ORF » Species Summary » Danio rerio » huwe1 cDNA ORF clone

huwe1 cDNA ORF clone, Danio rerio(zebrafish)

The following huwe1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the huwe1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa27855 NM_001365654.1
Latest version!		 Danio rerio HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa43094 NM_001365655.1
Latest version!		 Danio rerio HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_005162278.4
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_009296962.2
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_021473705.1
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_021473704.1
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_021473706.1
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_005162279.3
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27856 XM_021473707.1
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27856 XM_005162280.4
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27857 XM_021473708.1
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27857 XM_005162281.4
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27858 XM_021473709.1
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27858 XM_001923865.6
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27859 XM_021473710.1
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27859 XM_005162282.4
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa43094 XM_009296963.3
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa43094 XM_021473711.1
Latest version!		 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa43094 XM_009296963.2 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_005162279.2 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27858 XM_001923865.5 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27856 XM_005162280.3 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_005162278.3 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27857 XM_005162281.3 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27859 XM_005162282.3 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27855 XM_009296962.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    huwe1 ( NM_001365654.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352583.1 Danio rerio HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_021473704.1 , XM_009296962.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version NM_001365654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1554566400000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX664745.13. On or before Sep 12, 2018 this sequence version replaced XM_021473705.1, XM_005162279.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01013033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END## 

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    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352583.1
    CDS207..13916
    Translation

    Target ORF information:

    RefSeq Version NM_001365654.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001365654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( NM_001365655.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352584.1 Danio rerio HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID ODa43094
    Clone ID Related Accession (Same CDS sequence) XM_009296963.2 , XM_009296963.3 , XM_021473711.1 , NM_001365655.1
    Accession Version NM_001365655.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1554566400000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX664745.13. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01013032.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476746, SAMEA4476747 [ECO:0000348] ##Evidence-Data-END## 

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
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    3181
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    4081
    4141
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    4261
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    4381
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    4681
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    5281
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    5401
    5461
    5521
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    5821
    5881
    5941
    6001
    6061
    6121
    6181
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    6301
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    6421
    6481
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    7021
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    7141
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    7501
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    9061
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    9301
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    9421
    9481
    9541
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    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
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    10741
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    10861
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    10981
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    11101
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    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA 
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC 
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC 
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT 
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG 
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT 
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC 
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC 
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC 
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT 
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG 
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA 
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG 
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA 
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC 
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT 
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA 
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT 
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC 
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG 
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG 
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG 
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT 
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG 
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT 
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT 
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC 
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC 
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC 
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352584.1
    CDS244..13779
    Translation

    Target ORF information:

    RefSeq Version NM_001365655.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 (huwe1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001365655.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA 
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC 
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC 
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT 
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG 
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT 
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC 
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC 
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC 
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT 
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG 
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA 
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG 
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA 
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC 
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT 
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA 
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT 
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC 
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG 
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG 
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG 
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT 
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG 
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT 
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT 
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC 
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC 
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC 
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_021473705.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329380.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_021473704.1 , XM_009296962.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_021473705.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    2641
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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329380.1
    CDS214..13923
    Translation

    Target ORF information:

    RefSeq Version XM_021473705.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021473705.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_005162278.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162335.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_021473704.1 , XM_009296962.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_005162278.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162278.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    541
    601
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    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3601
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    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4681
    4741
    4801
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    4921
    4981
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    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
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    7081
    7141
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    7261
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    7681
    7741
    7801
    7861
    7921
    7981
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    8341
    8401
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    8521
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    8641
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    9061
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    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
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    10861
    10921
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    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162335.1
    CDS149..13858
    Translation

    Target ORF information:

    RefSeq Version XM_005162278.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005162278.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_005162279.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162336.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_021473704.1 , XM_009296962.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_005162279.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162279.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162336.1
    CDS214..13923
    Translation

    Target ORF information:

    RefSeq Version XM_005162279.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005162279.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_005162279.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162336.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_021473704.1 , XM_009296962.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_005162279.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005162279.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162336.1
    CDS221..13930
    Translation

    Target ORF information:

    RefSeq Version XM_005162279.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005162279.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_021473706.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329381.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_021473704.1 , XM_009296962.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_021473706.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329381.1
    CDS239..13948
    Translation

    Target ORF information:

    RefSeq Version XM_021473706.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021473706.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_021473704.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329379.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_021473704.1 , XM_009296962.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_021473704.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    13081
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    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329379.1
    CDS142..13851
    Translation

    Target ORF information:

    RefSeq Version XM_021473704.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021473704.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_009296962.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295237.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_021473704.1 , XM_009296962.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_009296962.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    2281
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    2521
    2581
    2641
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    11941
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    12061
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    12181
    12241
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    12361
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    12481
    12541
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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295237.1
    CDS258..13967
    Translation

    Target ORF information:

    RefSeq Version XM_009296962.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009296962.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_005162278.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162335.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_021473704.1 , XM_009296962.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_005162278.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162278.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162335.1
    CDS142..13851
    Translation

    Target ORF information:

    RefSeq Version XM_005162278.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005162278.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_009296962.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295237.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa27855
    Clone ID Related Accession (Same CDS sequence) XM_021473705.1 , XM_005162278.3 , XM_005162279.3 , XM_005162279.2 , XM_021473706.1 , XM_021473704.1 , XM_009296962.1 , NM_001365654.1 , XM_005162278.4 , XM_009296962.2
    Accession Version XM_009296962.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296962.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295237.1
    CDS239..13948
    Translation

    Target ORF information:

    RefSeq Version XM_009296962.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009296962.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG GTGAGAGAGG GACAGAGGGC ACGCCGTCTA 
    CAGCAGGCCC CCCCACCCTC TTCATCTGCA TCCTCTTCCG CTGCTGCTGC TGCTGCATCC 
    GCTGCTGTGG CTGTGGCTGG ATCGTCTGCC CCCAGTACCT CTGCAACCAA CAACCCCCCC 
    GCTGGCACGG CCAGCGCAGC CACGTCGGAG GTTCACACTG TTGCCGAGCC ACCAGCAGCT 
    CAGAGAGATG ATTCGCCCAT GGATGTGGAT CAGCCCTCTC CTTTGGAACA GGACCAGCCA 
    ACATTAGGTG AGGAGAGCAG CAGTCAGCAG GAGCAGGAGG AGCGTTTGCC TGACCTGCCG 
    CTGTTAAGTG AGCAGCTGCT TTTGGATGAG CTGTGGGACA TGCTTGGAGA ATGTCTGAAA 
    GAGCTTGAAG AGTCTCATGA CCAACATGCA GTTCTAGTGC TGCAGCCTGC AGTGGAGGCC 
    TTTTTCTTGG TGCATGCAAC AGAGCGGGAA AGCAAACCAC CAGTTCGGGA CACTCGGGAG 
    AGCCAGCTGT CACACATCAA GGATGAGCCT CCACCCCTGT CACCCGCCCC TCTCACACCC 
    GCTACCCCCT CCTCTCTGGA CCCCTTTTTT TCCAGAGAGC CTTCCTCTAT GCACATCTCT 
    TCCAACCTGC CTCCAGATAC ACAGAAGTTC CTCCGTTTCG CAGAGACCCA CCGCACAGTG 
    CTGAATCAGA TCCTGCGTCA ATCCACCACT CACCTGGCAG ACGGGCCTTT CGCTGTTTTG 
    GTTGACTACA TTCGCATCTT GGACTTTGAC GTCAAGCGCA AGTATTTCCG TCAGGAGCTG 
    GAGCGTCTGG ATGAAGGCTT GAGGAAAGAG GATATGGCCG TCCATGTGAG GAGAGATCAC 
    GTCTTTGAAG ATTCTTACAG AGAGCTGCAC CGCAAGAGCC CAGAAGACAT GAAGAACAGA 
    TTGTATATAG TATTTGAAGG AGAGGAGGGT CAGGATGCAG GCGGGCTGTT AAGAGAGTGG 
    TACATGATCA TCTCCAGAGA AATGTTCAAC CCCATGTATG CCCTGTTTCG CACCTCCCCC 
    GGCGATCGGG TCACCTATAC CATCAACCCC TCCTCCCACT GCAACCCCAA CCATCTCAGC 
    TACTTCAAGT TTGTGGGACG TGTGGTGGCC AAAGCAGTCT ACGACAACAG GCTATTAGAG 
    TGTTACTTTA CACGCAGCTT CTACAAGCAC ATCCTGGGCA AGAGCGTTAG GTATACAGAC 
    ATGGAAAGCG AAGACTATCC CTTTTTCCAA GGTCTGGTTT ATTTGTTGGA GAACGATGTG 
    TCGACTCTGG GCTACGAGCT GACATTCAGC ACAGAGGTCC AAGAGTTTGG CGTGTGTGAA 
    GTGAGAGATC TGAAGGCCAA CGGTGCCAAC ATTCTTGTCA CAGAAGAGAA TAAGAAGGAA 
    TATGTGCATC TGGTCTGCCA GATGAAGATG ACAGGTGCAA TCCGTAAACA GCTGGCTGCC 
    TTCCTGGAGG GCTTTTATGA GATCATCCCC AAGAGACTGA TCTCCATCTT CACTGAGCAA 
    GAGCTGGAGC TGCTCATCTC CGGCCTGCCC ACCATTGACA TCGACGACCT CAAGGCCAAT 
    ACTGAATACC ATAAATATCA GTCCAGTTCT ATTCAGATCC AGTGGTTCTG GCGTGCTTTA 
    CGATCCTTCG ACCAGGCTGA CAGGGCCAAA TTTCTGCAGT TTGTTACGGG CACTTCCAAG 
    GTGCCACTGC AGGGCTTTGC TGCACTGGAG GGAATGAACG GCATTCAGAA GTTCCAGATC 
    CACCGAGATG ACCGCTCCAC TGACCGCCTG CCCTCTGCGC ACACTTGCTT TAACCAGTTG 
    GACCTCCCAG CGTACGAGAG CTACGAGAAG TTAAGACACA TGCTGCTGCT GGCCATTCAG 
    GAATGTTCTG AAGGCTTCGG ACTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_021473707.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329382.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa27856
    Clone ID Related Accession (Same CDS sequence) XM_021473707.1 , XM_005162280.3 , XM_005162280.4
    Accession Version XM_021473707.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGAGAGAGG AGAGGAGCGG GCAAACGCTG TACCAGGGAA TCAAGACCTG 
    TGTCAGATCC TGCAGGATGT CGGTGATGAT GTCTACCAGC AGTACAGACT CACCAGACAA 
    GGCAGTGATT TTGACTCGCA ATCTGCCTTC CCCATCAATA CTCAGGTGTT TGCTGCAGAT 
    GGTGCTGTAG CTGAATCTTC TCAGTCTGGA ACTCCACAGG GAGAAGCCTC TACACCCGAG 
    GAAATGCGTG AAGAGAAAAA GGAGCAGGAA GGAGACAAGG GAGCTTCTTC GGAGGAGAGC 
    CGAGCTGCTA AGGCTAAAGC TAGCAAACCT CTGATGCCCA CCTCTACCAT CTTGAGGCTG 
    TTGGCCGAGC TGGTTCGCTC TTATGTTGGC ATTGCCACTC TTATCACCTC TTACAACTAC 
    ACAGCTGGCC AGTCGGAGCT CATTAAGGAA GATTGCAGTG TGTTGGCATT TGTTTTGGAC 
    CACTTGCTCC CACACACCCA GAACTCTGAA GATAAGGACA CCCCTGCCCT TGCACGCCTT 
    TTCTTGGCCA GCCTTGCTGC AGCTGGCACG GGCACCGATG CCCAGGCTGC ACTGGTCAAC 
    GAGGTCAAAG CTGCTTTGAG TCGAGCATTG GGCATGGCAG AGGGCACAGA GAAACACGCC 
    CGTTTGCAAG CAGTGATGTG CATCATCAGC ACCATTATGG AGTCGTGTCC TTCCACCTCC 
    AGCTTCTACA GCACAGCCAC AGCTAAGACC CAGCACAACG GCATGAACAA TATCATCCGG 
    CTCTTCTTGA AGAAAGGCCT TGTCAATGAC CTGGCCCGTG TGCCTCACAG CCTTGATCTA 
    TCCAGTCCCA ATATGGCAAA CACAGTAAAT GCTGCTTTGA AGCCTCTGGA AACATTGTCG 
    CGAATTGTGA ACCAGCCCAG CAGCCTGTTT GGTGGTAAAG GGGGATCCAG CAAGAACAAG 
    ACTGAACATG ACACTGTTGG CACAGTCAGA GACAGTAACA GCAACACTCA GGATCAAGGA 
    GAAGTGGGTG AGGCAGAGCC GGTTGAGAAT CGTGACCGAG GTCAAGCCAC AGACTCTGAC 
    CTGATGGATG GAGAAGCCGA GGGAGACACT GTGGTCATTG CTGGACAGCC TGAGGTGCTG 
    AGCACTCAGG CAATGCAGGT GGAGAACGAG TTGGTGGATT TGATCGATGA ACTTTTAGAG 
    AGAGATGCCC AAACTGTTAA TAGTACCATC ATCGTAGGTC GTTCTGGAGA GGATGAGTCT 
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA 
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG 
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG 
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC 
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT 
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA 
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC 
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA 
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT 
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC 
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT 
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG 
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA 
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC 
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT 
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG 
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG 
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC 
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG 
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT 
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC 
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT 
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT 
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA 
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT 
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT 
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT 
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT 
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG 
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA 
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT 
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA 
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG 
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG 
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC 
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA 
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC 
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC 
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG 
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT 
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG 
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC 
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG 
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA 
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG 
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG 
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT 
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG 
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA 
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG 
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC 
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT 
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT 
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT 
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC 
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC 
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT 
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG 
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT 
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG 
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG 
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC 
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA 
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT 
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA 
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT 
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA 
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA 
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG 
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG 
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC 
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA 
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG 
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG 
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT 
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT 
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG 
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA 
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG 
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG 
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT 
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC 
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT 
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC 
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG 
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT 
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG 
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC 
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG 
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC 
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC 
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC 
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT 
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG 
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG 
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG 
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT 
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC 
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG 
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT 
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA 
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG 
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC 
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC 
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA 
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329382.1
    CDS142..13848
    Translation

    Target ORF information:

    RefSeq Version XM_021473707.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021473707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGAGAGAGG AGAGGAGCGG GCAAACGCTG TACCAGGGAA TCAAGACCTG 
    TGTCAGATCC TGCAGGATGT CGGTGATGAT GTCTACCAGC AGTACAGACT CACCAGACAA 
    GGCAGTGATT TTGACTCGCA ATCTGCCTTC CCCATCAATA CTCAGGTGTT TGCTGCAGAT 
    GGTGCTGTAG CTGAATCTTC TCAGTCTGGA ACTCCACAGG GAGAAGCCTC TACACCCGAG 
    GAAATGCGTG AAGAGAAAAA GGAGCAGGAA GGAGACAAGG GAGCTTCTTC GGAGGAGAGC 
    CGAGCTGCTA AGGCTAAAGC TAGCAAACCT CTGATGCCCA CCTCTACCAT CTTGAGGCTG 
    TTGGCCGAGC TGGTTCGCTC TTATGTTGGC ATTGCCACTC TTATCACCTC TTACAACTAC 
    ACAGCTGGCC AGTCGGAGCT CATTAAGGAA GATTGCAGTG TGTTGGCATT TGTTTTGGAC 
    CACTTGCTCC CACACACCCA GAACTCTGAA GATAAGGACA CCCCTGCCCT TGCACGCCTT 
    TTCTTGGCCA GCCTTGCTGC AGCTGGCACG GGCACCGATG CCCAGGCTGC ACTGGTCAAC 
    GAGGTCAAAG CTGCTTTGAG TCGAGCATTG GGCATGGCAG AGGGCACAGA GAAACACGCC 
    CGTTTGCAAG CAGTGATGTG CATCATCAGC ACCATTATGG AGTCGTGTCC TTCCACCTCC 
    AGCTTCTACA GCACAGCCAC AGCTAAGACC CAGCACAACG GCATGAACAA TATCATCCGG 
    CTCTTCTTGA AGAAAGGCCT TGTCAATGAC CTGGCCCGTG TGCCTCACAG CCTTGATCTA 
    TCCAGTCCCA ATATGGCAAA CACAGTAAAT GCTGCTTTGA AGCCTCTGGA AACATTGTCG 
    CGAATTGTGA ACCAGCCCAG CAGCCTGTTT GGTGGTAAAG GGGGATCCAG CAAGAACAAG 
    ACTGAACATG ACACTGTTGG CACAGTCAGA GACAGTAACA GCAACACTCA GGATCAAGGA 
    GAAGTGGGTG AGGCAGAGCC GGTTGAGAAT CGTGACCGAG GTCAAGCCAC AGACTCTGAC 
    CTGATGGATG GAGAAGCCGA GGGAGACACT GTGGTCATTG CTGGACAGCC TGAGGTGCTG 
    AGCACTCAGG CAATGCAGGT GGAGAACGAG TTGGTGGATT TGATCGATGA ACTTTTAGAG 
    AGAGATGCCC AAACTGTTAA TAGTACCATC ATCGTAGGTC GTTCTGGAGA GGATGAGTCT 
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA 
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG 
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG 
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC 
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT 
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA 
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC 
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA 
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT 
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC 
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT 
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG 
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA 
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC 
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT 
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG 
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG 
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC 
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG 
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT 
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC 
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT 
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT 
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA 
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT 
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT 
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT 
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT 
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG 
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA 
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT 
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA 
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG 
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG 
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC 
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA 
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC 
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC 
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG 
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT 
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG 
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC 
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG 
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA 
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG 
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG 
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT 
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG 
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA 
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG 
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC 
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT 
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT 
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT 
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC 
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC 
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT 
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG 
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT 
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG 
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG 
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC 
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA 
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT 
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA 
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT 
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA 
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA 
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG 
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG 
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC 
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA 
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG 
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG 
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT 
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT 
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG 
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA 
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG 
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG 
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT 
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC 
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT 
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC 
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG 
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT 
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG 
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC 
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG 
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC 
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC 
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC 
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT 
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG 
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG 
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG 
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT 
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC 
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG 
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT 
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA 
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG 
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC 
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC 
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA 
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_005162280.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162337.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa27856
    Clone ID Related Accession (Same CDS sequence) XM_021473707.1 , XM_005162280.3 , XM_005162280.4
    Accession Version XM_005162280.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162280.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGAGAGAGG AGAGGAGCGG GCAAACGCTG TACCAGGGAA TCAAGACCTG 
    TGTCAGATCC TGCAGGATGT CGGTGATGAT GTCTACCAGC AGTACAGACT CACCAGACAA 
    GGCAGTGATT TTGACTCGCA ATCTGCCTTC CCCATCAATA CTCAGGTGTT TGCTGCAGAT 
    GGTGCTGTAG CTGAATCTTC TCAGTCTGGA ACTCCACAGG GAGAAGCCTC TACACCCGAG 
    GAAATGCGTG AAGAGAAAAA GGAGCAGGAA GGAGACAAGG GAGCTTCTTC GGAGGAGAGC 
    CGAGCTGCTA AGGCTAAAGC TAGCAAACCT CTGATGCCCA CCTCTACCAT CTTGAGGCTG 
    TTGGCCGAGC TGGTTCGCTC TTATGTTGGC ATTGCCACTC TTATCACCTC TTACAACTAC 
    ACAGCTGGCC AGTCGGAGCT CATTAAGGAA GATTGCAGTG TGTTGGCATT TGTTTTGGAC 
    CACTTGCTCC CACACACCCA GAACTCTGAA GATAAGGACA CCCCTGCCCT TGCACGCCTT 
    TTCTTGGCCA GCCTTGCTGC AGCTGGCACG GGCACCGATG CCCAGGCTGC ACTGGTCAAC 
    GAGGTCAAAG CTGCTTTGAG TCGAGCATTG GGCATGGCAG AGGGCACAGA GAAACACGCC 
    CGTTTGCAAG CAGTGATGTG CATCATCAGC ACCATTATGG AGTCGTGTCC TTCCACCTCC 
    AGCTTCTACA GCACAGCCAC AGCTAAGACC CAGCACAACG GCATGAACAA TATCATCCGG 
    CTCTTCTTGA AGAAAGGCCT TGTCAATGAC CTGGCCCGTG TGCCTCACAG CCTTGATCTA 
    TCCAGTCCCA ATATGGCAAA CACAGTAAAT GCTGCTTTGA AGCCTCTGGA AACATTGTCG 
    CGAATTGTGA ACCAGCCCAG CAGCCTGTTT GGTGGTAAAG GGGGATCCAG CAAGAACAAG 
    ACTGAACATG ACACTGTTGG CACAGTCAGA GACAGTAACA GCAACACTCA GGATCAAGGA 
    GAAGTGGGTG AGGCAGAGCC GGTTGAGAAT CGTGACCGAG GTCAAGCCAC AGACTCTGAC 
    CTGATGGATG GAGAAGCCGA GGGAGACACT GTGGTCATTG CTGGACAGCC TGAGGTGCTG 
    AGCACTCAGG CAATGCAGGT GGAGAACGAG TTGGTGGATT TGATCGATGA ACTTTTAGAG 
    AGAGATGCCC AAACTGTTAA TAGTACCATC ATCGTAGGTC GTTCTGGAGA GGATGAGTCT 
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA 
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG 
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG 
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC 
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT 
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA 
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC 
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA 
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT 
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC 
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT 
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG 
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA 
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC 
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT 
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG 
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG 
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC 
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG 
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT 
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC 
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT 
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT 
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA 
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT 
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT 
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT 
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT 
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG 
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA 
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT 
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA 
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG 
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG 
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC 
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA 
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC 
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC 
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG 
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT 
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG 
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC 
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG 
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA 
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG 
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG 
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT 
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG 
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA 
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG 
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC 
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT 
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT 
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT 
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC 
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC 
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT 
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG 
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT 
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG 
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG 
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC 
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA 
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT 
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA 
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT 
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA 
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA 
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG 
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG 
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC 
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA 
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG 
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG 
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT 
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT 
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG 
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA 
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG 
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG 
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT 
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC 
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT 
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC 
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG 
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT 
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG 
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC 
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG 
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC 
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC 
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC 
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT 
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG 
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG 
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG 
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT 
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC 
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG 
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT 
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA 
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG 
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC 
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC 
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA 
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162337.1
    CDS149..13855
    Translation

    Target ORF information:

    RefSeq Version XM_005162280.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005162280.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGAGAGAGG AGAGGAGCGG GCAAACGCTG TACCAGGGAA TCAAGACCTG 
    TGTCAGATCC TGCAGGATGT CGGTGATGAT GTCTACCAGC AGTACAGACT CACCAGACAA 
    GGCAGTGATT TTGACTCGCA ATCTGCCTTC CCCATCAATA CTCAGGTGTT TGCTGCAGAT 
    GGTGCTGTAG CTGAATCTTC TCAGTCTGGA ACTCCACAGG GAGAAGCCTC TACACCCGAG 
    GAAATGCGTG AAGAGAAAAA GGAGCAGGAA GGAGACAAGG GAGCTTCTTC GGAGGAGAGC 
    CGAGCTGCTA AGGCTAAAGC TAGCAAACCT CTGATGCCCA CCTCTACCAT CTTGAGGCTG 
    TTGGCCGAGC TGGTTCGCTC TTATGTTGGC ATTGCCACTC TTATCACCTC TTACAACTAC 
    ACAGCTGGCC AGTCGGAGCT CATTAAGGAA GATTGCAGTG TGTTGGCATT TGTTTTGGAC 
    CACTTGCTCC CACACACCCA GAACTCTGAA GATAAGGACA CCCCTGCCCT TGCACGCCTT 
    TTCTTGGCCA GCCTTGCTGC AGCTGGCACG GGCACCGATG CCCAGGCTGC ACTGGTCAAC 
    GAGGTCAAAG CTGCTTTGAG TCGAGCATTG GGCATGGCAG AGGGCACAGA GAAACACGCC 
    CGTTTGCAAG CAGTGATGTG CATCATCAGC ACCATTATGG AGTCGTGTCC TTCCACCTCC 
    AGCTTCTACA GCACAGCCAC AGCTAAGACC CAGCACAACG GCATGAACAA TATCATCCGG 
    CTCTTCTTGA AGAAAGGCCT TGTCAATGAC CTGGCCCGTG TGCCTCACAG CCTTGATCTA 
    TCCAGTCCCA ATATGGCAAA CACAGTAAAT GCTGCTTTGA AGCCTCTGGA AACATTGTCG 
    CGAATTGTGA ACCAGCCCAG CAGCCTGTTT GGTGGTAAAG GGGGATCCAG CAAGAACAAG 
    ACTGAACATG ACACTGTTGG CACAGTCAGA GACAGTAACA GCAACACTCA GGATCAAGGA 
    GAAGTGGGTG AGGCAGAGCC GGTTGAGAAT CGTGACCGAG GTCAAGCCAC AGACTCTGAC 
    CTGATGGATG GAGAAGCCGA GGGAGACACT GTGGTCATTG CTGGACAGCC TGAGGTGCTG 
    AGCACTCAGG CAATGCAGGT GGAGAACGAG TTGGTGGATT TGATCGATGA ACTTTTAGAG 
    AGAGATGCCC AAACTGTTAA TAGTACCATC ATCGTAGGTC GTTCTGGAGA GGATGAGTCT 
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA 
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG 
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG 
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC 
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT 
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA 
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC 
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA 
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT 
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC 
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT 
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG 
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA 
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC 
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT 
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG 
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG 
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC 
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG 
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT 
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC 
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT 
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT 
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA 
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT 
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT 
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT 
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT 
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG 
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA 
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT 
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA 
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG 
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG 
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC 
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA 
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC 
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC 
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG 
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT 
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG 
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC 
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG 
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA 
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG 
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG 
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT 
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG 
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA 
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG 
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC 
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT 
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT 
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT 
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC 
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC 
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT 
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG 
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT 
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG 
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG 
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC 
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA 
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT 
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA 
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT 
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA 
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA 
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG 
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG 
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC 
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA 
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG 
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG 
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT 
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT 
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG 
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA 
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG 
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG 
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT 
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC 
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT 
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC 
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG 
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT 
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG 
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC 
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG 
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC 
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC 
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC 
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT 
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG 
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG 
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG 
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT 
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC 
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG 
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT 
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA 
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG 
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC 
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC 
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA 
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_005162280.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162337.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa27856
    Clone ID Related Accession (Same CDS sequence) XM_021473707.1 , XM_005162280.3 , XM_005162280.4
    Accession Version XM_005162280.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162280.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGAGAGAGG AGAGGAGCGG GCAAACGCTG TACCAGGGAA TCAAGACCTG 
    TGTCAGATCC TGCAGGATGT CGGTGATGAT GTCTACCAGC AGTACAGACT CACCAGACAA 
    GGCAGTGATT TTGACTCGCA ATCTGCCTTC CCCATCAATA CTCAGGTGTT TGCTGCAGAT 
    GGTGCTGTAG CTGAATCTTC TCAGTCTGGA ACTCCACAGG GAGAAGCCTC TACACCCGAG 
    GAAATGCGTG AAGAGAAAAA GGAGCAGGAA GGAGACAAGG GAGCTTCTTC GGAGGAGAGC 
    CGAGCTGCTA AGGCTAAAGC TAGCAAACCT CTGATGCCCA CCTCTACCAT CTTGAGGCTG 
    TTGGCCGAGC TGGTTCGCTC TTATGTTGGC ATTGCCACTC TTATCACCTC TTACAACTAC 
    ACAGCTGGCC AGTCGGAGCT CATTAAGGAA GATTGCAGTG TGTTGGCATT TGTTTTGGAC 
    CACTTGCTCC CACACACCCA GAACTCTGAA GATAAGGACA CCCCTGCCCT TGCACGCCTT 
    TTCTTGGCCA GCCTTGCTGC AGCTGGCACG GGCACCGATG CCCAGGCTGC ACTGGTCAAC 
    GAGGTCAAAG CTGCTTTGAG TCGAGCATTG GGCATGGCAG AGGGCACAGA GAAACACGCC 
    CGTTTGCAAG CAGTGATGTG CATCATCAGC ACCATTATGG AGTCGTGTCC TTCCACCTCC 
    AGCTTCTACA GCACAGCCAC AGCTAAGACC CAGCACAACG GCATGAACAA TATCATCCGG 
    CTCTTCTTGA AGAAAGGCCT TGTCAATGAC CTGGCCCGTG TGCCTCACAG CCTTGATCTA 
    TCCAGTCCCA ATATGGCAAA CACAGTAAAT GCTGCTTTGA AGCCTCTGGA AACATTGTCG 
    CGAATTGTGA ACCAGCCCAG CAGCCTGTTT GGTGGTAAAG GGGGATCCAG CAAGAACAAG 
    ACTGAACATG ACACTGTTGG CACAGTCAGA GACAGTAACA GCAACACTCA GGATCAAGGA 
    GAAGTGGGTG AGGCAGAGCC GGTTGAGAAT CGTGACCGAG GTCAAGCCAC AGACTCTGAC 
    CTGATGGATG GAGAAGCCGA GGGAGACACT GTGGTCATTG CTGGACAGCC TGAGGTGCTG 
    AGCACTCAGG CAATGCAGGT GGAGAACGAG TTGGTGGATT TGATCGATGA ACTTTTAGAG 
    AGAGATGCCC AAACTGTTAA TAGTACCATC ATCGTAGGTC GTTCTGGAGA GGATGAGTCT 
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA 
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG 
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG 
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC 
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT 
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA 
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC 
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA 
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT 
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC 
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT 
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG 
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA 
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC 
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT 
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG 
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG 
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC 
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG 
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT 
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC 
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT 
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT 
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA 
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT 
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT 
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT 
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT 
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG 
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA 
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT 
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA 
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG 
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG 
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC 
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA 
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC 
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC 
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG 
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT 
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG 
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC 
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG 
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA 
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG 
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG 
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT 
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG 
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA 
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG 
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC 
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT 
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT 
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT 
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC 
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC 
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT 
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG 
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT 
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG 
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG 
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC 
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA 
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT 
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA 
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT 
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA 
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA 
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG 
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG 
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC 
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA 
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG 
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG 
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT 
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT 
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG 
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA 
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG 
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG 
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT 
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC 
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT 
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC 
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG 
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT 
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG 
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC 
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG 
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC 
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC 
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC 
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT 
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG 
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG 
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG 
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT 
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC 
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG 
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT 
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA 
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG 
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC 
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC 
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA 
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162337.1
    CDS142..13848
    Translation

    Target ORF information:

    RefSeq Version XM_005162280.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005162280.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGAGAGAGG AGAGGAGCGG GCAAACGCTG TACCAGGGAA TCAAGACCTG 
    TGTCAGATCC TGCAGGATGT CGGTGATGAT GTCTACCAGC AGTACAGACT CACCAGACAA 
    GGCAGTGATT TTGACTCGCA ATCTGCCTTC CCCATCAATA CTCAGGTGTT TGCTGCAGAT 
    GGTGCTGTAG CTGAATCTTC TCAGTCTGGA ACTCCACAGG GAGAAGCCTC TACACCCGAG 
    GAAATGCGTG AAGAGAAAAA GGAGCAGGAA GGAGACAAGG GAGCTTCTTC GGAGGAGAGC 
    CGAGCTGCTA AGGCTAAAGC TAGCAAACCT CTGATGCCCA CCTCTACCAT CTTGAGGCTG 
    TTGGCCGAGC TGGTTCGCTC TTATGTTGGC ATTGCCACTC TTATCACCTC TTACAACTAC 
    ACAGCTGGCC AGTCGGAGCT CATTAAGGAA GATTGCAGTG TGTTGGCATT TGTTTTGGAC 
    CACTTGCTCC CACACACCCA GAACTCTGAA GATAAGGACA CCCCTGCCCT TGCACGCCTT 
    TTCTTGGCCA GCCTTGCTGC AGCTGGCACG GGCACCGATG CCCAGGCTGC ACTGGTCAAC 
    GAGGTCAAAG CTGCTTTGAG TCGAGCATTG GGCATGGCAG AGGGCACAGA GAAACACGCC 
    CGTTTGCAAG CAGTGATGTG CATCATCAGC ACCATTATGG AGTCGTGTCC TTCCACCTCC 
    AGCTTCTACA GCACAGCCAC AGCTAAGACC CAGCACAACG GCATGAACAA TATCATCCGG 
    CTCTTCTTGA AGAAAGGCCT TGTCAATGAC CTGGCCCGTG TGCCTCACAG CCTTGATCTA 
    TCCAGTCCCA ATATGGCAAA CACAGTAAAT GCTGCTTTGA AGCCTCTGGA AACATTGTCG 
    CGAATTGTGA ACCAGCCCAG CAGCCTGTTT GGTGGTAAAG GGGGATCCAG CAAGAACAAG 
    ACTGAACATG ACACTGTTGG CACAGTCAGA GACAGTAACA GCAACACTCA GGATCAAGGA 
    GAAGTGGGTG AGGCAGAGCC GGTTGAGAAT CGTGACCGAG GTCAAGCCAC AGACTCTGAC 
    CTGATGGATG GAGAAGCCGA GGGAGACACT GTGGTCATTG CTGGACAGCC TGAGGTGCTG 
    AGCACTCAGG CAATGCAGGT GGAGAACGAG TTGGTGGATT TGATCGATGA ACTTTTAGAG 
    AGAGATGCCC AAACTGTTAA TAGTACCATC ATCGTAGGTC GTTCTGGAGA GGATGAGTCT 
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA 
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG 
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG 
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC 
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT 
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA 
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC 
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA 
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT 
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC 
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT 
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG 
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA 
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC 
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT 
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG 
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG 
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC 
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG 
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT 
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC 
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT 
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT 
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA 
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT 
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT 
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT 
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT 
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG 
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA 
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT 
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA 
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG 
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG 
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC 
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA 
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC 
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC 
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG 
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT 
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG 
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC 
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG 
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA 
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG 
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG 
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT 
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG 
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA 
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG 
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC 
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT 
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT 
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT 
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC 
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC 
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT 
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG 
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT 
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG 
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG 
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC 
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA 
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT 
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA 
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT 
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA 
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA 
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG 
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG 
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC 
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA 
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG 
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG 
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT 
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT 
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG 
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA 
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG 
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG 
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT 
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC 
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT 
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC 
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG 
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT 
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG 
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC 
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG 
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC 
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC 
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC 
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT 
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG 
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG 
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG 
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT 
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC 
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG 
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT 
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA 
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG 
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC 
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC 
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA 
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_021473708.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329383.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa27857
    Clone ID Related Accession (Same CDS sequence) XM_021473708.1 , XM_005162281.3 , XM_005162281.4
    Accession Version XM_021473708.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    1021
    1081
    1141
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    1261
    1321
    1381
    1441
    1501
    1561
    1621
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    1801
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    1921
    1981
    2041
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    2641
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    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGGTC GTTCTGGAGA GGATGAGTCT 
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA 
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG 
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG 
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC 
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT 
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA 
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC 
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA 
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT 
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC 
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT 
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG 
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA 
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC 
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT 
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG 
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG 
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC 
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG 
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT 
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC 
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT 
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT 
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA 
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT 
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT 
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT 
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT 
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG 
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA 
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT 
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA 
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG 
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG 
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC 
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA 
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC 
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC 
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG 
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT 
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG 
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC 
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG 
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA 
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG 
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG 
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT 
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG 
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA 
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG 
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC 
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT 
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT 
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT 
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC 
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC 
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT 
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG 
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT 
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG 
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG 
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC 
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA 
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT 
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA 
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT 
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA 
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA 
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG 
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG 
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC 
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA 
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG 
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG 
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT 
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT 
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG 
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA 
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG 
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG 
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT 
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC 
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT 
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC 
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG 
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT 
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG 
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC 
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG 
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC 
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC 
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC 
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT 
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG 
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG 
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG 
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT 
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC 
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG 
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT 
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA 
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG 
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC 
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC 
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA 
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329383.1
    CDS142..13848
    Translation

    Target ORF information:

    RefSeq Version XM_021473708.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021473708.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGGTC GTTCTGGAGA GGATGAGTCT 
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA 
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG 
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG 
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC 
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT 
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA 
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC 
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA 
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT 
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC 
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT 
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG 
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA 
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC 
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT 
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG 
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG 
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC 
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG 
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT 
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC 
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT 
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT 
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA 
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT 
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT 
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT 
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT 
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG 
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA 
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT 
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA 
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG 
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG 
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC 
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA 
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC 
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC 
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG 
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT 
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG 
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC 
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG 
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA 
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG 
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG 
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT 
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG 
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA 
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG 
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC 
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT 
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT 
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT 
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC 
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC 
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT 
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG 
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT 
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG 
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG 
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC 
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA 
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT 
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA 
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT 
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA 
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA 
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG 
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG 
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC 
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA 
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG 
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG 
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT 
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT 
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG 
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA 
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG 
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG 
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT 
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC 
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT 
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC 
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG 
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT 
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG 
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC 
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG 
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC 
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC 
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC 
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT 
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG 
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG 
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG 
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT 
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC 
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG 
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT 
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA 
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG 
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC 
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC 
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA 
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_005162281.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162338.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa27857
    Clone ID Related Accession (Same CDS sequence) XM_021473708.1 , XM_005162281.3 , XM_005162281.4
    Accession Version XM_005162281.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162281.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGGTC GTTCTGGAGA GGATGAGTCT 
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA 
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG 
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG 
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC 
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT 
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA 
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC 
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA 
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT 
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC 
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT 
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG 
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA 
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC 
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT 
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG 
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG 
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC 
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG 
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT 
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC 
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT 
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT 
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA 
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT 
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT 
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT 
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT 
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG 
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA 
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT 
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA 
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG 
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG 
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC 
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA 
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC 
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC 
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG 
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT 
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG 
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC 
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG 
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA 
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG 
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG 
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT 
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG 
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA 
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG 
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC 
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT 
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT 
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT 
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC 
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC 
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT 
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG 
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT 
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG 
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG 
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC 
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA 
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT 
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA 
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT 
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA 
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA 
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG 
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG 
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC 
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA 
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG 
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG 
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT 
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT 
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG 
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA 
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG 
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG 
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT 
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC 
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT 
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC 
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG 
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT 
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG 
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC 
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG 
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC 
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC 
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC 
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT 
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG 
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG 
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG 
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT 
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC 
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG 
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT 
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA 
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG 
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC 
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC 
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA 
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162338.1
    CDS149..13855
    Translation

    Target ORF information:

    RefSeq Version XM_005162281.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005162281.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGGTC GTTCTGGAGA GGATGAGTCT 
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA 
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG 
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG 
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC 
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT 
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA 
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC 
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA 
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT 
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC 
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT 
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG 
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA 
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC 
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT 
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG 
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG 
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC 
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG 
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT 
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC 
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT 
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT 
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA 
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT 
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT 
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT 
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT 
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG 
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA 
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT 
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA 
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG 
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG 
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC 
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA 
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC 
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC 
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG 
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT 
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG 
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC 
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG 
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA 
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG 
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG 
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT 
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG 
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA 
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG 
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC 
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT 
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT 
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT 
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC 
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC 
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT 
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG 
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT 
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG 
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG 
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC 
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA 
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT 
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA 
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT 
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA 
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA 
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG 
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG 
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC 
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA 
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG 
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG 
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT 
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT 
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG 
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA 
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG 
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG 
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT 
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC 
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT 
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC 
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG 
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT 
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG 
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC 
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG 
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC 
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC 
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC 
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT 
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG 
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG 
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG 
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT 
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC 
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG 
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT 
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA 
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG 
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC 
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC 
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA 
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_005162281.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162338.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa27857
    Clone ID Related Accession (Same CDS sequence) XM_021473708.1 , XM_005162281.3 , XM_005162281.4
    Accession Version XM_005162281.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162281.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGGTC GTTCTGGAGA GGATGAGTCT 
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA 
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG 
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG 
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC 
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT 
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA 
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC 
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA 
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT 
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC 
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT 
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG 
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA 
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC 
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT 
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG 
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG 
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC 
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG 
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT 
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC 
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT 
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT 
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA 
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT 
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT 
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT 
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT 
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG 
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA 
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT 
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA 
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG 
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG 
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC 
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA 
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC 
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC 
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG 
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT 
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG 
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC 
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG 
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA 
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG 
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG 
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT 
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG 
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA 
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG 
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC 
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT 
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT 
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT 
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC 
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC 
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT 
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG 
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT 
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG 
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG 
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC 
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA 
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT 
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA 
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT 
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA 
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA 
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG 
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG 
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC 
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA 
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG 
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG 
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT 
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT 
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG 
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA 
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG 
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG 
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT 
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC 
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT 
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC 
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG 
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT 
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG 
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC 
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG 
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC 
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC 
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC 
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT 
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG 
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG 
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG 
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT 
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC 
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG 
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT 
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA 
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG 
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC 
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC 
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA 
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162338.1
    CDS142..13848
    Translation

    Target ORF information:

    RefSeq Version XM_005162281.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005162281.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    13681
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGGTC GTTCTGGAGA GGATGAGTCT 
    CAAGAGGATG TGCTGATGGA TGAAGCTCCT TCCAATATGA GTCAGGCCTC CACTCTCCAA 
    GCCAACAGAG AAGACTCCAT GAACATCCTC GAGCCAGAAG ATGAGGAGCA CACGCAGGAG 
    GAAGACTCCA GTGGTAGCAA CGAGGATGAG GACAGTCAGG ATGAAGAGGA AGAGGAGGAG 
    GAAGAAGAGG AGGAAGATCA GGATGATGAA GAGGGAGATG AGGATGATGA CGATGAAGGC 
    TCAGAGATGG AACTGGATGA AGATTTTCCA GATATCAATG CTGCCCCTCA TATTCGTTTT 
    GAGCGATTCG ACCGAGATGA TGACCTCATC ATTGAGTTTG ATAATATGTT CTCCACTTCA 
    GCTGACATCC CTCCCTCACC AGGAAATATC CCCAGCTCCC ACCCTCTGAT GGTGCGTCAC 
    GCTGACCATG GCTCGCTGAC TCTTGGGGGA TCGGGCACCA GTAGCAGATT GGCTCAAGGA 
    ATGGGACGTA GTCAACGTTC TCTCCGCCAG CTCACGGCCA ACACAGGCCA CACGATCCAT 
    GTACATTATC CTGGCAACCG TCAGCCTAAC CCTCCACTCA TTCTGCAGAG ATTATTGGGC 
    CCCAGCGCTG CAGCTGATAT CTTGCAGCTG TCCAGCTCTC TTCCTCTGCA GTCTCGAGGT 
    CGTGCCCGTT TAATGGTGGG TAATGAAGAT GTGCACATTA TTGCCCGTTC AGACGATGAG 
    CTGCTGGATG ACTTCTTCCA TGAGCAGAGC AGCACCGGAG GACAAGCTGG CACCCTTTCA 
    AGTATCCCGA CTGCCTTAAC CAGATGGACA GATGAGTGTA AAGTGCTGGA TGCTGAGAGC 
    ATGCATGACT GTGTGGCAGT GGTGAAGGTG CCCATACTGC AGCACCTGGA GAGTTTGCGT 
    GATGAGGAGC TGGAGGAACG CAGGGAGAAA AGGAGGAGGC AGTTGGCAGA GGAAGAGGAG 
    ACTGCAAAGC AGAATGAGAG TTCTGCAGCA GGGGAGGAGA CCAGGGAACA GAGTCTGCAG 
    GGCTCTGGGA TTGGAACAGT CAATGGAGCT GCAGAGAATG CTGCAGAAGG AGAACAGGCC 
    CAGAGTGGGG TGTCCTCCTG TCTGGACCCT CCTCGCACCT CTGAGGGTTT CCTCACAGCG 
    CCCCCATCAG GAGAAGTCAC TCCAACCACC CCTGCCCCCC ATGAACAAGC CCTCGTGTCT 
    CTGGAAACAG CCATCAGTCA GCAGGTGCAC CAGCCAATCA CAGAGCTGCT GCTGGCCGAC 
    TCCAATCCTG ACTCTCACGG AGCCTTGGCA GGGGCAGGGC TTCCCCAGCT CTCAGCACCT 
    GATAGGCCAA ATTGTGAGGC AGAAGCATCT CAGATGGAGA TGTCACCTGC ACCCACTATT 
    GGTGAGAGAG GAGGAGGAGC ATTGGATGCA GTCAGGGAAG CTTCACTCAG TCCAGATATA 
    GCTGAAAGTT CAGAGCCTGT GCCAGTGGGT GTGTCTCAAC TCGAGGGTTC GCCCATGGAT 
    ACGAGCAGTC CCGCTTCAGG AACGCAGGAA GAGCCGGCTG CCAGCTCAGT TCCATCCAAT 
    CACATGTCTC AGGAGCTATC AGGAAGTGGT GACAGCGGCC TCACAGACAG GCAGACAGAT 
    GCAGAAACTG GATCAACTTC TGTTTCATCT CCTGGCGAGA CCATGCCTCG GAGTGACAGT 
    GCCGATAGCC AGTCGCAGGC CATCCAGGAG GAGCCCCTCC CTTCCACCAG CAATGAAGAG 
    GAGGACCCTC TGGCAGGCAT CAGTCTACCT GAAGGCGTGG ATCCGTCTTT CTTGGCAGCA 
    CTGCCTGAAG ACATTCGTCG GGAGGTGCTG CAGAACCAAC TGGGAATCAG ACCTCCTTCT 
    CGACCTCCTG TCGCCTCCAC TCTGACAACC ACTGCTAGTG CTGTGCTAGG AGGAGGGCCA 
    GGGGTGACCG AAGTCAGCCC TGAATTCTTA GCGGCTCTCC CACCTGCAAT TCAGGAAGAG 
    GTCCTGGCCC AGCAGCGGGC AGAGCAGCAG CGTCGGGAGT TATCCCAGCA ACCCACACAG 
    GGTGATCAAC CTCTGGACCC AGTCACATTC ATCCAGACCC TGCCCTCTGA GCTGCGGCGC 
    TCCGTTCTGG AAGACATGGA GGACAGTGTA CTAGCGGTCA TGCCTCCAGA TATTGCAGCA 
    GAGGCTGCAG CACTGCGAAG GGAACAAGAG GCCAGACAGA GACAACTGAT GCACGAGCGC 
    TTGTTCGGTC ACAGCTCCTC TTCAGCTCTG TCTGCCATCT TACGCTCTCC CGCCTTCACC 
    AGCCGCCTCG GGGGCAACCG TGGGGTACAG TACACCCGGC TGGCTGTGCA AAGAGGAGGG 
    ACGTTTCAAA TGGGAGGGAG TGCCAATCAT AGCAGACCCT CTAGTTCAAA CGTTGACTCT 
    CTCCTGCGCC TGCGGGGGCG TCTCTTGTTG GACCACGAGG CCTTGTCCTG CCTTCTTGTG 
    CTGCTTTTTG TGGACGAGCC CAAACTTAAC ACCAGCCGCC TCCACCGAGT GCTGCGTAAC 
    TTGTGCTATC ACTCTCAGAC GCGTGGCTGG GTCATCCGCA GTCTCCTGTC CATCCTGCAG 
    CGCAGCAGTG AGAGCGAGCT GTGCCTGGAG ACTGCACGCT TGGAGGAGTC CCGTGGCAAA 
    CGCTCACTAG GCCAGGGCTC ATCTAGTGGA GGAAAGGGTT GTTCTTCATC CTCCCAGTCG 
    GCTATAGCCT CTTCGTCATC GTCCTCCTCG TCCTCTTCTT CTCTGGAGCT GCTGAACAGG 
    GTGGAGTCAC GGAGTTCCAG TCAGCTCTCC TGGCTGTCTG TATCAATGGA CGCAGCTCTT 
    GGATGCAGAA CCAACATCTT TCAGATCCAG CGAGTATCAG GCAGGAAACA CGCAGAGAGG 
    CATTCAGCAG GAGGGAGTGT GAGCGCAGGA GGACTCACAG GTGGAGTGTC AGGAACAGGA 
    ATGACGTGTA CAGGGGGAGG TGGGTCAACT GTTCACATCC ATCCACAGGC TGCGCCGGTG 
    GTCTGTCGAC ATGTACTGGA CACCCTCATC CAATTGGCCA AGGTATTTCC AAGTCACTTC 
    ACTCAACAAC GCTGTAAAGA TCCCCTATCC TCCTCCTCCT CTGAGTTGGA CACTCGTCTT 
    TGTGCCAGCT CAACACCGAC TATGAGTGCC AGTTGTCGGT CAGGTGGCAC CAGTCAGAGT 
    AACTCCTCTG CTACCCCAAG TAGTCTCACT TCTATCCACA ATTCCCTGAG CGCCTGCTCT 
    GGTACGCCAC AGTCCCTAGG CATCTCCACC GACTTTTGGG ATCTCCTGGT CAAGCTGGAC 
    AACATGAATG TGAGCCGAAA GGGGAAGGCC TCCATGAAGA CTGTCCCTCT AGGGGGTGTC 
    GGCGAGACAG AGGGTGCGCT GTTCAGCCTT GAGGCGTCTC CGCTGGGCCA GCTGATGAAT 
    ATGCTGTCCC ACCCTGTGAT CCGCCGTAGT TCCCTCCTCA CGGAGAAACT GCTACGTCTG 
    CTGTCTCTGA TCTCCATCGC TCTGCCTGAC AACAAGGCCA CGGAGGTTCC AGCCAGCCAT 
    CCAGTGCCTC AGACCCCAAC TGCCACGCCA TCTGCACCTG CTCCTCAAAC CACAGCAGGG 
    TCAGGGACCA TCTCAGCAGT GGCAGCTACA GGCACTACGC AGGGTTCAGT CGGTTCAGTG 
    GGTACAACGC CATCTCAACC AGCCGCTACA ATATCTGTGG TTGCAGGCAG CACCACCGCC 
    TCTAGCATTA TTGCTGGAAA ACTGAAGCCA GCAGTAAGCA TCACGGACAT TGAATCTAAA 
    CTGGCCTCTA CTGGACTTAC GGAGAAGCAG CTGCAGCTCT CTGTGGAGGT TCTGACATCT 
    CACTCGTGTT CAGAGGAAGG TCTGGAGGAC GCAGCCAACA TCTTGCTGCA GCTGTCCAGA 
    GGGGACGCAC CGACCCGAGA CACTGTGCTT AGACTTCTGC TCAGCGGAGC ACGTCACCTT 
    GGATACACTC TCTGCAAACA AATCGGCACT TTATTAGCTG AGTTGAGGGA GTATAATCTA 
    GAGCAGCAGA GAAGAGCACG GGCTGATGCT CAGTCTCCTG AAGCTTCTCA TGATGACCCA 
    TCCATCACCA CACGCCTCAA GGGCAAGATG ACCAGCAGGT TTGACGGGTC AGAGAGTGTG 
    GTGATTGTTG CTGCTCAGAA GCGCACGTTA GGTGGCCGGG AGCTTCAGTT GCCCTGCATG 
    AGCTCGCTCA CTTCCAAGAC CTCAACGCAG AAGTTTTTCT TGCGGGTACT GCAGGTCATC 
    ATCCAGCTTA GAGAAGACAC GCGGCGCGCT AACAAGAAAG CCAAGCAGAC AGGAGGAAGA 
    CTAAGTTCAA CCAGTCTGGG CTCGGCCAGT AGTATTCAGG CTGCGGTCCG TCAGCTGGAG 
    GCTGAAGCGG ACGCCATCAT TCAGATGGTG AGAGAGGGAC AGAGGGCACG CCGTCTACAG 
    CAGGCCCCCC CACCCTCTTC ATCTGCATCC TCTTCCGCTG CTGCTGCTGC TGCATCCGCT 
    GCTGTGGCTG TGGCTGGATC GTCTGCCCCC AGTACCTCTG CAACCAACAA CCCCCCCGCT 
    GGCACGGCCA GCGCAGCCAC GTCGGAGGTT CACACTGTTG CCGAGCCACC AGCAGCTCAG 
    AGAGATGATT CGCCCATGGA TGTGGATCAG CCCTCTCCTT TGGAACAGGA CCAGCCAACA 
    TTAGGTGAGG AGAGCAGCAG TCAGCAGGAG CAGGAGGAGC GTTTGCCTGA CCTGCCGCTG 
    TTAAGTGAGC AGCTGCTTTT GGATGAGCTG TGGGACATGC TTGGAGAATG TCTGAAAGAG 
    CTTGAAGAGT CTCATGACCA ACATGCAGTT CTAGTGCTGC AGCCTGCAGT GGAGGCCTTT 
    TTCTTGGTGC ATGCAACAGA GCGGGAAAGC AAACCACCAG TTCGGGACAC TCGGGAGAGC 
    CAGCTGTCAC ACATCAAGGA TGAGCCTCCA CCCCTGTCAC CCGCCCCTCT CACACCCGCT 
    ACCCCCTCCT CTCTGGACCC CTTTTTTTCC AGAGAGCCTT CCTCTATGCA CATCTCTTCC 
    AACCTGCCTC CAGATACACA GAAGTTCCTC CGTTTCGCAG AGACCCACCG CACAGTGCTG 
    AATCAGATCC TGCGTCAATC CACCACTCAC CTGGCAGACG GGCCTTTCGC TGTTTTGGTT 
    GACTACATTC GCATCTTGGA CTTTGACGTC AAGCGCAAGT ATTTCCGTCA GGAGCTGGAG 
    CGTCTGGATG AAGGCTTGAG GAAAGAGGAT ATGGCCGTCC ATGTGAGGAG AGATCACGTC 
    TTTGAAGATT CTTACAGAGA GCTGCACCGC AAGAGCCCAG AAGACATGAA GAACAGATTG 
    TATATAGTAT TTGAAGGAGA GGAGGGTCAG GATGCAGGCG GGCTGTTAAG AGAGTGGTAC 
    ATGATCATCT CCAGAGAAAT GTTCAACCCC ATGTATGCCC TGTTTCGCAC CTCCCCCGGC 
    GATCGGGTCA CCTATACCAT CAACCCCTCC TCCCACTGCA ACCCCAACCA TCTCAGCTAC 
    TTCAAGTTTG TGGGACGTGT GGTGGCCAAA GCAGTCTACG ACAACAGGCT ATTAGAGTGT 
    TACTTTACAC GCAGCTTCTA CAAGCACATC CTGGGCAAGA GCGTTAGGTA TACAGACATG 
    GAAAGCGAAG ACTATCCCTT TTTCCAAGGT CTGGTTTATT TGTTGGAGAA CGATGTGTCG 
    ACTCTGGGCT ACGAGCTGAC ATTCAGCACA GAGGTCCAAG AGTTTGGCGT GTGTGAAGTG 
    AGAGATCTGA AGGCCAACGG TGCCAACATT CTTGTCACAG AAGAGAATAA GAAGGAATAT 
    GTGCATCTGG TCTGCCAGAT GAAGATGACA GGTGCAATCC GTAAACAGCT GGCTGCCTTC 
    CTGGAGGGCT TTTATGAGAT CATCCCCAAG AGACTGATCT CCATCTTCAC TGAGCAAGAG 
    CTGGAGCTGC TCATCTCCGG CCTGCCCACC ATTGACATCG ACGACCTCAA GGCCAATACT 
    GAATACCATA AATATCAGTC CAGTTCTATT CAGATCCAGT GGTTCTGGCG TGCTTTACGA 
    TCCTTCGACC AGGCTGACAG GGCCAAATTT CTGCAGTTTG TTACGGGCAC TTCCAAGGTG 
    CCACTGCAGG GCTTTGCTGC ACTGGAGGGA ATGAACGGCA TTCAGAAGTT CCAGATCCAC 
    CGAGATGACC GCTCCACTGA CCGCCTGCCC TCTGCGCACA CTTGCTTTAA CCAGTTGGAC 
    CTCCCAGCGT ACGAGAGCTA CGAGAAGTTA AGACACATGC TGCTGCTGGC CATTCAGGAA 
    TGTTCTGAAG GCTTCGGACT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_021473709.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329384.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa27858
    Clone ID Related Accession (Same CDS sequence) XM_021473709.1 , XM_001923865.5 , XM_001923865.6
    Accession Version XM_021473709.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGCTTCAC TCAGTCCAGA TATAGCTGAA AGTTCAGAGC CTGTGCCAGT GGGTGTGTCT 
    CAACTCGAGG GTTCGCCCAT GGATACGAGC AGTCCCGCTT CAGGAACGCA GGAAGAGCCG 
    GCTGCCAGCT CAGTTCCATC CAATCACATG TCTCAGGAGC TATCAGGAAG TGGTGACAGC 
    GGCCTCACAG ACAGGCAGAC AGATGCAGAA ACTGGATCAA CTTCTGTTTC ATCTCCTGGC 
    GAGACCATGC CTCGGAGTGA CAGTGCCGAT AGCCAGTCGC AGGCCATCCA GGAGGAGCCC 
    CTCCCTTCCA CCAGCAATGA AGAGGAGGAC CCTCTGGCAG GCATCAGTCT ACCTGAAGGC 
    GTGGATCCGT CTTTCTTGGC AGCACTGCCT GAAGACATTC GTCGGGAGGT GCTGCAGAAC 
    CAACTGGGAA TCAGACCTCC TTCTCGACCT CCTGTCGCCT CCACTCTGAC AACCACTGCT 
    AGTGCTGTGC TAGGAGGAGG GCCAGGGGTG ACCGAAGTCA GCCCTGAATT CTTAGCGGCT 
    CTCCCACCTG CAATTCAGGA AGAGGTCCTG GCCCAGCAGC GGGCAGAGCA GCAGCGTCGG 
    GAGTTATCCC AGCAACCCAC ACAGGGTGAT CAACCTCTGG ACCCAGTCAC ATTCATCCAG 
    ACCCTGCCCT CTGAGCTGCG GCGCTCCGTT CTGGAAGACA TGGAGGACAG TGTACTAGCG 
    GTCATGCCTC CAGATATTGC AGCAGAGGCT GCAGCACTGC GAAGGGAACA AGAGGCCAGA 
    CAGAGACAAC TGATGCACGA GCGCTTGTTC GGTCACAGCT CCTCTTCAGC TCTGTCTGCC 
    ATCTTACGCT CTCCCGCCTT CACCAGCCGC CTCGGGGGCA ACCGTGGGGT ACAGTACACC 
    CGGCTGGCTG TGCAAAGAGG AGGGACGTTT CAAATGGGAG GGAGTGCCAA TCATAGCAGA 
    CCCTCTAGTT CAAACGTTGA CTCTCTCCTG CGCCTGCGGG GGCGTCTCTT GTTGGACCAC 
    GAGGCCTTGT CCTGCCTTCT TGTGCTGCTT TTTGTGGACG AGCCCAAACT TAACACCAGC 
    CGCCTCCACC GAGTGCTGCG TAACTTGTGC TATCACTCTC AGACGCGTGG CTGGGTCATC 
    CGCAGTCTCC TGTCCATCCT GCAGCGCAGC AGTGAGAGCG AGCTGTGCCT GGAGACTGCA 
    CGCTTGGAGG AGTCCCGTGG CAAACGCTCA CTAGGCCAGG GCTCATCTAG TGGAGGAAAG 
    GGTTGTTCTT CATCCTCCCA GTCGGCTATA GCCTCTTCGT CATCGTCCTC CTCGTCCTCT 
    TCTTCTCTGG AGCTGCTGAA CAGGGTGGAG TCACGGAGTT CCAGTCAGCT CTCCTGGCTG 
    TCTGTATCAA TGGACGCAGC TCTTGGATGC AGAACCAACA TCTTTCAGAT CCAGCGAGTA 
    TCAGGCAGGA AACACGCAGA GAGGCATTCA GCAGGAGGGA GTGTGAGCGC AGGAGGACTC 
    ACAGGTGGAG TGTCAGGAAC AGGAATGACG TGTACAGGGG GAGGTGGGTC AACTGTTCAC 
    ATCCATCCAC AGGCTGCGCC GGTGGTCTGT CGACATGTAC TGGACACCCT CATCCAATTG 
    GCCAAGGTAT TTCCAAGTCA CTTCACTCAA CAACGCTGTA AAGATCCCCT ATCCTCCTCC 
    TCCTCTGAGT TGGACACTCG TCTTTGTGCC AGCTCAACAC CGACTATGAG TGCCAGTTGT 
    CGGTCAGGTG GCACCAGTCA GAGTAACTCC TCTGCTACCC CAAGTAGTCT CACTTCTATC 
    CACAATTCCC TGAGCGCCTG CTCTGGTACG CCACAGTCCC TAGGCATCTC CACCGACTTT 
    TGGGATCTCC TGGTCAAGCT GGACAACATG AATGTGAGCC GAAAGGGGAA GGCCTCCATG 
    AAGACTGTCC CTCTAGGGGG TGTCGGCGAG ACAGAGGGTG CGCTGTTCAG CCTTGAGGCG 
    TCTCCGCTGG GCCAGCTGAT GAATATGCTG TCCCACCCTG TGATCCGCCG TAGTTCCCTC 
    CTCACGGAGA AACTGCTACG TCTGCTGTCT CTGATCTCCA TCGCTCTGCC TGACAACAAG 
    GCCACGGAGG TTCCAGCCAG CCATCCAGTG CCTCAGACCC CAACTGCCAC GCCATCTGCA 
    CCTGCTCCTC AAACCACAGC AGGGTCAGGG ACCATCTCAG CAGTGGCAGC TACAGGCACT 
    ACGCAGGGTT CAGTCGGTTC AGTGGGTACA ACGCCATCTC AACCAGCCGC TACAATATCT 
    GTGGTTGCAG GCAGCACCAC CGCCTCTAGC ATTATTGCTG GAAAACTGAA GCCAGCAGTA 
    AGCATCACGG ACATTGAATC TAAACTGGCC TCTACTGGAC TTACGGAGAA GCAGCTGCAG 
    CTCTCTGTGG AGGTTCTGAC ATCTCACTCG TGTTCAGAGG AAGGTCTGGA GGACGCAGCC 
    AACATCTTGC TGCAGCTGTC CAGAGGGGAC GCACCGACCC GAGACACTGT GCTTAGACTT 
    CTGCTCAGCG GAGCACGTCA CCTTGGATAC ACTCTCTGCA AACAAATCGG CACTTTATTA 
    GCTGAGTTGA GGGAGTATAA TCTAGAGCAG CAGAGAAGAG CACGGGCTGA TGCTCAGTCT 
    CCTGAAGCTT CTCATGATGA CCCATCCATC ACCACACGCC TCAAGGGCAA GATGACCAGC 
    AGGTTTGACG GGTCAGAGAG TGTGGTGATT GTTGCTGCTC AGAAGCGCAC GTTAGGTGGC 
    CGGGAGCTTC AGTTGCCCTG CATGAGCTCG CTCACTTCCA AGACCTCAAC GCAGAAGTTT 
    TTCTTGCGGG TACTGCAGGT CATCATCCAG CTTAGAGAAG ACACGCGGCG CGCTAACAAG 
    AAAGCCAAGC AGACAGGAGG AAGACTAAGT TCAACCAGTC TGGGCTCGGC CAGTAGTATT 
    CAGGCTGCGG TCCGTCAGCT GGAGGCTGAA GCGGACGCCA TCATTCAGAT GGTGAGAGAG 
    GGACAGAGGG CACGCCGTCT ACAGCAGGCC CCCCCACCCT CTTCATCTGC ATCCTCTTCC 
    GCTGCTGCTG CTGCTGCATC CGCTGCTGTG GCTGTGGCTG GATCGTCTGC CCCCAGTACC 
    TCTGCAACCA ACAACCCCCC CGCTGGCACG GCCAGCGCAG CCACGTCGGA GGTTCACACT 
    GTTGCCGAGC CACCAGCAGC TCAGAGAGAT GATTCGCCCA TGGATGTGGA TCAGCCCTCT 
    CCTTTGGAAC AGGACCAGCC AACATTAGGT GAGGAGAGCA GCAGTCAGCA GGAGCAGGAG 
    GAGCGTTTGC CTGACCTGCC GCTGTTAAGT GAGCAGCTGC TTTTGGATGA GCTGTGGGAC 
    ATGCTTGGAG AATGTCTGAA AGAGCTTGAA GAGTCTCATG ACCAACATGC AGTTCTAGTG 
    CTGCAGCCTG CAGTGGAGGC CTTTTTCTTG GTGCATGCAA CAGAGCGGGA AAGCAAACCA 
    CCAGTTCGGG ACACTCGGGA GAGCCAGCTG TCACACATCA AGGATGAGCC TCCACCCCTG 
    TCACCCGCCC CTCTCACACC CGCTACCCCC TCCTCTCTGG ACCCCTTTTT TTCCAGAGAG 
    CCTTCCTCTA TGCACATCTC TTCCAACCTG CCTCCAGATA CACAGAAGTT CCTCCGTTTC 
    GCAGAGACCC ACCGCACAGT GCTGAATCAG ATCCTGCGTC AATCCACCAC TCACCTGGCA 
    GACGGGCCTT TCGCTGTTTT GGTTGACTAC ATTCGCATCT TGGACTTTGA CGTCAAGCGC 
    AAGTATTTCC GTCAGGAGCT GGAGCGTCTG GATGAAGGCT TGAGGAAAGA GGATATGGCC 
    GTCCATGTGA GGAGAGATCA CGTCTTTGAA GATTCTTACA GAGAGCTGCA CCGCAAGAGC 
    CCAGAAGACA TGAAGAACAG ATTGTATATA GTATTTGAAG GAGAGGAGGG TCAGGATGCA 
    GGCGGGCTGT TAAGAGAGTG GTACATGATC ATCTCCAGAG AAATGTTCAA CCCCATGTAT 
    GCCCTGTTTC GCACCTCCCC CGGCGATCGG GTCACCTATA CCATCAACCC CTCCTCCCAC 
    TGCAACCCCA ACCATCTCAG CTACTTCAAG TTTGTGGGAC GTGTGGTGGC CAAAGCAGTC 
    TACGACAACA GGCTATTAGA GTGTTACTTT ACACGCAGCT TCTACAAGCA CATCCTGGGC 
    AAGAGCGTTA GGTATACAGA CATGGAAAGC GAAGACTATC CCTTTTTCCA AGGTCTGGTT 
    TATTTGTTGG AGAACGATGT GTCGACTCTG GGCTACGAGC TGACATTCAG CACAGAGGTC 
    CAAGAGTTTG GCGTGTGTGA AGTGAGAGAT CTGAAGGCCA ACGGTGCCAA CATTCTTGTC 
    ACAGAAGAGA ATAAGAAGGA ATATGTGCAT CTGGTCTGCC AGATGAAGAT GACAGGTGCA 
    ATCCGTAAAC AGCTGGCTGC CTTCCTGGAG GGCTTTTATG AGATCATCCC CAAGAGACTG 
    ATCTCCATCT TCACTGAGCA AGAGCTGGAG CTGCTCATCT CCGGCCTGCC CACCATTGAC 
    ATCGACGACC TCAAGGCCAA TACTGAATAC CATAAATATC AGTCCAGTTC TATTCAGATC 
    CAGTGGTTCT GGCGTGCTTT ACGATCCTTC GACCAGGCTG ACAGGGCCAA ATTTCTGCAG 
    TTTGTTACGG GCACTTCCAA GGTGCCACTG CAGGGCTTTG CTGCACTGGA GGGAATGAAC 
    GGCATTCAGA AGTTCCAGAT CCACCGAGAT GACCGCTCCA CTGACCGCCT GCCCTCTGCG 
    CACACTTGCT TTAACCAGTT GGACCTCCCA GCGTACGAGA GCTACGAGAA GTTAAGACAC 
    ATGCTGCTGC TGGCCATTCA GGAATGTTCT GAAGGCTTCG GACTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329384.1
    CDS142..13812
    Translation

    Target ORF information:

    RefSeq Version XM_021473709.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021473709.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGCTTCAC TCAGTCCAGA TATAGCTGAA AGTTCAGAGC CTGTGCCAGT GGGTGTGTCT 
    CAACTCGAGG GTTCGCCCAT GGATACGAGC AGTCCCGCTT CAGGAACGCA GGAAGAGCCG 
    GCTGCCAGCT CAGTTCCATC CAATCACATG TCTCAGGAGC TATCAGGAAG TGGTGACAGC 
    GGCCTCACAG ACAGGCAGAC AGATGCAGAA ACTGGATCAA CTTCTGTTTC ATCTCCTGGC 
    GAGACCATGC CTCGGAGTGA CAGTGCCGAT AGCCAGTCGC AGGCCATCCA GGAGGAGCCC 
    CTCCCTTCCA CCAGCAATGA AGAGGAGGAC CCTCTGGCAG GCATCAGTCT ACCTGAAGGC 
    GTGGATCCGT CTTTCTTGGC AGCACTGCCT GAAGACATTC GTCGGGAGGT GCTGCAGAAC 
    CAACTGGGAA TCAGACCTCC TTCTCGACCT CCTGTCGCCT CCACTCTGAC AACCACTGCT 
    AGTGCTGTGC TAGGAGGAGG GCCAGGGGTG ACCGAAGTCA GCCCTGAATT CTTAGCGGCT 
    CTCCCACCTG CAATTCAGGA AGAGGTCCTG GCCCAGCAGC GGGCAGAGCA GCAGCGTCGG 
    GAGTTATCCC AGCAACCCAC ACAGGGTGAT CAACCTCTGG ACCCAGTCAC ATTCATCCAG 
    ACCCTGCCCT CTGAGCTGCG GCGCTCCGTT CTGGAAGACA TGGAGGACAG TGTACTAGCG 
    GTCATGCCTC CAGATATTGC AGCAGAGGCT GCAGCACTGC GAAGGGAACA AGAGGCCAGA 
    CAGAGACAAC TGATGCACGA GCGCTTGTTC GGTCACAGCT CCTCTTCAGC TCTGTCTGCC 
    ATCTTACGCT CTCCCGCCTT CACCAGCCGC CTCGGGGGCA ACCGTGGGGT ACAGTACACC 
    CGGCTGGCTG TGCAAAGAGG AGGGACGTTT CAAATGGGAG GGAGTGCCAA TCATAGCAGA 
    CCCTCTAGTT CAAACGTTGA CTCTCTCCTG CGCCTGCGGG GGCGTCTCTT GTTGGACCAC 
    GAGGCCTTGT CCTGCCTTCT TGTGCTGCTT TTTGTGGACG AGCCCAAACT TAACACCAGC 
    CGCCTCCACC GAGTGCTGCG TAACTTGTGC TATCACTCTC AGACGCGTGG CTGGGTCATC 
    CGCAGTCTCC TGTCCATCCT GCAGCGCAGC AGTGAGAGCG AGCTGTGCCT GGAGACTGCA 
    CGCTTGGAGG AGTCCCGTGG CAAACGCTCA CTAGGCCAGG GCTCATCTAG TGGAGGAAAG 
    GGTTGTTCTT CATCCTCCCA GTCGGCTATA GCCTCTTCGT CATCGTCCTC CTCGTCCTCT 
    TCTTCTCTGG AGCTGCTGAA CAGGGTGGAG TCACGGAGTT CCAGTCAGCT CTCCTGGCTG 
    TCTGTATCAA TGGACGCAGC TCTTGGATGC AGAACCAACA TCTTTCAGAT CCAGCGAGTA 
    TCAGGCAGGA AACACGCAGA GAGGCATTCA GCAGGAGGGA GTGTGAGCGC AGGAGGACTC 
    ACAGGTGGAG TGTCAGGAAC AGGAATGACG TGTACAGGGG GAGGTGGGTC AACTGTTCAC 
    ATCCATCCAC AGGCTGCGCC GGTGGTCTGT CGACATGTAC TGGACACCCT CATCCAATTG 
    GCCAAGGTAT TTCCAAGTCA CTTCACTCAA CAACGCTGTA AAGATCCCCT ATCCTCCTCC 
    TCCTCTGAGT TGGACACTCG TCTTTGTGCC AGCTCAACAC CGACTATGAG TGCCAGTTGT 
    CGGTCAGGTG GCACCAGTCA GAGTAACTCC TCTGCTACCC CAAGTAGTCT CACTTCTATC 
    CACAATTCCC TGAGCGCCTG CTCTGGTACG CCACAGTCCC TAGGCATCTC CACCGACTTT 
    TGGGATCTCC TGGTCAAGCT GGACAACATG AATGTGAGCC GAAAGGGGAA GGCCTCCATG 
    AAGACTGTCC CTCTAGGGGG TGTCGGCGAG ACAGAGGGTG CGCTGTTCAG CCTTGAGGCG 
    TCTCCGCTGG GCCAGCTGAT GAATATGCTG TCCCACCCTG TGATCCGCCG TAGTTCCCTC 
    CTCACGGAGA AACTGCTACG TCTGCTGTCT CTGATCTCCA TCGCTCTGCC TGACAACAAG 
    GCCACGGAGG TTCCAGCCAG CCATCCAGTG CCTCAGACCC CAACTGCCAC GCCATCTGCA 
    CCTGCTCCTC AAACCACAGC AGGGTCAGGG ACCATCTCAG CAGTGGCAGC TACAGGCACT 
    ACGCAGGGTT CAGTCGGTTC AGTGGGTACA ACGCCATCTC AACCAGCCGC TACAATATCT 
    GTGGTTGCAG GCAGCACCAC CGCCTCTAGC ATTATTGCTG GAAAACTGAA GCCAGCAGTA 
    AGCATCACGG ACATTGAATC TAAACTGGCC TCTACTGGAC TTACGGAGAA GCAGCTGCAG 
    CTCTCTGTGG AGGTTCTGAC ATCTCACTCG TGTTCAGAGG AAGGTCTGGA GGACGCAGCC 
    AACATCTTGC TGCAGCTGTC CAGAGGGGAC GCACCGACCC GAGACACTGT GCTTAGACTT 
    CTGCTCAGCG GAGCACGTCA CCTTGGATAC ACTCTCTGCA AACAAATCGG CACTTTATTA 
    GCTGAGTTGA GGGAGTATAA TCTAGAGCAG CAGAGAAGAG CACGGGCTGA TGCTCAGTCT 
    CCTGAAGCTT CTCATGATGA CCCATCCATC ACCACACGCC TCAAGGGCAA GATGACCAGC 
    AGGTTTGACG GGTCAGAGAG TGTGGTGATT GTTGCTGCTC AGAAGCGCAC GTTAGGTGGC 
    CGGGAGCTTC AGTTGCCCTG CATGAGCTCG CTCACTTCCA AGACCTCAAC GCAGAAGTTT 
    TTCTTGCGGG TACTGCAGGT CATCATCCAG CTTAGAGAAG ACACGCGGCG CGCTAACAAG 
    AAAGCCAAGC AGACAGGAGG AAGACTAAGT TCAACCAGTC TGGGCTCGGC CAGTAGTATT 
    CAGGCTGCGG TCCGTCAGCT GGAGGCTGAA GCGGACGCCA TCATTCAGAT GGTGAGAGAG 
    GGACAGAGGG CACGCCGTCT ACAGCAGGCC CCCCCACCCT CTTCATCTGC ATCCTCTTCC 
    GCTGCTGCTG CTGCTGCATC CGCTGCTGTG GCTGTGGCTG GATCGTCTGC CCCCAGTACC 
    TCTGCAACCA ACAACCCCCC CGCTGGCACG GCCAGCGCAG CCACGTCGGA GGTTCACACT 
    GTTGCCGAGC CACCAGCAGC TCAGAGAGAT GATTCGCCCA TGGATGTGGA TCAGCCCTCT 
    CCTTTGGAAC AGGACCAGCC AACATTAGGT GAGGAGAGCA GCAGTCAGCA GGAGCAGGAG 
    GAGCGTTTGC CTGACCTGCC GCTGTTAAGT GAGCAGCTGC TTTTGGATGA GCTGTGGGAC 
    ATGCTTGGAG AATGTCTGAA AGAGCTTGAA GAGTCTCATG ACCAACATGC AGTTCTAGTG 
    CTGCAGCCTG CAGTGGAGGC CTTTTTCTTG GTGCATGCAA CAGAGCGGGA AAGCAAACCA 
    CCAGTTCGGG ACACTCGGGA GAGCCAGCTG TCACACATCA AGGATGAGCC TCCACCCCTG 
    TCACCCGCCC CTCTCACACC CGCTACCCCC TCCTCTCTGG ACCCCTTTTT TTCCAGAGAG 
    CCTTCCTCTA TGCACATCTC TTCCAACCTG CCTCCAGATA CACAGAAGTT CCTCCGTTTC 
    GCAGAGACCC ACCGCACAGT GCTGAATCAG ATCCTGCGTC AATCCACCAC TCACCTGGCA 
    GACGGGCCTT TCGCTGTTTT GGTTGACTAC ATTCGCATCT TGGACTTTGA CGTCAAGCGC 
    AAGTATTTCC GTCAGGAGCT GGAGCGTCTG GATGAAGGCT TGAGGAAAGA GGATATGGCC 
    GTCCATGTGA GGAGAGATCA CGTCTTTGAA GATTCTTACA GAGAGCTGCA CCGCAAGAGC 
    CCAGAAGACA TGAAGAACAG ATTGTATATA GTATTTGAAG GAGAGGAGGG TCAGGATGCA 
    GGCGGGCTGT TAAGAGAGTG GTACATGATC ATCTCCAGAG AAATGTTCAA CCCCATGTAT 
    GCCCTGTTTC GCACCTCCCC CGGCGATCGG GTCACCTATA CCATCAACCC CTCCTCCCAC 
    TGCAACCCCA ACCATCTCAG CTACTTCAAG TTTGTGGGAC GTGTGGTGGC CAAAGCAGTC 
    TACGACAACA GGCTATTAGA GTGTTACTTT ACACGCAGCT TCTACAAGCA CATCCTGGGC 
    AAGAGCGTTA GGTATACAGA CATGGAAAGC GAAGACTATC CCTTTTTCCA AGGTCTGGTT 
    TATTTGTTGG AGAACGATGT GTCGACTCTG GGCTACGAGC TGACATTCAG CACAGAGGTC 
    CAAGAGTTTG GCGTGTGTGA AGTGAGAGAT CTGAAGGCCA ACGGTGCCAA CATTCTTGTC 
    ACAGAAGAGA ATAAGAAGGA ATATGTGCAT CTGGTCTGCC AGATGAAGAT GACAGGTGCA 
    ATCCGTAAAC AGCTGGCTGC CTTCCTGGAG GGCTTTTATG AGATCATCCC CAAGAGACTG 
    ATCTCCATCT TCACTGAGCA AGAGCTGGAG CTGCTCATCT CCGGCCTGCC CACCATTGAC 
    ATCGACGACC TCAAGGCCAA TACTGAATAC CATAAATATC AGTCCAGTTC TATTCAGATC 
    CAGTGGTTCT GGCGTGCTTT ACGATCCTTC GACCAGGCTG ACAGGGCCAA ATTTCTGCAG 
    TTTGTTACGG GCACTTCCAA GGTGCCACTG CAGGGCTTTG CTGCACTGGA GGGAATGAAC 
    GGCATTCAGA AGTTCCAGAT CCACCGAGAT GACCGCTCCA CTGACCGCCT GCCCTCTGCG 
    CACACTTGCT TTAACCAGTT GGACCTCCCA GCGTACGAGA GCTACGAGAA GTTAAGACAC 
    ATGCTGCTGC TGGCCATTCA GGAATGTTCT GAAGGCTTCG GACTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_001923865.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001923900.2 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa27858
    Clone ID Related Accession (Same CDS sequence) XM_021473709.1 , XM_001923865.5 , XM_001923865.6
    Accession Version XM_001923865.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001923865.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    13501
    13561
    13621
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGCTTCAC TCAGTCCAGA TATAGCTGAA AGTTCAGAGC CTGTGCCAGT GGGTGTGTCT 
    CAACTCGAGG GTTCGCCCAT GGATACGAGC AGTCCCGCTT CAGGAACGCA GGAAGAGCCG 
    GCTGCCAGCT CAGTTCCATC CAATCACATG TCTCAGGAGC TATCAGGAAG TGGTGACAGC 
    GGCCTCACAG ACAGGCAGAC AGATGCAGAA ACTGGATCAA CTTCTGTTTC ATCTCCTGGC 
    GAGACCATGC CTCGGAGTGA CAGTGCCGAT AGCCAGTCGC AGGCCATCCA GGAGGAGCCC 
    CTCCCTTCCA CCAGCAATGA AGAGGAGGAC CCTCTGGCAG GCATCAGTCT ACCTGAAGGC 
    GTGGATCCGT CTTTCTTGGC AGCACTGCCT GAAGACATTC GTCGGGAGGT GCTGCAGAAC 
    CAACTGGGAA TCAGACCTCC TTCTCGACCT CCTGTCGCCT CCACTCTGAC AACCACTGCT 
    AGTGCTGTGC TAGGAGGAGG GCCAGGGGTG ACCGAAGTCA GCCCTGAATT CTTAGCGGCT 
    CTCCCACCTG CAATTCAGGA AGAGGTCCTG GCCCAGCAGC GGGCAGAGCA GCAGCGTCGG 
    GAGTTATCCC AGCAACCCAC ACAGGGTGAT CAACCTCTGG ACCCAGTCAC ATTCATCCAG 
    ACCCTGCCCT CTGAGCTGCG GCGCTCCGTT CTGGAAGACA TGGAGGACAG TGTACTAGCG 
    GTCATGCCTC CAGATATTGC AGCAGAGGCT GCAGCACTGC GAAGGGAACA AGAGGCCAGA 
    CAGAGACAAC TGATGCACGA GCGCTTGTTC GGTCACAGCT CCTCTTCAGC TCTGTCTGCC 
    ATCTTACGCT CTCCCGCCTT CACCAGCCGC CTCGGGGGCA ACCGTGGGGT ACAGTACACC 
    CGGCTGGCTG TGCAAAGAGG AGGGACGTTT CAAATGGGAG GGAGTGCCAA TCATAGCAGA 
    CCCTCTAGTT CAAACGTTGA CTCTCTCCTG CGCCTGCGGG GGCGTCTCTT GTTGGACCAC 
    GAGGCCTTGT CCTGCCTTCT TGTGCTGCTT TTTGTGGACG AGCCCAAACT TAACACCAGC 
    CGCCTCCACC GAGTGCTGCG TAACTTGTGC TATCACTCTC AGACGCGTGG CTGGGTCATC 
    CGCAGTCTCC TGTCCATCCT GCAGCGCAGC AGTGAGAGCG AGCTGTGCCT GGAGACTGCA 
    CGCTTGGAGG AGTCCCGTGG CAAACGCTCA CTAGGCCAGG GCTCATCTAG TGGAGGAAAG 
    GGTTGTTCTT CATCCTCCCA GTCGGCTATA GCCTCTTCGT CATCGTCCTC CTCGTCCTCT 
    TCTTCTCTGG AGCTGCTGAA CAGGGTGGAG TCACGGAGTT CCAGTCAGCT CTCCTGGCTG 
    TCTGTATCAA TGGACGCAGC TCTTGGATGC AGAACCAACA TCTTTCAGAT CCAGCGAGTA 
    TCAGGCAGGA AACACGCAGA GAGGCATTCA GCAGGAGGGA GTGTGAGCGC AGGAGGACTC 
    ACAGGTGGAG TGTCAGGAAC AGGAATGACG TGTACAGGGG GAGGTGGGTC AACTGTTCAC 
    ATCCATCCAC AGGCTGCGCC GGTGGTCTGT CGACATGTAC TGGACACCCT CATCCAATTG 
    GCCAAGGTAT TTCCAAGTCA CTTCACTCAA CAACGCTGTA AAGATCCCCT ATCCTCCTCC 
    TCCTCTGAGT TGGACACTCG TCTTTGTGCC AGCTCAACAC CGACTATGAG TGCCAGTTGT 
    CGGTCAGGTG GCACCAGTCA GAGTAACTCC TCTGCTACCC CAAGTAGTCT CACTTCTATC 
    CACAATTCCC TGAGCGCCTG CTCTGGTACG CCACAGTCCC TAGGCATCTC CACCGACTTT 
    TGGGATCTCC TGGTCAAGCT GGACAACATG AATGTGAGCC GAAAGGGGAA GGCCTCCATG 
    AAGACTGTCC CTCTAGGGGG TGTCGGCGAG ACAGAGGGTG CGCTGTTCAG CCTTGAGGCG 
    TCTCCGCTGG GCCAGCTGAT GAATATGCTG TCCCACCCTG TGATCCGCCG TAGTTCCCTC 
    CTCACGGAGA AACTGCTACG TCTGCTGTCT CTGATCTCCA TCGCTCTGCC TGACAACAAG 
    GCCACGGAGG TTCCAGCCAG CCATCCAGTG CCTCAGACCC CAACTGCCAC GCCATCTGCA 
    CCTGCTCCTC AAACCACAGC AGGGTCAGGG ACCATCTCAG CAGTGGCAGC TACAGGCACT 
    ACGCAGGGTT CAGTCGGTTC AGTGGGTACA ACGCCATCTC AACCAGCCGC TACAATATCT 
    GTGGTTGCAG GCAGCACCAC CGCCTCTAGC ATTATTGCTG GAAAACTGAA GCCAGCAGTA 
    AGCATCACGG ACATTGAATC TAAACTGGCC TCTACTGGAC TTACGGAGAA GCAGCTGCAG 
    CTCTCTGTGG AGGTTCTGAC ATCTCACTCG TGTTCAGAGG AAGGTCTGGA GGACGCAGCC 
    AACATCTTGC TGCAGCTGTC CAGAGGGGAC GCACCGACCC GAGACACTGT GCTTAGACTT 
    CTGCTCAGCG GAGCACGTCA CCTTGGATAC ACTCTCTGCA AACAAATCGG CACTTTATTA 
    GCTGAGTTGA GGGAGTATAA TCTAGAGCAG CAGAGAAGAG CACGGGCTGA TGCTCAGTCT 
    CCTGAAGCTT CTCATGATGA CCCATCCATC ACCACACGCC TCAAGGGCAA GATGACCAGC 
    AGGTTTGACG GGTCAGAGAG TGTGGTGATT GTTGCTGCTC AGAAGCGCAC GTTAGGTGGC 
    CGGGAGCTTC AGTTGCCCTG CATGAGCTCG CTCACTTCCA AGACCTCAAC GCAGAAGTTT 
    TTCTTGCGGG TACTGCAGGT CATCATCCAG CTTAGAGAAG ACACGCGGCG CGCTAACAAG 
    AAAGCCAAGC AGACAGGAGG AAGACTAAGT TCAACCAGTC TGGGCTCGGC CAGTAGTATT 
    CAGGCTGCGG TCCGTCAGCT GGAGGCTGAA GCGGACGCCA TCATTCAGAT GGTGAGAGAG 
    GGACAGAGGG CACGCCGTCT ACAGCAGGCC CCCCCACCCT CTTCATCTGC ATCCTCTTCC 
    GCTGCTGCTG CTGCTGCATC CGCTGCTGTG GCTGTGGCTG GATCGTCTGC CCCCAGTACC 
    TCTGCAACCA ACAACCCCCC CGCTGGCACG GCCAGCGCAG CCACGTCGGA GGTTCACACT 
    GTTGCCGAGC CACCAGCAGC TCAGAGAGAT GATTCGCCCA TGGATGTGGA TCAGCCCTCT 
    CCTTTGGAAC AGGACCAGCC AACATTAGGT GAGGAGAGCA GCAGTCAGCA GGAGCAGGAG 
    GAGCGTTTGC CTGACCTGCC GCTGTTAAGT GAGCAGCTGC TTTTGGATGA GCTGTGGGAC 
    ATGCTTGGAG AATGTCTGAA AGAGCTTGAA GAGTCTCATG ACCAACATGC AGTTCTAGTG 
    CTGCAGCCTG CAGTGGAGGC CTTTTTCTTG GTGCATGCAA CAGAGCGGGA AAGCAAACCA 
    CCAGTTCGGG ACACTCGGGA GAGCCAGCTG TCACACATCA AGGATGAGCC TCCACCCCTG 
    TCACCCGCCC CTCTCACACC CGCTACCCCC TCCTCTCTGG ACCCCTTTTT TTCCAGAGAG 
    CCTTCCTCTA TGCACATCTC TTCCAACCTG CCTCCAGATA CACAGAAGTT CCTCCGTTTC 
    GCAGAGACCC ACCGCACAGT GCTGAATCAG ATCCTGCGTC AATCCACCAC TCACCTGGCA 
    GACGGGCCTT TCGCTGTTTT GGTTGACTAC ATTCGCATCT TGGACTTTGA CGTCAAGCGC 
    AAGTATTTCC GTCAGGAGCT GGAGCGTCTG GATGAAGGCT TGAGGAAAGA GGATATGGCC 
    GTCCATGTGA GGAGAGATCA CGTCTTTGAA GATTCTTACA GAGAGCTGCA CCGCAAGAGC 
    CCAGAAGACA TGAAGAACAG ATTGTATATA GTATTTGAAG GAGAGGAGGG TCAGGATGCA 
    GGCGGGCTGT TAAGAGAGTG GTACATGATC ATCTCCAGAG AAATGTTCAA CCCCATGTAT 
    GCCCTGTTTC GCACCTCCCC CGGCGATCGG GTCACCTATA CCATCAACCC CTCCTCCCAC 
    TGCAACCCCA ACCATCTCAG CTACTTCAAG TTTGTGGGAC GTGTGGTGGC CAAAGCAGTC 
    TACGACAACA GGCTATTAGA GTGTTACTTT ACACGCAGCT TCTACAAGCA CATCCTGGGC 
    AAGAGCGTTA GGTATACAGA CATGGAAAGC GAAGACTATC CCTTTTTCCA AGGTCTGGTT 
    TATTTGTTGG AGAACGATGT GTCGACTCTG GGCTACGAGC TGACATTCAG CACAGAGGTC 
    CAAGAGTTTG GCGTGTGTGA AGTGAGAGAT CTGAAGGCCA ACGGTGCCAA CATTCTTGTC 
    ACAGAAGAGA ATAAGAAGGA ATATGTGCAT CTGGTCTGCC AGATGAAGAT GACAGGTGCA 
    ATCCGTAAAC AGCTGGCTGC CTTCCTGGAG GGCTTTTATG AGATCATCCC CAAGAGACTG 
    ATCTCCATCT TCACTGAGCA AGAGCTGGAG CTGCTCATCT CCGGCCTGCC CACCATTGAC 
    ATCGACGACC TCAAGGCCAA TACTGAATAC CATAAATATC AGTCCAGTTC TATTCAGATC 
    CAGTGGTTCT GGCGTGCTTT ACGATCCTTC GACCAGGCTG ACAGGGCCAA ATTTCTGCAG 
    TTTGTTACGG GCACTTCCAA GGTGCCACTG CAGGGCTTTG CTGCACTGGA GGGAATGAAC 
    GGCATTCAGA AGTTCCAGAT CCACCGAGAT GACCGCTCCA CTGACCGCCT GCCCTCTGCG 
    CACACTTGCT TTAACCAGTT GGACCTCCCA GCGTACGAGA GCTACGAGAA GTTAAGACAC 
    ATGCTGCTGC TGGCCATTCA GGAATGTTCT GAAGGCTTCG GACTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001923900.2
    CDS149..13819
    Translation

    Target ORF information:

    RefSeq Version XM_001923865.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001923865.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGCTTCAC TCAGTCCAGA TATAGCTGAA AGTTCAGAGC CTGTGCCAGT GGGTGTGTCT 
    CAACTCGAGG GTTCGCCCAT GGATACGAGC AGTCCCGCTT CAGGAACGCA GGAAGAGCCG 
    GCTGCCAGCT CAGTTCCATC CAATCACATG TCTCAGGAGC TATCAGGAAG TGGTGACAGC 
    GGCCTCACAG ACAGGCAGAC AGATGCAGAA ACTGGATCAA CTTCTGTTTC ATCTCCTGGC 
    GAGACCATGC CTCGGAGTGA CAGTGCCGAT AGCCAGTCGC AGGCCATCCA GGAGGAGCCC 
    CTCCCTTCCA CCAGCAATGA AGAGGAGGAC CCTCTGGCAG GCATCAGTCT ACCTGAAGGC 
    GTGGATCCGT CTTTCTTGGC AGCACTGCCT GAAGACATTC GTCGGGAGGT GCTGCAGAAC 
    CAACTGGGAA TCAGACCTCC TTCTCGACCT CCTGTCGCCT CCACTCTGAC AACCACTGCT 
    AGTGCTGTGC TAGGAGGAGG GCCAGGGGTG ACCGAAGTCA GCCCTGAATT CTTAGCGGCT 
    CTCCCACCTG CAATTCAGGA AGAGGTCCTG GCCCAGCAGC GGGCAGAGCA GCAGCGTCGG 
    GAGTTATCCC AGCAACCCAC ACAGGGTGAT CAACCTCTGG ACCCAGTCAC ATTCATCCAG 
    ACCCTGCCCT CTGAGCTGCG GCGCTCCGTT CTGGAAGACA TGGAGGACAG TGTACTAGCG 
    GTCATGCCTC CAGATATTGC AGCAGAGGCT GCAGCACTGC GAAGGGAACA AGAGGCCAGA 
    CAGAGACAAC TGATGCACGA GCGCTTGTTC GGTCACAGCT CCTCTTCAGC TCTGTCTGCC 
    ATCTTACGCT CTCCCGCCTT CACCAGCCGC CTCGGGGGCA ACCGTGGGGT ACAGTACACC 
    CGGCTGGCTG TGCAAAGAGG AGGGACGTTT CAAATGGGAG GGAGTGCCAA TCATAGCAGA 
    CCCTCTAGTT CAAACGTTGA CTCTCTCCTG CGCCTGCGGG GGCGTCTCTT GTTGGACCAC 
    GAGGCCTTGT CCTGCCTTCT TGTGCTGCTT TTTGTGGACG AGCCCAAACT TAACACCAGC 
    CGCCTCCACC GAGTGCTGCG TAACTTGTGC TATCACTCTC AGACGCGTGG CTGGGTCATC 
    CGCAGTCTCC TGTCCATCCT GCAGCGCAGC AGTGAGAGCG AGCTGTGCCT GGAGACTGCA 
    CGCTTGGAGG AGTCCCGTGG CAAACGCTCA CTAGGCCAGG GCTCATCTAG TGGAGGAAAG 
    GGTTGTTCTT CATCCTCCCA GTCGGCTATA GCCTCTTCGT CATCGTCCTC CTCGTCCTCT 
    TCTTCTCTGG AGCTGCTGAA CAGGGTGGAG TCACGGAGTT CCAGTCAGCT CTCCTGGCTG 
    TCTGTATCAA TGGACGCAGC TCTTGGATGC AGAACCAACA TCTTTCAGAT CCAGCGAGTA 
    TCAGGCAGGA AACACGCAGA GAGGCATTCA GCAGGAGGGA GTGTGAGCGC AGGAGGACTC 
    ACAGGTGGAG TGTCAGGAAC AGGAATGACG TGTACAGGGG GAGGTGGGTC AACTGTTCAC 
    ATCCATCCAC AGGCTGCGCC GGTGGTCTGT CGACATGTAC TGGACACCCT CATCCAATTG 
    GCCAAGGTAT TTCCAAGTCA CTTCACTCAA CAACGCTGTA AAGATCCCCT ATCCTCCTCC 
    TCCTCTGAGT TGGACACTCG TCTTTGTGCC AGCTCAACAC CGACTATGAG TGCCAGTTGT 
    CGGTCAGGTG GCACCAGTCA GAGTAACTCC TCTGCTACCC CAAGTAGTCT CACTTCTATC 
    CACAATTCCC TGAGCGCCTG CTCTGGTACG CCACAGTCCC TAGGCATCTC CACCGACTTT 
    TGGGATCTCC TGGTCAAGCT GGACAACATG AATGTGAGCC GAAAGGGGAA GGCCTCCATG 
    AAGACTGTCC CTCTAGGGGG TGTCGGCGAG ACAGAGGGTG CGCTGTTCAG CCTTGAGGCG 
    TCTCCGCTGG GCCAGCTGAT GAATATGCTG TCCCACCCTG TGATCCGCCG TAGTTCCCTC 
    CTCACGGAGA AACTGCTACG TCTGCTGTCT CTGATCTCCA TCGCTCTGCC TGACAACAAG 
    GCCACGGAGG TTCCAGCCAG CCATCCAGTG CCTCAGACCC CAACTGCCAC GCCATCTGCA 
    CCTGCTCCTC AAACCACAGC AGGGTCAGGG ACCATCTCAG CAGTGGCAGC TACAGGCACT 
    ACGCAGGGTT CAGTCGGTTC AGTGGGTACA ACGCCATCTC AACCAGCCGC TACAATATCT 
    GTGGTTGCAG GCAGCACCAC CGCCTCTAGC ATTATTGCTG GAAAACTGAA GCCAGCAGTA 
    AGCATCACGG ACATTGAATC TAAACTGGCC TCTACTGGAC TTACGGAGAA GCAGCTGCAG 
    CTCTCTGTGG AGGTTCTGAC ATCTCACTCG TGTTCAGAGG AAGGTCTGGA GGACGCAGCC 
    AACATCTTGC TGCAGCTGTC CAGAGGGGAC GCACCGACCC GAGACACTGT GCTTAGACTT 
    CTGCTCAGCG GAGCACGTCA CCTTGGATAC ACTCTCTGCA AACAAATCGG CACTTTATTA 
    GCTGAGTTGA GGGAGTATAA TCTAGAGCAG CAGAGAAGAG CACGGGCTGA TGCTCAGTCT 
    CCTGAAGCTT CTCATGATGA CCCATCCATC ACCACACGCC TCAAGGGCAA GATGACCAGC 
    AGGTTTGACG GGTCAGAGAG TGTGGTGATT GTTGCTGCTC AGAAGCGCAC GTTAGGTGGC 
    CGGGAGCTTC AGTTGCCCTG CATGAGCTCG CTCACTTCCA AGACCTCAAC GCAGAAGTTT 
    TTCTTGCGGG TACTGCAGGT CATCATCCAG CTTAGAGAAG ACACGCGGCG CGCTAACAAG 
    AAAGCCAAGC AGACAGGAGG AAGACTAAGT TCAACCAGTC TGGGCTCGGC CAGTAGTATT 
    CAGGCTGCGG TCCGTCAGCT GGAGGCTGAA GCGGACGCCA TCATTCAGAT GGTGAGAGAG 
    GGACAGAGGG CACGCCGTCT ACAGCAGGCC CCCCCACCCT CTTCATCTGC ATCCTCTTCC 
    GCTGCTGCTG CTGCTGCATC CGCTGCTGTG GCTGTGGCTG GATCGTCTGC CCCCAGTACC 
    TCTGCAACCA ACAACCCCCC CGCTGGCACG GCCAGCGCAG CCACGTCGGA GGTTCACACT 
    GTTGCCGAGC CACCAGCAGC TCAGAGAGAT GATTCGCCCA TGGATGTGGA TCAGCCCTCT 
    CCTTTGGAAC AGGACCAGCC AACATTAGGT GAGGAGAGCA GCAGTCAGCA GGAGCAGGAG 
    GAGCGTTTGC CTGACCTGCC GCTGTTAAGT GAGCAGCTGC TTTTGGATGA GCTGTGGGAC 
    ATGCTTGGAG AATGTCTGAA AGAGCTTGAA GAGTCTCATG ACCAACATGC AGTTCTAGTG 
    CTGCAGCCTG CAGTGGAGGC CTTTTTCTTG GTGCATGCAA CAGAGCGGGA AAGCAAACCA 
    CCAGTTCGGG ACACTCGGGA GAGCCAGCTG TCACACATCA AGGATGAGCC TCCACCCCTG 
    TCACCCGCCC CTCTCACACC CGCTACCCCC TCCTCTCTGG ACCCCTTTTT TTCCAGAGAG 
    CCTTCCTCTA TGCACATCTC TTCCAACCTG CCTCCAGATA CACAGAAGTT CCTCCGTTTC 
    GCAGAGACCC ACCGCACAGT GCTGAATCAG ATCCTGCGTC AATCCACCAC TCACCTGGCA 
    GACGGGCCTT TCGCTGTTTT GGTTGACTAC ATTCGCATCT TGGACTTTGA CGTCAAGCGC 
    AAGTATTTCC GTCAGGAGCT GGAGCGTCTG GATGAAGGCT TGAGGAAAGA GGATATGGCC 
    GTCCATGTGA GGAGAGATCA CGTCTTTGAA GATTCTTACA GAGAGCTGCA CCGCAAGAGC 
    CCAGAAGACA TGAAGAACAG ATTGTATATA GTATTTGAAG GAGAGGAGGG TCAGGATGCA 
    GGCGGGCTGT TAAGAGAGTG GTACATGATC ATCTCCAGAG AAATGTTCAA CCCCATGTAT 
    GCCCTGTTTC GCACCTCCCC CGGCGATCGG GTCACCTATA CCATCAACCC CTCCTCCCAC 
    TGCAACCCCA ACCATCTCAG CTACTTCAAG TTTGTGGGAC GTGTGGTGGC CAAAGCAGTC 
    TACGACAACA GGCTATTAGA GTGTTACTTT ACACGCAGCT TCTACAAGCA CATCCTGGGC 
    AAGAGCGTTA GGTATACAGA CATGGAAAGC GAAGACTATC CCTTTTTCCA AGGTCTGGTT 
    TATTTGTTGG AGAACGATGT GTCGACTCTG GGCTACGAGC TGACATTCAG CACAGAGGTC 
    CAAGAGTTTG GCGTGTGTGA AGTGAGAGAT CTGAAGGCCA ACGGTGCCAA CATTCTTGTC 
    ACAGAAGAGA ATAAGAAGGA ATATGTGCAT CTGGTCTGCC AGATGAAGAT GACAGGTGCA 
    ATCCGTAAAC AGCTGGCTGC CTTCCTGGAG GGCTTTTATG AGATCATCCC CAAGAGACTG 
    ATCTCCATCT TCACTGAGCA AGAGCTGGAG CTGCTCATCT CCGGCCTGCC CACCATTGAC 
    ATCGACGACC TCAAGGCCAA TACTGAATAC CATAAATATC AGTCCAGTTC TATTCAGATC 
    CAGTGGTTCT GGCGTGCTTT ACGATCCTTC GACCAGGCTG ACAGGGCCAA ATTTCTGCAG 
    TTTGTTACGG GCACTTCCAA GGTGCCACTG CAGGGCTTTG CTGCACTGGA GGGAATGAAC 
    GGCATTCAGA AGTTCCAGAT CCACCGAGAT GACCGCTCCA CTGACCGCCT GCCCTCTGCG 
    CACACTTGCT TTAACCAGTT GGACCTCCCA GCGTACGAGA GCTACGAGAA GTTAAGACAC 
    ATGCTGCTGC TGGCCATTCA GGAATGTTCT GAAGGCTTCG GACTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_001923865.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001923900.2 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa27858
    Clone ID Related Accession (Same CDS sequence) XM_021473709.1 , XM_001923865.5 , XM_001923865.6
    Accession Version XM_001923865.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001923865.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    13501
    13561
    13621
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGCTTCAC TCAGTCCAGA TATAGCTGAA AGTTCAGAGC CTGTGCCAGT GGGTGTGTCT 
    CAACTCGAGG GTTCGCCCAT GGATACGAGC AGTCCCGCTT CAGGAACGCA GGAAGAGCCG 
    GCTGCCAGCT CAGTTCCATC CAATCACATG TCTCAGGAGC TATCAGGAAG TGGTGACAGC 
    GGCCTCACAG ACAGGCAGAC AGATGCAGAA ACTGGATCAA CTTCTGTTTC ATCTCCTGGC 
    GAGACCATGC CTCGGAGTGA CAGTGCCGAT AGCCAGTCGC AGGCCATCCA GGAGGAGCCC 
    CTCCCTTCCA CCAGCAATGA AGAGGAGGAC CCTCTGGCAG GCATCAGTCT ACCTGAAGGC 
    GTGGATCCGT CTTTCTTGGC AGCACTGCCT GAAGACATTC GTCGGGAGGT GCTGCAGAAC 
    CAACTGGGAA TCAGACCTCC TTCTCGACCT CCTGTCGCCT CCACTCTGAC AACCACTGCT 
    AGTGCTGTGC TAGGAGGAGG GCCAGGGGTG ACCGAAGTCA GCCCTGAATT CTTAGCGGCT 
    CTCCCACCTG CAATTCAGGA AGAGGTCCTG GCCCAGCAGC GGGCAGAGCA GCAGCGTCGG 
    GAGTTATCCC AGCAACCCAC ACAGGGTGAT CAACCTCTGG ACCCAGTCAC ATTCATCCAG 
    ACCCTGCCCT CTGAGCTGCG GCGCTCCGTT CTGGAAGACA TGGAGGACAG TGTACTAGCG 
    GTCATGCCTC CAGATATTGC AGCAGAGGCT GCAGCACTGC GAAGGGAACA AGAGGCCAGA 
    CAGAGACAAC TGATGCACGA GCGCTTGTTC GGTCACAGCT CCTCTTCAGC TCTGTCTGCC 
    ATCTTACGCT CTCCCGCCTT CACCAGCCGC CTCGGGGGCA ACCGTGGGGT ACAGTACACC 
    CGGCTGGCTG TGCAAAGAGG AGGGACGTTT CAAATGGGAG GGAGTGCCAA TCATAGCAGA 
    CCCTCTAGTT CAAACGTTGA CTCTCTCCTG CGCCTGCGGG GGCGTCTCTT GTTGGACCAC 
    GAGGCCTTGT CCTGCCTTCT TGTGCTGCTT TTTGTGGACG AGCCCAAACT TAACACCAGC 
    CGCCTCCACC GAGTGCTGCG TAACTTGTGC TATCACTCTC AGACGCGTGG CTGGGTCATC 
    CGCAGTCTCC TGTCCATCCT GCAGCGCAGC AGTGAGAGCG AGCTGTGCCT GGAGACTGCA 
    CGCTTGGAGG AGTCCCGTGG CAAACGCTCA CTAGGCCAGG GCTCATCTAG TGGAGGAAAG 
    GGTTGTTCTT CATCCTCCCA GTCGGCTATA GCCTCTTCGT CATCGTCCTC CTCGTCCTCT 
    TCTTCTCTGG AGCTGCTGAA CAGGGTGGAG TCACGGAGTT CCAGTCAGCT CTCCTGGCTG 
    TCTGTATCAA TGGACGCAGC TCTTGGATGC AGAACCAACA TCTTTCAGAT CCAGCGAGTA 
    TCAGGCAGGA AACACGCAGA GAGGCATTCA GCAGGAGGGA GTGTGAGCGC AGGAGGACTC 
    ACAGGTGGAG TGTCAGGAAC AGGAATGACG TGTACAGGGG GAGGTGGGTC AACTGTTCAC 
    ATCCATCCAC AGGCTGCGCC GGTGGTCTGT CGACATGTAC TGGACACCCT CATCCAATTG 
    GCCAAGGTAT TTCCAAGTCA CTTCACTCAA CAACGCTGTA AAGATCCCCT ATCCTCCTCC 
    TCCTCTGAGT TGGACACTCG TCTTTGTGCC AGCTCAACAC CGACTATGAG TGCCAGTTGT 
    CGGTCAGGTG GCACCAGTCA GAGTAACTCC TCTGCTACCC CAAGTAGTCT CACTTCTATC 
    CACAATTCCC TGAGCGCCTG CTCTGGTACG CCACAGTCCC TAGGCATCTC CACCGACTTT 
    TGGGATCTCC TGGTCAAGCT GGACAACATG AATGTGAGCC GAAAGGGGAA GGCCTCCATG 
    AAGACTGTCC CTCTAGGGGG TGTCGGCGAG ACAGAGGGTG CGCTGTTCAG CCTTGAGGCG 
    TCTCCGCTGG GCCAGCTGAT GAATATGCTG TCCCACCCTG TGATCCGCCG TAGTTCCCTC 
    CTCACGGAGA AACTGCTACG TCTGCTGTCT CTGATCTCCA TCGCTCTGCC TGACAACAAG 
    GCCACGGAGG TTCCAGCCAG CCATCCAGTG CCTCAGACCC CAACTGCCAC GCCATCTGCA 
    CCTGCTCCTC AAACCACAGC AGGGTCAGGG ACCATCTCAG CAGTGGCAGC TACAGGCACT 
    ACGCAGGGTT CAGTCGGTTC AGTGGGTACA ACGCCATCTC AACCAGCCGC TACAATATCT 
    GTGGTTGCAG GCAGCACCAC CGCCTCTAGC ATTATTGCTG GAAAACTGAA GCCAGCAGTA 
    AGCATCACGG ACATTGAATC TAAACTGGCC TCTACTGGAC TTACGGAGAA GCAGCTGCAG 
    CTCTCTGTGG AGGTTCTGAC ATCTCACTCG TGTTCAGAGG AAGGTCTGGA GGACGCAGCC 
    AACATCTTGC TGCAGCTGTC CAGAGGGGAC GCACCGACCC GAGACACTGT GCTTAGACTT 
    CTGCTCAGCG GAGCACGTCA CCTTGGATAC ACTCTCTGCA AACAAATCGG CACTTTATTA 
    GCTGAGTTGA GGGAGTATAA TCTAGAGCAG CAGAGAAGAG CACGGGCTGA TGCTCAGTCT 
    CCTGAAGCTT CTCATGATGA CCCATCCATC ACCACACGCC TCAAGGGCAA GATGACCAGC 
    AGGTTTGACG GGTCAGAGAG TGTGGTGATT GTTGCTGCTC AGAAGCGCAC GTTAGGTGGC 
    CGGGAGCTTC AGTTGCCCTG CATGAGCTCG CTCACTTCCA AGACCTCAAC GCAGAAGTTT 
    TTCTTGCGGG TACTGCAGGT CATCATCCAG CTTAGAGAAG ACACGCGGCG CGCTAACAAG 
    AAAGCCAAGC AGACAGGAGG AAGACTAAGT TCAACCAGTC TGGGCTCGGC CAGTAGTATT 
    CAGGCTGCGG TCCGTCAGCT GGAGGCTGAA GCGGACGCCA TCATTCAGAT GGTGAGAGAG 
    GGACAGAGGG CACGCCGTCT ACAGCAGGCC CCCCCACCCT CTTCATCTGC ATCCTCTTCC 
    GCTGCTGCTG CTGCTGCATC CGCTGCTGTG GCTGTGGCTG GATCGTCTGC CCCCAGTACC 
    TCTGCAACCA ACAACCCCCC CGCTGGCACG GCCAGCGCAG CCACGTCGGA GGTTCACACT 
    GTTGCCGAGC CACCAGCAGC TCAGAGAGAT GATTCGCCCA TGGATGTGGA TCAGCCCTCT 
    CCTTTGGAAC AGGACCAGCC AACATTAGGT GAGGAGAGCA GCAGTCAGCA GGAGCAGGAG 
    GAGCGTTTGC CTGACCTGCC GCTGTTAAGT GAGCAGCTGC TTTTGGATGA GCTGTGGGAC 
    ATGCTTGGAG AATGTCTGAA AGAGCTTGAA GAGTCTCATG ACCAACATGC AGTTCTAGTG 
    CTGCAGCCTG CAGTGGAGGC CTTTTTCTTG GTGCATGCAA CAGAGCGGGA AAGCAAACCA 
    CCAGTTCGGG ACACTCGGGA GAGCCAGCTG TCACACATCA AGGATGAGCC TCCACCCCTG 
    TCACCCGCCC CTCTCACACC CGCTACCCCC TCCTCTCTGG ACCCCTTTTT TTCCAGAGAG 
    CCTTCCTCTA TGCACATCTC TTCCAACCTG CCTCCAGATA CACAGAAGTT CCTCCGTTTC 
    GCAGAGACCC ACCGCACAGT GCTGAATCAG ATCCTGCGTC AATCCACCAC TCACCTGGCA 
    GACGGGCCTT TCGCTGTTTT GGTTGACTAC ATTCGCATCT TGGACTTTGA CGTCAAGCGC 
    AAGTATTTCC GTCAGGAGCT GGAGCGTCTG GATGAAGGCT TGAGGAAAGA GGATATGGCC 
    GTCCATGTGA GGAGAGATCA CGTCTTTGAA GATTCTTACA GAGAGCTGCA CCGCAAGAGC 
    CCAGAAGACA TGAAGAACAG ATTGTATATA GTATTTGAAG GAGAGGAGGG TCAGGATGCA 
    GGCGGGCTGT TAAGAGAGTG GTACATGATC ATCTCCAGAG AAATGTTCAA CCCCATGTAT 
    GCCCTGTTTC GCACCTCCCC CGGCGATCGG GTCACCTATA CCATCAACCC CTCCTCCCAC 
    TGCAACCCCA ACCATCTCAG CTACTTCAAG TTTGTGGGAC GTGTGGTGGC CAAAGCAGTC 
    TACGACAACA GGCTATTAGA GTGTTACTTT ACACGCAGCT TCTACAAGCA CATCCTGGGC 
    AAGAGCGTTA GGTATACAGA CATGGAAAGC GAAGACTATC CCTTTTTCCA AGGTCTGGTT 
    TATTTGTTGG AGAACGATGT GTCGACTCTG GGCTACGAGC TGACATTCAG CACAGAGGTC 
    CAAGAGTTTG GCGTGTGTGA AGTGAGAGAT CTGAAGGCCA ACGGTGCCAA CATTCTTGTC 
    ACAGAAGAGA ATAAGAAGGA ATATGTGCAT CTGGTCTGCC AGATGAAGAT GACAGGTGCA 
    ATCCGTAAAC AGCTGGCTGC CTTCCTGGAG GGCTTTTATG AGATCATCCC CAAGAGACTG 
    ATCTCCATCT TCACTGAGCA AGAGCTGGAG CTGCTCATCT CCGGCCTGCC CACCATTGAC 
    ATCGACGACC TCAAGGCCAA TACTGAATAC CATAAATATC AGTCCAGTTC TATTCAGATC 
    CAGTGGTTCT GGCGTGCTTT ACGATCCTTC GACCAGGCTG ACAGGGCCAA ATTTCTGCAG 
    TTTGTTACGG GCACTTCCAA GGTGCCACTG CAGGGCTTTG CTGCACTGGA GGGAATGAAC 
    GGCATTCAGA AGTTCCAGAT CCACCGAGAT GACCGCTCCA CTGACCGCCT GCCCTCTGCG 
    CACACTTGCT TTAACCAGTT GGACCTCCCA GCGTACGAGA GCTACGAGAA GTTAAGACAC 
    ATGCTGCTGC TGGCCATTCA GGAATGTTCT GAAGGCTTCG GACTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001923900.2
    CDS142..13812
    Translation

    Target ORF information:

    RefSeq Version XM_001923865.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001923865.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    13621
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGCTTCAC TCAGTCCAGA TATAGCTGAA AGTTCAGAGC CTGTGCCAGT GGGTGTGTCT 
    CAACTCGAGG GTTCGCCCAT GGATACGAGC AGTCCCGCTT CAGGAACGCA GGAAGAGCCG 
    GCTGCCAGCT CAGTTCCATC CAATCACATG TCTCAGGAGC TATCAGGAAG TGGTGACAGC 
    GGCCTCACAG ACAGGCAGAC AGATGCAGAA ACTGGATCAA CTTCTGTTTC ATCTCCTGGC 
    GAGACCATGC CTCGGAGTGA CAGTGCCGAT AGCCAGTCGC AGGCCATCCA GGAGGAGCCC 
    CTCCCTTCCA CCAGCAATGA AGAGGAGGAC CCTCTGGCAG GCATCAGTCT ACCTGAAGGC 
    GTGGATCCGT CTTTCTTGGC AGCACTGCCT GAAGACATTC GTCGGGAGGT GCTGCAGAAC 
    CAACTGGGAA TCAGACCTCC TTCTCGACCT CCTGTCGCCT CCACTCTGAC AACCACTGCT 
    AGTGCTGTGC TAGGAGGAGG GCCAGGGGTG ACCGAAGTCA GCCCTGAATT CTTAGCGGCT 
    CTCCCACCTG CAATTCAGGA AGAGGTCCTG GCCCAGCAGC GGGCAGAGCA GCAGCGTCGG 
    GAGTTATCCC AGCAACCCAC ACAGGGTGAT CAACCTCTGG ACCCAGTCAC ATTCATCCAG 
    ACCCTGCCCT CTGAGCTGCG GCGCTCCGTT CTGGAAGACA TGGAGGACAG TGTACTAGCG 
    GTCATGCCTC CAGATATTGC AGCAGAGGCT GCAGCACTGC GAAGGGAACA AGAGGCCAGA 
    CAGAGACAAC TGATGCACGA GCGCTTGTTC GGTCACAGCT CCTCTTCAGC TCTGTCTGCC 
    ATCTTACGCT CTCCCGCCTT CACCAGCCGC CTCGGGGGCA ACCGTGGGGT ACAGTACACC 
    CGGCTGGCTG TGCAAAGAGG AGGGACGTTT CAAATGGGAG GGAGTGCCAA TCATAGCAGA 
    CCCTCTAGTT CAAACGTTGA CTCTCTCCTG CGCCTGCGGG GGCGTCTCTT GTTGGACCAC 
    GAGGCCTTGT CCTGCCTTCT TGTGCTGCTT TTTGTGGACG AGCCCAAACT TAACACCAGC 
    CGCCTCCACC GAGTGCTGCG TAACTTGTGC TATCACTCTC AGACGCGTGG CTGGGTCATC 
    CGCAGTCTCC TGTCCATCCT GCAGCGCAGC AGTGAGAGCG AGCTGTGCCT GGAGACTGCA 
    CGCTTGGAGG AGTCCCGTGG CAAACGCTCA CTAGGCCAGG GCTCATCTAG TGGAGGAAAG 
    GGTTGTTCTT CATCCTCCCA GTCGGCTATA GCCTCTTCGT CATCGTCCTC CTCGTCCTCT 
    TCTTCTCTGG AGCTGCTGAA CAGGGTGGAG TCACGGAGTT CCAGTCAGCT CTCCTGGCTG 
    TCTGTATCAA TGGACGCAGC TCTTGGATGC AGAACCAACA TCTTTCAGAT CCAGCGAGTA 
    TCAGGCAGGA AACACGCAGA GAGGCATTCA GCAGGAGGGA GTGTGAGCGC AGGAGGACTC 
    ACAGGTGGAG TGTCAGGAAC AGGAATGACG TGTACAGGGG GAGGTGGGTC AACTGTTCAC 
    ATCCATCCAC AGGCTGCGCC GGTGGTCTGT CGACATGTAC TGGACACCCT CATCCAATTG 
    GCCAAGGTAT TTCCAAGTCA CTTCACTCAA CAACGCTGTA AAGATCCCCT ATCCTCCTCC 
    TCCTCTGAGT TGGACACTCG TCTTTGTGCC AGCTCAACAC CGACTATGAG TGCCAGTTGT 
    CGGTCAGGTG GCACCAGTCA GAGTAACTCC TCTGCTACCC CAAGTAGTCT CACTTCTATC 
    CACAATTCCC TGAGCGCCTG CTCTGGTACG CCACAGTCCC TAGGCATCTC CACCGACTTT 
    TGGGATCTCC TGGTCAAGCT GGACAACATG AATGTGAGCC GAAAGGGGAA GGCCTCCATG 
    AAGACTGTCC CTCTAGGGGG TGTCGGCGAG ACAGAGGGTG CGCTGTTCAG CCTTGAGGCG 
    TCTCCGCTGG GCCAGCTGAT GAATATGCTG TCCCACCCTG TGATCCGCCG TAGTTCCCTC 
    CTCACGGAGA AACTGCTACG TCTGCTGTCT CTGATCTCCA TCGCTCTGCC TGACAACAAG 
    GCCACGGAGG TTCCAGCCAG CCATCCAGTG CCTCAGACCC CAACTGCCAC GCCATCTGCA 
    CCTGCTCCTC AAACCACAGC AGGGTCAGGG ACCATCTCAG CAGTGGCAGC TACAGGCACT 
    ACGCAGGGTT CAGTCGGTTC AGTGGGTACA ACGCCATCTC AACCAGCCGC TACAATATCT 
    GTGGTTGCAG GCAGCACCAC CGCCTCTAGC ATTATTGCTG GAAAACTGAA GCCAGCAGTA 
    AGCATCACGG ACATTGAATC TAAACTGGCC TCTACTGGAC TTACGGAGAA GCAGCTGCAG 
    CTCTCTGTGG AGGTTCTGAC ATCTCACTCG TGTTCAGAGG AAGGTCTGGA GGACGCAGCC 
    AACATCTTGC TGCAGCTGTC CAGAGGGGAC GCACCGACCC GAGACACTGT GCTTAGACTT 
    CTGCTCAGCG GAGCACGTCA CCTTGGATAC ACTCTCTGCA AACAAATCGG CACTTTATTA 
    GCTGAGTTGA GGGAGTATAA TCTAGAGCAG CAGAGAAGAG CACGGGCTGA TGCTCAGTCT 
    CCTGAAGCTT CTCATGATGA CCCATCCATC ACCACACGCC TCAAGGGCAA GATGACCAGC 
    AGGTTTGACG GGTCAGAGAG TGTGGTGATT GTTGCTGCTC AGAAGCGCAC GTTAGGTGGC 
    CGGGAGCTTC AGTTGCCCTG CATGAGCTCG CTCACTTCCA AGACCTCAAC GCAGAAGTTT 
    TTCTTGCGGG TACTGCAGGT CATCATCCAG CTTAGAGAAG ACACGCGGCG CGCTAACAAG 
    AAAGCCAAGC AGACAGGAGG AAGACTAAGT TCAACCAGTC TGGGCTCGGC CAGTAGTATT 
    CAGGCTGCGG TCCGTCAGCT GGAGGCTGAA GCGGACGCCA TCATTCAGAT GGTGAGAGAG 
    GGACAGAGGG CACGCCGTCT ACAGCAGGCC CCCCCACCCT CTTCATCTGC ATCCTCTTCC 
    GCTGCTGCTG CTGCTGCATC CGCTGCTGTG GCTGTGGCTG GATCGTCTGC CCCCAGTACC 
    TCTGCAACCA ACAACCCCCC CGCTGGCACG GCCAGCGCAG CCACGTCGGA GGTTCACACT 
    GTTGCCGAGC CACCAGCAGC TCAGAGAGAT GATTCGCCCA TGGATGTGGA TCAGCCCTCT 
    CCTTTGGAAC AGGACCAGCC AACATTAGGT GAGGAGAGCA GCAGTCAGCA GGAGCAGGAG 
    GAGCGTTTGC CTGACCTGCC GCTGTTAAGT GAGCAGCTGC TTTTGGATGA GCTGTGGGAC 
    ATGCTTGGAG AATGTCTGAA AGAGCTTGAA GAGTCTCATG ACCAACATGC AGTTCTAGTG 
    CTGCAGCCTG CAGTGGAGGC CTTTTTCTTG GTGCATGCAA CAGAGCGGGA AAGCAAACCA 
    CCAGTTCGGG ACACTCGGGA GAGCCAGCTG TCACACATCA AGGATGAGCC TCCACCCCTG 
    TCACCCGCCC CTCTCACACC CGCTACCCCC TCCTCTCTGG ACCCCTTTTT TTCCAGAGAG 
    CCTTCCTCTA TGCACATCTC TTCCAACCTG CCTCCAGATA CACAGAAGTT CCTCCGTTTC 
    GCAGAGACCC ACCGCACAGT GCTGAATCAG ATCCTGCGTC AATCCACCAC TCACCTGGCA 
    GACGGGCCTT TCGCTGTTTT GGTTGACTAC ATTCGCATCT TGGACTTTGA CGTCAAGCGC 
    AAGTATTTCC GTCAGGAGCT GGAGCGTCTG GATGAAGGCT TGAGGAAAGA GGATATGGCC 
    GTCCATGTGA GGAGAGATCA CGTCTTTGAA GATTCTTACA GAGAGCTGCA CCGCAAGAGC 
    CCAGAAGACA TGAAGAACAG ATTGTATATA GTATTTGAAG GAGAGGAGGG TCAGGATGCA 
    GGCGGGCTGT TAAGAGAGTG GTACATGATC ATCTCCAGAG AAATGTTCAA CCCCATGTAT 
    GCCCTGTTTC GCACCTCCCC CGGCGATCGG GTCACCTATA CCATCAACCC CTCCTCCCAC 
    TGCAACCCCA ACCATCTCAG CTACTTCAAG TTTGTGGGAC GTGTGGTGGC CAAAGCAGTC 
    TACGACAACA GGCTATTAGA GTGTTACTTT ACACGCAGCT TCTACAAGCA CATCCTGGGC 
    AAGAGCGTTA GGTATACAGA CATGGAAAGC GAAGACTATC CCTTTTTCCA AGGTCTGGTT 
    TATTTGTTGG AGAACGATGT GTCGACTCTG GGCTACGAGC TGACATTCAG CACAGAGGTC 
    CAAGAGTTTG GCGTGTGTGA AGTGAGAGAT CTGAAGGCCA ACGGTGCCAA CATTCTTGTC 
    ACAGAAGAGA ATAAGAAGGA ATATGTGCAT CTGGTCTGCC AGATGAAGAT GACAGGTGCA 
    ATCCGTAAAC AGCTGGCTGC CTTCCTGGAG GGCTTTTATG AGATCATCCC CAAGAGACTG 
    ATCTCCATCT TCACTGAGCA AGAGCTGGAG CTGCTCATCT CCGGCCTGCC CACCATTGAC 
    ATCGACGACC TCAAGGCCAA TACTGAATAC CATAAATATC AGTCCAGTTC TATTCAGATC 
    CAGTGGTTCT GGCGTGCTTT ACGATCCTTC GACCAGGCTG ACAGGGCCAA ATTTCTGCAG 
    TTTGTTACGG GCACTTCCAA GGTGCCACTG CAGGGCTTTG CTGCACTGGA GGGAATGAAC 
    GGCATTCAGA AGTTCCAGAT CCACCGAGAT GACCGCTCCA CTGACCGCCT GCCCTCTGCG 
    CACACTTGCT TTAACCAGTT GGACCTCCCA GCGTACGAGA GCTACGAGAA GTTAAGACAC 
    ATGCTGCTGC TGGCCATTCA GGAATGTTCT GAAGGCTTCG GACTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_005162282.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162339.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID ODa27859
    Clone ID Related Accession (Same CDS sequence) XM_005162282.3 , XM_021473710.1 , XM_005162282.4
    Accession Version XM_005162282.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162282.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    1381
    1441
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    1981
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    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAAGCAGCT 
    CGCAGGCGTG AGGAGGAGGA GAGGAGAGCG AGGGAGCGGG TAGAGGAGGA AGAAGCTCGT 
    TGTCTGGAGC GCTTTCTGGA AGCAGAGCCT CTTGACACCA CTGAGCTTCA TGCCTTCACA 
    GACTCCATGC TGCCTGGCTG CTTCCACCTG CTCGATGAGC TGCCGGACAC AGTCTACCGC 
    CTCTGTGATC TGCTCATGAC TGCTATTAAA AGAAACGGCC CTGAATACAG AGACCTCATC 
    CTTAGACAAG TGGTCAATCA GGTGTGGGAG GCTGCTGACG TGTTGATAAA GGCTGCAGTG 
    CCGCTAACCA CCAGCGACAC CAAGACAGTC TCTGAGTGGA CCAGACAGAT GGCGACCCTC 
    CCTCAGGCCT CCAACCTGGC CACCCGCATC CTGCTGCTCA CGCTGCTCTT TGAGGAGCTG 
    AAACTCTCCT GCGCTCGGGT GGTGGAGAGC AGTGGTGTCT TGACTGTGCT GATTAAGCTG 
    TTGGAGGTGG TGCAGCCCTG TCTGCAAGCT GCCAAAGAGC AGAAGGACAT CCAAACGCCC 
    AAATGGATCA CCCCTGTGCT CCTACTTATT GACTTTTATG AGAAAATGGC TGTTTCATCC 
    AAACGAAGAG CTCAGATGAA CAAGTACCTC CAGCCCAATG GGAATAACTG GCGCTGGTTT 
    GATGACCGTT CAGGCCGCTG GTGCAGCTAC AGTGCAAGTA ACAACAGCAC TATTGACTCT 
    GCATGGAGGG CAGGAGAGAC CAGTGTCCGA TTTACAGCTG GCCGCAGAAG ATACACAGTT 
    CAGTTCAACA CCATGGTGCA GGTAAATGAG GAGACTGGCA ACAGACGACC AGTTATGTTG 
    ACGGTACAGC GGGTGCCACG CATTCCCAAG GCAACAAAAT CCGGAAGTGT TACTGACACG 
    GAGAGAGAGG AGAGTGAACA GAGAGCAAAG ACTGAGGAAA CACAGACTGA CACAGATTCC 
    AGCTCTGTGG CTGTAGAGAT GGCGCCCCCT AAAGAAGAAT CTGAGCTGAA GGATTCAACC 
    CCAGCAGCTC CGTCAGTTCT CCAGGACCCC TCTGGTTCTA GTGGTATCGT TGTGCAGGGG 
    CTGACAGAAG ACATGATCAC CATTTTAATC CGTGCCTGTG TCAGCATGAT CAGTGTTCCT 
    GTGGACCCAG ACACTTTGCA CGCCACCTTG CGGCTCTGTC TGCGCCTCAC ACGCACACAC 
    CATTACGCCA TGATGTTTGC AGAGCTCAAG AGCACCCGCA TGATTCTGAA TCTCACCCAG 
    AGCTCAGGCT TTAATGGCTT CACCCCATTG GTCACCCTGC TCTTCCGCCA CATCATAGAG 
    GACCCGGCCA CACTGCGGCA CACTATGGAG AAGGTGGTAC GTTCTGCAGT CACCAGTGGT 
    GCAGGAAGCA CTACTTCAGG TGTGGTGTCA GGTAGTCTGG GCTCCAGGGA AATTAACTAC 
    ATCCTGCGAG TCCTTGGCCC AGCGGCCTGT CGCAATCCAG AGTGCTTTAC GGAAACTGCC 
    AAGAGTTGCA TCCGAATTGC TCTCCCGGCT CCAAGAGGTG CTGGCACTGC CTCAGATGAC 
    GAGTTTGAGA ACTTCAGAAT TAAAGGTCCA AATGCTGTGC AACTCGTGAA GACCACCCCC 
    CTAAAGCTCT CCCCGTTGCC CTCCATACCA GAGACTATAA AAGAGGTCAT CTATGACATG 
    CTGAATGCCC TGGCAGCATA TCACGCACCA GAAGAGGCAG AGAGAGGAGA GGAGCGGGCA 
    AACGCTGTAC CAGGGAATCA AGACCTGTGT CAGATCCTGC AGGATGTCGG TGATGATGTC 
    TACCAGCAGT ACAGACTCAC CAGACAAGGC AGTGATTTTG ACTCGCAATC TGCCTTCCCC 
    ATCAATACTC AGGTGTTTGC TGCAGATGGT GCTGTAGCTG AATCTTCTCA GTCTGGAACT 
    CCACAGGGAG AAGCCTCTAC ACCCGAGGAA ATGCGTGAAG AGAAAAAGGA GCAGGAAGGA 
    GACAAGGGAG CTTCTTCGGA GGAGAGCCGA GCTGCTAAGG CTAAAGCTAG CAAACCTCTG 
    ATGCCCACCT CTACCATCTT GAGGCTGTTG GCCGAGCTGG TTCGCTCTTA TGTTGGCATT 
    GCCACTCTTA TCACCTCTTA CAACTACACA GCTGGCCAGT CGGAGCTCAT TAAGGAAGAT 
    TGCAGTGTGT TGGCATTTGT TTTGGACCAC TTGCTCCCAC ACACCCAGAA CTCTGAAGAT 
    AAGGACACCC CTGCCCTTGC ACGCCTTTTC TTGGCCAGCC TTGCTGCAGC TGGCACGGGC 
    ACCGATGCCC AGGCTGCACT GGTCAACGAG GTCAAAGCTG CTTTGAGTCG AGCATTGGGC 
    ATGGCAGAGG GCACAGAGAA ACACGCCCGT TTGCAAGCAG TGATGTGCAT CATCAGCACC 
    ATTATGGAGT CGTGTCCTTC CACCTCCAGC TTCTACAGCA CAGCCACAGC TAAGACCCAG 
    CACAACGGCA TGAACAATAT CATCCGGCTC TTCTTGAAGA AAGGCCTTGT CAATGACCTG 
    GCCCGTGTGC CTCACAGCCT TGATCTATCC AGTCCCAATA TGGCAAACAC AGTAAATGCT 
    GCTTTGAAGC CTCTGGAAAC ATTGTCGCGA ATTGTGAACC AGCCCAGCAG CCTGTTTGGT 
    GGTAAAGGGG GATCCAGCAA GAACAAGACT GAACATGACA CTGTTGGCAC AGTCAGAGAC 
    AGTAACAGCA ACACTCAGGA TCAAGGAGAA GTGGGTGAGG CAGAGCCGGT TGAGAATCGT 
    GACCGAGGTC AAGCCACAGA CTCTGACCTG ATGGATGGAG AAGCCGAGGG AGACACTGTG 
    GTCATTGCTG GACAGCCTGA GGTGCTGAGC ACTCAGGCAA TGCAGGTGGA GAACGAGTTG 
    GTGGATTTGA TCGATGAACT TTTAGAGAGA GATGCCCAAA CTGTTAATAG TACCATCATC 
    GTAGGTCGTT CTGGAGAGGA TGAGTCTCAA GAGGATGTGC TGATGGATGA AGCTCCTTCC 
    AATATGAGTC AGGCCTCCAC TCTCCAAGCC AACAGAGAAG ACTCCATGAA CATCCTCGAG 
    CCAGAAGATG AGGAGCACAC GCAGGAGGAA GACTCCAGTG GTAGCAACGA GGATGAGGAC 
    AGTCAGGATG AAGAGGAAGA GGAGGAGGAA GAAGAGGAGG AAGATCAGGA TGATGAAGAG 
    GGAGATGAGG ATGATGACGA TGAAGGCTCA GAGATGGAAC TGGATGAAGA TTTTCCAGAT 
    ATCAATGCTG CCCCTCATAT TCGTTTTGAG CGATTCGACC GAGATGATGA CCTCATCATT 
    GAGTTTGATA ATATGTTCTC CACTTCAGCT GACATCCCTC CCTCACCAGG AAATATCCCC 
    AGCTCCCACC CTCTGATGGT GCGTCACGCT GACCATGGCT CGCTGACTCT TGGGGGATCG 
    GGCACCAGTA GCAGATTGGC TCAAGGAATG GGACGTAGTC AACGTTCTCT CCGCCAGCTC 
    ACGGCCAACA CAGGCCACAC GATCCATGTA CATTATCCTG GCAACCGTCA GCCTAACCCT 
    CCACTCATTC TGCAGAGATT ATTGGGCCCC AGCGCTGCAG CTGATATCTT GCAGCTGTCC 
    AGCTCTCTTC CTCTGCAGTC TCGAGGTCGT GCCCGTTTAA TGGTGGGTAA TGAAGATGTG 
    CACATTATTG CCCGTTCAGA CGATGAGCTG CTGGATGACT TCTTCCATGA GCAGAGCAGC 
    ACCGGAGGAC AAGCTGGCAC CCTTTCAAGT ATCCCGACTG CCTTAACCAG ATGGACAGAT 
    GAGTGTAAAG TGCTGGATGC TGAGAGCATG CATGACTGTG TGGCAGTGGT GAAGGTGCCC 
    ATACTGCAGC ACCTGGAGAG TTTGCGTGAT GAGGAGCTGG AGGAACGCAG GGAGAAAAGG 
    AGGAGGCAGT TGGCAGAGGA AGAGGAGACT GCAAAGCAGA ATGAGAGTTC TGCAGCAGGG 
    GAGGAGACCA GGGAACAGAG TCTGCAGGGC TCTGGGATTG GAACAGTCAA TGGAGCTGCA 
    GAGAATGCTG CAGAAGGAGA ACAGGCCCAG AGTGGGGTGT CCTCCTGTCT GGACCCTCCT 
    CGCACCTCTG AGGGTTTCCT CACAGCGCCC CCATCAGGAG AAGTCACTCC AACCACCCCT 
    GCCCCCCATG AACAAGCCCT CGTGTCTCTG GAAACAGCCA TCAGTCAGCA GGTGCACCAG 
    CCAATCACAG AGCTGCTGCT GGCCGACTCC AATCCTGACT CTCACGGAGC CTTGGCAGGG 
    GCAGGGCTTC CCCAGCTCTC AGCACCTGAT AGGCCAAATT GTGAGGCAGA AGCATCTCAG 
    ATGGAGATGT CACCTGCACC CACTATTGGT GAGAGAGGAG GAGGAGCATT GGATGCAGTC 
    AGGGAAGCTT CACTCAGTCC AGATATAGCT GAAAGTTCAG AGCCTGTGCC AGTGGGTGTG 
    TCTCAACTCG AGGGTTCGCC CATGGATACG AGCAGTCCCG CTTCAGGAAC GCAGGAAGAG 
    CCGGCTGCCA GCTCAGTTCC ATCCAATCAC ATGTCTCAGG AGCTATCAGG AAGTGGTGAC 
    AGCGGCCTCA CAGACAGGCA GACAGATGCA GAAACTGGAT CAACTTCTGT TTCATCTCCT 
    GGCGAGACCA TGCCTCGGAG TGACAGTGCC GATAGCCAGT CGCAGGCCAT CCAGGAGGAG 
    CCCCTCCCTT CCACCAGCAA TGAAGAGGAG GACCCTCTGG CAGGCATCAG TCTACCTGAA 
    GGCGTGGATC CGTCTTTCTT GGCAGCACTG CCTGAAGACA TTCGTCGGGA GGTGCTGCAG 
    AACCAACTGG GAATCAGACC TCCTTCTCGA CCTCCTGTCG CCTCCACTCT GACAACCACT 
    GCTAGTGCTG TGCTAGGAGG AGGGCCAGGG GTGACCGAAG TCAGCCCTGA ATTCTTAGCG 
    GCTCTCCCAC CTGCAATTCA GGAAGAGGTC CTGGCCCAGC AGCGGGCAGA GCAGCAGCGT 
    CGGGAGTTAT CCCAGCAACC CACACAGGGT GATCAACCTC TGGACCCAGT CACATTCATC 
    CAGACCCTGC CCTCTGAGCT GCGGCGCTCC GTTCTGGAAG ACATGGAGGA CAGTGTACTA 
    GCGGTCATGC CTCCAGATAT TGCAGCAGAG GCTGCAGCAC TGCGAAGGGA ACAAGAGGCC 
    AGACAGAGAC AACTGATGCA CGAGCGCTTG TTCGGTCACA GCTCCTCTTC AGCTCTGTCT 
    GCCATCTTAC GCTCTCCCGC CTTCACCAGC CGCCTCGGGG GCAACCGTGG GGTACAGTAC 
    ACCCGGCTGG CTGTGCAAAG AGGAGGGACG TTTCAAATGG GAGGGAGTGC CAATCATAGC 
    AGACCCTCTA GTTCAAACGT TGACTCTCTC CTGCGCCTGC GGGGGCGTCT CTTGTTGGAC 
    CACGAGGCCT TGTCCTGCCT TCTTGTGCTG CTTTTTGTGG ACGAGCCCAA ACTTAACACC 
    AGCCGCCTCC ACCGAGTGCT GCGTAACTTG TGCTATCACT CTCAGACGCG TGGCTGGGTC 
    ATCCGCAGTC TCCTGTCCAT CCTGCAGCGC AGCAGTGAGA GCGAGCTGTG CCTGGAGACT 
    GCACGCTTGG AGGAGTCCCG TGGCAAACGC TCACTAGGCC AGGGCTCATC TAGTGGAGGA 
    AAGGGTTGTT CTTCATCCTC CCAGTCGGCT ATAGCCTCTT CGTCATCGTC CTCCTCGTCC 
    TCTTCTTCTC TGGAGCTGCT GAACAGGGTG GAGTCACGGA GTTCCAGTCA GCTCTCCTGG 
    CTGTCTGTAT CAATGGACGC AGCTCTTGGA TGCAGAACCA ACATCTTTCA GATCCAGCGA 
    GTATCAGGCA GGAAACACGC AGAGAGGCAT TCAGCAGGAG GGAGTGTGAG CGCAGGAGGA 
    CTCACAGGTG GAGTGTCAGG AACAGGAATG ACGTGTACAG GGGGAGGTGG GTCAACTGTT 
    CACATCCATC CACAGGCTGC GCCGGTGGTC TGTCGACATG TACTGGACAC CCTCATCCAA 
    TTGGCCAAGG TATTTCCAAG TCACTTCACT CAACAACGCT GTAAAGATCC CCTATCCTCC 
    TCCTCCTCTG AGTTGGACAC TCGTCTTTGT GCCAGCTCAA CACCGACTAT GAGTGCCAGT 
    TGTCGGTCAG GTGGCACCAG TCAGAGTAAC TCCTCTGCTA CCCCAAGTAG TCTCACTTCT 
    ATCCACAATT CCCTGAGCGC CTGCTCTGGT ACGCCACAGT CCCTAGGCAT CTCCACCGAC 
    TTTTGGGATC TCCTGGTCAA GCTGGACAAC ATGAATGTGA GCCGAAAGGG GAAGGCCTCC 
    ATGAAGACTG TCCCTCTAGG GGGTGTCGGC GAGACAGAGG GTGCGCTGTT CAGCCTTGAG 
    GCGTCTCCGC TGGGCCAGCT GATGAATATG CTGTCCCACC CTGTGATCCG CCGTAGTTCC 
    CTCCTCACGG AGAAACTGCT ACGTCTGCTG TCTCTGATCT CCATCGCTCT GCCTGACAAC 
    AAGGCCACGG AGGTTCCAGC CAGCCATCCA GTGCCTCAGA CCCCAACTGC CACGCCATCT 
    GCACCTGCTC CTCAAACCAC AGCAGGGTCA GGGACCATCT CAGCAGTGGC AGCTACAGGC 
    ACTACGCAGG GTTCAGTCGG TTCAGTGGGT ACAACGCCAT CTCAACCAGC CGCTACAATA 
    TCTGTGGTTG CAGGCAGCAC CACCGCCTCT AGCATTATTG CTGGAAAACT GAAGCCAGCA 
    GTAAGCATCA CGGACATTGA ATCTAAACTG GCCTCTACTG GACTTACGGA GAAGCAGCTG 
    CAGCTCTCTG TGGAGGTTCT GACATCTCAC TCGTGTTCAG AGGAAGGTCT GGAGGACGCA 
    GCCAACATCT TGCTGCAGCT GTCCAGAGGG GACGCACCGA CCCGAGACAC TGTGCTTAGA 
    CTTCTGCTCA GCGGAGCACG TCACCTTGGA TACACTCTCT GCAAACAAAT CGGCACTTTA 
    TTAGCTGAGT TGAGGGAGTA TAATCTAGAG CAGCAGAGAA GAGCACGGGC TGATGCTCAG 
    TCTCCTGAAG CTTCTCATGA TGACCCATCC ATCACCACAC GCCTCAAGGG CAAGATGACC 
    AGCAGGTTTG ACGGGTCAGA GAGTGTGGTG ATTGTTGCTG CTCAGAAGCG CACGTTAGGT 
    GGCCGGGAGC TTCAGTTGCC CTGCATGAGC TCGCTCACTT CCAAGACCTC AACGCAGAAG 
    TTTTTCTTGC GGGTACTGCA GGTCATCATC CAGCTTAGAG AAGACACGCG GCGCGCTAAC 
    AAGAAAGCCA AGCAGACAGG AGGAAGACTA AGTTCAACCA GTCTGGGCTC GGCCAGTAGT 
    ATTCAGGCTG CGGTCCGTCA GCTGGAGGCT GAAGCGGACG CCATCATTCA GATGGTGAGA 
    GAGGGACAGA GGGCACGCCG TCTACAGCAG GCCCCCCCAC CCTCTTCATC TGCATCCTCT 
    TCCGCTGCTG CTGCTGCTGC ATCCGCTGCT GTGGCTGTGG CTGGATCGTC TGCCCCCAGT 
    ACCTCTGCAA CCAACAACCC CCCCGCTGGC ACGGCCAGCG CAGCCACGTC GGAGGTTCAC 
    ACTGTTGCCG AGCCACCAGC AGCTCAGAGA GATGATTCGC CCATGGATGT GGATCAGCCC 
    TCTCCTTTGG AACAGGACCA GCCAACATTA GGTGAGGAGA GCAGCAGTCA GCAGGAGCAG 
    GAGGAGCGTT TGCCTGACCT GCCGCTGTTA AGTGAGCAGC TGCTTTTGGA TGAGCTGTGG 
    GACATGCTTG GAGAATGTCT GAAAGAGCTT GAAGAGTCTC ATGACCAACA TGCAGTTCTA 
    GTGCTGCAGC CTGCAGTGGA GGCCTTTTTC TTGGTGCATG CAACAGAGCG GGAAAGCAAA 
    CCACCAGTTC GGGACACTCG GGAGAGCCAG CTGTCACACA TCAAGGATGA GCCTCCACCC 
    CTGTCACCCG CCCCTCTCAC ACCCGCTACC CCCTCCTCTC TGGACCCCTT TTTTTCCAGA 
    GAGCCTTCCT CTATGCACAT CTCTTCCAAC CTGCCTCCAG ATACACAGAA GTTCCTCCGT 
    TTCGCAGAGA CCCACCGCAC AGTGCTGAAT CAGATCCTGC GTCAATCCAC CACTCACCTG 
    GCAGACGGGC CTTTCGCTGT TTTGGTTGAC TACATTCGCA TCTTGGACTT TGACGTCAAG 
    CGCAAGTATT TCCGTCAGGA GCTGGAGCGT CTGGATGAAG GCTTGAGGAA AGAGGATATG 
    GCCGTCCATG TGAGGAGAGA TCACGTCTTT GAAGATTCTT ACAGAGAGCT GCACCGCAAG 
    AGCCCAGAAG ACATGAAGAA CAGATTGTAT ATAGTATTTG AAGGAGAGGA GGGTCAGGAT 
    GCAGGCGGGC TGTTAAGAGA GTGGTACATG ATCATCTCCA GAGAAATGTT CAACCCCATG 
    TATGCCCTGT TTCGCACCTC CCCCGGCGAT CGGGTCACCT ATACCATCAA CCCCTCCTCC 
    CACTGCAACC CCAACCATCT CAGCTACTTC AAGTTTGTGG GACGTGTGGT GGCCAAAGCA 
    GTCTACGACA ACAGGCTATT AGAGTGTTAC TTTACACGCA GCTTCTACAA GCACATCCTG 
    GGCAAGAGCG TTAGGTATAC AGACATGGAA AGCGAAGACT ATCCCTTTTT CCAAGGTCTG 
    GTTTATTTGT TGGAGAACGA TGTGTCGACT CTGGGCTACG AGCTGACATT CAGCACAGAG 
    GTCCAAGAGT TTGGCGTGTG TGAAGTGAGA GATCTGAAGG CCAACGGTGC CAACATTCTT 
    GTCACAGAAG AGAATAAGAA GGAATATGTG CATCTGGTCT GCCAGATGAA GATGACAGGT 
    GCAATCCGTA AACAGCTGGC TGCCTTCCTG GAGGGCTTTT ATGAGATCAT CCCCAAGAGA 
    CTGATCTCCA TCTTCACTGA GCAAGAGCTG GAGCTGCTCA TCTCCGGCCT GCCCACCATT 
    GACATCGACG ACCTCAAGGC CAATACTGAA TACCATAAAT ATCAGTCCAG TTCTATTCAG 
    ATCCAGTGGT TCTGGCGTGC TTTACGATCC TTCGACCAGG CTGACAGGGC CAAATTTCTG 
    CAGTTTGTTA CGGGCACTTC CAAGGTGCCA CTGCAGGGCT TTGCTGCACT GGAGGGAATG 
    AACGGCATTC AGAAGTTCCA GATCCACCGA GATGACCGCT CCACTGACCG CCTGCCCTCT 
    GCGCACACTT GCTTTAACCA GTTGGACCTC CCAGCGTACG AGAGCTACGA GAAGTTAAGA 
    CACATGCTGC TGCTGGCCAT TCAGGAATGT TCTGAAGGCT TCGGACTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162339.1
    CDS149..13762
    Translation

    Target ORF information:

    RefSeq Version XM_005162282.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005162282.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAAGCAGCT 
    CGCAGGCGTG AGGAGGAGGA GAGGAGAGCG AGGGAGCGGG TAGAGGAGGA AGAAGCTCGT 
    TGTCTGGAGC GCTTTCTGGA AGCAGAGCCT CTTGACACCA CTGAGCTTCA TGCCTTCACA 
    GACTCCATGC TGCCTGGCTG CTTCCACCTG CTCGATGAGC TGCCGGACAC AGTCTACCGC 
    CTCTGTGATC TGCTCATGAC TGCTATTAAA AGAAACGGCC CTGAATACAG AGACCTCATC 
    CTTAGACAAG TGGTCAATCA GGTGTGGGAG GCTGCTGACG TGTTGATAAA GGCTGCAGTG 
    CCGCTAACCA CCAGCGACAC CAAGACAGTC TCTGAGTGGA CCAGACAGAT GGCGACCCTC 
    CCTCAGGCCT CCAACCTGGC CACCCGCATC CTGCTGCTCA CGCTGCTCTT TGAGGAGCTG 
    AAACTCTCCT GCGCTCGGGT GGTGGAGAGC AGTGGTGTCT TGACTGTGCT GATTAAGCTG 
    TTGGAGGTGG TGCAGCCCTG TCTGCAAGCT GCCAAAGAGC AGAAGGACAT CCAAACGCCC 
    AAATGGATCA CCCCTGTGCT CCTACTTATT GACTTTTATG AGAAAATGGC TGTTTCATCC 
    AAACGAAGAG CTCAGATGAA CAAGTACCTC CAGCCCAATG GGAATAACTG GCGCTGGTTT 
    GATGACCGTT CAGGCCGCTG GTGCAGCTAC AGTGCAAGTA ACAACAGCAC TATTGACTCT 
    GCATGGAGGG CAGGAGAGAC CAGTGTCCGA TTTACAGCTG GCCGCAGAAG ATACACAGTT 
    CAGTTCAACA CCATGGTGCA GGTAAATGAG GAGACTGGCA ACAGACGACC AGTTATGTTG 
    ACGGTACAGC GGGTGCCACG CATTCCCAAG GCAACAAAAT CCGGAAGTGT TACTGACACG 
    GAGAGAGAGG AGAGTGAACA GAGAGCAAAG ACTGAGGAAA CACAGACTGA CACAGATTCC 
    AGCTCTGTGG CTGTAGAGAT GGCGCCCCCT AAAGAAGAAT CTGAGCTGAA GGATTCAACC 
    CCAGCAGCTC CGTCAGTTCT CCAGGACCCC TCTGGTTCTA GTGGTATCGT TGTGCAGGGG 
    CTGACAGAAG ACATGATCAC CATTTTAATC CGTGCCTGTG TCAGCATGAT CAGTGTTCCT 
    GTGGACCCAG ACACTTTGCA CGCCACCTTG CGGCTCTGTC TGCGCCTCAC ACGCACACAC 
    CATTACGCCA TGATGTTTGC AGAGCTCAAG AGCACCCGCA TGATTCTGAA TCTCACCCAG 
    AGCTCAGGCT TTAATGGCTT CACCCCATTG GTCACCCTGC TCTTCCGCCA CATCATAGAG 
    GACCCGGCCA CACTGCGGCA CACTATGGAG AAGGTGGTAC GTTCTGCAGT CACCAGTGGT 
    GCAGGAAGCA CTACTTCAGG TGTGGTGTCA GGTAGTCTGG GCTCCAGGGA AATTAACTAC 
    ATCCTGCGAG TCCTTGGCCC AGCGGCCTGT CGCAATCCAG AGTGCTTTAC GGAAACTGCC 
    AAGAGTTGCA TCCGAATTGC TCTCCCGGCT CCAAGAGGTG CTGGCACTGC CTCAGATGAC 
    GAGTTTGAGA ACTTCAGAAT TAAAGGTCCA AATGCTGTGC AACTCGTGAA GACCACCCCC 
    CTAAAGCTCT CCCCGTTGCC CTCCATACCA GAGACTATAA AAGAGGTCAT CTATGACATG 
    CTGAATGCCC TGGCAGCATA TCACGCACCA GAAGAGGCAG AGAGAGGAGA GGAGCGGGCA 
    AACGCTGTAC CAGGGAATCA AGACCTGTGT CAGATCCTGC AGGATGTCGG TGATGATGTC 
    TACCAGCAGT ACAGACTCAC CAGACAAGGC AGTGATTTTG ACTCGCAATC TGCCTTCCCC 
    ATCAATACTC AGGTGTTTGC TGCAGATGGT GCTGTAGCTG AATCTTCTCA GTCTGGAACT 
    CCACAGGGAG AAGCCTCTAC ACCCGAGGAA ATGCGTGAAG AGAAAAAGGA GCAGGAAGGA 
    GACAAGGGAG CTTCTTCGGA GGAGAGCCGA GCTGCTAAGG CTAAAGCTAG CAAACCTCTG 
    ATGCCCACCT CTACCATCTT GAGGCTGTTG GCCGAGCTGG TTCGCTCTTA TGTTGGCATT 
    GCCACTCTTA TCACCTCTTA CAACTACACA GCTGGCCAGT CGGAGCTCAT TAAGGAAGAT 
    TGCAGTGTGT TGGCATTTGT TTTGGACCAC TTGCTCCCAC ACACCCAGAA CTCTGAAGAT 
    AAGGACACCC CTGCCCTTGC ACGCCTTTTC TTGGCCAGCC TTGCTGCAGC TGGCACGGGC 
    ACCGATGCCC AGGCTGCACT GGTCAACGAG GTCAAAGCTG CTTTGAGTCG AGCATTGGGC 
    ATGGCAGAGG GCACAGAGAA ACACGCCCGT TTGCAAGCAG TGATGTGCAT CATCAGCACC 
    ATTATGGAGT CGTGTCCTTC CACCTCCAGC TTCTACAGCA CAGCCACAGC TAAGACCCAG 
    CACAACGGCA TGAACAATAT CATCCGGCTC TTCTTGAAGA AAGGCCTTGT CAATGACCTG 
    GCCCGTGTGC CTCACAGCCT TGATCTATCC AGTCCCAATA TGGCAAACAC AGTAAATGCT 
    GCTTTGAAGC CTCTGGAAAC ATTGTCGCGA ATTGTGAACC AGCCCAGCAG CCTGTTTGGT 
    GGTAAAGGGG GATCCAGCAA GAACAAGACT GAACATGACA CTGTTGGCAC AGTCAGAGAC 
    AGTAACAGCA ACACTCAGGA TCAAGGAGAA GTGGGTGAGG CAGAGCCGGT TGAGAATCGT 
    GACCGAGGTC AAGCCACAGA CTCTGACCTG ATGGATGGAG AAGCCGAGGG AGACACTGTG 
    GTCATTGCTG GACAGCCTGA GGTGCTGAGC ACTCAGGCAA TGCAGGTGGA GAACGAGTTG 
    GTGGATTTGA TCGATGAACT TTTAGAGAGA GATGCCCAAA CTGTTAATAG TACCATCATC 
    GTAGGTCGTT CTGGAGAGGA TGAGTCTCAA GAGGATGTGC TGATGGATGA AGCTCCTTCC 
    AATATGAGTC AGGCCTCCAC TCTCCAAGCC AACAGAGAAG ACTCCATGAA CATCCTCGAG 
    CCAGAAGATG AGGAGCACAC GCAGGAGGAA GACTCCAGTG GTAGCAACGA GGATGAGGAC 
    AGTCAGGATG AAGAGGAAGA GGAGGAGGAA GAAGAGGAGG AAGATCAGGA TGATGAAGAG 
    GGAGATGAGG ATGATGACGA TGAAGGCTCA GAGATGGAAC TGGATGAAGA TTTTCCAGAT 
    ATCAATGCTG CCCCTCATAT TCGTTTTGAG CGATTCGACC GAGATGATGA CCTCATCATT 
    GAGTTTGATA ATATGTTCTC CACTTCAGCT GACATCCCTC CCTCACCAGG AAATATCCCC 
    AGCTCCCACC CTCTGATGGT GCGTCACGCT GACCATGGCT CGCTGACTCT TGGGGGATCG 
    GGCACCAGTA GCAGATTGGC TCAAGGAATG GGACGTAGTC AACGTTCTCT CCGCCAGCTC 
    ACGGCCAACA CAGGCCACAC GATCCATGTA CATTATCCTG GCAACCGTCA GCCTAACCCT 
    CCACTCATTC TGCAGAGATT ATTGGGCCCC AGCGCTGCAG CTGATATCTT GCAGCTGTCC 
    AGCTCTCTTC CTCTGCAGTC TCGAGGTCGT GCCCGTTTAA TGGTGGGTAA TGAAGATGTG 
    CACATTATTG CCCGTTCAGA CGATGAGCTG CTGGATGACT TCTTCCATGA GCAGAGCAGC 
    ACCGGAGGAC AAGCTGGCAC CCTTTCAAGT ATCCCGACTG CCTTAACCAG ATGGACAGAT 
    GAGTGTAAAG TGCTGGATGC TGAGAGCATG CATGACTGTG TGGCAGTGGT GAAGGTGCCC 
    ATACTGCAGC ACCTGGAGAG TTTGCGTGAT GAGGAGCTGG AGGAACGCAG GGAGAAAAGG 
    AGGAGGCAGT TGGCAGAGGA AGAGGAGACT GCAAAGCAGA ATGAGAGTTC TGCAGCAGGG 
    GAGGAGACCA GGGAACAGAG TCTGCAGGGC TCTGGGATTG GAACAGTCAA TGGAGCTGCA 
    GAGAATGCTG CAGAAGGAGA ACAGGCCCAG AGTGGGGTGT CCTCCTGTCT GGACCCTCCT 
    CGCACCTCTG AGGGTTTCCT CACAGCGCCC CCATCAGGAG AAGTCACTCC AACCACCCCT 
    GCCCCCCATG AACAAGCCCT CGTGTCTCTG GAAACAGCCA TCAGTCAGCA GGTGCACCAG 
    CCAATCACAG AGCTGCTGCT GGCCGACTCC AATCCTGACT CTCACGGAGC CTTGGCAGGG 
    GCAGGGCTTC CCCAGCTCTC AGCACCTGAT AGGCCAAATT GTGAGGCAGA AGCATCTCAG 
    ATGGAGATGT CACCTGCACC CACTATTGGT GAGAGAGGAG GAGGAGCATT GGATGCAGTC 
    AGGGAAGCTT CACTCAGTCC AGATATAGCT GAAAGTTCAG AGCCTGTGCC AGTGGGTGTG 
    TCTCAACTCG AGGGTTCGCC CATGGATACG AGCAGTCCCG CTTCAGGAAC GCAGGAAGAG 
    CCGGCTGCCA GCTCAGTTCC ATCCAATCAC ATGTCTCAGG AGCTATCAGG AAGTGGTGAC 
    AGCGGCCTCA CAGACAGGCA GACAGATGCA GAAACTGGAT CAACTTCTGT TTCATCTCCT 
    GGCGAGACCA TGCCTCGGAG TGACAGTGCC GATAGCCAGT CGCAGGCCAT CCAGGAGGAG 
    CCCCTCCCTT CCACCAGCAA TGAAGAGGAG GACCCTCTGG CAGGCATCAG TCTACCTGAA 
    GGCGTGGATC CGTCTTTCTT GGCAGCACTG CCTGAAGACA TTCGTCGGGA GGTGCTGCAG 
    AACCAACTGG GAATCAGACC TCCTTCTCGA CCTCCTGTCG CCTCCACTCT GACAACCACT 
    GCTAGTGCTG TGCTAGGAGG AGGGCCAGGG GTGACCGAAG TCAGCCCTGA ATTCTTAGCG 
    GCTCTCCCAC CTGCAATTCA GGAAGAGGTC CTGGCCCAGC AGCGGGCAGA GCAGCAGCGT 
    CGGGAGTTAT CCCAGCAACC CACACAGGGT GATCAACCTC TGGACCCAGT CACATTCATC 
    CAGACCCTGC CCTCTGAGCT GCGGCGCTCC GTTCTGGAAG ACATGGAGGA CAGTGTACTA 
    GCGGTCATGC CTCCAGATAT TGCAGCAGAG GCTGCAGCAC TGCGAAGGGA ACAAGAGGCC 
    AGACAGAGAC AACTGATGCA CGAGCGCTTG TTCGGTCACA GCTCCTCTTC AGCTCTGTCT 
    GCCATCTTAC GCTCTCCCGC CTTCACCAGC CGCCTCGGGG GCAACCGTGG GGTACAGTAC 
    ACCCGGCTGG CTGTGCAAAG AGGAGGGACG TTTCAAATGG GAGGGAGTGC CAATCATAGC 
    AGACCCTCTA GTTCAAACGT TGACTCTCTC CTGCGCCTGC GGGGGCGTCT CTTGTTGGAC 
    CACGAGGCCT TGTCCTGCCT TCTTGTGCTG CTTTTTGTGG ACGAGCCCAA ACTTAACACC 
    AGCCGCCTCC ACCGAGTGCT GCGTAACTTG TGCTATCACT CTCAGACGCG TGGCTGGGTC 
    ATCCGCAGTC TCCTGTCCAT CCTGCAGCGC AGCAGTGAGA GCGAGCTGTG CCTGGAGACT 
    GCACGCTTGG AGGAGTCCCG TGGCAAACGC TCACTAGGCC AGGGCTCATC TAGTGGAGGA 
    AAGGGTTGTT CTTCATCCTC CCAGTCGGCT ATAGCCTCTT CGTCATCGTC CTCCTCGTCC 
    TCTTCTTCTC TGGAGCTGCT GAACAGGGTG GAGTCACGGA GTTCCAGTCA GCTCTCCTGG 
    CTGTCTGTAT CAATGGACGC AGCTCTTGGA TGCAGAACCA ACATCTTTCA GATCCAGCGA 
    GTATCAGGCA GGAAACACGC AGAGAGGCAT TCAGCAGGAG GGAGTGTGAG CGCAGGAGGA 
    CTCACAGGTG GAGTGTCAGG AACAGGAATG ACGTGTACAG GGGGAGGTGG GTCAACTGTT 
    CACATCCATC CACAGGCTGC GCCGGTGGTC TGTCGACATG TACTGGACAC CCTCATCCAA 
    TTGGCCAAGG TATTTCCAAG TCACTTCACT CAACAACGCT GTAAAGATCC CCTATCCTCC 
    TCCTCCTCTG AGTTGGACAC TCGTCTTTGT GCCAGCTCAA CACCGACTAT GAGTGCCAGT 
    TGTCGGTCAG GTGGCACCAG TCAGAGTAAC TCCTCTGCTA CCCCAAGTAG TCTCACTTCT 
    ATCCACAATT CCCTGAGCGC CTGCTCTGGT ACGCCACAGT CCCTAGGCAT CTCCACCGAC 
    TTTTGGGATC TCCTGGTCAA GCTGGACAAC ATGAATGTGA GCCGAAAGGG GAAGGCCTCC 
    ATGAAGACTG TCCCTCTAGG GGGTGTCGGC GAGACAGAGG GTGCGCTGTT CAGCCTTGAG 
    GCGTCTCCGC TGGGCCAGCT GATGAATATG CTGTCCCACC CTGTGATCCG CCGTAGTTCC 
    CTCCTCACGG AGAAACTGCT ACGTCTGCTG TCTCTGATCT CCATCGCTCT GCCTGACAAC 
    AAGGCCACGG AGGTTCCAGC CAGCCATCCA GTGCCTCAGA CCCCAACTGC CACGCCATCT 
    GCACCTGCTC CTCAAACCAC AGCAGGGTCA GGGACCATCT CAGCAGTGGC AGCTACAGGC 
    ACTACGCAGG GTTCAGTCGG TTCAGTGGGT ACAACGCCAT CTCAACCAGC CGCTACAATA 
    TCTGTGGTTG CAGGCAGCAC CACCGCCTCT AGCATTATTG CTGGAAAACT GAAGCCAGCA 
    GTAAGCATCA CGGACATTGA ATCTAAACTG GCCTCTACTG GACTTACGGA GAAGCAGCTG 
    CAGCTCTCTG TGGAGGTTCT GACATCTCAC TCGTGTTCAG AGGAAGGTCT GGAGGACGCA 
    GCCAACATCT TGCTGCAGCT GTCCAGAGGG GACGCACCGA CCCGAGACAC TGTGCTTAGA 
    CTTCTGCTCA GCGGAGCACG TCACCTTGGA TACACTCTCT GCAAACAAAT CGGCACTTTA 
    TTAGCTGAGT TGAGGGAGTA TAATCTAGAG CAGCAGAGAA GAGCACGGGC TGATGCTCAG 
    TCTCCTGAAG CTTCTCATGA TGACCCATCC ATCACCACAC GCCTCAAGGG CAAGATGACC 
    AGCAGGTTTG ACGGGTCAGA GAGTGTGGTG ATTGTTGCTG CTCAGAAGCG CACGTTAGGT 
    GGCCGGGAGC TTCAGTTGCC CTGCATGAGC TCGCTCACTT CCAAGACCTC AACGCAGAAG 
    TTTTTCTTGC GGGTACTGCA GGTCATCATC CAGCTTAGAG AAGACACGCG GCGCGCTAAC 
    AAGAAAGCCA AGCAGACAGG AGGAAGACTA AGTTCAACCA GTCTGGGCTC GGCCAGTAGT 
    ATTCAGGCTG CGGTCCGTCA GCTGGAGGCT GAAGCGGACG CCATCATTCA GATGGTGAGA 
    GAGGGACAGA GGGCACGCCG TCTACAGCAG GCCCCCCCAC CCTCTTCATC TGCATCCTCT 
    TCCGCTGCTG CTGCTGCTGC ATCCGCTGCT GTGGCTGTGG CTGGATCGTC TGCCCCCAGT 
    ACCTCTGCAA CCAACAACCC CCCCGCTGGC ACGGCCAGCG CAGCCACGTC GGAGGTTCAC 
    ACTGTTGCCG AGCCACCAGC AGCTCAGAGA GATGATTCGC CCATGGATGT GGATCAGCCC 
    TCTCCTTTGG AACAGGACCA GCCAACATTA GGTGAGGAGA GCAGCAGTCA GCAGGAGCAG 
    GAGGAGCGTT TGCCTGACCT GCCGCTGTTA AGTGAGCAGC TGCTTTTGGA TGAGCTGTGG 
    GACATGCTTG GAGAATGTCT GAAAGAGCTT GAAGAGTCTC ATGACCAACA TGCAGTTCTA 
    GTGCTGCAGC CTGCAGTGGA GGCCTTTTTC TTGGTGCATG CAACAGAGCG GGAAAGCAAA 
    CCACCAGTTC GGGACACTCG GGAGAGCCAG CTGTCACACA TCAAGGATGA GCCTCCACCC 
    CTGTCACCCG CCCCTCTCAC ACCCGCTACC CCCTCCTCTC TGGACCCCTT TTTTTCCAGA 
    GAGCCTTCCT CTATGCACAT CTCTTCCAAC CTGCCTCCAG ATACACAGAA GTTCCTCCGT 
    TTCGCAGAGA CCCACCGCAC AGTGCTGAAT CAGATCCTGC GTCAATCCAC CACTCACCTG 
    GCAGACGGGC CTTTCGCTGT TTTGGTTGAC TACATTCGCA TCTTGGACTT TGACGTCAAG 
    CGCAAGTATT TCCGTCAGGA GCTGGAGCGT CTGGATGAAG GCTTGAGGAA AGAGGATATG 
    GCCGTCCATG TGAGGAGAGA TCACGTCTTT GAAGATTCTT ACAGAGAGCT GCACCGCAAG 
    AGCCCAGAAG ACATGAAGAA CAGATTGTAT ATAGTATTTG AAGGAGAGGA GGGTCAGGAT 
    GCAGGCGGGC TGTTAAGAGA GTGGTACATG ATCATCTCCA GAGAAATGTT CAACCCCATG 
    TATGCCCTGT TTCGCACCTC CCCCGGCGAT CGGGTCACCT ATACCATCAA CCCCTCCTCC 
    CACTGCAACC CCAACCATCT CAGCTACTTC AAGTTTGTGG GACGTGTGGT GGCCAAAGCA 
    GTCTACGACA ACAGGCTATT AGAGTGTTAC TTTACACGCA GCTTCTACAA GCACATCCTG 
    GGCAAGAGCG TTAGGTATAC AGACATGGAA AGCGAAGACT ATCCCTTTTT CCAAGGTCTG 
    GTTTATTTGT TGGAGAACGA TGTGTCGACT CTGGGCTACG AGCTGACATT CAGCACAGAG 
    GTCCAAGAGT TTGGCGTGTG TGAAGTGAGA GATCTGAAGG CCAACGGTGC CAACATTCTT 
    GTCACAGAAG AGAATAAGAA GGAATATGTG CATCTGGTCT GCCAGATGAA GATGACAGGT 
    GCAATCCGTA AACAGCTGGC TGCCTTCCTG GAGGGCTTTT ATGAGATCAT CCCCAAGAGA 
    CTGATCTCCA TCTTCACTGA GCAAGAGCTG GAGCTGCTCA TCTCCGGCCT GCCCACCATT 
    GACATCGACG ACCTCAAGGC CAATACTGAA TACCATAAAT ATCAGTCCAG TTCTATTCAG 
    ATCCAGTGGT TCTGGCGTGC TTTACGATCC TTCGACCAGG CTGACAGGGC CAAATTTCTG 
    CAGTTTGTTA CGGGCACTTC CAAGGTGCCA CTGCAGGGCT TTGCTGCACT GGAGGGAATG 
    AACGGCATTC AGAAGTTCCA GATCCACCGA GATGACCGCT CCACTGACCG CCTGCCCTCT 
    GCGCACACTT GCTTTAACCA GTTGGACCTC CCAGCGTACG AGAGCTACGA GAAGTTAAGA 
    CACATGCTGC TGCTGGCCAT TCAGGAATGT TCTGAAGGCT TCGGACTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_021473710.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329385.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID ODa27859
    Clone ID Related Accession (Same CDS sequence) XM_005162282.3 , XM_021473710.1 , XM_005162282.4
    Accession Version XM_021473710.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    541
    601
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    721
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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
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    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
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    3361
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    3481
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4561
    4621
    4681
    4741
    4801
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    4921
    4981
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    5281
    5341
    5401
    5461
    5521
    5581
    5641
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    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
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    6601
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    7021
    7081
    7141
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    7381
    7441
    7501
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    7741
    7801
    7861
    7921
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    8401
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    8641
    8701
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    8821
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    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAAGCAGCT 
    CGCAGGCGTG AGGAGGAGGA GAGGAGAGCG AGGGAGCGGG TAGAGGAGGA AGAAGCTCGT 
    TGTCTGGAGC GCTTTCTGGA AGCAGAGCCT CTTGACACCA CTGAGCTTCA TGCCTTCACA 
    GACTCCATGC TGCCTGGCTG CTTCCACCTG CTCGATGAGC TGCCGGACAC AGTCTACCGC 
    CTCTGTGATC TGCTCATGAC TGCTATTAAA AGAAACGGCC CTGAATACAG AGACCTCATC 
    CTTAGACAAG TGGTCAATCA GGTGTGGGAG GCTGCTGACG TGTTGATAAA GGCTGCAGTG 
    CCGCTAACCA CCAGCGACAC CAAGACAGTC TCTGAGTGGA CCAGACAGAT GGCGACCCTC 
    CCTCAGGCCT CCAACCTGGC CACCCGCATC CTGCTGCTCA CGCTGCTCTT TGAGGAGCTG 
    AAACTCTCCT GCGCTCGGGT GGTGGAGAGC AGTGGTGTCT TGACTGTGCT GATTAAGCTG 
    TTGGAGGTGG TGCAGCCCTG TCTGCAAGCT GCCAAAGAGC AGAAGGACAT CCAAACGCCC 
    AAATGGATCA CCCCTGTGCT CCTACTTATT GACTTTTATG AGAAAATGGC TGTTTCATCC 
    AAACGAAGAG CTCAGATGAA CAAGTACCTC CAGCCCAATG GGAATAACTG GCGCTGGTTT 
    GATGACCGTT CAGGCCGCTG GTGCAGCTAC AGTGCAAGTA ACAACAGCAC TATTGACTCT 
    GCATGGAGGG CAGGAGAGAC CAGTGTCCGA TTTACAGCTG GCCGCAGAAG ATACACAGTT 
    CAGTTCAACA CCATGGTGCA GGTAAATGAG GAGACTGGCA ACAGACGACC AGTTATGTTG 
    ACGGTACAGC GGGTGCCACG CATTCCCAAG GCAACAAAAT CCGGAAGTGT TACTGACACG 
    GAGAGAGAGG AGAGTGAACA GAGAGCAAAG ACTGAGGAAA CACAGACTGA CACAGATTCC 
    AGCTCTGTGG CTGTAGAGAT GGCGCCCCCT AAAGAAGAAT CTGAGCTGAA GGATTCAACC 
    CCAGCAGCTC CGTCAGTTCT CCAGGACCCC TCTGGTTCTA GTGGTATCGT TGTGCAGGGG 
    CTGACAGAAG ACATGATCAC CATTTTAATC CGTGCCTGTG TCAGCATGAT CAGTGTTCCT 
    GTGGACCCAG ACACTTTGCA CGCCACCTTG CGGCTCTGTC TGCGCCTCAC ACGCACACAC 
    CATTACGCCA TGATGTTTGC AGAGCTCAAG AGCACCCGCA TGATTCTGAA TCTCACCCAG 
    AGCTCAGGCT TTAATGGCTT CACCCCATTG GTCACCCTGC TCTTCCGCCA CATCATAGAG 
    GACCCGGCCA CACTGCGGCA CACTATGGAG AAGGTGGTAC GTTCTGCAGT CACCAGTGGT 
    GCAGGAAGCA CTACTTCAGG TGTGGTGTCA GGTAGTCTGG GCTCCAGGGA AATTAACTAC 
    ATCCTGCGAG TCCTTGGCCC AGCGGCCTGT CGCAATCCAG AGTGCTTTAC GGAAACTGCC 
    AAGAGTTGCA TCCGAATTGC TCTCCCGGCT CCAAGAGGTG CTGGCACTGC CTCAGATGAC 
    GAGTTTGAGA ACTTCAGAAT TAAAGGTCCA AATGCTGTGC AACTCGTGAA GACCACCCCC 
    CTAAAGCTCT CCCCGTTGCC CTCCATACCA GAGACTATAA AAGAGGTCAT CTATGACATG 
    CTGAATGCCC TGGCAGCATA TCACGCACCA GAAGAGGCAG AGAGAGGAGA GGAGCGGGCA 
    AACGCTGTAC CAGGGAATCA AGACCTGTGT CAGATCCTGC AGGATGTCGG TGATGATGTC 
    TACCAGCAGT ACAGACTCAC CAGACAAGGC AGTGATTTTG ACTCGCAATC TGCCTTCCCC 
    ATCAATACTC AGGTGTTTGC TGCAGATGGT GCTGTAGCTG AATCTTCTCA GTCTGGAACT 
    CCACAGGGAG AAGCCTCTAC ACCCGAGGAA ATGCGTGAAG AGAAAAAGGA GCAGGAAGGA 
    GACAAGGGAG CTTCTTCGGA GGAGAGCCGA GCTGCTAAGG CTAAAGCTAG CAAACCTCTG 
    ATGCCCACCT CTACCATCTT GAGGCTGTTG GCCGAGCTGG TTCGCTCTTA TGTTGGCATT 
    GCCACTCTTA TCACCTCTTA CAACTACACA GCTGGCCAGT CGGAGCTCAT TAAGGAAGAT 
    TGCAGTGTGT TGGCATTTGT TTTGGACCAC TTGCTCCCAC ACACCCAGAA CTCTGAAGAT 
    AAGGACACCC CTGCCCTTGC ACGCCTTTTC TTGGCCAGCC TTGCTGCAGC TGGCACGGGC 
    ACCGATGCCC AGGCTGCACT GGTCAACGAG GTCAAAGCTG CTTTGAGTCG AGCATTGGGC 
    ATGGCAGAGG GCACAGAGAA ACACGCCCGT TTGCAAGCAG TGATGTGCAT CATCAGCACC 
    ATTATGGAGT CGTGTCCTTC CACCTCCAGC TTCTACAGCA CAGCCACAGC TAAGACCCAG 
    CACAACGGCA TGAACAATAT CATCCGGCTC TTCTTGAAGA AAGGCCTTGT CAATGACCTG 
    GCCCGTGTGC CTCACAGCCT TGATCTATCC AGTCCCAATA TGGCAAACAC AGTAAATGCT 
    GCTTTGAAGC CTCTGGAAAC ATTGTCGCGA ATTGTGAACC AGCCCAGCAG CCTGTTTGGT 
    GGTAAAGGGG GATCCAGCAA GAACAAGACT GAACATGACA CTGTTGGCAC AGTCAGAGAC 
    AGTAACAGCA ACACTCAGGA TCAAGGAGAA GTGGGTGAGG CAGAGCCGGT TGAGAATCGT 
    GACCGAGGTC AAGCCACAGA CTCTGACCTG ATGGATGGAG AAGCCGAGGG AGACACTGTG 
    GTCATTGCTG GACAGCCTGA GGTGCTGAGC ACTCAGGCAA TGCAGGTGGA GAACGAGTTG 
    GTGGATTTGA TCGATGAACT TTTAGAGAGA GATGCCCAAA CTGTTAATAG TACCATCATC 
    GTAGGTCGTT CTGGAGAGGA TGAGTCTCAA GAGGATGTGC TGATGGATGA AGCTCCTTCC 
    AATATGAGTC AGGCCTCCAC TCTCCAAGCC AACAGAGAAG ACTCCATGAA CATCCTCGAG 
    CCAGAAGATG AGGAGCACAC GCAGGAGGAA GACTCCAGTG GTAGCAACGA GGATGAGGAC 
    AGTCAGGATG AAGAGGAAGA GGAGGAGGAA GAAGAGGAGG AAGATCAGGA TGATGAAGAG 
    GGAGATGAGG ATGATGACGA TGAAGGCTCA GAGATGGAAC TGGATGAAGA TTTTCCAGAT 
    ATCAATGCTG CCCCTCATAT TCGTTTTGAG CGATTCGACC GAGATGATGA CCTCATCATT 
    GAGTTTGATA ATATGTTCTC CACTTCAGCT GACATCCCTC CCTCACCAGG AAATATCCCC 
    AGCTCCCACC CTCTGATGGT GCGTCACGCT GACCATGGCT CGCTGACTCT TGGGGGATCG 
    GGCACCAGTA GCAGATTGGC TCAAGGAATG GGACGTAGTC AACGTTCTCT CCGCCAGCTC 
    ACGGCCAACA CAGGCCACAC GATCCATGTA CATTATCCTG GCAACCGTCA GCCTAACCCT 
    CCACTCATTC TGCAGAGATT ATTGGGCCCC AGCGCTGCAG CTGATATCTT GCAGCTGTCC 
    AGCTCTCTTC CTCTGCAGTC TCGAGGTCGT GCCCGTTTAA TGGTGGGTAA TGAAGATGTG 
    CACATTATTG CCCGTTCAGA CGATGAGCTG CTGGATGACT TCTTCCATGA GCAGAGCAGC 
    ACCGGAGGAC AAGCTGGCAC CCTTTCAAGT ATCCCGACTG CCTTAACCAG ATGGACAGAT 
    GAGTGTAAAG TGCTGGATGC TGAGAGCATG CATGACTGTG TGGCAGTGGT GAAGGTGCCC 
    ATACTGCAGC ACCTGGAGAG TTTGCGTGAT GAGGAGCTGG AGGAACGCAG GGAGAAAAGG 
    AGGAGGCAGT TGGCAGAGGA AGAGGAGACT GCAAAGCAGA ATGAGAGTTC TGCAGCAGGG 
    GAGGAGACCA GGGAACAGAG TCTGCAGGGC TCTGGGATTG GAACAGTCAA TGGAGCTGCA 
    GAGAATGCTG CAGAAGGAGA ACAGGCCCAG AGTGGGGTGT CCTCCTGTCT GGACCCTCCT 
    CGCACCTCTG AGGGTTTCCT CACAGCGCCC CCATCAGGAG AAGTCACTCC AACCACCCCT 
    GCCCCCCATG AACAAGCCCT CGTGTCTCTG GAAACAGCCA TCAGTCAGCA GGTGCACCAG 
    CCAATCACAG AGCTGCTGCT GGCCGACTCC AATCCTGACT CTCACGGAGC CTTGGCAGGG 
    GCAGGGCTTC CCCAGCTCTC AGCACCTGAT AGGCCAAATT GTGAGGCAGA AGCATCTCAG 
    ATGGAGATGT CACCTGCACC CACTATTGGT GAGAGAGGAG GAGGAGCATT GGATGCAGTC 
    AGGGAAGCTT CACTCAGTCC AGATATAGCT GAAAGTTCAG AGCCTGTGCC AGTGGGTGTG 
    TCTCAACTCG AGGGTTCGCC CATGGATACG AGCAGTCCCG CTTCAGGAAC GCAGGAAGAG 
    CCGGCTGCCA GCTCAGTTCC ATCCAATCAC ATGTCTCAGG AGCTATCAGG AAGTGGTGAC 
    AGCGGCCTCA CAGACAGGCA GACAGATGCA GAAACTGGAT CAACTTCTGT TTCATCTCCT 
    GGCGAGACCA TGCCTCGGAG TGACAGTGCC GATAGCCAGT CGCAGGCCAT CCAGGAGGAG 
    CCCCTCCCTT CCACCAGCAA TGAAGAGGAG GACCCTCTGG CAGGCATCAG TCTACCTGAA 
    GGCGTGGATC CGTCTTTCTT GGCAGCACTG CCTGAAGACA TTCGTCGGGA GGTGCTGCAG 
    AACCAACTGG GAATCAGACC TCCTTCTCGA CCTCCTGTCG CCTCCACTCT GACAACCACT 
    GCTAGTGCTG TGCTAGGAGG AGGGCCAGGG GTGACCGAAG TCAGCCCTGA ATTCTTAGCG 
    GCTCTCCCAC CTGCAATTCA GGAAGAGGTC CTGGCCCAGC AGCGGGCAGA GCAGCAGCGT 
    CGGGAGTTAT CCCAGCAACC CACACAGGGT GATCAACCTC TGGACCCAGT CACATTCATC 
    CAGACCCTGC CCTCTGAGCT GCGGCGCTCC GTTCTGGAAG ACATGGAGGA CAGTGTACTA 
    GCGGTCATGC CTCCAGATAT TGCAGCAGAG GCTGCAGCAC TGCGAAGGGA ACAAGAGGCC 
    AGACAGAGAC AACTGATGCA CGAGCGCTTG TTCGGTCACA GCTCCTCTTC AGCTCTGTCT 
    GCCATCTTAC GCTCTCCCGC CTTCACCAGC CGCCTCGGGG GCAACCGTGG GGTACAGTAC 
    ACCCGGCTGG CTGTGCAAAG AGGAGGGACG TTTCAAATGG GAGGGAGTGC CAATCATAGC 
    AGACCCTCTA GTTCAAACGT TGACTCTCTC CTGCGCCTGC GGGGGCGTCT CTTGTTGGAC 
    CACGAGGCCT TGTCCTGCCT TCTTGTGCTG CTTTTTGTGG ACGAGCCCAA ACTTAACACC 
    AGCCGCCTCC ACCGAGTGCT GCGTAACTTG TGCTATCACT CTCAGACGCG TGGCTGGGTC 
    ATCCGCAGTC TCCTGTCCAT CCTGCAGCGC AGCAGTGAGA GCGAGCTGTG CCTGGAGACT 
    GCACGCTTGG AGGAGTCCCG TGGCAAACGC TCACTAGGCC AGGGCTCATC TAGTGGAGGA 
    AAGGGTTGTT CTTCATCCTC CCAGTCGGCT ATAGCCTCTT CGTCATCGTC CTCCTCGTCC 
    TCTTCTTCTC TGGAGCTGCT GAACAGGGTG GAGTCACGGA GTTCCAGTCA GCTCTCCTGG 
    CTGTCTGTAT CAATGGACGC AGCTCTTGGA TGCAGAACCA ACATCTTTCA GATCCAGCGA 
    GTATCAGGCA GGAAACACGC AGAGAGGCAT TCAGCAGGAG GGAGTGTGAG CGCAGGAGGA 
    CTCACAGGTG GAGTGTCAGG AACAGGAATG ACGTGTACAG GGGGAGGTGG GTCAACTGTT 
    CACATCCATC CACAGGCTGC GCCGGTGGTC TGTCGACATG TACTGGACAC CCTCATCCAA 
    TTGGCCAAGG TATTTCCAAG TCACTTCACT CAACAACGCT GTAAAGATCC CCTATCCTCC 
    TCCTCCTCTG AGTTGGACAC TCGTCTTTGT GCCAGCTCAA CACCGACTAT GAGTGCCAGT 
    TGTCGGTCAG GTGGCACCAG TCAGAGTAAC TCCTCTGCTA CCCCAAGTAG TCTCACTTCT 
    ATCCACAATT CCCTGAGCGC CTGCTCTGGT ACGCCACAGT CCCTAGGCAT CTCCACCGAC 
    TTTTGGGATC TCCTGGTCAA GCTGGACAAC ATGAATGTGA GCCGAAAGGG GAAGGCCTCC 
    ATGAAGACTG TCCCTCTAGG GGGTGTCGGC GAGACAGAGG GTGCGCTGTT CAGCCTTGAG 
    GCGTCTCCGC TGGGCCAGCT GATGAATATG CTGTCCCACC CTGTGATCCG CCGTAGTTCC 
    CTCCTCACGG AGAAACTGCT ACGTCTGCTG TCTCTGATCT CCATCGCTCT GCCTGACAAC 
    AAGGCCACGG AGGTTCCAGC CAGCCATCCA GTGCCTCAGA CCCCAACTGC CACGCCATCT 
    GCACCTGCTC CTCAAACCAC AGCAGGGTCA GGGACCATCT CAGCAGTGGC AGCTACAGGC 
    ACTACGCAGG GTTCAGTCGG TTCAGTGGGT ACAACGCCAT CTCAACCAGC CGCTACAATA 
    TCTGTGGTTG CAGGCAGCAC CACCGCCTCT AGCATTATTG CTGGAAAACT GAAGCCAGCA 
    GTAAGCATCA CGGACATTGA ATCTAAACTG GCCTCTACTG GACTTACGGA GAAGCAGCTG 
    CAGCTCTCTG TGGAGGTTCT GACATCTCAC TCGTGTTCAG AGGAAGGTCT GGAGGACGCA 
    GCCAACATCT TGCTGCAGCT GTCCAGAGGG GACGCACCGA CCCGAGACAC TGTGCTTAGA 
    CTTCTGCTCA GCGGAGCACG TCACCTTGGA TACACTCTCT GCAAACAAAT CGGCACTTTA 
    TTAGCTGAGT TGAGGGAGTA TAATCTAGAG CAGCAGAGAA GAGCACGGGC TGATGCTCAG 
    TCTCCTGAAG CTTCTCATGA TGACCCATCC ATCACCACAC GCCTCAAGGG CAAGATGACC 
    AGCAGGTTTG ACGGGTCAGA GAGTGTGGTG ATTGTTGCTG CTCAGAAGCG CACGTTAGGT 
    GGCCGGGAGC TTCAGTTGCC CTGCATGAGC TCGCTCACTT CCAAGACCTC AACGCAGAAG 
    TTTTTCTTGC GGGTACTGCA GGTCATCATC CAGCTTAGAG AAGACACGCG GCGCGCTAAC 
    AAGAAAGCCA AGCAGACAGG AGGAAGACTA AGTTCAACCA GTCTGGGCTC GGCCAGTAGT 
    ATTCAGGCTG CGGTCCGTCA GCTGGAGGCT GAAGCGGACG CCATCATTCA GATGGTGAGA 
    GAGGGACAGA GGGCACGCCG TCTACAGCAG GCCCCCCCAC CCTCTTCATC TGCATCCTCT 
    TCCGCTGCTG CTGCTGCTGC ATCCGCTGCT GTGGCTGTGG CTGGATCGTC TGCCCCCAGT 
    ACCTCTGCAA CCAACAACCC CCCCGCTGGC ACGGCCAGCG CAGCCACGTC GGAGGTTCAC 
    ACTGTTGCCG AGCCACCAGC AGCTCAGAGA GATGATTCGC CCATGGATGT GGATCAGCCC 
    TCTCCTTTGG AACAGGACCA GCCAACATTA GGTGAGGAGA GCAGCAGTCA GCAGGAGCAG 
    GAGGAGCGTT TGCCTGACCT GCCGCTGTTA AGTGAGCAGC TGCTTTTGGA TGAGCTGTGG 
    GACATGCTTG GAGAATGTCT GAAAGAGCTT GAAGAGTCTC ATGACCAACA TGCAGTTCTA 
    GTGCTGCAGC CTGCAGTGGA GGCCTTTTTC TTGGTGCATG CAACAGAGCG GGAAAGCAAA 
    CCACCAGTTC GGGACACTCG GGAGAGCCAG CTGTCACACA TCAAGGATGA GCCTCCACCC 
    CTGTCACCCG CCCCTCTCAC ACCCGCTACC CCCTCCTCTC TGGACCCCTT TTTTTCCAGA 
    GAGCCTTCCT CTATGCACAT CTCTTCCAAC CTGCCTCCAG ATACACAGAA GTTCCTCCGT 
    TTCGCAGAGA CCCACCGCAC AGTGCTGAAT CAGATCCTGC GTCAATCCAC CACTCACCTG 
    GCAGACGGGC CTTTCGCTGT TTTGGTTGAC TACATTCGCA TCTTGGACTT TGACGTCAAG 
    CGCAAGTATT TCCGTCAGGA GCTGGAGCGT CTGGATGAAG GCTTGAGGAA AGAGGATATG 
    GCCGTCCATG TGAGGAGAGA TCACGTCTTT GAAGATTCTT ACAGAGAGCT GCACCGCAAG 
    AGCCCAGAAG ACATGAAGAA CAGATTGTAT ATAGTATTTG AAGGAGAGGA GGGTCAGGAT 
    GCAGGCGGGC TGTTAAGAGA GTGGTACATG ATCATCTCCA GAGAAATGTT CAACCCCATG 
    TATGCCCTGT TTCGCACCTC CCCCGGCGAT CGGGTCACCT ATACCATCAA CCCCTCCTCC 
    CACTGCAACC CCAACCATCT CAGCTACTTC AAGTTTGTGG GACGTGTGGT GGCCAAAGCA 
    GTCTACGACA ACAGGCTATT AGAGTGTTAC TTTACACGCA GCTTCTACAA GCACATCCTG 
    GGCAAGAGCG TTAGGTATAC AGACATGGAA AGCGAAGACT ATCCCTTTTT CCAAGGTCTG 
    GTTTATTTGT TGGAGAACGA TGTGTCGACT CTGGGCTACG AGCTGACATT CAGCACAGAG 
    GTCCAAGAGT TTGGCGTGTG TGAAGTGAGA GATCTGAAGG CCAACGGTGC CAACATTCTT 
    GTCACAGAAG AGAATAAGAA GGAATATGTG CATCTGGTCT GCCAGATGAA GATGACAGGT 
    GCAATCCGTA AACAGCTGGC TGCCTTCCTG GAGGGCTTTT ATGAGATCAT CCCCAAGAGA 
    CTGATCTCCA TCTTCACTGA GCAAGAGCTG GAGCTGCTCA TCTCCGGCCT GCCCACCATT 
    GACATCGACG ACCTCAAGGC CAATACTGAA TACCATAAAT ATCAGTCCAG TTCTATTCAG 
    ATCCAGTGGT TCTGGCGTGC TTTACGATCC TTCGACCAGG CTGACAGGGC CAAATTTCTG 
    CAGTTTGTTA CGGGCACTTC CAAGGTGCCA CTGCAGGGCT TTGCTGCACT GGAGGGAATG 
    AACGGCATTC AGAAGTTCCA GATCCACCGA GATGACCGCT CCACTGACCG CCTGCCCTCT 
    GCGCACACTT GCTTTAACCA GTTGGACCTC CCAGCGTACG AGAGCTACGA GAAGTTAAGA 
    CACATGCTGC TGCTGGCCAT TCAGGAATGT TCTGAAGGCT TCGGACTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329385.1
    CDS142..13755
    Translation

    Target ORF information:

    RefSeq Version XM_021473710.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021473710.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAAGCAGCT 
    CGCAGGCGTG AGGAGGAGGA GAGGAGAGCG AGGGAGCGGG TAGAGGAGGA AGAAGCTCGT 
    TGTCTGGAGC GCTTTCTGGA AGCAGAGCCT CTTGACACCA CTGAGCTTCA TGCCTTCACA 
    GACTCCATGC TGCCTGGCTG CTTCCACCTG CTCGATGAGC TGCCGGACAC AGTCTACCGC 
    CTCTGTGATC TGCTCATGAC TGCTATTAAA AGAAACGGCC CTGAATACAG AGACCTCATC 
    CTTAGACAAG TGGTCAATCA GGTGTGGGAG GCTGCTGACG TGTTGATAAA GGCTGCAGTG 
    CCGCTAACCA CCAGCGACAC CAAGACAGTC TCTGAGTGGA CCAGACAGAT GGCGACCCTC 
    CCTCAGGCCT CCAACCTGGC CACCCGCATC CTGCTGCTCA CGCTGCTCTT TGAGGAGCTG 
    AAACTCTCCT GCGCTCGGGT GGTGGAGAGC AGTGGTGTCT TGACTGTGCT GATTAAGCTG 
    TTGGAGGTGG TGCAGCCCTG TCTGCAAGCT GCCAAAGAGC AGAAGGACAT CCAAACGCCC 
    AAATGGATCA CCCCTGTGCT CCTACTTATT GACTTTTATG AGAAAATGGC TGTTTCATCC 
    AAACGAAGAG CTCAGATGAA CAAGTACCTC CAGCCCAATG GGAATAACTG GCGCTGGTTT 
    GATGACCGTT CAGGCCGCTG GTGCAGCTAC AGTGCAAGTA ACAACAGCAC TATTGACTCT 
    GCATGGAGGG CAGGAGAGAC CAGTGTCCGA TTTACAGCTG GCCGCAGAAG ATACACAGTT 
    CAGTTCAACA CCATGGTGCA GGTAAATGAG GAGACTGGCA ACAGACGACC AGTTATGTTG 
    ACGGTACAGC GGGTGCCACG CATTCCCAAG GCAACAAAAT CCGGAAGTGT TACTGACACG 
    GAGAGAGAGG AGAGTGAACA GAGAGCAAAG ACTGAGGAAA CACAGACTGA CACAGATTCC 
    AGCTCTGTGG CTGTAGAGAT GGCGCCCCCT AAAGAAGAAT CTGAGCTGAA GGATTCAACC 
    CCAGCAGCTC CGTCAGTTCT CCAGGACCCC TCTGGTTCTA GTGGTATCGT TGTGCAGGGG 
    CTGACAGAAG ACATGATCAC CATTTTAATC CGTGCCTGTG TCAGCATGAT CAGTGTTCCT 
    GTGGACCCAG ACACTTTGCA CGCCACCTTG CGGCTCTGTC TGCGCCTCAC ACGCACACAC 
    CATTACGCCA TGATGTTTGC AGAGCTCAAG AGCACCCGCA TGATTCTGAA TCTCACCCAG 
    AGCTCAGGCT TTAATGGCTT CACCCCATTG GTCACCCTGC TCTTCCGCCA CATCATAGAG 
    GACCCGGCCA CACTGCGGCA CACTATGGAG AAGGTGGTAC GTTCTGCAGT CACCAGTGGT 
    GCAGGAAGCA CTACTTCAGG TGTGGTGTCA GGTAGTCTGG GCTCCAGGGA AATTAACTAC 
    ATCCTGCGAG TCCTTGGCCC AGCGGCCTGT CGCAATCCAG AGTGCTTTAC GGAAACTGCC 
    AAGAGTTGCA TCCGAATTGC TCTCCCGGCT CCAAGAGGTG CTGGCACTGC CTCAGATGAC 
    GAGTTTGAGA ACTTCAGAAT TAAAGGTCCA AATGCTGTGC AACTCGTGAA GACCACCCCC 
    CTAAAGCTCT CCCCGTTGCC CTCCATACCA GAGACTATAA AAGAGGTCAT CTATGACATG 
    CTGAATGCCC TGGCAGCATA TCACGCACCA GAAGAGGCAG AGAGAGGAGA GGAGCGGGCA 
    AACGCTGTAC CAGGGAATCA AGACCTGTGT CAGATCCTGC AGGATGTCGG TGATGATGTC 
    TACCAGCAGT ACAGACTCAC CAGACAAGGC AGTGATTTTG ACTCGCAATC TGCCTTCCCC 
    ATCAATACTC AGGTGTTTGC TGCAGATGGT GCTGTAGCTG AATCTTCTCA GTCTGGAACT 
    CCACAGGGAG AAGCCTCTAC ACCCGAGGAA ATGCGTGAAG AGAAAAAGGA GCAGGAAGGA 
    GACAAGGGAG CTTCTTCGGA GGAGAGCCGA GCTGCTAAGG CTAAAGCTAG CAAACCTCTG 
    ATGCCCACCT CTACCATCTT GAGGCTGTTG GCCGAGCTGG TTCGCTCTTA TGTTGGCATT 
    GCCACTCTTA TCACCTCTTA CAACTACACA GCTGGCCAGT CGGAGCTCAT TAAGGAAGAT 
    TGCAGTGTGT TGGCATTTGT TTTGGACCAC TTGCTCCCAC ACACCCAGAA CTCTGAAGAT 
    AAGGACACCC CTGCCCTTGC ACGCCTTTTC TTGGCCAGCC TTGCTGCAGC TGGCACGGGC 
    ACCGATGCCC AGGCTGCACT GGTCAACGAG GTCAAAGCTG CTTTGAGTCG AGCATTGGGC 
    ATGGCAGAGG GCACAGAGAA ACACGCCCGT TTGCAAGCAG TGATGTGCAT CATCAGCACC 
    ATTATGGAGT CGTGTCCTTC CACCTCCAGC TTCTACAGCA CAGCCACAGC TAAGACCCAG 
    CACAACGGCA TGAACAATAT CATCCGGCTC TTCTTGAAGA AAGGCCTTGT CAATGACCTG 
    GCCCGTGTGC CTCACAGCCT TGATCTATCC AGTCCCAATA TGGCAAACAC AGTAAATGCT 
    GCTTTGAAGC CTCTGGAAAC ATTGTCGCGA ATTGTGAACC AGCCCAGCAG CCTGTTTGGT 
    GGTAAAGGGG GATCCAGCAA GAACAAGACT GAACATGACA CTGTTGGCAC AGTCAGAGAC 
    AGTAACAGCA ACACTCAGGA TCAAGGAGAA GTGGGTGAGG CAGAGCCGGT TGAGAATCGT 
    GACCGAGGTC AAGCCACAGA CTCTGACCTG ATGGATGGAG AAGCCGAGGG AGACACTGTG 
    GTCATTGCTG GACAGCCTGA GGTGCTGAGC ACTCAGGCAA TGCAGGTGGA GAACGAGTTG 
    GTGGATTTGA TCGATGAACT TTTAGAGAGA GATGCCCAAA CTGTTAATAG TACCATCATC 
    GTAGGTCGTT CTGGAGAGGA TGAGTCTCAA GAGGATGTGC TGATGGATGA AGCTCCTTCC 
    AATATGAGTC AGGCCTCCAC TCTCCAAGCC AACAGAGAAG ACTCCATGAA CATCCTCGAG 
    CCAGAAGATG AGGAGCACAC GCAGGAGGAA GACTCCAGTG GTAGCAACGA GGATGAGGAC 
    AGTCAGGATG AAGAGGAAGA GGAGGAGGAA GAAGAGGAGG AAGATCAGGA TGATGAAGAG 
    GGAGATGAGG ATGATGACGA TGAAGGCTCA GAGATGGAAC TGGATGAAGA TTTTCCAGAT 
    ATCAATGCTG CCCCTCATAT TCGTTTTGAG CGATTCGACC GAGATGATGA CCTCATCATT 
    GAGTTTGATA ATATGTTCTC CACTTCAGCT GACATCCCTC CCTCACCAGG AAATATCCCC 
    AGCTCCCACC CTCTGATGGT GCGTCACGCT GACCATGGCT CGCTGACTCT TGGGGGATCG 
    GGCACCAGTA GCAGATTGGC TCAAGGAATG GGACGTAGTC AACGTTCTCT CCGCCAGCTC 
    ACGGCCAACA CAGGCCACAC GATCCATGTA CATTATCCTG GCAACCGTCA GCCTAACCCT 
    CCACTCATTC TGCAGAGATT ATTGGGCCCC AGCGCTGCAG CTGATATCTT GCAGCTGTCC 
    AGCTCTCTTC CTCTGCAGTC TCGAGGTCGT GCCCGTTTAA TGGTGGGTAA TGAAGATGTG 
    CACATTATTG CCCGTTCAGA CGATGAGCTG CTGGATGACT TCTTCCATGA GCAGAGCAGC 
    ACCGGAGGAC AAGCTGGCAC CCTTTCAAGT ATCCCGACTG CCTTAACCAG ATGGACAGAT 
    GAGTGTAAAG TGCTGGATGC TGAGAGCATG CATGACTGTG TGGCAGTGGT GAAGGTGCCC 
    ATACTGCAGC ACCTGGAGAG TTTGCGTGAT GAGGAGCTGG AGGAACGCAG GGAGAAAAGG 
    AGGAGGCAGT TGGCAGAGGA AGAGGAGACT GCAAAGCAGA ATGAGAGTTC TGCAGCAGGG 
    GAGGAGACCA GGGAACAGAG TCTGCAGGGC TCTGGGATTG GAACAGTCAA TGGAGCTGCA 
    GAGAATGCTG CAGAAGGAGA ACAGGCCCAG AGTGGGGTGT CCTCCTGTCT GGACCCTCCT 
    CGCACCTCTG AGGGTTTCCT CACAGCGCCC CCATCAGGAG AAGTCACTCC AACCACCCCT 
    GCCCCCCATG AACAAGCCCT CGTGTCTCTG GAAACAGCCA TCAGTCAGCA GGTGCACCAG 
    CCAATCACAG AGCTGCTGCT GGCCGACTCC AATCCTGACT CTCACGGAGC CTTGGCAGGG 
    GCAGGGCTTC CCCAGCTCTC AGCACCTGAT AGGCCAAATT GTGAGGCAGA AGCATCTCAG 
    ATGGAGATGT CACCTGCACC CACTATTGGT GAGAGAGGAG GAGGAGCATT GGATGCAGTC 
    AGGGAAGCTT CACTCAGTCC AGATATAGCT GAAAGTTCAG AGCCTGTGCC AGTGGGTGTG 
    TCTCAACTCG AGGGTTCGCC CATGGATACG AGCAGTCCCG CTTCAGGAAC GCAGGAAGAG 
    CCGGCTGCCA GCTCAGTTCC ATCCAATCAC ATGTCTCAGG AGCTATCAGG AAGTGGTGAC 
    AGCGGCCTCA CAGACAGGCA GACAGATGCA GAAACTGGAT CAACTTCTGT TTCATCTCCT 
    GGCGAGACCA TGCCTCGGAG TGACAGTGCC GATAGCCAGT CGCAGGCCAT CCAGGAGGAG 
    CCCCTCCCTT CCACCAGCAA TGAAGAGGAG GACCCTCTGG CAGGCATCAG TCTACCTGAA 
    GGCGTGGATC CGTCTTTCTT GGCAGCACTG CCTGAAGACA TTCGTCGGGA GGTGCTGCAG 
    AACCAACTGG GAATCAGACC TCCTTCTCGA CCTCCTGTCG CCTCCACTCT GACAACCACT 
    GCTAGTGCTG TGCTAGGAGG AGGGCCAGGG GTGACCGAAG TCAGCCCTGA ATTCTTAGCG 
    GCTCTCCCAC CTGCAATTCA GGAAGAGGTC CTGGCCCAGC AGCGGGCAGA GCAGCAGCGT 
    CGGGAGTTAT CCCAGCAACC CACACAGGGT GATCAACCTC TGGACCCAGT CACATTCATC 
    CAGACCCTGC CCTCTGAGCT GCGGCGCTCC GTTCTGGAAG ACATGGAGGA CAGTGTACTA 
    GCGGTCATGC CTCCAGATAT TGCAGCAGAG GCTGCAGCAC TGCGAAGGGA ACAAGAGGCC 
    AGACAGAGAC AACTGATGCA CGAGCGCTTG TTCGGTCACA GCTCCTCTTC AGCTCTGTCT 
    GCCATCTTAC GCTCTCCCGC CTTCACCAGC CGCCTCGGGG GCAACCGTGG GGTACAGTAC 
    ACCCGGCTGG CTGTGCAAAG AGGAGGGACG TTTCAAATGG GAGGGAGTGC CAATCATAGC 
    AGACCCTCTA GTTCAAACGT TGACTCTCTC CTGCGCCTGC GGGGGCGTCT CTTGTTGGAC 
    CACGAGGCCT TGTCCTGCCT TCTTGTGCTG CTTTTTGTGG ACGAGCCCAA ACTTAACACC 
    AGCCGCCTCC ACCGAGTGCT GCGTAACTTG TGCTATCACT CTCAGACGCG TGGCTGGGTC 
    ATCCGCAGTC TCCTGTCCAT CCTGCAGCGC AGCAGTGAGA GCGAGCTGTG CCTGGAGACT 
    GCACGCTTGG AGGAGTCCCG TGGCAAACGC TCACTAGGCC AGGGCTCATC TAGTGGAGGA 
    AAGGGTTGTT CTTCATCCTC CCAGTCGGCT ATAGCCTCTT CGTCATCGTC CTCCTCGTCC 
    TCTTCTTCTC TGGAGCTGCT GAACAGGGTG GAGTCACGGA GTTCCAGTCA GCTCTCCTGG 
    CTGTCTGTAT CAATGGACGC AGCTCTTGGA TGCAGAACCA ACATCTTTCA GATCCAGCGA 
    GTATCAGGCA GGAAACACGC AGAGAGGCAT TCAGCAGGAG GGAGTGTGAG CGCAGGAGGA 
    CTCACAGGTG GAGTGTCAGG AACAGGAATG ACGTGTACAG GGGGAGGTGG GTCAACTGTT 
    CACATCCATC CACAGGCTGC GCCGGTGGTC TGTCGACATG TACTGGACAC CCTCATCCAA 
    TTGGCCAAGG TATTTCCAAG TCACTTCACT CAACAACGCT GTAAAGATCC CCTATCCTCC 
    TCCTCCTCTG AGTTGGACAC TCGTCTTTGT GCCAGCTCAA CACCGACTAT GAGTGCCAGT 
    TGTCGGTCAG GTGGCACCAG TCAGAGTAAC TCCTCTGCTA CCCCAAGTAG TCTCACTTCT 
    ATCCACAATT CCCTGAGCGC CTGCTCTGGT ACGCCACAGT CCCTAGGCAT CTCCACCGAC 
    TTTTGGGATC TCCTGGTCAA GCTGGACAAC ATGAATGTGA GCCGAAAGGG GAAGGCCTCC 
    ATGAAGACTG TCCCTCTAGG GGGTGTCGGC GAGACAGAGG GTGCGCTGTT CAGCCTTGAG 
    GCGTCTCCGC TGGGCCAGCT GATGAATATG CTGTCCCACC CTGTGATCCG CCGTAGTTCC 
    CTCCTCACGG AGAAACTGCT ACGTCTGCTG TCTCTGATCT CCATCGCTCT GCCTGACAAC 
    AAGGCCACGG AGGTTCCAGC CAGCCATCCA GTGCCTCAGA CCCCAACTGC CACGCCATCT 
    GCACCTGCTC CTCAAACCAC AGCAGGGTCA GGGACCATCT CAGCAGTGGC AGCTACAGGC 
    ACTACGCAGG GTTCAGTCGG TTCAGTGGGT ACAACGCCAT CTCAACCAGC CGCTACAATA 
    TCTGTGGTTG CAGGCAGCAC CACCGCCTCT AGCATTATTG CTGGAAAACT GAAGCCAGCA 
    GTAAGCATCA CGGACATTGA ATCTAAACTG GCCTCTACTG GACTTACGGA GAAGCAGCTG 
    CAGCTCTCTG TGGAGGTTCT GACATCTCAC TCGTGTTCAG AGGAAGGTCT GGAGGACGCA 
    GCCAACATCT TGCTGCAGCT GTCCAGAGGG GACGCACCGA CCCGAGACAC TGTGCTTAGA 
    CTTCTGCTCA GCGGAGCACG TCACCTTGGA TACACTCTCT GCAAACAAAT CGGCACTTTA 
    TTAGCTGAGT TGAGGGAGTA TAATCTAGAG CAGCAGAGAA GAGCACGGGC TGATGCTCAG 
    TCTCCTGAAG CTTCTCATGA TGACCCATCC ATCACCACAC GCCTCAAGGG CAAGATGACC 
    AGCAGGTTTG ACGGGTCAGA GAGTGTGGTG ATTGTTGCTG CTCAGAAGCG CACGTTAGGT 
    GGCCGGGAGC TTCAGTTGCC CTGCATGAGC TCGCTCACTT CCAAGACCTC AACGCAGAAG 
    TTTTTCTTGC GGGTACTGCA GGTCATCATC CAGCTTAGAG AAGACACGCG GCGCGCTAAC 
    AAGAAAGCCA AGCAGACAGG AGGAAGACTA AGTTCAACCA GTCTGGGCTC GGCCAGTAGT 
    ATTCAGGCTG CGGTCCGTCA GCTGGAGGCT GAAGCGGACG CCATCATTCA GATGGTGAGA 
    GAGGGACAGA GGGCACGCCG TCTACAGCAG GCCCCCCCAC CCTCTTCATC TGCATCCTCT 
    TCCGCTGCTG CTGCTGCTGC ATCCGCTGCT GTGGCTGTGG CTGGATCGTC TGCCCCCAGT 
    ACCTCTGCAA CCAACAACCC CCCCGCTGGC ACGGCCAGCG CAGCCACGTC GGAGGTTCAC 
    ACTGTTGCCG AGCCACCAGC AGCTCAGAGA GATGATTCGC CCATGGATGT GGATCAGCCC 
    TCTCCTTTGG AACAGGACCA GCCAACATTA GGTGAGGAGA GCAGCAGTCA GCAGGAGCAG 
    GAGGAGCGTT TGCCTGACCT GCCGCTGTTA AGTGAGCAGC TGCTTTTGGA TGAGCTGTGG 
    GACATGCTTG GAGAATGTCT GAAAGAGCTT GAAGAGTCTC ATGACCAACA TGCAGTTCTA 
    GTGCTGCAGC CTGCAGTGGA GGCCTTTTTC TTGGTGCATG CAACAGAGCG GGAAAGCAAA 
    CCACCAGTTC GGGACACTCG GGAGAGCCAG CTGTCACACA TCAAGGATGA GCCTCCACCC 
    CTGTCACCCG CCCCTCTCAC ACCCGCTACC CCCTCCTCTC TGGACCCCTT TTTTTCCAGA 
    GAGCCTTCCT CTATGCACAT CTCTTCCAAC CTGCCTCCAG ATACACAGAA GTTCCTCCGT 
    TTCGCAGAGA CCCACCGCAC AGTGCTGAAT CAGATCCTGC GTCAATCCAC CACTCACCTG 
    GCAGACGGGC CTTTCGCTGT TTTGGTTGAC TACATTCGCA TCTTGGACTT TGACGTCAAG 
    CGCAAGTATT TCCGTCAGGA GCTGGAGCGT CTGGATGAAG GCTTGAGGAA AGAGGATATG 
    GCCGTCCATG TGAGGAGAGA TCACGTCTTT GAAGATTCTT ACAGAGAGCT GCACCGCAAG 
    AGCCCAGAAG ACATGAAGAA CAGATTGTAT ATAGTATTTG AAGGAGAGGA GGGTCAGGAT 
    GCAGGCGGGC TGTTAAGAGA GTGGTACATG ATCATCTCCA GAGAAATGTT CAACCCCATG 
    TATGCCCTGT TTCGCACCTC CCCCGGCGAT CGGGTCACCT ATACCATCAA CCCCTCCTCC 
    CACTGCAACC CCAACCATCT CAGCTACTTC AAGTTTGTGG GACGTGTGGT GGCCAAAGCA 
    GTCTACGACA ACAGGCTATT AGAGTGTTAC TTTACACGCA GCTTCTACAA GCACATCCTG 
    GGCAAGAGCG TTAGGTATAC AGACATGGAA AGCGAAGACT ATCCCTTTTT CCAAGGTCTG 
    GTTTATTTGT TGGAGAACGA TGTGTCGACT CTGGGCTACG AGCTGACATT CAGCACAGAG 
    GTCCAAGAGT TTGGCGTGTG TGAAGTGAGA GATCTGAAGG CCAACGGTGC CAACATTCTT 
    GTCACAGAAG AGAATAAGAA GGAATATGTG CATCTGGTCT GCCAGATGAA GATGACAGGT 
    GCAATCCGTA AACAGCTGGC TGCCTTCCTG GAGGGCTTTT ATGAGATCAT CCCCAAGAGA 
    CTGATCTCCA TCTTCACTGA GCAAGAGCTG GAGCTGCTCA TCTCCGGCCT GCCCACCATT 
    GACATCGACG ACCTCAAGGC CAATACTGAA TACCATAAAT ATCAGTCCAG TTCTATTCAG 
    ATCCAGTGGT TCTGGCGTGC TTTACGATCC TTCGACCAGG CTGACAGGGC CAAATTTCTG 
    CAGTTTGTTA CGGGCACTTC CAAGGTGCCA CTGCAGGGCT TTGCTGCACT GGAGGGAATG 
    AACGGCATTC AGAAGTTCCA GATCCACCGA GATGACCGCT CCACTGACCG CCTGCCCTCT 
    GCGCACACTT GCTTTAACCA GTTGGACCTC CCAGCGTACG AGAGCTACGA GAAGTTAAGA 
    CACATGCTGC TGCTGGCCAT TCAGGAATGT TCTGAAGGCT TCGGACTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_005162282.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162339.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID ODa27859
    Clone ID Related Accession (Same CDS sequence) XM_005162282.3 , XM_021473710.1 , XM_005162282.4
    Accession Version XM_005162282.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162282.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAAGCAGCT 
    CGCAGGCGTG AGGAGGAGGA GAGGAGAGCG AGGGAGCGGG TAGAGGAGGA AGAAGCTCGT 
    TGTCTGGAGC GCTTTCTGGA AGCAGAGCCT CTTGACACCA CTGAGCTTCA TGCCTTCACA 
    GACTCCATGC TGCCTGGCTG CTTCCACCTG CTCGATGAGC TGCCGGACAC AGTCTACCGC 
    CTCTGTGATC TGCTCATGAC TGCTATTAAA AGAAACGGCC CTGAATACAG AGACCTCATC 
    CTTAGACAAG TGGTCAATCA GGTGTGGGAG GCTGCTGACG TGTTGATAAA GGCTGCAGTG 
    CCGCTAACCA CCAGCGACAC CAAGACAGTC TCTGAGTGGA CCAGACAGAT GGCGACCCTC 
    CCTCAGGCCT CCAACCTGGC CACCCGCATC CTGCTGCTCA CGCTGCTCTT TGAGGAGCTG 
    AAACTCTCCT GCGCTCGGGT GGTGGAGAGC AGTGGTGTCT TGACTGTGCT GATTAAGCTG 
    TTGGAGGTGG TGCAGCCCTG TCTGCAAGCT GCCAAAGAGC AGAAGGACAT CCAAACGCCC 
    AAATGGATCA CCCCTGTGCT CCTACTTATT GACTTTTATG AGAAAATGGC TGTTTCATCC 
    AAACGAAGAG CTCAGATGAA CAAGTACCTC CAGCCCAATG GGAATAACTG GCGCTGGTTT 
    GATGACCGTT CAGGCCGCTG GTGCAGCTAC AGTGCAAGTA ACAACAGCAC TATTGACTCT 
    GCATGGAGGG CAGGAGAGAC CAGTGTCCGA TTTACAGCTG GCCGCAGAAG ATACACAGTT 
    CAGTTCAACA CCATGGTGCA GGTAAATGAG GAGACTGGCA ACAGACGACC AGTTATGTTG 
    ACGGTACAGC GGGTGCCACG CATTCCCAAG GCAACAAAAT CCGGAAGTGT TACTGACACG 
    GAGAGAGAGG AGAGTGAACA GAGAGCAAAG ACTGAGGAAA CACAGACTGA CACAGATTCC 
    AGCTCTGTGG CTGTAGAGAT GGCGCCCCCT AAAGAAGAAT CTGAGCTGAA GGATTCAACC 
    CCAGCAGCTC CGTCAGTTCT CCAGGACCCC TCTGGTTCTA GTGGTATCGT TGTGCAGGGG 
    CTGACAGAAG ACATGATCAC CATTTTAATC CGTGCCTGTG TCAGCATGAT CAGTGTTCCT 
    GTGGACCCAG ACACTTTGCA CGCCACCTTG CGGCTCTGTC TGCGCCTCAC ACGCACACAC 
    CATTACGCCA TGATGTTTGC AGAGCTCAAG AGCACCCGCA TGATTCTGAA TCTCACCCAG 
    AGCTCAGGCT TTAATGGCTT CACCCCATTG GTCACCCTGC TCTTCCGCCA CATCATAGAG 
    GACCCGGCCA CACTGCGGCA CACTATGGAG AAGGTGGTAC GTTCTGCAGT CACCAGTGGT 
    GCAGGAAGCA CTACTTCAGG TGTGGTGTCA GGTAGTCTGG GCTCCAGGGA AATTAACTAC 
    ATCCTGCGAG TCCTTGGCCC AGCGGCCTGT CGCAATCCAG AGTGCTTTAC GGAAACTGCC 
    AAGAGTTGCA TCCGAATTGC TCTCCCGGCT CCAAGAGGTG CTGGCACTGC CTCAGATGAC 
    GAGTTTGAGA ACTTCAGAAT TAAAGGTCCA AATGCTGTGC AACTCGTGAA GACCACCCCC 
    CTAAAGCTCT CCCCGTTGCC CTCCATACCA GAGACTATAA AAGAGGTCAT CTATGACATG 
    CTGAATGCCC TGGCAGCATA TCACGCACCA GAAGAGGCAG AGAGAGGAGA GGAGCGGGCA 
    AACGCTGTAC CAGGGAATCA AGACCTGTGT CAGATCCTGC AGGATGTCGG TGATGATGTC 
    TACCAGCAGT ACAGACTCAC CAGACAAGGC AGTGATTTTG ACTCGCAATC TGCCTTCCCC 
    ATCAATACTC AGGTGTTTGC TGCAGATGGT GCTGTAGCTG AATCTTCTCA GTCTGGAACT 
    CCACAGGGAG AAGCCTCTAC ACCCGAGGAA ATGCGTGAAG AGAAAAAGGA GCAGGAAGGA 
    GACAAGGGAG CTTCTTCGGA GGAGAGCCGA GCTGCTAAGG CTAAAGCTAG CAAACCTCTG 
    ATGCCCACCT CTACCATCTT GAGGCTGTTG GCCGAGCTGG TTCGCTCTTA TGTTGGCATT 
    GCCACTCTTA TCACCTCTTA CAACTACACA GCTGGCCAGT CGGAGCTCAT TAAGGAAGAT 
    TGCAGTGTGT TGGCATTTGT TTTGGACCAC TTGCTCCCAC ACACCCAGAA CTCTGAAGAT 
    AAGGACACCC CTGCCCTTGC ACGCCTTTTC TTGGCCAGCC TTGCTGCAGC TGGCACGGGC 
    ACCGATGCCC AGGCTGCACT GGTCAACGAG GTCAAAGCTG CTTTGAGTCG AGCATTGGGC 
    ATGGCAGAGG GCACAGAGAA ACACGCCCGT TTGCAAGCAG TGATGTGCAT CATCAGCACC 
    ATTATGGAGT CGTGTCCTTC CACCTCCAGC TTCTACAGCA CAGCCACAGC TAAGACCCAG 
    CACAACGGCA TGAACAATAT CATCCGGCTC TTCTTGAAGA AAGGCCTTGT CAATGACCTG 
    GCCCGTGTGC CTCACAGCCT TGATCTATCC AGTCCCAATA TGGCAAACAC AGTAAATGCT 
    GCTTTGAAGC CTCTGGAAAC ATTGTCGCGA ATTGTGAACC AGCCCAGCAG CCTGTTTGGT 
    GGTAAAGGGG GATCCAGCAA GAACAAGACT GAACATGACA CTGTTGGCAC AGTCAGAGAC 
    AGTAACAGCA ACACTCAGGA TCAAGGAGAA GTGGGTGAGG CAGAGCCGGT TGAGAATCGT 
    GACCGAGGTC AAGCCACAGA CTCTGACCTG ATGGATGGAG AAGCCGAGGG AGACACTGTG 
    GTCATTGCTG GACAGCCTGA GGTGCTGAGC ACTCAGGCAA TGCAGGTGGA GAACGAGTTG 
    GTGGATTTGA TCGATGAACT TTTAGAGAGA GATGCCCAAA CTGTTAATAG TACCATCATC 
    GTAGGTCGTT CTGGAGAGGA TGAGTCTCAA GAGGATGTGC TGATGGATGA AGCTCCTTCC 
    AATATGAGTC AGGCCTCCAC TCTCCAAGCC AACAGAGAAG ACTCCATGAA CATCCTCGAG 
    CCAGAAGATG AGGAGCACAC GCAGGAGGAA GACTCCAGTG GTAGCAACGA GGATGAGGAC 
    AGTCAGGATG AAGAGGAAGA GGAGGAGGAA GAAGAGGAGG AAGATCAGGA TGATGAAGAG 
    GGAGATGAGG ATGATGACGA TGAAGGCTCA GAGATGGAAC TGGATGAAGA TTTTCCAGAT 
    ATCAATGCTG CCCCTCATAT TCGTTTTGAG CGATTCGACC GAGATGATGA CCTCATCATT 
    GAGTTTGATA ATATGTTCTC CACTTCAGCT GACATCCCTC CCTCACCAGG AAATATCCCC 
    AGCTCCCACC CTCTGATGGT GCGTCACGCT GACCATGGCT CGCTGACTCT TGGGGGATCG 
    GGCACCAGTA GCAGATTGGC TCAAGGAATG GGACGTAGTC AACGTTCTCT CCGCCAGCTC 
    ACGGCCAACA CAGGCCACAC GATCCATGTA CATTATCCTG GCAACCGTCA GCCTAACCCT 
    CCACTCATTC TGCAGAGATT ATTGGGCCCC AGCGCTGCAG CTGATATCTT GCAGCTGTCC 
    AGCTCTCTTC CTCTGCAGTC TCGAGGTCGT GCCCGTTTAA TGGTGGGTAA TGAAGATGTG 
    CACATTATTG CCCGTTCAGA CGATGAGCTG CTGGATGACT TCTTCCATGA GCAGAGCAGC 
    ACCGGAGGAC AAGCTGGCAC CCTTTCAAGT ATCCCGACTG CCTTAACCAG ATGGACAGAT 
    GAGTGTAAAG TGCTGGATGC TGAGAGCATG CATGACTGTG TGGCAGTGGT GAAGGTGCCC 
    ATACTGCAGC ACCTGGAGAG TTTGCGTGAT GAGGAGCTGG AGGAACGCAG GGAGAAAAGG 
    AGGAGGCAGT TGGCAGAGGA AGAGGAGACT GCAAAGCAGA ATGAGAGTTC TGCAGCAGGG 
    GAGGAGACCA GGGAACAGAG TCTGCAGGGC TCTGGGATTG GAACAGTCAA TGGAGCTGCA 
    GAGAATGCTG CAGAAGGAGA ACAGGCCCAG AGTGGGGTGT CCTCCTGTCT GGACCCTCCT 
    CGCACCTCTG AGGGTTTCCT CACAGCGCCC CCATCAGGAG AAGTCACTCC AACCACCCCT 
    GCCCCCCATG AACAAGCCCT CGTGTCTCTG GAAACAGCCA TCAGTCAGCA GGTGCACCAG 
    CCAATCACAG AGCTGCTGCT GGCCGACTCC AATCCTGACT CTCACGGAGC CTTGGCAGGG 
    GCAGGGCTTC CCCAGCTCTC AGCACCTGAT AGGCCAAATT GTGAGGCAGA AGCATCTCAG 
    ATGGAGATGT CACCTGCACC CACTATTGGT GAGAGAGGAG GAGGAGCATT GGATGCAGTC 
    AGGGAAGCTT CACTCAGTCC AGATATAGCT GAAAGTTCAG AGCCTGTGCC AGTGGGTGTG 
    TCTCAACTCG AGGGTTCGCC CATGGATACG AGCAGTCCCG CTTCAGGAAC GCAGGAAGAG 
    CCGGCTGCCA GCTCAGTTCC ATCCAATCAC ATGTCTCAGG AGCTATCAGG AAGTGGTGAC 
    AGCGGCCTCA CAGACAGGCA GACAGATGCA GAAACTGGAT CAACTTCTGT TTCATCTCCT 
    GGCGAGACCA TGCCTCGGAG TGACAGTGCC GATAGCCAGT CGCAGGCCAT CCAGGAGGAG 
    CCCCTCCCTT CCACCAGCAA TGAAGAGGAG GACCCTCTGG CAGGCATCAG TCTACCTGAA 
    GGCGTGGATC CGTCTTTCTT GGCAGCACTG CCTGAAGACA TTCGTCGGGA GGTGCTGCAG 
    AACCAACTGG GAATCAGACC TCCTTCTCGA CCTCCTGTCG CCTCCACTCT GACAACCACT 
    GCTAGTGCTG TGCTAGGAGG AGGGCCAGGG GTGACCGAAG TCAGCCCTGA ATTCTTAGCG 
    GCTCTCCCAC CTGCAATTCA GGAAGAGGTC CTGGCCCAGC AGCGGGCAGA GCAGCAGCGT 
    CGGGAGTTAT CCCAGCAACC CACACAGGGT GATCAACCTC TGGACCCAGT CACATTCATC 
    CAGACCCTGC CCTCTGAGCT GCGGCGCTCC GTTCTGGAAG ACATGGAGGA CAGTGTACTA 
    GCGGTCATGC CTCCAGATAT TGCAGCAGAG GCTGCAGCAC TGCGAAGGGA ACAAGAGGCC 
    AGACAGAGAC AACTGATGCA CGAGCGCTTG TTCGGTCACA GCTCCTCTTC AGCTCTGTCT 
    GCCATCTTAC GCTCTCCCGC CTTCACCAGC CGCCTCGGGG GCAACCGTGG GGTACAGTAC 
    ACCCGGCTGG CTGTGCAAAG AGGAGGGACG TTTCAAATGG GAGGGAGTGC CAATCATAGC 
    AGACCCTCTA GTTCAAACGT TGACTCTCTC CTGCGCCTGC GGGGGCGTCT CTTGTTGGAC 
    CACGAGGCCT TGTCCTGCCT TCTTGTGCTG CTTTTTGTGG ACGAGCCCAA ACTTAACACC 
    AGCCGCCTCC ACCGAGTGCT GCGTAACTTG TGCTATCACT CTCAGACGCG TGGCTGGGTC 
    ATCCGCAGTC TCCTGTCCAT CCTGCAGCGC AGCAGTGAGA GCGAGCTGTG CCTGGAGACT 
    GCACGCTTGG AGGAGTCCCG TGGCAAACGC TCACTAGGCC AGGGCTCATC TAGTGGAGGA 
    AAGGGTTGTT CTTCATCCTC CCAGTCGGCT ATAGCCTCTT CGTCATCGTC CTCCTCGTCC 
    TCTTCTTCTC TGGAGCTGCT GAACAGGGTG GAGTCACGGA GTTCCAGTCA GCTCTCCTGG 
    CTGTCTGTAT CAATGGACGC AGCTCTTGGA TGCAGAACCA ACATCTTTCA GATCCAGCGA 
    GTATCAGGCA GGAAACACGC AGAGAGGCAT TCAGCAGGAG GGAGTGTGAG CGCAGGAGGA 
    CTCACAGGTG GAGTGTCAGG AACAGGAATG ACGTGTACAG GGGGAGGTGG GTCAACTGTT 
    CACATCCATC CACAGGCTGC GCCGGTGGTC TGTCGACATG TACTGGACAC CCTCATCCAA 
    TTGGCCAAGG TATTTCCAAG TCACTTCACT CAACAACGCT GTAAAGATCC CCTATCCTCC 
    TCCTCCTCTG AGTTGGACAC TCGTCTTTGT GCCAGCTCAA CACCGACTAT GAGTGCCAGT 
    TGTCGGTCAG GTGGCACCAG TCAGAGTAAC TCCTCTGCTA CCCCAAGTAG TCTCACTTCT 
    ATCCACAATT CCCTGAGCGC CTGCTCTGGT ACGCCACAGT CCCTAGGCAT CTCCACCGAC 
    TTTTGGGATC TCCTGGTCAA GCTGGACAAC ATGAATGTGA GCCGAAAGGG GAAGGCCTCC 
    ATGAAGACTG TCCCTCTAGG GGGTGTCGGC GAGACAGAGG GTGCGCTGTT CAGCCTTGAG 
    GCGTCTCCGC TGGGCCAGCT GATGAATATG CTGTCCCACC CTGTGATCCG CCGTAGTTCC 
    CTCCTCACGG AGAAACTGCT ACGTCTGCTG TCTCTGATCT CCATCGCTCT GCCTGACAAC 
    AAGGCCACGG AGGTTCCAGC CAGCCATCCA GTGCCTCAGA CCCCAACTGC CACGCCATCT 
    GCACCTGCTC CTCAAACCAC AGCAGGGTCA GGGACCATCT CAGCAGTGGC AGCTACAGGC 
    ACTACGCAGG GTTCAGTCGG TTCAGTGGGT ACAACGCCAT CTCAACCAGC CGCTACAATA 
    TCTGTGGTTG CAGGCAGCAC CACCGCCTCT AGCATTATTG CTGGAAAACT GAAGCCAGCA 
    GTAAGCATCA CGGACATTGA ATCTAAACTG GCCTCTACTG GACTTACGGA GAAGCAGCTG 
    CAGCTCTCTG TGGAGGTTCT GACATCTCAC TCGTGTTCAG AGGAAGGTCT GGAGGACGCA 
    GCCAACATCT TGCTGCAGCT GTCCAGAGGG GACGCACCGA CCCGAGACAC TGTGCTTAGA 
    CTTCTGCTCA GCGGAGCACG TCACCTTGGA TACACTCTCT GCAAACAAAT CGGCACTTTA 
    TTAGCTGAGT TGAGGGAGTA TAATCTAGAG CAGCAGAGAA GAGCACGGGC TGATGCTCAG 
    TCTCCTGAAG CTTCTCATGA TGACCCATCC ATCACCACAC GCCTCAAGGG CAAGATGACC 
    AGCAGGTTTG ACGGGTCAGA GAGTGTGGTG ATTGTTGCTG CTCAGAAGCG CACGTTAGGT 
    GGCCGGGAGC TTCAGTTGCC CTGCATGAGC TCGCTCACTT CCAAGACCTC AACGCAGAAG 
    TTTTTCTTGC GGGTACTGCA GGTCATCATC CAGCTTAGAG AAGACACGCG GCGCGCTAAC 
    AAGAAAGCCA AGCAGACAGG AGGAAGACTA AGTTCAACCA GTCTGGGCTC GGCCAGTAGT 
    ATTCAGGCTG CGGTCCGTCA GCTGGAGGCT GAAGCGGACG CCATCATTCA GATGGTGAGA 
    GAGGGACAGA GGGCACGCCG TCTACAGCAG GCCCCCCCAC CCTCTTCATC TGCATCCTCT 
    TCCGCTGCTG CTGCTGCTGC ATCCGCTGCT GTGGCTGTGG CTGGATCGTC TGCCCCCAGT 
    ACCTCTGCAA CCAACAACCC CCCCGCTGGC ACGGCCAGCG CAGCCACGTC GGAGGTTCAC 
    ACTGTTGCCG AGCCACCAGC AGCTCAGAGA GATGATTCGC CCATGGATGT GGATCAGCCC 
    TCTCCTTTGG AACAGGACCA GCCAACATTA GGTGAGGAGA GCAGCAGTCA GCAGGAGCAG 
    GAGGAGCGTT TGCCTGACCT GCCGCTGTTA AGTGAGCAGC TGCTTTTGGA TGAGCTGTGG 
    GACATGCTTG GAGAATGTCT GAAAGAGCTT GAAGAGTCTC ATGACCAACA TGCAGTTCTA 
    GTGCTGCAGC CTGCAGTGGA GGCCTTTTTC TTGGTGCATG CAACAGAGCG GGAAAGCAAA 
    CCACCAGTTC GGGACACTCG GGAGAGCCAG CTGTCACACA TCAAGGATGA GCCTCCACCC 
    CTGTCACCCG CCCCTCTCAC ACCCGCTACC CCCTCCTCTC TGGACCCCTT TTTTTCCAGA 
    GAGCCTTCCT CTATGCACAT CTCTTCCAAC CTGCCTCCAG ATACACAGAA GTTCCTCCGT 
    TTCGCAGAGA CCCACCGCAC AGTGCTGAAT CAGATCCTGC GTCAATCCAC CACTCACCTG 
    GCAGACGGGC CTTTCGCTGT TTTGGTTGAC TACATTCGCA TCTTGGACTT TGACGTCAAG 
    CGCAAGTATT TCCGTCAGGA GCTGGAGCGT CTGGATGAAG GCTTGAGGAA AGAGGATATG 
    GCCGTCCATG TGAGGAGAGA TCACGTCTTT GAAGATTCTT ACAGAGAGCT GCACCGCAAG 
    AGCCCAGAAG ACATGAAGAA CAGATTGTAT ATAGTATTTG AAGGAGAGGA GGGTCAGGAT 
    GCAGGCGGGC TGTTAAGAGA GTGGTACATG ATCATCTCCA GAGAAATGTT CAACCCCATG 
    TATGCCCTGT TTCGCACCTC CCCCGGCGAT CGGGTCACCT ATACCATCAA CCCCTCCTCC 
    CACTGCAACC CCAACCATCT CAGCTACTTC AAGTTTGTGG GACGTGTGGT GGCCAAAGCA 
    GTCTACGACA ACAGGCTATT AGAGTGTTAC TTTACACGCA GCTTCTACAA GCACATCCTG 
    GGCAAGAGCG TTAGGTATAC AGACATGGAA AGCGAAGACT ATCCCTTTTT CCAAGGTCTG 
    GTTTATTTGT TGGAGAACGA TGTGTCGACT CTGGGCTACG AGCTGACATT CAGCACAGAG 
    GTCCAAGAGT TTGGCGTGTG TGAAGTGAGA GATCTGAAGG CCAACGGTGC CAACATTCTT 
    GTCACAGAAG AGAATAAGAA GGAATATGTG CATCTGGTCT GCCAGATGAA GATGACAGGT 
    GCAATCCGTA AACAGCTGGC TGCCTTCCTG GAGGGCTTTT ATGAGATCAT CCCCAAGAGA 
    CTGATCTCCA TCTTCACTGA GCAAGAGCTG GAGCTGCTCA TCTCCGGCCT GCCCACCATT 
    GACATCGACG ACCTCAAGGC CAATACTGAA TACCATAAAT ATCAGTCCAG TTCTATTCAG 
    ATCCAGTGGT TCTGGCGTGC TTTACGATCC TTCGACCAGG CTGACAGGGC CAAATTTCTG 
    CAGTTTGTTA CGGGCACTTC CAAGGTGCCA CTGCAGGGCT TTGCTGCACT GGAGGGAATG 
    AACGGCATTC AGAAGTTCCA GATCCACCGA GATGACCGCT CCACTGACCG CCTGCCCTCT 
    GCGCACACTT GCTTTAACCA GTTGGACCTC CCAGCGTACG AGAGCTACGA GAAGTTAAGA 
    CACATGCTGC TGCTGGCCAT TCAGGAATGT TCTGAAGGCT TCGGACTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162339.1
    CDS142..13755
    Translation

    Target ORF information:

    RefSeq Version XM_005162282.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005162282.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    13561
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAAGCAGCT 
    CGCAGGCGTG AGGAGGAGGA GAGGAGAGCG AGGGAGCGGG TAGAGGAGGA AGAAGCTCGT 
    TGTCTGGAGC GCTTTCTGGA AGCAGAGCCT CTTGACACCA CTGAGCTTCA TGCCTTCACA 
    GACTCCATGC TGCCTGGCTG CTTCCACCTG CTCGATGAGC TGCCGGACAC AGTCTACCGC 
    CTCTGTGATC TGCTCATGAC TGCTATTAAA AGAAACGGCC CTGAATACAG AGACCTCATC 
    CTTAGACAAG TGGTCAATCA GGTGTGGGAG GCTGCTGACG TGTTGATAAA GGCTGCAGTG 
    CCGCTAACCA CCAGCGACAC CAAGACAGTC TCTGAGTGGA CCAGACAGAT GGCGACCCTC 
    CCTCAGGCCT CCAACCTGGC CACCCGCATC CTGCTGCTCA CGCTGCTCTT TGAGGAGCTG 
    AAACTCTCCT GCGCTCGGGT GGTGGAGAGC AGTGGTGTCT TGACTGTGCT GATTAAGCTG 
    TTGGAGGTGG TGCAGCCCTG TCTGCAAGCT GCCAAAGAGC AGAAGGACAT CCAAACGCCC 
    AAATGGATCA CCCCTGTGCT CCTACTTATT GACTTTTATG AGAAAATGGC TGTTTCATCC 
    AAACGAAGAG CTCAGATGAA CAAGTACCTC CAGCCCAATG GGAATAACTG GCGCTGGTTT 
    GATGACCGTT CAGGCCGCTG GTGCAGCTAC AGTGCAAGTA ACAACAGCAC TATTGACTCT 
    GCATGGAGGG CAGGAGAGAC CAGTGTCCGA TTTACAGCTG GCCGCAGAAG ATACACAGTT 
    CAGTTCAACA CCATGGTGCA GGTAAATGAG GAGACTGGCA ACAGACGACC AGTTATGTTG 
    ACGGTACAGC GGGTGCCACG CATTCCCAAG GCAACAAAAT CCGGAAGTGT TACTGACACG 
    GAGAGAGAGG AGAGTGAACA GAGAGCAAAG ACTGAGGAAA CACAGACTGA CACAGATTCC 
    AGCTCTGTGG CTGTAGAGAT GGCGCCCCCT AAAGAAGAAT CTGAGCTGAA GGATTCAACC 
    CCAGCAGCTC CGTCAGTTCT CCAGGACCCC TCTGGTTCTA GTGGTATCGT TGTGCAGGGG 
    CTGACAGAAG ACATGATCAC CATTTTAATC CGTGCCTGTG TCAGCATGAT CAGTGTTCCT 
    GTGGACCCAG ACACTTTGCA CGCCACCTTG CGGCTCTGTC TGCGCCTCAC ACGCACACAC 
    CATTACGCCA TGATGTTTGC AGAGCTCAAG AGCACCCGCA TGATTCTGAA TCTCACCCAG 
    AGCTCAGGCT TTAATGGCTT CACCCCATTG GTCACCCTGC TCTTCCGCCA CATCATAGAG 
    GACCCGGCCA CACTGCGGCA CACTATGGAG AAGGTGGTAC GTTCTGCAGT CACCAGTGGT 
    GCAGGAAGCA CTACTTCAGG TGTGGTGTCA GGTAGTCTGG GCTCCAGGGA AATTAACTAC 
    ATCCTGCGAG TCCTTGGCCC AGCGGCCTGT CGCAATCCAG AGTGCTTTAC GGAAACTGCC 
    AAGAGTTGCA TCCGAATTGC TCTCCCGGCT CCAAGAGGTG CTGGCACTGC CTCAGATGAC 
    GAGTTTGAGA ACTTCAGAAT TAAAGGTCCA AATGCTGTGC AACTCGTGAA GACCACCCCC 
    CTAAAGCTCT CCCCGTTGCC CTCCATACCA GAGACTATAA AAGAGGTCAT CTATGACATG 
    CTGAATGCCC TGGCAGCATA TCACGCACCA GAAGAGGCAG AGAGAGGAGA GGAGCGGGCA 
    AACGCTGTAC CAGGGAATCA AGACCTGTGT CAGATCCTGC AGGATGTCGG TGATGATGTC 
    TACCAGCAGT ACAGACTCAC CAGACAAGGC AGTGATTTTG ACTCGCAATC TGCCTTCCCC 
    ATCAATACTC AGGTGTTTGC TGCAGATGGT GCTGTAGCTG AATCTTCTCA GTCTGGAACT 
    CCACAGGGAG AAGCCTCTAC ACCCGAGGAA ATGCGTGAAG AGAAAAAGGA GCAGGAAGGA 
    GACAAGGGAG CTTCTTCGGA GGAGAGCCGA GCTGCTAAGG CTAAAGCTAG CAAACCTCTG 
    ATGCCCACCT CTACCATCTT GAGGCTGTTG GCCGAGCTGG TTCGCTCTTA TGTTGGCATT 
    GCCACTCTTA TCACCTCTTA CAACTACACA GCTGGCCAGT CGGAGCTCAT TAAGGAAGAT 
    TGCAGTGTGT TGGCATTTGT TTTGGACCAC TTGCTCCCAC ACACCCAGAA CTCTGAAGAT 
    AAGGACACCC CTGCCCTTGC ACGCCTTTTC TTGGCCAGCC TTGCTGCAGC TGGCACGGGC 
    ACCGATGCCC AGGCTGCACT GGTCAACGAG GTCAAAGCTG CTTTGAGTCG AGCATTGGGC 
    ATGGCAGAGG GCACAGAGAA ACACGCCCGT TTGCAAGCAG TGATGTGCAT CATCAGCACC 
    ATTATGGAGT CGTGTCCTTC CACCTCCAGC TTCTACAGCA CAGCCACAGC TAAGACCCAG 
    CACAACGGCA TGAACAATAT CATCCGGCTC TTCTTGAAGA AAGGCCTTGT CAATGACCTG 
    GCCCGTGTGC CTCACAGCCT TGATCTATCC AGTCCCAATA TGGCAAACAC AGTAAATGCT 
    GCTTTGAAGC CTCTGGAAAC ATTGTCGCGA ATTGTGAACC AGCCCAGCAG CCTGTTTGGT 
    GGTAAAGGGG GATCCAGCAA GAACAAGACT GAACATGACA CTGTTGGCAC AGTCAGAGAC 
    AGTAACAGCA ACACTCAGGA TCAAGGAGAA GTGGGTGAGG CAGAGCCGGT TGAGAATCGT 
    GACCGAGGTC AAGCCACAGA CTCTGACCTG ATGGATGGAG AAGCCGAGGG AGACACTGTG 
    GTCATTGCTG GACAGCCTGA GGTGCTGAGC ACTCAGGCAA TGCAGGTGGA GAACGAGTTG 
    GTGGATTTGA TCGATGAACT TTTAGAGAGA GATGCCCAAA CTGTTAATAG TACCATCATC 
    GTAGGTCGTT CTGGAGAGGA TGAGTCTCAA GAGGATGTGC TGATGGATGA AGCTCCTTCC 
    AATATGAGTC AGGCCTCCAC TCTCCAAGCC AACAGAGAAG ACTCCATGAA CATCCTCGAG 
    CCAGAAGATG AGGAGCACAC GCAGGAGGAA GACTCCAGTG GTAGCAACGA GGATGAGGAC 
    AGTCAGGATG AAGAGGAAGA GGAGGAGGAA GAAGAGGAGG AAGATCAGGA TGATGAAGAG 
    GGAGATGAGG ATGATGACGA TGAAGGCTCA GAGATGGAAC TGGATGAAGA TTTTCCAGAT 
    ATCAATGCTG CCCCTCATAT TCGTTTTGAG CGATTCGACC GAGATGATGA CCTCATCATT 
    GAGTTTGATA ATATGTTCTC CACTTCAGCT GACATCCCTC CCTCACCAGG AAATATCCCC 
    AGCTCCCACC CTCTGATGGT GCGTCACGCT GACCATGGCT CGCTGACTCT TGGGGGATCG 
    GGCACCAGTA GCAGATTGGC TCAAGGAATG GGACGTAGTC AACGTTCTCT CCGCCAGCTC 
    ACGGCCAACA CAGGCCACAC GATCCATGTA CATTATCCTG GCAACCGTCA GCCTAACCCT 
    CCACTCATTC TGCAGAGATT ATTGGGCCCC AGCGCTGCAG CTGATATCTT GCAGCTGTCC 
    AGCTCTCTTC CTCTGCAGTC TCGAGGTCGT GCCCGTTTAA TGGTGGGTAA TGAAGATGTG 
    CACATTATTG CCCGTTCAGA CGATGAGCTG CTGGATGACT TCTTCCATGA GCAGAGCAGC 
    ACCGGAGGAC AAGCTGGCAC CCTTTCAAGT ATCCCGACTG CCTTAACCAG ATGGACAGAT 
    GAGTGTAAAG TGCTGGATGC TGAGAGCATG CATGACTGTG TGGCAGTGGT GAAGGTGCCC 
    ATACTGCAGC ACCTGGAGAG TTTGCGTGAT GAGGAGCTGG AGGAACGCAG GGAGAAAAGG 
    AGGAGGCAGT TGGCAGAGGA AGAGGAGACT GCAAAGCAGA ATGAGAGTTC TGCAGCAGGG 
    GAGGAGACCA GGGAACAGAG TCTGCAGGGC TCTGGGATTG GAACAGTCAA TGGAGCTGCA 
    GAGAATGCTG CAGAAGGAGA ACAGGCCCAG AGTGGGGTGT CCTCCTGTCT GGACCCTCCT 
    CGCACCTCTG AGGGTTTCCT CACAGCGCCC CCATCAGGAG AAGTCACTCC AACCACCCCT 
    GCCCCCCATG AACAAGCCCT CGTGTCTCTG GAAACAGCCA TCAGTCAGCA GGTGCACCAG 
    CCAATCACAG AGCTGCTGCT GGCCGACTCC AATCCTGACT CTCACGGAGC CTTGGCAGGG 
    GCAGGGCTTC CCCAGCTCTC AGCACCTGAT AGGCCAAATT GTGAGGCAGA AGCATCTCAG 
    ATGGAGATGT CACCTGCACC CACTATTGGT GAGAGAGGAG GAGGAGCATT GGATGCAGTC 
    AGGGAAGCTT CACTCAGTCC AGATATAGCT GAAAGTTCAG AGCCTGTGCC AGTGGGTGTG 
    TCTCAACTCG AGGGTTCGCC CATGGATACG AGCAGTCCCG CTTCAGGAAC GCAGGAAGAG 
    CCGGCTGCCA GCTCAGTTCC ATCCAATCAC ATGTCTCAGG AGCTATCAGG AAGTGGTGAC 
    AGCGGCCTCA CAGACAGGCA GACAGATGCA GAAACTGGAT CAACTTCTGT TTCATCTCCT 
    GGCGAGACCA TGCCTCGGAG TGACAGTGCC GATAGCCAGT CGCAGGCCAT CCAGGAGGAG 
    CCCCTCCCTT CCACCAGCAA TGAAGAGGAG GACCCTCTGG CAGGCATCAG TCTACCTGAA 
    GGCGTGGATC CGTCTTTCTT GGCAGCACTG CCTGAAGACA TTCGTCGGGA GGTGCTGCAG 
    AACCAACTGG GAATCAGACC TCCTTCTCGA CCTCCTGTCG CCTCCACTCT GACAACCACT 
    GCTAGTGCTG TGCTAGGAGG AGGGCCAGGG GTGACCGAAG TCAGCCCTGA ATTCTTAGCG 
    GCTCTCCCAC CTGCAATTCA GGAAGAGGTC CTGGCCCAGC AGCGGGCAGA GCAGCAGCGT 
    CGGGAGTTAT CCCAGCAACC CACACAGGGT GATCAACCTC TGGACCCAGT CACATTCATC 
    CAGACCCTGC CCTCTGAGCT GCGGCGCTCC GTTCTGGAAG ACATGGAGGA CAGTGTACTA 
    GCGGTCATGC CTCCAGATAT TGCAGCAGAG GCTGCAGCAC TGCGAAGGGA ACAAGAGGCC 
    AGACAGAGAC AACTGATGCA CGAGCGCTTG TTCGGTCACA GCTCCTCTTC AGCTCTGTCT 
    GCCATCTTAC GCTCTCCCGC CTTCACCAGC CGCCTCGGGG GCAACCGTGG GGTACAGTAC 
    ACCCGGCTGG CTGTGCAAAG AGGAGGGACG TTTCAAATGG GAGGGAGTGC CAATCATAGC 
    AGACCCTCTA GTTCAAACGT TGACTCTCTC CTGCGCCTGC GGGGGCGTCT CTTGTTGGAC 
    CACGAGGCCT TGTCCTGCCT TCTTGTGCTG CTTTTTGTGG ACGAGCCCAA ACTTAACACC 
    AGCCGCCTCC ACCGAGTGCT GCGTAACTTG TGCTATCACT CTCAGACGCG TGGCTGGGTC 
    ATCCGCAGTC TCCTGTCCAT CCTGCAGCGC AGCAGTGAGA GCGAGCTGTG CCTGGAGACT 
    GCACGCTTGG AGGAGTCCCG TGGCAAACGC TCACTAGGCC AGGGCTCATC TAGTGGAGGA 
    AAGGGTTGTT CTTCATCCTC CCAGTCGGCT ATAGCCTCTT CGTCATCGTC CTCCTCGTCC 
    TCTTCTTCTC TGGAGCTGCT GAACAGGGTG GAGTCACGGA GTTCCAGTCA GCTCTCCTGG 
    CTGTCTGTAT CAATGGACGC AGCTCTTGGA TGCAGAACCA ACATCTTTCA GATCCAGCGA 
    GTATCAGGCA GGAAACACGC AGAGAGGCAT TCAGCAGGAG GGAGTGTGAG CGCAGGAGGA 
    CTCACAGGTG GAGTGTCAGG AACAGGAATG ACGTGTACAG GGGGAGGTGG GTCAACTGTT 
    CACATCCATC CACAGGCTGC GCCGGTGGTC TGTCGACATG TACTGGACAC CCTCATCCAA 
    TTGGCCAAGG TATTTCCAAG TCACTTCACT CAACAACGCT GTAAAGATCC CCTATCCTCC 
    TCCTCCTCTG AGTTGGACAC TCGTCTTTGT GCCAGCTCAA CACCGACTAT GAGTGCCAGT 
    TGTCGGTCAG GTGGCACCAG TCAGAGTAAC TCCTCTGCTA CCCCAAGTAG TCTCACTTCT 
    ATCCACAATT CCCTGAGCGC CTGCTCTGGT ACGCCACAGT CCCTAGGCAT CTCCACCGAC 
    TTTTGGGATC TCCTGGTCAA GCTGGACAAC ATGAATGTGA GCCGAAAGGG GAAGGCCTCC 
    ATGAAGACTG TCCCTCTAGG GGGTGTCGGC GAGACAGAGG GTGCGCTGTT CAGCCTTGAG 
    GCGTCTCCGC TGGGCCAGCT GATGAATATG CTGTCCCACC CTGTGATCCG CCGTAGTTCC 
    CTCCTCACGG AGAAACTGCT ACGTCTGCTG TCTCTGATCT CCATCGCTCT GCCTGACAAC 
    AAGGCCACGG AGGTTCCAGC CAGCCATCCA GTGCCTCAGA CCCCAACTGC CACGCCATCT 
    GCACCTGCTC CTCAAACCAC AGCAGGGTCA GGGACCATCT CAGCAGTGGC AGCTACAGGC 
    ACTACGCAGG GTTCAGTCGG TTCAGTGGGT ACAACGCCAT CTCAACCAGC CGCTACAATA 
    TCTGTGGTTG CAGGCAGCAC CACCGCCTCT AGCATTATTG CTGGAAAACT GAAGCCAGCA 
    GTAAGCATCA CGGACATTGA ATCTAAACTG GCCTCTACTG GACTTACGGA GAAGCAGCTG 
    CAGCTCTCTG TGGAGGTTCT GACATCTCAC TCGTGTTCAG AGGAAGGTCT GGAGGACGCA 
    GCCAACATCT TGCTGCAGCT GTCCAGAGGG GACGCACCGA CCCGAGACAC TGTGCTTAGA 
    CTTCTGCTCA GCGGAGCACG TCACCTTGGA TACACTCTCT GCAAACAAAT CGGCACTTTA 
    TTAGCTGAGT TGAGGGAGTA TAATCTAGAG CAGCAGAGAA GAGCACGGGC TGATGCTCAG 
    TCTCCTGAAG CTTCTCATGA TGACCCATCC ATCACCACAC GCCTCAAGGG CAAGATGACC 
    AGCAGGTTTG ACGGGTCAGA GAGTGTGGTG ATTGTTGCTG CTCAGAAGCG CACGTTAGGT 
    GGCCGGGAGC TTCAGTTGCC CTGCATGAGC TCGCTCACTT CCAAGACCTC AACGCAGAAG 
    TTTTTCTTGC GGGTACTGCA GGTCATCATC CAGCTTAGAG AAGACACGCG GCGCGCTAAC 
    AAGAAAGCCA AGCAGACAGG AGGAAGACTA AGTTCAACCA GTCTGGGCTC GGCCAGTAGT 
    ATTCAGGCTG CGGTCCGTCA GCTGGAGGCT GAAGCGGACG CCATCATTCA GATGGTGAGA 
    GAGGGACAGA GGGCACGCCG TCTACAGCAG GCCCCCCCAC CCTCTTCATC TGCATCCTCT 
    TCCGCTGCTG CTGCTGCTGC ATCCGCTGCT GTGGCTGTGG CTGGATCGTC TGCCCCCAGT 
    ACCTCTGCAA CCAACAACCC CCCCGCTGGC ACGGCCAGCG CAGCCACGTC GGAGGTTCAC 
    ACTGTTGCCG AGCCACCAGC AGCTCAGAGA GATGATTCGC CCATGGATGT GGATCAGCCC 
    TCTCCTTTGG AACAGGACCA GCCAACATTA GGTGAGGAGA GCAGCAGTCA GCAGGAGCAG 
    GAGGAGCGTT TGCCTGACCT GCCGCTGTTA AGTGAGCAGC TGCTTTTGGA TGAGCTGTGG 
    GACATGCTTG GAGAATGTCT GAAAGAGCTT GAAGAGTCTC ATGACCAACA TGCAGTTCTA 
    GTGCTGCAGC CTGCAGTGGA GGCCTTTTTC TTGGTGCATG CAACAGAGCG GGAAAGCAAA 
    CCACCAGTTC GGGACACTCG GGAGAGCCAG CTGTCACACA TCAAGGATGA GCCTCCACCC 
    CTGTCACCCG CCCCTCTCAC ACCCGCTACC CCCTCCTCTC TGGACCCCTT TTTTTCCAGA 
    GAGCCTTCCT CTATGCACAT CTCTTCCAAC CTGCCTCCAG ATACACAGAA GTTCCTCCGT 
    TTCGCAGAGA CCCACCGCAC AGTGCTGAAT CAGATCCTGC GTCAATCCAC CACTCACCTG 
    GCAGACGGGC CTTTCGCTGT TTTGGTTGAC TACATTCGCA TCTTGGACTT TGACGTCAAG 
    CGCAAGTATT TCCGTCAGGA GCTGGAGCGT CTGGATGAAG GCTTGAGGAA AGAGGATATG 
    GCCGTCCATG TGAGGAGAGA TCACGTCTTT GAAGATTCTT ACAGAGAGCT GCACCGCAAG 
    AGCCCAGAAG ACATGAAGAA CAGATTGTAT ATAGTATTTG AAGGAGAGGA GGGTCAGGAT 
    GCAGGCGGGC TGTTAAGAGA GTGGTACATG ATCATCTCCA GAGAAATGTT CAACCCCATG 
    TATGCCCTGT TTCGCACCTC CCCCGGCGAT CGGGTCACCT ATACCATCAA CCCCTCCTCC 
    CACTGCAACC CCAACCATCT CAGCTACTTC AAGTTTGTGG GACGTGTGGT GGCCAAAGCA 
    GTCTACGACA ACAGGCTATT AGAGTGTTAC TTTACACGCA GCTTCTACAA GCACATCCTG 
    GGCAAGAGCG TTAGGTATAC AGACATGGAA AGCGAAGACT ATCCCTTTTT CCAAGGTCTG 
    GTTTATTTGT TGGAGAACGA TGTGTCGACT CTGGGCTACG AGCTGACATT CAGCACAGAG 
    GTCCAAGAGT TTGGCGTGTG TGAAGTGAGA GATCTGAAGG CCAACGGTGC CAACATTCTT 
    GTCACAGAAG AGAATAAGAA GGAATATGTG CATCTGGTCT GCCAGATGAA GATGACAGGT 
    GCAATCCGTA AACAGCTGGC TGCCTTCCTG GAGGGCTTTT ATGAGATCAT CCCCAAGAGA 
    CTGATCTCCA TCTTCACTGA GCAAGAGCTG GAGCTGCTCA TCTCCGGCCT GCCCACCATT 
    GACATCGACG ACCTCAAGGC CAATACTGAA TACCATAAAT ATCAGTCCAG TTCTATTCAG 
    ATCCAGTGGT TCTGGCGTGC TTTACGATCC TTCGACCAGG CTGACAGGGC CAAATTTCTG 
    CAGTTTGTTA CGGGCACTTC CAAGGTGCCA CTGCAGGGCT TTGCTGCACT GGAGGGAATG 
    AACGGCATTC AGAAGTTCCA GATCCACCGA GATGACCGCT CCACTGACCG CCTGCCCTCT 
    GCGCACACTT GCTTTAACCA GTTGGACCTC CCAGCGTACG AGAGCTACGA GAAGTTAAGA 
    CACATGCTGC TGCTGGCCAT TCAGGAATGT TCTGAAGGCT TCGGACTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_009296963.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295238.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID ODa43094
    Clone ID Related Accession (Same CDS sequence) XM_009296963.2 , XM_009296963.3 , XM_021473711.1 , NM_001365655.1
    Accession Version XM_009296963.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296963.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA 
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC 
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC 
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT 
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG 
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT 
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC 
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC 
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC 
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT 
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG 
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA 
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG 
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA 
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC 
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT 
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA 
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT 
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC 
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG 
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG 
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG 
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT 
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG 
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT 
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT 
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC 
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC 
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC 
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295238.1
    CDS149..13684
    Translation

    Target ORF information:

    RefSeq Version XM_009296963.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009296963.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA 
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC 
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC 
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT 
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG 
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT 
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC 
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC 
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC 
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT 
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG 
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA 
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG 
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA 
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC 
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT 
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA 
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT 
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC 
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG 
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG 
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG 
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT 
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG 
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT 
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT 
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC 
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC 
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC 
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_009296963.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295238.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID ODa43094
    Clone ID Related Accession (Same CDS sequence) XM_009296963.2 , XM_009296963.3 , XM_021473711.1 , NM_001365655.1
    Accession Version XM_009296963.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296963.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA 
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC 
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC 
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT 
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG 
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT 
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC 
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC 
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC 
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT 
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG 
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA 
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG 
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA 
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC 
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT 
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA 
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT 
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC 
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG 
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG 
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG 
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT 
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG 
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT 
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT 
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC 
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC 
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC 
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295238.1
    CDS142..13677
    Translation

    Target ORF information:

    RefSeq Version XM_009296963.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009296963.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA 
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC 
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC 
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT 
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG 
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT 
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC 
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC 
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC 
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT 
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG 
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA 
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG 
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA 
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC 
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT 
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA 
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT 
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC 
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG 
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG 
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG 
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT 
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG 
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT 
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT 
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC 
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC 
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC 
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    huwe1 ( XM_021473711.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329386.1 Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID ODa43094
    Clone ID Related Accession (Same CDS sequence) XM_009296963.2 , XM_009296963.3 , XM_021473711.1 , NM_001365655.1
    Accession Version XM_021473711.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395155.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA 
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC 
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC 
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT 
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG 
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT 
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC 
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC 
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC 
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT 
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG 
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA 
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG 
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA 
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC 
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT 
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA 
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT 
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC 
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG 
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG 
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG 
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT 
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG 
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT 
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT 
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC 
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC 
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC 
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329386.1
    CDS142..13677
    Translation

    Target ORF information:

    RefSeq Version XM_021473711.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, UBA and WWE domain containing 1 (huwe1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021473711.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    13321
    13381
    13441
    13501
    ATGAAGGTTG ACCGAAGCAA ATTGAAGAAA ACCCCCACTG AAGCGCCTGC TGACTGCAGG 
    ACACTCATAG AGAAGCTAAA AGGATGCAGC GACGAACAGC TCTTGCTGGA ACTGCAGCAA 
    ATCAAGACCT GGAACATTGG CAAGTGCGAG TTGTATCATT GGGTGGACTT GCTGGACCGC 
    TTTGATGGCA TCCTCTGTGA TGCCGGGCAG ACAGTGGAGA ACATGTCTTG GTTGTTGGTG 
    TGCGACCGGC CAGAGAATGG GCAGTTAAAA GCCTTATTGC TGGCGGTCCT CAACTTCACC 
    GCGTTATTAA TCGAATACAG TTTTTCAAGA CACCTGTACA GCTCTATAGA GCACTTGACC 
    ACCCTTCTAG CATCATGTGA CATGCAGGTG GTTCTGTCCG TGCTGAATCT CCTGTATGTG 
    TTTAGCAAAC GTTCCAACTA CATCACCAGA CTAGGCTCAG ATAAAAGAAG CCCACTGCTT 
    GCCCGCCTGC AACATCTGGC TGAGAGCTGG GGAGGGAAAG AGAATGGTTT TGGTCTTGCA 
    GAGTGTTGCA GAGATCTGCC GATGTCGAAA TATCCACCGA GTGCCACCAC ATTGCACTTT 
    GAGTTCTATG CAGAGCCCAG CTCTGAGGTC AAAGTGGAAA AGAAGTCAAG CAGTAATACG 
    CTACACTACA TTCATATCGA ACAACTTGAT AAGATTTCTG AAAGCCCCTC TGAGATCATG 
    GAGTCTCTAA CGGCTATGTA CAACATACCT AAAGACAAGC AAATATTGCT TTTTACACAC 
    ATTCGCCTGG CTCACGGCTT CTCCAACCAC AAGAGGAGAC TGCAGGCTGT TCAAGCCAGG 
    CTGCATGCCA TCTCCATACT CGTGTACTCC AACGCCCTCC AGGAGTCAGC CAATAGCATA 
    CTCTATAATG GGCTTATAGA GGAGCTAGTG GATGTACTTC AGATCACAGA CAAACAGCTG 
    GTGGACATTA AGGCTGCTTC TTTGCGGACG TTGACATCAA TAGTCCACCT GGAACGCACC 
    CCCAAGCTCT CCAACATCAT CGACTGCACA GGAACAGCCT CCTATCATGG ATTTCTGCCT 
    GTGCTTGTTC GCAACTGCAT ACAGGCAATG ATCGACCCAC TGATGGAGCC GTACCCCCAC 
    CAGTTTGCCA CAGCCCTCTT CTCCTTCCTC TACCACTTGG CTAGCTATGA CGCAGGTGGA 
    GAGGCCTTGG TGTCTTGTGG TATGATGGAG GCATTGCTAA AGGTGATCAA GTTCCTAGGG 
    GACGAGCAGG ACCAGATTAC CTTTGTGACG CGGGCAGTGA GAGTTGTGGA TCTCATCACC 
    AATCTGGACA TGGCCGCTTT TCAGTCCCAC GGCGGCCTCT CAATCTTTAT CTGCAGACTG 
    GAGCATGAGG TTGACCTCTC CAGAAAAGAG TGCCCTTTTG TAATCAAACC AAAGATCCAG 
    AGGCCCAGCA CAGCCACTGA GACAGAGGAC ATGGATACAG ATATGGACAT GTCCGAGGTT 
    GCCATGGAGA GCAGTCCTGG ACCATCCACT TCTTCTGGCT CCAGAGCAGA GACCGATTCG 
    CGAGCACAAA GCAATGCATC CAGCACCCCA AGAGCAGGAG TGCAGTGCAT CCCTCAAAGG 
    GCCGCTCTGT TGAAATCCAT GTTAAACTTT TTGAAAAAAG CCATTCAGGA CCCTGCTTTC 
    TCTGATGGCA TTCGCCATGT AATGGATGGG TCCCTTCCTA CCTCTCTGAA ACACATTATC 
    AGTAATGCAG AGTATTATGG ACCATCACTG TTTCTCCTGG CGACGGAGGT GGTGACCGTG 
    TTTGTGTTCC AGGAGCCCTC TTTACTATCA TCTCTGCAGG ACAATGGTCT CACCGATGTC 
    ATGCTACATG CACTGCTCAT CAAAGACGTC CCTGCCACCA GAGAGGTTCT GGGTTCTCTG 
    CCTAATGTCT TCAGTGCCCT GTGCTTGAAT GCTCGTGGTC TGCACTCATT TGTGCAGTGC 
    CAGCCCTTTG AGCGGCTGTT CAAGGTGCTG CTCTCACCAG ACTACCTGCC TGCCATGCGG 
    CGCAGACGTA GCTCTGACCC TCTAGGTGAC ACTGCTTCTA ACTTGGGCAG CGCTGTAGAC 
    GAGCTTATGC GGCACCAGCC CACACTTAAA ACGGATGCAA CAACAGCCAT TATCAAGTTG 
    CTAGAGGAGA TCTGCAACTT GGGCAGATCA CCGGAGTACA TCTGCCAGAA ACCCTCCATA 
    CAAAAAGCAG ATGGGACCGT TACAGTGCCA CCAGCCCGAT CAAACCATGC TGCAGAGGAG 
    GCGTCCAGTG AGGATGAGGA GGAAGAGGAG GCTCTGCATA CCTTTAGCCA ACAGCAGGGG 
    GAGCCAGAGA GCAACAGGCA AGTGGTGGGA ACAGAGGAAC GAATCCCCAT TCCTCTTATG 
    GACTACATTC TGAATGTGAT GAAATTTGTG GAGTCCATTC TTAGTAACAA CACAACAGAT 
    GACCACTGTC AGGAATTTGT CAACCAGAAA GGACTCCTAC CACTGGTGTC CATCCTTGGG 
    CTGCCAAACC TGCCTATTGA CTTCCCCACC TCTGCTGCCT GCCAAGCTGT GGCAGGAGTC 
    TGCAAATCTA TACTAACTTT ATCTCATGAG CCAAAAGTCC TCCAGGAGGG TCTGTGTCAG 
    TTGGACAGCA TACTCTCTGC TCTGGAGCCA CTTCACCGGC CCATTGAGGT TCCTGGAGGC 
    TCTGTTCTGC TCAGAGAGCT GGCTAACGCA GGCCATGTCA CAGACGCCAC ACTGTCTGCA 
    CGCGCCACCC CACTGCTGCA TGCCCTCACA GCGGCACATG CGTACATCCT CATGTTCGTT 
    CATACCTGCA GAGTCGGACA GAGCGAGATT CGTGCTATCT CTGTGAATCA ATGGGGATCT 
    CAACTGGGTC TTAGTGTTTT GAACAAACTG AGTCAGCTGT ATTGCTCTCT GGTGTGGGAG 
    AGCACTGTGC TACTGTCCCT CTGTACACCA AACAGTTTGC CTCCTGGCTG TGAGTTTGGT 
    CAGGCTGACA TGCAGAAACT TGTGCCTAAA GAGGATAAAC CTTCTAGTAG CACTGCAGCT 
    ACAGGACGGA GAACTGCTGA GACCGAGTCC TTGTCTGCGG GGGTGGATCC CTCTGCTCAG 
    GGTTTGCTGG AAGGAATGGG ACTGGATGGA GACTCTCTGG CTTCAATGGA GACGGACGAG 
    CCCACAGCCA CTGACCCCAA GACCAAGTCC AAACTGACCC CTGCCATGGC AACACGCATC 
    AAACAGATAA AGCCTCTGCT CTCAGCCTCG TCTCGATTGG GGAGGGCTCT GGCTGAGCTG 
    TTTGGACTGC TGGTGAAGCT TTGTGTGGGC TCACCTGTTC GTCAACGGCG CTCTCACCAT 
    ACCACCAGCA CAGGAACTGC CCCGACACCG GCAGCACGTG CCACCGCTTC TTCTCTTACT 
    AAACTCCTTA CCAAGGGTTT GAGCTGGCAG CCTCCTCCCT ACACACCAAC ACCACGCTTC 
    AGGCTGACTT TCTTTATCTG CTCTGTGGGT TTCACTTCAC CCATGCTGTT TGATGAGAGG 
    AAGTACCCAT ACCATCTGAT GCTGCAGAAG TTCTTCTGTT CTGGTGGACA TGATGCGTTG 
    TTTGAGACAT TTAACTGGGC CTTGTCCATG GGAGGTAAAG TGCCCGTGTC TGAAGGCTTG 
    GAACACTCAG AGCTGCCTGA TGGAACTGGA GAGTTCCTAG ACGCCTGGTT GATGCTAGTA 
    GAGAAAATGG TGAACCCCAG CACTGTTCTG GACTCTCCAC ATTCCCTACC TGCTAAAGTG 
    CCTGGAGCAA CATTCACTGC CCCGCAGTTC AGCGCCTTGC GCTTTCTTAT TGTCACTCAG 
    AAGGCCGCAT TCAGCTGTAT CCGTAACCTG TGGAACCGAA AACCCCTGAA AGTCTATGGT 
    GGACGCATGG CTGAGTCCAT GCTGGCCATT CTGTGCCACA TCCTCCGAGG AGAACCGATC 
    ATCCAGGAGC GACTCTCCAA AGAGAGGGAG GGCACTGCTC GGCCTGAAGA GGAAAGCAGC 
    TCCGCAAGCA CTGCGCCAGC CAGCGGCCCT TCAGGAACAG CCGGAGTAGT TGGAGAAGCA 
    GTGGCTGCAG GTGCCAATGG AAACACTGCT GGAGGCTCTA CAGAGGAGGC CAGCAACTCT 
    ACTACAGCAC GCAGAGAGCC ACAGGTCAAC CAAGCTCAGC TCACACAGCT AATGGACATG 
    GGCTTCTCCA GAGAGCACGC AATGGAGGCT TTGCTGAACA CCAGCACTAT GGAACAGGCC 
    ACTGAGTACC TGCTCACACA CCCTCCACCA CTGCTCAGCG CGGCTGTCAG AGAGTTCACC 
    ATGTCTGAAG AAGACCAGAT GATGCGGGCC ATTGCCATGT CTCTAGGTCA AGAAGTTAGC 
    ATGGAGCAGC GCTCAGACTC ACCTGAGGAA GCAGCTCGCA GGCGTGAGGA GGAGGAGAGG 
    AGAGCGAGGG AGCGGGTAGA GGAGGAAGAA GCTCGTTGTC TGGAGCGCTT TCTGGAAGCA 
    GAGCCTCTTG ACACCACTGA GCTTCATGCC TTCACAGACT CCATGCTGCC TGGCTGCTTC 
    CACCTGCTCG ATGAGCTGCC GGACACAGTC TACCGCCTCT GTGATCTGCT CATGACTGCT 
    ATTAAAAGAA ACGGCCCTGA ATACAGAGAC CTCATCCTTA GACAAGTGGT CAATCAGGTG 
    TGGGAGGCTG CTGACGTGTT GATAAAGGCT GCAGTGCCGC TAACCACCAG CGACACCAAG 
    ACAGTCTCTG AGTGGACCAG ACAGATGGCG ACCCTCCCTC AGGCCTCCAA CCTGGCCACC 
    CGCATCCTGC TGCTCACGCT GCTCTTTGAG GAGCTGAAAC TCTCCTGCGC TCGGGTGGTG 
    GAGAGCAGTG GTGTCTTGAC TGTGCTGATT AAGCTGTTGG AGGTGGTGCA GCCCTGTCTG 
    CAAGCTGCCA AAGAGCAGAA GGACATCCAA ACGCCCAAAT GGATCACCCC TGTGCTCCTA 
    CTTATTGACT TTTATGAGAA AATGGCTGTT TCATCCAAAC GAAGAGCTCA GATGAACAAG 
    TACCTCCAGC CCAATGGGAA TAACTGGCGC TGGTTTGATG ACCGTTCAGG CCGCTGGTGC 
    AGCTACAGTG CAAGTAACAA CAGCACTATT GACTCTGCAT GGAGGGCAGG AGAGACCAGT 
    GTCCGATTTA CAGCTGGCCG CAGAAGATAC ACAGTTCAGT TCAACACCAT GGTGCAGGTA 
    AATGAGGAGA CTGGCAACAG ACGACCAGTT ATGTTGACGG TACAGCGGGT GCCACGCATT 
    CCCAAGGCAA CAAAATCCGG AAGTGTTACT GACACGGAGA GAGAGGAGAG TGAACAGAGA 
    GCAAAGACTG AGGAAACACA GACTGACACA GATTCCAGCT CTGTGGCTGT AGAGATGGCG 
    CCCCCTAAAG AAGAATCTGA GCTGAAGGAT TCAACCCCAG CAGCTCCGTC AGTTCTCCAG 
    GACCCCTCTG GTTCTAGTGG TATCGTTGTG CAGGGGCTGA CAGAAGACAT GATCACCATT 
    TTAATCCGTG CCTGTGTCAG CATGATCAGT GTTCCTGTGG ACCCAGACAC TTTGCACGCC 
    ACCTTGCGGC TCTGTCTGCG CCTCACACGC ACACACCATT ACGCCATGAT GTTTGCAGAG 
    CTCAAGAGCA CCCGCATGAT TCTGAATCTC ACCCAGAGCT CAGGCTTTAA TGGCTTCACC 
    CCATTGGTCA CCCTGCTCTT CCGCCACATC ATAGAGGACC CGGCCACACT GCGGCACACT 
    ATGGAGAAGG TGGTACGTTC TGCAGTCACC AGTGGTGCAG GAAGCACTAC TTCAGGTGTG 
    GTGTCAGGTA GTCTGGGCTC CAGGGAAATT AACTACATCC TGCGAGTCCT TGGCCCAGCG 
    GCCTGTCGCA ATCCAGAGTG CTTTACGGAA ACTGCCAAGA GTTGCATCCG AATTGCTCTC 
    CCGGCTCCAA GAGGTGCTGG CACTGCCTCA GATGACGAGT TTGAGAACTT CAGAATTAAA 
    GGTCCAAATG CTGTGCAACT CGTGAAGACC ACCCCCCTAA AGCTCTCCCC GTTGCCCTCC 
    ATACCAGAGA CTATAAAAGA GGTCATCTAT GACATGCTGA ATGCCCTGGC AGCATATCAC 
    GCACCAGAAG AGGCAGAGAG AGGAGAGGAG CGGGCAAACG CTGTACCAGG GAATCAAGAC 
    CTGTGTCAGA TCCTGCAGGA TGTCGGTGAT GATGTCTACC AGCAGTACAG ACTCACCAGA 
    CAAGGCAGTG ATTTTGACTC GCAATCTGCC TTCCCCATCA ATACTCAGGT GTTTGCTGCA 
    GATGGTGCTG TAGCTGAATC TTCTCAGTCT GGAACTCCAC AGGGAGAAGC CTCTACACCC 
    GAGGAAATGC GTGAAGAGAA AAAGGAGCAG GAAGGAGACA AGGGAGCTTC TTCGGAGGAG 
    AGCCGAGCTG CTAAGGCTAA AGCTAGCAAA CCTCTGATGC CCACCTCTAC CATCTTGAGG 
    CTGTTGGCCG AGCTGGTTCG CTCTTATGTT GGCATTGCCA CTCTTATCAC CTCTTACAAC 
    TACACAGCTG GCCAGTCGGA GCTCATTAAG GAAGATTGCA GTGTGTTGGC ATTTGTTTTG 
    GACCACTTGC TCCCACACAC CCAGAACTCT GAAGATAAGG ACACCCCTGC CCTTGCACGC 
    CTTTTCTTGG CCAGCCTTGC TGCAGCTGGC ACGGGCACCG ATGCCCAGGC TGCACTGGTC 
    AACGAGGTCA AAGCTGCTTT GAGTCGAGCA TTGGGCATGG CAGAGGGCAC AGAGAAACAC 
    GCCCGTTTGC AAGCAGTGAT GTGCATCATC AGCACCATTA TGGAGTCGTG TCCTTCCACC 
    TCCAGCTTCT ACAGCACAGC CACAGCTAAG ACCCAGCACA ACGGCATGAA CAATATCATC 
    CGGCTCTTCT TGAAGAAAGG CCTTGTCAAT GACCTGGCCC GTGTGCCTCA CAGCCTTGAT 
    CTATCCAGTC CCAATATGGC AAACACAGTA AATGCTGCTT TGAAGCCTCT GGAAACATTG 
    TCGCGAATTG TGAACCAGCC CAGCAGCCTG TTTGGTGGTA AAGGGGGATC CAGCAAGAAC 
    AAGACTGAAC ATGACACTGT TGGCACAGTC AGAGACAGTA ACAGCAACAC TCAGGATCAA 
    GGAGAAGTGG GTGAGGCAGA GCCGGTTGAG AATCGTGACC GAGGTCAAGC CACAGACTCT 
    GACCTGATGG ATGGAGAAGC CGAGGGAGAC ACTGTGGTCA TTGCTGGACA GCCTGAGGTG 
    CTGAGCACTC AGGCAATGCA GGTGGAGAAC GAGTTGGTGG ATTTGATCGA TGAACTTTTA 
    GAGAGAGATG CCCAAACTGT TAATAGTACC ATCATCGTAG GTCGTTCTGG AGAGGATGAG 
    TCTCAAGAGG ATGTGCTGAT GGATGAAGCT CCTTCCAATA TGAGTCAGGC CTCCACTCTC 
    CAAGCCAACA GAGAAGACTC CATGAACATC CTCGAGCCAG AAGATGAGGA GCACACGCAG 
    GAGGAAGACT CCAGTGGTAG CAACGAGGAT GAGGACAGTC AGGATGAAGA GGAAGAGGAG 
    GAGGAAGAAG AGGAGGAAGA TCAGGATGAT GAAGAGGGAG ATGAGGATGA TGACGATGAA 
    GGCTCAGAGA TGGAACTGGA TGAAGATTTT CCAGATATCA ATGCTGCCCC TCATATTCGT 
    TTTGAGCGAT TCGACCGAGA TGATGACCTC ATCATTGAGT TTGATAATAT GTTCTCCACT 
    TCAGCTGACA TCCCTCCCTC ACCAGGAAAT ATCCCCAGCT CCCACCCTCT GATGGTGCGT 
    CACGCTGACC ATGGCTCGCT GACTCTTGGG GGATCGGGCA CCAGTAGCAG ATTGGCTCAA 
    GGAATGGGAC GTAGTCAACG TTCTCTCCGC CAGCTCACGG CCAACACAGG CCACACGATC 
    CATGTACATT ATCCTGGCAA CCGTCAGCCT AACCCTCCAC TCATTCTGCA GAGATTATTG 
    GGCCCCAGCG CTGCAGCTGA TATCTTGCAG CTGTCCAGCT CTCTTCCTCT GCAGTCTCGA 
    GGTCGTGCCC GTTTAATGGT GGGTAATGAA GATGTGCACA TTATTGCCCG TTCAGACGAT 
    GAGCTGCTGG ATGACTTCTT CCATGAGCAG AGCAGCACCG GAGGACAAGC TGGCACCCTT 
    TCAAGTATCC CGACTGCCTT AACCAGATGG ACAGATGAGT GTAAAGTGCT GGATGCTGAG 
    AGCATGCATG ACTGTGTGGC AGTGGTGAAG GTGCCCATAC TGCAGCACCT GGAGAGTTTG 
    CGTGATGAGG AGCTGGAGGA ACGCAGGGAG AAAAGGAGGA GGCAGTTGGC AGAGGAAGAG 
    GAGACTGCAA AGCAGAATGA GAGTTCTGCA GCAGGGGAGG AGACCAGGGA ACAGAGTCTG 
    CAGGGCTCTG GGATTGGAAC AGTCAATGGA GCTGCAGAGA ATGCTGCAGA AGGAGAACAG 
    GCCCAGAGTG GGGTGTCCTC CTGTCTGGAC CCTCCTCGCA CCTCTGAGGG TTTCCTCACA 
    GCGCCCCCAT CAGGAGAAGT CACTCCAACC ACCCCTGCCC CCCATGAACA AGCCCTCGTG 
    TCTCTGGAAA CAGCCATCAG TCAGCAGGTG CACCAGCCAA TCACAGAGCT GCTGCTGGCC 
    GACTCCAATC CTGACTCTCA CGGAGCCTTG GCAGGGGCAG GGCTTCCCCA GCTCTCAGCA 
    CCTGATAGGC CAAATTGTGA GGCAGAAGCA TCTCAGATGG AGATGTCACC TGCACCCACT 
    ATTGGTGAGA GAGGAGGAGG AGCATTGGAT GCAGTCAGGG AAGCTTCACT CAGTCCAGAT 
    ATAGCTGAAA GTTCAGAGCC TGTGCCAGTG GGTGTGTCTC AACTCGAGGG TTCGCCCATG 
    GATACGAGCA GTCCCGCTTC AGGAACGCAG GAAGAGCCGG CTGCCAGCTC AGTTCCATCC 
    AATCACATGT CTCAGGAGCT ATCAGGAAGT GGTGACAGCG GCCTCACAGA CAGGCAGACA 
    GATGCAGAAA CTGGATCAAC TTCTGTTTCA TCTCCTGGCG AGACCATGCC TCGGAGTGAC 
    AGTGCCGATA GCCAGTCGCA GGCCATCCAG GAGGAGCCCC TCCCTTCCAC CAGCAATGAA 
    GAGGAGGACC CTCTGGCAGG CATCAGTCTA CCTGAAGGCG TGGATCCGTC TTTCTTGGCA 
    GCACTGCCTG AAGACATTCG TCGGGAGGTG CTGCAGAACC AACTGGGAAT CAGACCTCCT 
    TCTCGACCTC CTGTCGCCTC CACTCTGACA ACCACTGCTA GTGCTGTGCT AGGAGGAGGG 
    CCAGGGGTGA CCGAAGTCAG CCCTGAATTC TTAGCGGCTC TCCCACCTGC AATTCAGGAA 
    GAGGTCCTGG CCCAGCAGCG GGCAGAGCAG CAGCGTCGGG AGTTATCCCA GCAACCCACA 
    CAGGGTGATC AACCTCTGGA CCCAGTCACA TTCATCCAGA CCCTGCCCTC TGAGCTGCGG 
    CGCTCCGTTC TGGAAGACAT GGAGGACAGT GTACTAGCGG TCATGCCTCC AGATATTGCA 
    GCAGAGGCTG CAGCACTGCG AAGGGAACAA GAGGCCAGAC AGAGACAACT GATGCACGAG 
    CGCTTGTTCG GTCACAGCTC CTCTTCAGCT CTGTCTGCCA TCTTACGCTC TCCCGCCTTC 
    ACCAGCCGCC TCGGGGGCAA CCGTGGGGTA CAGTACACCC GGCTGGCTGT GCAAAGAGGA 
    GGGACGTTTC AAATGGGAGG GAGTGCCAAT CATAGCAGAC CCTCTAGTTC AAACGTTGAC 
    TCTCTCCTGC GCCTGCGGGG GCGTCTCTTG TTGGACCACG AGGCCTTGTC CTGCCTTCTT 
    GTGCTGCTTT TTGTGGACGA GCCCAAACTT AACACCAGCC GCCTCCACCG AGTGCTGCGT 
    AACTTGTGCT ATCACTCTCA GACGCGTGGC TGGGTCATCC GCAGTCTCCT GTCCATCCTG 
    CAGCGCAGCA GTGAGAGCGA GCTGTGCCTG GAGACTGCAC GCTTGGAGGA GTCCCGTGGC 
    AAACGCTCAC TAGGCCAGGG CTCATCTAGT GGAGGAAAGG GTTGTTCTTC ATCCTCCCAG 
    TCGGCTATAG CCTCTTCGTC ATCGTCCTCC TCGTCCTCTT CTTCTCTGGA GCTGCTGAAC 
    AGGGTGGAGT CACGGAGTTC CAGTCAGCTC TCCTGGCTGT CTGTATCAAT GGACGCAGCT 
    CTTGGATGCA GAACCAACAT CTTTCAGATC CAGCGAGTAT CAGGCAGGAA ACACGCAGAG 
    AGGCATTCAG CAGGAGGGAG TGTGAGCGCA GGAGGACTCA CAGGTGGAGT GTCAGGAACA 
    GGAATGACGT GTACAGGGGG AGGTGGGTCA ACTGTTCACA TCCATCCACA GGCTGCGCCG 
    GTGGTCTGTC GACATGTACT GGACACCCTC ATCCAATTGG CCAAGGTATT TCCAAGTCAC 
    TTCACTCAAC AACGCTGTAA AGATCCCCTA TCCTCCTCCT CCTCTGAGTT GGACACTCGT 
    CTTTGTGCCA GCTCAACACC GACTATGAGT GCCAGTTGTC GGTCAGGTGG CACCAGTCAG 
    AGTAACTCCT CTGCTACCCC AAGTAGTCTC ACTTCTATCC ACAATTCCCT GAGCGCCTGC 
    TCTGGTACGC CACAGTCCCT AGGCATCTCC ACCGACTTTT GGGATCTCCT GGTCAAGCTG 
    GACAACATGA ATGTGAGCCG AAAGGGGAAG GCCTCCATGA AGACTGTCCC TCTAGGGGGT 
    GTCGGCGAGA CAGAGGGTGC GCTGTTCAGC CTTGAGGCGT CTCCGCTGGG CCAGCTGATG 
    AATATGCTGT CCCACCCTGT GATCCGCCGT AGTTCCCTCC TCACGGAGAA ACTGCTACGT 
    CTGCTGTCTC TGATCTCCAT CGCTCTGCCT GACAACAAGG CCACGGAGGT TCCAGCCAGC 
    CATCCAGTGC CTCAGACCCC AACTGCCACG CCATCTGCAC CTGCTCCTCA AACCACAGCA 
    GGGTCAGGGA CCATCTCAGC AGTGGCAGCT ACAGGCACTA CGCAGGGTTC AGTCGGTTCA 
    GTGGGTACAA CGCCATCTCA ACCAGCCGCT ACAATATCTG TGGTTGCAGG CAGCACCACC 
    GCCTCTAGCA TTATTGCTGG AAAACTGAAG CCAGCAGTAA GCATCACGGA CATTGAATCT 
    AAACTGGCCT CTACTGGACT TACGGAGAAG CAGCTGCAGC TCTCTGTGGA GGTTCTGACA 
    TCTCACTCGT GTTCAGAGGA AGGTCTGGAG GACGCAGCCA ACATCTTGCT GCAGCTGTCC 
    AGAGGGGACG CACCGACCCG AGACACTGTG CTTAGACTTC TGCTCAGCGG AGCACGTCAC 
    CTTGGATACA CTCTCTGCAA ACAAATCGGC ACTTTATTAG CTGAGTTGAG GGAGTATAAT 
    CTAGAGCAGC AGAGAAGAGC ACGGGCTGAT GCTCAGTCTC CTGAAGCTTC TCATGATGAC 
    CCATCCATCA CCACACGCCT CAAGGGCAAG ATGACCAGCA GGTTTGACGG GTCAGAGAGT 
    GTGGTGATTG TTGCTGCTCA GAAGCGCACG TTAGGTGGCC GGGAGCTTCA GTTGCCCTGC 
    ATGAGCTCGC TCACTTCCAA GACCTCAACG CAGAAGTTTT TCTTGCGGGT ACTGCAGGTC 
    ATCATCCAGC TTAGAGAAGA CACGCGGCGC GCTAACAAGA AAGCCAAGCA GACAGGAGGA 
    AGACTAAGTT CAACCAGTCT GGGCTCGGCC AGTAGTATTC AGGCTGCGGT CCGTCAGCTG 
    GAGGCTGAAG CGGACGCCAT CATTCAGATG TCGGAGGTTC ACACTGTTGC CGAGCCACCA 
    GCAGCTCAGA GAGATGATTC GCCCATGGAT GTGGATCAGC CCTCTCCTTT GGAACAGGAC 
    CAGCCAACAT TAGGTGAGGA GAGCAGCAGT CAGCAGGAGC AGGAGGAGCG TTTGCCTGAC 
    CTGCCGCTGT TAAGTGAGCA GCTGCTTTTG GATGAGCTGT GGGACATGCT TGGAGAATGT 
    CTGAAAGAGC TTGAAGAGTC TCATGACCAA CATGCAGTTC TAGTGCTGCA GCCTGCAGTG 
    GAGGCCTTTT TCTTGGTGCA TGCAACAGAG CGGGAAAGCA AACCACCAGT TCGGGACACT 
    CGGGAGAGCC AGCTGTCACA CATCAAGGAT GAGCCTCCAC CCCTGTCACC CGCCCCTCTC 
    ACACCCGCTA CCCCCTCCTC TCTGGACCCC TTTTTTTCCA GAGAGCCTTC CTCTATGCAC 
    ATCTCTTCCA ACCTGCCTCC AGATACACAG AAGTTCCTCC GTTTCGCAGA GACCCACCGC 
    ACAGTGCTGA ATCAGATCCT GCGTCAATCC ACCACTCACC TGGCAGACGG GCCTTTCGCT 
    GTTTTGGTTG ACTACATTCG CATCTTGGAC TTTGACGTCA AGCGCAAGTA TTTCCGTCAG 
    GAGCTGGAGC GTCTGGATGA AGGCTTGAGG AAAGAGGATA TGGCCGTCCA TGTGAGGAGA 
    GATCACGTCT TTGAAGATTC TTACAGAGAG CTGCACCGCA AGAGCCCAGA AGACATGAAG 
    AACAGATTGT ATATAGTATT TGAAGGAGAG GAGGGTCAGG ATGCAGGCGG GCTGTTAAGA 
    GAGTGGTACA TGATCATCTC CAGAGAAATG TTCAACCCCA TGTATGCCCT GTTTCGCACC 
    TCCCCCGGCG ATCGGGTCAC CTATACCATC AACCCCTCCT CCCACTGCAA CCCCAACCAT 
    CTCAGCTACT TCAAGTTTGT GGGACGTGTG GTGGCCAAAG CAGTCTACGA CAACAGGCTA 
    TTAGAGTGTT ACTTTACACG CAGCTTCTAC AAGCACATCC TGGGCAAGAG CGTTAGGTAT 
    ACAGACATGG AAAGCGAAGA CTATCCCTTT TTCCAAGGTC TGGTTTATTT GTTGGAGAAC 
    GATGTGTCGA CTCTGGGCTA CGAGCTGACA TTCAGCACAG AGGTCCAAGA GTTTGGCGTG 
    TGTGAAGTGA GAGATCTGAA GGCCAACGGT GCCAACATTC TTGTCACAGA AGAGAATAAG 
    AAGGAATATG TGCATCTGGT CTGCCAGATG AAGATGACAG GTGCAATCCG TAAACAGCTG 
    GCTGCCTTCC TGGAGGGCTT TTATGAGATC ATCCCCAAGA GACTGATCTC CATCTTCACT 
    GAGCAAGAGC TGGAGCTGCT CATCTCCGGC CTGCCCACCA TTGACATCGA CGACCTCAAG 
    GCCAATACTG AATACCATAA ATATCAGTCC AGTTCTATTC AGATCCAGTG GTTCTGGCGT 
    GCTTTACGAT CCTTCGACCA GGCTGACAGG GCCAAATTTC TGCAGTTTGT TACGGGCACT 
    TCCAAGGTGC CACTGCAGGG CTTTGCTGCA CTGGAGGGAA TGAACGGCAT TCAGAAGTTC 
    CAGATCCACC GAGATGACCG CTCCACTGAC CGCCTGCCCT CTGCGCACAC TTGCTTTAAC 
    CAGTTGGACC TCCCAGCGTA CGAGAGCTAC GAGAAGTTAA GACACATGCT GCTGCTGGCC 
    ATTCAGGAAT GTTCTGAAGG CTTCGGACTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

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