NPHP4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPHP4
Entrez Gene ID	560234
Full Name	nephronophthisis 4
Synonyms	si:ch211-55k22.1,si:ch211-55k22.2,si:dkey-202c5.1
General protein information	Preferred Names nephronophthisis 4 Names nephrocystin-4 nephronophthisis 4
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005166952.3	XP_005167009.2	nephrocystin-4 isoform X2
XM_021478419.1	XP_021334094.1	nephrocystin-4 isoform X1
XM_017357450.1	XP_017212939.1	nephrocystin-4 isoform X3
XM_021472054.1	XP_021327729.1	nephrocystin-4 isoform X2
XM_017357449.1	XP_017212938.1	nephrocystin-4 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NPHP4	XP_005217234.1
Caenorhabditis elegans (roundworm)	nphp-4	NP_505982.2
Pan troglodytes (chimpanzee)	NPHP4	XP_003307840.2
Macaca mulatta (Rhesus monkey)	NPHP4	XP_002802192.1
Homo sapiens (human)	NPHP4	NP_055917.1
Canis lupus familiaris (dog)	NPHP4	NP_001129260.1
Mus musculus (house mouse)	Nphp4	NP_700473.2
Danio rerio (zebrafish)	nphp4	XP_005167009.1
Rattus norvegicus (Norway rat)	Nphp4	NP_001032739.2
Gallus gallus (chicken)	NPHP4	XP_003642568.2

Gene Ontology

The following NPHP4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPHP4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa143790	XM_021478419.1 Latest version!	Danio rerio nephronophthisis 4 (nphp4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa144980	XM_021472054.1 Latest version!	Danio rerio nephronophthisis 4 (nphp4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa43251	XM_005166952.3 Latest version!	Danio rerio nephronophthisis 4 (nphp4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa60047	XM_017357449.1 Latest version!	Danio rerio nephronophthisis 4 (nphp4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa60048	XM_017357450.1 Latest version!	Danio rerio nephronophthisis 4 (nphp4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPHP4 ( XM_021478419.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021334094.1 Danio rerio nephronophthisis 4 (nphp4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143790
Clone ID Related Accession (Same CDS sequence)	XM_021478419.1
Accession Version	XM_021478419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	nephrocystin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCACA CGACGGGGTC TCCATGGCAA CAGCAGCTCC CCTCTCTGCT CTCTCCCTCC 
CCGATGGCAT CGGCCCACCA GCTGTCACAT GTCGAGCGTC CAGTTGTTGG CAGTATTGCT 
CATCTGGAGC TGAGCGTGCA GGAGAGAGTG GGAGACAGAA GTGAGGAAGA GGATGAGATG 
CAGGAGTTGA CCTTCACCCC GGTGCACGCG CCTGTCATCA CCCTCGGGAC AAACGCTGCT 
GGCTCCTCCA GATCGACCTC CCGGAGATCT TTGGCGCAGC TCTTCTCTGC TGGTTTTCCT 
CAGATCCTGG ACCATCAGAA CCAGGTGGCC GAGGTTCTGG ATCCTTCTGA GCCTGTGAAC 
TTCAACCCCC AGAGAGAAGA AGCAGATTCT CTGCAGACAA ACACACTCAT CCTGCAGTTC 
CTGGCCTTTA CCAGGGTCCC TCAATCTGGT GTGAATTCAG ACTGGCCGAG CTCTGTCCAT 
TTCACCTTCC AGCTTTACCG CTTTCCTCCT GTCACCACCC AGCGGCTGAA GCTGCTTGAC 
CAGGAGAAGA CCAGCAAGTT CAAAGGCAGC TCTGATTCCC CTTGTGTGCT TTCTCTGCTC 
AACAAAGACG GCACACTTAG CTCTGGTTTT CCTGGTCTGC AGCTGCAGTA TCGAGTTGAC 
CCACAATTCC TAAAGCCAGG TGAACGGGGA TGGTTTCTGC GATACCTGGC ACTCCACTCA 
CTGCAGATTG ATGTCTGGGA CAGTGATTCC TTAATGCTGA TTGGCTCAGC TGCTGTGGAG 
CTGAAGCATA TGCTACGTCA AGGCCGTCCG GCTGTCCAGG TGAGCCATGA GCTAGAAGTG 
ATCACTACCG AGTACATGCA GGATGCCAGC TTTACTAGCA GACACGACAG CAAACACAGC 
GCCACTTCAG CCATCAGTGT CTACACTACT GTGAAAGGGA TTTTGCATCT ACGGCTCGGC 
AACATTGGTG GCAGTGTGTG GAATACTCAG AGTTCTCCAG CTGTTCCTCC GTCGTTCTCT 
CATGTTCTCC AGCCCAACGA TGGCCCTCAG GGTTTAGCAG AAGGAAGAGT GTCTGCTTTA 
ACACTCCTCC AGCTTAACGC GAGCAATGCC AGACGAGCCC GTCGGTTGCC AGAAATCAAC 
AGTGAACTGG CCAGACTCTT GCAAAGCAGC ATGAGGGAAG TGGGCGGAGC CGAAGAGAAG 
GAAGTGCGCC AGAGGAAGAT TGACAGAATG GCAGCTGTCC GTCAACACGA GGAGCAGAAA 
GACGAGCCCC CTTTTCAGGA CTACACAAAC GTCACGAGTC ACCGGGCGGA TCGAGTGCAG 
CATTTGCGAG ACCTGCGTGT GATTGAGGCC TATCGTGAGC GCTGTAAAAC ACAGGAGATC 
ACCAACATGC TCTCTCAGGC CATCACCACC AAACACACAC TCTACGCCAC CCTTGGTACG 
GCAGAGTTCT TCGAATTTGT GCTCAAGAAC CCATTCAATG TTCCTCAGAC AGTCACCATT 
GAATGTGATG ACCCAGAGCT CAGTGTACTG GTGAAGAGCG AAGAGTGGAG ATATTTTAAG 
GAGTTGACGA AGACTCTGAC TCCTCTAGAG GAGGAGATGT TTCACCTGAA GGAGAAAACA 
CTGACTCCTC AAGTTTACCT GCGGCCAAAA GAAAGCGTTC ACGTTCCCTT CAAGTACCAG 
ACCTTCGTTT GTGGTCACAG CTTTCCTCTT CAGGACTCAA AAAGCTTAAA GATTTCAAGA 
AGTCCACAGA TGGCTCAGAA ACATCTGTCC AATACAATCC AAGCCAAATC CATCAAGGTG 
TTGTTTCGTG GTGAGGACAG AAAGCCTCTG GCGATCTGCC AGGTGGATGT TGAACCAACG 
CCACATGTGG TGGACCAGAC CTTCCGCTTC TACCAGCCTG AGCTCACCTT CTTAAAGAAG 
GCCATTCGCC TGCCTGCAGC CTGGGACGAC ACAGCAGATG GATCTGCCAG CCTCCATGTG 
CACTGCAGTG ACCCCAATGT CATCTGCCAC ACCACCAGCC TGACTCCAGG AGAACCACAG 
GATGTTTACC TGAAAGTACC TGGAAGCCCA AGTCCACAGA TTAGAAAGTT CTTCATCACC 
ATTTTTACTG ACCCCTGGCT GGCCTCCCCT GCGCAGATCT GGCAGGTTTA CGTGCATTAC 
TTGCAAAGGA TGGACCTGAG CTGTGTGTGT GGTGCTCTGA GCTGGCATTC GCTGCTTCTG 
CGTGGCACAC AAGCCATTCG CAGGGTCAAG TGCTACACGT CGCATCCCCT CGAGCTTCAG 
GTGGATCCAG CTGAAGTTTT CGCTCTGCCG GCTCAGGGAG TTCAGGATGT TCGTGTGGGC 
CTGAGGGCTC TGAGGGCTGG AAGTCGCTTC TTTTACCTCA ATGTTGTTGA TGTGGACACA 
CGGCAGCTCG TGGACTCCTG GCTGCTGTGC ATCACATGCC GACAGCCTGT AATCTCCAAG 
GCGTTCGAAA TCAGCGTTCC TGTAGAAGGA GGGAAGGGCA GCAATAAAAA AATCAGTTTC 
ACAAACCCCT ACTCGTCCTC CAGAGTCTTC AATCTGCGCA CCGACCGCCC GGACCTGCTA 
CACTTTAAAG AGGAGCAGTT TCAGATTGGA GGAGGAGAGC TCTACACCAT TGGCCTGCGC 
TTTGCTCCAA GTCAGAACCC CGGCATGGAA GAGATTCTCA TCTTCATAAA TGACAAACAG 
GACAAGAATG AGGACACTTA CTGTGTTCGA GTCCAGTACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334094.1
CDS	95..2839
Translation	MSHTTGSPWQQQLPSLLSPSPMASAHQLSHVERPVVGSIAHLELSVQERVGDRSEEEDEMQELTFTPVHAPVITLGTNAAGSSRSTSRRSLAQLFSAGFPQILDHQNQVAEVLDPSEPVNFNPQREEADSLQTNTLILQFLAFTRVPQSGVNSDWPSSVHFTFQLYRFPPVTTQRLKLLDQEKTSKFKGSSDSPCVLSLLNKDGTLSSGFPGLQLQYRVDPQFLKPGERGWFLRYLALHSLQIDVWDSDSLMLIGSAAVELKHMLRQGRPAVQVSHELEVITTEYMQDASFTSRHDSKHSATSAISVYTTVKGILHLRLGNIGGSVWNTQSSPAVPPSFSHVLQPNDGPQGLAEGRVSALTLLQLNASNARRARRLPEINSELARLLQSSMREVGGAEEKEVRQRKIDRMAAVRQHEEQKDEPPFQDYTNVTSHRADRVQHLRDLRVIEAYRERCKTQEITNMLSQAITTKHTLYATLGTAEFFEFVLKNPFNVPQTVTIECDDPELSVLVKSEEWRYFKELTKTLTPLEEEMFHLKEKTLTPQVYLRPKESVHVPFKYQTFVCGHSFPLQDSKSLKISRSPQMAQKHLSNTIQAKSIKVLFRGEDRKPLAICQVDVEPTPHVVDQTFRFYQPELTFLKKAIRLPAAWDDTADGSASLHVHCSDPNVICHTTSLTPGEPQDVYLKVPGSPSPQIRKFFITIFTDPWLASPAQIWQVYVHYLQRMDLSCVCGALSWHSLLLRGTQAIRRVKCYTSHPLELQVDPAEVFALPAQGVQDVRVGLRALRAGSRFFYLNVVDVDTRQLVDSWLLCITCRQPVISKAFEISVPVEGGKGSNKKISFTNPYSSSRVFNLRTDRPDLLHFKEEQFQIGGGELYTIGLRFAPSQNPGMEEILIFINDKQDKNEDTYCVRVQYR

Target ORF information:

RefSeq Version	XM_021478419.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nephronophthisis 4 (nphp4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478419.1

ORF Insert Sequence:

ATGAGCCACA CGACGGGGTC TCCATGGCAA CAGCAGCTCC CCTCTCTGCT CTCTCCCTCC 
CCGATGGCAT CGGCCCACCA GCTGTCACAT GTCGAGCGTC CAGTTGTTGG CAGTATTGCT 
CATCTGGAGC TGAGCGTGCA GGAGAGAGTG GGAGACAGAA GTGAGGAAGA GGATGAGATG 
CAGGAGTTGA CCTTCACCCC GGTGCACGCG CCTGTCATCA CCCTCGGGAC AAACGCTGCT 
GGCTCCTCCA GATCGACCTC CCGGAGATCT TTGGCGCAGC TCTTCTCTGC TGGTTTTCCT 
CAGATCCTGG ACCATCAGAA CCAGGTGGCC GAGGTTCTGG ATCCTTCTGA GCCTGTGAAC 
TTCAACCCCC AGAGAGAAGA AGCAGATTCT CTGCAGACAA ACACACTCAT CCTGCAGTTC 
CTGGCCTTTA CCAGGGTCCC TCAATCTGGT GTGAATTCAG ACTGGCCGAG CTCTGTCCAT 
TTCACCTTCC AGCTTTACCG CTTTCCTCCT GTCACCACCC AGCGGCTGAA GCTGCTTGAC 
CAGGAGAAGA CCAGCAAGTT CAAAGGCAGC TCTGATTCCC CTTGTGTGCT TTCTCTGCTC 
AACAAAGACG GCACACTTAG CTCTGGTTTT CCTGGTCTGC AGCTGCAGTA TCGAGTTGAC 
CCACAATTCC TAAAGCCAGG TGAACGGGGA TGGTTTCTGC GATACCTGGC ACTCCACTCA 
CTGCAGATTG ATGTCTGGGA CAGTGATTCC TTAATGCTGA TTGGCTCAGC TGCTGTGGAG 
CTGAAGCATA TGCTACGTCA AGGCCGTCCG GCTGTCCAGG TGAGCCATGA GCTAGAAGTG 
ATCACTACCG AGTACATGCA GGATGCCAGC TTTACTAGCA GACACGACAG CAAACACAGC 
GCCACTTCAG CCATCAGTGT CTACACTACT GTGAAAGGGA TTTTGCATCT ACGGCTCGGC 
AACATTGGTG GCAGTGTGTG GAATACTCAG AGTTCTCCAG CTGTTCCTCC GTCGTTCTCT 
CATGTTCTCC AGCCCAACGA TGGCCCTCAG GGTTTAGCAG AAGGAAGAGT GTCTGCTTTA 
ACACTCCTCC AGCTTAACGC GAGCAATGCC AGACGAGCCC GTCGGTTGCC AGAAATCAAC 
AGTGAACTGG CCAGACTCTT GCAAAGCAGC ATGAGGGAAG TGGGCGGAGC CGAAGAGAAG 
GAAGTGCGCC AGAGGAAGAT TGACAGAATG GCAGCTGTCC GTCAACACGA GGAGCAGAAA 
GACGAGCCCC CTTTTCAGGA CTACACAAAC GTCACGAGTC ACCGGGCGGA TCGAGTGCAG 
CATTTGCGAG ACCTGCGTGT GATTGAGGCC TATCGTGAGC GCTGTAAAAC ACAGGAGATC 
ACCAACATGC TCTCTCAGGC CATCACCACC AAACACACAC TCTACGCCAC CCTTGGTACG 
GCAGAGTTCT TCGAATTTGT GCTCAAGAAC CCATTCAATG TTCCTCAGAC AGTCACCATT 
GAATGTGATG ACCCAGAGCT CAGTGTACTG GTGAAGAGCG AAGAGTGGAG ATATTTTAAG 
GAGTTGACGA AGACTCTGAC TCCTCTAGAG GAGGAGATGT TTCACCTGAA GGAGAAAACA 
CTGACTCCTC AAGTTTACCT GCGGCCAAAA GAAAGCGTTC ACGTTCCCTT CAAGTACCAG 
ACCTTCGTTT GTGGTCACAG CTTTCCTCTT CAGGACTCAA AAAGCTTAAA GATTTCAAGA 
AGTCCACAGA TGGCTCAGAA ACATCTGTCC AATACAATCC AAGCCAAATC CATCAAGGTG 
TTGTTTCGTG GTGAGGACAG AAAGCCTCTG GCGATCTGCC AGGTGGATGT TGAACCAACG 
CCACATGTGG TGGACCAGAC CTTCCGCTTC TACCAGCCTG AGCTCACCTT CTTAAAGAAG 
GCCATTCGCC TGCCTGCAGC CTGGGACGAC ACAGCAGATG GATCTGCCAG CCTCCATGTG 
CACTGCAGTG ACCCCAATGT CATCTGCCAC ACCACCAGCC TGACTCCAGG AGAACCACAG 
GATGTTTACC TGAAAGTACC TGGAAGCCCA AGTCCACAGA TTAGAAAGTT CTTCATCACC 
ATTTTTACTG ACCCCTGGCT GGCCTCCCCT GCGCAGATCT GGCAGGTTTA CGTGCATTAC 
TTGCAAAGGA TGGACCTGAG CTGTGTGTGT GGTGCTCTGA GCTGGCATTC GCTGCTTCTG 
CGTGGCACAC AAGCCATTCG CAGGGTCAAG TGCTACACGT CGCATCCCCT CGAGCTTCAG 
GTGGATCCAG CTGAAGTTTT CGCTCTGCCG GCTCAGGGAG TTCAGGATGT TCGTGTGGGC 
CTGAGGGCTC TGAGGGCTGG AAGTCGCTTC TTTTACCTCA ATGTTGTTGA TGTGGACACA 
CGGCAGCTCG TGGACTCCTG GCTGCTGTGC ATCACATGCC GACAGCCTGT AATCTCCAAG 
GCGTTCGAAA TCAGCGTTCC TGTAGAAGGA GGGAAGGGCA GCAATAAAAA AATCAGTTTC 
ACAAACCCCT ACTCGTCCTC CAGAGTCTTC AATCTGCGCA CCGACCGCCC GGACCTGCTA 
CACTTTAAAG AGGAGCAGTT TCAGATTGGA GGAGGAGAGC TCTACACCAT TGGCCTGCGC 
TTTGCTCCAA GTCAGAACCC CGGCATGGAA GAGATTCTCA TCTTCATAAA TGACAAACAG 
GACAAGAATG AGGACACTTA CTGTGTTCGA GTCCAGTACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_021472054.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021327729.1 Danio rerio nephronophthisis 4 (nphp4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa144980
Clone ID Related Accession (Same CDS sequence)	XM_021472054.1
Accession Version	XM_021472054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	nephrocystin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394722.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTGTG TGCGCTGGTG TGCCTGGAGT CTGTTTTGGG ATCCTGAGGG TCCCGGAGAA 
AACGTGTGCC TCAACCTGCA GGGCGACTCT TTCCCGAACC CTTGGGCTGT CATGGTCTAC 
ACCACAAGCA CACAGCAGAC AAATTCTGGG AAATGTGTCA TCAAATTGAA GTTTTCATCT 
AGTGCAGAAG GAGTTTCTAG CAGCGCGGCA TTGAAAGTGA GCCACCAAGA AACATCAGCA 
CCTAAGAAGT TACTTTCAGC TTCCCAAGAT GAAAGGATCT TTGAGTCGAC AGCTTCAGTG 
TTGCAGTCTT CACGTGGCCC AGCGCTGTCT CTCTCTCAGC TGGTTGCGAC TTCACGCTAC 
CCGACCATGA GCCACACGAC GGGGTCTCCA TGGCAACAGC AGCTCCCCTC TCTGCTCTCT 
CCCTCCCCGA TGGCATCGGC CCACCAGCTG TCACATGTCG AGCGTCCAGT TGTTGGCAGT 
ATTGCTCATC TGGAGCTGAG CGTGCAGGAG AGAGTGGGAG ACAGAAGTGA GGAAGAGGAT 
GAGATGCAGG AGTTGACCTT CACCCCGGTG CACGCGCCTG TCATCACCCT CGGGACAAAC 
GCTGCTGGCT CCTCCAGATC GACCTCCCGG AGATCTTTGG CGCAGCTCTT CTCTGCTGGT 
TTTCCTCAGA TCCTGGACCA TCAGAACCAG GTGGCCGAGG TTCTGGATCC TTCTGAGCCT 
GTGAACTTCA ACCCCCAGAG AGAAGAAGCA GATTCTCTGC AGGCAAACAC ACTCGTCCTG 
CAGTTCCTGG CCTTTACCAG GGTCCCTCAA TCTGGTGTGA ATTCAGACTG GCCGAGCTCT 
GTCCATTTCA CCTTCCAGCT TTACCGCTTT CCTCCTGTCA CCACCCAGCG GCTGAAGCTG 
CTTGACCAGG AGAAGACCAG CAAGTTCAAA GGCAGCTCTG ATTCCCCTTG TGTGCTTTCT 
CTGCTCAACA AAGACGGCAC ACTTAGCTCT GGTTTTCCTG GTCTGCAGCT GCAGTATCGA 
GTTGACCCAC AATTCCTAAA GCCAGGTGAA CGGGGATGGT TTCTGCGATA CCTGGCACTC 
CACTCACTGC AGATTGATGT CTGGGACAGT GATTCCTTAA TGCTGATTGG CTCAGCTGCT 
GTGGAGCTGA AGCATATGCT ACGTCAAGGC CGTCCGGCTG TCCAGGTGAG CCATGAGCTA 
GAAGTGATCA CTACCGAGTA CATGCAGGAT GCCAGCTTTA CTAGCAGACA CGACAGCAAA 
CACAGCGCCA CTTCAGCCAT CAGTGTCTAC ACTACTGTGA AAGGGATTTT GCATCTACGG 
CTCGGCAACA TTGGTGGCAG TGTGTGGAAT ACTCAGAGTT CTCCAGCTGT TCCTCCGTCG 
TTCTCTCATG TTCTCCAGCC CAACGATGGC CCTCAGGGTA TAGCAGGAGG AAGAGTGTCT 
GCTTTAACAC TCCTCCAGCT TAACGCGAGC AATGCCAGAC GAGCCCGTCG GTTGCCAGAA 
ATCAACAGTG AACTGGCCAG ACTCTTGCAA AGCAGCATGA GGGAAGTGGG CGGAGCCGAA 
GAGAAGGAAG TGCGCCAGAG GAAGATTGAC AGAATGGCAG CTGTCCGTCA ACTCGAGGAG 
CAGAAAGACG AGCCCCCTTT TCAGGACTAC ACAAACGTCA CGAGTCACCG GGCGGATCGA 
GTGCAGCATT TGCGAGACCT GCGTGTGATT GAGGCCTATC GTGAGCGCTG TAAAACACAG 
GAGATCACCA ACATGCTCTC TCAGGCCATC ACCACCAAAC ACACACTCTA CGCCACCCTT 
GGTACGGCAG AGTTCTTCGA ATTTGTGCTC AAGAACCCAT TCAATGTTCC TCAGACAGTC 
ACCATTGAAT GTGATGACCC AGAGCTCAGT GTACTGGTGA AGAGCGAAGA GTGGAGATAT 
TTTAAGGAGT TGACGAAGAC TCTGACTCCT CTAGAGGAGG AGATGTTTCA CCTGAAGGAG 
AAAACACTGA CTCCTCAAGT TTACCTGCGG CCAAAAGAAA GCGTTCACGT TCCCTTCAAG 
TACCAGACCT TCGTTTGTGG TCACAGCTTT CCTCTTCAGG ACTCAAAAAG CTTAAAGATT 
TCAAGAAGTC CACAGATGGC TCAGAAACAT CTGTCCAATA CAATCCAAGC CAAATCCATC 
AAGGTGTTGT TTCGTGGTGA GGACAGAAAG CCTCTGGCGA TCTGCCAGGT GGATGTTGAA 
CCAACGCCAC ATGTGGTGGA CCAGACCTTC CGCTTCTACC AGCCTGAGCT CACCTTCTTA 
AAGAAGGCCA TTCGCCTGCC TGCAGCCTGG GACGACACAG CAGATGGATC TGCCAGCCTC 
CATGTGCACT GCAGTGACCC CAATGTCATC TGCCACACCA CCAGCCTGAC TCCAGGAGAA 
CCACAGGATG TTTACCTGAA AGTACCTGGA AGCCCAAGTC CACAGATTAG AAAGTTCTTC 
ATCACCATTT TTACTGACCC CTGGCTGGCC TCCCCTGCGC AGATCTGGCA GGTTTACGTG 
CATTACTTGC AAAGGATGGA CCTGAGCTGT GTGTGTGGTG CTCTGAGCTG GCATTCGCTG 
CTTCTGCGTG GCACACAAGC CATTCGCAGG GTCAAGTGCT ACACGTCGCA TCCCCTCGAG 
CTTCAGGTGG ATCCAGCTGA AGTTTTCGCT CTGCCGGCTC AGGGAGTTCA GGATGTTCGT 
GTGGGCCTGA GGGCTCTGAG GGCTGGAAGT CGCTTCTTTT ACCTCAATGT TGTTGATGTG 
GACACACGGC AGCTCGTGGA CTCCTGGCTG CTGTGCATCA CATGCCGACA GCCTGTAATC 
TCCAAGGCGT TCGAAATCAG CGTTCCTGTA GAAGGAGGGA AGGGCAGCAA TAAAAAAATC 
AGTTTCACAA ACCCCTACTC GTCCTCCAGA GTCTTCAATC TGCGCACCGA CCGCCCGGAC 
CTGCTACACT TTAAAGAGGA GCAGTTTCAG ATTGGAGGAG GAGAGCTCTA CACCATTGGC 
CTGCGCTTTG CTCCAAGTCA GAACCCCGGC ATGGAAGAGA TTCTCATCTT CATAAATGAC 
AAACAGGACA AGAATGAGGA CACTTACTGT GTTCGAGTCC AGTACAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327729.1
CDS	51..3161
Translation	MQCVRWCAWSLFWDPEGPGENVCLNLQGDSFPNPWAVMVYTTSTQQTNSGKCVIKLKFSSSAEGVSSSAALKVSHQETSAPKKLLSASQDERIFESTASVLQSSRGPALSLSQLVATSRYPTMSHTTGSPWQQQLPSLLSPSPMASAHQLSHVERPVVGSIAHLELSVQERVGDRSEEEDEMQELTFTPVHAPVITLGTNAAGSSRSTSRRSLAQLFSAGFPQILDHQNQVAEVLDPSEPVNFNPQREEADSLQANTLVLQFLAFTRVPQSGVNSDWPSSVHFTFQLYRFPPVTTQRLKLLDQEKTSKFKGSSDSPCVLSLLNKDGTLSSGFPGLQLQYRVDPQFLKPGERGWFLRYLALHSLQIDVWDSDSLMLIGSAAVELKHMLRQGRPAVQVSHELEVITTEYMQDASFTSRHDSKHSATSAISVYTTVKGILHLRLGNIGGSVWNTQSSPAVPPSFSHVLQPNDGPQGIAGGRVSALTLLQLNASNARRARRLPEINSELARLLQSSMREVGGAEEKEVRQRKIDRMAAVRQLEEQKDEPPFQDYTNVTSHRADRVQHLRDLRVIEAYRERCKTQEITNMLSQAITTKHTLYATLGTAEFFEFVLKNPFNVPQTVTIECDDPELSVLVKSEEWRYFKELTKTLTPLEEEMFHLKEKTLTPQVYLRPKESVHVPFKYQTFVCGHSFPLQDSKSLKISRSPQMAQKHLSNTIQAKSIKVLFRGEDRKPLAICQVDVEPTPHVVDQTFRFYQPELTFLKKAIRLPAAWDDTADGSASLHVHCSDPNVICHTTSLTPGEPQDVYLKVPGSPSPQIRKFFITIFTDPWLASPAQIWQVYVHYLQRMDLSCVCGALSWHSLLLRGTQAIRRVKCYTSHPLELQVDPAEVFALPAQGVQDVRVGLRALRAGSRFFYLNVVDVDTRQLVDSWLLCITCRQPVISKAFEISVPVEGGKGSNKKISFTNPYSSSRVFNLRTDRPDLLHFKEEQFQIGGGELYTIGLRFAPSQNPGMEEILIFINDKQDKNEDTYCVRVQYR

Target ORF information:

RefSeq Version	XM_021472054.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nephronophthisis 4 (nphp4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472054.1

ORF Insert Sequence:

ATGCAGTGTG TGCGCTGGTG TGCCTGGAGT CTGTTTTGGG ATCCTGAGGG TCCCGGAGAA 
AACGTGTGCC TCAACCTGCA GGGCGACTCT TTCCCGAACC CTTGGGCTGT CATGGTCTAC 
ACCACAAGCA CACAGCAGAC AAATTCTGGG AAATGTGTCA TCAAATTGAA GTTTTCATCT 
AGTGCAGAAG GAGTTTCTAG CAGCGCGGCA TTGAAAGTGA GCCACCAAGA AACATCAGCA 
CCTAAGAAGT TACTTTCAGC TTCCCAAGAT GAAAGGATCT TTGAGTCGAC AGCTTCAGTG 
TTGCAGTCTT CACGTGGCCC AGCGCTGTCT CTCTCTCAGC TGGTTGCGAC TTCACGCTAC 
CCGACCATGA GCCACACGAC GGGGTCTCCA TGGCAACAGC AGCTCCCCTC TCTGCTCTCT 
CCCTCCCCGA TGGCATCGGC CCACCAGCTG TCACATGTCG AGCGTCCAGT TGTTGGCAGT 
ATTGCTCATC TGGAGCTGAG CGTGCAGGAG AGAGTGGGAG ACAGAAGTGA GGAAGAGGAT 
GAGATGCAGG AGTTGACCTT CACCCCGGTG CACGCGCCTG TCATCACCCT CGGGACAAAC 
GCTGCTGGCT CCTCCAGATC GACCTCCCGG AGATCTTTGG CGCAGCTCTT CTCTGCTGGT 
TTTCCTCAGA TCCTGGACCA TCAGAACCAG GTGGCCGAGG TTCTGGATCC TTCTGAGCCT 
GTGAACTTCA ACCCCCAGAG AGAAGAAGCA GATTCTCTGC AGGCAAACAC ACTCGTCCTG 
CAGTTCCTGG CCTTTACCAG GGTCCCTCAA TCTGGTGTGA ATTCAGACTG GCCGAGCTCT 
GTCCATTTCA CCTTCCAGCT TTACCGCTTT CCTCCTGTCA CCACCCAGCG GCTGAAGCTG 
CTTGACCAGG AGAAGACCAG CAAGTTCAAA GGCAGCTCTG ATTCCCCTTG TGTGCTTTCT 
CTGCTCAACA AAGACGGCAC ACTTAGCTCT GGTTTTCCTG GTCTGCAGCT GCAGTATCGA 
GTTGACCCAC AATTCCTAAA GCCAGGTGAA CGGGGATGGT TTCTGCGATA CCTGGCACTC 
CACTCACTGC AGATTGATGT CTGGGACAGT GATTCCTTAA TGCTGATTGG CTCAGCTGCT 
GTGGAGCTGA AGCATATGCT ACGTCAAGGC CGTCCGGCTG TCCAGGTGAG CCATGAGCTA 
GAAGTGATCA CTACCGAGTA CATGCAGGAT GCCAGCTTTA CTAGCAGACA CGACAGCAAA 
CACAGCGCCA CTTCAGCCAT CAGTGTCTAC ACTACTGTGA AAGGGATTTT GCATCTACGG 
CTCGGCAACA TTGGTGGCAG TGTGTGGAAT ACTCAGAGTT CTCCAGCTGT TCCTCCGTCG 
TTCTCTCATG TTCTCCAGCC CAACGATGGC CCTCAGGGTA TAGCAGGAGG AAGAGTGTCT 
GCTTTAACAC TCCTCCAGCT TAACGCGAGC AATGCCAGAC GAGCCCGTCG GTTGCCAGAA 
ATCAACAGTG AACTGGCCAG ACTCTTGCAA AGCAGCATGA GGGAAGTGGG CGGAGCCGAA 
GAGAAGGAAG TGCGCCAGAG GAAGATTGAC AGAATGGCAG CTGTCCGTCA ACTCGAGGAG 
CAGAAAGACG AGCCCCCTTT TCAGGACTAC ACAAACGTCA CGAGTCACCG GGCGGATCGA 
GTGCAGCATT TGCGAGACCT GCGTGTGATT GAGGCCTATC GTGAGCGCTG TAAAACACAG 
GAGATCACCA ACATGCTCTC TCAGGCCATC ACCACCAAAC ACACACTCTA CGCCACCCTT 
GGTACGGCAG AGTTCTTCGA ATTTGTGCTC AAGAACCCAT TCAATGTTCC TCAGACAGTC 
ACCATTGAAT GTGATGACCC AGAGCTCAGT GTACTGGTGA AGAGCGAAGA GTGGAGATAT 
TTTAAGGAGT TGACGAAGAC TCTGACTCCT CTAGAGGAGG AGATGTTTCA CCTGAAGGAG 
AAAACACTGA CTCCTCAAGT TTACCTGCGG CCAAAAGAAA GCGTTCACGT TCCCTTCAAG 
TACCAGACCT TCGTTTGTGG TCACAGCTTT CCTCTTCAGG ACTCAAAAAG CTTAAAGATT 
TCAAGAAGTC CACAGATGGC TCAGAAACAT CTGTCCAATA CAATCCAAGC CAAATCCATC 
AAGGTGTTGT TTCGTGGTGA GGACAGAAAG CCTCTGGCGA TCTGCCAGGT GGATGTTGAA 
CCAACGCCAC ATGTGGTGGA CCAGACCTTC CGCTTCTACC AGCCTGAGCT CACCTTCTTA 
AAGAAGGCCA TTCGCCTGCC TGCAGCCTGG GACGACACAG CAGATGGATC TGCCAGCCTC 
CATGTGCACT GCAGTGACCC CAATGTCATC TGCCACACCA CCAGCCTGAC TCCAGGAGAA 
CCACAGGATG TTTACCTGAA AGTACCTGGA AGCCCAAGTC CACAGATTAG AAAGTTCTTC 
ATCACCATTT TTACTGACCC CTGGCTGGCC TCCCCTGCGC AGATCTGGCA GGTTTACGTG 
CATTACTTGC AAAGGATGGA CCTGAGCTGT GTGTGTGGTG CTCTGAGCTG GCATTCGCTG 
CTTCTGCGTG GCACACAAGC CATTCGCAGG GTCAAGTGCT ACACGTCGCA TCCCCTCGAG 
CTTCAGGTGG ATCCAGCTGA AGTTTTCGCT CTGCCGGCTC AGGGAGTTCA GGATGTTCGT 
GTGGGCCTGA GGGCTCTGAG GGCTGGAAGT CGCTTCTTTT ACCTCAATGT TGTTGATGTG 
GACACACGGC AGCTCGTGGA CTCCTGGCTG CTGTGCATCA CATGCCGACA GCCTGTAATC 
TCCAAGGCGT TCGAAATCAG CGTTCCTGTA GAAGGAGGGA AGGGCAGCAA TAAAAAAATC 
AGTTTCACAA ACCCCTACTC GTCCTCCAGA GTCTTCAATC TGCGCACCGA CCGCCCGGAC 
CTGCTACACT TTAAAGAGGA GCAGTTTCAG ATTGGAGGAG GAGAGCTCTA CACCATTGGC 
CTGCGCTTTG CTCCAAGTCA GAACCCCGGC ATGGAAGAGA TTCTCATCTT CATAAATGAC 
AAACAGGACA AGAATGAGGA CACTTACTGT GTTCGAGTCC AGTACAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_005166952.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167009.2 Danio rerio nephronophthisis 4 (nphp4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43251
Clone ID Related Accession (Same CDS sequence)	XM_005166952.3
Accession Version	XM_005166952.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nephrocystin-4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGT GGAGAAGCGC TTTCCAGAGG AACCGCGTGA TTCCGCCGCA CAGTCAGACT 
GCTCGACAAG CGCCAGGATC TGCTCAGGGT CTGCAGCTGC TCTTCAAACA CCTCGATGGA 
CTTCACGTGA CACAGTCTGA GTCTCCGCCA TCACTTCAGT ATCAGCTGCG GGTGACCTTG 
TTTGACTCAG GTCACCAGCA CTTCTTCGGG CGCACATGGA AGAGCGGCTC ACACTCTGTC 
AGTGGCTCAC CGGGTCAGAG CTATCGAGTG CTCTTCAATG AGGTTGTGTA CTTTCACACT 
TCCCTGTGTT TGAGCTCAGT GATGGCAGTG GTAGAGCTGG TGTCTCTGTC TACCCGGGCA 
GACGGTACCC AGGAAGCAGT GGGGTCAGGT TTTGGACTCC TCCAGCTCTT CACAGGTCAC 
GCAGAGTCCA GCGTCTCTCA GGGGGAGGAC AGGCTGAATC TGTTCCATGG GACCCCCAGA 
GCTCTCCTGC ACCCAACACT GAAAGACCCT CTGCAGTTGA ATGCCATGTT GTCTGTGATG 
GAGGGCACTC AGCTGATCTA CTCCATACAG CCACATCTAG CTCTCACTAC TATCATGCAC 
CTTCTCCCAC CCAATGTTCT AGTGTCTGGA CATGAGAAAA TCCCTGGTGT GGTGTCACCA 
ACGGACACAG ATACAGCAGA TGCGCTGCGG AAGCCTCGTC TGCTGAAGAG CTTCTCGTGT 
GTGTTGGAGG GCCTACGTGT GACACTCTTC CCCTCCGTGG AGGTCTTTGA ATCTGACCTG 
CTCCAGCTGC TCAACAGAGA CTGCCAGAAT ACAAATAGGG GTGCAGAAGG AAGGTCAGTT 
GTGGTGCAGG AGCGCAGGCT GCTGGTTGGG GTTCATAATG GCTGGGGTTG GGTGGAGAAG 
CCTCAAGTTT TGGTGCTGGA ACGTGATTCT GGAGGAACTG AAGCAGCCAA GGCTCGTGGA 
TCCAGCTTCA GGCAGACCTC CACAGACAAA CTCAGTGATG GACTGTCTCT TCGTGGCGGT 
GTGCAGCTGA AGCTGATGAG GGACGTGGAT TTCAGCATTG TCTTCCAGCT CCAGTATGTT 
TTCTCATGTC CACTGGCTGT TGATGGGCAG CTGACATCCA CAGCTGTGCC CAGAGCAGCG 
TTTATGCAGT GTGTGCGCTG GTGTGCCTGG AGTCTGTTTT GGGATCCTGA GGGTCCCGGA 
GAAAACGTGT GCCTCAACCT GCAGGGCGAC TCTTTCCCGA ACCCTTGGGC TGTCATGGTC 
TACACCACAA GCACACAGCA GACAAATTCT GGGAAATGTG TCATCAAATT GAAGTTTTCA 
TCTAGTGCAG AAGGAGTTTC TAGCAGCGCG GCATTGAAAG TGAGCCACCA AGAAACATCA 
GCACCTAAGA AGTTACTTTC AGCTTCCCAA GATGAACGGA TCTTTGAGTC GACAGCTTCA 
GTGTTGCAGT CTTCACGTGG CCCAGCGCTG TCTCTCTCTC AGCTGGTTGC GACTTCACGC 
TACCCGACCA TGAGCCACAC GACGGGGTCT CCATGGCAAC AGCAGCTCCC CTCTCTGCTC 
TCTCCCTCCC CGATGGCATC GGCCCACCAG CTGTCACATG TCGAGCGTCC AGTTGTTGGC 
AGTATTGCTC ATCTGGAGCT GAGCGTGCAG GAGAGAGTGG GAGACAGAAG TGAGGAAGAG 
GATGAGATGC AGGAGTTGAC CTTCACCCCG GTGCACGCGC CTGTCATCAC CCTCGGGACA 
AACGCTGCTG GCTCCTCCAG ATCGACCTCC CGGAGATCTT TGGCGCAGCT CTTCTCTGCT 
GGTTTTCCTC AGATCCTGGA CCATCAGAAC CAGGTGGCCG AGGTTCTGGA TCCTTCTGAG 
CCTGTGAACT TCAACCCCCA GAGAGAAGAA GCAGATTCTC TGCAGACAAA CACACTCATC 
CTGCAGTTCC TGGCCTTTAC CAGGGTCCCT CAATCTGGTG TGAATTCAGA CTGGCCGAGC 
TCTGTCCATT TCACCTTCCA GCTTTACCGC TTTCCTCCTG TCACCACCCA GCGGCTGAAG 
CTGCTTGACC AGGAGAAGAC CAGCAAGTTC AAAGGCAGCT CTGATTCCCC TTGTGTGCTT 
TCTCTGCTCA ACAAAGACGG CACACTTAGC TCTGGTTTTC CTGGTCTGCA GCTGCAGTAT 
CGAGTTGACC CACAATTCCT AAAGCCAGGT GAACGGGGAT GGTTTCTGCG ATACCTGGCA 
CTCCACTCAC TGCAGATTGA TGTCTGGGAC AGTGATTCCT TAATGCTGAT TGGCTCAGCT 
GCTGTGGAGC TGAAGCATAT GCTACGTCAA GGCCGTCCGG CTGTCCAGGT GAGCCATGAG 
CTAGAAGTGA TCACTACCGA GTACATGCAG GATGCCAGCT TTACTAGCAG ACACGACAGC 
AAACACAGCG CCACTTCAGC CATCAGTGTC TACACTACTG TGAAAGGGAT TTTGCATCTA 
CGGCTCGGCA ACATTGGTGG CAGTGTGTGG AATACTCAGA GTTCTCCAGC TGTTCCTCCG 
TCGTTCTCTC ATGTTCTCCA GCCCAACGAT GGCCCTCAGG GTTTAGCAGA AGGAAGAGTG 
TCTGCTTTAA CACTCCTCCA GCTTAACGCG AGCAATGCCA GACGAGCCCG TCGGTTGCCA 
GAAATCAACA GTGAACTGGC CAGACTCTTG CAAAGCAGCA TGAGGGAAGT GGGCGGAGCC 
GAAGAGAAGG AAGTGCGCCA GAGGAAGATT GACAGAATGG CAGCTGTCCG TCAACACGAG 
GAGCAGAAAG ACGAGCCCCC TTTTCAGGAC TACACAAACG TCACGAGTCA CCGGGCGGAT 
CGAGTGCAGC ATTTGCGAGA CCTGCGTGTG ATTGAGGCCT ATCGTGAGCG CTGTAAAACA 
CAGGAGATCA CCAACATGCT CTCTCAGGCC ATCACCACCA AACACACACT CTACGCCACC 
CTTGGTACGG CAGAGTTCTT CGAATTTGTG CTCAAGAACC CATTCAATGT TCCTCAGACA 
GTCACCATTG AATGTGATGA CCCAGAGCTC AGTGTACTGG TGAAGAGCGA AGAGTGGAGA 
TATTTTAAGG AGTTGACGAA GACTCTGACT CCTCTAGAGG AGGAGATGTT TCACCTGAAG 
GAGAAAACAC TGACTCCTCA AGTTTACCTG CGGCCAAAAG AAAGCGTTCA CGTTCCCTTC 
AAGTACCAGA CCTTCGTTTG TGGTCACAGC TTTCCTCTTC AGGACTCAAA AAGCTTAAAG 
ATTTCAAGAA GTCCACAGAT GGCTCAGAAA CATCTGTCCA ATACAATCCA AGCCAAATCC 
ATCAAGGTGT TGTTTCGTGG TGAGGACAGA AAGCCTCTGG CGATCTGCCA GGTGGATGTT 
GAACCAACGC CACATGTGGT GGACCAGACC TTCCGCTTCT ACCAGCCTGA GCTCACCTTC 
TTAAAGAAGG CCATTCGCCT GCCTGCAGCC TGGGACGACA CAGCAGATGG ATCTGCCAGC 
CTCCATGTGC ACTGCAGTGA CCCCAATGTC ATCTGCCACA CCACCAGCCT GACTCCAGGA 
GAACCACAGG ATGTTTACCT GAAAGTACCT GGAAGCCCAA GTCCACAGAT TAGAAAGTTC 
TTCATCACCA TTTTTACTGA CCCCTGGCTG GCCTCCCCTG CGCAGATCTG GCAGGTTTAC 
GTGCATTACT TGCAAAGGAT GGACCTGAGC TGTGTGTGTG GTGCTCTGAG CTGGCATTCG 
CTGCTTCTGC GTGGCACACA AGCCATTCGC AGGGTCAAGT GCTACACGTC GCATCCCCTC 
GAGCTTCAGG TGGATCCAGC TGAAGTTTTC GCTCTGCCGG CTCAGGGAGT TCAGGATGTT 
CGTGTGGGCC TGAGGGCTCT GAGGGCTGGA AGTCGCTTCT TTTACCTCAA TGTTGTTGAT 
GTGGACACAC GGCAGCTCGT GGACTCCTGG CTGCTGTGCA TCACATGCCG ACAGCCTGTA 
ATCTCCAAGG CGTTCGAAAT CAGCGTTCCT GTAGAAGGAG GGAAGGGCAG CAATAAAAAA 
ATCAGTTTCA CAAACCCCTA CTCGTCCTCC AGAGTCTTCA ATCTGCGCAC CGACCGCCCG 
GACCTGCTAC ACTTTAAAGA GGAGCAGTTT CAGATTGGAG GAGGAGAGCT CTACACCATT 
GGCCTGCGCT TTGCTCCAAG TCAGAACCCC GGCATGGAAG AGATTCTCAT CTTCATAAAT 
GACAAACAGG ACAAGAATGA GGACACTTAC TGTGTTCGAG TCCAGTACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167009.2
CDS	559..4812
Translation	MSEWRSAFQRNRVIPPHSQTARQAPGSAQGLQLLFKHLDGLHVTQSESPPSLQYQLRVTLFDSGHQHFFGRTWKSGSHSVSGSPGQSYRVLFNEVVYFHTSLCLSSVMAVVELVSLSTRADGTQEAVGSGFGLLQLFTGHAESSVSQGEDRLNLFHGTPRALLHPTLKDPLQLNAMLSVMEGTQLIYSIQPHLALTTIMHLLPPNVLVSGHEKIPGVVSPTDTDTADALRKPRLLKSFSCVLEGLRVTLFPSVEVFESDLLQLLNRDCQNTNRGAEGRSVVVQERRLLVGVHNGWGWVEKPQVLVLERDSGGTEAAKARGSSFRQTSTDKLSDGLSLRGGVQLKLMRDVDFSIVFQLQYVFSCPLAVDGQLTSTAVPRAAFMQCVRWCAWSLFWDPEGPGENVCLNLQGDSFPNPWAVMVYTTSTQQTNSGKCVIKLKFSSSAEGVSSSAALKVSHQETSAPKKLLSASQDERIFESTASVLQSSRGPALSLSQLVATSRYPTMSHTTGSPWQQQLPSLLSPSPMASAHQLSHVERPVVGSIAHLELSVQERVGDRSEEEDEMQELTFTPVHAPVITLGTNAAGSSRSTSRRSLAQLFSAGFPQILDHQNQVAEVLDPSEPVNFNPQREEADSLQTNTLILQFLAFTRVPQSGVNSDWPSSVHFTFQLYRFPPVTTQRLKLLDQEKTSKFKGSSDSPCVLSLLNKDGTLSSGFPGLQLQYRVDPQFLKPGERGWFLRYLALHSLQIDVWDSDSLMLIGSAAVELKHMLRQGRPAVQVSHELEVITTEYMQDASFTSRHDSKHSATSAISVYTTVKGILHLRLGNIGGSVWNTQSSPAVPPSFSHVLQPNDGPQGLAEGRVSALTLLQLNASNARRARRLPEINSELARLLQSSMREVGGAEEKEVRQRKIDRMAAVRQHEEQKDEPPFQDYTNVTSHRADRVQHLRDLRVIEAYRERCKTQEITNMLSQAITTKHTLYATLGTAEFFEFVLKNPFNVPQTVTIECDDPELSVLVKSEEWRYFKELTKTLTPLEEEMFHLKEKTLTPQVYLRPKESVHVPFKYQTFVCGHSFPLQDSKSLKISRSPQMAQKHLSNTIQAKSIKVLFRGEDRKPLAICQVDVEPTPHVVDQTFRFYQPELTFLKKAIRLPAAWDDTADGSASLHVHCSDPNVICHTTSLTPGEPQDVYLKVPGSPSPQIRKFFITIFTDPWLASPAQIWQVYVHYLQRMDLSCVCGALSWHSLLLRGTQAIRRVKCYTSHPLELQVDPAEVFALPAQGVQDVRVGLRALRAGSRFFYLNVVDVDTRQLVDSWLLCITCRQPVISKAFEISVPVEGGKGSNKKISFTNPYSSSRVFNLRTDRPDLLHFKEEQFQIGGGELYTIGLRFAPSQNPGMEEILIFINDKQDKNEDTYCVRVQYR

Target ORF information:

RefSeq Version	XM_005166952.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nephronophthisis 4 (nphp4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166952.3

ORF Insert Sequence:

ATGTCTGAGT GGAGAAGCGC TTTCCAGAGG AACCGCGTGA TTCCGCCGCA CAGTCAGACT 
GCTCGACAAG CGCCAGGATC TGCTCAGGGT CTGCAGCTGC TCTTCAAACA CCTCGATGGA 
CTTCACGTGA CACAGTCTGA GTCTCCGCCA TCACTTCAGT ATCAGCTGCG GGTGACCTTG 
TTTGACTCAG GTCACCAGCA CTTCTTCGGG CGCACATGGA AGAGCGGCTC ACACTCTGTC 
AGTGGCTCAC CGGGTCAGAG CTATCGAGTG CTCTTCAATG AGGTTGTGTA CTTTCACACT 
TCCCTGTGTT TGAGCTCAGT GATGGCAGTG GTAGAGCTGG TGTCTCTGTC TACCCGGGCA 
GACGGTACCC AGGAAGCAGT GGGGTCAGGT TTTGGACTCC TCCAGCTCTT CACAGGTCAC 
GCAGAGTCCA GCGTCTCTCA GGGGGAGGAC AGGCTGAATC TGTTCCATGG GACCCCCAGA 
GCTCTCCTGC ACCCAACACT GAAAGACCCT CTGCAGTTGA ATGCCATGTT GTCTGTGATG 
GAGGGCACTC AGCTGATCTA CTCCATACAG CCACATCTAG CTCTCACTAC TATCATGCAC 
CTTCTCCCAC CCAATGTTCT AGTGTCTGGA CATGAGAAAA TCCCTGGTGT GGTGTCACCA 
ACGGACACAG ATACAGCAGA TGCGCTGCGG AAGCCTCGTC TGCTGAAGAG CTTCTCGTGT 
GTGTTGGAGG GCCTACGTGT GACACTCTTC CCCTCCGTGG AGGTCTTTGA ATCTGACCTG 
CTCCAGCTGC TCAACAGAGA CTGCCAGAAT ACAAATAGGG GTGCAGAAGG AAGGTCAGTT 
GTGGTGCAGG AGCGCAGGCT GCTGGTTGGG GTTCATAATG GCTGGGGTTG GGTGGAGAAG 
CCTCAAGTTT TGGTGCTGGA ACGTGATTCT GGAGGAACTG AAGCAGCCAA GGCTCGTGGA 
TCCAGCTTCA GGCAGACCTC CACAGACAAA CTCAGTGATG GACTGTCTCT TCGTGGCGGT 
GTGCAGCTGA AGCTGATGAG GGACGTGGAT TTCAGCATTG TCTTCCAGCT CCAGTATGTT 
TTCTCATGTC CACTGGCTGT TGATGGGCAG CTGACATCCA CAGCTGTGCC CAGAGCAGCG 
TTTATGCAGT GTGTGCGCTG GTGTGCCTGG AGTCTGTTTT GGGATCCTGA GGGTCCCGGA 
GAAAACGTGT GCCTCAACCT GCAGGGCGAC TCTTTCCCGA ACCCTTGGGC TGTCATGGTC 
TACACCACAA GCACACAGCA GACAAATTCT GGGAAATGTG TCATCAAATT GAAGTTTTCA 
TCTAGTGCAG AAGGAGTTTC TAGCAGCGCG GCATTGAAAG TGAGCCACCA AGAAACATCA 
GCACCTAAGA AGTTACTTTC AGCTTCCCAA GATGAACGGA TCTTTGAGTC GACAGCTTCA 
GTGTTGCAGT CTTCACGTGG CCCAGCGCTG TCTCTCTCTC AGCTGGTTGC GACTTCACGC 
TACCCGACCA TGAGCCACAC GACGGGGTCT CCATGGCAAC AGCAGCTCCC CTCTCTGCTC 
TCTCCCTCCC CGATGGCATC GGCCCACCAG CTGTCACATG TCGAGCGTCC AGTTGTTGGC 
AGTATTGCTC ATCTGGAGCT GAGCGTGCAG GAGAGAGTGG GAGACAGAAG TGAGGAAGAG 
GATGAGATGC AGGAGTTGAC CTTCACCCCG GTGCACGCGC CTGTCATCAC CCTCGGGACA 
AACGCTGCTG GCTCCTCCAG ATCGACCTCC CGGAGATCTT TGGCGCAGCT CTTCTCTGCT 
GGTTTTCCTC AGATCCTGGA CCATCAGAAC CAGGTGGCCG AGGTTCTGGA TCCTTCTGAG 
CCTGTGAACT TCAACCCCCA GAGAGAAGAA GCAGATTCTC TGCAGACAAA CACACTCATC 
CTGCAGTTCC TGGCCTTTAC CAGGGTCCCT CAATCTGGTG TGAATTCAGA CTGGCCGAGC 
TCTGTCCATT TCACCTTCCA GCTTTACCGC TTTCCTCCTG TCACCACCCA GCGGCTGAAG 
CTGCTTGACC AGGAGAAGAC CAGCAAGTTC AAAGGCAGCT CTGATTCCCC TTGTGTGCTT 
TCTCTGCTCA ACAAAGACGG CACACTTAGC TCTGGTTTTC CTGGTCTGCA GCTGCAGTAT 
CGAGTTGACC CACAATTCCT AAAGCCAGGT GAACGGGGAT GGTTTCTGCG ATACCTGGCA 
CTCCACTCAC TGCAGATTGA TGTCTGGGAC AGTGATTCCT TAATGCTGAT TGGCTCAGCT 
GCTGTGGAGC TGAAGCATAT GCTACGTCAA GGCCGTCCGG CTGTCCAGGT GAGCCATGAG 
CTAGAAGTGA TCACTACCGA GTACATGCAG GATGCCAGCT TTACTAGCAG ACACGACAGC 
AAACACAGCG CCACTTCAGC CATCAGTGTC TACACTACTG TGAAAGGGAT TTTGCATCTA 
CGGCTCGGCA ACATTGGTGG CAGTGTGTGG AATACTCAGA GTTCTCCAGC TGTTCCTCCG 
TCGTTCTCTC ATGTTCTCCA GCCCAACGAT GGCCCTCAGG GTTTAGCAGA AGGAAGAGTG 
TCTGCTTTAA CACTCCTCCA GCTTAACGCG AGCAATGCCA GACGAGCCCG TCGGTTGCCA 
GAAATCAACA GTGAACTGGC CAGACTCTTG CAAAGCAGCA TGAGGGAAGT GGGCGGAGCC 
GAAGAGAAGG AAGTGCGCCA GAGGAAGATT GACAGAATGG CAGCTGTCCG TCAACACGAG 
GAGCAGAAAG ACGAGCCCCC TTTTCAGGAC TACACAAACG TCACGAGTCA CCGGGCGGAT 
CGAGTGCAGC ATTTGCGAGA CCTGCGTGTG ATTGAGGCCT ATCGTGAGCG CTGTAAAACA 
CAGGAGATCA CCAACATGCT CTCTCAGGCC ATCACCACCA AACACACACT CTACGCCACC 
CTTGGTACGG CAGAGTTCTT CGAATTTGTG CTCAAGAACC CATTCAATGT TCCTCAGACA 
GTCACCATTG AATGTGATGA CCCAGAGCTC AGTGTACTGG TGAAGAGCGA AGAGTGGAGA 
TATTTTAAGG AGTTGACGAA GACTCTGACT CCTCTAGAGG AGGAGATGTT TCACCTGAAG 
GAGAAAACAC TGACTCCTCA AGTTTACCTG CGGCCAAAAG AAAGCGTTCA CGTTCCCTTC 
AAGTACCAGA CCTTCGTTTG TGGTCACAGC TTTCCTCTTC AGGACTCAAA AAGCTTAAAG 
ATTTCAAGAA GTCCACAGAT GGCTCAGAAA CATCTGTCCA ATACAATCCA AGCCAAATCC 
ATCAAGGTGT TGTTTCGTGG TGAGGACAGA AAGCCTCTGG CGATCTGCCA GGTGGATGTT 
GAACCAACGC CACATGTGGT GGACCAGACC TTCCGCTTCT ACCAGCCTGA GCTCACCTTC 
TTAAAGAAGG CCATTCGCCT GCCTGCAGCC TGGGACGACA CAGCAGATGG ATCTGCCAGC 
CTCCATGTGC ACTGCAGTGA CCCCAATGTC ATCTGCCACA CCACCAGCCT GACTCCAGGA 
GAACCACAGG ATGTTTACCT GAAAGTACCT GGAAGCCCAA GTCCACAGAT TAGAAAGTTC 
TTCATCACCA TTTTTACTGA CCCCTGGCTG GCCTCCCCTG CGCAGATCTG GCAGGTTTAC 
GTGCATTACT TGCAAAGGAT GGACCTGAGC TGTGTGTGTG GTGCTCTGAG CTGGCATTCG 
CTGCTTCTGC GTGGCACACA AGCCATTCGC AGGGTCAAGT GCTACACGTC GCATCCCCTC 
GAGCTTCAGG TGGATCCAGC TGAAGTTTTC GCTCTGCCGG CTCAGGGAGT TCAGGATGTT 
CGTGTGGGCC TGAGGGCTCT GAGGGCTGGA AGTCGCTTCT TTTACCTCAA TGTTGTTGAT 
GTGGACACAC GGCAGCTCGT GGACTCCTGG CTGCTGTGCA TCACATGCCG ACAGCCTGTA 
ATCTCCAAGG CGTTCGAAAT CAGCGTTCCT GTAGAAGGAG GGAAGGGCAG CAATAAAAAA 
ATCAGTTTCA CAAACCCCTA CTCGTCCTCC AGAGTCTTCA ATCTGCGCAC CGACCGCCCG 
GACCTGCTAC ACTTTAAAGA GGAGCAGTTT CAGATTGGAG GAGGAGAGCT CTACACCATT 
GGCCTGCGCT TTGCTCCAAG TCAGAACCCC GGCATGGAAG AGATTCTCAT CTTCATAAAT 
GACAAACAGG ACAAGAATGA GGACACTTAC TGTGTTCGAG TCCAGTACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_017357449.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212938.1 Danio rerio nephronophthisis 4 (nphp4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa60047
Clone ID Related Accession (Same CDS sequence)	XM_017357449.1
Accession Version	XM_017357449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nephrocystin-4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGT GGAGAAGCGC TTTCCAGAGG AACCGCGTGA TTCCGCCGCA CAGTCAGACT 
GCTCGACAAG CGCCAGGATC TGCTCAGGGT CTGCAGCTGC TCTTCAAACA CCTCGATGGA 
CTTCACGTGA CACAGTCTGA GTCTCCGCCA TCACTTCAGT ATCAGCTGCG GGTGACCTTG 
TTTGACTCAG GTCACCAGCA CTTCTTCGGG CGCACATGGA AGAGCGGCTC ACACTCTGTC 
AGTGGCTCAC CGGGTCAGAG CTATCGAGTG CTCTTCAATG AGGTTGTGTA CTTTCACACT 
TCCCTGTGTT TGAGCTCAGT GATGGCAGTG GTAGAGCTGG TGTCTCTGTC TACCCGGGCA 
GACGGTACCC AGGAAGCAGT GGGGTCAGGT TTTGGACTCC TCCAGCTCTT CACAGGTCAC 
GCAGAGTCCA GCGTCTCTCA GGGGGAGGAC AGGCTGAATC TGTTCCATGG GACCCCCAGA 
GCTCTCCTGC ACCCAACACT GAAAGACCCT CTGCAGTTGA ATGCCATGTT GTCTGTGATG 
GAGGGCACTC AGCTGATCTA CTCCATACAG CCACATCTAG CTCTCACTAC TATCATGCAC 
CTTCTCCCAC CCAATGTTCT AGTGTCTGGA CATGAGAAAA TCCCTGGTGT GGTGTCACCA 
ACGGACACAG ATACAGCAGA TGCGCTGCGG AAGCCTCGTC TGCTGAAGAG CTTCTCGTGT 
GTGTTGGAGG GCCTACGTGT GACACTCTTC CCCTCCGTGG AGGTCTTTGA ATCTGACCTG 
CTCCAGCTGC TCAACAGAGA CTGCCAGAAT ACAAATAGGG GTGCAGAAGG AAGGTCAGTT 
GTGGTGCAGG AGCGCAGGCT GCTGGTTGGG GTTCATAATG GCTGGGGTTG GGTGGAGAAG 
CCTCAAGTTT TGGTGCTGGA ACGTGATTCT GGAGGAACTG AAGCAGCCAA GGCTCGTGGA 
TCCAGCTTCA GGCAGACCTC CACAGACAAA CTCAGCAGTG ATGGACTGTC TCTTCGTGGC 
GGTGTGCAGC TGAAGCTGAT GAGGGACGTG GATTTCAGCA TTGTCTTCCA GCTCCAGTAT 
GTTTTCTCAT GTCCACTGGC TGTTGATGGG CAGCTGACAT CCACAGCTGT GCCCAGAGCA 
GCGTTTATGC AGTGTGTGCG CTGGTGTGCC TGGAGTCTGT TTTGGGATCC TGAGGGTCCC 
GGAGAAAACG TGTGCCTCAA CCTGCAGGGC GACTCTTTCC CGAACCCTTG GGCTGTCATG 
GTCTACACCA CAAGCACACA GCAGACAAAT TCTGGGAAAT GTGTCATCAA ATTGAAGTTT 
TCATCTAGTG CAGAAGGAGT TTCTAGCAGC GCGGCATTGA AAGTGAGCCA CCAAGAAACA 
TCAGCACCTA AGAAGTTACT TTCAGCTTCC CAAGATGAAC GGATCTTTGA GTCGACAGCT 
TCAGTGTTGC AGTCTTCACG TGGCCCAGCG CTGTCTCTCT CTCAGCTGGT TGCGACTTCA 
CGCTACCCGA CCATGAGCCA CACGACGGGG TCTCCATGGC AACAGCAGCT CCCCTCTCTG 
CTCTCTCCCT CCCCGATGGC ATCGGCCCAC CAGCTGTCAC ATGTCGAGCG TCCAGTTGTT 
GGCAGTATTG CTCATCTGGA GCTGAGCGTG CAGGAGAGAG TGGGAGACAG AAGTGAGGAA 
GAGGATGAGA TGCAGGAGTT GACCTTCACC CCGGTGCACG CGCCTGTCAT CACCCTCGGG 
ACAAACGCTG CTGGCTCCTC CAGATCGACC TCCCGGAGAT CTTTGGCGCA GCTCTTCTCT 
GCTGGTTTTC CTCAGATCCT GGACCATCAG AACCAGGTGG CCGAGGTTCT GGATCCTTCT 
GAGCCTGTGA ACTTCAACCC CCAGAGAGAA GAAGCAGATT CTCTGCAGAC AAACACACTC 
ATCCTGCAGT TCCTGGCCTT TACCAGGGTC CCTCAATCTG GTGTGAATTC AGACTGGCCG 
AGCTCTGTCC ATTTCACCTT CCAGCTTTAC CGCTTTCCTC CTGTCACCAC CCAGCGGCTG 
AAGCTGCTTG ACCAGGAGAA GACCAGCAAG TTCAAAGGCA GCTCTGATTC CCCTTGTGTG 
CTTTCTCTGC TCAACAAAGA CGGCACACTT AGCTCTGGTT TTCCTGGTCT GCAGCTGCAG 
TATCGAGTTG ACCCACAATT CCTAAAGCCA GGTGAACGGG GATGGTTTCT GCGATACCTG 
GCACTCCACT CACTGCAGAT TGATGTCTGG GACAGTGATT CCTTAATGCT GATTGGCTCA 
GCTGCTGTGG AGCTGAAGCA TATGCTACGT CAAGGCCGTC CGGCTGTCCA GGTGAGCCAT 
GAGCTAGAAG TGATCACTAC CGAGTACATG CAGGATGCCA GCTTTACTAG CAGACACGAC 
AGCAAACACA GCGCCACTTC AGCCATCAGT GTCTACACTA CTGTGAAAGG GATTTTGCAT 
CTACGGCTCG GCAACATTGG TGGCAGTGTG TGGAATACTC AGAGTTCTCC AGCTGTTCCT 
CCGTCGTTCT CTCATGTTCT CCAGCCCAAC GATGGCCCTC AGGGTTTAGC AGAAGGAAGA 
GTGTCTGCTT TAACACTCCT CCAGCTTAAC GCGAGCAATG CCAGACGAGC CCGTCGGTTG 
CCAGAAATCA ACAGTGAACT GGCCAGACTC TTGCAAAGCA GCATGAGGGA AGTGGGCGGA 
GCCGAAGAGA AGGAAGTGCG CCAGAGGAAG ATTGACAGAA TGGCAGCTGT CCGTCAACAC 
GAGGAGCAGA AAGACGAGCC CCCTTTTCAG GACTACACAA ACGTCACGAG TCACCGGGCG 
GATCGAGTGC AGCATTTGCG AGACCTGCGT GTGATTGAGG CCTATCGTGA GCGCTGTAAA 
ACACAGGAGA TCACCAACAT GCTCTCTCAG GCCATCACCA CCAAACACAC ACTCTACGCC 
ACCCTTGGTA CGGCAGAGTT CTTCGAATTT GTGCTCAAGA ACCCATTCAA TGTTCCTCAG 
ACAGTCACCA TTGAATGTGA TGACCCAGAG CTCAGTGTAC TGGTGAAGAG CGAAGAGTGG 
AGATATTTTA AGGAGTTGAC GAAGACTCTG ACTCCTCTAG AGGAGGAGAT GTTTCACCTG 
AAGGAGAAAA CACTGACTCC TCAAGTTTAC CTGCGGCCAA AAGAAAGCGT TCACGTTCCC 
TTCAAGTACC AGACCTTCGT TTGTGGTCAC AGCTTTCCTC TTCAGGACTC AAAAAGCTTA 
AAGATTTCAA GAAGTCCACA GATGGCTCAG AAACATCTGT CCAATACAAT CCAAGCCAAA 
TCCATCAAGG TGTTGTTTCG TGGTGAGGAC AGAAAGCCTC TGGCGATCTG CCAGGTGGAT 
GTTGAACCAA CGCCACATGT GGTGGACCAG ACCTTCCGCT TCTACCAGCC TGAGCTCACC 
TTCTTAAAGA AGGCCATTCG CCTGCCTGCA GCCTGGGACG ACACAGCAGA TGGATCTGCC 
AGCCTCCATG TGCACTGCAG TGACCCCAAT GTCATCTGCC ACACCACCAG CCTGACTCCA 
GGAGAACCAC AGGATGTTTA CCTGAAAGTA CCTGGAAGCC CAAGTCCACA GATTAGAAAG 
TTCTTCATCA CCATTTTTAC TGACCCCTGG CTGGCCTCCC CTGCGCAGAT CTGGCAGGTT 
TACGTGCATT ACTTGCAAAG GATGGACCTG AGCTGTGTGT GTGGTGCTCT GAGCTGGCAT 
TCGCTGCTTC TGCGTGGCAC ACAAGCCATT CGCAGGGTCA AGTGCTACAC GTCGCATCCC 
CTCGAGCTTC AGGTGGATCC AGCTGAAGTT TTCGCTCTGC CGGCTCAGGG AGTTCAGGAT 
GTTCGTGTGG GCCTGAGGGC TCTGAGGGCT GGAAGTCGCT TCTTTTACCT CAATGTTGTT 
GATGTGGACA CACGGCAGCT CGTGGACTCC TGGCTGCTGT GCATCACATG CCGACAGCCT 
GTAATCTCCA AGGCGTTCGA AATCAGCGTT CCTGTAGAAG GAGGGAAGGG CAGCAATAAA 
AAAATCAGTT TCACAAACCC CTACTCGTCC TCCAGAGTCT TCAATCTGCG CACCGACCGC 
CCGGACCTGC TACACTTTAA AGAGGAGCAG TTTCAGATTG GAGGAGGAGA GCTCTACACC 
ATTGGCCTGC GCTTTGCTCC AAGTCAGAAC CCCGGCATGG AAGAGATTCT CATCTTCATA 
AATGACAAAC AGGACAAGAA TGAGGACACT TACTGTGTTC GAGTCCAGTA CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212938.1
CDS	559..4815
Translation	MSEWRSAFQRNRVIPPHSQTARQAPGSAQGLQLLFKHLDGLHVTQSESPPSLQYQLRVTLFDSGHQHFFGRTWKSGSHSVSGSPGQSYRVLFNEVVYFHTSLCLSSVMAVVELVSLSTRADGTQEAVGSGFGLLQLFTGHAESSVSQGEDRLNLFHGTPRALLHPTLKDPLQLNAMLSVMEGTQLIYSIQPHLALTTIMHLLPPNVLVSGHEKIPGVVSPTDTDTADALRKPRLLKSFSCVLEGLRVTLFPSVEVFESDLLQLLNRDCQNTNRGAEGRSVVVQERRLLVGVHNGWGWVEKPQVLVLERDSGGTEAAKARGSSFRQTSTDKLSSDGLSLRGGVQLKLMRDVDFSIVFQLQYVFSCPLAVDGQLTSTAVPRAAFMQCVRWCAWSLFWDPEGPGENVCLNLQGDSFPNPWAVMVYTTSTQQTNSGKCVIKLKFSSSAEGVSSSAALKVSHQETSAPKKLLSASQDERIFESTASVLQSSRGPALSLSQLVATSRYPTMSHTTGSPWQQQLPSLLSPSPMASAHQLSHVERPVVGSIAHLELSVQERVGDRSEEEDEMQELTFTPVHAPVITLGTNAAGSSRSTSRRSLAQLFSAGFPQILDHQNQVAEVLDPSEPVNFNPQREEADSLQTNTLILQFLAFTRVPQSGVNSDWPSSVHFTFQLYRFPPVTTQRLKLLDQEKTSKFKGSSDSPCVLSLLNKDGTLSSGFPGLQLQYRVDPQFLKPGERGWFLRYLALHSLQIDVWDSDSLMLIGSAAVELKHMLRQGRPAVQVSHELEVITTEYMQDASFTSRHDSKHSATSAISVYTTVKGILHLRLGNIGGSVWNTQSSPAVPPSFSHVLQPNDGPQGLAEGRVSALTLLQLNASNARRARRLPEINSELARLLQSSMREVGGAEEKEVRQRKIDRMAAVRQHEEQKDEPPFQDYTNVTSHRADRVQHLRDLRVIEAYRERCKTQEITNMLSQAITTKHTLYATLGTAEFFEFVLKNPFNVPQTVTIECDDPELSVLVKSEEWRYFKELTKTLTPLEEEMFHLKEKTLTPQVYLRPKESVHVPFKYQTFVCGHSFPLQDSKSLKISRSPQMAQKHLSNTIQAKSIKVLFRGEDRKPLAICQVDVEPTPHVVDQTFRFYQPELTFLKKAIRLPAAWDDTADGSASLHVHCSDPNVICHTTSLTPGEPQDVYLKVPGSPSPQIRKFFITIFTDPWLASPAQIWQVYVHYLQRMDLSCVCGALSWHSLLLRGTQAIRRVKCYTSHPLELQVDPAEVFALPAQGVQDVRVGLRALRAGSRFFYLNVVDVDTRQLVDSWLLCITCRQPVISKAFEISVPVEGGKGSNKKISFTNPYSSSRVFNLRTDRPDLLHFKEEQFQIGGGELYTIGLRFAPSQNPGMEEILIFINDKQDKNEDTYCVRVQYR

Target ORF information:

RefSeq Version	XM_017357449.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nephronophthisis 4 (nphp4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357449.1

ORF Insert Sequence:

ATGTCTGAGT GGAGAAGCGC TTTCCAGAGG AACCGCGTGA TTCCGCCGCA CAGTCAGACT 
GCTCGACAAG CGCCAGGATC TGCTCAGGGT CTGCAGCTGC TCTTCAAACA CCTCGATGGA 
CTTCACGTGA CACAGTCTGA GTCTCCGCCA TCACTTCAGT ATCAGCTGCG GGTGACCTTG 
TTTGACTCAG GTCACCAGCA CTTCTTCGGG CGCACATGGA AGAGCGGCTC ACACTCTGTC 
AGTGGCTCAC CGGGTCAGAG CTATCGAGTG CTCTTCAATG AGGTTGTGTA CTTTCACACT 
TCCCTGTGTT TGAGCTCAGT GATGGCAGTG GTAGAGCTGG TGTCTCTGTC TACCCGGGCA 
GACGGTACCC AGGAAGCAGT GGGGTCAGGT TTTGGACTCC TCCAGCTCTT CACAGGTCAC 
GCAGAGTCCA GCGTCTCTCA GGGGGAGGAC AGGCTGAATC TGTTCCATGG GACCCCCAGA 
GCTCTCCTGC ACCCAACACT GAAAGACCCT CTGCAGTTGA ATGCCATGTT GTCTGTGATG 
GAGGGCACTC AGCTGATCTA CTCCATACAG CCACATCTAG CTCTCACTAC TATCATGCAC 
CTTCTCCCAC CCAATGTTCT AGTGTCTGGA CATGAGAAAA TCCCTGGTGT GGTGTCACCA 
ACGGACACAG ATACAGCAGA TGCGCTGCGG AAGCCTCGTC TGCTGAAGAG CTTCTCGTGT 
GTGTTGGAGG GCCTACGTGT GACACTCTTC CCCTCCGTGG AGGTCTTTGA ATCTGACCTG 
CTCCAGCTGC TCAACAGAGA CTGCCAGAAT ACAAATAGGG GTGCAGAAGG AAGGTCAGTT 
GTGGTGCAGG AGCGCAGGCT GCTGGTTGGG GTTCATAATG GCTGGGGTTG GGTGGAGAAG 
CCTCAAGTTT TGGTGCTGGA ACGTGATTCT GGAGGAACTG AAGCAGCCAA GGCTCGTGGA 
TCCAGCTTCA GGCAGACCTC CACAGACAAA CTCAGCAGTG ATGGACTGTC TCTTCGTGGC 
GGTGTGCAGC TGAAGCTGAT GAGGGACGTG GATTTCAGCA TTGTCTTCCA GCTCCAGTAT 
GTTTTCTCAT GTCCACTGGC TGTTGATGGG CAGCTGACAT CCACAGCTGT GCCCAGAGCA 
GCGTTTATGC AGTGTGTGCG CTGGTGTGCC TGGAGTCTGT TTTGGGATCC TGAGGGTCCC 
GGAGAAAACG TGTGCCTCAA CCTGCAGGGC GACTCTTTCC CGAACCCTTG GGCTGTCATG 
GTCTACACCA CAAGCACACA GCAGACAAAT TCTGGGAAAT GTGTCATCAA ATTGAAGTTT 
TCATCTAGTG CAGAAGGAGT TTCTAGCAGC GCGGCATTGA AAGTGAGCCA CCAAGAAACA 
TCAGCACCTA AGAAGTTACT TTCAGCTTCC CAAGATGAAC GGATCTTTGA GTCGACAGCT 
TCAGTGTTGC AGTCTTCACG TGGCCCAGCG CTGTCTCTCT CTCAGCTGGT TGCGACTTCA 
CGCTACCCGA CCATGAGCCA CACGACGGGG TCTCCATGGC AACAGCAGCT CCCCTCTCTG 
CTCTCTCCCT CCCCGATGGC ATCGGCCCAC CAGCTGTCAC ATGTCGAGCG TCCAGTTGTT 
GGCAGTATTG CTCATCTGGA GCTGAGCGTG CAGGAGAGAG TGGGAGACAG AAGTGAGGAA 
GAGGATGAGA TGCAGGAGTT GACCTTCACC CCGGTGCACG CGCCTGTCAT CACCCTCGGG 
ACAAACGCTG CTGGCTCCTC CAGATCGACC TCCCGGAGAT CTTTGGCGCA GCTCTTCTCT 
GCTGGTTTTC CTCAGATCCT GGACCATCAG AACCAGGTGG CCGAGGTTCT GGATCCTTCT 
GAGCCTGTGA ACTTCAACCC CCAGAGAGAA GAAGCAGATT CTCTGCAGAC AAACACACTC 
ATCCTGCAGT TCCTGGCCTT TACCAGGGTC CCTCAATCTG GTGTGAATTC AGACTGGCCG 
AGCTCTGTCC ATTTCACCTT CCAGCTTTAC CGCTTTCCTC CTGTCACCAC CCAGCGGCTG 
AAGCTGCTTG ACCAGGAGAA GACCAGCAAG TTCAAAGGCA GCTCTGATTC CCCTTGTGTG 
CTTTCTCTGC TCAACAAAGA CGGCACACTT AGCTCTGGTT TTCCTGGTCT GCAGCTGCAG 
TATCGAGTTG ACCCACAATT CCTAAAGCCA GGTGAACGGG GATGGTTTCT GCGATACCTG 
GCACTCCACT CACTGCAGAT TGATGTCTGG GACAGTGATT CCTTAATGCT GATTGGCTCA 
GCTGCTGTGG AGCTGAAGCA TATGCTACGT CAAGGCCGTC CGGCTGTCCA GGTGAGCCAT 
GAGCTAGAAG TGATCACTAC CGAGTACATG CAGGATGCCA GCTTTACTAG CAGACACGAC 
AGCAAACACA GCGCCACTTC AGCCATCAGT GTCTACACTA CTGTGAAAGG GATTTTGCAT 
CTACGGCTCG GCAACATTGG TGGCAGTGTG TGGAATACTC AGAGTTCTCC AGCTGTTCCT 
CCGTCGTTCT CTCATGTTCT CCAGCCCAAC GATGGCCCTC AGGGTTTAGC AGAAGGAAGA 
GTGTCTGCTT TAACACTCCT CCAGCTTAAC GCGAGCAATG CCAGACGAGC CCGTCGGTTG 
CCAGAAATCA ACAGTGAACT GGCCAGACTC TTGCAAAGCA GCATGAGGGA AGTGGGCGGA 
GCCGAAGAGA AGGAAGTGCG CCAGAGGAAG ATTGACAGAA TGGCAGCTGT CCGTCAACAC 
GAGGAGCAGA AAGACGAGCC CCCTTTTCAG GACTACACAA ACGTCACGAG TCACCGGGCG 
GATCGAGTGC AGCATTTGCG AGACCTGCGT GTGATTGAGG CCTATCGTGA GCGCTGTAAA 
ACACAGGAGA TCACCAACAT GCTCTCTCAG GCCATCACCA CCAAACACAC ACTCTACGCC 
ACCCTTGGTA CGGCAGAGTT CTTCGAATTT GTGCTCAAGA ACCCATTCAA TGTTCCTCAG 
ACAGTCACCA TTGAATGTGA TGACCCAGAG CTCAGTGTAC TGGTGAAGAG CGAAGAGTGG 
AGATATTTTA AGGAGTTGAC GAAGACTCTG ACTCCTCTAG AGGAGGAGAT GTTTCACCTG 
AAGGAGAAAA CACTGACTCC TCAAGTTTAC CTGCGGCCAA AAGAAAGCGT TCACGTTCCC 
TTCAAGTACC AGACCTTCGT TTGTGGTCAC AGCTTTCCTC TTCAGGACTC AAAAAGCTTA 
AAGATTTCAA GAAGTCCACA GATGGCTCAG AAACATCTGT CCAATACAAT CCAAGCCAAA 
TCCATCAAGG TGTTGTTTCG TGGTGAGGAC AGAAAGCCTC TGGCGATCTG CCAGGTGGAT 
GTTGAACCAA CGCCACATGT GGTGGACCAG ACCTTCCGCT TCTACCAGCC TGAGCTCACC 
TTCTTAAAGA AGGCCATTCG CCTGCCTGCA GCCTGGGACG ACACAGCAGA TGGATCTGCC 
AGCCTCCATG TGCACTGCAG TGACCCCAAT GTCATCTGCC ACACCACCAG CCTGACTCCA 
GGAGAACCAC AGGATGTTTA CCTGAAAGTA CCTGGAAGCC CAAGTCCACA GATTAGAAAG 
TTCTTCATCA CCATTTTTAC TGACCCCTGG CTGGCCTCCC CTGCGCAGAT CTGGCAGGTT 
TACGTGCATT ACTTGCAAAG GATGGACCTG AGCTGTGTGT GTGGTGCTCT GAGCTGGCAT 
TCGCTGCTTC TGCGTGGCAC ACAAGCCATT CGCAGGGTCA AGTGCTACAC GTCGCATCCC 
CTCGAGCTTC AGGTGGATCC AGCTGAAGTT TTCGCTCTGC CGGCTCAGGG AGTTCAGGAT 
GTTCGTGTGG GCCTGAGGGC TCTGAGGGCT GGAAGTCGCT TCTTTTACCT CAATGTTGTT 
GATGTGGACA CACGGCAGCT CGTGGACTCC TGGCTGCTGT GCATCACATG CCGACAGCCT 
GTAATCTCCA AGGCGTTCGA AATCAGCGTT CCTGTAGAAG GAGGGAAGGG CAGCAATAAA 
AAAATCAGTT TCACAAACCC CTACTCGTCC TCCAGAGTCT TCAATCTGCG CACCGACCGC 
CCGGACCTGC TACACTTTAA AGAGGAGCAG TTTCAGATTG GAGGAGGAGA GCTCTACACC 
ATTGGCCTGC GCTTTGCTCC AAGTCAGAAC CCCGGCATGG AAGAGATTCT CATCTTCATA 
AATGACAAAC AGGACAAGAA TGAGGACACT TACTGTGTTC GAGTCCAGTA CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPHP4 ( XM_017357450.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212939.1 Danio rerio nephronophthisis 4 (nphp4), transcript variant X3, mRNA.

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CloneID	ODa60048
Clone ID Related Accession (Same CDS sequence)	XM_017357450.1
Accession Version	XM_017357450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nephrocystin-4 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGT GGAGAAGCGC TTTCCAGAGG AACCGCGTGA TTCCGCCGCA CAGTCAGACT 
GCTCGACAAG CGCCAGGATC TGCTCAGGGT CTGCAGCTGC TCTTCAAACA CCTCGATGGA 
CTTCACGTGA CACAGTCTGA GTCTCCGCCA TCACTTCAGT ATCAGCTGCG GGTGACCTTG 
TTTGACTCAG GTCACCAGCA CTTCTTCGGG CGCACATGGA AGAGCGGCTC ACACTCTGTC 
AGTGGCTCAC CGGGTCAGAG CTATCGAGTG CTCTTCAATG AGGTTGTGTA CTTTCACACT 
TCCCTGTGTT TGAGCTCAGT GATGGCAGTG GTAGAGCTGG TGTCTCTGTC TACCCGGGCA 
GACGGTACCC AGGAAGCAGT GGGGTCAGGT TTTGGACTCC TCCAGCTCTT CACAGGTCAC 
GCAGAGTCCA GCGTCTCTCA GGGGGAGGAC AGGCTGAATC TGTTCCATGG GACCCCCAGA 
GCTCTCCTGC ACCCAACACT GAAAGACCCT CTGCAGTTGA ATGCCATGTT GTCTGTGATG 
GAGGGCACTC AGCTGATCTA CTCCATACAG CCACATCTAG CTCTCACTAC TATCATGCAC 
CTTCTCCCAC CCAATGTTCT AGTGTCTGGA CATGAGAAAA TCCCTGGTGT GGTGTCACCA 
ACGGACACAG ATACAGATGC GCTGCGGAAG CCTCGTCTGC TGAAGAGCTT CTCGTGTGTG 
TTGGAGGGCC TACGTGTGAC ACTCTTCCCC TCCGTGGAGG TCTTTGAATC TGACCTGCTC 
CAGCTGCTCA ACAGAGACTG CCAGAATACA AATAGGGGTG CAGAAGGAAG GTCAGTTGTG 
GTGCAGGAGC GCAGGCTGCT GGTTGGGGTT CATAATGGCT GGGGTTGGGT GGAGAAGCCT 
CAAGTTTTGG TGCTGGAACG TGATTCTGGA GGAACTGAAG CAGCCAAGGC TCGTGGATCC 
AGCTTCAGGC AGACCTCCAC AGACAAACTC AGCAGTGATG GACTGTCTCT TCGTGGCGGT 
GTGCAGCTGA AGCTGATGAG GGACGTGGAT TTCAGCATTG TCTTCCAGCT CCAGTATGTT 
TTCTCATGTC CACTGGCTGT TGATGGGCAG CTGACATCCA CAGCTGTGCC CAGAGCAGCG 
TTTATGCAGT GTGTGCGCTG GTGTGCCTGG AGTCTGTTTT GGGATCCTGA GGGTCCCGGA 
GAAAACGTGT GCCTCAACCT GCAGGGCGAC TCTTTCCCGA ACCCTTGGGC TGTCATGGTC 
TACACCACAA GCACACAGCA GACAAATTCT GGGAAATGTG TCATCAAATT GAAGTTTTCA 
TCTAGTGCAG AAGGAGTTTC TAGCAGCGCG GCATTGAAAG TGAGCCACCA AGAAACATCA 
GCACCTAAGA AGTTACTTTC AGCTTCCCAA GATGAACGGA TCTTTGAGTC GACAGCTTCA 
GTGTTGCAGT CTTCACGTGG CCCAGCGCTG TCTCTCTCTC AGCTGGTTGC GACTTCACGC 
TACCCGACCA TGAGCCACAC GACGGGGTCT CCATGGCAAC AGCAGCTCCC CTCTCTGCTC 
TCTCCCTCCC CGATGGCATC GGCCCACCAG CTGTCACATG TCGAGCGTCC AGTTGTTGGC 
AGTATTGCTC ATCTGGAGCT GAGCGTGCAG GAGAGAGTGG GAGACAGAAG TGAGGAAGAG 
GATGAGATGC AGGAGTTGAC CTTCACCCCG GTGCACGCGC CTGTCATCAC CCTCGGGACA 
AACGCTGCTG GCTCCTCCAG ATCGACCTCC CGGAGATCTT TGGCGCAGCT CTTCTCTGCT 
GGTTTTCCTC AGATCCTGGA CCATCAGAAC CAGGTGGCCG AGGTTCTGGA TCCTTCTGAG 
CCTGTGAACT TCAACCCCCA GAGAGAAGAA GCAGATTCTC TGCAGACAAA CACACTCATC 
CTGCAGTTCC TGGCCTTTAC CAGGGTCCCT CAATCTGGTG TGAATTCAGA CTGGCCGAGC 
TCTGTCCATT TCACCTTCCA GCTTTACCGC TTTCCTCCTG TCACCACCCA GCGGCTGAAG 
CTGCTTGACC AGGAGAAGAC CAGCAAGTTC AAAGGCAGCT CTGATTCCCC TTGTGTGCTT 
TCTCTGCTCA ACAAAGACGG CACACTTAGC TCTGGTTTTC CTGGTCTGCA GCTGCAGTAT 
CGAGTTGACC CACAATTCCT AAAGCCAGGT GAACGGGGAT GGTTTCTGCG ATACCTGGCA 
CTCCACTCAC TGCAGATTGA TGTCTGGGAC AGTGATTCCT TAATGCTGAT TGGCTCAGCT 
GCTGTGGAGC TGAAGCATAT GCTACGTCAA GGCCGTCCGG CTGTCCAGGT GAGCCATGAG 
CTAGAAGTGA TCACTACCGA GTACATGCAG GATGCCAGCT TTACTAGCAG ACACGACAGC 
AAACACAGCG CCACTTCAGC CATCAGTGTC TACACTACTG TGAAAGGGAT TTTGCATCTA 
CGGCTCGGCA ACATTGGTGG CAGTGTGTGG AATACTCAGA GTTCTCCAGC TGTTCCTCCG 
TCGTTCTCTC ATGTTCTCCA GCCCAACGAT GGCCCTCAGG GTTTAGCAGA AGGAAGAGTG 
TCTGCTTTAA CACTCCTCCA GCTTAACGCG AGCAATGCCA GACGAGCCCG TCGGTTGCCA 
GAAATCAACA GTGAACTGGC CAGACTCTTG CAAAGCAGCA TGAGGGAAGT GGGCGGAGCC 
GAAGAGAAGG AAGTGCGCCA GAGGAAGATT GACAGAATGG CAGCTGTCCG TCAACACGAG 
GAGCAGAAAG ACGAGCCCCC TTTTCAGGAC TACACAAACG TCACGAGTCA CCGGGCGGAT 
CGAGTGCAGC ATTTGCGAGA CCTGCGTGTG ATTGAGGCCT ATCGTGAGCG CTGTAAAACA 
CAGGAGATCA CCAACATGCT CTCTCAGGCC ATCACCACCA AACACACACT CTACGCCACC 
CTTGGTACGG CAGAGTTCTT CGAATTTGTG CTCAAGAACC CATTCAATGT TCCTCAGACA 
GTCACCATTG AATGTGATGA CCCAGAGCTC AGTGTACTGG TGAAGAGCGA AGAGTGGAGA 
TATTTTAAGG AGTTGACGAA GACTCTGACT CCTCTAGAGG AGGAGATGTT TCACCTGAAG 
GAGAAAACAC TGACTCCTCA AGTTTACCTG CGGCCAAAAG AAAGCGTTCA CGTTCCCTTC 
AAGTACCAGA CCTTCGTTTG TGGTCACAGC TTTCCTCTTC AGGACTCAAA AAGCTTAAAG 
ATTTCAAGAA GTCCACAGAT GGCTCAGAAA CATCTGTCCA ATACAATCCA AGCCAAATCC 
ATCAAGGTGT TGTTTCGTGG TGAGGACAGA AAGCCTCTGG CGATCTGCCA GGTGGATGTT 
GAACCAACGC CACATGTGGT GGACCAGACC TTCCGCTTCT ACCAGCCTGA GCTCACCTTC 
TTAAAGAAGG CCATTCGCCT GCCTGCAGCC TGGGACGACA CAGCAGATGG ATCTGCCAGC 
CTCCATGTGC ACTGCAGTGA CCCCAATGTC ATCTGCCACA CCACCAGCCT GACTCCAGGA 
GAACCACAGG ATGTTTACCT GAAAGTACCT GGAAGCCCAA GTCCACAGAT TAGAAAGTTC 
TTCATCACCA TTTTTACTGA CCCCTGGCTG GCCTCCCCTG CGCAGATCTG GCAGGTTTAC 
GTGCATTACT TGCAAAGGAT GGACCTGAGC TGTGTGTGTG GTGCTCTGAG CTGGCATTCG 
CTGCTTCTGC GTGGCACACA AGCCATTCGC AGGGTCAAGT GCTACACGTC GCATCCCCTC 
GAGCTTCAGG TGGATCCAGC TGAAGTTTTC GCTCTGCCGG CTCAGGGAGT TCAGGATGTT 
CGTGTGGGCC TGAGGGCTCT GAGGGCTGGA AGTCGCTTCT TTTACCTCAA TGTTGTTGAT 
GTGGACACAC GGCAGCTCGT GGACTCCTGG CTGCTGTGCA TCACATGCCG ACAGCCTGTA 
ATCTCCAAGG CGTTCGAAAT CAGCGTTCCT GTAGAAGGAG GGAAGGGCAG CAATAAAAAA 
ATCAGTTTCA CAAACCCCTA CTCGTCCTCC AGAGTCTTCA ATCTGCGCAC CGACCGCCCG 
GACCTGCTAC ACTTTAAAGA GGAGCAGTTT CAGATTGGAG GAGGAGAGCT CTACACCATT 
GGCCTGCGCT TTGCTCCAAG TCAGAACCCC GGCATGGAAG AGATTCTCAT CTTCATAAAT 
GACAAACAGG ACAAGAATGA GGACACTTAC TGTGTTCGAG TCCAGTACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212939.1
CDS	559..4812
Translation	MSEWRSAFQRNRVIPPHSQTARQAPGSAQGLQLLFKHLDGLHVTQSESPPSLQYQLRVTLFDSGHQHFFGRTWKSGSHSVSGSPGQSYRVLFNEVVYFHTSLCLSSVMAVVELVSLSTRADGTQEAVGSGFGLLQLFTGHAESSVSQGEDRLNLFHGTPRALLHPTLKDPLQLNAMLSVMEGTQLIYSIQPHLALTTIMHLLPPNVLVSGHEKIPGVVSPTDTDTDALRKPRLLKSFSCVLEGLRVTLFPSVEVFESDLLQLLNRDCQNTNRGAEGRSVVVQERRLLVGVHNGWGWVEKPQVLVLERDSGGTEAAKARGSSFRQTSTDKLSSDGLSLRGGVQLKLMRDVDFSIVFQLQYVFSCPLAVDGQLTSTAVPRAAFMQCVRWCAWSLFWDPEGPGENVCLNLQGDSFPNPWAVMVYTTSTQQTNSGKCVIKLKFSSSAEGVSSSAALKVSHQETSAPKKLLSASQDERIFESTASVLQSSRGPALSLSQLVATSRYPTMSHTTGSPWQQQLPSLLSPSPMASAHQLSHVERPVVGSIAHLELSVQERVGDRSEEEDEMQELTFTPVHAPVITLGTNAAGSSRSTSRRSLAQLFSAGFPQILDHQNQVAEVLDPSEPVNFNPQREEADSLQTNTLILQFLAFTRVPQSGVNSDWPSSVHFTFQLYRFPPVTTQRLKLLDQEKTSKFKGSSDSPCVLSLLNKDGTLSSGFPGLQLQYRVDPQFLKPGERGWFLRYLALHSLQIDVWDSDSLMLIGSAAVELKHMLRQGRPAVQVSHELEVITTEYMQDASFTSRHDSKHSATSAISVYTTVKGILHLRLGNIGGSVWNTQSSPAVPPSFSHVLQPNDGPQGLAEGRVSALTLLQLNASNARRARRLPEINSELARLLQSSMREVGGAEEKEVRQRKIDRMAAVRQHEEQKDEPPFQDYTNVTSHRADRVQHLRDLRVIEAYRERCKTQEITNMLSQAITTKHTLYATLGTAEFFEFVLKNPFNVPQTVTIECDDPELSVLVKSEEWRYFKELTKTLTPLEEEMFHLKEKTLTPQVYLRPKESVHVPFKYQTFVCGHSFPLQDSKSLKISRSPQMAQKHLSNTIQAKSIKVLFRGEDRKPLAICQVDVEPTPHVVDQTFRFYQPELTFLKKAIRLPAAWDDTADGSASLHVHCSDPNVICHTTSLTPGEPQDVYLKVPGSPSPQIRKFFITIFTDPWLASPAQIWQVYVHYLQRMDLSCVCGALSWHSLLLRGTQAIRRVKCYTSHPLELQVDPAEVFALPAQGVQDVRVGLRALRAGSRFFYLNVVDVDTRQLVDSWLLCITCRQPVISKAFEISVPVEGGKGSNKKISFTNPYSSSRVFNLRTDRPDLLHFKEEQFQIGGGELYTIGLRFAPSQNPGMEEILIFINDKQDKNEDTYCVRVQYR

Target ORF information:

RefSeq Version	XM_017357450.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nephronophthisis 4 (nphp4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357450.1

ORF Insert Sequence:

ATGTCTGAGT GGAGAAGCGC TTTCCAGAGG AACCGCGTGA TTCCGCCGCA CAGTCAGACT 
GCTCGACAAG CGCCAGGATC TGCTCAGGGT CTGCAGCTGC TCTTCAAACA CCTCGATGGA 
CTTCACGTGA CACAGTCTGA GTCTCCGCCA TCACTTCAGT ATCAGCTGCG GGTGACCTTG 
TTTGACTCAG GTCACCAGCA CTTCTTCGGG CGCACATGGA AGAGCGGCTC ACACTCTGTC 
AGTGGCTCAC CGGGTCAGAG CTATCGAGTG CTCTTCAATG AGGTTGTGTA CTTTCACACT 
TCCCTGTGTT TGAGCTCAGT GATGGCAGTG GTAGAGCTGG TGTCTCTGTC TACCCGGGCA 
GACGGTACCC AGGAAGCAGT GGGGTCAGGT TTTGGACTCC TCCAGCTCTT CACAGGTCAC 
GCAGAGTCCA GCGTCTCTCA GGGGGAGGAC AGGCTGAATC TGTTCCATGG GACCCCCAGA 
GCTCTCCTGC ACCCAACACT GAAAGACCCT CTGCAGTTGA ATGCCATGTT GTCTGTGATG 
GAGGGCACTC AGCTGATCTA CTCCATACAG CCACATCTAG CTCTCACTAC TATCATGCAC 
CTTCTCCCAC CCAATGTTCT AGTGTCTGGA CATGAGAAAA TCCCTGGTGT GGTGTCACCA 
ACGGACACAG ATACAGATGC GCTGCGGAAG CCTCGTCTGC TGAAGAGCTT CTCGTGTGTG 
TTGGAGGGCC TACGTGTGAC ACTCTTCCCC TCCGTGGAGG TCTTTGAATC TGACCTGCTC 
CAGCTGCTCA ACAGAGACTG CCAGAATACA AATAGGGGTG CAGAAGGAAG GTCAGTTGTG 
GTGCAGGAGC GCAGGCTGCT GGTTGGGGTT CATAATGGCT GGGGTTGGGT GGAGAAGCCT 
CAAGTTTTGG TGCTGGAACG TGATTCTGGA GGAACTGAAG CAGCCAAGGC TCGTGGATCC 
AGCTTCAGGC AGACCTCCAC AGACAAACTC AGCAGTGATG GACTGTCTCT TCGTGGCGGT 
GTGCAGCTGA AGCTGATGAG GGACGTGGAT TTCAGCATTG TCTTCCAGCT CCAGTATGTT 
TTCTCATGTC CACTGGCTGT TGATGGGCAG CTGACATCCA CAGCTGTGCC CAGAGCAGCG 
TTTATGCAGT GTGTGCGCTG GTGTGCCTGG AGTCTGTTTT GGGATCCTGA GGGTCCCGGA 
GAAAACGTGT GCCTCAACCT GCAGGGCGAC TCTTTCCCGA ACCCTTGGGC TGTCATGGTC 
TACACCACAA GCACACAGCA GACAAATTCT GGGAAATGTG TCATCAAATT GAAGTTTTCA 
TCTAGTGCAG AAGGAGTTTC TAGCAGCGCG GCATTGAAAG TGAGCCACCA AGAAACATCA 
GCACCTAAGA AGTTACTTTC AGCTTCCCAA GATGAACGGA TCTTTGAGTC GACAGCTTCA 
GTGTTGCAGT CTTCACGTGG CCCAGCGCTG TCTCTCTCTC AGCTGGTTGC GACTTCACGC 
TACCCGACCA TGAGCCACAC GACGGGGTCT CCATGGCAAC AGCAGCTCCC CTCTCTGCTC 
TCTCCCTCCC CGATGGCATC GGCCCACCAG CTGTCACATG TCGAGCGTCC AGTTGTTGGC 
AGTATTGCTC ATCTGGAGCT GAGCGTGCAG GAGAGAGTGG GAGACAGAAG TGAGGAAGAG 
GATGAGATGC AGGAGTTGAC CTTCACCCCG GTGCACGCGC CTGTCATCAC CCTCGGGACA 
AACGCTGCTG GCTCCTCCAG ATCGACCTCC CGGAGATCTT TGGCGCAGCT CTTCTCTGCT 
GGTTTTCCTC AGATCCTGGA CCATCAGAAC CAGGTGGCCG AGGTTCTGGA TCCTTCTGAG 
CCTGTGAACT TCAACCCCCA GAGAGAAGAA GCAGATTCTC TGCAGACAAA CACACTCATC 
CTGCAGTTCC TGGCCTTTAC CAGGGTCCCT CAATCTGGTG TGAATTCAGA CTGGCCGAGC 
TCTGTCCATT TCACCTTCCA GCTTTACCGC TTTCCTCCTG TCACCACCCA GCGGCTGAAG 
CTGCTTGACC AGGAGAAGAC CAGCAAGTTC AAAGGCAGCT CTGATTCCCC TTGTGTGCTT 
TCTCTGCTCA ACAAAGACGG CACACTTAGC TCTGGTTTTC CTGGTCTGCA GCTGCAGTAT 
CGAGTTGACC CACAATTCCT AAAGCCAGGT GAACGGGGAT GGTTTCTGCG ATACCTGGCA 
CTCCACTCAC TGCAGATTGA TGTCTGGGAC AGTGATTCCT TAATGCTGAT TGGCTCAGCT 
GCTGTGGAGC TGAAGCATAT GCTACGTCAA GGCCGTCCGG CTGTCCAGGT GAGCCATGAG 
CTAGAAGTGA TCACTACCGA GTACATGCAG GATGCCAGCT TTACTAGCAG ACACGACAGC 
AAACACAGCG CCACTTCAGC CATCAGTGTC TACACTACTG TGAAAGGGAT TTTGCATCTA 
CGGCTCGGCA ACATTGGTGG CAGTGTGTGG AATACTCAGA GTTCTCCAGC TGTTCCTCCG 
TCGTTCTCTC ATGTTCTCCA GCCCAACGAT GGCCCTCAGG GTTTAGCAGA AGGAAGAGTG 
TCTGCTTTAA CACTCCTCCA GCTTAACGCG AGCAATGCCA GACGAGCCCG TCGGTTGCCA 
GAAATCAACA GTGAACTGGC CAGACTCTTG CAAAGCAGCA TGAGGGAAGT GGGCGGAGCC 
GAAGAGAAGG AAGTGCGCCA GAGGAAGATT GACAGAATGG CAGCTGTCCG TCAACACGAG 
GAGCAGAAAG ACGAGCCCCC TTTTCAGGAC TACACAAACG TCACGAGTCA CCGGGCGGAT 
CGAGTGCAGC ATTTGCGAGA CCTGCGTGTG ATTGAGGCCT ATCGTGAGCG CTGTAAAACA 
CAGGAGATCA CCAACATGCT CTCTCAGGCC ATCACCACCA AACACACACT CTACGCCACC 
CTTGGTACGG CAGAGTTCTT CGAATTTGTG CTCAAGAACC CATTCAATGT TCCTCAGACA 
GTCACCATTG AATGTGATGA CCCAGAGCTC AGTGTACTGG TGAAGAGCGA AGAGTGGAGA 
TATTTTAAGG AGTTGACGAA GACTCTGACT CCTCTAGAGG AGGAGATGTT TCACCTGAAG 
GAGAAAACAC TGACTCCTCA AGTTTACCTG CGGCCAAAAG AAAGCGTTCA CGTTCCCTTC 
AAGTACCAGA CCTTCGTTTG TGGTCACAGC TTTCCTCTTC AGGACTCAAA AAGCTTAAAG 
ATTTCAAGAA GTCCACAGAT GGCTCAGAAA CATCTGTCCA ATACAATCCA AGCCAAATCC 
ATCAAGGTGT TGTTTCGTGG TGAGGACAGA AAGCCTCTGG CGATCTGCCA GGTGGATGTT 
GAACCAACGC CACATGTGGT GGACCAGACC TTCCGCTTCT ACCAGCCTGA GCTCACCTTC 
TTAAAGAAGG CCATTCGCCT GCCTGCAGCC TGGGACGACA CAGCAGATGG ATCTGCCAGC 
CTCCATGTGC ACTGCAGTGA CCCCAATGTC ATCTGCCACA CCACCAGCCT GACTCCAGGA 
GAACCACAGG ATGTTTACCT GAAAGTACCT GGAAGCCCAA GTCCACAGAT TAGAAAGTTC 
TTCATCACCA TTTTTACTGA CCCCTGGCTG GCCTCCCCTG CGCAGATCTG GCAGGTTTAC 
GTGCATTACT TGCAAAGGAT GGACCTGAGC TGTGTGTGTG GTGCTCTGAG CTGGCATTCG 
CTGCTTCTGC GTGGCACACA AGCCATTCGC AGGGTCAAGT GCTACACGTC GCATCCCCTC 
GAGCTTCAGG TGGATCCAGC TGAAGTTTTC GCTCTGCCGG CTCAGGGAGT TCAGGATGTT 
CGTGTGGGCC TGAGGGCTCT GAGGGCTGGA AGTCGCTTCT TTTACCTCAA TGTTGTTGAT 
GTGGACACAC GGCAGCTCGT GGACTCCTGG CTGCTGTGCA TCACATGCCG ACAGCCTGTA 
ATCTCCAAGG CGTTCGAAAT CAGCGTTCCT GTAGAAGGAG GGAAGGGCAG CAATAAAAAA 
ATCAGTTTCA CAAACCCCTA CTCGTCCTCC AGAGTCTTCA ATCTGCGCAC CGACCGCCCG 
GACCTGCTAC ACTTTAAAGA GGAGCAGTTT CAGATTGGAG GAGGAGAGCT CTACACCATT 
GGCCTGCGCT TTGCTCCAAG TCAGAACCCC GGCATGGAAG AGATTCTCAT CTTCATAAAT 
GACAAACAGG ACAAGAATGA GGACACTTAC TGTGTTCGAG TCCAGTACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NPHP4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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