Latest version!		 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa143309 XM_021477556.1
Latest version!		 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa143310 XM_021477557.1
Latest version!		 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa143311 XM_021477558.1
Latest version!		 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa143312 XM_021477560.1
Latest version!		 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa43390 XM_021477559.1
Latest version!		 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa59639 XM_017356879.2
Latest version!		 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
View Old Accession Versions >>
ODa12914 XM_005172293.3 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa12912 XM_005172291.3 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa59639 XM_017356879.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    dysf ( NM_001257193.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001244122.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), mRNA.
    Price & Availability↑
    CloneID ODa35448
    Clone ID Related Accession (Same CDS sequence) NM_001257193.1
    Accession Version NM_001257193.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1561132800000
    Organism Danio rerio(zebrafish)
    Product dysferlin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FR725960.1. On Mar 18, 2012 this sequence version replaced XM_684324.4. ##Evidence-Data-START## Transcript exon combination :: FR725960.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAGAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG GTAGCCTTTT TCCGACTTGT GGAAAAAATA 
    TTCAATAATT TTGAAAAAAG CTTATTAATT GTGGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CGAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GGTGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGGTAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG 
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT 
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG 
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG 
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG 
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC 
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT 
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT 
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT 
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC 
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG 
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC 
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG 
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA 
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA 
    AACAGTCCGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC 
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA 
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT 
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT 
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG 
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA 
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG 
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG 
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG 
    GGGTACACAG ATGTGAATGG AGAAAAGGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT 
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGC GAGGATCTGA AGCGTGCAGT GGATGATCAA 
    GGGTGGGAAT ATGGAATAAC CCTCCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT 
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG 
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA 
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT 
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CAGGGCCGGC GGCCATCTTT 
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT 
    ACAACGTTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT 
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC 
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTG CATCAGAGTC AGAAGACGGT GACTGTTCGA 
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA 
    GACCATCTTG TGACTGAGAG GAGTCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC 
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC 
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT 
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA 
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG 
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA 
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA 
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT 
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG 
    AACCCTAATT TTGACGTCAA CATCCTGTCC ATTGATGTCC GATTGCCTAA GGAAGAGCTC 
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG 
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG 
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG 
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGC AGGATGAATT TATGGACTGG 
    TGGAGCAAGT TTTATGCCTC CATGGGTGAA AGGACCAAGT GTGGGAGTTA TCTGGAGAAA 
    GGGTTCGATA TGCTGCAGGT GTATGACAGA GAACTGGAGA AGACTGAAGG CTTTGAGCAA 
    TTTGCTGATT TCTGTCAAAC GTTTAAACTT TACCGTGGAA GAACCCAGAA TGACAGCGAA 
    GATCCTTCCG TTGTTGGAGA GTTCAAGGGT TCGTTTAAGA TCTATCCATT GCCTGATGAT 
    CCCCCTATGC CTGTACCCAC ACGTCAGTTT CGCAAACTCC CCCCGAACGG TATAGAGGAG 
    TGCTTGGTAC GCATCTACAT CATCCAGGCT TTTGGGTTAC AGCCCAAAGA TGCCAATGGC 
    AAGTGTGACC CCTACGTTAA GATCACGCTT GGCAAGAAAT CAGTCGATGA CCACGACAAT 
    TACATACCCT GTACTTTGGA GCCCGTCTTT GGAAAGATGT TTGAGCTGAC CTGCTGTCTG 
    CCTCTTGAGA AAGACCTGAA GATTACACTC TATGATTACG ACATGGTGAC TAAAGATGAG 
    AAAATCGGAG AGACCATCAT TGATTTGGAG AACCGCTTCT TGTCTCGTCA TGGTGCTCGC 
    TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA GGAATTAACC AGTGGCGAGA TCAACTTAAA 
    CCATCTCAGC TACTTCATCG TCTGTGTGAG AGGCGAAACT ACAAACTCCC CGTCTACAAG 
    CAGGATCGAA TTTACTTCAG AGGACATGAA TACACTGTCG CAGACCTTGA TGAAGGGAAA 
    CCTCTTAACC CTCACTTGGG ACCAGTGATG GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG 
    CATGGTTTGG TGCCTGAACA TGTCGAGACG CGACCCCTCT ACAGCCCCCT TCAGCCAGAT 
    ATAGAACAGG GGCGACTACA AATGTGGGTG GACCTGTTCC CTAAGTCTTT AGGCCCTCCA 
    GGTCCTCCAT TCAACATCAC CCCACGCAAA GCTAAAAAGT TCTTCCTGCG CTGTATTATA 
    TGGAACACAA GAGATGTAAT CTTGGATGAT GTCAGCGTTA CCGGGGAGAA GATGAGTGAC 
    ATTTATGTGA AAGGCTGGAT GCACGGCCAT GAGGAGAACA AGCAGAAAAC AGACGTGCAC 
    TACCGATCGC TGGGTGGAGA GGGTAACTTT AACTGGCGCT TCCTCTTCCC CTTCCACTAT 
    TTACCTGCGG AACAACTGTG CACCATTGAT AAAAAGGAGC ACTTTTGGAG TCTGGATAAA 
    GTTGAAACGA AGCTTCCTCC TAAACTTACT ATCCAGATTT GGGACAATGA CAAGTTCTCC 
    TTTGATGATT TCCTCGGCTC TTTGGAGATG GATCTGAACC ACATGCTGAG TCCTGCGAAG 
    TCTCCAGAGA AGTGTGGTCT TGAGATGCTC ACTCAGTCCC AGGACAAACT GGTCTCTTTG 
    CTTGAGCAGA AAACGGTGAA AGGCTGGTGG CCGTGTGTCT GTGACAAAGA AGACAAGAAG 
    ATACTTGCTG GAAAGGTGGA GATGTCTCTG GAAATAGTCT GTGAACAGGA GCAGGATGAG 
    AGGCCTGCAG GACTCGGCCG AGATGAGCCC AACATGAATC CCCATCTAGA GGAGCCTCAG 
    CGTCCCGAGA CATCATTCTT ATGGTTCTCG TCCCCATATA AGACGTTTAG GTTCATTGTG 
    TGGAGGAGGT TTAAGTGGCT TATTCTGGGC CTCTTTATAC TCCTTTTCAT TATTCTCTTC 
    CTTGGAGTAT TTCTCTACGC CTTCCCTAAC TATGCTGCTA TGAAGATGGT TGGACCCTTC 
    AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001244122.1
    CDS304..6570
    Translation

    Target ORF information:

    RefSeq Version NM_001257193.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001257193.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAGAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG GTAGCCTTTT TCCGACTTGT GGAAAAAATA 
    TTCAATAATT TTGAAAAAAG CTTATTAATT GTGGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CGAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GGTGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGGTAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG 
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT 
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG 
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG 
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG 
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC 
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT 
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT 
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT 
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC 
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG 
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC 
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG 
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA 
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA 
    AACAGTCCGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC 
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA 
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT 
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT 
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG 
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA 
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG 
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG 
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG 
    GGGTACACAG ATGTGAATGG AGAAAAGGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT 
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGC GAGGATCTGA AGCGTGCAGT GGATGATCAA 
    GGGTGGGAAT ATGGAATAAC CCTCCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT 
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG 
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA 
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT 
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CAGGGCCGGC GGCCATCTTT 
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT 
    ACAACGTTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT 
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC 
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTG CATCAGAGTC AGAAGACGGT GACTGTTCGA 
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA 
    GACCATCTTG TGACTGAGAG GAGTCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC 
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC 
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT 
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA 
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG 
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA 
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA 
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT 
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG 
    AACCCTAATT TTGACGTCAA CATCCTGTCC ATTGATGTCC GATTGCCTAA GGAAGAGCTC 
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG 
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG 
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG 
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGC AGGATGAATT TATGGACTGG 
    TGGAGCAAGT TTTATGCCTC CATGGGTGAA AGGACCAAGT GTGGGAGTTA TCTGGAGAAA 
    GGGTTCGATA TGCTGCAGGT GTATGACAGA GAACTGGAGA AGACTGAAGG CTTTGAGCAA 
    TTTGCTGATT TCTGTCAAAC GTTTAAACTT TACCGTGGAA GAACCCAGAA TGACAGCGAA 
    GATCCTTCCG TTGTTGGAGA GTTCAAGGGT TCGTTTAAGA TCTATCCATT GCCTGATGAT 
    CCCCCTATGC CTGTACCCAC ACGTCAGTTT CGCAAACTCC CCCCGAACGG TATAGAGGAG 
    TGCTTGGTAC GCATCTACAT CATCCAGGCT TTTGGGTTAC AGCCCAAAGA TGCCAATGGC 
    AAGTGTGACC CCTACGTTAA GATCACGCTT GGCAAGAAAT CAGTCGATGA CCACGACAAT 
    TACATACCCT GTACTTTGGA GCCCGTCTTT GGAAAGATGT TTGAGCTGAC CTGCTGTCTG 
    CCTCTTGAGA AAGACCTGAA GATTACACTC TATGATTACG ACATGGTGAC TAAAGATGAG 
    AAAATCGGAG AGACCATCAT TGATTTGGAG AACCGCTTCT TGTCTCGTCA TGGTGCTCGC 
    TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA GGAATTAACC AGTGGCGAGA TCAACTTAAA 
    CCATCTCAGC TACTTCATCG TCTGTGTGAG AGGCGAAACT ACAAACTCCC CGTCTACAAG 
    CAGGATCGAA TTTACTTCAG AGGACATGAA TACACTGTCG CAGACCTTGA TGAAGGGAAA 
    CCTCTTAACC CTCACTTGGG ACCAGTGATG GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG 
    CATGGTTTGG TGCCTGAACA TGTCGAGACG CGACCCCTCT ACAGCCCCCT TCAGCCAGAT 
    ATAGAACAGG GGCGACTACA AATGTGGGTG GACCTGTTCC CTAAGTCTTT AGGCCCTCCA 
    GGTCCTCCAT TCAACATCAC CCCACGCAAA GCTAAAAAGT TCTTCCTGCG CTGTATTATA 
    TGGAACACAA GAGATGTAAT CTTGGATGAT GTCAGCGTTA CCGGGGAGAA GATGAGTGAC 
    ATTTATGTGA AAGGCTGGAT GCACGGCCAT GAGGAGAACA AGCAGAAAAC AGACGTGCAC 
    TACCGATCGC TGGGTGGAGA GGGTAACTTT AACTGGCGCT TCCTCTTCCC CTTCCACTAT 
    TTACCTGCGG AACAACTGTG CACCATTGAT AAAAAGGAGC ACTTTTGGAG TCTGGATAAA 
    GTTGAAACGA AGCTTCCTCC TAAACTTACT ATCCAGATTT GGGACAATGA CAAGTTCTCC 
    TTTGATGATT TCCTCGGCTC TTTGGAGATG GATCTGAACC ACATGCTGAG TCCTGCGAAG 
    TCTCCAGAGA AGTGTGGTCT TGAGATGCTC ACTCAGTCCC AGGACAAACT GGTCTCTTTG 
    CTTGAGCAGA AAACGGTGAA AGGCTGGTGG CCGTGTGTCT GTGACAAAGA AGACAAGAAG 
    ATACTTGCTG GAAAGGTGGA GATGTCTCTG GAAATAGTCT GTGAACAGGA GCAGGATGAG 
    AGGCCTGCAG GACTCGGCCG AGATGAGCCC AACATGAATC CCCATCTAGA GGAGCCTCAG 
    CGTCCCGAGA CATCATTCTT ATGGTTCTCG TCCCCATATA AGACGTTTAG GTTCATTGTG 
    TGGAGGAGGT TTAAGTGGCT TATTCTGGGC CTCTTTATAC TCCTTTTCAT TATTCTCTTC 
    CTTGGAGTAT TTCTCTACGC CTTCCCTAAC TATGCTGCTA TGAAGATGGT TGGACCCTTC 
    AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    dysf ( XM_005172291.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172348.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa12912
    Clone ID Related Accession (Same CDS sequence) XM_005172291.3 , XM_005172291.4
    Accession Version XM_005172291.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product dysferlin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172291.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT 
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT 
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT 
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT 
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG 
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG 
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC 
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA 
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC 
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA 
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC 
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG 
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT 
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT 
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT 
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT 
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG 
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG 
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT 
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG 
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA 
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT 
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC 
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT 
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA 
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC 
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG 
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC 
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC 
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC 
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA 
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG 
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT 
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA 
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC 
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT 
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA 
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA 
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT 
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT 
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA 
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT 
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG 
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC 
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT 
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT 
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA 
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT 
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG 
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC 
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC 
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA 
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC 
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA 
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT 
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC 
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC 
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT 
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC 
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC 
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT 
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA 
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA 
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG 
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG 
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT 
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC 
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG 
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT 
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC 
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG 
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT 
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT 
    GGACAGGCAG ATGGCACCAG CTCTGCCATT ATTAACATCT CGCCTTCTCA GTCCAGCCTT 
    CATCAGGATG AATTTATGGA CTGGTGGAGC AAGTTTTATG CCTCCATGGG TGAGAGGACC 
    AAGTGTGGGA GTTATCTGGA GAAAGGGTTC GATATGCTGC AGGTGTATGA CAGAGAACTG 
    GAGAAGACTG AAGGCTTTGA GCAATTTGCT GATTTCTGTC AAACGTTTAA ACTTTACCGT 
    GGAAGAACCC AGAATGACAG CGAAGATCCT TCCGTTGTTG GAGAGTTCAA GGGTTCGTTT 
    AAGATCTATC CATTGCCTGA TGATCCCTCT ATGCCTGTAC CCACACGTCA GTTTCGCAAA 
    CTCCCCCCGA ACGGTATAGA GGAGTGCTTG GTACGCATCT ACATCATCCA GGCTTTTGGG 
    TTACAGCCCA AAGATGCCAA TGGCAAGTGT GACCCCTATG TTAAGATCAC GCTTGGCAAG 
    AAATCAGTCG ATGACCACGA CAATTACATA CCCTGTACTT TGGAGCCCGT CTTTGGAAAG 
    ATGTTTGAGC TGACCTGCTG TCTGCCTCTT GAGAAAGACC TGAAGATTAC ACTCTATGAT 
    TACGACATGG TGACTAAAGA TGAGAAAATC GGAGAGACCA TCATTGATTT GGAGAACCGC 
    TTCTTGTCTC GTCATGGTGC TCGCTGCGGC CTTCCGCAGT CCTACTGTCT GTCAGGAATT 
    AACCAGTGGC GAGATCAACT TAAACCATCT CAGCTACTTC ATCGTCTGTG TGAGAGGCGA 
    AACTACAAAC TCCCCGTCTA CAAGCAGGAT CGAATTTACT TCAGAGGACA TGAATACACT 
    GTCGCAGACC TTGATGAAGG GAAACCTCTT AACCCTCACT TGGGACCAGT GATGGAGCGT 
    CTGGCTCTGT TGGTGTTGAG GAGGCATGGT TTGGTGCCTG AACATGTCGA GACGCGACCC 
    CTCTACAGCC CCCTTCAGCC AGATATAGAA CAGGGGCGAC TACAAATGTG GGTGGACCTG 
    TTCCCTAAGT CTTTAGGCCC TCCAGGTCCT CCATTCAACA TCACCCCACG CAAAGCTAAA 
    AAGTTCTTCC TGCGCTGTAT TATATGGAAC ACAAGAGATG TAATCTTGGA TGATGTCAGC 
    GTTACCGGGG AGAAGATGAG TGACATTTAT GTGAAAGGCT GGATGCACGG CCATGAGGAG 
    AACAAGCAGA AAACAGACGT GCACTACCGA TCGCTGGGTG GTGAGGGTAA CTTTAACTGG 
    CGCTTCCTCT TCCCCTTCCA CTATTTACCT GCGGAACAAC TGTGCACCAT TGATAAAAAG 
    GAGCACTTTT GGAGTCTGGA TAAAGTTGAA ACGAAGCTTC CTCCTAAACT TACTATCCAG 
    ATTTGGGACA ATGACAAGTT CTCCTTTGAT GATTTCCTCG GCTCTTTGGA GATGGATCTG 
    AACCACATGC TGAGTCCTGC GAAGTCTCCA GAGAAGTGTG GTCTTGAGAT GCTCACTCAG 
    TCCCAGGACA AACTGGTCTC TTTGTTTGAG CAGAAAACGG TGAAAGGCTG GTGGCCGTGT 
    GTCTGTGACA AAGAAGACAA GAAGATACTT GCTGGAAAGG TGGAGATGTC TCTGGAAATA 
    GTCTGTGAAC AGGAGCAGGA TGAGAGGCCT GCAGGACTCG GCCGAGATGA GCCCAACATG 
    AATCCCCATC TAGAGGAGCC TCAGCGTCCC GAGACATCAT TCTTATGGTT CTCGTCCCCA 
    TATAAGACGT TTAGGTTCAT TGTGTGGAGG AGGTTTAAGT GGCTTATTCT GGGCCTCTTT 
    ATACTCTTTT TCATTATTCT CTTCCTTGGA GTATTTCTCT ACGCCTTCCC TAACTATGCT 
    GCTATGAAGA TGGTTGGACC CTTCAGTGGA CTAGGAAAGG GAACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172348.1
    CDS283..6633
    Translation

    Target ORF information:

    RefSeq Version XM_005172291.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005172291.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT 
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT 
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT 
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT 
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG 
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG 
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC 
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA 
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC 
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA 
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC 
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG 
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT 
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT 
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT 
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT 
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG 
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG 
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT 
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG 
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA 
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT 
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC 
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT 
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA 
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC 
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG 
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC 
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC 
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC 
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA 
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG 
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT 
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA 
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC 
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT 
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA 
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA 
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT 
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT 
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA 
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT 
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG 
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC 
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT 
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT 
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA 
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT 
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG 
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC 
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC 
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA 
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC 
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA 
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT 
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC 
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC 
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT 
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC 
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC 
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT 
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA 
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA 
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG 
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG 
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT 
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC 
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG 
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT 
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC 
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG 
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT 
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT 
    GGACAGGCAG ATGGCACCAG CTCTGCCATT ATTAACATCT CGCCTTCTCA GTCCAGCCTT 
    CATCAGGATG AATTTATGGA CTGGTGGAGC AAGTTTTATG CCTCCATGGG TGAGAGGACC 
    AAGTGTGGGA GTTATCTGGA GAAAGGGTTC GATATGCTGC AGGTGTATGA CAGAGAACTG 
    GAGAAGACTG AAGGCTTTGA GCAATTTGCT GATTTCTGTC AAACGTTTAA ACTTTACCGT 
    GGAAGAACCC AGAATGACAG CGAAGATCCT TCCGTTGTTG GAGAGTTCAA GGGTTCGTTT 
    AAGATCTATC CATTGCCTGA TGATCCCTCT ATGCCTGTAC CCACACGTCA GTTTCGCAAA 
    CTCCCCCCGA ACGGTATAGA GGAGTGCTTG GTACGCATCT ACATCATCCA GGCTTTTGGG 
    TTACAGCCCA AAGATGCCAA TGGCAAGTGT GACCCCTATG TTAAGATCAC GCTTGGCAAG 
    AAATCAGTCG ATGACCACGA CAATTACATA CCCTGTACTT TGGAGCCCGT CTTTGGAAAG 
    ATGTTTGAGC TGACCTGCTG TCTGCCTCTT GAGAAAGACC TGAAGATTAC ACTCTATGAT 
    TACGACATGG TGACTAAAGA TGAGAAAATC GGAGAGACCA TCATTGATTT GGAGAACCGC 
    TTCTTGTCTC GTCATGGTGC TCGCTGCGGC CTTCCGCAGT CCTACTGTCT GTCAGGAATT 
    AACCAGTGGC GAGATCAACT TAAACCATCT CAGCTACTTC ATCGTCTGTG TGAGAGGCGA 
    AACTACAAAC TCCCCGTCTA CAAGCAGGAT CGAATTTACT TCAGAGGACA TGAATACACT 
    GTCGCAGACC TTGATGAAGG GAAACCTCTT AACCCTCACT TGGGACCAGT GATGGAGCGT 
    CTGGCTCTGT TGGTGTTGAG GAGGCATGGT TTGGTGCCTG AACATGTCGA GACGCGACCC 
    CTCTACAGCC CCCTTCAGCC AGATATAGAA CAGGGGCGAC TACAAATGTG GGTGGACCTG 
    TTCCCTAAGT CTTTAGGCCC TCCAGGTCCT CCATTCAACA TCACCCCACG CAAAGCTAAA 
    AAGTTCTTCC TGCGCTGTAT TATATGGAAC ACAAGAGATG TAATCTTGGA TGATGTCAGC 
    GTTACCGGGG AGAAGATGAG TGACATTTAT GTGAAAGGCT GGATGCACGG CCATGAGGAG 
    AACAAGCAGA AAACAGACGT GCACTACCGA TCGCTGGGTG GTGAGGGTAA CTTTAACTGG 
    CGCTTCCTCT TCCCCTTCCA CTATTTACCT GCGGAACAAC TGTGCACCAT TGATAAAAAG 
    GAGCACTTTT GGAGTCTGGA TAAAGTTGAA ACGAAGCTTC CTCCTAAACT TACTATCCAG 
    ATTTGGGACA ATGACAAGTT CTCCTTTGAT GATTTCCTCG GCTCTTTGGA GATGGATCTG 
    AACCACATGC TGAGTCCTGC GAAGTCTCCA GAGAAGTGTG GTCTTGAGAT GCTCACTCAG 
    TCCCAGGACA AACTGGTCTC TTTGTTTGAG CAGAAAACGG TGAAAGGCTG GTGGCCGTGT 
    GTCTGTGACA AAGAAGACAA GAAGATACTT GCTGGAAAGG TGGAGATGTC TCTGGAAATA 
    GTCTGTGAAC AGGAGCAGGA TGAGAGGCCT GCAGGACTCG GCCGAGATGA GCCCAACATG 
    AATCCCCATC TAGAGGAGCC TCAGCGTCCC GAGACATCAT TCTTATGGTT CTCGTCCCCA 
    TATAAGACGT TTAGGTTCAT TGTGTGGAGG AGGTTTAAGT GGCTTATTCT GGGCCTCTTT 
    ATACTCTTTT TCATTATTCT CTTCCTTGGA GTATTTCTCT ACGCCTTCCC TAACTATGCT 
    GCTATGAAGA TGGTTGGACC CTTCAGTGGA CTAGGAAAGG GAACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    dysf ( XM_005172291.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172348.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa12912
    Clone ID Related Accession (Same CDS sequence) XM_005172291.3 , XM_005172291.4
    Accession Version XM_005172291.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172291.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT 
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT 
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT 
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT 
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG 
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG 
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC 
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA 
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC 
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA 
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC 
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG 
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT 
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT 
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT 
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT 
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG 
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG 
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT 
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG 
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA 
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT 
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC 
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT 
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA 
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC 
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG 
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC 
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC 
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC 
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA 
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG 
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT 
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA 
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC 
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT 
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA 
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA 
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT 
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT 
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA 
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT 
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG 
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC 
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT 
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT 
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA 
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT 
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG 
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC 
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC 
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA 
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC 
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA 
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT 
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC 
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC 
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT 
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC 
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC 
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT 
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA 
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA 
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG 
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG 
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT 
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC 
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG 
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT 
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC 
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG 
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT 
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT 
    GGACAGGCAG ATGGCACCAG CTCTGCCATT ATTAACATCT CGCCTTCTCA GTCCAGCCTT 
    CATCAGGATG AATTTATGGA CTGGTGGAGC AAGTTTTATG CCTCCATGGG TGAGAGGACC 
    AAGTGTGGGA GTTATCTGGA GAAAGGGTTC GATATGCTGC AGGTGTATGA CAGAGAACTG 
    GAGAAGACTG AAGGCTTTGA GCAATTTGCT GATTTCTGTC AAACGTTTAA ACTTTACCGT 
    GGAAGAACCC AGAATGACAG CGAAGATCCT TCCGTTGTTG GAGAGTTCAA GGGTTCGTTT 
    AAGATCTATC CATTGCCTGA TGATCCCTCT ATGCCTGTAC CCACACGTCA GTTTCGCAAA 
    CTCCCCCCGA ACGGTATAGA GGAGTGCTTG GTACGCATCT ACATCATCCA GGCTTTTGGG 
    TTACAGCCCA AAGATGCCAA TGGCAAGTGT GACCCCTATG TTAAGATCAC GCTTGGCAAG 
    AAATCAGTCG ATGACCACGA CAATTACATA CCCTGTACTT TGGAGCCCGT CTTTGGAAAG 
    ATGTTTGAGC TGACCTGCTG TCTGCCTCTT GAGAAAGACC TGAAGATTAC ACTCTATGAT 
    TACGACATGG TGACTAAAGA TGAGAAAATC GGAGAGACCA TCATTGATTT GGAGAACCGC 
    TTCTTGTCTC GTCATGGTGC TCGCTGCGGC CTTCCGCAGT CCTACTGTCT GTCAGGAATT 
    AACCAGTGGC GAGATCAACT TAAACCATCT CAGCTACTTC ATCGTCTGTG TGAGAGGCGA 
    AACTACAAAC TCCCCGTCTA CAAGCAGGAT CGAATTTACT TCAGAGGACA TGAATACACT 
    GTCGCAGACC TTGATGAAGG GAAACCTCTT AACCCTCACT TGGGACCAGT GATGGAGCGT 
    CTGGCTCTGT TGGTGTTGAG GAGGCATGGT TTGGTGCCTG AACATGTCGA GACGCGACCC 
    CTCTACAGCC CCCTTCAGCC AGATATAGAA CAGGGGCGAC TACAAATGTG GGTGGACCTG 
    TTCCCTAAGT CTTTAGGCCC TCCAGGTCCT CCATTCAACA TCACCCCACG CAAAGCTAAA 
    AAGTTCTTCC TGCGCTGTAT TATATGGAAC ACAAGAGATG TAATCTTGGA TGATGTCAGC 
    GTTACCGGGG AGAAGATGAG TGACATTTAT GTGAAAGGCT GGATGCACGG CCATGAGGAG 
    AACAAGCAGA AAACAGACGT GCACTACCGA TCGCTGGGTG GTGAGGGTAA CTTTAACTGG 
    CGCTTCCTCT TCCCCTTCCA CTATTTACCT GCGGAACAAC TGTGCACCAT TGATAAAAAG 
    GAGCACTTTT GGAGTCTGGA TAAAGTTGAA ACGAAGCTTC CTCCTAAACT TACTATCCAG 
    ATTTGGGACA ATGACAAGTT CTCCTTTGAT GATTTCCTCG GCTCTTTGGA GATGGATCTG 
    AACCACATGC TGAGTCCTGC GAAGTCTCCA GAGAAGTGTG GTCTTGAGAT GCTCACTCAG 
    TCCCAGGACA AACTGGTCTC TTTGTTTGAG CAGAAAACGG TGAAAGGCTG GTGGCCGTGT 
    GTCTGTGACA AAGAAGACAA GAAGATACTT GCTGGAAAGG TGGAGATGTC TCTGGAAATA 
    GTCTGTGAAC AGGAGCAGGA TGAGAGGCCT GCAGGACTCG GCCGAGATGA GCCCAACATG 
    AATCCCCATC TAGAGGAGCC TCAGCGTCCC GAGACATCAT TCTTATGGTT CTCGTCCCCA 
    TATAAGACGT TTAGGTTCAT TGTGTGGAGG AGGTTTAAGT GGCTTATTCT GGGCCTCTTT 
    ATACTCTTTT TCATTATTCT CTTCCTTGGA GTATTTCTCT ACGCCTTCCC TAACTATGCT 
    GCTATGAAGA TGGTTGGACC CTTCAGTGGA CTAGGAAAGG GAACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172348.1
    CDS358..6708
    Translation

    Target ORF information:

    RefSeq Version XM_005172291.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005172291.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT 
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT 
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT 
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT 
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG 
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG 
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC 
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA 
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC 
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA 
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC 
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG 
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT 
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT 
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT 
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT 
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG 
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG 
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT 
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG 
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA 
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT 
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC 
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT 
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA 
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC 
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG 
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC 
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC 
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC 
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA 
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG 
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT 
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA 
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC 
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT 
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA 
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA 
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT 
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT 
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA 
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT 
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG 
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC 
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT 
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT 
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA 
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT 
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG 
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC 
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC 
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA 
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC 
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA 
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT 
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC 
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC 
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT 
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC 
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC 
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT 
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA 
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA 
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG 
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG 
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT 
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC 
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG 
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT 
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC 
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG 
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT 
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT 
    GGACAGGCAG ATGGCACCAG CTCTGCCATT ATTAACATCT CGCCTTCTCA GTCCAGCCTT 
    CATCAGGATG AATTTATGGA CTGGTGGAGC AAGTTTTATG CCTCCATGGG TGAGAGGACC 
    AAGTGTGGGA GTTATCTGGA GAAAGGGTTC GATATGCTGC AGGTGTATGA CAGAGAACTG 
    GAGAAGACTG AAGGCTTTGA GCAATTTGCT GATTTCTGTC AAACGTTTAA ACTTTACCGT 
    GGAAGAACCC AGAATGACAG CGAAGATCCT TCCGTTGTTG GAGAGTTCAA GGGTTCGTTT 
    AAGATCTATC CATTGCCTGA TGATCCCTCT ATGCCTGTAC CCACACGTCA GTTTCGCAAA 
    CTCCCCCCGA ACGGTATAGA GGAGTGCTTG GTACGCATCT ACATCATCCA GGCTTTTGGG 
    TTACAGCCCA AAGATGCCAA TGGCAAGTGT GACCCCTATG TTAAGATCAC GCTTGGCAAG 
    AAATCAGTCG ATGACCACGA CAATTACATA CCCTGTACTT TGGAGCCCGT CTTTGGAAAG 
    ATGTTTGAGC TGACCTGCTG TCTGCCTCTT GAGAAAGACC TGAAGATTAC ACTCTATGAT 
    TACGACATGG TGACTAAAGA TGAGAAAATC GGAGAGACCA TCATTGATTT GGAGAACCGC 
    TTCTTGTCTC GTCATGGTGC TCGCTGCGGC CTTCCGCAGT CCTACTGTCT GTCAGGAATT 
    AACCAGTGGC GAGATCAACT TAAACCATCT CAGCTACTTC ATCGTCTGTG TGAGAGGCGA 
    AACTACAAAC TCCCCGTCTA CAAGCAGGAT CGAATTTACT TCAGAGGACA TGAATACACT 
    GTCGCAGACC TTGATGAAGG GAAACCTCTT AACCCTCACT TGGGACCAGT GATGGAGCGT 
    CTGGCTCTGT TGGTGTTGAG GAGGCATGGT TTGGTGCCTG AACATGTCGA GACGCGACCC 
    CTCTACAGCC CCCTTCAGCC AGATATAGAA CAGGGGCGAC TACAAATGTG GGTGGACCTG 
    TTCCCTAAGT CTTTAGGCCC TCCAGGTCCT CCATTCAACA TCACCCCACG CAAAGCTAAA 
    AAGTTCTTCC TGCGCTGTAT TATATGGAAC ACAAGAGATG TAATCTTGGA TGATGTCAGC 
    GTTACCGGGG AGAAGATGAG TGACATTTAT GTGAAAGGCT GGATGCACGG CCATGAGGAG 
    AACAAGCAGA AAACAGACGT GCACTACCGA TCGCTGGGTG GTGAGGGTAA CTTTAACTGG 
    CGCTTCCTCT TCCCCTTCCA CTATTTACCT GCGGAACAAC TGTGCACCAT TGATAAAAAG 
    GAGCACTTTT GGAGTCTGGA TAAAGTTGAA ACGAAGCTTC CTCCTAAACT TACTATCCAG 
    ATTTGGGACA ATGACAAGTT CTCCTTTGAT GATTTCCTCG GCTCTTTGGA GATGGATCTG 
    AACCACATGC TGAGTCCTGC GAAGTCTCCA GAGAAGTGTG GTCTTGAGAT GCTCACTCAG 
    TCCCAGGACA AACTGGTCTC TTTGTTTGAG CAGAAAACGG TGAAAGGCTG GTGGCCGTGT 
    GTCTGTGACA AAGAAGACAA GAAGATACTT GCTGGAAAGG TGGAGATGTC TCTGGAAATA 
    GTCTGTGAAC AGGAGCAGGA TGAGAGGCCT GCAGGACTCG GCCGAGATGA GCCCAACATG 
    AATCCCCATC TAGAGGAGCC TCAGCGTCCC GAGACATCAT TCTTATGGTT CTCGTCCCCA 
    TATAAGACGT TTAGGTTCAT TGTGTGGAGG AGGTTTAAGT GGCTTATTCT GGGCCTCTTT 
    ATACTCTTTT TCATTATTCT CTTCCTTGGA GTATTTCTCT ACGCCTTCCC TAACTATGCT 
    GCTATGAAGA TGGTTGGACC CTTCAGTGGA CTAGGAAAGG GAACACAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    dysf ( XM_005172293.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172350.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa12914
    Clone ID Related Accession (Same CDS sequence) XM_005172293.3 , XM_005172293.4
    Accession Version XM_005172293.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product dysferlin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172293.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT 
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT 
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT 
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT 
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG 
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG 
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC 
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA 
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC 
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA 
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC 
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG 
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT 
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT 
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT 
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT 
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG 
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG 
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT 
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG 
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA 
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT 
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC 
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT 
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA 
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC 
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG 
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC 
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC 
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC 
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA 
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG 
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT 
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA 
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC 
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT 
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA 
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA 
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT 
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT 
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA 
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT 
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG 
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC 
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT 
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT 
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA 
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT 
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG 
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC 
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC 
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA 
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC 
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA 
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT 
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC 
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC 
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT 
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC 
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC 
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT 
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA 
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA 
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG 
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG 
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT 
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC 
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG 
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT 
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC 
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG 
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT 
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT 
    GGACAGCAGG ATGAATTTAT GGACTGGTGG AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG 
    ACCAAGTGTG GGAGTTATCT GGAGAAAGGG TTCGATATGC TGCAGGTGTA TGACAGAGAA 
    CTGGAGAAGA CTGAAGGCTT TGAGCAATTT GCTGATTTCT GTCAAACGTT TAAACTTTAC 
    CGTGGAAGAA CCCAGAATGA CAGCGAAGAT CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG 
    TTTAAGATCT ATCCATTGCC TGATGATCCC TCTATGCCTG TACCCACACG TCAGTTTCGC 
    AAACTCCCCC CGAACGGTAT AGAGGAGTGC TTGGTACGCA TCTACATCAT CCAGGCTTTT 
    GGGTTACAGC CCAAAGATGC CAATGGCAAG TGTGACCCCT ATGTTAAGAT CACGCTTGGC 
    AAGAAATCAG TCGATGACCA CGACAATTAC ATACCCTGTA CTTTGGAGCC CGTCTTTGGA 
    AAGATGTTTG AGCTGACCTG CTGTCTGCCT CTTGAGAAAG ACCTGAAGAT TACACTCTAT 
    GATTACGACA TGGTGACTAA AGATGAGAAA ATCGGAGAGA CCATCATTGA TTTGGAGAAC 
    CGCTTCTTGT CTCGTCATGG TGCTCGCTGC GGCCTTCCGC AGTCCTACTG TCTGTCAGGA 
    ATTAACCAGT GGCGAGATCA ACTTAAACCA TCTCAGCTAC TTCATCGTCT GTGTGAGAGG 
    CGAAACTACA AACTCCCCGT CTACAAGCAG GATCGAATTT ACTTCAGAGG ACATGAATAC 
    ACTGTCGCAG ACCTTGATGA AGGGAAACCT CTTAACCCTC ACTTGGGACC AGTGATGGAG 
    CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT GGTTTGGTGC CTGAACATGT CGAGACGCGA 
    CCCCTCTACA GCCCCCTTCA GCCAGATATA GAACAGGGGC GACTACAAAT GTGGGTGGAC 
    CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT CCTCCATTCA ACATCACCCC ACGCAAAGCT 
    AAAAAGTTCT TCCTGCGCTG TATTATATGG AACACAAGAG ATGTAATCTT GGATGATGTC 
    AGCGTTACCG GGGAGAAGAT GAGTGACATT TATGTGAAAG GCTGGATGCA CGGCCATGAG 
    GAGAACAAGC AGAAAACAGA CGTGCACTAC CGATCGCTGG GTGGTGAGGG TAACTTTAAC 
    TGGCGCTTCC TCTTCCCCTT CCACTATTTA CCTGCGGAAC AACTGTGCAC CATTGATAAA 
    AAGGAGCACT TTTGGAGTCT GGATAAAGTT GAAACGAAGC TTCCTCCTAA ACTTACTATC 
    CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGATTTCC TCGGCTCTTT GGAGATGGAT 
    CTGAACCACA TGCTGAGTCC TGCGAAGTCT CCAGAGAAGT GTGGTCTTGA GATGCTCACT 
    CAGTCCCAGG ACAAACTGGT CTCTTTGTTT GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG 
    TGTGTCTGTG ACAAAGAAGA CAAGAAGATA CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA 
    ATAGTCTGTG AACAGGAGCA GGATGAGAGG CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC 
    ATGAATCCCC ATCTAGAGGA GCCTCAGCGT CCCGAGACAT CATTCTTATG GTTCTCGTCC 
    CCATATAAGA CGTTTAGGTT CATTGTGTGG AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC 
    TTTATACTCT TTTTCATTAT TCTCTTCCTT GGAGTATTTC TCTACGCCTT CCCTAACTAT 
    GCTGCTATGA AGATGGTTGG ACCCTTCAGT GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172350.1
    CDS283..6576
    Translation

    Target ORF information:

    RefSeq Version XM_005172293.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005172293.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT 
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT 
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT 
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT 
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG 
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG 
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC 
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA 
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC 
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA 
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC 
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG 
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT 
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT 
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT 
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT 
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG 
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG 
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT 
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG 
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA 
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT 
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC 
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT 
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA 
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC 
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG 
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC 
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC 
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC 
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA 
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG 
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT 
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA 
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC 
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT 
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA 
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA 
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT 
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT 
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA 
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT 
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG 
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC 
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT 
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT 
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA 
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT 
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG 
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC 
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC 
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA 
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC 
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA 
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT 
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC 
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC 
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT 
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC 
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC 
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT 
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA 
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA 
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG 
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG 
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT 
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC 
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG 
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT 
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC 
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG 
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT 
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT 
    GGACAGCAGG ATGAATTTAT GGACTGGTGG AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG 
    ACCAAGTGTG GGAGTTATCT GGAGAAAGGG TTCGATATGC TGCAGGTGTA TGACAGAGAA 
    CTGGAGAAGA CTGAAGGCTT TGAGCAATTT GCTGATTTCT GTCAAACGTT TAAACTTTAC 
    CGTGGAAGAA CCCAGAATGA CAGCGAAGAT CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG 
    TTTAAGATCT ATCCATTGCC TGATGATCCC TCTATGCCTG TACCCACACG TCAGTTTCGC 
    AAACTCCCCC CGAACGGTAT AGAGGAGTGC TTGGTACGCA TCTACATCAT CCAGGCTTTT 
    GGGTTACAGC CCAAAGATGC CAATGGCAAG TGTGACCCCT ATGTTAAGAT CACGCTTGGC 
    AAGAAATCAG TCGATGACCA CGACAATTAC ATACCCTGTA CTTTGGAGCC CGTCTTTGGA 
    AAGATGTTTG AGCTGACCTG CTGTCTGCCT CTTGAGAAAG ACCTGAAGAT TACACTCTAT 
    GATTACGACA TGGTGACTAA AGATGAGAAA ATCGGAGAGA CCATCATTGA TTTGGAGAAC 
    CGCTTCTTGT CTCGTCATGG TGCTCGCTGC GGCCTTCCGC AGTCCTACTG TCTGTCAGGA 
    ATTAACCAGT GGCGAGATCA ACTTAAACCA TCTCAGCTAC TTCATCGTCT GTGTGAGAGG 
    CGAAACTACA AACTCCCCGT CTACAAGCAG GATCGAATTT ACTTCAGAGG ACATGAATAC 
    ACTGTCGCAG ACCTTGATGA AGGGAAACCT CTTAACCCTC ACTTGGGACC AGTGATGGAG 
    CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT GGTTTGGTGC CTGAACATGT CGAGACGCGA 
    CCCCTCTACA GCCCCCTTCA GCCAGATATA GAACAGGGGC GACTACAAAT GTGGGTGGAC 
    CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT CCTCCATTCA ACATCACCCC ACGCAAAGCT 
    AAAAAGTTCT TCCTGCGCTG TATTATATGG AACACAAGAG ATGTAATCTT GGATGATGTC 
    AGCGTTACCG GGGAGAAGAT GAGTGACATT TATGTGAAAG GCTGGATGCA CGGCCATGAG 
    GAGAACAAGC AGAAAACAGA CGTGCACTAC CGATCGCTGG GTGGTGAGGG TAACTTTAAC 
    TGGCGCTTCC TCTTCCCCTT CCACTATTTA CCTGCGGAAC AACTGTGCAC CATTGATAAA 
    AAGGAGCACT TTTGGAGTCT GGATAAAGTT GAAACGAAGC TTCCTCCTAA ACTTACTATC 
    CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGATTTCC TCGGCTCTTT GGAGATGGAT 
    CTGAACCACA TGCTGAGTCC TGCGAAGTCT CCAGAGAAGT GTGGTCTTGA GATGCTCACT 
    CAGTCCCAGG ACAAACTGGT CTCTTTGTTT GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG 
    TGTGTCTGTG ACAAAGAAGA CAAGAAGATA CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA 
    ATAGTCTGTG AACAGGAGCA GGATGAGAGG CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC 
    ATGAATCCCC ATCTAGAGGA GCCTCAGCGT CCCGAGACAT CATTCTTATG GTTCTCGTCC 
    CCATATAAGA CGTTTAGGTT CATTGTGTGG AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC 
    TTTATACTCT TTTTCATTAT TCTCTTCCTT GGAGTATTTC TCTACGCCTT CCCTAACTAT 
    GCTGCTATGA AGATGGTTGG ACCCTTCAGT GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    dysf ( XM_005172293.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172350.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID ODa12914
    Clone ID Related Accession (Same CDS sequence) XM_005172293.3 , XM_005172293.4
    Accession Version XM_005172293.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172293.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT 
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT 
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT 
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT 
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG 
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG 
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC 
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA 
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC 
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA 
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC 
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG 
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT 
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT 
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT 
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT 
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG 
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG 
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT 
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG 
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA 
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT 
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC 
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT 
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA 
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC 
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG 
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC 
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC 
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC 
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA 
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG 
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT 
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA 
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC 
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT 
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA 
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA 
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT 
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT 
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA 
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT 
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG 
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC 
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT 
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT 
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA 
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT 
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG 
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC 
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC 
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA 
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC 
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA 
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT 
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC 
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC 
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT 
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC 
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC 
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT 
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA 
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA 
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG 
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG 
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT 
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC 
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG 
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT 
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC 
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG 
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT 
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT 
    GGACAGCAGG ATGAATTTAT GGACTGGTGG AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG 
    ACCAAGTGTG GGAGTTATCT GGAGAAAGGG TTCGATATGC TGCAGGTGTA TGACAGAGAA 
    CTGGAGAAGA CTGAAGGCTT TGAGCAATTT GCTGATTTCT GTCAAACGTT TAAACTTTAC 
    CGTGGAAGAA CCCAGAATGA CAGCGAAGAT CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG 
    TTTAAGATCT ATCCATTGCC TGATGATCCC TCTATGCCTG TACCCACACG TCAGTTTCGC 
    AAACTCCCCC CGAACGGTAT AGAGGAGTGC TTGGTACGCA TCTACATCAT CCAGGCTTTT 
    GGGTTACAGC CCAAAGATGC CAATGGCAAG TGTGACCCCT ATGTTAAGAT CACGCTTGGC 
    AAGAAATCAG TCGATGACCA CGACAATTAC ATACCCTGTA CTTTGGAGCC CGTCTTTGGA 
    AAGATGTTTG AGCTGACCTG CTGTCTGCCT CTTGAGAAAG ACCTGAAGAT TACACTCTAT 
    GATTACGACA TGGTGACTAA AGATGAGAAA ATCGGAGAGA CCATCATTGA TTTGGAGAAC 
    CGCTTCTTGT CTCGTCATGG TGCTCGCTGC GGCCTTCCGC AGTCCTACTG TCTGTCAGGA 
    ATTAACCAGT GGCGAGATCA ACTTAAACCA TCTCAGCTAC TTCATCGTCT GTGTGAGAGG 
    CGAAACTACA AACTCCCCGT CTACAAGCAG GATCGAATTT ACTTCAGAGG ACATGAATAC 
    ACTGTCGCAG ACCTTGATGA AGGGAAACCT CTTAACCCTC ACTTGGGACC AGTGATGGAG 
    CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT GGTTTGGTGC CTGAACATGT CGAGACGCGA 
    CCCCTCTACA GCCCCCTTCA GCCAGATATA GAACAGGGGC GACTACAAAT GTGGGTGGAC 
    CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT CCTCCATTCA ACATCACCCC ACGCAAAGCT 
    AAAAAGTTCT TCCTGCGCTG TATTATATGG AACACAAGAG ATGTAATCTT GGATGATGTC 
    AGCGTTACCG GGGAGAAGAT GAGTGACATT TATGTGAAAG GCTGGATGCA CGGCCATGAG 
    GAGAACAAGC AGAAAACAGA CGTGCACTAC CGATCGCTGG GTGGTGAGGG TAACTTTAAC 
    TGGCGCTTCC TCTTCCCCTT CCACTATTTA CCTGCGGAAC AACTGTGCAC CATTGATAAA 
    AAGGAGCACT TTTGGAGTCT GGATAAAGTT GAAACGAAGC TTCCTCCTAA ACTTACTATC 
    CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGATTTCC TCGGCTCTTT GGAGATGGAT 
    CTGAACCACA TGCTGAGTCC TGCGAAGTCT CCAGAGAAGT GTGGTCTTGA GATGCTCACT 
    CAGTCCCAGG ACAAACTGGT CTCTTTGTTT GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG 
    TGTGTCTGTG ACAAAGAAGA CAAGAAGATA CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA 
    ATAGTCTGTG AACAGGAGCA GGATGAGAGG CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC 
    ATGAATCCCC ATCTAGAGGA GCCTCAGCGT CCCGAGACAT CATTCTTATG GTTCTCGTCC 
    CCATATAAGA CGTTTAGGTT CATTGTGTGG AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC 
    TTTATACTCT TTTTCATTAT TCTCTTCCTT GGAGTATTTC TCTACGCCTT CCCTAACTAT 
    GCTGCTATGA AGATGGTTGG ACCCTTCAGT GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172350.1
    CDS358..6651
    Translation

    Target ORF information:

    RefSeq Version XM_005172293.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005172293.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCTGCGTG TGTTTATTCT GTGCGCCCAG AATGTGCTGA CGCATGATGA GGACATTAGC 
    GATGCCTACT GCACCGTCAC TTATGAAGGG ACAAAAAAGA AGACTAAAGT CATCAAAAAT 
    AATGTCAATC CTGTCTGGAA TGAGGGCTTT GAGTGGGACC TGAAGGGTGT TCCTTTAGAT 
    TCGGGAGCAG AGCTTCATGT TGTCGTTAAA GACCATGAGA AAATGGGCAG GAATAGGTTT 
    CTGGGGGAGT GTCGAGTTGC TCTTCGAGAT GTGCTCAATT CTCCAAACCT GGCTGCTACT 
    TTTACCGTGT CCCTGGTCGA CACAAAGAGA AACAGCACTG GGGCCACAAT AACCCTGCAG 
    GTCTCCTACA TCCCTCCTCC AGGGATGACG CCCATCTTTC AGCCTCCTCC ACAGCCAGAG 
    GCCCAGCACA CACCGGTGGA GCTGGACACA GTCACAGTTT TCTCATTGGA CACTATGGGC 
    GAGGATGACA CTGAGAGCAT GCTGATGATG GAGGTGGCGG AGGAGCCTGA ACCTGTTCCA 
    GGACCCCAGG GGCAGGACAT CGGCGCCCCT ACAGCACCAC CCAAAAAGGC TCCACCCAAC 
    TTTAACCAGG GACTAAAGAA AAAGAAACGC CAAAGCAACA AATCCCCCCT GTCCGACAAA 
    GCCCAGGACC TCCAGGTTCG GCTGCGCATC ATTGAAGGAA GGCAGCTTCC TGGTGTCAAC 
    ATCAAACCAG TCGTCAAAGT CACAGTTGGA GGACAGACGA AGAGAACCCG CATTAAAAAG 
    GGCAATAATC CATTTTTTGA TGAGACCTTT TTTCTAAACT TCTTTGAGAA ACCTTCAGAT 
    CTTTTTGATG AGCCAATATT CATAACCGTA TTTGATTCCA AGTCACTACG GAAAGACTCT 
    GTCATTGGAG AATTCAAGCT GGATGTTGGC ATGGTTTATA ATGAACACCG ACATGCTATT 
    CTCAGAAAAT GGTTGTTGCT GGCAGACCCT GATGATCTTT CTGCAGGGGC CAGAGGTTAT 
    TTGAAGGTCA GCATGTTTGT GCTGGCCGCA GGAGATGAAC CTCCGATGGA TAAGAAAGAG 
    GGTGTGGAGG AAAAGGAGGA CATAGAGGCC AATCTGTTGA GACCAGCTGG TCTAACCCTG 
    AGAGGAGCCA CGTTCACTCT GAGAGTCTAC AGAGCAGAAG ACCTTCCTCA GATGGATGAT 
    GCTCTTATTG ACGATGTCAA ACATTTCCTT GGCTTTGACA CTAATCGAAA GAACCTGGTG 
    GATCCATTGG TGGAGGTCAA CTTTGCAGGA AAAACGATAA GTAGTAAGAT TCTGGAGAAA 
    TGCGCTAACC CTCAGTGGAA CCAGTGCCTC ACTCTGCCCA TCAGGTTTCC ATCCATGTGT 
    GAAAAGATGA GAATACGTAT TTTAGATTGG GATCGTGCGA GTCATAATGA TGTCATTGGC 
    ACTGCACACT TATGCATGTC TAAAATCTCA GCCCCTGGAG GAGATATTGA AGATGAGTTT 
    CCTGCCAGGA CCCTCCACGA CACTATGGAT GACAGTATAG GCTTTCTTCC GACCTTTGGA 
    CCCTGCTTTG TCAACATGTA TGGGAGTCCT CGAGAGTTCA CAGCTTTCTC AGACCCACAC 
    GAGGCACTAA ACCTGGGAAA GGGAGAGGGT GTGGCCTATC GGGGACGTGT GTTGTTGGAG 
    CTCATAACAA AGCTTTCTGA TCAGGTGGAC CAAAGGTCTG AAGACATCAA CTCTGATGAC 
    CTGCTAGTGG TAGAAAAATT CATGCGGAAG AGGAAGTTTT CCCTGTTTGC TGCGTTCTAC 
    TCGGCCACTC TCCTTCAAGA TGTGGACGAT GCAGTTCAGT TTGAAGTCAG TATCGGTAAC 
    TACGGCAACA AGTTCGACAA CACCTGTTTG CCTCTGGCAT CCACCACTCA ATTCAGCAGA 
    GCTGTGTTCG ACGGCTGCCA TTACTATTAC CTGCCATGGG GAAATGTGAA ACCTGTGGTG 
    GTGGTGTCTT CAGAGTGGGA AGACATTGGC CCTCGTATAG AGGCCCTCAA CATGCTGCTT 
    AGCATAGTCG ACCGACTGGA GTCAAACTTG GAACAGGTAC AGCTGGAGCT CAAAGCAAGA 
    AGTGACGATC AGGAAGTCGA GCAGAGGGTT GTCCAGCTGC TTGATGATGT CATTGCAGAC 
    TGCAGTGAGG AACTCCCTGA GTTGGACAAA TGGCCTTGTG TGACTCCACT GGATAAAGCT 
    CTGAGGAATC TGCGCAAGAT TAACATGCAG CAGATAGTGC AGGCGGCCCT GGCACTCAAA 
    CATGGACCAG AGACTGAACT GAGTGCAGTG CTGGAGCAGG CTGAGGACTG GACCATGAGA 
    CTACGCTACC TCTCTGAGGA GCCCCAAAAC AGTCTGCCAG ATATAATAAT CTGGATGCTT 
    CAGGGAGACA GACGAGTGGC CTACCACAGA ATCCCTGCCC ATCAAGTTCT CTTTTCCTAT 
    GGTTACTGTG GCAAGCACTG TGGCCAACTT CAGACGGTTT TCATGAAGTG TCCACAGGAA 
    GATGGGGGAT GTAGTAAAAT CCCAGCTCAG CTCAGAATCA AGGTGTGGTT CGGACTGACT 
    GCGGATGTCA AATCTTTTAA CCAGTTTGCA GAGGGCAAAC TCTCAGTGTT TGCAGAAACG 
    TATGAGAACC AGACTAAACT GGCTCTGGTG GGGAACTGGG GTACGACGGG TCTGACTTAC 
    CCCAAGTTCA GTGATGTGAC CGGCAGAGTC AAACTGCCAA AGGAGAGCTT CAAACCCTCT 
    TCAGGCTGGA GCTGGGCAGG AGAATGGTTC ATCAGCCCAG AGAAAACGAT GCTGTATGAT 
    GCTGATGCAG GTCATATAAG CTTTTTGGAG GAGGTGTTTG AAAATCAGTT GCGGTTGCCA 
    GGAGGACAGT GGATTGGCAT GCCGGAGGGG TACACAGATG TGAATGGAGA AAAAGCTGTT 
    CCTAAAGATG AGATCGAGTG TCCCCCTGGC TGGGTGTGGG AGGATATTGA GTGGAGTGAG 
    GATCTGAAGC GTGCAGTGGA TGATCAAGGG TGGGAATATG GAATAACCCT TCCCCCGGAC 
    CGCCGACCAA AGTCCTGGGT TCCATCTGAG AAGATGTACC ATACCAACCG TCGGAGACGC 
    TGGATCAGAC TCCGCCGTAG AGACATGCAG AAAATGGAGG CGCTCAGAAA ACAGCGGCCA 
    GACGAGTCCG AGCGTGAGGG CTGGGAATAT GCCTCTTTAT TTGGCTGGAA GTTTCACCTC 
    AAGCAGAAGA AAACCGACTT GTTCCGTCGC CGGCGCTGGA GACGTCGAAT GGAGCCATTA 
    GAAAAGACGG GGCCGGCGGC CATCTTTGCC TTGGAGTGTT CGCTGAGCAG CTTTACTGAT 
    GAAAAGAATG ACGATAAGTC AGTCACTACA ACATTTGGTG TCAGCCGGCC GACTATCTCC 
    TGCTTCTTTG ATAGTGGGAC CCTGTATCAT TTGCGCTGTT ACTTGTATCA GGCCAGAGAC 
    CTGCTGGCTA TGGACAAGGA CAGTTTCTCG GATGCCTATG CCGTTGTATC GTTCCTTCAT 
    CAGAGTCAGA AGACGGTGAC TGTTCGAAAC ACACTCAACC CCACCTGGGA CCAGACCCTC 
    ATTTTCTACG AAGTGGAAAT TTTTGGAGAC CATCTTGTGA CTGAGAGGAG CCCTCCAAAC 
    ATTGTGGTGG AGCTGTACGA TCAGGACACC TATGGAGCTG ATGAGTTCAT GGGCCGCTGT 
    GTTTGCCAAC CATCAATGGC ATCTTCCCCT CGCCTCGCCT GGTATCCCAT CCAGAAAGCA 
    GGGAATTCTG CAGGAGATCT GCTTGCTGCT TTTGAACTGA TCCGCAGAGA TAAACCTGCA 
    GTTCATCACA TCCCGGGTCA AGAGGGAGAT ATTATACCAC CCTCACATAT CATGGATGAG 
    CTTGTGCTTC CAGGGTTCCT CTCTGAGAAT CAACAGCAAT GGCCAGAGGA CTCTGATTTG 
    CCCTATCCAC CACCTCAGAG GGAGCCAAAC GTCTACATGG TGCCACAGGG GATAAAACCT 
    GTCCTACAGC GCACTGCTAT TGAGATACTG GCATGGGGTT TGCGTAACCT GAAGAGTTTC 
    CAGCTGGCCA GTGTCACGTC TCCCAGTCTT CAGGTGGAAT GTGGCGGATC TGTGGTTCAG 
    TCGTGCGTCA TTCGAAATAT TAAGAAGAAC CCTAATTTTG ACGTCAACAT CCTGTTCATT 
    GATGTCCGAT TGCCTAAGGA AGAGCTCTAC ATGCCCCCAT TGGTGATCAA GGTTATAGAC 
    AACCGGCAGT TTGGCAGGAA GCCTGTGGTG GGCCAGTGTT CAGTCCACTC GCTCAATGAG 
    TTCTACTTCC AGCCGGAAAG TCTGGAGAGA GACTCAACAG AAGAGGAATT TGAAGTTATT 
    GCTCACACAC CTAGAGATGA TGTTCTGGTT GATATTGATG ACAAGGAGCC TCTAATCCCT 
    GGACAGCAGG ATGAATTTAT GGACTGGTGG AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG 
    ACCAAGTGTG GGAGTTATCT GGAGAAAGGG TTCGATATGC TGCAGGTGTA TGACAGAGAA 
    CTGGAGAAGA CTGAAGGCTT TGAGCAATTT GCTGATTTCT GTCAAACGTT TAAACTTTAC 
    CGTGGAAGAA CCCAGAATGA CAGCGAAGAT CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG 
    TTTAAGATCT ATCCATTGCC TGATGATCCC TCTATGCCTG TACCCACACG TCAGTTTCGC 
    AAACTCCCCC CGAACGGTAT AGAGGAGTGC TTGGTACGCA TCTACATCAT CCAGGCTTTT 
    GGGTTACAGC CCAAAGATGC CAATGGCAAG TGTGACCCCT ATGTTAAGAT CACGCTTGGC 
    AAGAAATCAG TCGATGACCA CGACAATTAC ATACCCTGTA CTTTGGAGCC CGTCTTTGGA 
    AAGATGTTTG AGCTGACCTG CTGTCTGCCT CTTGAGAAAG ACCTGAAGAT TACACTCTAT 
    GATTACGACA TGGTGACTAA AGATGAGAAA ATCGGAGAGA CCATCATTGA TTTGGAGAAC 
    CGCTTCTTGT CTCGTCATGG TGCTCGCTGC GGCCTTCCGC AGTCCTACTG TCTGTCAGGA 
    ATTAACCAGT GGCGAGATCA ACTTAAACCA TCTCAGCTAC TTCATCGTCT GTGTGAGAGG 
    CGAAACTACA AACTCCCCGT CTACAAGCAG GATCGAATTT ACTTCAGAGG ACATGAATAC 
    ACTGTCGCAG ACCTTGATGA AGGGAAACCT CTTAACCCTC ACTTGGGACC AGTGATGGAG 
    CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT GGTTTGGTGC CTGAACATGT CGAGACGCGA 
    CCCCTCTACA GCCCCCTTCA GCCAGATATA GAACAGGGGC GACTACAAAT GTGGGTGGAC 
    CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT CCTCCATTCA ACATCACCCC ACGCAAAGCT 
    AAAAAGTTCT TCCTGCGCTG TATTATATGG AACACAAGAG ATGTAATCTT GGATGATGTC 
    AGCGTTACCG GGGAGAAGAT GAGTGACATT TATGTGAAAG GCTGGATGCA CGGCCATGAG 
    GAGAACAAGC AGAAAACAGA CGTGCACTAC CGATCGCTGG GTGGTGAGGG TAACTTTAAC 
    TGGCGCTTCC TCTTCCCCTT CCACTATTTA CCTGCGGAAC AACTGTGCAC CATTGATAAA 
    AAGGAGCACT TTTGGAGTCT GGATAAAGTT GAAACGAAGC TTCCTCCTAA ACTTACTATC 
    CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGATTTCC TCGGCTCTTT GGAGATGGAT 
    CTGAACCACA TGCTGAGTCC TGCGAAGTCT CCAGAGAAGT GTGGTCTTGA GATGCTCACT 
    CAGTCCCAGG ACAAACTGGT CTCTTTGTTT GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG 
    TGTGTCTGTG ACAAAGAAGA CAAGAAGATA CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA 
    ATAGTCTGTG AACAGGAGCA GGATGAGAGG CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC 
    ATGAATCCCC ATCTAGAGGA GCCTCAGCGT CCCGAGACAT CATTCTTATG GTTCTCGTCC 
    CCATATAAGA CGTTTAGGTT CATTGTGTGG AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC 
    TTTATACTCT TTTTCATTAT TCTCTTCCTT GGAGTATTTC TCTACGCCTT CCCTAACTAT 
    GCTGCTATGA AGATGGTTGG ACCCTTCAGT GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    dysf ( XM_021477555.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333230.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa143308
    Clone ID Related Accession (Same CDS sequence) XM_021477555.1
    Accession Version XM_021477555.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT 
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC 
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC 
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG 
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT 
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG 
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC 
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC 
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT 
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG 
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA 
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT 
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT 
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA 
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC 
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG 
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT 
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA 
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA 
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA 
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG 
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA 
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG 
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG 
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA 
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG 
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC 
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG 
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG 
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT 
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG 
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT 
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT 
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC 
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC 
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG 
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT 
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC 
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG 
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA 
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG 
    GATGAGCTTG TGCTTCCAGG GTTCCTCTCT GAGAATCAAC AGCAATGGCC AGAGGACTCT 
    GATTTGCCCT ATCCACCACC TCAGAGGGAG CCAAACGTCT ACATGGTGCC ACAGGGGATA 
    AAACCTGTCC TACAGCGCAC TGCTATTGAG ATACTGGCAT GGGGTTTGCG TAACCTGAAG 
    AGTTTCCAGC TGGCCAGTGT CACGTCTCCC AGTCTTCAGG TGGAATGTGG CGGATCTGTG 
    GTTCAGTCGT GCGTCATTCG AAATATTAAG AAGAACCCTA ATTTTGACGT CAACATCCTG 
    TTCATTGATG TCCGATTGCC TAAGGAAGAG CTCTACATGC CCCCATTGGT GATCAAGGTT 
    ATAGACAACC GGCAGTTTGG CAGGAAGCCT GTGGTGGGCC AGTGTTCAGT CCACTCGCTC 
    AATGAGTTCT ACTTCCAGCC GGAAAGTCTG GAGAGAGACT CAACAGAAGA GGAATTTGAA 
    GTTATTGCTC ACACACCTAG AGATGATGTT CTGGTTGATA TTGATGACAA GGAGCCTCTA 
    ATCCCTGGAC AGGCAGATGG CACCAGCTCT GCCATTATTA ACATCTCGCC TTCTCAGTCC 
    AGCCTTCATC AGGATGAATT TATGGACTGG TGGAGCAAGT TTTATGCCTC CATGGGTGAG 
    AGGACCAAGT GTGGGAGTTA TCTGGAGAAA GGGTTCGATA TGCTGCAGGT GTATGACAGA 
    GAACTGGAGA AGACTGAAGG CTTTGAGCAA TTTGCTGATT TCTGTCAAAC GTTTAAACTT 
    TACCGTGGAA GAACCCAGAA TGACAGCGAA GATCCTTCCG TTGTTGGAGA GTTCAAGGGT 
    TCGTTTAAGA TCTATCCATT GCCTGATGAT CCCTCTATGC CTGTACCCAC ACGTCAGTTT 
    CGCAAACTCC CCCCGAACGG TATAGAGGAG TGCTTGGTAC GCATCTACAT CATCCAGGCT 
    TTTGGGTTAC AGCCCAAAGA TGCCAATGGC AAGTGTGACC CCTATGTTAA GATCACGCTT 
    GGCAAGAAAT CAGTCGATGA CCACGACAAT TACATACCCT GTACTTTGGA GCCCGTCTTT 
    GGAAAGATGT TTGAGCTGAC CTGCTGTCTG CCTCTTGAGA AAGACCTGAA GATTACACTC 
    TATGATTACG ACATGGTGAC TAAAGATGAG AAAATCGGAG AGACCATCAT TGATTTGGAG 
    AACCGCTTCT TGTCTCGTCA TGGTGCTCGC TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA 
    GGAATTAACC AGTGGCGAGA TCAACTTAAA CCATCTCAGC TACTTCATCG TCTGTGTGAG 
    AGGCGAAACT ACAAACTCCC CGTCTACAAG CAGGATCGAA TTTACTTCAG AGGACATGAA 
    TACACTGTCG CAGACCTTGA TGAAGGGAAA CCTCTTAACC CTCACTTGGG ACCAGTGATG 
    GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG CATGGTTTGG TGCCTGAACA TGTCGAGACG 
    CGACCCCTCT ACAGCCCCCT TCAGCCAGAT ATAGAACAGG GGCGACTACA AATGTGGGTG 
    GACCTGTTCC CTAAGTCTTT AGGCCCTCCA GGTCCTCCAT TCAACATCAC CCCACGCAAA 
    GCTAAAAAGT TCTTCCTGCG CTGTATTATA TGGAACACAA GAGATGTAAT CTTGGATGAT 
    GTCAGCGTTA CCGGGGAGAA GATGAGTGAC ATTTATGTGA AAGGCTGGAT GCACGGCCAT 
    GAGGAGAACA AGCAGAAAAC AGACGTGCAC TACCGATCGC TGGGTGGTGA GGGTAACTTT 
    AACTGGCGCT TCCTCTTCCC CTTCCACTAT TTACCTGCGG AACAACTGTG CACCATTGAT 
    AAAAAGGAGC ACTTTTGGAG TCTGGATAAA GTTGAAACGA AGCTTCCTCC TAAACTTACT 
    ATCCAGATTT GGGACAATGA CAAGTTCTCC TTTGATGATT TCCTCGGCTC TTTGGAGATG 
    GATCTGAACC ACATGCTGAG TCCTGCGAAG TCTCCAGAGA AGTGTGGTCT TGAGATGCTC 
    ACTCAGTCCC AGGACAAACT GGTCTCTTTG TTTGAGCAGA AAACGGTGAA AGGCTGGTGG 
    CCGTGTGTCT GTGACAAAGA AGACAAGAAG ATACTTGCTG GAAAGGTGGA GATGTCTCTG 
    GAAATAGTCT GTGAACAGGA GCAGGATGAG AGGCCTGCAG GACTCGGCCG AGATGAGCCC 
    AACATGAATC CCCATCTAGA GGAGCCTCAG CGTCCCGAGA CATCATTCTT ATGGTTCTCG 
    TCCCCATATA AGACGTTTAG GTTCATTGTG TGGAGGAGGT TTAAGTGGCT TATTCTGGGC 
    CTCTTTATAC TCTTTTTCAT TATTCTCTTC CTTGGAGTAT TTCTCTACGC CTTCCCTAAC 
    TATGCTGCTA TGAAGATGGT TGGACCCTTC AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333230.1
    CDS307..6663
    Translation

    Target ORF information:

    RefSeq Version XM_021477555.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021477555.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
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    2581
    2641
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    2761
    2821
    2881
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    3001
    3061
    3121
    3181
    3241
    3301
    3361
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    3481
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    3601
    3661
    3721
    3781
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    3901
    3961
    4021
    4081
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    4201
    4261
    4321
    4381
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    4561
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    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT 
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC 
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC 
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG 
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT 
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG 
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC 
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC 
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT 
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG 
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA 
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT 
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT 
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA 
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC 
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG 
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT 
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA 
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA 
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA 
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG 
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA 
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG 
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG 
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA 
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG 
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC 
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG 
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG 
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT 
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG 
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT 
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT 
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC 
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC 
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG 
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT 
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC 
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG 
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA 
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG 
    GATGAGCTTG TGCTTCCAGG GTTCCTCTCT GAGAATCAAC AGCAATGGCC AGAGGACTCT 
    GATTTGCCCT ATCCACCACC TCAGAGGGAG CCAAACGTCT ACATGGTGCC ACAGGGGATA 
    AAACCTGTCC TACAGCGCAC TGCTATTGAG ATACTGGCAT GGGGTTTGCG TAACCTGAAG 
    AGTTTCCAGC TGGCCAGTGT CACGTCTCCC AGTCTTCAGG TGGAATGTGG CGGATCTGTG 
    GTTCAGTCGT GCGTCATTCG AAATATTAAG AAGAACCCTA ATTTTGACGT CAACATCCTG 
    TTCATTGATG TCCGATTGCC TAAGGAAGAG CTCTACATGC CCCCATTGGT GATCAAGGTT 
    ATAGACAACC GGCAGTTTGG CAGGAAGCCT GTGGTGGGCC AGTGTTCAGT CCACTCGCTC 
    AATGAGTTCT ACTTCCAGCC GGAAAGTCTG GAGAGAGACT CAACAGAAGA GGAATTTGAA 
    GTTATTGCTC ACACACCTAG AGATGATGTT CTGGTTGATA TTGATGACAA GGAGCCTCTA 
    ATCCCTGGAC AGGCAGATGG CACCAGCTCT GCCATTATTA ACATCTCGCC TTCTCAGTCC 
    AGCCTTCATC AGGATGAATT TATGGACTGG TGGAGCAAGT TTTATGCCTC CATGGGTGAG 
    AGGACCAAGT GTGGGAGTTA TCTGGAGAAA GGGTTCGATA TGCTGCAGGT GTATGACAGA 
    GAACTGGAGA AGACTGAAGG CTTTGAGCAA TTTGCTGATT TCTGTCAAAC GTTTAAACTT 
    TACCGTGGAA GAACCCAGAA TGACAGCGAA GATCCTTCCG TTGTTGGAGA GTTCAAGGGT 
    TCGTTTAAGA TCTATCCATT GCCTGATGAT CCCTCTATGC CTGTACCCAC ACGTCAGTTT 
    CGCAAACTCC CCCCGAACGG TATAGAGGAG TGCTTGGTAC GCATCTACAT CATCCAGGCT 
    TTTGGGTTAC AGCCCAAAGA TGCCAATGGC AAGTGTGACC CCTATGTTAA GATCACGCTT 
    GGCAAGAAAT CAGTCGATGA CCACGACAAT TACATACCCT GTACTTTGGA GCCCGTCTTT 
    GGAAAGATGT TTGAGCTGAC CTGCTGTCTG CCTCTTGAGA AAGACCTGAA GATTACACTC 
    TATGATTACG ACATGGTGAC TAAAGATGAG AAAATCGGAG AGACCATCAT TGATTTGGAG 
    AACCGCTTCT TGTCTCGTCA TGGTGCTCGC TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA 
    GGAATTAACC AGTGGCGAGA TCAACTTAAA CCATCTCAGC TACTTCATCG TCTGTGTGAG 
    AGGCGAAACT ACAAACTCCC CGTCTACAAG CAGGATCGAA TTTACTTCAG AGGACATGAA 
    TACACTGTCG CAGACCTTGA TGAAGGGAAA CCTCTTAACC CTCACTTGGG ACCAGTGATG 
    GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG CATGGTTTGG TGCCTGAACA TGTCGAGACG 
    CGACCCCTCT ACAGCCCCCT TCAGCCAGAT ATAGAACAGG GGCGACTACA AATGTGGGTG 
    GACCTGTTCC CTAAGTCTTT AGGCCCTCCA GGTCCTCCAT TCAACATCAC CCCACGCAAA 
    GCTAAAAAGT TCTTCCTGCG CTGTATTATA TGGAACACAA GAGATGTAAT CTTGGATGAT 
    GTCAGCGTTA CCGGGGAGAA GATGAGTGAC ATTTATGTGA AAGGCTGGAT GCACGGCCAT 
    GAGGAGAACA AGCAGAAAAC AGACGTGCAC TACCGATCGC TGGGTGGTGA GGGTAACTTT 
    AACTGGCGCT TCCTCTTCCC CTTCCACTAT TTACCTGCGG AACAACTGTG CACCATTGAT 
    AAAAAGGAGC ACTTTTGGAG TCTGGATAAA GTTGAAACGA AGCTTCCTCC TAAACTTACT 
    ATCCAGATTT GGGACAATGA CAAGTTCTCC TTTGATGATT TCCTCGGCTC TTTGGAGATG 
    GATCTGAACC ACATGCTGAG TCCTGCGAAG TCTCCAGAGA AGTGTGGTCT TGAGATGCTC 
    ACTCAGTCCC AGGACAAACT GGTCTCTTTG TTTGAGCAGA AAACGGTGAA AGGCTGGTGG 
    CCGTGTGTCT GTGACAAAGA AGACAAGAAG ATACTTGCTG GAAAGGTGGA GATGTCTCTG 
    GAAATAGTCT GTGAACAGGA GCAGGATGAG AGGCCTGCAG GACTCGGCCG AGATGAGCCC 
    AACATGAATC CCCATCTAGA GGAGCCTCAG CGTCCCGAGA CATCATTCTT ATGGTTCTCG 
    TCCCCATATA AGACGTTTAG GTTCATTGTG TGGAGGAGGT TTAAGTGGCT TATTCTGGGC 
    CTCTTTATAC TCTTTTTCAT TATTCTCTTC CTTGGAGTAT TTCTCTACGC CTTCCCTAAC 
    TATGCTGCTA TGAAGATGGT TGGACCCTTC AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    dysf ( XM_021477556.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333231.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa143309
    Clone ID Related Accession (Same CDS sequence) XM_021477556.1
    Accession Version XM_021477556.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    1501
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    4681
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    4981
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    5101
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    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT 
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC 
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC 
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG 
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT 
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG 
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC 
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC 
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT 
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG 
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA 
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT 
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT 
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA 
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC 
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG 
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT 
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA 
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA 
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA 
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG 
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA 
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG 
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG 
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA 
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG 
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC 
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG 
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACGG 
    CCAGACGAGT CCGAGCGTGA GGGCTGGGAA TATGCCTCTT TATTTGGCTG GAAGTTTCAC 
    CTCAAGCAGA AGAAAACCGA CTTGTTCCGT CGCCGGCGCT GGAGACGTCG AATGGAGCCA 
    TTAGAAAAGA CGGGGCCGGC GGCCATCTTT GCCTTGGAGT GTTCGCTGAG CAGCTTTACT 
    GATGAAAAGA ATGACGATAA GTCAGTCACT ACAACATTTG GTGTCAGCCG GCCGACTATC 
    TCCTGCTTCT TTGATAGTGG GACCCTGTAT CATTTGCGCT GTTACTTGTA TCAGGCCAGA 
    GACCTGCTGG CTATGGACAA GGACAGTTTC TCGGATGCCT ATGCCGTTGT ATCGTTCCTT 
    CATCAGAGTC AGAAGACGGT GACTGTTCGA AACACACTCA ACCCCACCTG GGACCAGACC 
    CTCATTTTCT ACGAAGTGGA AATTTTTGGA GACCATCTTG TGACTGAGAG GAGCCCTCCA 
    AACATTGTGG TGGAGCTGTA CGATCAGGAC ACCTATGGAG CTGATGAGTT CATGGGCCGC 
    TGTGTTTGCC AACCATCAAT GGCATCTTCC CCTCGCCTCG CCTGGTATCC CATCCAGAAA 
    GCAGGGAATT CTGCAGGAGA TCTGCTTGCT GCTTTTGAAC TGATCCGCAG AGATAAACCT 
    GCAGTTCATC ACATCCCGGG TCAAGAGGGA GATATTATAC CACCCTCACA TATCATGGAT 
    GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG AATCAACAGC AATGGCCAGA GGACTCTGAT 
    TTGCCCTATC CACCACCTCA GAGGGAGCCA AACGTCTACA TGGTGCCACA GGGGATAAAA 
    CCTGTCCTAC AGCGCACTGC TATTGAGATA CTGGCATGGG GTTTGCGTAA CCTGAAGAGT 
    TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT CTTCAGGTGG AATGTGGCGG ATCTGTGGTT 
    CAGTCGTGCG TCATTCGAAA TATTAAGAAG AACCCTAATT TTGACGTCAA CATCCTGTTC 
    ATTGATGTCC GATTGCCTAA GGAAGAGCTC TACATGCCCC CATTGGTGAT CAAGGTTATA 
    GACAACCGGC AGTTTGGCAG GAAGCCTGTG GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT 
    GAGTTCTACT TCCAGCCGGA AAGTCTGGAG AGAGACTCAA CAGAAGAGGA ATTTGAAGTT 
    ATTGCTCACA CACCTAGAGA TGATGTTCTG GTTGATATTG ATGACAAGGA GCCTCTAATC 
    CCTGGACAGG CAGATGGCAC CAGCTCTGCC ATTATTAACA TCTCGCCTTC TCAGTCCAGC 
    CTTCATCAGG ATGAATTTAT GGACTGGTGG AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG 
    ACCAAGTGTG GGAGTTATCT GGAGAAAGGG TTCGATATGC TGCAGGTGTA TGACAGAGAA 
    CTGGAGAAGA CTGAAGGCTT TGAGCAATTT GCTGATTTCT GTCAAACGTT TAAACTTTAC 
    CGTGGAAGAA CCCAGAATGA CAGCGAAGAT CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG 
    TTTAAGATCT ATCCATTGCC TGATGATCCC TCTATGCCTG TACCCACACG TCAGTTTCGC 
    AAACTCCCCC CGAACGGTAT AGAGGAGTGC TTGGTACGCA TCTACATCAT CCAGGCTTTT 
    GGGTTACAGC CCAAAGATGC CAATGGCAAG TGTGACCCCT ATGTTAAGAT CACGCTTGGC 
    AAGAAATCAG TCGATGACCA CGACAATTAC ATACCCTGTA CTTTGGAGCC CGTCTTTGGA 
    AAGATGTTTG AGCTGACCTG CTGTCTGCCT CTTGAGAAAG ACCTGAAGAT TACACTCTAT 
    GATTACGACA TGGTGACTAA AGATGAGAAA ATCGGAGAGA CCATCATTGA TTTGGAGAAC 
    CGCTTCTTGT CTCGTCATGG TGCTCGCTGC GGCCTTCCGC AGTCCTACTG TCTGTCAGGA 
    ATTAACCAGT GGCGAGATCA ACTTAAACCA TCTCAGCTAC TTCATCGTCT GTGTGAGAGG 
    CGAAACTACA AACTCCCCGT CTACAAGCAG GATCGAATTT ACTTCAGAGG ACATGAATAC 
    ACTGTCGCAG ACCTTGATGA AGGGAAACCT CTTAACCCTC ACTTGGGACC AGTGATGGAG 
    CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT GGTTTGGTGC CTGAACATGT CGAGACGCGA 
    CCCCTCTACA GCCCCCTTCA GCCAGATATA GAACAGGGGC GACTACAAAT GTGGGTGGAC 
    CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT CCTCCATTCA ACATCACCCC ACGCAAAGCT 
    AAAAAGTTCT TCCTGCGCTG TATTATATGG AACACAAGAG ATGTAATCTT GGATGATGTC 
    AGCGTTACCG GGGAGAAGAT GAGTGACATT TATGTGAAAG GCTGGATGCA CGGCCATGAG 
    GAGAACAAGC AGAAAACAGA CGTGCACTAC CGATCGCTGG GTGGTGAGGG TAACTTTAAC 
    TGGCGCTTCC TCTTCCCCTT CCACTATTTA CCTGCGGAAC AACTGTGCAC CATTGATAAA 
    AAGGAGCACT TTTGGAGTCT GGATAAAGTT GAAACGAAGC TTCCTCCTAA ACTTACTATC 
    CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGATTTCC TCGGCTCTTT GGAGATGGAT 
    CTGAACCACA TGCTGAGTCC TGCGAAGTCT CCAGAGAAGT GTGGTCTTGA GATGCTCACT 
    CAGTCCCAGG ACAAACTGGT CTCTTTGTTT GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG 
    TGTGTCTGTG ACAAAGAAGA CAAGAAGATA CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA 
    ATAGTCTGTG AACAGGAGCA GGATGAGAGG CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC 
    ATGAATCCCC ATCTAGAGGA GCCTCAGCGT CCCGAGACAT CATTCTTATG GTTCTCGTCC 
    CCATATAAGA CGTTTAGGTT CATTGTGTGG AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC 
    TTTATACTCT TTTTCATTAT TCTCTTCCTT GGAGTATTTC TCTACGCCTT CCCTAACTAT 
    GCTGCTATGA AGATGGTTGG ACCCTTCAGT GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333231.1
    CDS307..6660
    Translation

    Target ORF information:

    RefSeq Version XM_021477556.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021477556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT 
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC 
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC 
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG 
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT 
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG 
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC 
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC 
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT 
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG 
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA 
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT 
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT 
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA 
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC 
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG 
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT 
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA 
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA 
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA 
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG 
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA 
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG 
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG 
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA 
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG 
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC 
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG 
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACGG 
    CCAGACGAGT CCGAGCGTGA GGGCTGGGAA TATGCCTCTT TATTTGGCTG GAAGTTTCAC 
    CTCAAGCAGA AGAAAACCGA CTTGTTCCGT CGCCGGCGCT GGAGACGTCG AATGGAGCCA 
    TTAGAAAAGA CGGGGCCGGC GGCCATCTTT GCCTTGGAGT GTTCGCTGAG CAGCTTTACT 
    GATGAAAAGA ATGACGATAA GTCAGTCACT ACAACATTTG GTGTCAGCCG GCCGACTATC 
    TCCTGCTTCT TTGATAGTGG GACCCTGTAT CATTTGCGCT GTTACTTGTA TCAGGCCAGA 
    GACCTGCTGG CTATGGACAA GGACAGTTTC TCGGATGCCT ATGCCGTTGT ATCGTTCCTT 
    CATCAGAGTC AGAAGACGGT GACTGTTCGA AACACACTCA ACCCCACCTG GGACCAGACC 
    CTCATTTTCT ACGAAGTGGA AATTTTTGGA GACCATCTTG TGACTGAGAG GAGCCCTCCA 
    AACATTGTGG TGGAGCTGTA CGATCAGGAC ACCTATGGAG CTGATGAGTT CATGGGCCGC 
    TGTGTTTGCC AACCATCAAT GGCATCTTCC CCTCGCCTCG CCTGGTATCC CATCCAGAAA 
    GCAGGGAATT CTGCAGGAGA TCTGCTTGCT GCTTTTGAAC TGATCCGCAG AGATAAACCT 
    GCAGTTCATC ACATCCCGGG TCAAGAGGGA GATATTATAC CACCCTCACA TATCATGGAT 
    GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG AATCAACAGC AATGGCCAGA GGACTCTGAT 
    TTGCCCTATC CACCACCTCA GAGGGAGCCA AACGTCTACA TGGTGCCACA GGGGATAAAA 
    CCTGTCCTAC AGCGCACTGC TATTGAGATA CTGGCATGGG GTTTGCGTAA CCTGAAGAGT 
    TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT CTTCAGGTGG AATGTGGCGG ATCTGTGGTT 
    CAGTCGTGCG TCATTCGAAA TATTAAGAAG AACCCTAATT TTGACGTCAA CATCCTGTTC 
    ATTGATGTCC GATTGCCTAA GGAAGAGCTC TACATGCCCC CATTGGTGAT CAAGGTTATA 
    GACAACCGGC AGTTTGGCAG GAAGCCTGTG GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT 
    GAGTTCTACT TCCAGCCGGA AAGTCTGGAG AGAGACTCAA CAGAAGAGGA ATTTGAAGTT 
    ATTGCTCACA CACCTAGAGA TGATGTTCTG GTTGATATTG ATGACAAGGA GCCTCTAATC 
    CCTGGACAGG CAGATGGCAC CAGCTCTGCC ATTATTAACA TCTCGCCTTC TCAGTCCAGC 
    CTTCATCAGG ATGAATTTAT GGACTGGTGG AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG 
    ACCAAGTGTG GGAGTTATCT GGAGAAAGGG TTCGATATGC TGCAGGTGTA TGACAGAGAA 
    CTGGAGAAGA CTGAAGGCTT TGAGCAATTT GCTGATTTCT GTCAAACGTT TAAACTTTAC 
    CGTGGAAGAA CCCAGAATGA CAGCGAAGAT CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG 
    TTTAAGATCT ATCCATTGCC TGATGATCCC TCTATGCCTG TACCCACACG TCAGTTTCGC 
    AAACTCCCCC CGAACGGTAT AGAGGAGTGC TTGGTACGCA TCTACATCAT CCAGGCTTTT 
    GGGTTACAGC CCAAAGATGC CAATGGCAAG TGTGACCCCT ATGTTAAGAT CACGCTTGGC 
    AAGAAATCAG TCGATGACCA CGACAATTAC ATACCCTGTA CTTTGGAGCC CGTCTTTGGA 
    AAGATGTTTG AGCTGACCTG CTGTCTGCCT CTTGAGAAAG ACCTGAAGAT TACACTCTAT 
    GATTACGACA TGGTGACTAA AGATGAGAAA ATCGGAGAGA CCATCATTGA TTTGGAGAAC 
    CGCTTCTTGT CTCGTCATGG TGCTCGCTGC GGCCTTCCGC AGTCCTACTG TCTGTCAGGA 
    ATTAACCAGT GGCGAGATCA ACTTAAACCA TCTCAGCTAC TTCATCGTCT GTGTGAGAGG 
    CGAAACTACA AACTCCCCGT CTACAAGCAG GATCGAATTT ACTTCAGAGG ACATGAATAC 
    ACTGTCGCAG ACCTTGATGA AGGGAAACCT CTTAACCCTC ACTTGGGACC AGTGATGGAG 
    CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT GGTTTGGTGC CTGAACATGT CGAGACGCGA 
    CCCCTCTACA GCCCCCTTCA GCCAGATATA GAACAGGGGC GACTACAAAT GTGGGTGGAC 
    CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT CCTCCATTCA ACATCACCCC ACGCAAAGCT 
    AAAAAGTTCT TCCTGCGCTG TATTATATGG AACACAAGAG ATGTAATCTT GGATGATGTC 
    AGCGTTACCG GGGAGAAGAT GAGTGACATT TATGTGAAAG GCTGGATGCA CGGCCATGAG 
    GAGAACAAGC AGAAAACAGA CGTGCACTAC CGATCGCTGG GTGGTGAGGG TAACTTTAAC 
    TGGCGCTTCC TCTTCCCCTT CCACTATTTA CCTGCGGAAC AACTGTGCAC CATTGATAAA 
    AAGGAGCACT TTTGGAGTCT GGATAAAGTT GAAACGAAGC TTCCTCCTAA ACTTACTATC 
    CAGATTTGGG ACAATGACAA GTTCTCCTTT GATGATTTCC TCGGCTCTTT GGAGATGGAT 
    CTGAACCACA TGCTGAGTCC TGCGAAGTCT CCAGAGAAGT GTGGTCTTGA GATGCTCACT 
    CAGTCCCAGG ACAAACTGGT CTCTTTGTTT GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG 
    TGTGTCTGTG ACAAAGAAGA CAAGAAGATA CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA 
    ATAGTCTGTG AACAGGAGCA GGATGAGAGG CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC 
    ATGAATCCCC ATCTAGAGGA GCCTCAGCGT CCCGAGACAT CATTCTTATG GTTCTCGTCC 
    CCATATAAGA CGTTTAGGTT CATTGTGTGG AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC 
    TTTATACTCT TTTTCATTAT TCTCTTCCTT GGAGTATTTC TCTACGCCTT CCCTAACTAT 
    GCTGCTATGA AGATGGTTGG ACCCTTCAGT GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    dysf ( XM_021477557.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333232.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa143310
    Clone ID Related Accession (Same CDS sequence) XM_021477557.1
    Accession Version XM_021477557.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG 
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT 
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG 
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG 
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG 
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC 
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT 
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT 
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT 
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC 
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG 
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC 
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG 
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA 
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA 
    AACAGTCTGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC 
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA 
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT 
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT 
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG 
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA 
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG 
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG 
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG 
    GGGTACACAG ATGTGAATGG AGAAAAAGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT 
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGT GAGGATCTGA AGCGTGCAGT GGATGATCAA 
    GGGTGGGAAT ATGGAATAAC CCTTCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT 
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG 
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA 
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT 
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CGGGGCCGGC GGCCATCTTT 
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT 
    ACAACATTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT 
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC 
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTT CATCAGAGTC AGAAGACGGT GACTGTTCGA 
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA 
    GACCATCTTG TGACTGAGAG GAGCCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC 
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC 
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT 
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA 
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG 
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA 
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA 
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT 
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG 
    AACCCTAATT TTGACGTCAA CATCCTGTTC ATTGATGTCC GATTGCCTAA GGAAGAGCTC 
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG 
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG 
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG 
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGG CAGATGGCAC CAGCTCTGCC 
    ATTATTAACA TCTCGCCTTC TCAGTCCAGC CTTCATCAGG ATGAATTTAT GGACTGGTGG 
    AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG ACCAAGTGTG GGAGTTATCT GGAGAAAGGG 
    TTCGATATGC TGCAGGTGTA TGACAGAGAA CTGGAGAAGA CTGAAGGCTT TGAGCAATTT 
    GCTGATTTCT GTCAAACGTT TAAACTTTAC CGTGGAAGAA CCCAGAATGA CAGCGAAGAT 
    CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG TTTAAGATCT ATCCATTGCC TGATGATCCC 
    TCTATGCCTG TACCCACACG TCAGTTTCGC AAACTCCCCC CGAACGGTAT AGAGGAGTGC 
    TTGGTACGCA TCTACATCAT CCAGGCTTTT GGGTTACAGC CCAAAGATGC CAATGGCAAG 
    TGTGACCCCT ATGTTAAGAT CACGCTTGGC AAGAAATCAG TCGATGACCA CGACAATTAC 
    ATACCCTGTA CTTTGGAGCC CGTCTTTGGA AAGATGTTTG AGCTGACCTG CTGTCTGCCT 
    CTTGAGAAAG ACCTGAAGAT TACACTCTAT GATTACGACA TGGTGACTAA AGATGAGAAA 
    ATCGGAGAGA CCATCATTGA TTTGGAGAAC CGCTTCTTGT CTCGTCATGG TGCTCGCTGC 
    GGCCTTCCGC AGTCCTACTG TCTGTCAGGA ATTAACCAGT GGCGAGATCA ACTTAAACCA 
    TCTCAGCTAC TTCATCGTCT GTGTGAGAGG CGAAACTACA AACTCCCCGT CTACAAGCAG 
    GATCGAATTT ACTTCAGAGG ACATGAATAC ACTGTCGCAG ACCTTGATGA AGGGAAACCT 
    CTTAACCCTC ACTTGGGACC AGTGATGGAG CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT 
    GGTTTGGTGC CTGAACATGT CGAGACGCGA CCCCTCTACA GCCCCCTTCA GCCAGATATA 
    GAACAGGGGC GACTACAAAT GTGGGTGGAC CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT 
    CCTCCATTCA ACATCACCCC ACGCAAAGCT AAAAAGTTCT TCCTGCGCTG TATTATATGG 
    AACACAAGAG ATGTAATCTT GGATGATGTC AGCGTTACCG GGGAGAAGAT GAGTGACATT 
    TATGTGAAAG GCTGGATGCA CGGCCATGAG GAGAACAAGC AGAAAACAGA CGTGCACTAC 
    CGATCGCTGG GTGGTGAGGG TAACTTTAAC TGGCGCTTCC TCTTCCCCTT CCACTATTTA 
    CCTGCGGAAC AACTGTGCAC CATTGATAAA AAGGAGCACT TTTGGAGTCT GGATAAAGTT 
    GAAACGAAGC TTCCTCCTAA ACTTACTATC CAGATTTGGG ACAATGACAA GTTCTCCTTT 
    GATGATTTCC TCGGCTCTTT GGAGATGGAT CTGAACCACA TGCTGAGTCC TGCGAAGTCT 
    CCAGAGAAGT GTGGTCTTGA GATGCTCACT CAGTCCCAGG ACAAACTGGT CTCTTTGTTT 
    GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG TGTGTCTGTG ACAAAGAAGA CAAGAAGATA 
    CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA ATAGTCTGTG AACAGGAGCA GGATGAGAGG 
    CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC ATGAATCCCC ATCTAGAGGA GCCTCAGCGT 
    CCCGAGACAT CATTCTTATG GTTCTCGTCC CCATATAAGA CGTTTAGGTT CATTGTGTGG 
    AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC TTTATACTCT TTTTCATTAT TCTCTTCCTT 
    GGAGTATTTC TCTACGCCTT CCCTAACTAT GCTGCTATGA AGATGGTTGG ACCCTTCAGT 
    GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333232.1
    CDS307..6630
    Translation

    Target ORF information:

    RefSeq Version XM_021477557.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021477557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG 
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT 
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG 
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG 
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG 
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC 
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT 
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT 
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT 
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC 
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG 
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC 
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG 
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA 
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA 
    AACAGTCTGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC 
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA 
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT 
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT 
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG 
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA 
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG 
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG 
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG 
    GGGTACACAG ATGTGAATGG AGAAAAAGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT 
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGT GAGGATCTGA AGCGTGCAGT GGATGATCAA 
    GGGTGGGAAT ATGGAATAAC CCTTCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT 
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG 
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA 
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT 
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CGGGGCCGGC GGCCATCTTT 
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT 
    ACAACATTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT 
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC 
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTT CATCAGAGTC AGAAGACGGT GACTGTTCGA 
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA 
    GACCATCTTG TGACTGAGAG GAGCCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC 
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC 
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT 
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA 
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG 
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA 
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA 
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT 
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG 
    AACCCTAATT TTGACGTCAA CATCCTGTTC ATTGATGTCC GATTGCCTAA GGAAGAGCTC 
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG 
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG 
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG 
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGG CAGATGGCAC CAGCTCTGCC 
    ATTATTAACA TCTCGCCTTC TCAGTCCAGC CTTCATCAGG ATGAATTTAT GGACTGGTGG 
    AGCAAGTTTT ATGCCTCCAT GGGTGAGAGG ACCAAGTGTG GGAGTTATCT GGAGAAAGGG 
    TTCGATATGC TGCAGGTGTA TGACAGAGAA CTGGAGAAGA CTGAAGGCTT TGAGCAATTT 
    GCTGATTTCT GTCAAACGTT TAAACTTTAC CGTGGAAGAA CCCAGAATGA CAGCGAAGAT 
    CCTTCCGTTG TTGGAGAGTT CAAGGGTTCG TTTAAGATCT ATCCATTGCC TGATGATCCC 
    TCTATGCCTG TACCCACACG TCAGTTTCGC AAACTCCCCC CGAACGGTAT AGAGGAGTGC 
    TTGGTACGCA TCTACATCAT CCAGGCTTTT GGGTTACAGC CCAAAGATGC CAATGGCAAG 
    TGTGACCCCT ATGTTAAGAT CACGCTTGGC AAGAAATCAG TCGATGACCA CGACAATTAC 
    ATACCCTGTA CTTTGGAGCC CGTCTTTGGA AAGATGTTTG AGCTGACCTG CTGTCTGCCT 
    CTTGAGAAAG ACCTGAAGAT TACACTCTAT GATTACGACA TGGTGACTAA AGATGAGAAA 
    ATCGGAGAGA CCATCATTGA TTTGGAGAAC CGCTTCTTGT CTCGTCATGG TGCTCGCTGC 
    GGCCTTCCGC AGTCCTACTG TCTGTCAGGA ATTAACCAGT GGCGAGATCA ACTTAAACCA 
    TCTCAGCTAC TTCATCGTCT GTGTGAGAGG CGAAACTACA AACTCCCCGT CTACAAGCAG 
    GATCGAATTT ACTTCAGAGG ACATGAATAC ACTGTCGCAG ACCTTGATGA AGGGAAACCT 
    CTTAACCCTC ACTTGGGACC AGTGATGGAG CGTCTGGCTC TGTTGGTGTT GAGGAGGCAT 
    GGTTTGGTGC CTGAACATGT CGAGACGCGA CCCCTCTACA GCCCCCTTCA GCCAGATATA 
    GAACAGGGGC GACTACAAAT GTGGGTGGAC CTGTTCCCTA AGTCTTTAGG CCCTCCAGGT 
    CCTCCATTCA ACATCACCCC ACGCAAAGCT AAAAAGTTCT TCCTGCGCTG TATTATATGG 
    AACACAAGAG ATGTAATCTT GGATGATGTC AGCGTTACCG GGGAGAAGAT GAGTGACATT 
    TATGTGAAAG GCTGGATGCA CGGCCATGAG GAGAACAAGC AGAAAACAGA CGTGCACTAC 
    CGATCGCTGG GTGGTGAGGG TAACTTTAAC TGGCGCTTCC TCTTCCCCTT CCACTATTTA 
    CCTGCGGAAC AACTGTGCAC CATTGATAAA AAGGAGCACT TTTGGAGTCT GGATAAAGTT 
    GAAACGAAGC TTCCTCCTAA ACTTACTATC CAGATTTGGG ACAATGACAA GTTCTCCTTT 
    GATGATTTCC TCGGCTCTTT GGAGATGGAT CTGAACCACA TGCTGAGTCC TGCGAAGTCT 
    CCAGAGAAGT GTGGTCTTGA GATGCTCACT CAGTCCCAGG ACAAACTGGT CTCTTTGTTT 
    GAGCAGAAAA CGGTGAAAGG CTGGTGGCCG TGTGTCTGTG ACAAAGAAGA CAAGAAGATA 
    CTTGCTGGAA AGGTGGAGAT GTCTCTGGAA ATAGTCTGTG AACAGGAGCA GGATGAGAGG 
    CCTGCAGGAC TCGGCCGAGA TGAGCCCAAC ATGAATCCCC ATCTAGAGGA GCCTCAGCGT 
    CCCGAGACAT CATTCTTATG GTTCTCGTCC CCATATAAGA CGTTTAGGTT CATTGTGTGG 
    AGGAGGTTTA AGTGGCTTAT TCTGGGCCTC TTTATACTCT TTTTCATTAT TCTCTTCCTT 
    GGAGTATTTC TCTACGCCTT CCCTAACTAT GCTGCTATGA AGATGGTTGG ACCCTTCAGT 
    GGACTAGGAA AGGGAACACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    dysf ( XM_021477558.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333233.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa143311
    Clone ID Related Accession (Same CDS sequence) XM_021477558.1
    Accession Version XM_021477558.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT 
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC 
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC 
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG 
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT 
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG 
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC 
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC 
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT 
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG 
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA 
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT 
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT 
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA 
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC 
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG 
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT 
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA 
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA 
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA 
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG 
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA 
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG 
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG 
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA 
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG 
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC 
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG 
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG 
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT 
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG 
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT 
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT 
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC 
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC 
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG 
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT 
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC 
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG 
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA 
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG 
    GATGAGCCAG AGGACTCTGA TTTGCCCTAT CCACCACCTC AGAGGGAGCC AAACGTCTAC 
    ATGGTGCCAC AGGGGATAAA ACCTGTCCTA CAGCGCACTG CTATTGAGAT ACTGGCATGG 
    GGTTTGCGTA ACCTGAAGAG TTTCCAGCTG GCCAGTGTCA CGTCTCCCAG TCTTCAGGTG 
    GAATGTGGCG GATCTGTGGT TCAGTCGTGC GTCATTCGAA ATATTAAGAA GAACCCTAAT 
    TTTGACGTCA ACATCCTGTT CATTGATGTC CGATTGCCTA AGGAAGAGCT CTACATGCCC 
    CCATTGGTGA TCAAGGTTAT AGACAACCGG CAGTTTGGCA GGAAGCCTGT GGTGGGCCAG 
    TGTTCAGTCC ACTCGCTCAA TGAGTTCTAC TTCCAGCCGG AAAGTCTGGA GAGAGACTCA 
    ACAGAAGAGG AATTTGAAGT TATTGCTCAC ACACCTAGAG ATGATGTTCT GGTTGATATT 
    GATGACAAGG AGCCTCTAAT CCCTGGACAG GCAGATGGCA CCAGCTCTGC CATTATTAAC 
    ATCTCGCCTT CTCAGTCCAG CCTTCATCAG GATGAATTTA TGGACTGGTG GAGCAAGTTT 
    TATGCCTCCA TGGGTGAGAG GACCAAGTGT GGGAGTTATC TGGAGAAAGG GTTCGATATG 
    CTGCAGGTGT ATGACAGAGA ACTGGAGAAG ACTGAAGGCT TTGAGCAATT TGCTGATTTC 
    TGTCAAACGT TTAAACTTTA CCGTGGAAGA ACCCAGAATG ACAGCGAAGA TCCTTCCGTT 
    GTTGGAGAGT TCAAGGGTTC GTTTAAGATC TATCCATTGC CTGATGATCC CTCTATGCCT 
    GTACCCACAC GTCAGTTTCG CAAACTCCCC CCGAACGGTA TAGAGGAGTG CTTGGTACGC 
    ATCTACATCA TCCAGGCTTT TGGGTTACAG CCCAAAGATG CCAATGGCAA GTGTGACCCC 
    TATGTTAAGA TCACGCTTGG CAAGAAATCA GTCGATGACC ACGACAATTA CATACCCTGT 
    ACTTTGGAGC CCGTCTTTGG AAAGATGTTT GAGCTGACCT GCTGTCTGCC TCTTGAGAAA 
    GACCTGAAGA TTACACTCTA TGATTACGAC ATGGTGACTA AAGATGAGAA AATCGGAGAG 
    ACCATCATTG ATTTGGAGAA CCGCTTCTTG TCTCGTCATG GTGCTCGCTG CGGCCTTCCG 
    CAGTCCTACT GTCTGTCAGG AATTAACCAG TGGCGAGATC AACTTAAACC ATCTCAGCTA 
    CTTCATCGTC TGTGTGAGAG GCGAAACTAC AAACTCCCCG TCTACAAGCA GGATCGAATT 
    TACTTCAGAG GACATGAATA CACTGTCGCA GACCTTGATG AAGGGAAACC TCTTAACCCT 
    CACTTGGGAC CAGTGATGGA GCGTCTGGCT CTGTTGGTGT TGAGGAGGCA TGGTTTGGTG 
    CCTGAACATG TCGAGACGCG ACCCCTCTAC AGCCCCCTTC AGCCAGATAT AGAACAGGGG 
    CGACTACAAA TGTGGGTGGA CCTGTTCCCT AAGTCTTTAG GCCCTCCAGG TCCTCCATTC 
    AACATCACCC CACGCAAAGC TAAAAAGTTC TTCCTGCGCT GTATTATATG GAACACAAGA 
    GATGTAATCT TGGATGATGT CAGCGTTACC GGGGAGAAGA TGAGTGACAT TTATGTGAAA 
    GGCTGGATGC ACGGCCATGA GGAGAACAAG CAGAAAACAG ACGTGCACTA CCGATCGCTG 
    GGTGGTGAGG GTAACTTTAA CTGGCGCTTC CTCTTCCCCT TCCACTATTT ACCTGCGGAA 
    CAACTGTGCA CCATTGATAA AAAGGAGCAC TTTTGGAGTC TGGATAAAGT TGAAACGAAG 
    CTTCCTCCTA AACTTACTAT CCAGATTTGG GACAATGACA AGTTCTCCTT TGATGATTTC 
    CTCGGCTCTT TGGAGATGGA TCTGAACCAC ATGCTGAGTC CTGCGAAGTC TCCAGAGAAG 
    TGTGGTCTTG AGATGCTCAC TCAGTCCCAG GACAAACTGG TCTCTTTGTT TGAGCAGAAA 
    ACGGTGAAAG GCTGGTGGCC GTGTGTCTGT GACAAAGAAG ACAAGAAGAT ACTTGCTGGA 
    AAGGTGGAGA TGTCTCTGGA AATAGTCTGT GAACAGGAGC AGGATGAGAG GCCTGCAGGA 
    CTCGGCCGAG ATGAGCCCAA CATGAATCCC CATCTAGAGG AGCCTCAGCG TCCCGAGACA 
    TCATTCTTAT GGTTCTCGTC CCCATATAAG ACGTTTAGGT TCATTGTGTG GAGGAGGTTT 
    AAGTGGCTTA TTCTGGGCCT CTTTATACTC TTTTTCATTA TTCTCTTCCT TGGAGTATTT 
    CTCTACGCCT TCCCTAACTA TGCTGCTATG AAGATGGTTG GACCCTTCAG TGGACTAGGA 
    AAGGGAACAC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333233.1
    CDS307..6621
    Translation

    Target ORF information:

    RefSeq Version XM_021477558.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021477558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT 
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC 
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC 
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG 
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT 
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG 
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC 
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC 
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT 
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG 
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA 
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT 
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT 
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA 
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC 
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG 
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT 
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA 
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA 
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA 
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG 
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA 
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG 
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG 
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA 
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG 
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC 
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG 
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG 
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT 
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG 
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT 
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT 
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC 
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC 
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG 
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT 
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC 
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG 
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA 
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG 
    GATGAGCCAG AGGACTCTGA TTTGCCCTAT CCACCACCTC AGAGGGAGCC AAACGTCTAC 
    ATGGTGCCAC AGGGGATAAA ACCTGTCCTA CAGCGCACTG CTATTGAGAT ACTGGCATGG 
    GGTTTGCGTA ACCTGAAGAG TTTCCAGCTG GCCAGTGTCA CGTCTCCCAG TCTTCAGGTG 
    GAATGTGGCG GATCTGTGGT TCAGTCGTGC GTCATTCGAA ATATTAAGAA GAACCCTAAT 
    TTTGACGTCA ACATCCTGTT CATTGATGTC CGATTGCCTA AGGAAGAGCT CTACATGCCC 
    CCATTGGTGA TCAAGGTTAT AGACAACCGG CAGTTTGGCA GGAAGCCTGT GGTGGGCCAG 
    TGTTCAGTCC ACTCGCTCAA TGAGTTCTAC TTCCAGCCGG AAAGTCTGGA GAGAGACTCA 
    ACAGAAGAGG AATTTGAAGT TATTGCTCAC ACACCTAGAG ATGATGTTCT GGTTGATATT 
    GATGACAAGG AGCCTCTAAT CCCTGGACAG GCAGATGGCA CCAGCTCTGC CATTATTAAC 
    ATCTCGCCTT CTCAGTCCAG CCTTCATCAG GATGAATTTA TGGACTGGTG GAGCAAGTTT 
    TATGCCTCCA TGGGTGAGAG GACCAAGTGT GGGAGTTATC TGGAGAAAGG GTTCGATATG 
    CTGCAGGTGT ATGACAGAGA ACTGGAGAAG ACTGAAGGCT TTGAGCAATT TGCTGATTTC 
    TGTCAAACGT TTAAACTTTA CCGTGGAAGA ACCCAGAATG ACAGCGAAGA TCCTTCCGTT 
    GTTGGAGAGT TCAAGGGTTC GTTTAAGATC TATCCATTGC CTGATGATCC CTCTATGCCT 
    GTACCCACAC GTCAGTTTCG CAAACTCCCC CCGAACGGTA TAGAGGAGTG CTTGGTACGC 
    ATCTACATCA TCCAGGCTTT TGGGTTACAG CCCAAAGATG CCAATGGCAA GTGTGACCCC 
    TATGTTAAGA TCACGCTTGG CAAGAAATCA GTCGATGACC ACGACAATTA CATACCCTGT 
    ACTTTGGAGC CCGTCTTTGG AAAGATGTTT GAGCTGACCT GCTGTCTGCC TCTTGAGAAA 
    GACCTGAAGA TTACACTCTA TGATTACGAC ATGGTGACTA AAGATGAGAA AATCGGAGAG 
    ACCATCATTG ATTTGGAGAA CCGCTTCTTG TCTCGTCATG GTGCTCGCTG CGGCCTTCCG 
    CAGTCCTACT GTCTGTCAGG AATTAACCAG TGGCGAGATC AACTTAAACC ATCTCAGCTA 
    CTTCATCGTC TGTGTGAGAG GCGAAACTAC AAACTCCCCG TCTACAAGCA GGATCGAATT 
    TACTTCAGAG GACATGAATA CACTGTCGCA GACCTTGATG AAGGGAAACC TCTTAACCCT 
    CACTTGGGAC CAGTGATGGA GCGTCTGGCT CTGTTGGTGT TGAGGAGGCA TGGTTTGGTG 
    CCTGAACATG TCGAGACGCG ACCCCTCTAC AGCCCCCTTC AGCCAGATAT AGAACAGGGG 
    CGACTACAAA TGTGGGTGGA CCTGTTCCCT AAGTCTTTAG GCCCTCCAGG TCCTCCATTC 
    AACATCACCC CACGCAAAGC TAAAAAGTTC TTCCTGCGCT GTATTATATG GAACACAAGA 
    GATGTAATCT TGGATGATGT CAGCGTTACC GGGGAGAAGA TGAGTGACAT TTATGTGAAA 
    GGCTGGATGC ACGGCCATGA GGAGAACAAG CAGAAAACAG ACGTGCACTA CCGATCGCTG 
    GGTGGTGAGG GTAACTTTAA CTGGCGCTTC CTCTTCCCCT TCCACTATTT ACCTGCGGAA 
    CAACTGTGCA CCATTGATAA AAAGGAGCAC TTTTGGAGTC TGGATAAAGT TGAAACGAAG 
    CTTCCTCCTA AACTTACTAT CCAGATTTGG GACAATGACA AGTTCTCCTT TGATGATTTC 
    CTCGGCTCTT TGGAGATGGA TCTGAACCAC ATGCTGAGTC CTGCGAAGTC TCCAGAGAAG 
    TGTGGTCTTG AGATGCTCAC TCAGTCCCAG GACAAACTGG TCTCTTTGTT TGAGCAGAAA 
    ACGGTGAAAG GCTGGTGGCC GTGTGTCTGT GACAAAGAAG ACAAGAAGAT ACTTGCTGGA 
    AAGGTGGAGA TGTCTCTGGA AATAGTCTGT GAACAGGAGC AGGATGAGAG GCCTGCAGGA 
    CTCGGCCGAG ATGAGCCCAA CATGAATCCC CATCTAGAGG AGCCTCAGCG TCCCGAGACA 
    TCATTCTTAT GGTTCTCGTC CCCATATAAG ACGTTTAGGT TCATTGTGTG GAGGAGGTTT 
    AAGTGGCTTA TTCTGGGCCT CTTTATACTC TTTTTCATTA TTCTCTTCCT TGGAGTATTT 
    CTCTACGCCT TCCCTAACTA TGCTGCTATG AAGATGGTTG GACCCTTCAG TGGACTAGGA 
    AAGGGAACAC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    dysf ( XM_021477560.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333235.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID ODa143312
    Clone ID Related Accession (Same CDS sequence) XM_021477560.1
    Accession Version XM_021477560.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT 
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC 
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC 
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG 
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT 
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG 
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC 
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC 
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT 
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG 
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA 
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT 
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT 
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA 
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC 
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG 
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT 
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA 
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA 
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA 
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG 
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA 
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG 
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG 
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA 
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG 
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC 
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG 
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG 
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT 
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG 
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT 
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT 
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC 
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC 
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG 
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT 
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC 
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG 
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA 
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG 
    GATGAGCTTG TGCTTCCAGG GTTCCTCTCT GAGAATCAAC AGCAATGGCC AGAGGACTCT 
    GATTTGCCCT ATCCACCACC TCAGAGGGAG CCAAACGTCT ACATGGTGCC ACAGGGGATA 
    AAACCTGTCC TACAGCGCAC TGCTATTGAG ATACTGGCAT GGGGTTTGCG TAACCTGAAG 
    AGTTTCCAGC TGGCCAGTGT CACGTCTCCC AGTCTTCAGG TGGAATGTGG CGGATCTGTG 
    GTTCAGTCGT GCGTCATTCG AAATATTAAG AAGAACCCTA ATTTTGACGT CAACATCCTG 
    TTCATTGATG TCCGATTGCC TAAGGAAGAG CTCTACATGC CCCCATTGGT GATCAAGGTT 
    ATAGACAACC GGCAGTTTGG CAGGAAGCCT GTGGTGGGCC AGTGTTCAGT CCACTCGCTC 
    AATGAGTTCT ACTTCCAGCC GGAAAGTCTG GAGAGAGACT CAACAGAAGA GGAATTTGAA 
    GTTATTGCTC ACACACCTAG AGATGATGTT CTGGTTGATA TTGATGACAA GGAGCCTCTA 
    ATCCCTGGAC AGGATGAATT TATGGACTGG TGGAGCAAGT TTTATGCCTC CATGGGTGAG 
    AGGACCAAGT GTGGGAGTTA TCTGGAGAAA GGGTTCGATA TGCTGCAGGT GTATGACAGA 
    GAACTGGAGA AGACTGAAGG CTTTGAGCAA TTTGCTGATT TCTGTCAAAC GTTTAAACTT 
    TACCGTGGAA GAACCCAGAA TGACAGCGAA GATCCTTCCG TTGTTGGAGA GTTCAAGGGT 
    TCGTTTAAGA TCTATCCATT GCCTGATGAT CCCTCTATGC CTGTACCCAC ACGTCAGTTT 
    CGCAAACTCC CCCCGAACGG TATAGAGGAG TGCTTGGTAC GCATCTACAT CATCCAGGCT 
    TTTGGGTTAC AGCCCAAAGA TGCCAATGGC AAGTGTGACC CCTATGTTAA GATCACGCTT 
    GGCAAGAAAT CAGTCGATGA CCACGACAAT TACATACCCT GTACTTTGGA GCCCGTCTTT 
    GGAAAGATGT TTGAGCTGAC CTGCTGTCTG CCTCTTGAGA AAGACCTGAA GATTACACTC 
    TATGATTACG ACATGGTGAC TAAAGATGAG AAAATCGGAG AGACCATCAT TGATTTGGAG 
    AACCGCTTCT TGTCTCGTCA TGGTGCTCGC TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA 
    GGAATTAACC AGTGGCGAGA TCAACTTAAA CCATCTCAGC TACTTCATCG TCTGTGTGAG 
    AGGCGAAACT ACAAACTCCC CGTCTACAAG CAGGATCGAA TTTACTTCAG AGGACATGAA 
    TACACTGTCG CAGACCTTGA TGAAGGGAAA CCTCTTAACC CTCACTTGGG ACCAGTGATG 
    GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG CATGGTTTGG TGCCTGAACA TGTCGAGACG 
    CGACCCCTCT ACAGCCCCCT TCAGCCAGAT ATAGAACAGG GGCGACTACA AATGTGGGTG 
    GACCTGTTCC CTAAGTCTTT AGGCCCTCCA GGTCCTCCAT TCAACATCAC CCCACGCAAA 
    GCTAAAAAGT TCTTCCTGCG CTGTATTATA TGGAACACAA GAGATGTAAT CTTGGATGAT 
    GTCAGCGTTA CCGGGGAGAA GATGAGTGAC ATTTATGTGA AAGGCTGGAT GCACGGCCAT 
    GAGGAGAACA AGCAGAAAAC AGACGTGCAC TACCGATCGC TGGGTGGTGA GGGTAACTTT 
    AACTGGCGCT TCCTCTTCCC CTTCCACTAT TTACCTGCGG AACAACTGTG CACCATTGAT 
    AAAAAGGAGC ACTTTTGGAG TCTGGATAAA GTTGAAACGA AGCTTCCTCC TAAACTTACT 
    ATCCAGATTT GGGACAATGA CAAGTTCTCC TTTGATGATT TCCTCGGCTC TTTGGAGATG 
    GATCTGAACC ACATGCTGAG TCCTGCGAAG TCTCCAGAGA AGTGTGGTCT TGAGATGCTC 
    ACTCAGTCCC AGGACAAACT GGTCTCTTTG TTTGAGCAGA AAACGGTGAA AGGCTGGTGG 
    CCGTGTGTCT GTGACAAAGA AGACAAGAAG ATACTTGCTG GAAAGGTGGA GATGTCTCTG 
    GAAATAGTCT GTGAACAGGA GCAGGATGAG AGGCCTGCAG GACTCGGCCG AGATGAGCCC 
    AACATGAATC CCCATCTAGA GGAGCCTCAG CGTCCCGAGA CATCATTCTT ATGGTTCTCG 
    TCCCCATATA AGACGTTTAG GTTCATTGTG TGGAGGAGGT TTAAGTGGCT TATTCTGGGC 
    CTCTTTATAC TCTTTTTCAT TATTCTCTTC CTTGGAGTAT TTCTCTACGC CTTCCCTAAC 
    TATGCTGCTA TGAAGATGGT TGGACCCTTC AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333235.1
    CDS307..6603
    Translation

    Target ORF information:

    RefSeq Version XM_021477560.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021477560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT 
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC 
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC 
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG 
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT 
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG 
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC 
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC 
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT 
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG 
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA 
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT 
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT 
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA 
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC 
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG 
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT 
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA 
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA 
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA 
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG 
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA 
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG 
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG 
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA 
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG 
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC 
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG 
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG 
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT 
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG 
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT 
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT 
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC 
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC 
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG 
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT 
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC 
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG 
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA 
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG 
    GATGAGCTTG TGCTTCCAGG GTTCCTCTCT GAGAATCAAC AGCAATGGCC AGAGGACTCT 
    GATTTGCCCT ATCCACCACC TCAGAGGGAG CCAAACGTCT ACATGGTGCC ACAGGGGATA 
    AAACCTGTCC TACAGCGCAC TGCTATTGAG ATACTGGCAT GGGGTTTGCG TAACCTGAAG 
    AGTTTCCAGC TGGCCAGTGT CACGTCTCCC AGTCTTCAGG TGGAATGTGG CGGATCTGTG 
    GTTCAGTCGT GCGTCATTCG AAATATTAAG AAGAACCCTA ATTTTGACGT CAACATCCTG 
    TTCATTGATG TCCGATTGCC TAAGGAAGAG CTCTACATGC CCCCATTGGT GATCAAGGTT 
    ATAGACAACC GGCAGTTTGG CAGGAAGCCT GTGGTGGGCC AGTGTTCAGT CCACTCGCTC 
    AATGAGTTCT ACTTCCAGCC GGAAAGTCTG GAGAGAGACT CAACAGAAGA GGAATTTGAA 
    GTTATTGCTC ACACACCTAG AGATGATGTT CTGGTTGATA TTGATGACAA GGAGCCTCTA 
    ATCCCTGGAC AGGATGAATT TATGGACTGG TGGAGCAAGT TTTATGCCTC CATGGGTGAG 
    AGGACCAAGT GTGGGAGTTA TCTGGAGAAA GGGTTCGATA TGCTGCAGGT GTATGACAGA 
    GAACTGGAGA AGACTGAAGG CTTTGAGCAA TTTGCTGATT TCTGTCAAAC GTTTAAACTT 
    TACCGTGGAA GAACCCAGAA TGACAGCGAA GATCCTTCCG TTGTTGGAGA GTTCAAGGGT 
    TCGTTTAAGA TCTATCCATT GCCTGATGAT CCCTCTATGC CTGTACCCAC ACGTCAGTTT 
    CGCAAACTCC CCCCGAACGG TATAGAGGAG TGCTTGGTAC GCATCTACAT CATCCAGGCT 
    TTTGGGTTAC AGCCCAAAGA TGCCAATGGC AAGTGTGACC CCTATGTTAA GATCACGCTT 
    GGCAAGAAAT CAGTCGATGA CCACGACAAT TACATACCCT GTACTTTGGA GCCCGTCTTT 
    GGAAAGATGT TTGAGCTGAC CTGCTGTCTG CCTCTTGAGA AAGACCTGAA GATTACACTC 
    TATGATTACG ACATGGTGAC TAAAGATGAG AAAATCGGAG AGACCATCAT TGATTTGGAG 
    AACCGCTTCT TGTCTCGTCA TGGTGCTCGC TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA 
    GGAATTAACC AGTGGCGAGA TCAACTTAAA CCATCTCAGC TACTTCATCG TCTGTGTGAG 
    AGGCGAAACT ACAAACTCCC CGTCTACAAG CAGGATCGAA TTTACTTCAG AGGACATGAA 
    TACACTGTCG CAGACCTTGA TGAAGGGAAA CCTCTTAACC CTCACTTGGG ACCAGTGATG 
    GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG CATGGTTTGG TGCCTGAACA TGTCGAGACG 
    CGACCCCTCT ACAGCCCCCT TCAGCCAGAT ATAGAACAGG GGCGACTACA AATGTGGGTG 
    GACCTGTTCC CTAAGTCTTT AGGCCCTCCA GGTCCTCCAT TCAACATCAC CCCACGCAAA 
    GCTAAAAAGT TCTTCCTGCG CTGTATTATA TGGAACACAA GAGATGTAAT CTTGGATGAT 
    GTCAGCGTTA CCGGGGAGAA GATGAGTGAC ATTTATGTGA AAGGCTGGAT GCACGGCCAT 
    GAGGAGAACA AGCAGAAAAC AGACGTGCAC TACCGATCGC TGGGTGGTGA GGGTAACTTT 
    AACTGGCGCT TCCTCTTCCC CTTCCACTAT TTACCTGCGG AACAACTGTG CACCATTGAT 
    AAAAAGGAGC ACTTTTGGAG TCTGGATAAA GTTGAAACGA AGCTTCCTCC TAAACTTACT 
    ATCCAGATTT GGGACAATGA CAAGTTCTCC TTTGATGATT TCCTCGGCTC TTTGGAGATG 
    GATCTGAACC ACATGCTGAG TCCTGCGAAG TCTCCAGAGA AGTGTGGTCT TGAGATGCTC 
    ACTCAGTCCC AGGACAAACT GGTCTCTTTG TTTGAGCAGA AAACGGTGAA AGGCTGGTGG 
    CCGTGTGTCT GTGACAAAGA AGACAAGAAG ATACTTGCTG GAAAGGTGGA GATGTCTCTG 
    GAAATAGTCT GTGAACAGGA GCAGGATGAG AGGCCTGCAG GACTCGGCCG AGATGAGCCC 
    AACATGAATC CCCATCTAGA GGAGCCTCAG CGTCCCGAGA CATCATTCTT ATGGTTCTCG 
    TCCCCATATA AGACGTTTAG GTTCATTGTG TGGAGGAGGT TTAAGTGGCT TATTCTGGGC 
    CTCTTTATAC TCTTTTTCAT TATTCTCTTC CTTGGAGTAT TTCTCTACGC CTTCCCTAAC 
    TATGCTGCTA TGAAGATGGT TGGACCCTTC AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    dysf ( XM_021477559.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333234.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa43390
    Clone ID Related Accession (Same CDS sequence) XM_021477559.1
    Accession Version XM_021477559.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT 
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC 
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC 
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG 
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT 
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG 
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC 
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC 
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT 
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG 
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA 
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT 
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT 
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA 
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC 
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG 
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT 
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA 
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA 
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA 
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG 
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA 
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG 
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG 
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA 
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG 
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC 
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG 
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG 
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT 
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG 
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT 
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT 
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC 
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC 
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG 
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT 
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC 
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG 
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA 
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG 
    GATGAGCTTG TGCTTCCAGG GTTCCTCTCT GAGAATCAAC AGCAATGGCC AGAGGACTCT 
    GATTTGCCCT ATCCACCACC TCAGAGGGAG CCAAACGTCT ACATGGTGCC ACAGGGGATA 
    AAACCTGTCC TACAGCGCAC TGCTATTGAG ATACTGGCAT GGGGTTTGCG TAACCTGAAG 
    AGTTTCCAGC TGGCCAGTGT CACGTCTCCC AGTCTTCAGG TGGAATGTGG CGGATCTGTG 
    GTTCAGTCGT GCGTCATTCG AAATATTAAG AAGAACCCTA ATTTTGACGT CAACATCCTG 
    TTCATTGATG TCCGATTGCC TAAGGAAGAG CTCTACATGC CCCCATTGGT GATCAAGGTT 
    ATAGACAACC GGCAGTTTGG CAGGAAGCCT GTGGTGGGCC AGTGTTCAGT CCACTCGCTC 
    AATGAGTTCT ACTTCCAGCC GGAAAGTCTG GAGAGAGACT CAACAGAAGA GGAATTTGAA 
    GTTATTGCTC ACACACCTAG AGATGATGTT CTGGTTGATA TTGATGACAA GGAGCCTCTA 
    ATCCCTGGAC AGCAGGATGA ATTTATGGAC TGGTGGAGCA AGTTTTATGC CTCCATGGGT 
    GAGAGGACCA AGTGTGGGAG TTATCTGGAG AAAGGGTTCG ATATGCTGCA GGTGTATGAC 
    AGAGAACTGG AGAAGACTGA AGGCTTTGAG CAATTTGCTG ATTTCTGTCA AACGTTTAAA 
    CTTTACCGTG GAAGAACCCA GAATGACAGC GAAGATCCTT CCGTTGTTGG AGAGTTCAAG 
    GGTTCGTTTA AGATCTATCC ATTGCCTGAT GATCCCTCTA TGCCTGTACC CACACGTCAG 
    TTTCGCAAAC TCCCCCCGAA CGGTATAGAG GAGTGCTTGG TACGCATCTA CATCATCCAG 
    GCTTTTGGGT TACAGCCCAA AGATGCCAAT GGCAAGTGTG ACCCCTATGT TAAGATCACG 
    CTTGGCAAGA AATCAGTCGA TGACCACGAC AATTACATAC CCTGTACTTT GGAGCCCGTC 
    TTTGGAAAGA TGTTTGAGCT GACCTGCTGT CTGCCTCTTG AGAAAGACCT GAAGATTACA 
    CTCTATGATT ACGACATGGT GACTAAAGAT GAGAAAATCG GAGAGACCAT CATTGATTTG 
    GAGAACCGCT TCTTGTCTCG TCATGGTGCT CGCTGCGGCC TTCCGCAGTC CTACTGTCTG 
    TCAGGAATTA ACCAGTGGCG AGATCAACTT AAACCATCTC AGCTACTTCA TCGTCTGTGT 
    GAGAGGCGAA ACTACAAACT CCCCGTCTAC AAGCAGGATC GAATTTACTT CAGAGGACAT 
    GAATACACTG TCGCAGACCT TGATGAAGGG AAACCTCTTA ACCCTCACTT GGGACCAGTG 
    ATGGAGCGTC TGGCTCTGTT GGTGTTGAGG AGGCATGGTT TGGTGCCTGA ACATGTCGAG 
    ACGCGACCCC TCTACAGCCC CCTTCAGCCA GATATAGAAC AGGGGCGACT ACAAATGTGG 
    GTGGACCTGT TCCCTAAGTC TTTAGGCCCT CCAGGTCCTC CATTCAACAT CACCCCACGC 
    AAAGCTAAAA AGTTCTTCCT GCGCTGTATT ATATGGAACA CAAGAGATGT AATCTTGGAT 
    GATGTCAGCG TTACCGGGGA GAAGATGAGT GACATTTATG TGAAAGGCTG GATGCACGGC 
    CATGAGGAGA ACAAGCAGAA AACAGACGTG CACTACCGAT CGCTGGGTGG TGAGGGTAAC 
    TTTAACTGGC GCTTCCTCTT CCCCTTCCAC TATTTACCTG CGGAACAACT GTGCACCATT 
    GATAAAAAGG AGCACTTTTG GAGTCTGGAT AAAGTTGAAA CGAAGCTTCC TCCTAAACTT 
    ACTATCCAGA TTTGGGACAA TGACAAGTTC TCCTTTGATG ATTTCCTCGG CTCTTTGGAG 
    ATGGATCTGA ACCACATGCT GAGTCCTGCG AAGTCTCCAG AGAAGTGTGG TCTTGAGATG 
    CTCACTCAGT CCCAGGACAA ACTGGTCTCT TTGTTTGAGC AGAAAACGGT GAAAGGCTGG 
    TGGCCGTGTG TCTGTGACAA AGAAGACAAG AAGATACTTG CTGGAAAGGT GGAGATGTCT 
    CTGGAAATAG TCTGTGAACA GGAGCAGGAT GAGAGGCCTG CAGGACTCGG CCGAGATGAG 
    CCCAACATGA ATCCCCATCT AGAGGAGCCT CAGCGTCCCG AGACATCATT CTTATGGTTC 
    TCGTCCCCAT ATAAGACGTT TAGGTTCATT GTGTGGAGGA GGTTTAAGTG GCTTATTCTG 
    GGCCTCTTTA TACTCTTTTT CATTATTCTC TTCCTTGGAG TATTTCTCTA CGCCTTCCCT 
    AACTATGCTG CTATGAAGAT GGTTGGACCC TTCAGTGGAC TAGGAAAGGG AACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333234.1
    CDS307..6606
    Translation

    Target ORF information:

    RefSeq Version XM_021477559.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021477559.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGAT 
    GAGTTTCCTG CCAGGACCCT CCACGACACT ATGGATGACA GTATAGGCTT TCTTCCGACC 
    TTTGGACCCT GCTTTGTCAA CATGTATGGG AGTCCTCGAG AGTTCACAGC TTTCTCAGAC 
    CCACACGAGG CACTAAACCT GGGAAAGGGA GAGGGTGTGG CCTATCGGGG ACGTGTGTTG 
    TTGGAGCTCA TAACAAAGCT TTCTGATCAG GTGGACCAAA GGTCTGAAGA CATCAACTCT 
    GATGACCTGC TAGTGGTAGA AAAATTCATG CGGAAGAGGA AGTTTTCCCT GTTTGCTGCG 
    TTCTACTCGG CCACTCTCCT TCAAGATGTG GACGATGCAG TTCAGTTTGA AGTCAGTATC 
    GGTAACTACG GCAACAAGTT CGACAACACC TGTTTGCCTC TGGCATCCAC CACTCAATTC 
    AGCAGAGCTG TGTTCGACGG CTGCCATTAC TATTACCTGC CATGGGGAAA TGTGAAACCT 
    GTGGTGGTGG TGTCTTCAGA GTGGGAAGAC ATTGGCCCTC GTATAGAGGC CCTCAACATG 
    CTGCTTAGCA TAGTCGACCG ACTGGAGTCA AACTTGGAAC AGGTACAGCT GGAGCTCAAA 
    GCAAGAAGTG ACGATCAGGA AGTCGAGCAG AGGGTTGTCC AGCTGCTTGA TGATGTCATT 
    GCAGACTGCA GTGAGGAACT CCCTGAGTTG GACAAATGGC CTTGTGTGAC TCCACTGGAT 
    AAAGCTCTGA GGAATCTGCG CAAGATTAAC ATGCAGCAGA TAGTGCAGGC GGCCCTGGCA 
    CTCAAACATG GACCAGAGAC TGAACTGAGT GCAGTGCTGG AGCAGGCTGA GGACTGGACC 
    ATGAGACTAC GCTACCTCTC TGAGGAGCCC CAAAACAGTC TGCCAGATAT AATAATCTGG 
    ATGCTTCAGG GAGACAGACG AGTGGCCTAC CACAGAATCC CTGCCCATCA AGTTCTCTTT 
    TCCTATGGTT ACTGTGGCAA GCACTGTGGC CAACTTCAGA CGGTTTTCAT GAAGTGTCCA 
    CAGGAAGATG GGGGATGTAG TAAAATCCCA GCTCAGCTCA GAATCAAGGT GTGGTTCGGA 
    CTGACTGCGG ATGTCAAATC TTTTAACCAG TTTGCAGAGG GCAAACTCTC AGTGTTTGCA 
    GAAACGTATG AGAACCAGAC TAAACTGGCT CTGGTGGGGA ACTGGGGTAC GACGGGTCTG 
    ACTTACCCCA AGTTCAGTGA TGTGACCGGC AGAGTCAAAC TGCCAAAGGA GAGCTTCAAA 
    CCCTCTTCAG GCTGGAGCTG GGCAGGAGAA TGGTTCATCA GCCCAGAGAA AACGATGCTG 
    TATGATGCTG ATGCAGGTCA TATAAGCTTT TTGGAGGAGG TGTTTGAAAA TCAGTTGCGG 
    TTGCCAGGAG GACAGTGGAT TGGCATGCCG GAGGGGTACA CAGATGTGAA TGGAGAAAAA 
    GCTGTTCCTA AAGATGAGAT CGAGTGTCCC CCTGGCTGGG TGTGGGAGGA TATTGAGTGG 
    AGTGAGGATC TGAAGCGTGC AGTGGATGAT CAAGGGTGGG AATATGGAAT AACCCTTCCC 
    CCGGACCGCC GACCAAAGTC CTGGGTTCCA TCTGAGAAGA TGTACCATAC CAACCGTCGG 
    AGACGCTGGA TCAGACTCCG CCGTAGAGAC ATGCAGAAAA TGGAGGCGCT CAGAAAACAG 
    CGGCCAGACG AGTCCGAGCG TGAGGGCTGG GAATATGCCT CTTTATTTGG CTGGAAGTTT 
    CACCTCAAGC AGAAGAAAAC CGACTTGTTC CGTCGCCGGC GCTGGAGACG TCGAATGGAG 
    CCATTAGAAA AGACGGGGCC GGCGGCCATC TTTGCCTTGG AGTGTTCGCT GAGCAGCTTT 
    ACTGATGAAA AGAATGACGA TAAGTCAGTC ACTACAACAT TTGGTGTCAG CCGGCCGACT 
    ATCTCCTGCT TCTTTGATAG TGGGACCCTG TATCATTTGC GCTGTTACTT GTATCAGGCC 
    AGAGACCTGC TGGCTATGGA CAAGGACAGT TTCTCGGATG CCTATGCCGT TGTATCGTTC 
    CTTCATCAGA GTCAGAAGAC GGTGACTGTT CGAAACACAC TCAACCCCAC CTGGGACCAG 
    ACCCTCATTT TCTACGAAGT GGAAATTTTT GGAGACCATC TTGTGACTGA GAGGAGCCCT 
    CCAAACATTG TGGTGGAGCT GTACGATCAG GACACCTATG GAGCTGATGA GTTCATGGGC 
    CGCTGTGTTT GCCAACCATC AATGGCATCT TCCCCTCGCC TCGCCTGGTA TCCCATCCAG 
    AAAGCAGGGA ATTCTGCAGG AGATCTGCTT GCTGCTTTTG AACTGATCCG CAGAGATAAA 
    CCTGCAGTTC ATCACATCCC GGGTCAAGAG GGAGATATTA TACCACCCTC ACATATCATG 
    GATGAGCTTG TGCTTCCAGG GTTCCTCTCT GAGAATCAAC AGCAATGGCC AGAGGACTCT 
    GATTTGCCCT ATCCACCACC TCAGAGGGAG CCAAACGTCT ACATGGTGCC ACAGGGGATA 
    AAACCTGTCC TACAGCGCAC TGCTATTGAG ATACTGGCAT GGGGTTTGCG TAACCTGAAG 
    AGTTTCCAGC TGGCCAGTGT CACGTCTCCC AGTCTTCAGG TGGAATGTGG CGGATCTGTG 
    GTTCAGTCGT GCGTCATTCG AAATATTAAG AAGAACCCTA ATTTTGACGT CAACATCCTG 
    TTCATTGATG TCCGATTGCC TAAGGAAGAG CTCTACATGC CCCCATTGGT GATCAAGGTT 
    ATAGACAACC GGCAGTTTGG CAGGAAGCCT GTGGTGGGCC AGTGTTCAGT CCACTCGCTC 
    AATGAGTTCT ACTTCCAGCC GGAAAGTCTG GAGAGAGACT CAACAGAAGA GGAATTTGAA 
    GTTATTGCTC ACACACCTAG AGATGATGTT CTGGTTGATA TTGATGACAA GGAGCCTCTA 
    ATCCCTGGAC AGCAGGATGA ATTTATGGAC TGGTGGAGCA AGTTTTATGC CTCCATGGGT 
    GAGAGGACCA AGTGTGGGAG TTATCTGGAG AAAGGGTTCG ATATGCTGCA GGTGTATGAC 
    AGAGAACTGG AGAAGACTGA AGGCTTTGAG CAATTTGCTG ATTTCTGTCA AACGTTTAAA 
    CTTTACCGTG GAAGAACCCA GAATGACAGC GAAGATCCTT CCGTTGTTGG AGAGTTCAAG 
    GGTTCGTTTA AGATCTATCC ATTGCCTGAT GATCCCTCTA TGCCTGTACC CACACGTCAG 
    TTTCGCAAAC TCCCCCCGAA CGGTATAGAG GAGTGCTTGG TACGCATCTA CATCATCCAG 
    GCTTTTGGGT TACAGCCCAA AGATGCCAAT GGCAAGTGTG ACCCCTATGT TAAGATCACG 
    CTTGGCAAGA AATCAGTCGA TGACCACGAC AATTACATAC CCTGTACTTT GGAGCCCGTC 
    TTTGGAAAGA TGTTTGAGCT GACCTGCTGT CTGCCTCTTG AGAAAGACCT GAAGATTACA 
    CTCTATGATT ACGACATGGT GACTAAAGAT GAGAAAATCG GAGAGACCAT CATTGATTTG 
    GAGAACCGCT TCTTGTCTCG TCATGGTGCT CGCTGCGGCC TTCCGCAGTC CTACTGTCTG 
    TCAGGAATTA ACCAGTGGCG AGATCAACTT AAACCATCTC AGCTACTTCA TCGTCTGTGT 
    GAGAGGCGAA ACTACAAACT CCCCGTCTAC AAGCAGGATC GAATTTACTT CAGAGGACAT 
    GAATACACTG TCGCAGACCT TGATGAAGGG AAACCTCTTA ACCCTCACTT GGGACCAGTG 
    ATGGAGCGTC TGGCTCTGTT GGTGTTGAGG AGGCATGGTT TGGTGCCTGA ACATGTCGAG 
    ACGCGACCCC TCTACAGCCC CCTTCAGCCA GATATAGAAC AGGGGCGACT ACAAATGTGG 
    GTGGACCTGT TCCCTAAGTC TTTAGGCCCT CCAGGTCCTC CATTCAACAT CACCCCACGC 
    AAAGCTAAAA AGTTCTTCCT GCGCTGTATT ATATGGAACA CAAGAGATGT AATCTTGGAT 
    GATGTCAGCG TTACCGGGGA GAAGATGAGT GACATTTATG TGAAAGGCTG GATGCACGGC 
    CATGAGGAGA ACAAGCAGAA AACAGACGTG CACTACCGAT CGCTGGGTGG TGAGGGTAAC 
    TTTAACTGGC GCTTCCTCTT CCCCTTCCAC TATTTACCTG CGGAACAACT GTGCACCATT 
    GATAAAAAGG AGCACTTTTG GAGTCTGGAT AAAGTTGAAA CGAAGCTTCC TCCTAAACTT 
    ACTATCCAGA TTTGGGACAA TGACAAGTTC TCCTTTGATG ATTTCCTCGG CTCTTTGGAG 
    ATGGATCTGA ACCACATGCT GAGTCCTGCG AAGTCTCCAG AGAAGTGTGG TCTTGAGATG 
    CTCACTCAGT CCCAGGACAA ACTGGTCTCT TTGTTTGAGC AGAAAACGGT GAAAGGCTGG 
    TGGCCGTGTG TCTGTGACAA AGAAGACAAG AAGATACTTG CTGGAAAGGT GGAGATGTCT 
    CTGGAAATAG TCTGTGAACA GGAGCAGGAT GAGAGGCCTG CAGGACTCGG CCGAGATGAG 
    CCCAACATGA ATCCCCATCT AGAGGAGCCT CAGCGTCCCG AGACATCATT CTTATGGTTC 
    TCGTCCCCAT ATAAGACGTT TAGGTTCATT GTGTGGAGGA GGTTTAAGTG GCTTATTCTG 
    GGCCTCTTTA TACTCTTTTT CATTATTCTC TTCCTTGGAG TATTTCTCTA CGCCTTCCCT 
    AACTATGCTG CTATGAAGAT GGTTGGACCC TTCAGTGGAC TAGGAAAGGG AACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    dysf ( XM_017356879.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212368.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa59639
    Clone ID Related Accession (Same CDS sequence) XM_017356879.1 , XM_017356879.2
    Accession Version XM_017356879.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product dysferlin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG 
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT 
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG 
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG 
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG 
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC 
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT 
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT 
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT 
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC 
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG 
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC 
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG 
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA 
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA 
    AACAGTCTGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC 
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA 
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT 
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT 
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG 
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA 
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG 
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG 
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG 
    GGGTACACAG ATGTGAATGG AGAAAAAGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT 
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGT GAGGATCTGA AGCGTGCAGT GGATGATCAA 
    GGGTGGGAAT ATGGAATAAC CCTTCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT 
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG 
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA 
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT 
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CGGGGCCGGC GGCCATCTTT 
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT 
    ACAACATTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT 
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC 
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTT CATCAGAGTC AGAAGACGGT GACTGTTCGA 
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA 
    GACCATCTTG TGACTGAGAG GAGCCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC 
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC 
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT 
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA 
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG 
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA 
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA 
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT 
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG 
    AACCCTAATT TTGACGTCAA CATCCTGTTC ATTGATGTCC GATTGCCTAA GGAAGAGCTC 
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG 
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG 
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG 
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGC AGGATGAATT TATGGACTGG 
    TGGAGCAAGT TTTATGCCTC CATGGGTGAG AGGACCAAGT GTGGGAGTTA TCTGGAGAAA 
    GGGTTCGATA TGCTGCAGGT GTATGACAGA GAACTGGAGA AGACTGAAGG CTTTGAGCAA 
    TTTGCTGATT TCTGTCAAAC GTTTAAACTT TACCGTGGAA GAACCCAGAA TGACAGCGAA 
    GATCCTTCCG TTGTTGGAGA GTTCAAGGGT TCGTTTAAGA TCTATCCATT GCCTGATGAT 
    CCCTCTATGC CTGTACCCAC ACGTCAGTTT CGCAAACTCC CCCCGAACGG TATAGAGGAG 
    TGCTTGGTAC GCATCTACAT CATCCAGGCT TTTGGGTTAC AGCCCAAAGA TGCCAATGGC 
    AAGTGTGACC CCTATGTTAA GATCACGCTT GGCAAGAAAT CAGTCGATGA CCACGACAAT 
    TACATACCCT GTACTTTGGA GCCCGTCTTT GGAAAGATGT TTGAGCTGAC CTGCTGTCTG 
    CCTCTTGAGA AAGACCTGAA GATTACACTC TATGATTACG ACATGGTGAC TAAAGATGAG 
    AAAATCGGAG AGACCATCAT TGATTTGGAG AACCGCTTCT TGTCTCGTCA TGGTGCTCGC 
    TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA GGAATTAACC AGTGGCGAGA TCAACTTAAA 
    CCATCTCAGC TACTTCATCG TCTGTGTGAG AGGCGAAACT ACAAACTCCC CGTCTACAAG 
    CAGGATCGAA TTTACTTCAG AGGACATGAA TACACTGTCG CAGACCTTGA TGAAGGGAAA 
    CCTCTTAACC CTCACTTGGG ACCAGTGATG GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG 
    CATGGTTTGG TGCCTGAACA TGTCGAGACG CGACCCCTCT ACAGCCCCCT TCAGCCAGAT 
    ATAGAACAGG GGCGACTACA AATGTGGGTG GACCTGTTCC CTAAGTCTTT AGGCCCTCCA 
    GGTCCTCCAT TCAACATCAC CCCACGCAAA GCTAAAAAGT TCTTCCTGCG CTGTATTATA 
    TGGAACACAA GAGATGTAAT CTTGGATGAT GTCAGCGTTA CCGGGGAGAA GATGAGTGAC 
    ATTTATGTGA AAGGCTGGAT GCACGGCCAT GAGGAGAACA AGCAGAAAAC AGACGTGCAC 
    TACCGATCGC TGGGTGGTGA GGGTAACTTT AACTGGCGCT TCCTCTTCCC CTTCCACTAT 
    TTACCTGCGG AACAACTGTG CACCATTGAT AAAAAGGAGC ACTTTTGGAG TCTGGATAAA 
    GTTGAAACGA AGCTTCCTCC TAAACTTACT ATCCAGATTT GGGACAATGA CAAGTTCTCC 
    TTTGATGATT TCCTCGGCTC TTTGGAGATG GATCTGAACC ACATGCTGAG TCCTGCGAAG 
    TCTCCAGAGA AGTGTGGTCT TGAGATGCTC ACTCAGTCCC AGGACAAACT GGTCTCTTTG 
    TTTGAGCAGA AAACGGTGAA AGGCTGGTGG CCGTGTGTCT GTGACAAAGA AGACAAGAAG 
    ATACTTGCTG GAAAGGTGGA GATGTCTCTG GAAATAGTCT GTGAACAGGA GCAGGATGAG 
    AGGCCTGCAG GACTCGGCCG AGATGAGCCC AACATGAATC CCCATCTAGA GGAGCCTCAG 
    CGTCCCGAGA CATCATTCTT ATGGTTCTCG TCCCCATATA AGACGTTTAG GTTCATTGTG 
    TGGAGGAGGT TTAAGTGGCT TATTCTGGGC CTCTTTATAC TCTTTTTCAT TATTCTCTTC 
    CTTGGAGTAT TTCTCTACGC CTTCCCTAAC TATGCTGCTA TGAAGATGGT TGGACCCTTC 
    AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212368.1
    CDS304..6570
    Translation

    Target ORF information:

    RefSeq Version XM_017356879.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017356879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG 
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT 
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG 
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG 
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG 
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC 
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT 
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT 
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT 
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC 
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG 
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC 
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG 
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA 
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA 
    AACAGTCTGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC 
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA 
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT 
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT 
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG 
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA 
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG 
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG 
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG 
    GGGTACACAG ATGTGAATGG AGAAAAAGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT 
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGT GAGGATCTGA AGCGTGCAGT GGATGATCAA 
    GGGTGGGAAT ATGGAATAAC CCTTCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT 
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG 
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA 
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT 
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CGGGGCCGGC GGCCATCTTT 
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT 
    ACAACATTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT 
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC 
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTT CATCAGAGTC AGAAGACGGT GACTGTTCGA 
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA 
    GACCATCTTG TGACTGAGAG GAGCCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC 
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC 
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT 
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA 
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG 
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA 
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA 
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT 
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG 
    AACCCTAATT TTGACGTCAA CATCCTGTTC ATTGATGTCC GATTGCCTAA GGAAGAGCTC 
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG 
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG 
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG 
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGC AGGATGAATT TATGGACTGG 
    TGGAGCAAGT TTTATGCCTC CATGGGTGAG AGGACCAAGT GTGGGAGTTA TCTGGAGAAA 
    GGGTTCGATA TGCTGCAGGT GTATGACAGA GAACTGGAGA AGACTGAAGG CTTTGAGCAA 
    TTTGCTGATT TCTGTCAAAC GTTTAAACTT TACCGTGGAA GAACCCAGAA TGACAGCGAA 
    GATCCTTCCG TTGTTGGAGA GTTCAAGGGT TCGTTTAAGA TCTATCCATT GCCTGATGAT 
    CCCTCTATGC CTGTACCCAC ACGTCAGTTT CGCAAACTCC CCCCGAACGG TATAGAGGAG 
    TGCTTGGTAC GCATCTACAT CATCCAGGCT TTTGGGTTAC AGCCCAAAGA TGCCAATGGC 
    AAGTGTGACC CCTATGTTAA GATCACGCTT GGCAAGAAAT CAGTCGATGA CCACGACAAT 
    TACATACCCT GTACTTTGGA GCCCGTCTTT GGAAAGATGT TTGAGCTGAC CTGCTGTCTG 
    CCTCTTGAGA AAGACCTGAA GATTACACTC TATGATTACG ACATGGTGAC TAAAGATGAG 
    AAAATCGGAG AGACCATCAT TGATTTGGAG AACCGCTTCT TGTCTCGTCA TGGTGCTCGC 
    TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA GGAATTAACC AGTGGCGAGA TCAACTTAAA 
    CCATCTCAGC TACTTCATCG TCTGTGTGAG AGGCGAAACT ACAAACTCCC CGTCTACAAG 
    CAGGATCGAA TTTACTTCAG AGGACATGAA TACACTGTCG CAGACCTTGA TGAAGGGAAA 
    CCTCTTAACC CTCACTTGGG ACCAGTGATG GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG 
    CATGGTTTGG TGCCTGAACA TGTCGAGACG CGACCCCTCT ACAGCCCCCT TCAGCCAGAT 
    ATAGAACAGG GGCGACTACA AATGTGGGTG GACCTGTTCC CTAAGTCTTT AGGCCCTCCA 
    GGTCCTCCAT TCAACATCAC CCCACGCAAA GCTAAAAAGT TCTTCCTGCG CTGTATTATA 
    TGGAACACAA GAGATGTAAT CTTGGATGAT GTCAGCGTTA CCGGGGAGAA GATGAGTGAC 
    ATTTATGTGA AAGGCTGGAT GCACGGCCAT GAGGAGAACA AGCAGAAAAC AGACGTGCAC 
    TACCGATCGC TGGGTGGTGA GGGTAACTTT AACTGGCGCT TCCTCTTCCC CTTCCACTAT 
    TTACCTGCGG AACAACTGTG CACCATTGAT AAAAAGGAGC ACTTTTGGAG TCTGGATAAA 
    GTTGAAACGA AGCTTCCTCC TAAACTTACT ATCCAGATTT GGGACAATGA CAAGTTCTCC 
    TTTGATGATT TCCTCGGCTC TTTGGAGATG GATCTGAACC ACATGCTGAG TCCTGCGAAG 
    TCTCCAGAGA AGTGTGGTCT TGAGATGCTC ACTCAGTCCC AGGACAAACT GGTCTCTTTG 
    TTTGAGCAGA AAACGGTGAA AGGCTGGTGG CCGTGTGTCT GTGACAAAGA AGACAAGAAG 
    ATACTTGCTG GAAAGGTGGA GATGTCTCTG GAAATAGTCT GTGAACAGGA GCAGGATGAG 
    AGGCCTGCAG GACTCGGCCG AGATGAGCCC AACATGAATC CCCATCTAGA GGAGCCTCAG 
    CGTCCCGAGA CATCATTCTT ATGGTTCTCG TCCCCATATA AGACGTTTAG GTTCATTGTG 
    TGGAGGAGGT TTAAGTGGCT TATTCTGGGC CTCTTTATAC TCTTTTTCAT TATTCTCTTC 
    CTTGGAGTAT TTCTCTACGC CTTCCCTAAC TATGCTGCTA TGAAGATGGT TGGACCCTTC 
    AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    dysf ( XM_017356879.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212368.1 Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID ODa59639
    Clone ID Related Accession (Same CDS sequence) XM_017356879.1 , XM_017356879.2
    Accession Version XM_017356879.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product dysferlin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356879.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG 
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT 
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG 
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG 
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG 
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC 
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT 
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT 
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT 
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC 
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG 
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC 
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG 
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA 
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA 
    AACAGTCTGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC 
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA 
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT 
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT 
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG 
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA 
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG 
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG 
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG 
    GGGTACACAG ATGTGAATGG AGAAAAAGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT 
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGT GAGGATCTGA AGCGTGCAGT GGATGATCAA 
    GGGTGGGAAT ATGGAATAAC CCTTCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT 
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG 
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA 
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT 
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CGGGGCCGGC GGCCATCTTT 
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT 
    ACAACATTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT 
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC 
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTT CATCAGAGTC AGAAGACGGT GACTGTTCGA 
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA 
    GACCATCTTG TGACTGAGAG GAGCCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC 
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC 
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT 
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA 
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG 
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA 
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA 
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT 
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG 
    AACCCTAATT TTGACGTCAA CATCCTGTTC ATTGATGTCC GATTGCCTAA GGAAGAGCTC 
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG 
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG 
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG 
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGC AGGATGAATT TATGGACTGG 
    TGGAGCAAGT TTTATGCCTC CATGGGTGAG AGGACCAAGT GTGGGAGTTA TCTGGAGAAA 
    GGGTTCGATA TGCTGCAGGT GTATGACAGA GAACTGGAGA AGACTGAAGG CTTTGAGCAA 
    TTTGCTGATT TCTGTCAAAC GTTTAAACTT TACCGTGGAA GAACCCAGAA TGACAGCGAA 
    GATCCTTCCG TTGTTGGAGA GTTCAAGGGT TCGTTTAAGA TCTATCCATT GCCTGATGAT 
    CCCTCTATGC CTGTACCCAC ACGTCAGTTT CGCAAACTCC CCCCGAACGG TATAGAGGAG 
    TGCTTGGTAC GCATCTACAT CATCCAGGCT TTTGGGTTAC AGCCCAAAGA TGCCAATGGC 
    AAGTGTGACC CCTATGTTAA GATCACGCTT GGCAAGAAAT CAGTCGATGA CCACGACAAT 
    TACATACCCT GTACTTTGGA GCCCGTCTTT GGAAAGATGT TTGAGCTGAC CTGCTGTCTG 
    CCTCTTGAGA AAGACCTGAA GATTACACTC TATGATTACG ACATGGTGAC TAAAGATGAG 
    AAAATCGGAG AGACCATCAT TGATTTGGAG AACCGCTTCT TGTCTCGTCA TGGTGCTCGC 
    TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA GGAATTAACC AGTGGCGAGA TCAACTTAAA 
    CCATCTCAGC TACTTCATCG TCTGTGTGAG AGGCGAAACT ACAAACTCCC CGTCTACAAG 
    CAGGATCGAA TTTACTTCAG AGGACATGAA TACACTGTCG CAGACCTTGA TGAAGGGAAA 
    CCTCTTAACC CTCACTTGGG ACCAGTGATG GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG 
    CATGGTTTGG TGCCTGAACA TGTCGAGACG CGACCCCTCT ACAGCCCCCT TCAGCCAGAT 
    ATAGAACAGG GGCGACTACA AATGTGGGTG GACCTGTTCC CTAAGTCTTT AGGCCCTCCA 
    GGTCCTCCAT TCAACATCAC CCCACGCAAA GCTAAAAAGT TCTTCCTGCG CTGTATTATA 
    TGGAACACAA GAGATGTAAT CTTGGATGAT GTCAGCGTTA CCGGGGAGAA GATGAGTGAC 
    ATTTATGTGA AAGGCTGGAT GCACGGCCAT GAGGAGAACA AGCAGAAAAC AGACGTGCAC 
    TACCGATCGC TGGGTGGTGA GGGTAACTTT AACTGGCGCT TCCTCTTCCC CTTCCACTAT 
    TTACCTGCGG AACAACTGTG CACCATTGAT AAAAAGGAGC ACTTTTGGAG TCTGGATAAA 
    GTTGAAACGA AGCTTCCTCC TAAACTTACT ATCCAGATTT GGGACAATGA CAAGTTCTCC 
    TTTGATGATT TCCTCGGCTC TTTGGAGATG GATCTGAACC ACATGCTGAG TCCTGCGAAG 
    TCTCCAGAGA AGTGTGGTCT TGAGATGCTC ACTCAGTCCC AGGACAAACT GGTCTCTTTG 
    TTTGAGCAGA AAACGGTGAA AGGCTGGTGG CCGTGTGTCT GTGACAAAGA AGACAAGAAG 
    ATACTTGCTG GAAAGGTGGA GATGTCTCTG GAAATAGTCT GTGAACAGGA GCAGGATGAG 
    AGGCCTGCAG GACTCGGCCG AGATGAGCCC AACATGAATC CCCATCTAGA GGAGCCTCAG 
    CGTCCCGAGA CATCATTCTT ATGGTTCTCG TCCCCATATA AGACGTTTAG GTTCATTGTG 
    TGGAGGAGGT TTAAGTGGCT TATTCTGGGC CTCTTTATAC TCTTTTTCAT TATTCTCTTC 
    CTTGGAGTAT TTCTCTACGC CTTCCCTAAC TATGCTGCTA TGAAGATGGT TGGACCCTTC 
    AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212368.1
    CDS307..6573
    Translation

    Target ORF information:

    RefSeq Version XM_017356879.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) (dysf), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017356879.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTTGAGGT GTTTGCTACA GAGAGCGACC AACCTGAGAA ATGTAGAGAA GGGGGAAAGG 
    CATAGCGACC CGCTTGCTAG CATCACTTTC AGAGGGACAA AAAAGAAGAC TAAAGTCATC 
    AAAAATAATG TCAATCCTGT CTGGAATGAG GGCTTTGAGT GGGACCTGAA GGGTGTTCCT 
    TTAGATTCGG GAGCAGAGCT TCATGTTGTC GTTAAAGACC ATGAGAAAAT GGGCAGGAAT 
    AGGTTTCTGG GGGAGTGTCG AGTTGCTCTT CGAGATGTGC TCAATTCTCC AAACCTGGCT 
    GCTACTTTTA CCGTGTCCCT GGTCGACACA AAGAGAAACA GCACTGGGGC CACAATAACC 
    CTGCAGGTCT CCTACATCCC TCCTCCAGGG ATGACGCCCA TCTTTCAGCC TCCTCCACAG 
    CCAGAGGCCC AGCACACACC GGTGGAGCTG GACACAGTCA CAGTTTTCTC ATTGGACACT 
    ATGGGCGAGG ATGACACTGA GAGCATGCTG ATGATGGAGG TGGCGGAGGA GCCTGAACCT 
    GTTCCAGGAC CCCAGGGGCA GGACATCGGC GCCCCTACAG CACCACCCAA AAAGGCTCCA 
    CCCAACTTTA ACCAGGGACT AAAGAAAAAG AAACGCCAAA GCAACAAATC CCCCCTGTCC 
    GACAAAGCCC AGGACCTCCA GGTTCGGCTG CGCATCATTG AAGGAAGGCA GCTTCCTGGT 
    GTCAACATCA AACCAGTCGT CAAAGTCACA GTTGGAGGAC AGACGAAGAG AACCCGCATT 
    AAAAAGGGCA ATAATCCATT TTTTGATGAG ACCTTTTTTC TAAACTTCTT TGAGAAACCT 
    TCAGATCTTT TTGATGAGCC AATATTCATA ACCGTATTTG ATTCCAAGTC ACTACGGAAA 
    GACTCTGTCA TTGGAGAATT CAAGCTGGAT GTTGGCATGG TTTATAATGA ACACCGACAT 
    GCTATTCTCA GAAAATGGTT GTTGCTGGCA GACCCTGATG ATCTTTCTGC AGGGGCCAGA 
    GGTTATTTGA AGGTCAGCAT GTTTGTGCTG GCCGCAGGAG ATGAACCTCC GATGGATAAG 
    AAAGAGGGTG TGGAGGAAAA GGAGGACATA GAGGCCAATC TGTTGAGACC AGCTGGTCTA 
    ACCCTGAGAG GAGCCACGTT CACTCTGAGA GTCTACAGAG CAGAAGACCT TCCTCAGATG 
    GATGATGCTC TTATTGACGA TGTCAAACAT TTCCTTGGCT TTGACACTAA TCGAAAGAAC 
    CTGGTGGATC CATTGGTGGA GGTCAACTTT GCAGGAAAAA CGATAAGTAG TAAGATTCTG 
    GAGAAATGCG CTAACCCTCA GTGGAACCAG TGCCTCACTC TGCCCATCAG GTTTCCATCC 
    ATGTGTGAAA AGATGAGAAT ACGTATTTTA GATTGGGATC GTGCGAGTCA TAATGATGTC 
    ATTGGCACTG CACACTTATG CATGTCTAAA ATCTCAGCCC CTGGAGGAGA TATTGAAGTG 
    GATGACAGTA TAGGCTTTCT TCCGACCTTT GGACCCTGCT TTGTCAACAT GTATGGGAGT 
    CCTCGAGAGT TCACAGCTTT CTCAGACCCA CACGAGGCAC TAAACCTGGG AAAGGGAGAG 
    GGTGTGGCCT ATCGGGGACG TGTGTTGTTG GAGCTCATAA CAAAGCTTTC TGATCAGGTG 
    GACCAAAGGT CTGAAGACAT CAACTCTGAT GACCTGCTAG TGGTAGAAAA ATTCATGCGG 
    AAGAGGAAGT TTTCCCTGTT TGCTGCGTTC TACTCGGCCA CTCTCCTTCA AGATGTGGAC 
    GATGCAGTTC AGTTTGAAGT CAGTATCGGT AACTACGGCA ACAAGTTCGA CAACACCTGT 
    TTGCCTCTGG CATCCACCAC TCAATTCAGC AGAGCTGTGT TCGACGGCTG CCATTACTAT 
    TACCTGCCAT GGGGAAATGT GAAACCTGTG GTGGTGGTGT CTTCAGAGTG GGAAGACATT 
    GGCCCTCGTA TAGAGGCCCT CAACATGCTG CTTAGCATAG TCGACCGACT GGAGTCAAAC 
    TTGGAACAGG TACAGCTGGA GCTCAAAGCA AGAAGTGACG ATCAGGAAGT CGAGCAGAGG 
    GTTGTCCAGC TGCTTGATGA TGTCATTGCA GACTGCAGTG AGGAACTCCC TGAGTTGGAC 
    AAATGGCCTT GTGTGACTCC ACTGGATAAA GCTCTGAGGA ATCTGCGCAA GATTAACATG 
    CAGCAGATAG TGCAGGCGGC CCTGGCACTC AAACATGGAC CAGAGACTGA ACTGAGTGCA 
    GTGCTGGAGC AGGCTGAGGA CTGGACCATG AGACTACGCT ACCTCTCTGA GGAGCCCCAA 
    AACAGTCTGC CAGATATAAT AATCTGGATG CTTCAGGGAG ACAGACGAGT GGCCTACCAC 
    AGAATCCCTG CCCATCAAGT TCTCTTTTCC TATGGTTACT GTGGCAAGCA CTGTGGCCAA 
    CTTCAGACGG TTTTCATGAA GTGTCCACAG GAAGATGGGG GATGTAGTAA AATCCCAGCT 
    CAGCTCAGAA TCAAGGTGTG GTTCGGACTG ACTGCGGATG TCAAATCTTT TAACCAGTTT 
    GCAGAGGGCA AACTCTCAGT GTTTGCAGAA ACGTATGAGA ACCAGACTAA ACTGGCTCTG 
    GTGGGGAACT GGGGTACGAC GGGTCTGACT TACCCCAAGT TCAGTGATGT GACCGGCAGA 
    GTCAAACTGC CAAAGGAGAG CTTCAAACCC TCTTCAGGCT GGAGCTGGGC AGGAGAATGG 
    TTCATCAGCC CAGAGAAAAC GATGCTGTAT GATGCTGATG CAGGTCATAT AAGCTTTTTG 
    GAGGAGGTGT TTGAAAATCA GTTGCGGTTG CCAGGAGGAC AGTGGATTGG CATGCCGGAG 
    GGGTACACAG ATGTGAATGG AGAAAAAGCT GTTCCTAAAG ATGAGATCGA GTGTCCCCCT 
    GGCTGGGTGT GGGAGGATAT TGAGTGGAGT GAGGATCTGA AGCGTGCAGT GGATGATCAA 
    GGGTGGGAAT ATGGAATAAC CCTTCCCCCG GACCGCCGAC CAAAGTCCTG GGTTCCATCT 
    GAGAAGATGT ACCATACCAA CCGTCGGAGA CGCTGGATCA GACTCCGCCG TAGAGACATG 
    CAGAAAATGG AGGCGCTCAG AAAACAGCGG CCAGACGAGT CCGAGCGTGA GGGCTGGGAA 
    TATGCCTCTT TATTTGGCTG GAAGTTTCAC CTCAAGCAGA AGAAAACCGA CTTGTTCCGT 
    CGCCGGCGCT GGAGACGTCG AATGGAGCCA TTAGAAAAGA CGGGGCCGGC GGCCATCTTT 
    GCCTTGGAGT GTTCGCTGAG CAGCTTTACT GATGAAAAGA ATGACGATAA GTCAGTCACT 
    ACAACATTTG GTGTCAGCCG GCCGACTATC TCCTGCTTCT TTGATAGTGG GACCCTGTAT 
    CATTTGCGCT GTTACTTGTA TCAGGCCAGA GACCTGCTGG CTATGGACAA GGACAGTTTC 
    TCGGATGCCT ATGCCGTTGT ATCGTTCCTT CATCAGAGTC AGAAGACGGT GACTGTTCGA 
    AACACACTCA ACCCCACCTG GGACCAGACC CTCATTTTCT ACGAAGTGGA AATTTTTGGA 
    GACCATCTTG TGACTGAGAG GAGCCCTCCA AACATTGTGG TGGAGCTGTA CGATCAGGAC 
    ACCTATGGAG CTGATGAGTT CATGGGCCGC TGTGTTTGCC AACCATCAAT GGCATCTTCC 
    CCTCGCCTCG CCTGGTATCC CATCCAGAAA GCAGGGAATT CTGCAGGAGA TCTGCTTGCT 
    GCTTTTGAAC TGATCCGCAG AGATAAACCT GCAGTTCATC ACATCCCGGG TCAAGAGGGA 
    GATATTATAC CACCCTCACA TATCATGGAT GAGCTTGTGC TTCCAGGGTT CCTCTCTGAG 
    AATCAACAGC AATGGCCAGA GGACTCTGAT TTGCCCTATC CACCACCTCA GAGGGAGCCA 
    AACGTCTACA TGGTGCCACA GGGGATAAAA CCTGTCCTAC AGCGCACTGC TATTGAGATA 
    CTGGCATGGG GTTTGCGTAA CCTGAAGAGT TTCCAGCTGG CCAGTGTCAC GTCTCCCAGT 
    CTTCAGGTGG AATGTGGCGG ATCTGTGGTT CAGTCGTGCG TCATTCGAAA TATTAAGAAG 
    AACCCTAATT TTGACGTCAA CATCCTGTTC ATTGATGTCC GATTGCCTAA GGAAGAGCTC 
    TACATGCCCC CATTGGTGAT CAAGGTTATA GACAACCGGC AGTTTGGCAG GAAGCCTGTG 
    GTGGGCCAGT GTTCAGTCCA CTCGCTCAAT GAGTTCTACT TCCAGCCGGA AAGTCTGGAG 
    AGAGACTCAA CAGAAGAGGA ATTTGAAGTT ATTGCTCACA CACCTAGAGA TGATGTTCTG 
    GTTGATATTG ATGACAAGGA GCCTCTAATC CCTGGACAGC AGGATGAATT TATGGACTGG 
    TGGAGCAAGT TTTATGCCTC CATGGGTGAG AGGACCAAGT GTGGGAGTTA TCTGGAGAAA 
    GGGTTCGATA TGCTGCAGGT GTATGACAGA GAACTGGAGA AGACTGAAGG CTTTGAGCAA 
    TTTGCTGATT TCTGTCAAAC GTTTAAACTT TACCGTGGAA GAACCCAGAA TGACAGCGAA 
    GATCCTTCCG TTGTTGGAGA GTTCAAGGGT TCGTTTAAGA TCTATCCATT GCCTGATGAT 
    CCCTCTATGC CTGTACCCAC ACGTCAGTTT CGCAAACTCC CCCCGAACGG TATAGAGGAG 
    TGCTTGGTAC GCATCTACAT CATCCAGGCT TTTGGGTTAC AGCCCAAAGA TGCCAATGGC 
    AAGTGTGACC CCTATGTTAA GATCACGCTT GGCAAGAAAT CAGTCGATGA CCACGACAAT 
    TACATACCCT GTACTTTGGA GCCCGTCTTT GGAAAGATGT TTGAGCTGAC CTGCTGTCTG 
    CCTCTTGAGA AAGACCTGAA GATTACACTC TATGATTACG ACATGGTGAC TAAAGATGAG 
    AAAATCGGAG AGACCATCAT TGATTTGGAG AACCGCTTCT TGTCTCGTCA TGGTGCTCGC 
    TGCGGCCTTC CGCAGTCCTA CTGTCTGTCA GGAATTAACC AGTGGCGAGA TCAACTTAAA 
    CCATCTCAGC TACTTCATCG TCTGTGTGAG AGGCGAAACT ACAAACTCCC CGTCTACAAG 
    CAGGATCGAA TTTACTTCAG AGGACATGAA TACACTGTCG CAGACCTTGA TGAAGGGAAA 
    CCTCTTAACC CTCACTTGGG ACCAGTGATG GAGCGTCTGG CTCTGTTGGT GTTGAGGAGG 
    CATGGTTTGG TGCCTGAACA TGTCGAGACG CGACCCCTCT ACAGCCCCCT TCAGCCAGAT 
    ATAGAACAGG GGCGACTACA AATGTGGGTG GACCTGTTCC CTAAGTCTTT AGGCCCTCCA 
    GGTCCTCCAT TCAACATCAC CCCACGCAAA GCTAAAAAGT TCTTCCTGCG CTGTATTATA 
    TGGAACACAA GAGATGTAAT CTTGGATGAT GTCAGCGTTA CCGGGGAGAA GATGAGTGAC 
    ATTTATGTGA AAGGCTGGAT GCACGGCCAT GAGGAGAACA AGCAGAAAAC AGACGTGCAC 
    TACCGATCGC TGGGTGGTGA GGGTAACTTT AACTGGCGCT TCCTCTTCCC CTTCCACTAT 
    TTACCTGCGG AACAACTGTG CACCATTGAT AAAAAGGAGC ACTTTTGGAG TCTGGATAAA 
    GTTGAAACGA AGCTTCCTCC TAAACTTACT ATCCAGATTT GGGACAATGA CAAGTTCTCC 
    TTTGATGATT TCCTCGGCTC TTTGGAGATG GATCTGAACC ACATGCTGAG TCCTGCGAAG 
    TCTCCAGAGA AGTGTGGTCT TGAGATGCTC ACTCAGTCCC AGGACAAACT GGTCTCTTTG 
    TTTGAGCAGA AAACGGTGAA AGGCTGGTGG CCGTGTGTCT GTGACAAAGA AGACAAGAAG 
    ATACTTGCTG GAAAGGTGGA GATGTCTCTG GAAATAGTCT GTGAACAGGA GCAGGATGAG 
    AGGCCTGCAG GACTCGGCCG AGATGAGCCC AACATGAATC CCCATCTAGA GGAGCCTCAG 
    CGTCCCGAGA CATCATTCTT ATGGTTCTCG TCCCCATATA AGACGTTTAG GTTCATTGTG 
    TGGAGGAGGT TTAAGTGGCT TATTCTGGGC CTCTTTATAC TCTTTTTCAT TATTCTCTTC 
    CTTGGAGTAT TTCTCTACGC CTTCCCTAAC TATGCTGCTA TGAAGATGGT TGGACCCTTC 
    AGTGGACTAG GAAAGGGAAC ACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission