map3k5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	map3k5
Entrez Gene ID	555703
Full Name	mitogen-activated protein kinase kinase kinase 5
Synonyms	Ask1
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 5 Names mitogen-activated protein kinase kinase kinase 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001161750.1	NP_001155222.1	mitogen-activated protein kinase kinase kinase 5
XM_021468422.1	XP_021324097.1	mitogen-activated protein kinase kinase kinase 5 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAP3K5	XP_001171211.1
Macaca mulatta (Rhesus monkey)	MAP3K5	XP_002803972.1
Mus musculus (house mouse)	Map3k5	NP_032606.4
Danio rerio (zebrafish)	map3k5	NP_001155222.1
Homo sapiens (human)	MAP3K5	NP_005914.1
Rattus norvegicus (Norway rat)	Map3k5	NP_001264623.1
Gallus gallus (chicken)	MAP3K5	XP_419725.3
Xenopus tropicalis (tropical clawed frog)	LOC100497692	XP_002936311.2
Canis lupus familiaris (dog)	MAP3K5	XP_533420.4
Bos taurus (cattle)	MAP3K5	NP_001137553.1

Gene Ontology
Bibliography

Related articles in PubMed

The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection.
Yang D, Liu X, Xu W, Gu Z, Yang C, Zhang L, Tan J, Zheng X, Wang Z, Quan S, Zhang Y, Liu Q
PLoS pathogens15(7)e1007917(2019 07)

Aloe emodin induces hepatotoxicity by activating NF-κB inflammatory pathway and P53 apoptosis pathway in zebrafish.
Quan Y, Gong L, He J, Zhou Y, Liu M, Cao Z, Li Y, Peng C
Toxicology letters30666-79(2019 May)

Zimmerman MW, Liu Y, He S, Durbin AD, Abraham BJ, Easton J, Shao Y, Xu B, Zhu S, Zhang X, Li Z, Weichert-Leahey N, Young RA, Zhang J, Look AT
Cancer discovery8(3)320-335(2018 03)

Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake.
Pu L, Zhang LC, Zhang JS, Song X, Wang LG, Liang J, Zhang YB, Liu X, Yan H, Zhang T, Yue JW, Li N, Wu QQ, Wang LX
Genetics and molecular research : GMR15(3)(2016 Aug)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following map3k5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map3k5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36494	NM_001161750.1 Latest version!	Danio rerio mitogen-activated protein kinase kinase kinase 5 (map3k5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa150618	XM_021468422.1 Latest version!	Danio rerio mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

map3k5 ( NM_001161750.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001155222.1 Danio rerio mitogen-activated protein kinase kinase kinase 5 (map3k5), mRNA.

Price & Availability↑

CloneID	ODa36494
Clone ID Related Accession (Same CDS sequence)	NM_001161750.1
Accession Version	NM_001161750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575648000000
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase kinase kinase 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX248523.8. On Jun 13, 2009 this sequence version replaced XM_678295.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAATTCAG ATCTGGAGGC CATTTCTCTG TCTGTGCCCT CTCTCAGCCA GCCCCTGGAG 
ATGATGATGG TGATGATGGG AGGCAGTGAT GGAGAATCCA GTGCTGGGAA TAATTCTAGT 
GCTGGGGCTT TCTGGCAGGA CCCCTCCAAC TGTAAGAGTG CCAACCCCGG GCTGATCAGC 
TTGCCCTCGG ACGGGGTCGC ACTGCTGACC CCGGGGAAAT GCAAGAGCCG AGCCGTGACA 
GTGGCTTATG TGAGGAACTC CGAGACCAGC CAGCCTTCAA GCCCGGAGAA CATGGCACTA 
CAGTGTCTGA AGGATGCCTG CGACATTGTG GGCTGTAAAC TAGATGTGGT GCCCTTTGGG 
AAGCTGGACT TTGGCGAAAC ATCAGTTCTT GATCACTTTT ATAACGCAGA TATTGCAGTG 
GTTGAATTGA CGGATGCATT TCGGCAGCCC TCCCTCTTCT ATCATTTGGG TGTGAGAGAG 
AGTTTCTGCA TGGTCAACAA CATCATCCTT TACTGTGATC ACAACTCGGA CTCGCTGCAG 
TCTCTCCAGG ATTTGATTTG TCAGAAGAAT ATGACCTGCG CTGCTAACTA CACGTTTATC 
CCGTACATGG TGACGCCTCA GAGTAAGGTG TACTGCTGCG AGAGCAGTCT GATGAAGGGT 
TTGAGTGAAC TGATGCAGCC CAGCTTTGAG GCCCTGCTGG GGCCCATCTG TATGCCTCTG 
CTGGACAGAC TCACGCAGCT GCTCACCGGC TGCAAGTCAA ACACTTGCCA GTACTTCCGT 
GAGACCGTCC TGAACGAGAT TCGTAAAGCG CGTGAGCTTT ACACTGGAGC AGAGCTGGCG 
GCAGAACTCC GGCGCATCCA GCAGCGGCTC GACAACGTGG AGTGTCTCTC AGCAGACGTC 
GTCATCAATC TCCTGCTGTC ATACAGAGAT ATTCAGGATT ATGAATCTAT TGTGAAGTTA 
GTTGAAACAC TGGAAAAGCT GCCGACCTTT GACCCCGTGG CCCATCCTCA TATAAAGTTC 
CACTACGCAT TTGCCCTCAA CAGGAGAAAC CTACCTGGAG ACCGTCAGAA GGCGCTGGAC 
ATTATGTTGC CATTGGTCAA CTCCGATGAG CAGGTGGCGT CAGATATTTA TTGTTTGGTA 
GGCCGTATAT ATAAGGATAT GTTCCTGGAC TCTCATTTCA CAGACACAAA CAGCAGAGAT 
CAAGGCATAC ACTGGTTCAG AAGAGGGTTT GAATCGGAGC CCACGCTGCA CTCTGGTATC 
AACTATGCAG TGCTGCTGCT GGCTGCAGGA CATCAGTTTG ACTCCTCTTT TGAGCTGCGT 
AAAGTAGGTG TGAAGTTAAG CAGCCTGCTG GGGAAGAAAG GCAGTTTGGA CAGATTGCAG 
AGCTACTGGG ATGTGGGATT CTTTCTCGGG GCTAGTATTC TGGCCTGTGA CAACACTCGA 
GTCATCCAGG CCTCTGAGAA GCTCTTCAAA ATCAAAGCTC CCATTTGGTA TCTGCGCTCG 
CTGGTTGAAA CCATCCTCAT TTATAAGCAC TTTACCAAAG TAAGCACAGA TCCGCAGGCC 
CCTAAACAGG AGCTGGTGGA CTTCTGGATG GACTTCATGG TAGAAGCCAC CAATAAGGAT 
AAGGATGTGA CATCTGTGCG ATTTCCGGTG TTGATATTGG AGCCCACTAA AGTATACCAA 
CCGTCCTACC TCTCCATAAA CAAAGATGCT GAGGAGAACA CGTTATCCAT CTGGCACGTG 
ACCCCTGATG ACAAAAAAGG GATCCACGAG TGGAATTTCA GTGCAGCGTC TGTACGAGGT 
GTGAGCATCT CCAAATTTGA CGAGCGCAGC TGTTTTCTCT ACGTTGTGAA CAACTCTGAA 
GACTTCCAAA TTTATTTCTG CACAGAGATG CACTGCAAGA GGTTCTGTGA TCTGGTGAAC 
ACTGTCACTG AGGAGGCCTG GAAAGGCTCG GATGAGAACG AGTGTGAATC TGAGGCTTTA 
GAGTATGATT ATGAGTACGA TGAGCATGGA GAGAAGGTGG TTCTGGGTAA GGGCACCTTT 
GGAGTTGTCT ACGCTGGCCG TGACCTCAGT AACCAGGTGC GCTTAGCCAT CAAAGAGATA 
CCTGAACGGG ACAGCAGATA TTCACAGCCT CTGCATGAAG AAATCGCCCT GCATAAACAC 
CTCAAGCACA AGAACATCGT GCAGTACCTC GGCTCCATCA GCGAGAACGG CTTCATCAAG 
ATCTTCATGG AGCAGGTTCC TGGAGGCAGC CTGTCAGCAT TGCTTCGTTC AAAGTGGGGT 
CCTCTAAAAA ACAATGAGCC CACCATTGGC TTCTACACTA AGCAGATTCT GGATGGTCTT 
AAATACCTGC ATGACAACCA GATCGTTCAC AGGGACATTA AGGGGGATAA TGTCTTGATA 
AACACCTACA GTGGAGTTTT GAAAATCTCC GATTTTGGAA CGTCCAAACG TCTGGCTGGA 
ATAAACCCCT GCACTGAAAC ATTTACTGGT ACATTGCAGT ACATGGCCCC TGAAATCATA 
GATAAAGGAC CCCGTGGCTA CGGTAAGCCA GCAGACATCT GGTCCCTGGG TTGCACCATT 
ATAGAAATGG CCACTGGGAA ACCACCCTTC TATGAGCTGG GGGAACCTCA GGCAGCTATG 
TTCAAGGTTG GGATGTTTAA AATCCACCCG GAGATTCCTG ACTCCATGTC GTCTGAAGCC 
AAAGCCTTTA TCCTGCGCTG CTTTGAGCCT GATCCAGACA GCCGAGCCAC GGCCAACGAT 
CTGCTCACCC ATGAGTTCCT CACCGTCACA TCCCGCAAAA AACGATCCAA GACCAGCAGT 
TTCACAGCTC TTACACCAGG GTCCGAGTAT CTGCGCAGCA TTTCTCTGCC TGTTCCCGTG 
GTGGTGGAGG ACACCAGCAG CAGCAGCGAG TACGGCTCCG TCTCACCCGA CAATGAGCTC 
TGCACAAACC CCTTCAACTT CAGGCCCAGC AGCAGCAGCA AGAGCTACAG CGAGAGGGAG 
GTCAAAGGTC ATCGCTCGCT CTTCTTGAGT ATTCCTGTGG AGAACTTTGA AGACCACAGC 
GCACCACCGT CCCCGGAAGA GAAGGACTCC GGGTTCTTCA TGCTGAAGAA GGACAGTGAA 
AGAAGAGCGA CTTTACACCG GATCCTGACT GAGGACAGAG AGAAGATAGT GCTTCACCTG 
TTGGAGGCGC TCACACAGGG CTCTGAGGAG ACCAAACTGA AGCACCAGCA TATCTCCACA 
CTCGTGGCAA GCCTGGGAGA TTTCGTGAGG ATGGCGGACA GAAAAATAAT CGCCAGCACA 
CTTTCTCAGC TCAAACTGGA GCTGGACTTT GACAGCACAG CCATCAGCCA ACTGCAGGTG 
GCGCTCTTCG GCTTCCAGGA TGCGGTGAAT AAAGTCCTCC GCAACCACAA CATCAAGCCA 
CACTGGATGT TTGCCCTGGA TAACATCATT CGAAAGGCTG TGCAGACTGC CATCGTCATA 
TTGGTACCTG AATTGCGTCC ACACTTCACA CTGGCATCAG AGAGTGACCC CGCCGAGCAG 
GAGGACATCG ATGATGATGC AGTTCCTCAG GGAAACGCTG CACTAAAGGA CATGGCACCA 
GCACTCGTTC ATCACGACGA CACTGTGGCC ACGTCTGGAG TGAGCACGCT TAGTTCAACA 
GTTTCCCACG AGTCGCAGAG CGCGCAGAGG TCTATCAGCA TGGAGCTGGG TCGCATGAAG 
CTAGAAACCA AAAGGTTGTT GGAGGAATTA GTAGAAAAGG AGCGAGAATA TCAGGCCATC 
CTGCAGCAGG TCCTAGAGGA CAGAGAACAG GAGATCAAAC TATTAAAGTG TCGAACCGGA 
CCCATCGATG CGCCCGTGCA GCTCGTGAAC ACCCGCAGCT CATTGGATGG AGACGGAGAC 
CTGCAACTTC TGGAGTGGCT CAAACTCCAC GGGGCTGATG CTGATTCCAT AGAGAGGATA 
ATAGCTGAAG ATTACAGTCT TGATGACATC CTTCATTATG TCACCAGAGA CGATTTGAAG 
ACTTTGAGGT TACGAGGTGG AGTCCTGTGT AAACTGTGGA GCGCCATCAC AGAGCACAGA 
AAAAAGCCCG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155222.1
CDS	1..4095
Translation	MNSDLEAISLSVPSLSQPLEMMMVMMGGSDGESSAGNNSSAGAFWQDPSNCKSANPGLISLPSDGVALLTPGKCKSRAVTVAYVRNSETSQPSSPENMALQCLKDACDIVGCKLDVVPFGKLDFGETSVLDHFYNADIAVVELTDAFRQPSLFYHLGVRESFCMVNNIILYCDHNSDSLQSLQDLICQKNMTCAANYTFIPYMVTPQSKVYCCESSLMKGLSELMQPSFEALLGPICMPLLDRLTQLLTGCKSNTCQYFRETVLNEIRKARELYTGAELAAELRRIQQRLDNVECLSADVVINLLLSYRDIQDYESIVKLVETLEKLPTFDPVAHPHIKFHYAFALNRRNLPGDRQKALDIMLPLVNSDEQVASDIYCLVGRIYKDMFLDSHFTDTNSRDQGIHWFRRGFESEPTLHSGINYAVLLLAAGHQFDSSFELRKVGVKLSSLLGKKGSLDRLQSYWDVGFFLGASILACDNTRVIQASEKLFKIKAPIWYLRSLVETILIYKHFTKVSTDPQAPKQELVDFWMDFMVEATNKDKDVTSVRFPVLILEPTKVYQPSYLSINKDAEENTLSIWHVTPDDKKGIHEWNFSAASVRGVSISKFDERSCFLYVVNNSEDFQIYFCTEMHCKRFCDLVNTVTEEAWKGSDENECESEALEYDYEYDEHGEKVVLGKGTFGVVYAGRDLSNQVRLAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSISENGFIKIFMEQVPGGSLSALLRSKWGPLKNNEPTIGFYTKQILDGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKPADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKIHPEIPDSMSSEAKAFILRCFEPDPDSRATANDLLTHEFLTVTSRKKRSKTSSFTALTPGSEYLRSISLPVPVVVEDTSSSSEYGSVSPDNELCTNPFNFRPSSSSKSYSEREVKGHRSLFLSIPVENFEDHSAPPSPEEKDSGFFMLKKDSERRATLHRILTEDREKIVLHLLEALTQGSEETKLKHQHISTLVASLGDFVRMADRKIIASTLSQLKLELDFDSTAISQLQVALFGFQDAVNKVLRNHNIKPHWMFALDNIIRKAVQTAIVILVPELRPHFTLASESDPAEQEDIDDDAVPQGNAALKDMAPALVHHDDTVATSGVSTLSSTVSHESQSAQRSISMELGRMKLETKRLLEELVEKEREYQAILQQVLEDREQEIKLLKCRTGPIDAPVQLVNTRSSLDGDGDLQLLEWLKLHGADADSIERIIAEDYSLDDILHYVTRDDLKTLRLRGGVLCKLWSAITEHRKKPG

Target ORF information:

RefSeq Version	NM_001161750.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 5 (map3k5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161750.1

ORF Insert Sequence:

ATGAATTCAG ATCTGGAGGC CATTTCTCTG TCTGTGCCCT CTCTCAGCCA GCCCCTGGAG 
ATGATGATGG TGATGATGGG AGGCAGTGAT GGAGAATCCA GTGCTGGGAA TAATTCTAGT 
GCTGGGGCTT TCTGGCAGGA CCCCTCCAAC TGTAAGAGTG CCAACCCCGG GCTGATCAGC 
TTGCCCTCGG ACGGGGTCGC ACTGCTGACC CCGGGGAAAT GCAAGAGCCG AGCCGTGACA 
GTGGCTTATG TGAGGAACTC CGAGACCAGC CAGCCTTCAA GCCCGGAGAA CATGGCACTA 
CAGTGTCTGA AGGATGCCTG CGACATTGTG GGCTGTAAAC TAGATGTGGT GCCCTTTGGG 
AAGCTGGACT TTGGCGAAAC ATCAGTTCTT GATCACTTTT ATAACGCAGA TATTGCAGTG 
GTTGAATTGA CGGATGCATT TCGGCAGCCC TCCCTCTTCT ATCATTTGGG TGTGAGAGAG 
AGTTTCTGCA TGGTCAACAA CATCATCCTT TACTGTGATC ACAACTCGGA CTCGCTGCAG 
TCTCTCCAGG ATTTGATTTG TCAGAAGAAT ATGACCTGCG CTGCTAACTA CACGTTTATC 
CCGTACATGG TGACGCCTCA GAGTAAGGTG TACTGCTGCG AGAGCAGTCT GATGAAGGGT 
TTGAGTGAAC TGATGCAGCC CAGCTTTGAG GCCCTGCTGG GGCCCATCTG TATGCCTCTG 
CTGGACAGAC TCACGCAGCT GCTCACCGGC TGCAAGTCAA ACACTTGCCA GTACTTCCGT 
GAGACCGTCC TGAACGAGAT TCGTAAAGCG CGTGAGCTTT ACACTGGAGC AGAGCTGGCG 
GCAGAACTCC GGCGCATCCA GCAGCGGCTC GACAACGTGG AGTGTCTCTC AGCAGACGTC 
GTCATCAATC TCCTGCTGTC ATACAGAGAT ATTCAGGATT ATGAATCTAT TGTGAAGTTA 
GTTGAAACAC TGGAAAAGCT GCCGACCTTT GACCCCGTGG CCCATCCTCA TATAAAGTTC 
CACTACGCAT TTGCCCTCAA CAGGAGAAAC CTACCTGGAG ACCGTCAGAA GGCGCTGGAC 
ATTATGTTGC CATTGGTCAA CTCCGATGAG CAGGTGGCGT CAGATATTTA TTGTTTGGTA 
GGCCGTATAT ATAAGGATAT GTTCCTGGAC TCTCATTTCA CAGACACAAA CAGCAGAGAT 
CAAGGCATAC ACTGGTTCAG AAGAGGGTTT GAATCGGAGC CCACGCTGCA CTCTGGTATC 
AACTATGCAG TGCTGCTGCT GGCTGCAGGA CATCAGTTTG ACTCCTCTTT TGAGCTGCGT 
AAAGTAGGTG TGAAGTTAAG CAGCCTGCTG GGGAAGAAAG GCAGTTTGGA CAGATTGCAG 
AGCTACTGGG ATGTGGGATT CTTTCTCGGG GCTAGTATTC TGGCCTGTGA CAACACTCGA 
GTCATCCAGG CCTCTGAGAA GCTCTTCAAA ATCAAAGCTC CCATTTGGTA TCTGCGCTCG 
CTGGTTGAAA CCATCCTCAT TTATAAGCAC TTTACCAAAG TAAGCACAGA TCCGCAGGCC 
CCTAAACAGG AGCTGGTGGA CTTCTGGATG GACTTCATGG TAGAAGCCAC CAATAAGGAT 
AAGGATGTGA CATCTGTGCG ATTTCCGGTG TTGATATTGG AGCCCACTAA AGTATACCAA 
CCGTCCTACC TCTCCATAAA CAAAGATGCT GAGGAGAACA CGTTATCCAT CTGGCACGTG 
ACCCCTGATG ACAAAAAAGG GATCCACGAG TGGAATTTCA GTGCAGCGTC TGTACGAGGT 
GTGAGCATCT CCAAATTTGA CGAGCGCAGC TGTTTTCTCT ACGTTGTGAA CAACTCTGAA 
GACTTCCAAA TTTATTTCTG CACAGAGATG CACTGCAAGA GGTTCTGTGA TCTGGTGAAC 
ACTGTCACTG AGGAGGCCTG GAAAGGCTCG GATGAGAACG AGTGTGAATC TGAGGCTTTA 
GAGTATGATT ATGAGTACGA TGAGCATGGA GAGAAGGTGG TTCTGGGTAA GGGCACCTTT 
GGAGTTGTCT ACGCTGGCCG TGACCTCAGT AACCAGGTGC GCTTAGCCAT CAAAGAGATA 
CCTGAACGGG ACAGCAGATA TTCACAGCCT CTGCATGAAG AAATCGCCCT GCATAAACAC 
CTCAAGCACA AGAACATCGT GCAGTACCTC GGCTCCATCA GCGAGAACGG CTTCATCAAG 
ATCTTCATGG AGCAGGTTCC TGGAGGCAGC CTGTCAGCAT TGCTTCGTTC AAAGTGGGGT 
CCTCTAAAAA ACAATGAGCC CACCATTGGC TTCTACACTA AGCAGATTCT GGATGGTCTT 
AAATACCTGC ATGACAACCA GATCGTTCAC AGGGACATTA AGGGGGATAA TGTCTTGATA 
AACACCTACA GTGGAGTTTT GAAAATCTCC GATTTTGGAA CGTCCAAACG TCTGGCTGGA 
ATAAACCCCT GCACTGAAAC ATTTACTGGT ACATTGCAGT ACATGGCCCC TGAAATCATA 
GATAAAGGAC CCCGTGGCTA CGGTAAGCCA GCAGACATCT GGTCCCTGGG TTGCACCATT 
ATAGAAATGG CCACTGGGAA ACCACCCTTC TATGAGCTGG GGGAACCTCA GGCAGCTATG 
TTCAAGGTTG GGATGTTTAA AATCCACCCG GAGATTCCTG ACTCCATGTC GTCTGAAGCC 
AAAGCCTTTA TCCTGCGCTG CTTTGAGCCT GATCCAGACA GCCGAGCCAC GGCCAACGAT 
CTGCTCACCC ATGAGTTCCT CACCGTCACA TCCCGCAAAA AACGATCCAA GACCAGCAGT 
TTCACAGCTC TTACACCAGG GTCCGAGTAT CTGCGCAGCA TTTCTCTGCC TGTTCCCGTG 
GTGGTGGAGG ACACCAGCAG CAGCAGCGAG TACGGCTCCG TCTCACCCGA CAATGAGCTC 
TGCACAAACC CCTTCAACTT CAGGCCCAGC AGCAGCAGCA AGAGCTACAG CGAGAGGGAG 
GTCAAAGGTC ATCGCTCGCT CTTCTTGAGT ATTCCTGTGG AGAACTTTGA AGACCACAGC 
GCACCACCGT CCCCGGAAGA GAAGGACTCC GGGTTCTTCA TGCTGAAGAA GGACAGTGAA 
AGAAGAGCGA CTTTACACCG GATCCTGACT GAGGACAGAG AGAAGATAGT GCTTCACCTG 
TTGGAGGCGC TCACACAGGG CTCTGAGGAG ACCAAACTGA AGCACCAGCA TATCTCCACA 
CTCGTGGCAA GCCTGGGAGA TTTCGTGAGG ATGGCGGACA GAAAAATAAT CGCCAGCACA 
CTTTCTCAGC TCAAACTGGA GCTGGACTTT GACAGCACAG CCATCAGCCA ACTGCAGGTG 
GCGCTCTTCG GCTTCCAGGA TGCGGTGAAT AAAGTCCTCC GCAACCACAA CATCAAGCCA 
CACTGGATGT TTGCCCTGGA TAACATCATT CGAAAGGCTG TGCAGACTGC CATCGTCATA 
TTGGTACCTG AATTGCGTCC ACACTTCACA CTGGCATCAG AGAGTGACCC CGCCGAGCAG 
GAGGACATCG ATGATGATGC AGTTCCTCAG GGAAACGCTG CACTAAAGGA CATGGCACCA 
GCACTCGTTC ATCACGACGA CACTGTGGCC ACGTCTGGAG TGAGCACGCT TAGTTCAACA 
GTTTCCCACG AGTCGCAGAG CGCGCAGAGG TCTATCAGCA TGGAGCTGGG TCGCATGAAG 
CTAGAAACCA AAAGGTTGTT GGAGGAATTA GTAGAAAAGG AGCGAGAATA TCAGGCCATC 
CTGCAGCAGG TCCTAGAGGA CAGAGAACAG GAGATCAAAC TATTAAAGTG TCGAACCGGA 
CCCATCGATG CGCCCGTGCA GCTCGTGAAC ACCCGCAGCT CATTGGATGG AGACGGAGAC 
CTGCAACTTC TGGAGTGGCT CAAACTCCAC GGGGCTGATG CTGATTCCAT AGAGAGGATA 
ATAGCTGAAG ATTACAGTCT TGATGACATC CTTCATTATG TCACCAGAGA CGATTTGAAG 
ACTTTGAGGT TACGAGGTGG AGTCCTGTGT AAACTGTGGA GCGCCATCAC AGAGCACAGA 
AAAAAGCCCG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k5 ( XM_021468422.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324097.1 Danio rerio mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa150618
Clone ID Related Accession (Same CDS sequence)	XM_021468422.1
Accession Version	XM_021468422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCAG ATCTGGAGGC CATTTCTCTG TCTGTGCCCT CTCTCAGCCA GCCCCTGGAG 
ATGATGATGG TGATGATGGG AGGCAGTGAT GGAGAATCCA GTGCTGGGAA TAATTCTAGT 
GCTGGGGCTT TCTGGCAGGA CCCCTCCAAC TGTAAGAGTG CCAACCCCGG GCTGATCAGC 
TTGCCCTCGG ACGGGGTCGC ACTGCTGACC CCGGGGAAAT GCAAGAGCCG AGCCGTGACA 
GTGGCTTATG TGAGGAACTC CGAGACCAGC CAGCCTTCAA GCCCGGAGAA CATGGCACTA 
CAGTGTCTGA AGGATGCCTG CGACATTGTG GGCTGTAAAC TAGATGTGGT GCCCTTTGGG 
AAGCTGGACT TTGGCGAAAC ATCAGTTCTT GATCACTTTT ATAACGCAGA TATTGCAGTG 
GTTGAATTGA CGGATGCATT TCGGCAGCCC TCCCTCTTCT ATCATTTGGG TGTGAGAGAG 
AGTTTCTGCA TGGTCAACAA CATCATCCTT TACTGTGATC ACAACTCGGA CTCGCTGCAG 
TCTCTCCAGA CCTGCGCTGC TAACTACACG TTTATCCCGT ACATGGTGAC GCCTCAGAGT 
AAGGTGTACT GCTGCGAGAG CAGTCTGATG AAGGGTTTGA GTGAACTGAT GCAGCCCAGC 
TTTGAGGCCC TGCTGGGGCC CATCTGTATG CCTCTGCTGG ACAGACTCAC GCAGCTGCTC 
ACCGGCTGCA AGTCAAACAC TTGCCAGTAC TTCCGTGAGA CCGTCCTGAA CGAGATTCGT 
AAAGCGCGTG AGCTTTACAC TGGAGCAGAG CTGGCGGCAG AACTCCGGCG CATCCAGCAG 
CGGCTCGACA ACGTGGAGTG TCTCTCAGCA GACGTCGTCA TCAATCTCCT GCTGTCATAC 
AGAGATATTC AGGATTATGA ATCTATTGTG AAGTTAGTTG AAACACTGGA AAAGCTGCCG 
ACCTTTGACC CCGTGGCCCA TCCTCATATA AAGTTCCACT ACGCATTTGC CCTCAACAGG 
AGAAACCTAC CTGGAGACCG TCAGAAGGCG CTGGACATTA TGTTGCCATT GGTCAACTCC 
GATGAGCAGG TGGCGTCAGA TATTTATTGT TTGGTAGGCC GTATATATAA GGATATGTTC 
CTGGACTCTC ATTTCACAGA CACAAACAGC AGAGATCAAG GCATACACTG GTTCAGAAGA 
GGGTTTGAAT CGGAGCCCAC GCTGCACTCT GGTATCAACT ATGCAGTGCT GCTGCTGGCT 
GCAGGACATC AGTTTGACTC CTCTTTTGAG CTGCGTAAAG TAGGTGTGAA GTTAAGCAGC 
CTGCTGGGGA AGAAAGGCAG TTTGGACAGA TTGCAGAGCT ACTGGGATGT GGGATTCTTT 
CTCGGGGCTA GTATTCTGGC CTGTGACAAC ACTCGAGTCA TCCAGGCCTC TGAGAAGCTC 
TTCAAAATCA AAGCTCCCAT TTGGTATCTG CGCTCGCTGG TTGAAACCAT CCTCATTTAT 
AAGCACTTTA CCAAAGTAAG CACAGATCCG CAGGCCCCTA AACAGGAGCT GGTGGACTTC 
TGGATGGACT TCATGGTAGA AGCCACCAAT AAGGATAAGG ATGTGACATC TGTGCGATTT 
CCGGTGTTGA TATTGGAGCC CACTAAAGTA TACCAACCGT CCTACCTCTC CATAAACAAA 
GATGCTGAGG AGAACACGTT ATCCATCTGG CACGTGACCC CTGATGACAA AAAAGGGATC 
CACGAGTGGA ATTTCAGTGC AGCGTCTGTA CGAGGTGTGA GCATCTCCAA ATTTGACGAG 
CGCAGCTGTT TTCTCTACGT TGTGAACAAC TCTGAAGACT TCCAAATTTA TTTCTGCACA 
GAGATGCACT GCAAGAGGTT CTGTGATCTG GTGAACACTG TCACTGAGGA GGCCTGGAAA 
GGCTCGGATG AGAACGAGTG TGAATCTGAG GCTTTAGAGT ATGATTATGA GTACGATGAG 
CATGGAGAGA AGGTGGTTCT GGGTAAGGGC ACCTTTGGAG TTGTCTACGC TGGCCGTGAC 
CTCAGTAACC AGGTGCGCTT AGCCATCAAA GAGATACCTG AACGGGACAG CAGATATTCA 
CAGCCTCTGC ATGAAGAAAT CGCCCTGCAT AAACACCTCA AGCACAAGAA CATCGTGCAG 
TACCTCGGCT CCATCAGCGA GAACGGCTTC ATCAAGATCT TCATGGAGCA GGTTCCTGGA 
GGCAGCCTGT CAGCATTGCT TCGTTCAAAG TGGGGTCCTC TAAAAAACAA TGAGCCCACC 
ATTGGCTTCT ACACTAAGCA GATTCTGGAT GGTCTTAAAT ACCTGCATGA CAACCAGATC 
GTTCACAGGG ACATTAAGGG GGATAATGTC TTGATAAACA CCTACAGTGG AGTTTTGAAA 
ATCTCCGATT TTGGAACGTC CAAACGTCTG GCTGGAATAA ACCCCTGCAC TGAAACATTT 
ACTGGTACAT TGCAGTACAT GGCCCCTGAA ATCATAGATA AAGGACCCCG TGGCTACGGT 
AAGCCAGCAG ACATCTGGTC CCTGGGTTGC ACCATTATAG AAATGGCCAC TGGGAAACCA 
CCCTTCTATG AGCTGGGGGA ACCTCAGGCA GCTATGTTCA AGGTTGGGAT GTTTAAAATC 
CACCCGGAGA TTCCTGACTC CATGTCGTCT GAAGCCAAAG CCTTTATCCT GCGCTGCTTT 
GAGCCTGATC CAGACAGCCG AGCCACGGCC AACGATCTGC TCACCCATGA GTTCCTCACC 
GTCACATCCC GCAAAAAACG ATCCAAGACC AGCAGTTTCA CAGCTCTTAC ACCAGGGTCC 
GAGTATCTGC GCAGCATTTC TCTGCCTGTT CCCGTGGTGG TGGAGGACAC CAGCAGCAGC 
AGCGAGTACG GCTCCGTCTC ACCCGACAAT GAGCTCTGCA CAAACCCCTT CAACTTCAGG 
CCCAGCAGCA GCAGCAAGAG CTACAGCGAG AGGGAGGTCA AAGGTCATCG CTCGCTCTTC 
TTGAGTATTC CTGTGGAGAA CTTTGAAGAC CACAGCGCAC CACCGTCCCC GGAAGAGAAG 
GACTCCGGGT TCTTCATGCT GAAGAAGGAC AGTGAAAGAA GAGCGACTTT ACACCGGATC 
CTGACTGAGG ACAGAGAGAA GATAGTGCTT CACCTGTTGG AGGCGCTCAC ACAGGGCTCT 
GAGGAGACCA AACTGAAGCA CCAGCATATC TCCACACTCG TGGCAAGCCT GGGAGATTTC 
GTGAGGATGG CGGACAGAAA AATAATCGCC AGCACACTTT CTCAGCTCAA ACTGGAGCTG 
GACTTTGACA GCACAGCCAT CAGCCAACTG CAGGTGGCGC TCTTCGGCTT CCAGGATGCG 
GTGAATAAAG TCCTCCGCAA CCACAACATC AAGCCACACT GGATGTTTGC CCTGGATAAC 
ATCATTCGAA AGGCTGTGCA GACTGCCATC GTCATATTGG TACCTGAATT GCGTCCACAC 
TTCACACTGG CATCAGAGAG TGACCCCGCC GAGCAGGAGG ACATCGATGA TGATGCAGTT 
CCTCAGGGAA ACGCTGCACT AAAGGACATG GCACCAGCAC TCGTTCATCA CGACGACACT 
GTGGCCACGT CTGGAGTGAG CACGCTTAGT TCAACAGTTT CCCACGAGTC GCAGAGCGCG 
CAGAGGTCTA TCAGCATGGA GCTGGGTCGC ATGAAGCTAG AAACCAAAAG GTTGTTGGAG 
GAATTAGTAG AAAAGGAGCG AGAATATCAG GCCATCCTGC AGCAGGTCCT AGAGGACAGA 
GAACAGGAGA TCAAACTATT AAAGTGTCGA ACCGGACCCA TCGATGCGCC CGTGCAGCTC 
GTGAACACCC GCAGCTCATT GGATGGAGAC GGAGACCTGC AACTTCTGGA GTGGCTCAAA 
CTCCACGGGG CTGATGCTGA TTCCATAGAG AGGATAATAG CTGAAGATTA CAGTCTTGAT 
GACATCCTTC ATTATGTCAC CAGAGACGAT TTGAAGACTT TGAGGTTACG AGGTGGAGTC 
CTGTGTAAAC TGTGGAGCGC CATCACAGAG CACAGAAAAA AGCCCGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324097.1
CDS	601..4671
Translation	MNSDLEAISLSVPSLSQPLEMMMVMMGGSDGESSAGNNSSAGAFWQDPSNCKSANPGLISLPSDGVALLTPGKCKSRAVTVAYVRNSETSQPSSPENMALQCLKDACDIVGCKLDVVPFGKLDFGETSVLDHFYNADIAVVELTDAFRQPSLFYHLGVRESFCMVNNIILYCDHNSDSLQSLQTCAANYTFIPYMVTPQSKVYCCESSLMKGLSELMQPSFEALLGPICMPLLDRLTQLLTGCKSNTCQYFRETVLNEIRKARELYTGAELAAELRRIQQRLDNVECLSADVVINLLLSYRDIQDYESIVKLVETLEKLPTFDPVAHPHIKFHYAFALNRRNLPGDRQKALDIMLPLVNSDEQVASDIYCLVGRIYKDMFLDSHFTDTNSRDQGIHWFRRGFESEPTLHSGINYAVLLLAAGHQFDSSFELRKVGVKLSSLLGKKGSLDRLQSYWDVGFFLGASILACDNTRVIQASEKLFKIKAPIWYLRSLVETILIYKHFTKVSTDPQAPKQELVDFWMDFMVEATNKDKDVTSVRFPVLILEPTKVYQPSYLSINKDAEENTLSIWHVTPDDKKGIHEWNFSAASVRGVSISKFDERSCFLYVVNNSEDFQIYFCTEMHCKRFCDLVNTVTEEAWKGSDENECESEALEYDYEYDEHGEKVVLGKGTFGVVYAGRDLSNQVRLAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSISENGFIKIFMEQVPGGSLSALLRSKWGPLKNNEPTIGFYTKQILDGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKPADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKIHPEIPDSMSSEAKAFILRCFEPDPDSRATANDLLTHEFLTVTSRKKRSKTSSFTALTPGSEYLRSISLPVPVVVEDTSSSSEYGSVSPDNELCTNPFNFRPSSSSKSYSEREVKGHRSLFLSIPVENFEDHSAPPSPEEKDSGFFMLKKDSERRATLHRILTEDREKIVLHLLEALTQGSEETKLKHQHISTLVASLGDFVRMADRKIIASTLSQLKLELDFDSTAISQLQVALFGFQDAVNKVLRNHNIKPHWMFALDNIIRKAVQTAIVILVPELRPHFTLASESDPAEQEDIDDDAVPQGNAALKDMAPALVHHDDTVATSGVSTLSSTVSHESQSAQRSISMELGRMKLETKRLLEELVEKEREYQAILQQVLEDREQEIKLLKCRTGPIDAPVQLVNTRSSLDGDGDLQLLEWLKLHGADADSIERIIAEDYSLDDILHYVTRDDLKTLRLRGGVLCKLWSAITEHRKKPG

Target ORF information:

RefSeq Version	XM_021468422.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase 5 (map3k5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468422.1

ORF Insert Sequence:

ATGAATTCAG ATCTGGAGGC CATTTCTCTG TCTGTGCCCT CTCTCAGCCA GCCCCTGGAG 
ATGATGATGG TGATGATGGG AGGCAGTGAT GGAGAATCCA GTGCTGGGAA TAATTCTAGT 
GCTGGGGCTT TCTGGCAGGA CCCCTCCAAC TGTAAGAGTG CCAACCCCGG GCTGATCAGC 
TTGCCCTCGG ACGGGGTCGC ACTGCTGACC CCGGGGAAAT GCAAGAGCCG AGCCGTGACA 
GTGGCTTATG TGAGGAACTC CGAGACCAGC CAGCCTTCAA GCCCGGAGAA CATGGCACTA 
CAGTGTCTGA AGGATGCCTG CGACATTGTG GGCTGTAAAC TAGATGTGGT GCCCTTTGGG 
AAGCTGGACT TTGGCGAAAC ATCAGTTCTT GATCACTTTT ATAACGCAGA TATTGCAGTG 
GTTGAATTGA CGGATGCATT TCGGCAGCCC TCCCTCTTCT ATCATTTGGG TGTGAGAGAG 
AGTTTCTGCA TGGTCAACAA CATCATCCTT TACTGTGATC ACAACTCGGA CTCGCTGCAG 
TCTCTCCAGA CCTGCGCTGC TAACTACACG TTTATCCCGT ACATGGTGAC GCCTCAGAGT 
AAGGTGTACT GCTGCGAGAG CAGTCTGATG AAGGGTTTGA GTGAACTGAT GCAGCCCAGC 
TTTGAGGCCC TGCTGGGGCC CATCTGTATG CCTCTGCTGG ACAGACTCAC GCAGCTGCTC 
ACCGGCTGCA AGTCAAACAC TTGCCAGTAC TTCCGTGAGA CCGTCCTGAA CGAGATTCGT 
AAAGCGCGTG AGCTTTACAC TGGAGCAGAG CTGGCGGCAG AACTCCGGCG CATCCAGCAG 
CGGCTCGACA ACGTGGAGTG TCTCTCAGCA GACGTCGTCA TCAATCTCCT GCTGTCATAC 
AGAGATATTC AGGATTATGA ATCTATTGTG AAGTTAGTTG AAACACTGGA AAAGCTGCCG 
ACCTTTGACC CCGTGGCCCA TCCTCATATA AAGTTCCACT ACGCATTTGC CCTCAACAGG 
AGAAACCTAC CTGGAGACCG TCAGAAGGCG CTGGACATTA TGTTGCCATT GGTCAACTCC 
GATGAGCAGG TGGCGTCAGA TATTTATTGT TTGGTAGGCC GTATATATAA GGATATGTTC 
CTGGACTCTC ATTTCACAGA CACAAACAGC AGAGATCAAG GCATACACTG GTTCAGAAGA 
GGGTTTGAAT CGGAGCCCAC GCTGCACTCT GGTATCAACT ATGCAGTGCT GCTGCTGGCT 
GCAGGACATC AGTTTGACTC CTCTTTTGAG CTGCGTAAAG TAGGTGTGAA GTTAAGCAGC 
CTGCTGGGGA AGAAAGGCAG TTTGGACAGA TTGCAGAGCT ACTGGGATGT GGGATTCTTT 
CTCGGGGCTA GTATTCTGGC CTGTGACAAC ACTCGAGTCA TCCAGGCCTC TGAGAAGCTC 
TTCAAAATCA AAGCTCCCAT TTGGTATCTG CGCTCGCTGG TTGAAACCAT CCTCATTTAT 
AAGCACTTTA CCAAAGTAAG CACAGATCCG CAGGCCCCTA AACAGGAGCT GGTGGACTTC 
TGGATGGACT TCATGGTAGA AGCCACCAAT AAGGATAAGG ATGTGACATC TGTGCGATTT 
CCGGTGTTGA TATTGGAGCC CACTAAAGTA TACCAACCGT CCTACCTCTC CATAAACAAA 
GATGCTGAGG AGAACACGTT ATCCATCTGG CACGTGACCC CTGATGACAA AAAAGGGATC 
CACGAGTGGA ATTTCAGTGC AGCGTCTGTA CGAGGTGTGA GCATCTCCAA ATTTGACGAG 
CGCAGCTGTT TTCTCTACGT TGTGAACAAC TCTGAAGACT TCCAAATTTA TTTCTGCACA 
GAGATGCACT GCAAGAGGTT CTGTGATCTG GTGAACACTG TCACTGAGGA GGCCTGGAAA 
GGCTCGGATG AGAACGAGTG TGAATCTGAG GCTTTAGAGT ATGATTATGA GTACGATGAG 
CATGGAGAGA AGGTGGTTCT GGGTAAGGGC ACCTTTGGAG TTGTCTACGC TGGCCGTGAC 
CTCAGTAACC AGGTGCGCTT AGCCATCAAA GAGATACCTG AACGGGACAG CAGATATTCA 
CAGCCTCTGC ATGAAGAAAT CGCCCTGCAT AAACACCTCA AGCACAAGAA CATCGTGCAG 
TACCTCGGCT CCATCAGCGA GAACGGCTTC ATCAAGATCT TCATGGAGCA GGTTCCTGGA 
GGCAGCCTGT CAGCATTGCT TCGTTCAAAG TGGGGTCCTC TAAAAAACAA TGAGCCCACC 
ATTGGCTTCT ACACTAAGCA GATTCTGGAT GGTCTTAAAT ACCTGCATGA CAACCAGATC 
GTTCACAGGG ACATTAAGGG GGATAATGTC TTGATAAACA CCTACAGTGG AGTTTTGAAA 
ATCTCCGATT TTGGAACGTC CAAACGTCTG GCTGGAATAA ACCCCTGCAC TGAAACATTT 
ACTGGTACAT TGCAGTACAT GGCCCCTGAA ATCATAGATA AAGGACCCCG TGGCTACGGT 
AAGCCAGCAG ACATCTGGTC CCTGGGTTGC ACCATTATAG AAATGGCCAC TGGGAAACCA 
CCCTTCTATG AGCTGGGGGA ACCTCAGGCA GCTATGTTCA AGGTTGGGAT GTTTAAAATC 
CACCCGGAGA TTCCTGACTC CATGTCGTCT GAAGCCAAAG CCTTTATCCT GCGCTGCTTT 
GAGCCTGATC CAGACAGCCG AGCCACGGCC AACGATCTGC TCACCCATGA GTTCCTCACC 
GTCACATCCC GCAAAAAACG ATCCAAGACC AGCAGTTTCA CAGCTCTTAC ACCAGGGTCC 
GAGTATCTGC GCAGCATTTC TCTGCCTGTT CCCGTGGTGG TGGAGGACAC CAGCAGCAGC 
AGCGAGTACG GCTCCGTCTC ACCCGACAAT GAGCTCTGCA CAAACCCCTT CAACTTCAGG 
CCCAGCAGCA GCAGCAAGAG CTACAGCGAG AGGGAGGTCA AAGGTCATCG CTCGCTCTTC 
TTGAGTATTC CTGTGGAGAA CTTTGAAGAC CACAGCGCAC CACCGTCCCC GGAAGAGAAG 
GACTCCGGGT TCTTCATGCT GAAGAAGGAC AGTGAAAGAA GAGCGACTTT ACACCGGATC 
CTGACTGAGG ACAGAGAGAA GATAGTGCTT CACCTGTTGG AGGCGCTCAC ACAGGGCTCT 
GAGGAGACCA AACTGAAGCA CCAGCATATC TCCACACTCG TGGCAAGCCT GGGAGATTTC 
GTGAGGATGG CGGACAGAAA AATAATCGCC AGCACACTTT CTCAGCTCAA ACTGGAGCTG 
GACTTTGACA GCACAGCCAT CAGCCAACTG CAGGTGGCGC TCTTCGGCTT CCAGGATGCG 
GTGAATAAAG TCCTCCGCAA CCACAACATC AAGCCACACT GGATGTTTGC CCTGGATAAC 
ATCATTCGAA AGGCTGTGCA GACTGCCATC GTCATATTGG TACCTGAATT GCGTCCACAC 
TTCACACTGG CATCAGAGAG TGACCCCGCC GAGCAGGAGG ACATCGATGA TGATGCAGTT 
CCTCAGGGAA ACGCTGCACT AAAGGACATG GCACCAGCAC TCGTTCATCA CGACGACACT 
GTGGCCACGT CTGGAGTGAG CACGCTTAGT TCAACAGTTT CCCACGAGTC GCAGAGCGCG 
CAGAGGTCTA TCAGCATGGA GCTGGGTCGC ATGAAGCTAG AAACCAAAAG GTTGTTGGAG 
GAATTAGTAG AAAAGGAGCG AGAATATCAG GCCATCCTGC AGCAGGTCCT AGAGGACAGA 
GAACAGGAGA TCAAACTATT AAAGTGTCGA ACCGGACCCA TCGATGCGCC CGTGCAGCTC 
GTGAACACCC GCAGCTCATT GGATGGAGAC GGAGACCTGC AACTTCTGGA GTGGCTCAAA 
CTCCACGGGG CTGATGCTGA TTCCATAGAG AGGATAATAG CTGAAGATTA CAGTCTTGAT 
GACATCCTTC ATTATGTCAC CAGAGACGAT TTGAAGACTT TGAGGTTACG AGGTGGAGTC 
CTGTGTAAAC TGTGGAGCGC CATCACAGAG CACAGAAAAA AGCCCGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection.
PLoS pathogens15(7)e1007917(2019 07)
Yang D,Liu X,Xu W,Gu Z,Yang C,Zhang L,Tan J,Zheng X,Wang Z,Quan S,Zhang Y,Liu Q

Aloe emodin induces hepatotoxicity by activating NF-κB inflammatory pathway and P53 apoptosis pathway in zebrafish.
Toxicology letters30666-79(2019 May)
Quan Y,Gong L,He J,Zhou Y,Liu M,Cao Z,Li Y,Peng C

Cancer discovery8(3)320-335(2018 03)
Zimmerman MW,Liu Y,He S,Durbin AD,Abraham BJ,Easton J,Shao Y,Xu B,Zhu S,Zhang X,Li Z,Weichert-Leahey N,Young RA,Zhang J,Look AT

Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake.
Genetics and molecular research : GMR15(3)(2016 Aug)
Pu L,Zhang LC,Zhang JS,Song X,Wang LG,Liang J,Zhang YB,Liu X,Yan H,Zhang T,Yue JW,Li N,Wu QQ,Wang LX

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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map3k5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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