otol1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	otol1a
Entrez Gene ID	553759
Full Name	otolin 1a
Synonyms	Gm414,otol1,si:dkey-119f1.2
General protein information	Preferred Names otolin 1a Names otolin-1-A Otolin1.1 otolin 1 zOtolin1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001099741.1	NP_001093211.1	otolin-1-A precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Otol1	XP_006232550.1
Xenopus tropicalis (tropical clawed frog)	LOC100495841	XP_002933297.2
Danio rerio (zebrafish)	otol1a	NP_001093211.1
Canis lupus familiaris (dog)	OTOL1	XP_003640182.1
Bos taurus (cattle)	OTOL1	NP_001179533.1
Gallus gallus (chicken)	OTOL1	XP_426716.3
Danio rerio (zebrafish)	LOC572364	XP_701165.5
Homo sapiens (human)	OTOL1	NP_001073909.1
Pan troglodytes (chimpanzee)	OTOL1	XP_526369.3
Macaca mulatta (Rhesus monkey)	LOC100426070	XP_002802984.1
Mus musculus (house mouse)	Otol1	NP_001018041.2

Gene Ontology
Bibliography

Related articles in PubMed

Effect of calcium ions on structure and stability of the C1q-like domain of otolin-1 from human and zebrafish.
Hołubowicz R, Wojtas M, Taube M, Kozak M, Ożyhar A, Dobryszycki P
The FEBS journal284(24)4278-4297(2017 12)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Characterization of C1q in teleosts: insight into the molecular and functional evolution of C1q family and classical pathway.
Hu YL, Pan XM, Xiang LX, Shao JZ
The Journal of biological chemistry285(37)28777-86(2010 Sep)

Bioinformatic identification of genes encoding C1q-domain-containing proteins in zebrafish.
Mei J, Gui J
Journal of genetics and genomics = Yi chuan xue bao35(1)17-24(2008 Jan)

Otolith matrix proteins OMP-1 and Otolin-1 are necessary for normal otolith growth and their correct anchoring onto the sensory maculae.
Murayama E, Herbomel P, Kawakami A, Takeda H, Nagasawa H
Mechanisms of development122(6)791-803(2005 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following otol1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the otol1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00182	NM_001099741.1 Latest version!	Danio rerio otolin 1a (otol1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

otol1a ( NM_001099741.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001093211.1 Danio rerio otolin 1a (otol1a), mRNA.

Price & Availability↑

CloneID	ODa00182
Clone ID Related Accession (Same CDS sequence)	NM_001099741.1
Accession Version	NM_001099741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551024000000
Organism	Danio rerio(zebrafish)
Product	otolin-1-A precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX927289.13. ##Evidence-Data-START## Transcript exon combination :: AB124554.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGCCCAATA TTCTCCACCC GTTCATCATA ATCATGACCC TGCTGGTGGT AGCAACAGGG 
AACCAAGCAT CAATAGACAA GACCACACAG TGGCCCCGAA TGAAGCCCAC TAAGAAGCCT 
CCGCCTCGGG ATGAAGGTCC GTCAAAATTG GGGTCCATCT CCACCACTGT GAGTCCAACA 
GCAATTGGCA TCACTGAAGA AGTTACAGAC GCCATGATGG ATGCATACAC GATTACGTCC 
ACCGGTAGTA CCACTTTTTC CAGTGATACT TATTCTGCAG ACTACCACAC GGAGGCCATG 
GTTCCTCCTG GGGTGGGTCC TGGAAACTAC ACGCTGGACT ACAACGAGTG CTTCTTCAAC 
TTTTGTGAAT GTTGTCCACC CGAGAGAGGT CCTCCGGGGC CGGTTGGAGA AAAGGGATTG 
CCGGGTATTC CAGGGGGAAA GGGGGAGATG GGACCTCCGG GACCTCCAGG TCAAGAAGGA 
CTCACTGGTG CTCCAGGAAC GCATGGAGTA AAAGGAGAGA AAGGCGATAC TGGAGCGAGC 
GGGCTACCTG GGATTCCTGG AGTTACTGGG AAACAAGGAG AGAAAGGTGA ATCAGGCCCT 
AAAGGAGACA AAGGAGATAC CGGTTTCCCC GGGCTGAAAG GAGACCCAGG GGAGCGCGGA 
GAGCCTGGCT GGAATGGAAC AAAGGGAGGG ATGGGTGAGC CTGGCAAACA GGGATTGACT 
GGTCCTCCTG GACCAGATGG CATCAAGGGC GAGAAGGGCG ACAAAGGAGA CTGTCCATTT 
GGAGAGAAAG GCCAGAAAGG CAGTATAGGG GAACCAGGGC CTCAAGGGCC CAAAGGAGAC 
CCGGGTGTGC CAGGAACAAA TGGAACAGAC GGGCTTCCTG GATCTAAAGG GCCAAAAGGA 
GATCCGGGAC CTCTAAGCAA GCAAGGAGAG CCTGGTCCAC CAGGCCCACA AGGACCACCA 
GGACAAAGGG GGATGCCGGG GATGAAAGGC ACAAGAGGTT TAAAAGGAGC ACGAGGAATT 
CGGGGATTCA AGGGCTTTAA AGGCGAACCT GCCGTTCAGA AGCGCTCTGC ATTCAGTGTT 
GGTCTTTTCC CCAGCCGATC GTTCCCGCCA CCAGGACTCC CCATCAGGTT CGACAAAATC 
ATTTACAATG AGGAGGCTCA CTGGGACCCC AATGCAAGTA AATTCAACTG CACTCACGGA 
GGAGTTTACG TCTTTTCATA CTACATAACT GTACGTAATC GTCCCCTGCG TGCAGCCCTG 
GTGGTGAACG GCATTCGGAA ACTGCGAACG CGTGACTCTC TTTACGGCCA GGACATTGAC 
CAGGCCTCCA ACATGGCCGT GCTCCGTCTG TCATCCGGAG ACCAGGTTTG GCTGGAGACT 
CTGCGGGACT GGAACGGTGT GTACTCCAGC AGTGAGGACG ACAGCACGTT CTCTGGATTC 
TTGCTCTATG CTGATGCCAC CAAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093211.1
CDS	51..1520
Translation	MPNILHPFIIIMTLLVVATGNQASIDKTTQWPRMKPTKKPPPRDEGPSKLGSISTTVSPTAIGITEEVTDAMMDAYTITSTGSTTFSSDTYSADYHTEAMVPPGVGPGNYTLDYNECFFNFCECCPPERGPPGPVGEKGLPGIPGGKGEMGPPGPPGQEGLTGAPGTHGVKGEKGDTGASGLPGIPGVTGKQGEKGESGPKGDKGDTGFPGLKGDPGERGEPGWNGTKGGMGEPGKQGLTGPPGPDGIKGEKGDKGDCPFGEKGQKGSIGEPGPQGPKGDPGVPGTNGTDGLPGSKGPKGDPGPLSKQGEPGPPGPQGPPGQRGMPGMKGTRGLKGARGIRGFKGFKGEPAVQKRSAFSVGLFPSRSFPPPGLPIRFDKIIYNEEAHWDPNASKFNCTHGGVYVFSYYITVRNRPLRAALVVNGIRKLRTRDSLYGQDIDQASNMAVLRLSSGDQVWLETLRDWNGVYSSSEDDSTFSGFLLYADATKD

Target ORF information:

RefSeq Version	NM_001099741.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio otolin 1a (otol1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099741.1

ORF Insert Sequence:

ATGCCCAATA TTCTCCACCC GTTCATCATA ATCATGACCC TGCTGGTGGT AGCAACAGGG 
AACCAAGCAT CAATAGACAA GACCACACAG TGGCCCCGAA TGAAGCCCAC TAAGAAGCCT 
CCGCCTCGGG ATGAAGGTCC GTCAAAATTG GGGTCCATCT CCACCACTGT GAGTCCAACA 
GCAATTGGCA TCACTGAAGA AGTTACAGAC GCCATGATGG ATGCATACAC GATTACGTCC 
ACCGGTAGTA CCACTTTTTC CAGTGATACT TATTCTGCAG ACTACCACAC GGAGGCCATG 
GTTCCTCCTG GGGTGGGTCC TGGAAACTAC ACGCTGGACT ACAACGAGTG CTTCTTCAAC 
TTTTGTGAAT GTTGTCCACC CGAGAGAGGT CCTCCGGGGC CGGTTGGAGA AAAGGGATTG 
CCGGGTATTC CAGGGGGAAA GGGGGAGATG GGACCTCCGG GACCTCCAGG TCAAGAAGGA 
CTCACTGGTG CTCCAGGAAC GCATGGAGTA AAAGGAGAGA AAGGCGATAC TGGAGCGAGC 
GGGCTACCTG GGATTCCTGG AGTTACTGGG AAACAAGGAG AGAAAGGTGA ATCAGGCCCT 
AAAGGAGACA AAGGAGATAC CGGTTTCCCC GGGCTGAAAG GAGACCCAGG GGAGCGCGGA 
GAGCCTGGCT GGAATGGAAC AAAGGGAGGG ATGGGTGAGC CTGGCAAACA GGGATTGACT 
GGTCCTCCTG GACCAGATGG CATCAAGGGC GAGAAGGGCG ACAAAGGAGA CTGTCCATTT 
GGAGAGAAAG GCCAGAAAGG CAGTATAGGG GAACCAGGGC CTCAAGGGCC CAAAGGAGAC 
CCGGGTGTGC CAGGAACAAA TGGAACAGAC GGGCTTCCTG GATCTAAAGG GCCAAAAGGA 
GATCCGGGAC CTCTAAGCAA GCAAGGAGAG CCTGGTCCAC CAGGCCCACA AGGACCACCA 
GGACAAAGGG GGATGCCGGG GATGAAAGGC ACAAGAGGTT TAAAAGGAGC ACGAGGAATT 
CGGGGATTCA AGGGCTTTAA AGGCGAACCT GCCGTTCAGA AGCGCTCTGC ATTCAGTGTT 
GGTCTTTTCC CCAGCCGATC GTTCCCGCCA CCAGGACTCC CCATCAGGTT CGACAAAATC 
ATTTACAATG AGGAGGCTCA CTGGGACCCC AATGCAAGTA AATTCAACTG CACTCACGGA 
GGAGTTTACG TCTTTTCATA CTACATAACT GTACGTAATC GTCCCCTGCG TGCAGCCCTG 
GTGGTGAACG GCATTCGGAA ACTGCGAACG CGTGACTCTC TTTACGGCCA GGACATTGAC 
CAGGCCTCCA ACATGGCCGT GCTCCGTCTG TCATCCGGAG ACCAGGTTTG GCTGGAGACT 
CTGCGGGACT GGAACGGTGT GTACTCCAGC AGTGAGGACG ACAGCACGTT CTCTGGATTC 
TTGCTCTATG CTGATGCCAC CAAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of calcium ions on structure and stability of the C1q-like domain of otolin-1 from human and zebrafish.
The FEBS journal284(24)4278-4297(2017 12)
Hołubowicz R,Wojtas M,Taube M,Kozak M,Ożyhar A,Dobryszycki P

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Characterization of C1q in teleosts: insight into the molecular and functional evolution of C1q family and classical pathway.
The Journal of biological chemistry285(37)28777-86(2010 Sep)
Hu YL,Pan XM,Xiang LX,Shao JZ

Bioinformatic identification of genes encoding C1q-domain-containing proteins in zebrafish.
Journal of genetics and genomics = Yi chuan xue bao35(1)17-24(2008 Jan)
Mei J,Gui J

Otolith matrix proteins OMP-1 and Otolin-1 are necessary for normal otolith growth and their correct anchoring onto the sensory maculae.
Mechanisms of development122(6)791-803(2005 Jun)
Murayama E,Herbomel P,Kawakami A,Takeda H,Nagasawa H