pfkma cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pfkma
Entrez Gene ID	447836
Full Name	phosphofructokinase, muscle a
Synonyms	pfkm,zgc:92344
General protein information	Preferred Names phosphofructokinase, muscle a Names ATP-dependent 6-phosphofructokinase, muscle type
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001004575.1	NP_001004575.1	ATP-dependent 6-phosphofructokinase, muscle type
NM_001004575.2	NP_001004575.1	ATP-dependent 6-phosphofructokinase, muscle type

Pathways from BioSystems

Homologies

Homo sapiens (human)	PFKM	NP_001160158.1
Rattus norvegicus (Norway rat)	Pfkm	NP_113903.1
Mus musculus (house mouse)	Pfkm	NP_067489.3
Xenopus tropicalis (tropical clawed frog)	pfkm	XP_004911968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007642	XP_564589.3
Pan troglodytes (chimpanzee)	PFKM	XP_509029.2
Macaca mulatta (Rhesus monkey)	PFKM	XP_001096244.1
Canis lupus familiaris (dog)	PFKM	NP_001003199.1
Bos taurus (cattle)	PFKM	NP_001068736.1
Drosophila melanogaster (fruit fly)	Pfk	NP_724891.2
Caenorhabditis elegans (roundworm)	Y71H10A.1	NP_741738.1
Gallus gallus (chicken)	PFKM	NP_989554.2
Danio rerio (zebrafish)	pfkmb	NP_001082886.1
Danio rerio (zebrafish)	pfkma	NP_001004575.1

Gene Ontology
Bibliography

Related articles in PubMed

Different roles of insulin receptor a and b in maintaining blood glucose homeostasis in zebrafish.
Gong Y, Zhai G, Su J, Yang B, Jin J, Liu H, Yin Z, Xie S, Han D
General and comparative endocrinology26933-45(2018 12)

Inhibited Carnitine Synthesis Causes Systemic Alteration of Nutrient Metabolism in Zebrafish.
Li JM, Li LY, Qin X, Degrace P, Demizieux L, Limbu SM, Wang X, Zhang ML, Li DL, Du ZY
Frontiers in physiology9509(2018)

Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise.
Boskovic S, Marín-Juez R, Jasnic J, Reischauer S, El Sammak H, Kojic A, Faulkner G, Radojkovic D, Stainier DYR, Kojic S
PloS one13(9)e0204312(2018)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Systemic regulation of L-carnitine in nutritional metabolism in zebrafish, Danio rerio.
Li JM, Li LY, Qin X, Ning LJ, Lu DL, Li DL, Zhang ML, Wang X, Du ZY
Scientific reports740815(2017 01)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pfkma gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pfkma cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa159775	NM_001004575.2 Latest version!	Danio rerio phosphofructokinase, muscle a (pfkma), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa36036	NM_001004575.1 Latest version!	Danio rerio phosphofructokinase, muscle a (pfkma), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pfkma ( NM_001004575.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001004575.1 Danio rerio phosphofructokinase, muscle a (pfkma), mRNA.

Price & Availability↑

CloneID	ODa159775
Clone ID Related Accession (Same CDS sequence)	NM_001004575.2
Accession Version	NM_001004575.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555862400000
Organism	Danio rerio(zebrafish)
Product	ATP-dependent 6-phosphofructokinase, muscle type
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR354381.18. On Aug 17, 2018 this sequence version replaced NM_001004575.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081652.1, GFIL01030026.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTAACA TAAACACGAC TGAGGTGGAT CCAACAAAGA TGGGGGTCGG ACGGGCCATC 
GCTGTGCTGA CGTCAGGTGG TGATGCCCAG GGGATGAATG CAGCTGTGAG GGCCACTGTC 
AGAGTTGGTC TTTACACTGG AGCTAAAGTC TTCTTCGTCC ATGAGGGCTA TCAGGGATTG 
GTTGATGGAG GGGATCACAT CCGTCCTGCT ACATGGGAGA GTGTGTCCAT GATGCTGCAG 
CTGGGTGGGA CAGTAATTGG CAGCGCCCGC TGTAAGGATT TCCAGACCAG AGAAGGGCGT 
TTAAAGGCTG CATACAATCT TGTCAAATTA GGCATCACTA ACCTCTGTGT AATCGGCGGT 
GATGGCAGTC TGACCGGTGC CAACATATTT CGCACAGAGT GGAGTGACTT GCTGAAAGAT 
CATGTCAGCA AAGGTAAGAT CACCAAGGAA GAGGCCAAAG CTTCATCTCA TCTGAATATT 
GTGGGTATGG TTGGCTCCAT AGACAATGAC TTTTGTGGAA CGGACATGAC TATTGGCACA 
GACTCGGCCC TGCATCGCAT CATTGAGGTG GTGGACAGTA TCACCACAAC AGCACAAAGC 
CACCAACGTG CGTTTATCCT GGAGGTCATG GGCAGACACT GTGGCTATCT AGCACTGGTT 
ACTGCTCTTG CATGTGGTGC CGACTGGGTG TTTATTCCAG AGATGCCTCC AGATGATGGC 
TGGGAGGAGC ACTTGTGCAG AAGACTAACA GAGACCAGAA ATGCAGGTTC TCGTCTCAAT 
GTGATCATCG TGGCTGAGGG AGCCATCAAT AAAGAAGGAC AACCAATCAC CTGTGACCAG 
CTGAAAGATC TGGTGACAAA GAGGCTGGGC TTTGACACTC GTGCCACTAT TCTTGGACAC 
GTGCAGAGAG GTGGAACCCC TTCAGCTTTT GACAGAGTGC TAGGCAGTCG GATGGGTGTG 
GAGGCTGTAA TGGCTCTGCT GGAAGCCACT CCAGAGACCC CTGCGTGTGT GGTCAGTCTG 
ACCGGGAACC AGGCTGTTAG ACTCCCCCTG ATGGAGTGTG TACAAGTGAC CAAGGATGTC 
ACTATTGCAA TGAATGAGGG TCGATTCGAT GATGCTGTAA AGCTCAGAGG AAGGAGTTTT 
GAGAATAACT GGAACACATA TAAGCTTTTG GCTCATATTC GCCCCCCAGA TAAGAAGAGT 
AACATTAATG TGGCCATCCT GAACGTGGGT GCTCCCTGCG CTGGCATGAA CGCAGCTGTG 
CGTGCAGCTG TTAGGATCGG CATCATCCAA GGCCACAACA TGCTGGCGGT CCATGATGGC 
TTTGAGGGTC TGGCTCAAGC AAAGATTGAG CCTATCACCT GGAACACTGT TGGAGATTGG 
ACAGGGAAAG GTGGATCTGA CTTGGGAACT AAAAGACTAT TGCCTGGAAA ATACATGGAG 
GAGATCAGCC TGAATATTGC CAAGTATAAT ATTCACGCTC TGGTTATCAT CGGTGGTTTT 
GAGGCATTTT CAGGTGGACT GGAAATGGTT CAAGGCAGAG AAAAGTATGA AGAGCTGTGT 
ATTCCTATGG TGATCATTCC TGCTACTGTA TCAAACAACG TGCCCGGCTC AGACTTCAGC 
ATTGGAGCAG ACACTGCCCT TAATACCATC ACCACGACCT GTGACAGGAT CAAGCAGTCG 
GCAGCAGGAA CTAAGCGACG TGTGTTCATC ATTGAAACTA TGGGAGGATA CTGCGGTTAC 
CTGGCCACAA TGTCTGGTCT CTCTGCCGGA GCTGATGCTG CTTACATATT CGAGGAGAAA 
TTTGGCATTC GTGACCTAGA GAGAAATGTG GAACATCTTG TTGAGAAGAT GAAAACCACT 
GTTAAGAGAG GCCTAATTCT CAGAAATGAG AACAGCAATT CCAACTACAC CACCGATTTC 
ATCTTCAACC TGTATTCAGA GGAAGGGAAA GGTGTTTTTG ACTGCCGTAA GAATGTCCTT 
GGCCATATGC AGCAGGGTGG CACCCCAACA CCTTTTGATA GAAACTTTGC TACCAAAATG 
GGTGCCAAGG CTGTTCTCTG GCTGACAGAA AAACTGAAGG ATTGTTATAG ACATGGGCGA 
ATCTTTGCCA ACACCCCAGA CTCTGCATGT GTGTTAGGCA TGAGGAAGAG AGGACTGGTG 
TTACAGCCTC TAGCTGAATT GAAAGAAGAG ACCGATTTTG AACACCGTAT ACCAAAGAGC 
CAGTGGTGGT TAAAGCTGAG GCCTATTATG AAGATCCTGG CCAAGTACAA GATTAATCTG 
GACACCTCTG AACATGCTCT AATGGAGCAC GTTGTCAGCA AGAAGCCTCC GGTGCACATG 
ACCCTCACAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004575.1
CDS	89..2443
Translation	MANINTTEVDPTKMGVGRAIAVLTSGGDAQGMNAAVRATVRVGLYTGAKVFFVHEGYQGLVDGGDHIRPATWESVSMMLQLGGTVIGSARCKDFQTREGRLKAAYNLVKLGITNLCVIGGDGSLTGANIFRTEWSDLLKDHVSKGKITKEEAKASSHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDSITTTAQSHQRAFILEVMGRHCGYLALVTALACGADWVFIPEMPPDDGWEEHLCRRLTETRNAGSRLNVIIVAEGAINKEGQPITCDQLKDLVTKRLGFDTRATILGHVQRGGTPSAFDRVLGSRMGVEAVMALLEATPETPACVVSLTGNQAVRLPLMECVQVTKDVTIAMNEGRFDDAVKLRGRSFENNWNTYKLLAHIRPPDKKSNINVAILNVGAPCAGMNAAVRAAVRIGIIQGHNMLAVHDGFEGLAQAKIEPITWNTVGDWTGKGGSDLGTKRLLPGKYMEEISLNIAKYNIHALVIIGGFEAFSGGLEMVQGREKYEELCIPMVIIPATVSNNVPGSDFSIGADTALNTITTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMSGLSAGADAAYIFEEKFGIRDLERNVEHLVEKMKTTVKRGLILRNENSNSNYTTDFIFNLYSEEGKGVFDCRKNVLGHMQQGGTPTPFDRNFATKMGAKAVLWLTEKLKDCYRHGRIFANTPDSACVLGMRKRGLVLQPLAELKEETDFEHRIPKSQWWLKLRPIMKILAKYKINLDTSEHALMEHVVSKKPPVHMTLTK

Target ORF information:

RefSeq Version	NM_001004575.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphofructokinase, muscle a (pfkma), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004575.2

ORF Insert Sequence:

ATGGCTAACA TAAACACGAC TGAGGTGGAT CCAACAAAGA TGGGGGTCGG ACGGGCCATC 
GCTGTGCTGA CGTCAGGTGG TGATGCCCAG GGGATGAATG CAGCTGTGAG GGCCACTGTC 
AGAGTTGGTC TTTACACTGG AGCTAAAGTC TTCTTCGTCC ATGAGGGCTA TCAGGGATTG 
GTTGATGGAG GGGATCACAT CCGTCCTGCT ACATGGGAGA GTGTGTCCAT GATGCTGCAG 
CTGGGTGGGA CAGTAATTGG CAGCGCCCGC TGTAAGGATT TCCAGACCAG AGAAGGGCGT 
TTAAAGGCTG CATACAATCT TGTCAAATTA GGCATCACTA ACCTCTGTGT AATCGGCGGT 
GATGGCAGTC TGACCGGTGC CAACATATTT CGCACAGAGT GGAGTGACTT GCTGAAAGAT 
CATGTCAGCA AAGGTAAGAT CACCAAGGAA GAGGCCAAAG CTTCATCTCA TCTGAATATT 
GTGGGTATGG TTGGCTCCAT AGACAATGAC TTTTGTGGAA CGGACATGAC TATTGGCACA 
GACTCGGCCC TGCATCGCAT CATTGAGGTG GTGGACAGTA TCACCACAAC AGCACAAAGC 
CACCAACGTG CGTTTATCCT GGAGGTCATG GGCAGACACT GTGGCTATCT AGCACTGGTT 
ACTGCTCTTG CATGTGGTGC CGACTGGGTG TTTATTCCAG AGATGCCTCC AGATGATGGC 
TGGGAGGAGC ACTTGTGCAG AAGACTAACA GAGACCAGAA ATGCAGGTTC TCGTCTCAAT 
GTGATCATCG TGGCTGAGGG AGCCATCAAT AAAGAAGGAC AACCAATCAC CTGTGACCAG 
CTGAAAGATC TGGTGACAAA GAGGCTGGGC TTTGACACTC GTGCCACTAT TCTTGGACAC 
GTGCAGAGAG GTGGAACCCC TTCAGCTTTT GACAGAGTGC TAGGCAGTCG GATGGGTGTG 
GAGGCTGTAA TGGCTCTGCT GGAAGCCACT CCAGAGACCC CTGCGTGTGT GGTCAGTCTG 
ACCGGGAACC AGGCTGTTAG ACTCCCCCTG ATGGAGTGTG TACAAGTGAC CAAGGATGTC 
ACTATTGCAA TGAATGAGGG TCGATTCGAT GATGCTGTAA AGCTCAGAGG AAGGAGTTTT 
GAGAATAACT GGAACACATA TAAGCTTTTG GCTCATATTC GCCCCCCAGA TAAGAAGAGT 
AACATTAATG TGGCCATCCT GAACGTGGGT GCTCCCTGCG CTGGCATGAA CGCAGCTGTG 
CGTGCAGCTG TTAGGATCGG CATCATCCAA GGCCACAACA TGCTGGCGGT CCATGATGGC 
TTTGAGGGTC TGGCTCAAGC AAAGATTGAG CCTATCACCT GGAACACTGT TGGAGATTGG 
ACAGGGAAAG GTGGATCTGA CTTGGGAACT AAAAGACTAT TGCCTGGAAA ATACATGGAG 
GAGATCAGCC TGAATATTGC CAAGTATAAT ATTCACGCTC TGGTTATCAT CGGTGGTTTT 
GAGGCATTTT CAGGTGGACT GGAAATGGTT CAAGGCAGAG AAAAGTATGA AGAGCTGTGT 
ATTCCTATGG TGATCATTCC TGCTACTGTA TCAAACAACG TGCCCGGCTC AGACTTCAGC 
ATTGGAGCAG ACACTGCCCT TAATACCATC ACCACGACCT GTGACAGGAT CAAGCAGTCG 
GCAGCAGGAA CTAAGCGACG TGTGTTCATC ATTGAAACTA TGGGAGGATA CTGCGGTTAC 
CTGGCCACAA TGTCTGGTCT CTCTGCCGGA GCTGATGCTG CTTACATATT CGAGGAGAAA 
TTTGGCATTC GTGACCTAGA GAGAAATGTG GAACATCTTG TTGAGAAGAT GAAAACCACT 
GTTAAGAGAG GCCTAATTCT CAGAAATGAG AACAGCAATT CCAACTACAC CACCGATTTC 
ATCTTCAACC TGTATTCAGA GGAAGGGAAA GGTGTTTTTG ACTGCCGTAA GAATGTCCTT 
GGCCATATGC AGCAGGGTGG CACCCCAACA CCTTTTGATA GAAACTTTGC TACCAAAATG 
GGTGCCAAGG CTGTTCTCTG GCTGACAGAA AAACTGAAGG ATTGTTATAG ACATGGGCGA 
ATCTTTGCCA ACACCCCAGA CTCTGCATGT GTGTTAGGCA TGAGGAAGAG AGGACTGGTG 
TTACAGCCTC TAGCTGAATT GAAAGAAGAG ACCGATTTTG AACACCGTAT ACCAAAGAGC 
CAGTGGTGGT TAAAGCTGAG GCCTATTATG AAGATCCTGG CCAAGTACAA GATTAATCTG 
GACACCTCTG AACATGCTCT AATGGAGCAC GTTGTCAGCA AGAAGCCTCC GGTGCACATG 
ACCCTCACAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkma ( NM_001004575.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001004575.1 Danio rerio phosphofructokinase, muscle a (pfkma), mRNA.

Price & Availability↑

CloneID	ODa36036
Clone ID Related Accession (Same CDS sequence)	NM_001004575.1
Accession Version	NM_001004575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1501862400000
Organism	Danio rerio(zebrafish)
Product	ATP-dependent 6-phosphofructokinase, muscle type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081652.1. ##Evidence-Data-START## Transcript exon combination :: BC081652.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTAACA TAAACACGAC TGAGGTGGAT CCAACAAAGA TGGGGGTCGG ACGGGCCATC 
GCTGTGCTGA CGTCAGGTGG TGATGCCCAG GGGATGAATG CAGCTGTGAG GGCCACTGTC 
AGAGTTGGTC TTTACACTGG AGCTAAAGTC TTCTTCGTCC ATGAGGGCTA TCAGGGATTG 
GTTGATGGAG GGGATCACAT CCGTCCTGCT ACATGGGAGA GTGTGTCCAT GATGCTGCAG 
CTGGGTGGGA CAGTAATTGG CAGCGCCCGC TGTAAGGATT TCCAGACCAG AGAAGGGCGT 
TTAAAGGCTG CATACAATCT TGTCAAATTA GGCATCACTA ACCTCTGTGT AATCGGCGGT 
GATGGCAGTC TGACCGGTGC CAACATATTT CGCACTGAGT GGAGTGACTT GCTGAAAGAT 
CATGTCAGCA AAGGTAAGAT CACCAAGGAA GAGGCCAAAG CTTCATCTCA TCTGAATATT 
GTGGGTATGG TTGGCTCCAT AGACAATGAC TTTTGTGGAA CGGACATGAC TATTGGCACA 
GACTCGGCCC TGCATCGCAT CATTGAGGTG GTGGACAGTA TCACCACAAC AGCACAAAGC 
CACCAACGTG CGTTTATCCT GGAGGTCATG GGCAGACACT GTGGCTATCT AGCACTGGTT 
ACTGCTCTTG CATGTGGTGC CGACTGGGTG TTTATTCCAG AGATGCCTCC AGATGATGGC 
TGGGAGGAGC ACTTGTGCAG AAGACTAACA GAGACCAGAA ATGCAGGTTC TCGTCTCAAT 
GTGATCATCG TGGCTGAGGG AGCCATCAAT AAAGAAGGAC AACCAATCAC CTGTGACCAG 
CTGAAAGATC TGGTGACAAA GAGGCTGGGC TTTGACACTC GTGCCACTAT TCTTGGACAC 
GTGCAGAGAG GTGGAACCCC TTCAGCTTTT GACAGAGTCC TAGGCAGTCG GATGGGTGTG 
GAGGCTGTAA TGGCTCTGCT GGAAGCCACT CCAGAGACCC CTGCGTGTGT GGTCAGTCTG 
ACCGGGAACC AGGCTGTTAG ACTCCCCCTG ATGGAGTGTG TACAAGTGAC CAAGGATGTC 
ACTATTGCAA TGAATGAGGG TCGATTCGAT GATGCTGTAA AGCTCAGGGG AAGGAGTTTT 
GAGAATAACT GGAACACATA TAAGCTTTTG GCTCATATTC GCCCCCCAGA TAAGAAGAGT 
AACATTAATG TGGCCATCCT GAACGTGGGT GCTCCCTGCG CTGGCATGAA CGCAGCTGTG 
CGTGCAGCTG TCAGGATCGG CATCATCCAA GGCCACAACA TGCTGGCGGT CCATGATGGC 
TTTGAGGGTC TGGCTCAAGC AAAGATTGAG CCTATCACCT GGAACACTGT TGGAGATTGG 
ACAGGGAAAG GAGGATCTGA CTTGGGAACT AAAAGACTAT TGCCTGGAAA ATACATGGAG 
GAGATCAGCC TGAATATTGC CAAGTATAAT ATTCACGCTC TGGTTATCAT CGGTGGTTTT 
GAGGCATTTT CAGGTGGACT GGAAATGGTT CAAGGCAGAG AAAAGTATGA AGAGCTGTGT 
ATTCCTATGG TGATCATTCC TGCTACTGTA TCAAACAACG TGCCCGGCTC AGACTTCAGC 
ATTGGAGCAG ACACTGCCCT TAATACCATC ACCACGACCT GTGACAGGAT CAAGCAGTCG 
GCAGCAGGAA CTAAGCGACG TGTGTTCATC ATTGAAACTA TGGGAGGATA CTGCGGTTAC 
CTGGCCACAA TGTCTGGTCT CTCTGCCGGA GCTGATGCTG CTTACATATT CGAGGAGAAA 
TTTGGCATTC GTGACCTAGA GAGAAATGTG GAACATCTTG TTGAGAAGAT GAAAACCACT 
GTTAAGAGAG GCCTAATTCT CAGAAATGAG AACAGCAATT CCAACTACAC CACCGATTTC 
ATCTTCAACC TGTATTCAGA GGAAGGGAAA GGTGTTTTTG ACTGCCGTAA GAATGTCCTT 
GGCCATATGC AGCAGGGTGG CACCCCAACA CCTTTTGATA GAAACTTTGC TACCAAAATG 
GGTGCCAAGG CTGTTCTCTG GCTGACAGAA AAACTGAAGG ATTGTTATAG ACATGGGCGA 
ATCTTTGCCA ACACCCCAGA CTCTGCATGT GTGTTAGGCA TGAGGAAGAG AGGACTGGTG 
TTACAGCCTC TAGCTGAATT GAAAGAAGAG ACCGATTTTG AACACCGTAT ACCAAAGAGC 
CAGTGGTGGT TAAAGCTGAG GCCTATTATG AAGATCCTGG CCAAGTACAA GATTAATCTG 
GACACCTCTG AACATGCTCT AATGGAGCAC GTTGTCAGCA AGAAGCCTCC GGTGCACATG 
ACCCTCACAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004575.1
CDS	65..2419
Translation	MANINTTEVDPTKMGVGRAIAVLTSGGDAQGMNAAVRATVRVGLYTGAKVFFVHEGYQGLVDGGDHIRPATWESVSMMLQLGGTVIGSARCKDFQTREGRLKAAYNLVKLGITNLCVIGGDGSLTGANIFRTEWSDLLKDHVSKGKITKEEAKASSHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDSITTTAQSHQRAFILEVMGRHCGYLALVTALACGADWVFIPEMPPDDGWEEHLCRRLTETRNAGSRLNVIIVAEGAINKEGQPITCDQLKDLVTKRLGFDTRATILGHVQRGGTPSAFDRVLGSRMGVEAVMALLEATPETPACVVSLTGNQAVRLPLMECVQVTKDVTIAMNEGRFDDAVKLRGRSFENNWNTYKLLAHIRPPDKKSNINVAILNVGAPCAGMNAAVRAAVRIGIIQGHNMLAVHDGFEGLAQAKIEPITWNTVGDWTGKGGSDLGTKRLLPGKYMEEISLNIAKYNIHALVIIGGFEAFSGGLEMVQGREKYEELCIPMVIIPATVSNNVPGSDFSIGADTALNTITTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMSGLSAGADAAYIFEEKFGIRDLERNVEHLVEKMKTTVKRGLILRNENSNSNYTTDFIFNLYSEEGKGVFDCRKNVLGHMQQGGTPTPFDRNFATKMGAKAVLWLTEKLKDCYRHGRIFANTPDSACVLGMRKRGLVLQPLAELKEETDFEHRIPKSQWWLKLRPIMKILAKYKINLDTSEHALMEHVVSKKPPVHMTLTK

Target ORF information:

RefSeq Version	NM_001004575.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphofructokinase, muscle a (pfkma), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004575.1

ORF Insert Sequence:

ATGGCTAACA TAAACACGAC TGAGGTGGAT CCAACAAAGA TGGGGGTCGG ACGGGCCATC 
GCTGTGCTGA CGTCAGGTGG TGATGCCCAG GGGATGAATG CAGCTGTGAG GGCCACTGTC 
AGAGTTGGTC TTTACACTGG AGCTAAAGTC TTCTTCGTCC ATGAGGGCTA TCAGGGATTG 
GTTGATGGAG GGGATCACAT CCGTCCTGCT ACATGGGAGA GTGTGTCCAT GATGCTGCAG 
CTGGGTGGGA CAGTAATTGG CAGCGCCCGC TGTAAGGATT TCCAGACCAG AGAAGGGCGT 
TTAAAGGCTG CATACAATCT TGTCAAATTA GGCATCACTA ACCTCTGTGT AATCGGCGGT 
GATGGCAGTC TGACCGGTGC CAACATATTT CGCACTGAGT GGAGTGACTT GCTGAAAGAT 
CATGTCAGCA AAGGTAAGAT CACCAAGGAA GAGGCCAAAG CTTCATCTCA TCTGAATATT 
GTGGGTATGG TTGGCTCCAT AGACAATGAC TTTTGTGGAA CGGACATGAC TATTGGCACA 
GACTCGGCCC TGCATCGCAT CATTGAGGTG GTGGACAGTA TCACCACAAC AGCACAAAGC 
CACCAACGTG CGTTTATCCT GGAGGTCATG GGCAGACACT GTGGCTATCT AGCACTGGTT 
ACTGCTCTTG CATGTGGTGC CGACTGGGTG TTTATTCCAG AGATGCCTCC AGATGATGGC 
TGGGAGGAGC ACTTGTGCAG AAGACTAACA GAGACCAGAA ATGCAGGTTC TCGTCTCAAT 
GTGATCATCG TGGCTGAGGG AGCCATCAAT AAAGAAGGAC AACCAATCAC CTGTGACCAG 
CTGAAAGATC TGGTGACAAA GAGGCTGGGC TTTGACACTC GTGCCACTAT TCTTGGACAC 
GTGCAGAGAG GTGGAACCCC TTCAGCTTTT GACAGAGTCC TAGGCAGTCG GATGGGTGTG 
GAGGCTGTAA TGGCTCTGCT GGAAGCCACT CCAGAGACCC CTGCGTGTGT GGTCAGTCTG 
ACCGGGAACC AGGCTGTTAG ACTCCCCCTG ATGGAGTGTG TACAAGTGAC CAAGGATGTC 
ACTATTGCAA TGAATGAGGG TCGATTCGAT GATGCTGTAA AGCTCAGGGG AAGGAGTTTT 
GAGAATAACT GGAACACATA TAAGCTTTTG GCTCATATTC GCCCCCCAGA TAAGAAGAGT 
AACATTAATG TGGCCATCCT GAACGTGGGT GCTCCCTGCG CTGGCATGAA CGCAGCTGTG 
CGTGCAGCTG TCAGGATCGG CATCATCCAA GGCCACAACA TGCTGGCGGT CCATGATGGC 
TTTGAGGGTC TGGCTCAAGC AAAGATTGAG CCTATCACCT GGAACACTGT TGGAGATTGG 
ACAGGGAAAG GAGGATCTGA CTTGGGAACT AAAAGACTAT TGCCTGGAAA ATACATGGAG 
GAGATCAGCC TGAATATTGC CAAGTATAAT ATTCACGCTC TGGTTATCAT CGGTGGTTTT 
GAGGCATTTT CAGGTGGACT GGAAATGGTT CAAGGCAGAG AAAAGTATGA AGAGCTGTGT 
ATTCCTATGG TGATCATTCC TGCTACTGTA TCAAACAACG TGCCCGGCTC AGACTTCAGC 
ATTGGAGCAG ACACTGCCCT TAATACCATC ACCACGACCT GTGACAGGAT CAAGCAGTCG 
GCAGCAGGAA CTAAGCGACG TGTGTTCATC ATTGAAACTA TGGGAGGATA CTGCGGTTAC 
CTGGCCACAA TGTCTGGTCT CTCTGCCGGA GCTGATGCTG CTTACATATT CGAGGAGAAA 
TTTGGCATTC GTGACCTAGA GAGAAATGTG GAACATCTTG TTGAGAAGAT GAAAACCACT 
GTTAAGAGAG GCCTAATTCT CAGAAATGAG AACAGCAATT CCAACTACAC CACCGATTTC 
ATCTTCAACC TGTATTCAGA GGAAGGGAAA GGTGTTTTTG ACTGCCGTAA GAATGTCCTT 
GGCCATATGC AGCAGGGTGG CACCCCAACA CCTTTTGATA GAAACTTTGC TACCAAAATG 
GGTGCCAAGG CTGTTCTCTG GCTGACAGAA AAACTGAAGG ATTGTTATAG ACATGGGCGA 
ATCTTTGCCA ACACCCCAGA CTCTGCATGT GTGTTAGGCA TGAGGAAGAG AGGACTGGTG 
TTACAGCCTC TAGCTGAATT GAAAGAAGAG ACCGATTTTG AACACCGTAT ACCAAAGAGC 
CAGTGGTGGT TAAAGCTGAG GCCTATTATG AAGATCCTGG CCAAGTACAA GATTAATCTG 
GACACCTCTG AACATGCTCT AATGGAGCAC GTTGTCAGCA AGAAGCCTCC GGTGCACATG 
ACCCTCACAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Different roles of insulin receptor a and b in maintaining blood glucose homeostasis in zebrafish.
General and comparative endocrinology26933-45(2018 12)
Gong Y,Zhai G,Su J,Yang B,Jin J,Liu H,Yin Z,Xie S,Han D

Inhibited Carnitine Synthesis Causes Systemic Alteration of Nutrient Metabolism in Zebrafish.
Frontiers in physiology9509(2018)
Li JM,Li LY,Qin X,Degrace P,Demizieux L,Limbu SM,Wang X,Zhang ML,Li DL,Du ZY

Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise.
PloS one13(9)e0204312(2018)
Boskovic S,Marín-Juez R,Jasnic J,Reischauer S,El Sammak H,Kojic A,Faulkner G,Radojkovic D,Stainier DYR,Kojic S

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Systemic regulation of L-carnitine in nutritional metabolism in zebrafish, Danio rerio.
Scientific reports740815(2017 01)
Li JM,Li LY,Qin X,Ning LJ,Lu DL,Li DL,Zhang ML,Wang X,Du ZY

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pfkma cDNA ORF clone, Danio rerio(zebrafish)

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