nucks1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nucks1a
Entrez Gene ID	447862
Full Name	nuclear casein kinase and cyclin-dependent kinase substrate 1a
Synonyms	fc07e02,nucks1,wu:fb16a10,wu:fb16h08,wu:fb36b01,wu:fb82a01,wu:fc07e02,wu:fe37f10,wu:gcd26e12,zgc:86878
General protein information	Preferred Names nuclear casein kinase and cyclin-dependent kinase substrate 1a Names nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 nuclear casein kinase and cyclin-dependent kinase substrate 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001004601.2	NP_001004601.1	nuclear ubiquitous casein and cyclin-dependent kinase substrate 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nucks1	NP_780503.2
Gallus gallus (chicken)	NUCKS1	NP_998746.1
Rattus norvegicus (Norway rat)	Nucks1	NP_073636.1
Pan troglodytes (chimpanzee)	NUCKS1	XP_514141.3
Xenopus tropicalis (tropical clawed frog)	nucks1	NP_989187.1
Canis lupus familiaris (dog)	NUCKS1	XP_005640853.1
Rattus norvegicus (Norway rat)	LOC102546716	XP_006240768.1
Homo sapiens (human)	NUCKS1	NP_073568.2
Macaca mulatta (Rhesus monkey)	NUCKS1	XP_001092174.1
Bos taurus (cattle)	NUCKS1	NP_001039385.1
Danio rerio (zebrafish)	nucks1a	NP_001004601.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Identification and characterization of two putative nuclear localization signals (NLS) in the DNA-binding protein NUCKS.
Grundt K, Haga IV, Huitfeldt HS, Ostvold AC
Biochimica et biophysica acta1773(9)1398-406(2007 Sep)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

Characterisation of the NUCKS gene on human chromosome 1q32.1 and the presence of a homologous gene in different species.
Grundt K, Haga IV, Aleporou-Marinou V, Drosos Y, Wanvik B, Østvold AC
Biochemical and biophysical research communications323(3)796-801(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nucks1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nucks1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37113	NM_001004601.2 Latest version!	Danio rerio nuclear casein kinase and cyclin-dependent kinase substrate 1a (nucks1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nucks1a ( NM_001004601.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001004601.1 Danio rerio nuclear casein kinase and cyclin-dependent kinase substrate 1a (nucks1a), mRNA.

Price & Availability↑

CloneID	ODa37113
Clone ID Related Accession (Same CDS sequence)	NM_001004601.2
Accession Version	NM_001004601.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	nuclear ubiquitous casein and cyclin-dependent kinase substrate 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AI588726.1, CT705076.2, CT611690.2, CT641760.1, CT614653.2 and CD605188.1. On Jul 14, 2011 this sequence version replaced NM_001004601.1. ##Evidence-Data-START## Transcript exon combination :: BC081557.1, GW707715.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476732, SAMEA4476752 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGAC CAGTGAGGAA CAGAAAGGTG GTGAATTACT CCCAGTTTAA CGAATCTGAT 
GATGCAGATG AGGATTATGG AAGAAAGGAG AAGGAAACCA CCAAGAAGAT ACGTGCTCCA 
CCCCGTGAAA ACAAGCACAA GAAGTCTAAG AATTCGCAGG AAGAAAGCGA GGATTCAGAT 
GAAAAACTGA CCAAATCCAA AAATGATTCG GCAGATGACT TCGGTAGCGA TGAAGACAAC 
GACTTTGGAG AAGAGCAGGA GGAAGATGGA GGAAGCGACT ATGGTGATAA AAAGGCTAAA 
AAGGGAAAGA AGCCAAAGGT GGAGAAGCCT GGCAAAAAGT CACTGAAGAG GAAACGAGGT 
GGAGATGATA GTGATGATGA TAAAGAGGTG AGCAGGAAAC CGAGGCAGGT GAGACAGGCT 
GCGTCAAAGG CTGTGTCCAA ACAGAGAGAA ATCCTGCTGG GAGACGGAGG CAGTGAGGAT 
GAGGAGAAGG ATGAGGAAGA ACAGGCCTTC CTGGACCAAG AGTCAGGCAG TGATGAAGAC 
TTCATGGTTG ATGATGATGA TGACAGCGAT TATGGCCACT CCAAAAAGAA GGGCAAAAAA 
GTGGTGCCAA GAGGTGGAGG ACGGAGAGTG GAGAAGAAGG AGAAGAAATC CCCCAAACCC 
CGGCTAAAGG CTACAGTGAA CCCCAGCCCT ATGAAAGGCA AAGGTAAAGG GCGCCCGAGC 
GCAGCTAAAG CGCTGGAGAA ATCCTCGCCT AAAGATGAAG AGGAAGCCGA GAGTCCCGCT 
GAAGATGAAG AGGAAGACGA GGTGGAGAAG AAAGAGTCTC CTCCATCCAA AAAGACGAAG 
GATGAAGCCC CCGAAGACGA GGAAGAAGAT GAAGAAGAGG ACGGCTCAGA AGAGGAGGCT 
CCATCCGGGG AAGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004601.1
CDS	241..1158
Translation	MARPVRNRKVVNYSQFNESDDADEDYGRKEKETTKKIRAPPRENKHKKSKNSQEESEDSDEKLTKSKNDSADDFGSDEDNDFGEEQEEDGGSDYGDKKAKKGKKPKVEKPGKKSLKRKRGGDDSDDDKEVSRKPRQVRQAASKAVSKQREILLGDGGSEDEEKDEEEQAFLDQESGSDEDFMVDDDDDSDYGHSKKKGKKVVPRGGGRRVEKKEKKSPKPRLKATVNPSPMKGKGKGRPSAAKALEKSSPKDEEEAESPAEDEEEDEVEKKESPPSKKTKDEAPEDEEEDEEEDGSEEEAPSGED

Target ORF information:

RefSeq Version	NM_001004601.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear casein kinase and cyclin-dependent kinase substrate 1a (nucks1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004601.2

ORF Insert Sequence:

ATGGCAAGAC CAGTGAGGAA CAGAAAGGTG GTGAATTACT CCCAGTTTAA CGAATCTGAT 
GATGCAGATG AGGATTATGG AAGAAAGGAG AAGGAAACCA CCAAGAAGAT ACGTGCTCCA 
CCCCGTGAAA ACAAGCACAA GAAGTCTAAG AATTCGCAGG AAGAAAGCGA GGATTCAGAT 
GAAAAACTGA CCAAATCCAA AAATGATTCG GCAGATGACT TCGGTAGCGA TGAAGACAAC 
GACTTTGGAG AAGAGCAGGA GGAAGATGGA GGAAGCGACT ATGGTGATAA AAAGGCTAAA 
AAGGGAAAGA AGCCAAAGGT GGAGAAGCCT GGCAAAAAGT CACTGAAGAG GAAACGAGGT 
GGAGATGATA GTGATGATGA TAAAGAGGTG AGCAGGAAAC CGAGGCAGGT GAGACAGGCT 
GCGTCAAAGG CTGTGTCCAA ACAGAGAGAA ATCCTGCTGG GAGACGGAGG CAGTGAGGAT 
GAGGAGAAGG ATGAGGAAGA ACAGGCCTTC CTGGACCAAG AGTCAGGCAG TGATGAAGAC 
TTCATGGTTG ATGATGATGA TGACAGCGAT TATGGCCACT CCAAAAAGAA GGGCAAAAAA 
GTGGTGCCAA GAGGTGGAGG ACGGAGAGTG GAGAAGAAGG AGAAGAAATC CCCCAAACCC 
CGGCTAAAGG CTACAGTGAA CCCCAGCCCT ATGAAAGGCA AAGGTAAAGG GCGCCCGAGC 
GCAGCTAAAG CGCTGGAGAA ATCCTCGCCT AAAGATGAAG AGGAAGCCGA GAGTCCCGCT 
GAAGATGAAG AGGAAGACGA GGTGGAGAAG AAAGAGTCTC CTCCATCCAA AAAGACGAAG 
GATGAAGCCC CCGAAGACGA GGAAGAAGAT GAAGAAGAGG ACGGCTCAGA AGAGGAGGCT 
CCATCCGGGG AAGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Identification and characterization of two putative nuclear localization signals (NLS) in the DNA-binding protein NUCKS.
Biochimica et biophysica acta1773(9)1398-406(2007 Sep)
Grundt K,Haga IV,Huitfeldt HS,Ostvold AC

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

Characterisation of the NUCKS gene on human chromosome 1q32.1 and the presence of a homologous gene in different species.
Biochemical and biophysical research communications323(3)796-801(2004 Oct)
Grundt K,Haga IV,Aleporou-Marinou V,Drosos Y,Wanvik B,Østvold AC

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nucks1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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