HERC4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	HERC4
Entrez Gene ID	557513
Full Name	HECT and RLD domain containing E3 ubiquitin protein ligase 4
General protein information	Preferred Names HECT and RLD domain containing E3 ubiquitin protein ligase 4 Names probable E3 ubiquitin-protein ligase HERC4 hect domain and RLD 4
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005173035.4	XP_005173092.1	probable E3 ubiquitin-protein ligase HERC4
XM_005173035.3	XP_005173092.1	probable E3 ubiquitin-protein ligase HERC4

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006994	XP_308776.4
Homo sapiens (human)	HERC4	NP_071362.1
Canis lupus familiaris (dog)	HERC4	XP_005618991.1
Danio rerio (zebrafish)	herc4	XP_685685.5
Drosophila melanogaster (fruit fly)	CG9153	NP_001261249.1
Bos taurus (cattle)	HERC4	NP_001070362.2
Oryza sativa (rice)	Os02g0554100	NP_001047115.1
Pan troglodytes (chimpanzee)	HERC4	XP_001167753.1
Mus musculus (house mouse)	Herc4	NP_084390.1
Rattus norvegicus (Norway rat)	Herc4	NP_001012074.1
Gallus gallus (chicken)	HERC4	XP_004942077.1
Arabidopsis thaliana (thale cress)	UVR8	NP_201191.1
Xenopus tropicalis (tropical clawed frog)	herc4	NP_001122122.1
Oryza sativa (rice)	Os04g0435700	NP_001052849.1
Macaca mulatta (Rhesus monkey)	HERC4	XP_001087608.1

Gene Ontology

The following HERC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HERC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa19161	XM_005173035.4 Latest version!	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa19161	XM_005173035.3	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HERC4 ( XM_005173035.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005173092.1 Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), mRNA.

Price & Availability↑

CloneID	ODa19161
Clone ID Related Accession (Same CDS sequence)	XM_005173035.4 , XM_005173035.3
Accession Version	XM_005173035.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	probable E3 ubiquitin-protein ligase HERC4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173035.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATGTT GGGGAAACGC CTCCTTCGGG CAGCTGGGTT TAGGAGGTAT CGATGAGGAG 
GTGCTGCTGG AGCCCCGAAG ATGTGAGTTT TTCGAGGGTA AGCGCGTGCG GGACGTGGGC 
TGCGGGAGAC GCCACACTGT CTTCCTGCTA GAGGATGGGA CGGTTTACAC CTGCGGCTGC 
AATGACCTGG GGCAGCTGGG GCATGAGAAA GCCCGCAAGA AACCAGAGCA GGTGGTGTCT 
CTGGATGCGC AGAACATTGT GGCGGTGTCG TGTGGAGAAG CTCATACTCT TGCTCTGAAT 
GATAAAGGAC AAGTGTTTGC GTGGGGTTTG GGATCAGATG GACAGCTCGG ACTTTCCATT 
GTAGAAGACT GTCTGCGTGT GCCCAGAACT GTCAAGAGCT TGTCAGAGGT TCACATTACC 
CAGGTGGCCT GTGGATACTG GCATTCCCTT GCTTTAGCCC GAGGAGGCGA GATATTCTCC 
TGGGGCCAGA ATAAGTATGG TCAGCTGGGT CTGGGGCAGC AGGGAGCCTG TGTCTGCTCT 
CCACAGGTCA TCCAGTCTCT GCAGGGCATC CCGTTCGGGC AGATTTCCGC CGGTGGAGCT 
CACAGCTTTG GCCTCACGCT CTCTGGGGCC ATTTTCGGAT GGGGGCGGAA CAAGTTCGGC 
CAGCTGGGCC TCGGAGACAA TGATGATCGC CGTTTCCCAG TCTTGCTGAA GACTCTCAGG 
TCTCAACAGG TTGTTTACAT CTGCTGTGGA GAGGACCACA CGGCAGCGCT AACAAAGGAG 
GGCGGTGTAT TTACTTTTGG AGCTGGTGGA TATGGTCAGC TGGGACACAA CTCCACAAAC 
CATGAGGTCA ACCCCAGAAA GGTGTTTGAG CTCATGGGGA GCGTGGTTTC ACAGATTGCA 
TGTGGTCGGC AGCACACTCT GGCGTTCATC CCTTCATCAG GAAAGATAGA TTCGTTCGGT 
CTCGGGGGAA ACGGTCAGCT GGGAACGCGC TCCACCTGCA ACCGCATCAG TCCCACACCG 
GTGAAGGGCT GCTGGAGAGC CCACGCTGAC CCTGTTGCCA TGGATCATAG CGAGGCGGAG 
CGGAGTTACT GTGTGAAGAG GATTTATGCT GGAGGAGACC AGAGTTTCGC TCACTACGCT 
TTATCAGAGG ATGACGGTCC CCCACTGGAT GTGAGGATAA CCAGACCTGC TGGACAGATC 
TACACACTCA GCACTGAACT CATCCAGAAG TGGCTCAATC ACGGCCTTGG CAGGATACCA 
GCAGAAATCA CAGATGAGAT TGATCTTGTG TTTTCTTCTG CGAGTTGTCT CAATGGAAGC 
TTTCTGTCTT CAAGTAATCC AGATCACTAC AGCACGAGCA GTAAGTGCTC AGGTGTGGAC 
ATTAACACGG CTCGTCTGCT CTTCCACAAA CTCATCCAAA CCGATCACCC ACAAATCTCA 
CAAATCATCG CTGCTAGTTT GGAGAAGAAT CTCCTTCCCA AATTGAGCAG TTCTCCTCCA 
GACATCGAGG CTCTGAGACT GTACCTGACC CTGCCTGAGT GTCCACTTCT CTGCAACCCG 
AACAACTTCA CTACGTTAAC TATTCCCTTC GCCAAAGCCA TCGTCAGCCT GAAAGATGCT 
CCTATCAAAG TGCTCGGGAA CTGGTGGTCC CGGTTTGAGC CGGTGGTTTT CCAGAGGCTG 
GTGGAGCTCT ATAAGGAGGT GGTGGTGTAT CTGTTGCGCA TGCAGAAACT GGGCATTCCT 
CTTTCTGAAC AGAGGATCTT CACCTGCTTT TTAGACACTT CACTTAAACT GCTGGAGATC 
CTGCACTCGG TCAATGAGCG TGCCGGACAG ATCATCCAGT ATGATAAATT CTACATCCAT 
GAGCTAGACG AGCTGATCGA CATCAGGAAT GATTATGCGA CCTGGTTTCA GCAGCAGATG 
TTCTCTGTGG TCATGGACTC TCAGGTCACC CTCTGCAAAT ACCCGTTTGT GTTTGACGCT 
CAGGCGAAGA CGGCTCTGCT GCAGACTGAT GCTGTGATCC AGATGCAGAT GGCGGTGGAT 
CAGGCGCAGA GACAGAACTT CACCTCATTA TTTCTGTCCG GCGGAGGCGT GGAGTCAGTG 
AACCCCTGCC TCATCCTCAT CGTACGCAGA GAAAACGTGG TCGGAGACTC CATGGAGGTG 
CTGAGGAAGT CCAAAAACGT AGACTACAAG AAGCCACTCA AGGTGATCTT TGTTGGCGAG 
GAGGCTGTGG ACGCTGGTGG TGTGAGGAAA GAGTTTTTCC TGCTCATCAT GAAAGAGCTT 
CTGCACCCGA AATATGGCAT GTTTAGATTC CACGAGGAGT CCAGGCTTAT CTGGTTTGCT 
TGCAAGACGT TTGAAGATAT CGACCTGTTT CACCTGATCG GTGTTGTCTG TGGATTGGCC 
ATCTATAATC TCACTATAGT GGAGCTGAAT TTCCCGCTAG CGCTGTACAA GAAACTGCTG 
AAGAAAACAC CAACACTAGA GGACCTGAAG GAGCTCATGC CTGATGTGGG AAGGAGTCTT 
CAGCAGCTGC TGGATTATAC TGAAGATGAT TTGGAGGAAA CCTTCTGCCT GAATTTCACA 
ATCACAGAAG ATAATTTTGG AGCCACAGAG GTAGTGGAAC TGATCCCCAA CGGGACCGAC 
ATCAGCGTCA ACAAATCCAA CAGGCAGGAG TTTGTCAACG CTTATGTGGA TTATATATTC 
AACAAGTCTG TGGCTCCTCA GTTCAGTGCT TTCTATGCTG GATTTCACAA AGTGTGTGGA 
GGGAAAGTCC TGGAGCTTTT CCAGCCCAGT GAACTGCAGG CCATGGTCAT CGGAAACACC 
AACTATGACT GGAAGGAGCT GGAAAAGAGC ACCGAGTATA AGGGCGAGTA CTGGGCTGAT 
CATCCCACCA TCAAGATATT CTGGGAGGTT TTTCACGAGC TGCCTTTGGA GAAGAAGAAA 
CAGTTCCTGT TGTTCCTGAC AGGCAGTGAT CGTATCCCCA TTCTGGGCAT GAAGAGTCTG 
GCTCTGGTGA TTCAGCCGAC CGCAGGAGGA GAGCAGTATT TCCCAGTGGC ACACACATGC 
TTTAACCTGC TGGATCTGCC CAAATACACC AGCAAAAACA CAATGCAGGA GAAGCTGCTG 
CACGCCATTG AACACAACCA GGGCTTTAAC CTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173092.1
CDS	247..3405
Translation	MLCWGNASFGQLGLGGIDEEVLLEPRRCEFFEGKRVRDVGCGRRHTVFLLEDGTVYTCGCNDLGQLGHEKARKKPEQVVSLDAQNIVAVSCGEAHTLALNDKGQVFAWGLGSDGQLGLSIVEDCLRVPRTVKSLSEVHITQVACGYWHSLALARGGEIFSWGQNKYGQLGLGQQGACVCSPQVIQSLQGIPFGQISAGGAHSFGLTLSGAIFGWGRNKFGQLGLGDNDDRRFPVLLKTLRSQQVVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGSVVSQIACGRQHTLAFIPSSGKIDSFGLGGNGQLGTRSTCNRISPTPVKGCWRAHADPVAMDHSEAERSYCVKRIYAGGDQSFAHYALSEDDGPPLDVRITRPAGQIYTLSTELIQKWLNHGLGRIPAEITDEIDLVFSSASCLNGSFLSSSNPDHYSTSSKCSGVDINTARLLFHKLIQTDHPQISQIIAASLEKNLLPKLSSSPPDIEALRLYLTLPECPLLCNPNNFTTLTIPFAKAIVSLKDAPIKVLGNWWSRFEPVVFQRLVELYKEVVVYLLRMQKLGIPLSEQRIFTCFLDTSLKLLEILHSVNERAGQIIQYDKFYIHELDELIDIRNDYATWFQQQMFSVVMDSQVTLCKYPFVFDAQAKTALLQTDAVIQMQMAVDQAQRQNFTSLFLSGGGVESVNPCLILIVRRENVVGDSMEVLRKSKNVDYKKPLKVIFVGEEAVDAGGVRKEFFLLIMKELLHPKYGMFRFHEESRLIWFACKTFEDIDLFHLIGVVCGLAIYNLTIVELNFPLALYKKLLKKTPTLEDLKELMPDVGRSLQQLLDYTEDDLEETFCLNFTITEDNFGATEVVELIPNGTDISVNKSNRQEFVNAYVDYIFNKSVAPQFSAFYAGFHKVCGGKVLELFQPSELQAMVIGNTNYDWKELEKSTEYKGEYWADHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLALVIQPTAGGEQYFPVAHTCFNLLDLPKYTSKNTMQEKLLHAIEHNQGFNLV

Target ORF information:

RefSeq Version	XM_005173035.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173035.4

ORF Insert Sequence:

ATGTTATGTT GGGGAAACGC CTCCTTCGGG CAGCTGGGTT TAGGAGGTAT CGATGAGGAG 
GTGCTGCTGG AGCCCCGAAG ATGTGAGTTT TTCGAGGGTA AGCGCGTGCG GGACGTGGGC 
TGCGGGAGAC GCCACACTGT CTTCCTGCTA GAGGATGGGA CGGTTTACAC CTGCGGCTGC 
AATGACCTGG GGCAGCTGGG GCATGAGAAA GCCCGCAAGA AACCAGAGCA GGTGGTGTCT 
CTGGATGCGC AGAACATTGT GGCGGTGTCG TGTGGAGAAG CTCATACTCT TGCTCTGAAT 
GATAAAGGAC AAGTGTTTGC GTGGGGTTTG GGATCAGATG GACAGCTCGG ACTTTCCATT 
GTAGAAGACT GTCTGCGTGT GCCCAGAACT GTCAAGAGCT TGTCAGAGGT TCACATTACC 
CAGGTGGCCT GTGGATACTG GCATTCCCTT GCTTTAGCCC GAGGAGGCGA GATATTCTCC 
TGGGGCCAGA ATAAGTATGG TCAGCTGGGT CTGGGGCAGC AGGGAGCCTG TGTCTGCTCT 
CCACAGGTCA TCCAGTCTCT GCAGGGCATC CCGTTCGGGC AGATTTCCGC CGGTGGAGCT 
CACAGCTTTG GCCTCACGCT CTCTGGGGCC ATTTTCGGAT GGGGGCGGAA CAAGTTCGGC 
CAGCTGGGCC TCGGAGACAA TGATGATCGC CGTTTCCCAG TCTTGCTGAA GACTCTCAGG 
TCTCAACAGG TTGTTTACAT CTGCTGTGGA GAGGACCACA CGGCAGCGCT AACAAAGGAG 
GGCGGTGTAT TTACTTTTGG AGCTGGTGGA TATGGTCAGC TGGGACACAA CTCCACAAAC 
CATGAGGTCA ACCCCAGAAA GGTGTTTGAG CTCATGGGGA GCGTGGTTTC ACAGATTGCA 
TGTGGTCGGC AGCACACTCT GGCGTTCATC CCTTCATCAG GAAAGATAGA TTCGTTCGGT 
CTCGGGGGAA ACGGTCAGCT GGGAACGCGC TCCACCTGCA ACCGCATCAG TCCCACACCG 
GTGAAGGGCT GCTGGAGAGC CCACGCTGAC CCTGTTGCCA TGGATCATAG CGAGGCGGAG 
CGGAGTTACT GTGTGAAGAG GATTTATGCT GGAGGAGACC AGAGTTTCGC TCACTACGCT 
TTATCAGAGG ATGACGGTCC CCCACTGGAT GTGAGGATAA CCAGACCTGC TGGACAGATC 
TACACACTCA GCACTGAACT CATCCAGAAG TGGCTCAATC ACGGCCTTGG CAGGATACCA 
GCAGAAATCA CAGATGAGAT TGATCTTGTG TTTTCTTCTG CGAGTTGTCT CAATGGAAGC 
TTTCTGTCTT CAAGTAATCC AGATCACTAC AGCACGAGCA GTAAGTGCTC AGGTGTGGAC 
ATTAACACGG CTCGTCTGCT CTTCCACAAA CTCATCCAAA CCGATCACCC ACAAATCTCA 
CAAATCATCG CTGCTAGTTT GGAGAAGAAT CTCCTTCCCA AATTGAGCAG TTCTCCTCCA 
GACATCGAGG CTCTGAGACT GTACCTGACC CTGCCTGAGT GTCCACTTCT CTGCAACCCG 
AACAACTTCA CTACGTTAAC TATTCCCTTC GCCAAAGCCA TCGTCAGCCT GAAAGATGCT 
CCTATCAAAG TGCTCGGGAA CTGGTGGTCC CGGTTTGAGC CGGTGGTTTT CCAGAGGCTG 
GTGGAGCTCT ATAAGGAGGT GGTGGTGTAT CTGTTGCGCA TGCAGAAACT GGGCATTCCT 
CTTTCTGAAC AGAGGATCTT CACCTGCTTT TTAGACACTT CACTTAAACT GCTGGAGATC 
CTGCACTCGG TCAATGAGCG TGCCGGACAG ATCATCCAGT ATGATAAATT CTACATCCAT 
GAGCTAGACG AGCTGATCGA CATCAGGAAT GATTATGCGA CCTGGTTTCA GCAGCAGATG 
TTCTCTGTGG TCATGGACTC TCAGGTCACC CTCTGCAAAT ACCCGTTTGT GTTTGACGCT 
CAGGCGAAGA CGGCTCTGCT GCAGACTGAT GCTGTGATCC AGATGCAGAT GGCGGTGGAT 
CAGGCGCAGA GACAGAACTT CACCTCATTA TTTCTGTCCG GCGGAGGCGT GGAGTCAGTG 
AACCCCTGCC TCATCCTCAT CGTACGCAGA GAAAACGTGG TCGGAGACTC CATGGAGGTG 
CTGAGGAAGT CCAAAAACGT AGACTACAAG AAGCCACTCA AGGTGATCTT TGTTGGCGAG 
GAGGCTGTGG ACGCTGGTGG TGTGAGGAAA GAGTTTTTCC TGCTCATCAT GAAAGAGCTT 
CTGCACCCGA AATATGGCAT GTTTAGATTC CACGAGGAGT CCAGGCTTAT CTGGTTTGCT 
TGCAAGACGT TTGAAGATAT CGACCTGTTT CACCTGATCG GTGTTGTCTG TGGATTGGCC 
ATCTATAATC TCACTATAGT GGAGCTGAAT TTCCCGCTAG CGCTGTACAA GAAACTGCTG 
AAGAAAACAC CAACACTAGA GGACCTGAAG GAGCTCATGC CTGATGTGGG AAGGAGTCTT 
CAGCAGCTGC TGGATTATAC TGAAGATGAT TTGGAGGAAA CCTTCTGCCT GAATTTCACA 
ATCACAGAAG ATAATTTTGG AGCCACAGAG GTAGTGGAAC TGATCCCCAA CGGGACCGAC 
ATCAGCGTCA ACAAATCCAA CAGGCAGGAG TTTGTCAACG CTTATGTGGA TTATATATTC 
AACAAGTCTG TGGCTCCTCA GTTCAGTGCT TTCTATGCTG GATTTCACAA AGTGTGTGGA 
GGGAAAGTCC TGGAGCTTTT CCAGCCCAGT GAACTGCAGG CCATGGTCAT CGGAAACACC 
AACTATGACT GGAAGGAGCT GGAAAAGAGC ACCGAGTATA AGGGCGAGTA CTGGGCTGAT 
CATCCCACCA TCAAGATATT CTGGGAGGTT TTTCACGAGC TGCCTTTGGA GAAGAAGAAA 
CAGTTCCTGT TGTTCCTGAC AGGCAGTGAT CGTATCCCCA TTCTGGGCAT GAAGAGTCTG 
GCTCTGGTGA TTCAGCCGAC CGCAGGAGGA GAGCAGTATT TCCCAGTGGC ACACACATGC 
TTTAACCTGC TGGATCTGCC CAAATACACC AGCAAAAACA CAATGCAGGA GAAGCTGCTG 
CACGCCATTG AACACAACCA GGGCTTTAAC CTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_005173035.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005173092.1 Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), mRNA.

Price & Availability↑

CloneID	ODa19161
Clone ID Related Accession (Same CDS sequence)	XM_005173035.4 , XM_005173035.3
Accession Version	XM_005173035.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	probable E3 ubiquitin-protein ligase HERC4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173035.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATGTT GGGGAAACGC CTCCTTCGGG CAGCTGGGTT TAGGAGGTAT CGATGAGGAG 
GTGCTGCTGG AGCCCCGAAG ATGTGAGTTT TTCGAGGGTA AGCGCGTGCG GGACGTGGGC 
TGCGGGAGAC GCCACACTGT CTTCCTGCTA GAGGATGGGA CGGTTTACAC CTGCGGCTGC 
AATGACCTGG GGCAGCTGGG GCATGAGAAA GCCCGCAAGA AACCAGAGCA GGTGGTGTCT 
CTGGATGCGC AGAACATTGT GGCGGTGTCG TGTGGAGAAG CTCATACTCT TGCTCTGAAT 
GATAAAGGAC AAGTGTTTGC GTGGGGTTTG GGATCAGATG GACAGCTCGG ACTTTCCATT 
GTAGAAGACT GTCTGCGTGT GCCCAGAACT GTCAAGAGCT TGTCAGAGGT TCACATTACC 
CAGGTGGCCT GTGGATACTG GCATTCCCTT GCTTTAGCCC GAGGAGGCGA GATATTCTCC 
TGGGGCCAGA ATAAGTATGG TCAGCTGGGT CTGGGGCAGC AGGGAGCCTG TGTCTGCTCT 
CCACAGGTCA TCCAGTCTCT GCAGGGCATC CCGTTCGGGC AGATTTCCGC CGGTGGAGCT 
CACAGCTTTG GCCTCACGCT CTCTGGGGCC ATTTTCGGAT GGGGGCGGAA CAAGTTCGGC 
CAGCTGGGCC TCGGAGACAA TGATGATCGC CGTTTCCCAG TCTTGCTGAA GACTCTCAGG 
TCTCAACAGG TTGTTTACAT CTGCTGTGGA GAGGACCACA CGGCAGCGCT AACAAAGGAG 
GGCGGTGTAT TTACTTTTGG AGCTGGTGGA TATGGTCAGC TGGGACACAA CTCCACAAAC 
CATGAGGTCA ACCCCAGAAA GGTGTTTGAG CTCATGGGGA GCGTGGTTTC ACAGATTGCA 
TGTGGTCGGC AGCACACTCT GGCGTTCATC CCTTCATCAG GAAAGATAGA TTCGTTCGGT 
CTCGGGGGAA ACGGTCAGCT GGGAACGCGC TCCACCTGCA ACCGCATCAG TCCCACACCG 
GTGAAGGGCT GCTGGAGAGC CCACGCTGAC CCTGTTGCCA TGGATCATAG CGAGGCGGAG 
CGGAGTTACT GTGTGAAGAG GATTTATGCT GGAGGAGACC AGAGTTTCGC TCACTACGCT 
TTATCAGAGG ATGACGGTCC CCCACTGGAT GTGAGGATAA CCAGACCTGC TGGACAGATC 
TACACACTCA GCACTGAACT CATCCAGAAG TGGCTCAATC ACGGCCTTGG CAGGATACCA 
GCAGAAATCA CAGATGAGAT TGATCTTGTG TTTTCTTCTG CGAGTTGTCT CAATGGAAGC 
TTTCTGTCTT CAAGTAATCC AGATCACTAC AGCACGAGCA GTAAGTGCTC AGGTGTGGAC 
ATTAACACGG CTCGTCTGCT CTTCCACAAA CTCATCCAAA CCGATCACCC ACAAATCTCA 
CAAATCATCG CTGCTAGTTT GGAGAAGAAT CTCCTTCCCA AATTGAGCAG TTCTCCTCCA 
GACATCGAGG CTCTGAGACT GTACCTGACC CTGCCTGAGT GTCCACTTCT CTGCAACCCG 
AACAACTTCA CTACGTTAAC TATTCCCTTC GCCAAAGCCA TCGTCAGCCT GAAAGATGCT 
CCTATCAAAG TGCTCGGGAA CTGGTGGTCC CGGTTTGAGC CGGTGGTTTT CCAGAGGCTG 
GTGGAGCTCT ATAAGGAGGT GGTGGTGTAT CTGTTGCGCA TGCAGAAACT GGGCATTCCT 
CTTTCTGAAC AGAGGATCTT CACCTGCTTT TTAGACACTT CACTTAAACT GCTGGAGATC 
CTGCACTCGG TCAATGAGCG TGCCGGACAG ATCATCCAGT ATGATAAATT CTACATCCAT 
GAGCTAGACG AGCTGATCGA CATCAGGAAT GATTATGCGA CCTGGTTTCA GCAGCAGATG 
TTCTCTGTGG TCATGGACTC TCAGGTCACC CTCTGCAAAT ACCCGTTTGT GTTTGACGCT 
CAGGCGAAGA CGGCTCTGCT GCAGACTGAT GCTGTGATCC AGATGCAGAT GGCGGTGGAT 
CAGGCGCAGA GACAGAACTT CACCTCATTA TTTCTGTCCG GCGGAGGCGT GGAGTCAGTG 
AACCCCTGCC TCATCCTCAT CGTACGCAGA GAAAACGTGG TCGGAGACTC CATGGAGGTG 
CTGAGGAAGT CCAAAAACGT AGACTACAAG AAGCCACTCA AGGTGATCTT TGTTGGCGAG 
GAGGCTGTGG ACGCTGGTGG TGTGAGGAAA GAGTTTTTCC TGCTCATCAT GAAAGAGCTT 
CTGCACCCGA AATATGGCAT GTTTAGATTC CACGAGGAGT CCAGGCTTAT CTGGTTTGCT 
TGCAAGACGT TTGAAGATAT CGACCTGTTT CACCTGATCG GTGTTGTCTG TGGATTGGCC 
ATCTATAATC TCACTATAGT GGAGCTGAAT TTCCCGCTAG CGCTGTACAA GAAACTGCTG 
AAGAAAACAC CAACACTAGA GGACCTGAAG GAGCTCATGC CTGATGTGGG AAGGAGTCTT 
CAGCAGCTGC TGGATTATAC TGAAGATGAT TTGGAGGAAA CCTTCTGCCT GAATTTCACA 
ATCACAGAAG ATAATTTTGG AGCCACAGAG GTAGTGGAAC TGATCCCCAA CGGGACCGAC 
ATCAGCGTCA ACAAATCCAA CAGGCAGGAG TTTGTCAACG CTTATGTGGA TTATATATTC 
AACAAGTCTG TGGCTCCTCA GTTCAGTGCT TTCTATGCTG GATTTCACAA AGTGTGTGGA 
GGGAAAGTCC TGGAGCTTTT CCAGCCCAGT GAACTGCAGG CCATGGTCAT CGGAAACACC 
AACTATGACT GGAAGGAGCT GGAAAAGAGC ACCGAGTATA AGGGCGAGTA CTGGGCTGAT 
CATCCCACCA TCAAGATATT CTGGGAGGTT TTTCACGAGC TGCCTTTGGA GAAGAAGAAA 
CAGTTCCTGT TGTTCCTGAC AGGCAGTGAT CGTATCCCCA TTCTGGGCAT GAAGAGTCTG 
GCTCTGGTGA TTCAGCCGAC CGCAGGAGGA GAGCAGTATT TCCCAGTGGC ACACACATGC 
TTTAACCTGC TGGATCTGCC CAAATACACC AGCAAAAACA CAATGCAGGA GAAGCTGCTG 
CACGCCATTG AACACAACCA GGGCTTTAAC CTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173092.1
CDS	245..3403
Translation	MLCWGNASFGQLGLGGIDEEVLLEPRRCEFFEGKRVRDVGCGRRHTVFLLEDGTVYTCGCNDLGQLGHEKARKKPEQVVSLDAQNIVAVSCGEAHTLALNDKGQVFAWGLGSDGQLGLSIVEDCLRVPRTVKSLSEVHITQVACGYWHSLALARGGEIFSWGQNKYGQLGLGQQGACVCSPQVIQSLQGIPFGQISAGGAHSFGLTLSGAIFGWGRNKFGQLGLGDNDDRRFPVLLKTLRSQQVVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGSVVSQIACGRQHTLAFIPSSGKIDSFGLGGNGQLGTRSTCNRISPTPVKGCWRAHADPVAMDHSEAERSYCVKRIYAGGDQSFAHYALSEDDGPPLDVRITRPAGQIYTLSTELIQKWLNHGLGRIPAEITDEIDLVFSSASCLNGSFLSSSNPDHYSTSSKCSGVDINTARLLFHKLIQTDHPQISQIIAASLEKNLLPKLSSSPPDIEALRLYLTLPECPLLCNPNNFTTLTIPFAKAIVSLKDAPIKVLGNWWSRFEPVVFQRLVELYKEVVVYLLRMQKLGIPLSEQRIFTCFLDTSLKLLEILHSVNERAGQIIQYDKFYIHELDELIDIRNDYATWFQQQMFSVVMDSQVTLCKYPFVFDAQAKTALLQTDAVIQMQMAVDQAQRQNFTSLFLSGGGVESVNPCLILIVRRENVVGDSMEVLRKSKNVDYKKPLKVIFVGEEAVDAGGVRKEFFLLIMKELLHPKYGMFRFHEESRLIWFACKTFEDIDLFHLIGVVCGLAIYNLTIVELNFPLALYKKLLKKTPTLEDLKELMPDVGRSLQQLLDYTEDDLEETFCLNFTITEDNFGATEVVELIPNGTDISVNKSNRQEFVNAYVDYIFNKSVAPQFSAFYAGFHKVCGGKVLELFQPSELQAMVIGNTNYDWKELEKSTEYKGEYWADHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLALVIQPTAGGEQYFPVAHTCFNLLDLPKYTSKNTMQEKLLHAIEHNQGFNLV

Target ORF information:

RefSeq Version	XM_005173035.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HECT and RLD domain containing E3 ubiquitin protein ligase 4 (herc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173035.3

ORF Insert Sequence:

ATGTTATGTT GGGGAAACGC CTCCTTCGGG CAGCTGGGTT TAGGAGGTAT CGATGAGGAG 
GTGCTGCTGG AGCCCCGAAG ATGTGAGTTT TTCGAGGGTA AGCGCGTGCG GGACGTGGGC 
TGCGGGAGAC GCCACACTGT CTTCCTGCTA GAGGATGGGA CGGTTTACAC CTGCGGCTGC 
AATGACCTGG GGCAGCTGGG GCATGAGAAA GCCCGCAAGA AACCAGAGCA GGTGGTGTCT 
CTGGATGCGC AGAACATTGT GGCGGTGTCG TGTGGAGAAG CTCATACTCT TGCTCTGAAT 
GATAAAGGAC AAGTGTTTGC GTGGGGTTTG GGATCAGATG GACAGCTCGG ACTTTCCATT 
GTAGAAGACT GTCTGCGTGT GCCCAGAACT GTCAAGAGCT TGTCAGAGGT TCACATTACC 
CAGGTGGCCT GTGGATACTG GCATTCCCTT GCTTTAGCCC GAGGAGGCGA GATATTCTCC 
TGGGGCCAGA ATAAGTATGG TCAGCTGGGT CTGGGGCAGC AGGGAGCCTG TGTCTGCTCT 
CCACAGGTCA TCCAGTCTCT GCAGGGCATC CCGTTCGGGC AGATTTCCGC CGGTGGAGCT 
CACAGCTTTG GCCTCACGCT CTCTGGGGCC ATTTTCGGAT GGGGGCGGAA CAAGTTCGGC 
CAGCTGGGCC TCGGAGACAA TGATGATCGC CGTTTCCCAG TCTTGCTGAA GACTCTCAGG 
TCTCAACAGG TTGTTTACAT CTGCTGTGGA GAGGACCACA CGGCAGCGCT AACAAAGGAG 
GGCGGTGTAT TTACTTTTGG AGCTGGTGGA TATGGTCAGC TGGGACACAA CTCCACAAAC 
CATGAGGTCA ACCCCAGAAA GGTGTTTGAG CTCATGGGGA GCGTGGTTTC ACAGATTGCA 
TGTGGTCGGC AGCACACTCT GGCGTTCATC CCTTCATCAG GAAAGATAGA TTCGTTCGGT 
CTCGGGGGAA ACGGTCAGCT GGGAACGCGC TCCACCTGCA ACCGCATCAG TCCCACACCG 
GTGAAGGGCT GCTGGAGAGC CCACGCTGAC CCTGTTGCCA TGGATCATAG CGAGGCGGAG 
CGGAGTTACT GTGTGAAGAG GATTTATGCT GGAGGAGACC AGAGTTTCGC TCACTACGCT 
TTATCAGAGG ATGACGGTCC CCCACTGGAT GTGAGGATAA CCAGACCTGC TGGACAGATC 
TACACACTCA GCACTGAACT CATCCAGAAG TGGCTCAATC ACGGCCTTGG CAGGATACCA 
GCAGAAATCA CAGATGAGAT TGATCTTGTG TTTTCTTCTG CGAGTTGTCT CAATGGAAGC 
TTTCTGTCTT CAAGTAATCC AGATCACTAC AGCACGAGCA GTAAGTGCTC AGGTGTGGAC 
ATTAACACGG CTCGTCTGCT CTTCCACAAA CTCATCCAAA CCGATCACCC ACAAATCTCA 
CAAATCATCG CTGCTAGTTT GGAGAAGAAT CTCCTTCCCA AATTGAGCAG TTCTCCTCCA 
GACATCGAGG CTCTGAGACT GTACCTGACC CTGCCTGAGT GTCCACTTCT CTGCAACCCG 
AACAACTTCA CTACGTTAAC TATTCCCTTC GCCAAAGCCA TCGTCAGCCT GAAAGATGCT 
CCTATCAAAG TGCTCGGGAA CTGGTGGTCC CGGTTTGAGC CGGTGGTTTT CCAGAGGCTG 
GTGGAGCTCT ATAAGGAGGT GGTGGTGTAT CTGTTGCGCA TGCAGAAACT GGGCATTCCT 
CTTTCTGAAC AGAGGATCTT CACCTGCTTT TTAGACACTT CACTTAAACT GCTGGAGATC 
CTGCACTCGG TCAATGAGCG TGCCGGACAG ATCATCCAGT ATGATAAATT CTACATCCAT 
GAGCTAGACG AGCTGATCGA CATCAGGAAT GATTATGCGA CCTGGTTTCA GCAGCAGATG 
TTCTCTGTGG TCATGGACTC TCAGGTCACC CTCTGCAAAT ACCCGTTTGT GTTTGACGCT 
CAGGCGAAGA CGGCTCTGCT GCAGACTGAT GCTGTGATCC AGATGCAGAT GGCGGTGGAT 
CAGGCGCAGA GACAGAACTT CACCTCATTA TTTCTGTCCG GCGGAGGCGT GGAGTCAGTG 
AACCCCTGCC TCATCCTCAT CGTACGCAGA GAAAACGTGG TCGGAGACTC CATGGAGGTG 
CTGAGGAAGT CCAAAAACGT AGACTACAAG AAGCCACTCA AGGTGATCTT TGTTGGCGAG 
GAGGCTGTGG ACGCTGGTGG TGTGAGGAAA GAGTTTTTCC TGCTCATCAT GAAAGAGCTT 
CTGCACCCGA AATATGGCAT GTTTAGATTC CACGAGGAGT CCAGGCTTAT CTGGTTTGCT 
TGCAAGACGT TTGAAGATAT CGACCTGTTT CACCTGATCG GTGTTGTCTG TGGATTGGCC 
ATCTATAATC TCACTATAGT GGAGCTGAAT TTCCCGCTAG CGCTGTACAA GAAACTGCTG 
AAGAAAACAC CAACACTAGA GGACCTGAAG GAGCTCATGC CTGATGTGGG AAGGAGTCTT 
CAGCAGCTGC TGGATTATAC TGAAGATGAT TTGGAGGAAA CCTTCTGCCT GAATTTCACA 
ATCACAGAAG ATAATTTTGG AGCCACAGAG GTAGTGGAAC TGATCCCCAA CGGGACCGAC 
ATCAGCGTCA ACAAATCCAA CAGGCAGGAG TTTGTCAACG CTTATGTGGA TTATATATTC 
AACAAGTCTG TGGCTCCTCA GTTCAGTGCT TTCTATGCTG GATTTCACAA AGTGTGTGGA 
GGGAAAGTCC TGGAGCTTTT CCAGCCCAGT GAACTGCAGG CCATGGTCAT CGGAAACACC 
AACTATGACT GGAAGGAGCT GGAAAAGAGC ACCGAGTATA AGGGCGAGTA CTGGGCTGAT 
CATCCCACCA TCAAGATATT CTGGGAGGTT TTTCACGAGC TGCCTTTGGA GAAGAAGAAA 
CAGTTCCTGT TGTTCCTGAC AGGCAGTGAT CGTATCCCCA TTCTGGGCAT GAAGAGTCTG 
GCTCTGGTGA TTCAGCCGAC CGCAGGAGGA GAGCAGTATT TCCCAGTGGC ACACACATGC 
TTTAACCTGC TGGATCTGCC CAAATACACC AGCAAAAACA CAATGCAGGA GAAGCTGCTG 
CACGCCATTG AACACAACCA GGGCTTTAAC CTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HERC4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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