Map3k5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map3k5
Entrez Gene ID	26408
Full Name	mitogen-activated protein kinase kinase kinase 5
Synonyms	7420452D20Rik,ASK,ASK1,MAPKKK5,Mekk5
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 5 Names mitogen-activated protein kinase kinase kinase 5 ASK-1 MAPK/ERK kinase kinase 5 MEK kinase 5 MEKK 5 apoptosis signal-regulating kinase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006512739.3	XP_006512802.1	mitogen-activated protein kinase kinase kinase 5 isoform X1
XM_006512739.4	XP_006512802.1	mitogen-activated protein kinase kinase kinase 5 isoform X1
NM_008580.4	NP_032606.4	mitogen-activated protein kinase kinase kinase 5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAP3K5	XP_001171211.1
Macaca mulatta (Rhesus monkey)	MAP3K5	XP_002803972.1
Mus musculus (house mouse)	Map3k5	NP_032606.4
Danio rerio (zebrafish)	map3k5	NP_001155222.1
Homo sapiens (human)	MAP3K5	NP_005914.1
Rattus norvegicus (Norway rat)	Map3k5	NP_001264623.1
Gallus gallus (chicken)	MAP3K5	XP_419725.3
Xenopus tropicalis (tropical clawed frog)	LOC100497692	XP_002936311.2
Canis lupus familiaris (dog)	MAP3K5	XP_533420.4
Bos taurus (cattle)	MAP3K5	NP_001137553.1

Gene Ontology
Bibliography

Related articles in PubMed

miR-23b promotes cutaneous wound healing through inhibition of the inflammatory responses by targeting ASK1.
Li H, Han X, Zuo K, Li L, Liu J, Yuan X, Shen Y, Shao M, Pang D, Chu Y, Zhao B
Acta biochimica et biophysica Sinica50(11)1104-1113(2018 Nov)

β-TrCP-dependent degradation of ASK1 suppresses the induction of the apoptotic response by oxidative stress.
Cheng R, Takeda K, Naguro I, Hatta T, Iemura SI, Natsume T, Ichijo H, Hattori K
Biochimica et biophysica acta. General subjects1862(10)2271-2280(2018 10)

Regulator of G Protein Signaling 6 Facilities Cardiac Hypertrophy by Activating Apoptosis Signal-Regulating Kinase 1-P38/c-JUN N-Terminal Kinase 1/2 Signaling.
Huang Z, Shu J, Jiang W, Jiang M, Lu Y, Dai H, Xu N, Yuan H, Cai J
Journal of the American Heart Association7(19)e009179(2018 10)

ASK1 contributes to fibrosis and dysfunction in models of kidney disease.
Liles JT, Corkey BK, Notte GT, Budas GR, Lansdon EB, Hinojosa-Kirschenbaum F, Badal SS, Lee M, Schultz BE, Wise S, Pendem S, Graupe M, Castonguay L, Koch KA, Wong MH, Papalia GA, French DM, Sullivan T, Huntzicker EG, Ma FY, Nikolic-Paterson DJ, Altuhaifi T, Yang H, Fogo AB, Breckenridge DG
The Journal of clinical investigation128(10)4485-4500(2018 10)

Apoptosis Signal-regulating Kinase 1 Silencing on Astroglial Inflammasomes in an Experimental Model of Ischemic Stroke.
Cheon SY, Kim EJ, Kim SY, Kim JM, Kam EH, Park JK, Koo BN
Neuroscience390218-230(2018 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Map3k5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map3k5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21432	NM_008580.4 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 5 (Map3k5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu43874	XM_006512739.4 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 5 (Map3k5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu43874	XM_006512739.3	Mus musculus mitogen-activated protein kinase kinase kinase 5 (Map3k5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map3k5 ( NM_008580.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032606.4 Mus musculus mitogen-activated protein kinase kinase kinase 5 (Map3k5), mRNA.

Price & Availability↑

CloneID	OMu21432
Clone ID Related Accession (Same CDS sequence)	NM_008580.4
Accession Version	NM_008580.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574092800000
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC133697.1, AC171277.9, AC158621.9, AK078668.1 and AC158620.6. On Apr 2, 2008 this sequence version replaced NM_008580.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC116627.1, AB006787.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGGCACCG AAGCCGGCGA GGGCATCACT TTCTCCGTGC CGCCCTTTGC GTCCGTGGGC 
TTTTGCACCA TCCCGGAGGG CGGCAGCTGC CGGAGGGGAG GAGGAGCGGC GACGGCGGCG 
GAGGGAGAGC CGAGCCTGCA GCCCTTGCTT GTGCCGCCGC CGCCGCCACC GCCGGGCAGT 
TTCTGGAACG TGGAGAGCGC GGCGGCTCCC GGCACCAGCT GCCCTACCAC CGCCCCCGGG 
AGCAGCGCCA CCCGAGGCCG GGGCAACTCT GGCAGCGGGG GCGGCCGGCG TACCACCGTG 
GCTTATGTGA TCAATGAGGC GAGCCAGGGG CAGCTGGTGG TGGCGGAGAG CGAGGCCCTG 
CAGAGCCTGC GGGAGGCGTG CGAGGCGGTG GGCGCCACCC TGGAGACCCT GCATTTCGGG 
AAGCTGGACT TCGGGGAAAC CGCCGTGCTG GACCGTTTTT ACAACGCAGA TATTGCTGTG 
GTGGAGATGA GCGACGCCTT CCGGCAGCCT TCCTTGTTTT ACCATCTTGG AGTGCGAGAA 
AGCTTCAGTA TGGCCAACAA CATCATCCTC TACTGCGATA CTAATTCCGA TTCACTCCAG 
TCCCTGAAGG AAATTATTTG CCAGAAGAAT ACTGTGTGCA CCGGGAACTA CACCTTCATC 
CCCTACATGG TGACACCACA CAACAAGGTC TACTGCTGTG ACAGCAGCTT CATGAAGGGC 
CTGACTGAGC TCATGCAGCC TAACTTCGAG CTCCTGCTGG GGCCCATCTG CTTACCCCTC 
GTGGACCGTT TCGTTCAGCT TTTGAAGGTG GCGCAAGCGA GTTCCAGCCA GTACTTCCGG 
GAATCCATAC TCAGCGATAT CAGGAAAGCA CGGAACCTGT ACACTGGTAA AGAATTGGCA 
GCTGAATTGG CGAGGATCCG GCAACGGGTA GACAATATTG AAGTTTTGAC AGCAGACATT 
GTTATCAACC TGTTGCTTTC CTACAGAGAC ATCCAGGACT ATGACTCCAT AGTGAAGCTG 
GTAGAGACGT TAGAAAAACT TCCAACCTTC GACTTGGCCT CGCACCACCA CGTGAAGTTT 
CATTACGCAT TTGCACTGAA CAGGAGAAAT CTCCCTGGAG ACAGAGCAAA AGCTCTTGAC 
ATCATGATCC CCATGGTACA AAGTGAAGAG CAAGTTGCTT CGGATATGTA TTGTCTCGTT 
GGCCGAATCT ACAAAGATAT GTTTTTGGAT TCAAATTTCA CTGACACGGA AAGCAGGGAC 
CATGGAGCAT CTTGGTTCAA AAAGGCATTT GAGTCTGAGC CAACACTACA GTCAGGAATT 
AATTATGCAG TTCTCTTGCT GGCGGCTGGG CACCAGTTTG AATCTTCTTT TGAGCTTCGG 
AAAGTTGGAG TGAAGCTGAG TAGCCTTCTG GGTAAAAAGG GGAACTTGGA AAAACTCCAG 
AGCTACTGGG AAGTCGGATT TTTTCTGGGA GCCAGTGTCC TGGCCAACGA CCACCTCAGG 
GTCATTCAGG CATCCGAGAA GCTGTTCAGA CTGAAGACAC CAGCGTGGTA CCTCAAGTCT 
ATCGTGGAGA CCATTCTGAT ATATAAACAC TTTGTGAAAC TGACCACAGA GCAGCCCTCG 
GCTAAGCAGG AACTCGTGGA CTTCTGGATG GATTTCCTGG TCGAGGCCAC AAAAACAGAT 
GTTACTGTTG TCAGGTTCCC AGTATTAATA TTAGAGCCAA CCAAAATCTA TCAGCCTTCT 
TACCTGTCTA TCAATAATGA AGTTGAGGAG AAGACAATAT CTATTTGGCA TGTTCTCCCT 
GATGACAAGA AAGGTATACA CGAGTGGAAC TTCGGTGCTT CCTCTGTCAG GGGAGTGAGC 
ATCTCTAAGT TTGAAGAACG ATGCTGTTTC CTTTATGTGC TTCACAATTC TGATGACTTC 
CAAATCTACT TCTGCACAGA ACTTCACTGT AAAAGGTTTT TTGAAATGGT GAATACCATC 
ACGGAAGAGA AGGGGAGAGG CGCGGAGGAC GGAGACTGTG AGGGTGACTC CCTGGAGTAT 
GACTACGAAT ACGATGAAAA TGGAGACAGA GTTGTGTTAG GGAAGGGCAC TTATGGGATC 
GTCTATGCAG GACGAGACCT GAGCAACCAG GTCAGAATTG CTATTAAGGA AATCCCAGAG 
AGAGACAGCA GATACTCTCA GCCCCTGCAT GAAGAAATCG CCCTGCACAA GCATCTGAAA 
CACAAAAACA TTGTCCAGTA TCTGGGCTCC TTCAGTGAGA ACGGCTTCAT CAAAATCTTC 
ATGGAGCAAG TCCCAGGAGG AAGCCTCTCT GCTCTCCTTC GTTCAAAATG GGGCCCATTA 
AAGGACAACG AACAGACGAT TGGCTTCTAT ACGAAGCAGA TACTGGAAGG ATTAAAATAC 
CTTCACGACA ATCAGATAGT CCACCGGGAT ATAAAGGGTG ACAATGTATT GATTAATACC 
TACAGTGGTG TGCTCAAGAT CTCTGACTTC GGGACATCCA AGAGGCTTGC TGGCATAAAC 
CCATGTACCG AAACCTTTAC AGGTACCCTT CAGTATATGG CGCCAGAAAT AATCGATAAA 
GGACCACGAG GCTATGGAAA GGCCGCCGAC ATTTGGTCAC TGGGCTGCAC AATCATCGAA 
ATGGCTACTG GAAAACCACC ATTTTATGAA CTAGGAGAGC CACAGGCAGC CATGTTCAAG 
GTGGGGATGT TTAAGGTCCA CCCGGAGATC CCAGAGTCCA TGTCGGCGGA GGCCAAGGCG 
TTCATACTGA AGTGTTTTGA ACCAGACCCT GACAAGAGAG CCTGTGCTAA TGACTTGCTT 
ATTGATGAGT TCTTAAAAGT GTCCAGCAAA AAGAAAAAGA CACAACCCAA ACTTTCAGCT 
CTCTCGACTG GATCGAATGA GTATCTTAGA AGCATCTCCC TGCCGGTCCC TGTCCTGGTA 
GAAGACACCA GCAGCAGTAG CGAGTATGGC TCTGTTTCTC CTGATACAGA GCTGAAGGCG 
GACCCCTTCT CCTTCAAAGC CAGAGCTAAG TCCTGTGGAG AAAAGGACGG GAAAGGGATA 
CGGACATTGT TCCTGGGCAT TCCAGATGAA AATTTTGAAG ATCACAGTGC GCCCCCATCT 
CCTGAAGAGA AAGACTCGGG CTTTTTTATG CTGAGAAAGG ATAGTGAGAG GCGAGCTACC 
CTTCACAGAA TCCTGACCGA AGACCAGGAC AAGGTTGTAA GGAACCTAAT GGAATCTCTG 
GCCCAGGGTG CTGAAGAGCC TAAACTAAAG TGGGAACACA TCACAACCCT CATCTCGAGC 
CTCAGAGAGT TTGTGAGGTC CACTGACCGA AAAATCATAG CCACTACACT GTCCAAGCTC 
AAGCTGGAGC TGGACTTCGA CAGCCACGGC ATCAGCCAGG TGCAGGTGGT CCTCTTTGGC 
TTCCAGGATG CGGTCAATAA AGTTCTTCGG AATCATAACA TCAAGCCACA CTGGATGTTT 
GCCCTGGACA GCATCATCCG AAAGGCTGTG CAGACTGCAA TTACCATTCT GGTTCCAGAG 
TTGAGGCCAC ATTTTAGCCT TGCATCAGAG AGCGACACAG CTGATCCGGA AGACCTGGAT 
GTCGAAGATG AACACGAGGA ATTGTCTTCA AATCAGACAG TCCGACGGCC TCAGGCCATC 
ACTGAGGACG CTGTGGCTAC CTCGGGGGTG AGCACGCTAA GCTCCACTGT GTCCCATGAC 
TCCCAGAATG CACACCGGTC GCTGAACGTG CAGCTTGGAA GGATGAAGAT TGAAACTAAT 
AGGTTACTTG AAGAGCTGGT TCGGAAAGAG AGAGAGTTAC AGGCACTCCT CCATCAAGCC 
ATTGAGGAAA AAGACCAAGA AATTAGGCAC CTGAAGCTCA AGTCCCAGCC CATAGATATC 
CCTGGGTTTC CTGTGTGCCA CCTGAACTCT CCTGGCACGA CCACGGAAGA TTCTGAACTT 
CCTGGCTGGC TGAGAGAAAA TGGAGCTGAT GAAGACACTA TAAGTCGGTT TCTGGCTGAG 
GATTACACGC TAGTGGATGT TCTTTACTAC GTCACACGTG ATGATCTGAA GTGCCTCAGA 
CTAAGGGGCG GGATGCTGTG CACGCTGTGG AAGGCCATCA TTGACTTTCG GAACAAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032606.4
CDS	81..4223
Translation	MGTEAGEGITFSVPPFASVGFCTIPEGGSCRRGGGAATAAEGEPSLQPLLVPPPPPPPGSFWNVESAAAPGTSCPTTAPGSSATRGRGNSGSGGGRRTTVAYVINEASQGQLVVAESEALQSLREACEAVGATLETLHFGKLDFGETAVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTVCTGNYTFIPYMVTPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFVQLLKVAQASSSQYFRESILSDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEEQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHLRVIQASEKLFRLKTPAWYLKSIVETILIYKHFVKLTTEQPSAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFGASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKRFFEMVNTITEEKGRGAEDGDCEGDSLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLIDEFLKVSSKKKKTQPKLSALSTGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKADPFSFKARAKSCGEKDGKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKVVRNLMESLAQGAEEPKLKWEHITTLISSLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADPEDLDVEDEHEELSSNQTVRRPQAITEDAVATSGVSTLSSTVSHDSQNAHRSLNVQLGRMKIETNRLLEELVRKERELQALLHQAIEEKDQEIRHLKLKSQPIDIPGFPVCHLNSPGTTTEDSELPGWLRENGADEDTISRFLAEDYTLVDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKC

Target ORF information:

RefSeq Version	NM_008580.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 5 (Map3k5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008580.4

ORF Insert Sequence:

ATGGGCACCG AAGCCGGCGA GGGCATCACT TTCTCCGTGC CGCCCTTTGC GTCCGTGGGC 
TTTTGCACCA TCCCGGAGGG CGGCAGCTGC CGGAGGGGAG GAGGAGCGGC GACGGCGGCG 
GAGGGAGAGC CGAGCCTGCA GCCCTTGCTT GTGCCGCCGC CGCCGCCACC GCCGGGCAGT 
TTCTGGAACG TGGAGAGCGC GGCGGCTCCC GGCACCAGCT GCCCTACCAC CGCCCCCGGG 
AGCAGCGCCA CCCGAGGCCG GGGCAACTCT GGCAGCGGGG GCGGCCGGCG TACCACCGTG 
GCTTATGTGA TCAATGAGGC GAGCCAGGGG CAGCTGGTGG TGGCGGAGAG CGAGGCCCTG 
CAGAGCCTGC GGGAGGCGTG CGAGGCGGTG GGCGCCACCC TGGAGACCCT GCATTTCGGG 
AAGCTGGACT TCGGGGAAAC CGCCGTGCTG GACCGTTTTT ACAACGCAGA TATTGCTGTG 
GTGGAGATGA GCGACGCCTT CCGGCAGCCT TCCTTGTTTT ACCATCTTGG AGTGCGAGAA 
AGCTTCAGTA TGGCCAACAA CATCATCCTC TACTGCGATA CTAATTCCGA TTCACTCCAG 
TCCCTGAAGG AAATTATTTG CCAGAAGAAT ACTGTGTGCA CCGGGAACTA CACCTTCATC 
CCCTACATGG TGACACCACA CAACAAGGTC TACTGCTGTG ACAGCAGCTT CATGAAGGGC 
CTGACTGAGC TCATGCAGCC TAACTTCGAG CTCCTGCTGG GGCCCATCTG CTTACCCCTC 
GTGGACCGTT TCGTTCAGCT TTTGAAGGTG GCGCAAGCGA GTTCCAGCCA GTACTTCCGG 
GAATCCATAC TCAGCGATAT CAGGAAAGCA CGGAACCTGT ACACTGGTAA AGAATTGGCA 
GCTGAATTGG CGAGGATCCG GCAACGGGTA GACAATATTG AAGTTTTGAC AGCAGACATT 
GTTATCAACC TGTTGCTTTC CTACAGAGAC ATCCAGGACT ATGACTCCAT AGTGAAGCTG 
GTAGAGACGT TAGAAAAACT TCCAACCTTC GACTTGGCCT CGCACCACCA CGTGAAGTTT 
CATTACGCAT TTGCACTGAA CAGGAGAAAT CTCCCTGGAG ACAGAGCAAA AGCTCTTGAC 
ATCATGATCC CCATGGTACA AAGTGAAGAG CAAGTTGCTT CGGATATGTA TTGTCTCGTT 
GGCCGAATCT ACAAAGATAT GTTTTTGGAT TCAAATTTCA CTGACACGGA AAGCAGGGAC 
CATGGAGCAT CTTGGTTCAA AAAGGCATTT GAGTCTGAGC CAACACTACA GTCAGGAATT 
AATTATGCAG TTCTCTTGCT GGCGGCTGGG CACCAGTTTG AATCTTCTTT TGAGCTTCGG 
AAAGTTGGAG TGAAGCTGAG TAGCCTTCTG GGTAAAAAGG GGAACTTGGA AAAACTCCAG 
AGCTACTGGG AAGTCGGATT TTTTCTGGGA GCCAGTGTCC TGGCCAACGA CCACCTCAGG 
GTCATTCAGG CATCCGAGAA GCTGTTCAGA CTGAAGACAC CAGCGTGGTA CCTCAAGTCT 
ATCGTGGAGA CCATTCTGAT ATATAAACAC TTTGTGAAAC TGACCACAGA GCAGCCCTCG 
GCTAAGCAGG AACTCGTGGA CTTCTGGATG GATTTCCTGG TCGAGGCCAC AAAAACAGAT 
GTTACTGTTG TCAGGTTCCC AGTATTAATA TTAGAGCCAA CCAAAATCTA TCAGCCTTCT 
TACCTGTCTA TCAATAATGA AGTTGAGGAG AAGACAATAT CTATTTGGCA TGTTCTCCCT 
GATGACAAGA AAGGTATACA CGAGTGGAAC TTCGGTGCTT CCTCTGTCAG GGGAGTGAGC 
ATCTCTAAGT TTGAAGAACG ATGCTGTTTC CTTTATGTGC TTCACAATTC TGATGACTTC 
CAAATCTACT TCTGCACAGA ACTTCACTGT AAAAGGTTTT TTGAAATGGT GAATACCATC 
ACGGAAGAGA AGGGGAGAGG CGCGGAGGAC GGAGACTGTG AGGGTGACTC CCTGGAGTAT 
GACTACGAAT ACGATGAAAA TGGAGACAGA GTTGTGTTAG GGAAGGGCAC TTATGGGATC 
GTCTATGCAG GACGAGACCT GAGCAACCAG GTCAGAATTG CTATTAAGGA AATCCCAGAG 
AGAGACAGCA GATACTCTCA GCCCCTGCAT GAAGAAATCG CCCTGCACAA GCATCTGAAA 
CACAAAAACA TTGTCCAGTA TCTGGGCTCC TTCAGTGAGA ACGGCTTCAT CAAAATCTTC 
ATGGAGCAAG TCCCAGGAGG AAGCCTCTCT GCTCTCCTTC GTTCAAAATG GGGCCCATTA 
AAGGACAACG AACAGACGAT TGGCTTCTAT ACGAAGCAGA TACTGGAAGG ATTAAAATAC 
CTTCACGACA ATCAGATAGT CCACCGGGAT ATAAAGGGTG ACAATGTATT GATTAATACC 
TACAGTGGTG TGCTCAAGAT CTCTGACTTC GGGACATCCA AGAGGCTTGC TGGCATAAAC 
CCATGTACCG AAACCTTTAC AGGTACCCTT CAGTATATGG CGCCAGAAAT AATCGATAAA 
GGACCACGAG GCTATGGAAA GGCCGCCGAC ATTTGGTCAC TGGGCTGCAC AATCATCGAA 
ATGGCTACTG GAAAACCACC ATTTTATGAA CTAGGAGAGC CACAGGCAGC CATGTTCAAG 
GTGGGGATGT TTAAGGTCCA CCCGGAGATC CCAGAGTCCA TGTCGGCGGA GGCCAAGGCG 
TTCATACTGA AGTGTTTTGA ACCAGACCCT GACAAGAGAG CCTGTGCTAA TGACTTGCTT 
ATTGATGAGT TCTTAAAAGT GTCCAGCAAA AAGAAAAAGA CACAACCCAA ACTTTCAGCT 
CTCTCGACTG GATCGAATGA GTATCTTAGA AGCATCTCCC TGCCGGTCCC TGTCCTGGTA 
GAAGACACCA GCAGCAGTAG CGAGTATGGC TCTGTTTCTC CTGATACAGA GCTGAAGGCG 
GACCCCTTCT CCTTCAAAGC CAGAGCTAAG TCCTGTGGAG AAAAGGACGG GAAAGGGATA 
CGGACATTGT TCCTGGGCAT TCCAGATGAA AATTTTGAAG ATCACAGTGC GCCCCCATCT 
CCTGAAGAGA AAGACTCGGG CTTTTTTATG CTGAGAAAGG ATAGTGAGAG GCGAGCTACC 
CTTCACAGAA TCCTGACCGA AGACCAGGAC AAGGTTGTAA GGAACCTAAT GGAATCTCTG 
GCCCAGGGTG CTGAAGAGCC TAAACTAAAG TGGGAACACA TCACAACCCT CATCTCGAGC 
CTCAGAGAGT TTGTGAGGTC CACTGACCGA AAAATCATAG CCACTACACT GTCCAAGCTC 
AAGCTGGAGC TGGACTTCGA CAGCCACGGC ATCAGCCAGG TGCAGGTGGT CCTCTTTGGC 
TTCCAGGATG CGGTCAATAA AGTTCTTCGG AATCATAACA TCAAGCCACA CTGGATGTTT 
GCCCTGGACA GCATCATCCG AAAGGCTGTG CAGACTGCAA TTACCATTCT GGTTCCAGAG 
TTGAGGCCAC ATTTTAGCCT TGCATCAGAG AGCGACACAG CTGATCCGGA AGACCTGGAT 
GTCGAAGATG AACACGAGGA ATTGTCTTCA AATCAGACAG TCCGACGGCC TCAGGCCATC 
ACTGAGGACG CTGTGGCTAC CTCGGGGGTG AGCACGCTAA GCTCCACTGT GTCCCATGAC 
TCCCAGAATG CACACCGGTC GCTGAACGTG CAGCTTGGAA GGATGAAGAT TGAAACTAAT 
AGGTTACTTG AAGAGCTGGT TCGGAAAGAG AGAGAGTTAC AGGCACTCCT CCATCAAGCC 
ATTGAGGAAA AAGACCAAGA AATTAGGCAC CTGAAGCTCA AGTCCCAGCC CATAGATATC 
CCTGGGTTTC CTGTGTGCCA CCTGAACTCT CCTGGCACGA CCACGGAAGA TTCTGAACTT 
CCTGGCTGGC TGAGAGAAAA TGGAGCTGAT GAAGACACTA TAAGTCGGTT TCTGGCTGAG 
GATTACACGC TAGTGGATGT TCTTTACTAC GTCACACGTG ATGATCTGAA GTGCCTCAGA 
CTAAGGGGCG GGATGCTGTG CACGCTGTGG AAGGCCATCA TTGACTTTCG GAACAAATGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k5 ( XM_006512739.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512802.1 Mus musculus mitogen-activated protein kinase kinase kinase 5 (Map3k5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu43874
Clone ID Related Accession (Same CDS sequence)	XM_006512739.3 , XM_006512739.4
Accession Version	XM_006512739.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512739.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCG AAGCCGGCGA GGGCATCACT TTCTCCGTGC CGCCCTTTGC GTCCGTGGGC 
TTTTGCACCA TCCCGGAGGG CGGCAGCTGC CGGAGGGGAG GAGGAGCGGC GACGGCGGCG 
GAGGGAGAGC CGAGCCTGCA GCCCTTGCTT GTGCCGCCGC CGCCGCCACC GCCGGGCAGT 
TTCTGGAACG TGGAGAGCGC GGCGGCTCCC GGCACCAGCT GCCCTACCAC CGCCCCCGGG 
AGCAGCGCCA CCCGAGGCCG GGGCAACTCT GGCAGCGGGG GCGGCCGGCG TACCACCGTG 
GCTTATGTGA TCAATGAGGC GAGCCAGGGG CAGCTGGTGG TGGCGGAGAG CGAGGCCCTG 
CAGAGCCTGC GGGAGGCGTG CGAGGCGGTG GGCGCCACCC TGGAGACCCT GCATTTCGGG 
AAGCTGGACT TCGGGGAAAC CGCCGTGCTG GACCGTTTTT ACAACGCAGA TATTGCTGTG 
GTGGAGATGA GCGACGCCTT CCGGCAGCCT TCCTTGTTTT ACCATCTTGG AGTGCGAGAA 
AGCTTCAGTA TGGCCAACAA CATCATCCTC TACTGCGATA CTAATTCCGA TTCACTCCAG 
TCCCTGAAGG AAATTATTTG CCAGAAGAAT ACTGTGTGCA CCGGGAACTA CACCTTCATC 
CCCTACATGG TGACACCACA CAACAAGGTC TACTGCTGTG ACAGCAGCTT CATGAAGGGC 
CTGACTGAGC TCATGCAGCC TAACTTCGAG CTCCTGCTGG GGCCCATCTG CTTACCCCTC 
GTGGACCGTT TCGTTCAGCT TTTGAAGGTG GCGCAAGCGA GTTCCAGCCA GTACTTCCGG 
GAATCCATAC TCAGCGATAT CAGGAAAGCA CGGAACCTGT ACACTGGTAA AGAATTGGCA 
GCTGAATTGG CGAGGATCCG GCAACGGGTA GACAATATTG AAGTTTTGAC AGCAGACATT 
GTTATCAACC TGTTGCTTTC CTACAGAGAC ATCCAGGACT ATGACTCCAT AGTGAAGCTG 
GTAGAGACGT TAGAAAAACT TCCAACCTTC GACTTGGCCT CGCACCACCA CGTGAAGTTT 
CATTACGCAT TTGCACTGAA CAGGAGAAAT CTCCCTGGAG ACAGAGCAAA AGCTCTTGAC 
ATCATGATCC CCATGGTACA AAGTGAAGAG CAAGTTGCTT CGGATATGTA TTGTCTCGTT 
GGCCGAATCT ACAAAGATAT GTTTTTGGAT TCAAATTTCA CTGACACGGA AAGCAGGGAC 
CATGGAGCAT CTTGGTTCAA AAAGGCATTT GAGTCTGAGC CAACACTACA GTCAGGAATT 
AATTATGCAG TTCTCTTGCT GGCGGCTGGG CACCAGTTTG AATCTTCTTT TGAGCTTCGG 
AAAGTTGGAG TGAAGCTGAG TAGCCTTCTG GGTAAAAAGG GGAACTTGGA AAAACTCCAG 
AGCTACTGGG AAGTCGGATT TTTTCTGGGA GCCAGTGTCC TGGCCAACGA CCACCTCAGG 
GTCATTCAGG CATCCGAGAA GCTGTTCAGA CTGAAGACAC CAGCGTGGTA CCTCAAGTCT 
ATCGTGGAGA CCATTCTGAT ATATAAACAC TTTGTGAAAC TGACCACAGA GCAGCCCTCG 
GCTAAGCAGG AACTCGTGGA CTTCTGGATG GATTTCCTGG TCGAGGCCAC AAAAACAGAT 
GTTACTGTTG TCAGGTTCCC AGTATTAATA TTAGAGCCAA CCAAAATCTA TCAGCCTTCT 
TACCTGTCTA TCAATAATGA AGTTGAGGAG AAGACAATAT CTATTTGGCA TGTTCTCCCT 
GATGACAAGA AAGGTATACA CGAGTGGAAC TTCGGTGCTT CCTCTGTCAG GGGAGTGAGC 
ATCTCTAAGT TTGAAGAACG ATGCTGTTTC CTTTATGTGC TTCACAATTC TGATGACTTC 
CAAATCTACT TCTGCACAGA ACTTCACTGT AAAAGGTTTT TTGAAATGGT GAATACCATC 
ACGGAAGAGA AGGGGAGAGG CGCGGAGGAC GGAGACTGTG AGGGTGACTC CCTGGAGTAT 
GACTACGAAT ACGATGAAAA TGGAGACAGA GTTGTGTTAG GGAAGGGCAC TTATGGGATC 
GTCTATGCAG GACGAGACCT GAGCAACCAG GTCAGAATTG CTATTAAGGA AATCCCAGAG 
AGAGACAGCA GATACTCTCA GCCCCTGCAT GAAGAAATCG CCCTGCACAA GCATCTGAAA 
CACAAAAACA TTGTCCAGTA TCTGGGCTCC TTCAGTGAGA ACGGCTTCAT CAAAATCTTC 
ATGGAGCAAG TCCCAGGAGG AAGCCTCTCT GCTCTCCTTC GTTCAAAATG GGGCCCATTA 
AAGGACAACG AACAGACGAT TGGCTTCTAT ACGAAGCAGA TACTGGAAGG ATTAAAATAC 
CTTCACGACA ATCAGATAGT CCACCGGGAT ATAAAGGGTG ACAATGTATT GATTAATACC 
TACAGTGGTG TGCTCAAGAT CTCTGACTTC GGGACATCCA AGAGGCTTGC TGGCATAAAC 
CCATGTACCG AAACCTTTAC AGGTACCCTT CAGTATATGG CGCCAGAAAT AATCGATAAA 
GGACCACGAG GCTATGGAAA GGCCGCCGAC ATTTGGTCAC TGGGCTGCAC AATCATCGAA 
ATGGCTACTG GAAAACCACC ATTTTATGAA CTAGGAGAGC CACAGGCAGC CATGTTCAAG 
GTGGGGATGT TTAAGGTCCA CCCGGAGATC CCAGAGTCCA TGTCGGCGGA GGCCAAGGCG 
TTCATACTGA AGTGTTTTGA ACCAGACCCT GACAAGAGAG CCTGTGCTAA TGACTTGCTT 
ATTGATGAGT TCTTAAAAGT GTCCAGCAAA AAGAAAAAGA CACAACCCAA ACTTTCAGCT 
CTCTCGACTG GATCGAATGC CTTAATGACA GACAGCATCC ATCAGAGGCC AGGAGCCCAG 
GTGGGTCCTT TCCACCCAGC CGCCCCCTCT AGTCTATGTC ACATATGCAT GCCTTTGGTG 
GCTTCTGTCA TGAACGGGTC CTCTTCAGAC ATCCCCTTGC CTAGCGCTGT GCTTTTTCCC 
TCTCCGCCAT CATGGATTCA GGTCTGCAAG TTCACTATGC CCAGGTCTTC TCTGCTTGCT 
ATTTCTGGCA GTCTAGTATT TGCCCTTCCA CTTGACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512802.1
CDS	95..3256
Translation	MGTEAGEGITFSVPPFASVGFCTIPEGGSCRRGGGAATAAEGEPSLQPLLVPPPPPPPGSFWNVESAAAPGTSCPTTAPGSSATRGRGNSGSGGGRRTTVAYVINEASQGQLVVAESEALQSLREACEAVGATLETLHFGKLDFGETAVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTVCTGNYTFIPYMVTPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFVQLLKVAQASSSQYFRESILSDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEEQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHLRVIQASEKLFRLKTPAWYLKSIVETILIYKHFVKLTTEQPSAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFGASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKRFFEMVNTITEEKGRGAEDGDCEGDSLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLIDEFLKVSSKKKKTQPKLSALSTGSNALMTDSIHQRPGAQVGPFHPAAPSSLCHICMPLVASVMNGSSSDIPLPSAVLFPSPPSWIQVCKFTMPRSSLLAISGSLVFALPLDL

Target ORF information:

RefSeq Version	XM_006512739.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 5 (Map3k5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512739.3

ORF Insert Sequence:

ATGGGCACCG AAGCCGGCGA GGGCATCACT TTCTCCGTGC CGCCCTTTGC GTCCGTGGGC 
TTTTGCACCA TCCCGGAGGG CGGCAGCTGC CGGAGGGGAG GAGGAGCGGC GACGGCGGCG 
GAGGGAGAGC CGAGCCTGCA GCCCTTGCTT GTGCCGCCGC CGCCGCCACC GCCGGGCAGT 
TTCTGGAACG TGGAGAGCGC GGCGGCTCCC GGCACCAGCT GCCCTACCAC CGCCCCCGGG 
AGCAGCGCCA CCCGAGGCCG GGGCAACTCT GGCAGCGGGG GCGGCCGGCG TACCACCGTG 
GCTTATGTGA TCAATGAGGC GAGCCAGGGG CAGCTGGTGG TGGCGGAGAG CGAGGCCCTG 
CAGAGCCTGC GGGAGGCGTG CGAGGCGGTG GGCGCCACCC TGGAGACCCT GCATTTCGGG 
AAGCTGGACT TCGGGGAAAC CGCCGTGCTG GACCGTTTTT ACAACGCAGA TATTGCTGTG 
GTGGAGATGA GCGACGCCTT CCGGCAGCCT TCCTTGTTTT ACCATCTTGG AGTGCGAGAA 
AGCTTCAGTA TGGCCAACAA CATCATCCTC TACTGCGATA CTAATTCCGA TTCACTCCAG 
TCCCTGAAGG AAATTATTTG CCAGAAGAAT ACTGTGTGCA CCGGGAACTA CACCTTCATC 
CCCTACATGG TGACACCACA CAACAAGGTC TACTGCTGTG ACAGCAGCTT CATGAAGGGC 
CTGACTGAGC TCATGCAGCC TAACTTCGAG CTCCTGCTGG GGCCCATCTG CTTACCCCTC 
GTGGACCGTT TCGTTCAGCT TTTGAAGGTG GCGCAAGCGA GTTCCAGCCA GTACTTCCGG 
GAATCCATAC TCAGCGATAT CAGGAAAGCA CGGAACCTGT ACACTGGTAA AGAATTGGCA 
GCTGAATTGG CGAGGATCCG GCAACGGGTA GACAATATTG AAGTTTTGAC AGCAGACATT 
GTTATCAACC TGTTGCTTTC CTACAGAGAC ATCCAGGACT ATGACTCCAT AGTGAAGCTG 
GTAGAGACGT TAGAAAAACT TCCAACCTTC GACTTGGCCT CGCACCACCA CGTGAAGTTT 
CATTACGCAT TTGCACTGAA CAGGAGAAAT CTCCCTGGAG ACAGAGCAAA AGCTCTTGAC 
ATCATGATCC CCATGGTACA AAGTGAAGAG CAAGTTGCTT CGGATATGTA TTGTCTCGTT 
GGCCGAATCT ACAAAGATAT GTTTTTGGAT TCAAATTTCA CTGACACGGA AAGCAGGGAC 
CATGGAGCAT CTTGGTTCAA AAAGGCATTT GAGTCTGAGC CAACACTACA GTCAGGAATT 
AATTATGCAG TTCTCTTGCT GGCGGCTGGG CACCAGTTTG AATCTTCTTT TGAGCTTCGG 
AAAGTTGGAG TGAAGCTGAG TAGCCTTCTG GGTAAAAAGG GGAACTTGGA AAAACTCCAG 
AGCTACTGGG AAGTCGGATT TTTTCTGGGA GCCAGTGTCC TGGCCAACGA CCACCTCAGG 
GTCATTCAGG CATCCGAGAA GCTGTTCAGA CTGAAGACAC CAGCGTGGTA CCTCAAGTCT 
ATCGTGGAGA CCATTCTGAT ATATAAACAC TTTGTGAAAC TGACCACAGA GCAGCCCTCG 
GCTAAGCAGG AACTCGTGGA CTTCTGGATG GATTTCCTGG TCGAGGCCAC AAAAACAGAT 
GTTACTGTTG TCAGGTTCCC AGTATTAATA TTAGAGCCAA CCAAAATCTA TCAGCCTTCT 
TACCTGTCTA TCAATAATGA AGTTGAGGAG AAGACAATAT CTATTTGGCA TGTTCTCCCT 
GATGACAAGA AAGGTATACA CGAGTGGAAC TTCGGTGCTT CCTCTGTCAG GGGAGTGAGC 
ATCTCTAAGT TTGAAGAACG ATGCTGTTTC CTTTATGTGC TTCACAATTC TGATGACTTC 
CAAATCTACT TCTGCACAGA ACTTCACTGT AAAAGGTTTT TTGAAATGGT GAATACCATC 
ACGGAAGAGA AGGGGAGAGG CGCGGAGGAC GGAGACTGTG AGGGTGACTC CCTGGAGTAT 
GACTACGAAT ACGATGAAAA TGGAGACAGA GTTGTGTTAG GGAAGGGCAC TTATGGGATC 
GTCTATGCAG GACGAGACCT GAGCAACCAG GTCAGAATTG CTATTAAGGA AATCCCAGAG 
AGAGACAGCA GATACTCTCA GCCCCTGCAT GAAGAAATCG CCCTGCACAA GCATCTGAAA 
CACAAAAACA TTGTCCAGTA TCTGGGCTCC TTCAGTGAGA ACGGCTTCAT CAAAATCTTC 
ATGGAGCAAG TCCCAGGAGG AAGCCTCTCT GCTCTCCTTC GTTCAAAATG GGGCCCATTA 
AAGGACAACG AACAGACGAT TGGCTTCTAT ACGAAGCAGA TACTGGAAGG ATTAAAATAC 
CTTCACGACA ATCAGATAGT CCACCGGGAT ATAAAGGGTG ACAATGTATT GATTAATACC 
TACAGTGGTG TGCTCAAGAT CTCTGACTTC GGGACATCCA AGAGGCTTGC TGGCATAAAC 
CCATGTACCG AAACCTTTAC AGGTACCCTT CAGTATATGG CGCCAGAAAT AATCGATAAA 
GGACCACGAG GCTATGGAAA GGCCGCCGAC ATTTGGTCAC TGGGCTGCAC AATCATCGAA 
ATGGCTACTG GAAAACCACC ATTTTATGAA CTAGGAGAGC CACAGGCAGC CATGTTCAAG 
GTGGGGATGT TTAAGGTCCA CCCGGAGATC CCAGAGTCCA TGTCGGCGGA GGCCAAGGCG 
TTCATACTGA AGTGTTTTGA ACCAGACCCT GACAAGAGAG CCTGTGCTAA TGACTTGCTT 
ATTGATGAGT TCTTAAAAGT GTCCAGCAAA AAGAAAAAGA CACAACCCAA ACTTTCAGCT 
CTCTCGACTG GATCGAATGC CTTAATGACA GACAGCATCC ATCAGAGGCC AGGAGCCCAG 
GTGGGTCCTT TCCACCCAGC CGCCCCCTCT AGTCTATGTC ACATATGCAT GCCTTTGGTG 
GCTTCTGTCA TGAACGGGTC CTCTTCAGAC ATCCCCTTGC CTAGCGCTGT GCTTTTTCCC 
TCTCCGCCAT CATGGATTCA GGTCTGCAAG TTCACTATGC CCAGGTCTTC TCTGCTTGCT 
ATTTCTGGCA GTCTAGTATT TGCCCTTCCA CTTGACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map3k5 ( XM_006512739.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512802.1 Mus musculus mitogen-activated protein kinase kinase kinase 5 (Map3k5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43874
Clone ID Related Accession (Same CDS sequence)	XM_006512739.3 , XM_006512739.4
Accession Version	XM_006512739.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512739.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCG AAGCCGGCGA GGGCATCACT TTCTCCGTGC CGCCCTTTGC GTCCGTGGGC 
TTTTGCACCA TCCCGGAGGG CGGCAGCTGC CGGAGGGGAG GAGGAGCGGC GACGGCGGCG 
GAGGGAGAGC CGAGCCTGCA GCCCTTGCTT GTGCCGCCGC CGCCGCCACC GCCGGGCAGT 
TTCTGGAACG TGGAGAGCGC GGCGGCTCCC GGCACCAGCT GCCCTACCAC CGCCCCCGGG 
AGCAGCGCCA CCCGAGGCCG GGGCAACTCT GGCAGCGGGG GCGGCCGGCG TACCACCGTG 
GCTTATGTGA TCAATGAGGC GAGCCAGGGG CAGCTGGTGG TGGCGGAGAG CGAGGCCCTG 
CAGAGCCTGC GGGAGGCGTG CGAGGCGGTG GGCGCCACCC TGGAGACCCT GCATTTCGGG 
AAGCTGGACT TCGGGGAAAC CGCCGTGCTG GACCGTTTTT ACAACGCAGA TATTGCTGTG 
GTGGAGATGA GCGACGCCTT CCGGCAGCCT TCCTTGTTTT ACCATCTTGG AGTGCGAGAA 
AGCTTCAGTA TGGCCAACAA CATCATCCTC TACTGCGATA CTAATTCCGA TTCACTCCAG 
TCCCTGAAGG AAATTATTTG CCAGAAGAAT ACTGTGTGCA CCGGGAACTA CACCTTCATC 
CCCTACATGG TGACACCACA CAACAAGGTC TACTGCTGTG ACAGCAGCTT CATGAAGGGC 
CTGACTGAGC TCATGCAGCC TAACTTCGAG CTCCTGCTGG GGCCCATCTG CTTACCCCTC 
GTGGACCGTT TCGTTCAGCT TTTGAAGGTG GCGCAAGCGA GTTCCAGCCA GTACTTCCGG 
GAATCCATAC TCAGCGATAT CAGGAAAGCA CGGAACCTGT ACACTGGTAA AGAATTGGCA 
GCTGAATTGG CGAGGATCCG GCAACGGGTA GACAATATTG AAGTTTTGAC AGCAGACATT 
GTTATCAACC TGTTGCTTTC CTACAGAGAC ATCCAGGACT ATGACTCCAT AGTGAAGCTG 
GTAGAGACGT TAGAAAAACT TCCAACCTTC GACTTGGCCT CGCACCACCA CGTGAAGTTT 
CATTACGCAT TTGCACTGAA CAGGAGAAAT CTCCCTGGAG ACAGAGCAAA AGCTCTTGAC 
ATCATGATCC CCATGGTACA AAGTGAAGAG CAAGTTGCTT CGGATATGTA TTGTCTCGTT 
GGCCGAATCT ACAAAGATAT GTTTTTGGAT TCAAATTTCA CTGACACGGA AAGCAGGGAC 
CATGGAGCAT CTTGGTTCAA AAAGGCATTT GAGTCTGAGC CAACACTACA GTCAGGAATT 
AATTATGCAG TTCTCTTGCT GGCGGCTGGG CACCAGTTTG AATCTTCTTT TGAGCTTCGG 
AAAGTTGGAG TGAAGCTGAG TAGCCTTCTG GGTAAAAAGG GGAACTTGGA AAAACTCCAG 
AGCTACTGGG AAGTCGGATT TTTTCTGGGA GCCAGTGTCC TGGCCAACGA CCACCTCAGG 
GTCATTCAGG CATCCGAGAA GCTGTTCAGA CTGAAGACAC CAGCGTGGTA CCTCAAGTCT 
ATCGTGGAGA CCATTCTGAT ATATAAACAC TTTGTGAAAC TGACCACAGA GCAGCCCTCG 
GCTAAGCAGG AACTCGTGGA CTTCTGGATG GATTTCCTGG TCGAGGCCAC AAAAACAGAT 
GTTACTGTTG TCAGGTTCCC AGTATTAATA TTAGAGCCAA CCAAAATCTA TCAGCCTTCT 
TACCTGTCTA TCAATAATGA AGTTGAGGAG AAGACAATAT CTATTTGGCA TGTTCTCCCT 
GATGACAAGA AAGGTATACA CGAGTGGAAC TTCGGTGCTT CCTCTGTCAG GGGAGTGAGC 
ATCTCTAAGT TTGAAGAACG ATGCTGTTTC CTTTATGTGC TTCACAATTC TGATGACTTC 
CAAATCTACT TCTGCACAGA ACTTCACTGT AAAAGGTTTT TTGAAATGGT GAATACCATC 
ACGGAAGAGA AGGGGAGAGG CGCGGAGGAC GGAGACTGTG AGGGTGACTC CCTGGAGTAT 
GACTACGAAT ACGATGAAAA TGGAGACAGA GTTGTGTTAG GGAAGGGCAC TTATGGGATC 
GTCTATGCAG GACGAGACCT GAGCAACCAG GTCAGAATTG CTATTAAGGA AATCCCAGAG 
AGAGACAGCA GATACTCTCA GCCCCTGCAT GAAGAAATCG CCCTGCACAA GCATCTGAAA 
CACAAAAACA TTGTCCAGTA TCTGGGCTCC TTCAGTGAGA ACGGCTTCAT CAAAATCTTC 
ATGGAGCAAG TCCCAGGAGG AAGCCTCTCT GCTCTCCTTC GTTCAAAATG GGGCCCATTA 
AAGGACAACG AACAGACGAT TGGCTTCTAT ACGAAGCAGA TACTGGAAGG ATTAAAATAC 
CTTCACGACA ATCAGATAGT CCACCGGGAT ATAAAGGGTG ACAATGTATT GATTAATACC 
TACAGTGGTG TGCTCAAGAT CTCTGACTTC GGGACATCCA AGAGGCTTGC TGGCATAAAC 
CCATGTACCG AAACCTTTAC AGGTACCCTT CAGTATATGG CGCCAGAAAT AATCGATAAA 
GGACCACGAG GCTATGGAAA GGCCGCCGAC ATTTGGTCAC TGGGCTGCAC AATCATCGAA 
ATGGCTACTG GAAAACCACC ATTTTATGAA CTAGGAGAGC CACAGGCAGC CATGTTCAAG 
GTGGGGATGT TTAAGGTCCA CCCGGAGATC CCAGAGTCCA TGTCGGCGGA GGCCAAGGCG 
TTCATACTGA AGTGTTTTGA ACCAGACCCT GACAAGAGAG CCTGTGCTAA TGACTTGCTT 
ATTGATGAGT TCTTAAAAGT GTCCAGCAAA AAGAAAAAGA CACAACCCAA ACTTTCAGCT 
CTCTCGACTG GATCGAATGC CTTAATGACA GACAGCATCC ATCAGAGGCC AGGAGCCCAG 
GTGGGTCCTT TCCACCCAGC CGCCCCCTCT AGTCTATGTC ACATATGCAT GCCTTTGGTG 
GCTTCTGTCA TGAACGGGTC CTCTTCAGAC ATCCCCTTGC CTAGCGCTGT GCTTTTTCCC 
TCTCCGCCAT CATGGATTCA GGTCTGCAAG TTCACTATGC CCAGGTCTTC TCTGCTTGCT 
ATTTCTGGCA GTCTAGTATT TGCCCTTCCA CTTGACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512802.1
CDS	506..3667
Translation	MGTEAGEGITFSVPPFASVGFCTIPEGGSCRRGGGAATAAEGEPSLQPLLVPPPPPPPGSFWNVESAAAPGTSCPTTAPGSSATRGRGNSGSGGGRRTTVAYVINEASQGQLVVAESEALQSLREACEAVGATLETLHFGKLDFGETAVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTVCTGNYTFIPYMVTPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFVQLLKVAQASSSQYFRESILSDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEEQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHLRVIQASEKLFRLKTPAWYLKSIVETILIYKHFVKLTTEQPSAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFGASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKRFFEMVNTITEEKGRGAEDGDCEGDSLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLIDEFLKVSSKKKKTQPKLSALSTGSNALMTDSIHQRPGAQVGPFHPAAPSSLCHICMPLVASVMNGSSSDIPLPSAVLFPSPPSWIQVCKFTMPRSSLLAISGSLVFALPLDL

Target ORF information:

RefSeq Version	XM_006512739.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 5 (Map3k5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512739.4

ORF Insert Sequence:

ATGGGCACCG AAGCCGGCGA GGGCATCACT TTCTCCGTGC CGCCCTTTGC GTCCGTGGGC 
TTTTGCACCA TCCCGGAGGG CGGCAGCTGC CGGAGGGGAG GAGGAGCGGC GACGGCGGCG 
GAGGGAGAGC CGAGCCTGCA GCCCTTGCTT GTGCCGCCGC CGCCGCCACC GCCGGGCAGT 
TTCTGGAACG TGGAGAGCGC GGCGGCTCCC GGCACCAGCT GCCCTACCAC CGCCCCCGGG 
AGCAGCGCCA CCCGAGGCCG GGGCAACTCT GGCAGCGGGG GCGGCCGGCG TACCACCGTG 
GCTTATGTGA TCAATGAGGC GAGCCAGGGG CAGCTGGTGG TGGCGGAGAG CGAGGCCCTG 
CAGAGCCTGC GGGAGGCGTG CGAGGCGGTG GGCGCCACCC TGGAGACCCT GCATTTCGGG 
AAGCTGGACT TCGGGGAAAC CGCCGTGCTG GACCGTTTTT ACAACGCAGA TATTGCTGTG 
GTGGAGATGA GCGACGCCTT CCGGCAGCCT TCCTTGTTTT ACCATCTTGG AGTGCGAGAA 
AGCTTCAGTA TGGCCAACAA CATCATCCTC TACTGCGATA CTAATTCCGA TTCACTCCAG 
TCCCTGAAGG AAATTATTTG CCAGAAGAAT ACTGTGTGCA CCGGGAACTA CACCTTCATC 
CCCTACATGG TGACACCACA CAACAAGGTC TACTGCTGTG ACAGCAGCTT CATGAAGGGC 
CTGACTGAGC TCATGCAGCC TAACTTCGAG CTCCTGCTGG GGCCCATCTG CTTACCCCTC 
GTGGACCGTT TCGTTCAGCT TTTGAAGGTG GCGCAAGCGA GTTCCAGCCA GTACTTCCGG 
GAATCCATAC TCAGCGATAT CAGGAAAGCA CGGAACCTGT ACACTGGTAA AGAATTGGCA 
GCTGAATTGG CGAGGATCCG GCAACGGGTA GACAATATTG AAGTTTTGAC AGCAGACATT 
GTTATCAACC TGTTGCTTTC CTACAGAGAC ATCCAGGACT ATGACTCCAT AGTGAAGCTG 
GTAGAGACGT TAGAAAAACT TCCAACCTTC GACTTGGCCT CGCACCACCA CGTGAAGTTT 
CATTACGCAT TTGCACTGAA CAGGAGAAAT CTCCCTGGAG ACAGAGCAAA AGCTCTTGAC 
ATCATGATCC CCATGGTACA AAGTGAAGAG CAAGTTGCTT CGGATATGTA TTGTCTCGTT 
GGCCGAATCT ACAAAGATAT GTTTTTGGAT TCAAATTTCA CTGACACGGA AAGCAGGGAC 
CATGGAGCAT CTTGGTTCAA AAAGGCATTT GAGTCTGAGC CAACACTACA GTCAGGAATT 
AATTATGCAG TTCTCTTGCT GGCGGCTGGG CACCAGTTTG AATCTTCTTT TGAGCTTCGG 
AAAGTTGGAG TGAAGCTGAG TAGCCTTCTG GGTAAAAAGG GGAACTTGGA AAAACTCCAG 
AGCTACTGGG AAGTCGGATT TTTTCTGGGA GCCAGTGTCC TGGCCAACGA CCACCTCAGG 
GTCATTCAGG CATCCGAGAA GCTGTTCAGA CTGAAGACAC CAGCGTGGTA CCTCAAGTCT 
ATCGTGGAGA CCATTCTGAT ATATAAACAC TTTGTGAAAC TGACCACAGA GCAGCCCTCG 
GCTAAGCAGG AACTCGTGGA CTTCTGGATG GATTTCCTGG TCGAGGCCAC AAAAACAGAT 
GTTACTGTTG TCAGGTTCCC AGTATTAATA TTAGAGCCAA CCAAAATCTA TCAGCCTTCT 
TACCTGTCTA TCAATAATGA AGTTGAGGAG AAGACAATAT CTATTTGGCA TGTTCTCCCT 
GATGACAAGA AAGGTATACA CGAGTGGAAC TTCGGTGCTT CCTCTGTCAG GGGAGTGAGC 
ATCTCTAAGT TTGAAGAACG ATGCTGTTTC CTTTATGTGC TTCACAATTC TGATGACTTC 
CAAATCTACT TCTGCACAGA ACTTCACTGT AAAAGGTTTT TTGAAATGGT GAATACCATC 
ACGGAAGAGA AGGGGAGAGG CGCGGAGGAC GGAGACTGTG AGGGTGACTC CCTGGAGTAT 
GACTACGAAT ACGATGAAAA TGGAGACAGA GTTGTGTTAG GGAAGGGCAC TTATGGGATC 
GTCTATGCAG GACGAGACCT GAGCAACCAG GTCAGAATTG CTATTAAGGA AATCCCAGAG 
AGAGACAGCA GATACTCTCA GCCCCTGCAT GAAGAAATCG CCCTGCACAA GCATCTGAAA 
CACAAAAACA TTGTCCAGTA TCTGGGCTCC TTCAGTGAGA ACGGCTTCAT CAAAATCTTC 
ATGGAGCAAG TCCCAGGAGG AAGCCTCTCT GCTCTCCTTC GTTCAAAATG GGGCCCATTA 
AAGGACAACG AACAGACGAT TGGCTTCTAT ACGAAGCAGA TACTGGAAGG ATTAAAATAC 
CTTCACGACA ATCAGATAGT CCACCGGGAT ATAAAGGGTG ACAATGTATT GATTAATACC 
TACAGTGGTG TGCTCAAGAT CTCTGACTTC GGGACATCCA AGAGGCTTGC TGGCATAAAC 
CCATGTACCG AAACCTTTAC AGGTACCCTT CAGTATATGG CGCCAGAAAT AATCGATAAA 
GGACCACGAG GCTATGGAAA GGCCGCCGAC ATTTGGTCAC TGGGCTGCAC AATCATCGAA 
ATGGCTACTG GAAAACCACC ATTTTATGAA CTAGGAGAGC CACAGGCAGC CATGTTCAAG 
GTGGGGATGT TTAAGGTCCA CCCGGAGATC CCAGAGTCCA TGTCGGCGGA GGCCAAGGCG 
TTCATACTGA AGTGTTTTGA ACCAGACCCT GACAAGAGAG CCTGTGCTAA TGACTTGCTT 
ATTGATGAGT TCTTAAAAGT GTCCAGCAAA AAGAAAAAGA CACAACCCAA ACTTTCAGCT 
CTCTCGACTG GATCGAATGC CTTAATGACA GACAGCATCC ATCAGAGGCC AGGAGCCCAG 
GTGGGTCCTT TCCACCCAGC CGCCCCCTCT AGTCTATGTC ACATATGCAT GCCTTTGGTG 
GCTTCTGTCA TGAACGGGTC CTCTTCAGAC ATCCCCTTGC CTAGCGCTGT GCTTTTTCCC 
TCTCCGCCAT CATGGATTCA GGTCTGCAAG TTCACTATGC CCAGGTCTTC TCTGCTTGCT 
ATTTCTGGCA GTCTAGTATT TGCCCTTCCA CTTGACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR-23b promotes cutaneous wound healing through inhibition of the inflammatory responses by targeting ASK1.
Acta biochimica et biophysica Sinica50(11)1104-1113(2018 Nov)
Li H,Han X,Zuo K,Li L,Liu J,Yuan X,Shen Y,Shao M,Pang D,Chu Y,Zhao B

β-TrCP-dependent degradation of ASK1 suppresses the induction of the apoptotic response by oxidative stress.
Biochimica et biophysica acta. General subjects1862(10)2271-2280(2018 10)
Cheng R,Takeda K,Naguro I,Hatta T,Iemura SI,Natsume T,Ichijo H,Hattori K

Regulator of G Protein Signaling 6 Facilities Cardiac Hypertrophy by Activating Apoptosis Signal-Regulating Kinase 1-P38/c-JUN N-Terminal Kinase 1/2 Signaling.
Journal of the American Heart Association7(19)e009179(2018 10)
Huang Z,Shu J,Jiang W,Jiang M,Lu Y,Dai H,Xu N,Yuan H,Cai J

ASK1 contributes to fibrosis and dysfunction in models of kidney disease.
The Journal of clinical investigation128(10)4485-4500(2018 10)
Liles JT,Corkey BK,Notte GT,Budas GR,Lansdon EB,Hinojosa-Kirschenbaum F,Badal SS,Lee M,Schultz BE,Wise S,Pendem S,Graupe M,Castonguay L,Koch KA,Wong MH,Papalia GA,French DM,Sullivan T,Huntzicker EG,Ma FY,Nikolic-Paterson DJ,Altuhaifi T,Yang H,Fogo AB,Breckenridge DG

Apoptosis Signal-regulating Kinase 1 Silencing on Astroglial Inflammasomes in an Experimental Model of Ischemic Stroke.
Neuroscience390218-230(2018 10)
Cheon SY,Kim EJ,Kim SY,Kim JM,Kam EH,Park JK,Koo BN

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

Map3k5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM