mastl cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mastl
Entrez Gene ID	445215
Full Name	microtubule associated serine/threonine kinase-like
Synonyms	GW,GWL,MAST-L,im:7149025,wu:fa02a12,zgc:100990
General protein information	Preferred Names microtubule associated serine/threonine kinase-like Names serine/threonine-protein kinase greatwall microtubule-associated serine/threonine-protein kinase-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021470139.1	XP_021325814.1	serine/threonine-protein kinase greatwall isoform X1
NM_001003609.2	NP_001003609.2	serine/threonine-protein kinase greatwall

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mastl	NP_080255.3
Rattus norvegicus (Norway rat)	Mastl	NP_001100839.1
Gallus gallus (chicken)	MASTL	XP_418589.2
Homo sapiens (human)	MASTL	NP_001165774.1
Pan troglodytes (chimpanzee)	MASTL	XP_001160397.3
Macaca mulatta (Rhesus monkey)	MASTL	XP_001104617.1
Canis lupus familiaris (dog)	MASTL	XP_848866.1
Bos taurus (cattle)	MASTL	NP_001107237.1
Danio rerio (zebrafish)	mastl	NP_001003609.2
Xenopus tropicalis (tropical clawed frog)	mastl	NP_001120546.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Briolat V, Jouneau L, Carvalho R, Palha N, Langevin C, Herbomel P, Schwartz O, Spaink HP, Levraud JP, Boudinot P
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)

In vivo inactivation of MASTL kinase results in thrombocytopenia.
Johnson HJ, Gandhi MJ, Shafizadeh E, Langer NB, Pierce EL, Paw BH, Gilligan DM, Drachman JG
Experimental hematology37(8)901-8(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mastl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mastl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa39004	NM_001003609.2 Latest version!	Danio rerio microtubule associated serine/threonine kinase-like (mastl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa151671	XM_021470139.1 Latest version!	Danio rerio microtubule associated serine/threonine kinase-like (mastl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mastl ( NM_001003609.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003609.2 Danio rerio microtubule associated serine/threonine kinase-like (mastl), mRNA.

Price & Availability↑

CloneID	ODa39004
Clone ID Related Accession (Same CDS sequence)	NM_001003609.2
Accession Version	NM_001003609.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase greatwall
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT573382.3. On Nov 25, 2009 this sequence version replaced NM_001003609.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01025975.1, BC078324.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476729, SAMEA4476732 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGCTC GTGGATTGGC ATGCGAAAGC TCAAACAGTG CAGTGAAAGC TCCTTCCATC 
GAGGACTTCG TGCTCGTCAA ACCCATCAGT CGAGGAGCTT TCGGGAAGGT TTACCTCGCC 
AGAAAGAAAT GCAACTCCAA ACTCTATGCC ATCAAGGTGG TGAAGAAAGC AGAAATGGTG 
GATAAGAACA TGACTGAGCA GATGAGAGCT GAGAGAGATG CTCTGGCTCT CAGTAAAAGC 
CCTTTCATTG TGCATCTGTT TTACTCCCTT CAAACCGCAA CTAAAGTCTA TCTGGTGATG 
GAGTATCTGA TTGGAGGAGA TGTGAAATCG CTTCTTCATA TTTATGGCTA CTTTGATGAG 
GACATGTCTC TGAAATACAT CTCAGAGGTC GCGCTCGCAC TGGATTACCT TCACAGACAC 
AGCATTATTC ACAGGGATTT AAAACCAGAC AACATGCTCA TCTCGAACGA GGGTCACATT 
AAGCTTACAG ACTTTGGCCT CTCCAAGGTT AAACTCGACA GAGAGCTAAA CCTGATGGAC 
ATTTTAACCA CACCATCTTT GGTGAAGCCT ACAAAGGATT ATTTTCGAAC ACCAGGTCAA 
GTTCTGTCAC TGATCAGCTC TCTTGGTCTT AACACCCCAG TTATAGAGGG TAAGCGTCAC 
AGCAGCACAG TACTGGGCAG CCCAATGTCC TGTGGGAAAG TCAAGCAGAG GAACAGGTCT 
CTGGGCTCAC CTCTCATGAA GAGACGGGCC GAGTACATGA ATTCCCCAGT GTGCACAAGT 
CGAGCTCTAG CATCCAACAG TGTGTTCAGT CCTGTCCTGC TGGCGAGGAG TTTGACACCA 
AGGCTGTTAA AGTCAGGGAA GAGACTCGAT ACTATGAGTG TTGGGAGCAC ACACTCATGC 
ATGCTACCAT CCACTACTGA CTCTGAAAAC TGCGTCAGCC CTATGTGGGA AGATGAGCAG 
AACTTGCATG ATGTCGAGAA CATTCCACAG CTGAATGGAA GAGAAGTTGA CAACAGAAAG 
TCAAGAAACG TTCCCTTGAC TCCTGTGGAA AAGAGGAAGA CGCTTCCAGC GCGAGTTCAG 
GATCACAGCA CTGTTTTTAC TCCCCTCAAT AACCAAGTTA ACAACAGCAG GAATATTAAA 
CCAGACCTTT CTGCAAAAAG GCTCCAGTTT GGTGCAACTG ACAACTCTGC CACTAAATTG 
GAGAAGACTG TTCCTCATCA GTCAGTTGGG AATGGTCTTA TAAAACCAGA GCCTCTAAAA 
GAGCTCAAAT CTTCAGTGAA GAGAACATTT GAGGAGGTTG AGAAAAGCCC AGAACAGGCT 
GAGATTTTAT GCAAGAAAAA TGACGCAGCG TACAAGAGAT CCTTCCAGAT TCCTGAGAAG 
ACCTCAAGGG CCCACACAGG CTTGACTGGA ATTTTCTCGA TTGTAGGGCT TGACGATGTT 
AAAAGTGCAC CTAAATGTCA ACAGTTCAAT GAGAGAACTG GCCCAAAGCA GTCTAGTCCC 
ATAGCTGTGG CCAAAAACCT CTTCTGTGAA CTGGAAGATC AGGGGGAAGA GGGGGGTAAA 
GCAGAGCCCA ACTCCAGCTC TTCAACCTCG CCCGGTGATG AGCGAAACAT CAGAAGAAGC 
CTAAGTTTAG AGTCTGACGT CTCAGCACAT GAGATGTCAC TGGTTGCTAA CACGCCACAA 
AAACTGTCAG ACGCCAAACA AGAAGTTTTA TCTTCGTCTT TTGAGGAGTT GGACGAAAAC 
GAGATTTCTG CAGTCACCCC GATGGCCAGA CCCACGGTTG CTACACCAAA GCATAGCAGC 
GCAAAGCAGC GCAGGGGTGA ATGTGAGCGT TCTCTGCTTG ATCATCCTCA TGGCCTGTCT 
GACAGTATGA TCAAATCACC CGGCTTCCTC AAACCCAAAA ATGTGGTTGC GTTTAGGAGC 
TACTGTAGCT CAATCAACCG CTCATGTACG TCACATCTCA GCCTGGCCTC GTTTGATGCC 
ATGGAAATGT CTGCGTCAGC GTCCTTTCAC AACGCTGTGA CTCCAGTTCA GAAGAAAAGG 
CCCAGTCTAA ACAACTCTCT TTACCAGACT CCCCAGCAGA TGGTTGTGTC TCACACTCCC 
TATAGGACAC CTAAAAGTGT CCGTAGAGGT CCTGAACGTG TTGAAGGGGC TCCTATTTTG 
GGTACTCCTG ACTACTTGGC ACCAGAACTT TTATTAGGCA AGCCTCATGA TTTCATGGTG 
GACTGGTGGG CATTGGGTGT TTGTCTGTTC GAGTTTCTCA CGGGAGTCCC ACCTTTCAAC 
GATGAGACCC CTCAATTGGT GTTTCAGAAC ATTCTCAACA GAGATATACC ATGGCCAGAC 
GGAGAAGAGG AGTTGACTCC AAATTCAAGA AATGCCATTG AGATTCTGCT CACCATGGAC 
ACACTCAAAC GGGCCGGCTT AAAAGAGTTG AAGGATCACC CGTTTTTTGA TGGTGTGGAC 
TGGGAAAACC TACACCATCA GACGATGCCC TTCATCCCAC AGCCTGACAA CGAGACTGAC 
ACATCGTACT TTGAGGCCAG GAATACGGCA CAACATCTGA CCGTTTCCGG CTTCAGTCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003609.2
CDS	284..2866
Translation	MEARGLACESSNSAVKAPSIEDFVLVKPISRGAFGKVYLARKKCNSKLYAIKVVKKAEMVDKNMTEQMRAERDALALSKSPFIVHLFYSLQTATKVYLVMEYLIGGDVKSLLHIYGYFDEDMSLKYISEVALALDYLHRHSIIHRDLKPDNMLISNEGHIKLTDFGLSKVKLDRELNLMDILTTPSLVKPTKDYFRTPGQVLSLISSLGLNTPVIEGKRHSSTVLGSPMSCGKVKQRNRSLGSPLMKRRAEYMNSPVCTSRALASNSVFSPVLLARSLTPRLLKSGKRLDTMSVGSTHSCMLPSTTDSENCVSPMWEDEQNLHDVENIPQLNGREVDNRKSRNVPLTPVEKRKTLPARVQDHSTVFTPLNNQVNNSRNIKPDLSAKRLQFGATDNSATKLEKTVPHQSVGNGLIKPEPLKELKSSVKRTFEEVEKSPEQAEILCKKNDAAYKRSFQIPEKTSRAHTGLTGIFSIVGLDDVKSAPKCQQFNERTGPKQSSPIAVAKNLFCELEDQGEEGGKAEPNSSSSTSPGDERNIRRSLSLESDVSAHEMSLVANTPQKLSDAKQEVLSSSFEELDENEISAVTPMARPTVATPKHSSAKQRRGECERSLLDHPHGLSDSMIKSPGFLKPKNVVAFRSYCSSINRSCTSHLSLASFDAMEMSASASFHNAVTPVQKKRPSLNNSLYQTPQQMVVSHTPYRTPKSVRRGPERVEGAPILGTPDYLAPELLLGKPHDFMVDWWALGVCLFEFLTGVPPFNDETPQLVFQNILNRDIPWPDGEEELTPNSRNAIEILLTMDTLKRAGLKELKDHPFFDGVDWENLHHQTMPFIPQPDNETDTSYFEARNTAQHLTVSGFSL

Target ORF information:

RefSeq Version	NM_001003609.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase-like (mastl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003609.2

ORF Insert Sequence:

ATGGAAGCTC GTGGATTGGC ATGCGAAAGC TCAAACAGTG CAGTGAAAGC TCCTTCCATC 
GAGGACTTCG TGCTCGTCAA ACCCATCAGT CGAGGAGCTT TCGGGAAGGT TTACCTCGCC 
AGAAAGAAAT GCAACTCCAA ACTCTATGCC ATCAAGGTGG TGAAGAAAGC AGAAATGGTG 
GATAAGAACA TGACTGAGCA GATGAGAGCT GAGAGAGATG CTCTGGCTCT CAGTAAAAGC 
CCTTTCATTG TGCATCTGTT TTACTCCCTT CAAACCGCAA CTAAAGTCTA TCTGGTGATG 
GAGTATCTGA TTGGAGGAGA TGTGAAATCG CTTCTTCATA TTTATGGCTA CTTTGATGAG 
GACATGTCTC TGAAATACAT CTCAGAGGTC GCGCTCGCAC TGGATTACCT TCACAGACAC 
AGCATTATTC ACAGGGATTT AAAACCAGAC AACATGCTCA TCTCGAACGA GGGTCACATT 
AAGCTTACAG ACTTTGGCCT CTCCAAGGTT AAACTCGACA GAGAGCTAAA CCTGATGGAC 
ATTTTAACCA CACCATCTTT GGTGAAGCCT ACAAAGGATT ATTTTCGAAC ACCAGGTCAA 
GTTCTGTCAC TGATCAGCTC TCTTGGTCTT AACACCCCAG TTATAGAGGG TAAGCGTCAC 
AGCAGCACAG TACTGGGCAG CCCAATGTCC TGTGGGAAAG TCAAGCAGAG GAACAGGTCT 
CTGGGCTCAC CTCTCATGAA GAGACGGGCC GAGTACATGA ATTCCCCAGT GTGCACAAGT 
CGAGCTCTAG CATCCAACAG TGTGTTCAGT CCTGTCCTGC TGGCGAGGAG TTTGACACCA 
AGGCTGTTAA AGTCAGGGAA GAGACTCGAT ACTATGAGTG TTGGGAGCAC ACACTCATGC 
ATGCTACCAT CCACTACTGA CTCTGAAAAC TGCGTCAGCC CTATGTGGGA AGATGAGCAG 
AACTTGCATG ATGTCGAGAA CATTCCACAG CTGAATGGAA GAGAAGTTGA CAACAGAAAG 
TCAAGAAACG TTCCCTTGAC TCCTGTGGAA AAGAGGAAGA CGCTTCCAGC GCGAGTTCAG 
GATCACAGCA CTGTTTTTAC TCCCCTCAAT AACCAAGTTA ACAACAGCAG GAATATTAAA 
CCAGACCTTT CTGCAAAAAG GCTCCAGTTT GGTGCAACTG ACAACTCTGC CACTAAATTG 
GAGAAGACTG TTCCTCATCA GTCAGTTGGG AATGGTCTTA TAAAACCAGA GCCTCTAAAA 
GAGCTCAAAT CTTCAGTGAA GAGAACATTT GAGGAGGTTG AGAAAAGCCC AGAACAGGCT 
GAGATTTTAT GCAAGAAAAA TGACGCAGCG TACAAGAGAT CCTTCCAGAT TCCTGAGAAG 
ACCTCAAGGG CCCACACAGG CTTGACTGGA ATTTTCTCGA TTGTAGGGCT TGACGATGTT 
AAAAGTGCAC CTAAATGTCA ACAGTTCAAT GAGAGAACTG GCCCAAAGCA GTCTAGTCCC 
ATAGCTGTGG CCAAAAACCT CTTCTGTGAA CTGGAAGATC AGGGGGAAGA GGGGGGTAAA 
GCAGAGCCCA ACTCCAGCTC TTCAACCTCG CCCGGTGATG AGCGAAACAT CAGAAGAAGC 
CTAAGTTTAG AGTCTGACGT CTCAGCACAT GAGATGTCAC TGGTTGCTAA CACGCCACAA 
AAACTGTCAG ACGCCAAACA AGAAGTTTTA TCTTCGTCTT TTGAGGAGTT GGACGAAAAC 
GAGATTTCTG CAGTCACCCC GATGGCCAGA CCCACGGTTG CTACACCAAA GCATAGCAGC 
GCAAAGCAGC GCAGGGGTGA ATGTGAGCGT TCTCTGCTTG ATCATCCTCA TGGCCTGTCT 
GACAGTATGA TCAAATCACC CGGCTTCCTC AAACCCAAAA ATGTGGTTGC GTTTAGGAGC 
TACTGTAGCT CAATCAACCG CTCATGTACG TCACATCTCA GCCTGGCCTC GTTTGATGCC 
ATGGAAATGT CTGCGTCAGC GTCCTTTCAC AACGCTGTGA CTCCAGTTCA GAAGAAAAGG 
CCCAGTCTAA ACAACTCTCT TTACCAGACT CCCCAGCAGA TGGTTGTGTC TCACACTCCC 
TATAGGACAC CTAAAAGTGT CCGTAGAGGT CCTGAACGTG TTGAAGGGGC TCCTATTTTG 
GGTACTCCTG ACTACTTGGC ACCAGAACTT TTATTAGGCA AGCCTCATGA TTTCATGGTG 
GACTGGTGGG CATTGGGTGT TTGTCTGTTC GAGTTTCTCA CGGGAGTCCC ACCTTTCAAC 
GATGAGACCC CTCAATTGGT GTTTCAGAAC ATTCTCAACA GAGATATACC ATGGCCAGAC 
GGAGAAGAGG AGTTGACTCC AAATTCAAGA AATGCCATTG AGATTCTGCT CACCATGGAC 
ACACTCAAAC GGGCCGGCTT AAAAGAGTTG AAGGATCACC CGTTTTTTGA TGGTGTGGAC 
TGGGAAAACC TACACCATCA GACGATGCCC TTCATCCCAC AGCCTGACAA CGAGACTGAC 
ACATCGTACT TTGAGGCCAG GAATACGGCA CAACATCTGA CCGTTTCCGG CTTCAGTCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mastl ( XM_021470139.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325814.1 Danio rerio microtubule associated serine/threonine kinase-like (mastl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa151671
Clone ID Related Accession (Same CDS sequence)	XM_021470139.1
Accession Version	XM_021470139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase greatwall isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTC GTGGATTGGC ATGCGAAAGC TCAAACAGTG CAGTGAAAGC TCCTTCCATC 
GAGGACTTCG TGCTCGTCAA ACCCATCAGT CGAGGAGCTT TCGGGAAGGT TTACCTCGCC 
AGAAAGAAAT GCAACTCCAA ACTCTATGCC ATCAAGGTGG TGAAGAAAGC AGAAATGGTG 
GATAAGAACA TGACTGAGCA GATGAGAGCT GAGAGAGATG CTCTGGCTCT CAGTAAAAGC 
CCTTTCATTG TGCATCTGTT TTACTCCCTT CAAACCGCAA CTAAAGTCTA TCTGGTGATG 
GAGTATCTGA TTGGAGGAGA TGTGAAATCG CTTCTTCATA TTTATGGCTA CTTTGATGAG 
GACATGTCTC TGAAATACAT CTCAGAGGTC GCGCTCGCAC TGGATTACCT TCACAGACAC 
AGCATTATTC ACAGGGATTT AAAACCAGAC AACATGCTCA TCTCGAACGA GGGTCACATT 
AAGCTTACAG ACTTTGGCCT CTCCAAGGTT AAACTCGACA GAGAGCTAAA CCTGATGGAC 
ATTTTAACCA CACCATCTTT GGTGAAGCCT ACAAAGGATT ATTTTCGAAC ACCAGGTCAA 
GTTCTGTCAC TGATCAGCTC TCTTGGTCTT AACACCCCAG TTATAGAGGG TAAGCGTCAC 
AGCAGCACAG TACTGGGCAG CCCAATGTCC TGTGGGAAAG TCAAGCAGAG GAACAGGTCT 
CTGGGCTCAC CTCTCATGAA GAGACGGGCC GAGTACATGA ATTCCCCAGT GTGCACAAGT 
CGAGCTCTAG CATCCAACAG TGTGTTCAGT CCTGTCCTGC TGGCGAGGAG TTTGACACCA 
AGGCTGTTAA AGTCAGGGAA GAGACTCGAT ACTATGAGTG TTGGGAGCAC ACACTCATGC 
ATGCTACCAT CCACTACTGA CTCTGAAAAC TGCGTCAGCC CTATGTGGGA AGATGAGCAG 
AACTTGCATG ATGTCGAGAA CATTCCACAG CTGAATGGAA GAGAAGTTGA CAACAGAAAG 
TCAAGAAACG TTCCCTTGAC TCCTGTGGAA AAGAGGAAGA CGCTTCCAGC GCGAGTTCAG 
GATCACAGCA CTGTTTTTAC TCCCCTCAAT AACCAAGTTA ACAACAGCAG GAATATTAAA 
CCAGACCTTT CTGCAAAAAG GCTCCAGTTT GGTGCAACTG ACAACTCTGC CACTAAATTG 
GAGAAGACTG TTCCTCATCA GTCAGTTGGG AATGGTCTTA TAAAACCAGA GCCTCTAAAA 
GAGCTCAAAT CTTCAGTGAA GAGAACATTT GAGGAGGTTG AGAAAAGCCC AGAACAGGCT 
GAGATTTTAT GCAAGAAAAA TGACGCAGCG TACAAGAGAT CCTTCCAGAT TCCTGAGAAG 
ACCTCAAGGG CCCACACAGG CTTGACTGGA ATTTTCTCGA TTGTAGGGCT TGACGATGTT 
AAAAGTGCAC CTAAATGTCA ACAGTTCAAT GAGAGAACTG GCCCAAAGCA GTCTAGTCCC 
ATAGCTGTGG CCAAAAACCT CTTCTGTGAA CTGGAAGATC AGGGGGAAGA GGGGGGTAAA 
GCAGAGCCCA ACTCCAGCTC TTCAACCTCG CCCGGTGATG AGCGAAACAT CAGAAGAAGC 
CTAAGTTTAG AGTCTGACGT CTCAGCACAT GAGATGTCAC TGGTTGCTAA CACGCCACAA 
AAACTGTCAG ACGCCAAACA AGAAGTTTTA TCTTCGTCTT TTGAGGAGTT GGACGAAAAC 
GAGATTTCTG CAGTCACCCC GATGGCCAGA CCCACGGTTG CTACACCAAA GCATAGCAGC 
GCAAAGCAGC GCAGGGGTGA ATGTGAGCGT TCTCTGCTTG ATCATCCTCA TGGCCTGTCT 
GACAGTATGA TCAAATCACC CGGCTTCCTC AAACCCAAAA ATGTGGTTGC GTTTAGGAGC 
TACTGTAGCT CAATCAACCG CTCATGTACG TCACATCTCA GCCTGGCCTC GTTTGATGCC 
ATGGAAATGT CTGCGTCAGC GTCCTTTCAC AACGCTGTGA CTCCAGTTCA GAAGAAAAGG 
CCCAGTCTAA ACAACTCTCT TTACCAGACT CCCCAGCAGA TGGTTGTGTC TCACACTCCC 
TATAGGACAC CTAAAAGTGT CCGTAGAGGT CCTGAACGTG TTGAAGGGGC TCCTATTTTG 
GGTACTCCTG ACTACTTGGC ACCAGAACTT TTATTAGGCA AGCCTCATGT TTCAGGGCCT 
GTACGTTATG ATTTCATGGT GGACTGGTGG GCATTGGGTG TTTGTCTGTT CGAGTTTCTC 
ACGGGAGTCC CACCTTTCAA CGATGAGACC CCTCAATTGG TGTTTCAGAA CATTCTCAAC 
AGAGATATAC CATGGCCAGA CGGAGAAGAG GAGTTGACTC CAAATTCAAG AAATGCCATT 
GAGATTCTGC TCACCATGGA CACACTCAAA CGGGCCGGCT TAAAAGAGTT GAAGGATCAC 
CCGTTTTTTG ATGGTGTGGA CTGGGAAAAC CTACACCATC AGACGATGCC CTTCATCCCA 
CAGCCTGACA ACGAGACTGA CACATCGTAC TTTGAGGCCA GGAATACGGC ACAACATCTG 
ACCGTTTCCG GCTTCAGTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325814.1
CDS	486..3089
Translation	MEARGLACESSNSAVKAPSIEDFVLVKPISRGAFGKVYLARKKCNSKLYAIKVVKKAEMVDKNMTEQMRAERDALALSKSPFIVHLFYSLQTATKVYLVMEYLIGGDVKSLLHIYGYFDEDMSLKYISEVALALDYLHRHSIIHRDLKPDNMLISNEGHIKLTDFGLSKVKLDRELNLMDILTTPSLVKPTKDYFRTPGQVLSLISSLGLNTPVIEGKRHSSTVLGSPMSCGKVKQRNRSLGSPLMKRRAEYMNSPVCTSRALASNSVFSPVLLARSLTPRLLKSGKRLDTMSVGSTHSCMLPSTTDSENCVSPMWEDEQNLHDVENIPQLNGREVDNRKSRNVPLTPVEKRKTLPARVQDHSTVFTPLNNQVNNSRNIKPDLSAKRLQFGATDNSATKLEKTVPHQSVGNGLIKPEPLKELKSSVKRTFEEVEKSPEQAEILCKKNDAAYKRSFQIPEKTSRAHTGLTGIFSIVGLDDVKSAPKCQQFNERTGPKQSSPIAVAKNLFCELEDQGEEGGKAEPNSSSSTSPGDERNIRRSLSLESDVSAHEMSLVANTPQKLSDAKQEVLSSSFEELDENEISAVTPMARPTVATPKHSSAKQRRGECERSLLDHPHGLSDSMIKSPGFLKPKNVVAFRSYCSSINRSCTSHLSLASFDAMEMSASASFHNAVTPVQKKRPSLNNSLYQTPQQMVVSHTPYRTPKSVRRGPERVEGAPILGTPDYLAPELLLGKPHVSGPVRYDFMVDWWALGVCLFEFLTGVPPFNDETPQLVFQNILNRDIPWPDGEEELTPNSRNAIEILLTMDTLKRAGLKELKDHPFFDGVDWENLHHQTMPFIPQPDNETDTSYFEARNTAQHLTVSGFSL

Target ORF information:

RefSeq Version	XM_021470139.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated serine/threonine kinase-like (mastl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470139.1

ORF Insert Sequence:

ATGGAAGCTC GTGGATTGGC ATGCGAAAGC TCAAACAGTG CAGTGAAAGC TCCTTCCATC 
GAGGACTTCG TGCTCGTCAA ACCCATCAGT CGAGGAGCTT TCGGGAAGGT TTACCTCGCC 
AGAAAGAAAT GCAACTCCAA ACTCTATGCC ATCAAGGTGG TGAAGAAAGC AGAAATGGTG 
GATAAGAACA TGACTGAGCA GATGAGAGCT GAGAGAGATG CTCTGGCTCT CAGTAAAAGC 
CCTTTCATTG TGCATCTGTT TTACTCCCTT CAAACCGCAA CTAAAGTCTA TCTGGTGATG 
GAGTATCTGA TTGGAGGAGA TGTGAAATCG CTTCTTCATA TTTATGGCTA CTTTGATGAG 
GACATGTCTC TGAAATACAT CTCAGAGGTC GCGCTCGCAC TGGATTACCT TCACAGACAC 
AGCATTATTC ACAGGGATTT AAAACCAGAC AACATGCTCA TCTCGAACGA GGGTCACATT 
AAGCTTACAG ACTTTGGCCT CTCCAAGGTT AAACTCGACA GAGAGCTAAA CCTGATGGAC 
ATTTTAACCA CACCATCTTT GGTGAAGCCT ACAAAGGATT ATTTTCGAAC ACCAGGTCAA 
GTTCTGTCAC TGATCAGCTC TCTTGGTCTT AACACCCCAG TTATAGAGGG TAAGCGTCAC 
AGCAGCACAG TACTGGGCAG CCCAATGTCC TGTGGGAAAG TCAAGCAGAG GAACAGGTCT 
CTGGGCTCAC CTCTCATGAA GAGACGGGCC GAGTACATGA ATTCCCCAGT GTGCACAAGT 
CGAGCTCTAG CATCCAACAG TGTGTTCAGT CCTGTCCTGC TGGCGAGGAG TTTGACACCA 
AGGCTGTTAA AGTCAGGGAA GAGACTCGAT ACTATGAGTG TTGGGAGCAC ACACTCATGC 
ATGCTACCAT CCACTACTGA CTCTGAAAAC TGCGTCAGCC CTATGTGGGA AGATGAGCAG 
AACTTGCATG ATGTCGAGAA CATTCCACAG CTGAATGGAA GAGAAGTTGA CAACAGAAAG 
TCAAGAAACG TTCCCTTGAC TCCTGTGGAA AAGAGGAAGA CGCTTCCAGC GCGAGTTCAG 
GATCACAGCA CTGTTTTTAC TCCCCTCAAT AACCAAGTTA ACAACAGCAG GAATATTAAA 
CCAGACCTTT CTGCAAAAAG GCTCCAGTTT GGTGCAACTG ACAACTCTGC CACTAAATTG 
GAGAAGACTG TTCCTCATCA GTCAGTTGGG AATGGTCTTA TAAAACCAGA GCCTCTAAAA 
GAGCTCAAAT CTTCAGTGAA GAGAACATTT GAGGAGGTTG AGAAAAGCCC AGAACAGGCT 
GAGATTTTAT GCAAGAAAAA TGACGCAGCG TACAAGAGAT CCTTCCAGAT TCCTGAGAAG 
ACCTCAAGGG CCCACACAGG CTTGACTGGA ATTTTCTCGA TTGTAGGGCT TGACGATGTT 
AAAAGTGCAC CTAAATGTCA ACAGTTCAAT GAGAGAACTG GCCCAAAGCA GTCTAGTCCC 
ATAGCTGTGG CCAAAAACCT CTTCTGTGAA CTGGAAGATC AGGGGGAAGA GGGGGGTAAA 
GCAGAGCCCA ACTCCAGCTC TTCAACCTCG CCCGGTGATG AGCGAAACAT CAGAAGAAGC 
CTAAGTTTAG AGTCTGACGT CTCAGCACAT GAGATGTCAC TGGTTGCTAA CACGCCACAA 
AAACTGTCAG ACGCCAAACA AGAAGTTTTA TCTTCGTCTT TTGAGGAGTT GGACGAAAAC 
GAGATTTCTG CAGTCACCCC GATGGCCAGA CCCACGGTTG CTACACCAAA GCATAGCAGC 
GCAAAGCAGC GCAGGGGTGA ATGTGAGCGT TCTCTGCTTG ATCATCCTCA TGGCCTGTCT 
GACAGTATGA TCAAATCACC CGGCTTCCTC AAACCCAAAA ATGTGGTTGC GTTTAGGAGC 
TACTGTAGCT CAATCAACCG CTCATGTACG TCACATCTCA GCCTGGCCTC GTTTGATGCC 
ATGGAAATGT CTGCGTCAGC GTCCTTTCAC AACGCTGTGA CTCCAGTTCA GAAGAAAAGG 
CCCAGTCTAA ACAACTCTCT TTACCAGACT CCCCAGCAGA TGGTTGTGTC TCACACTCCC 
TATAGGACAC CTAAAAGTGT CCGTAGAGGT CCTGAACGTG TTGAAGGGGC TCCTATTTTG 
GGTACTCCTG ACTACTTGGC ACCAGAACTT TTATTAGGCA AGCCTCATGT TTCAGGGCCT 
GTACGTTATG ATTTCATGGT GGACTGGTGG GCATTGGGTG TTTGTCTGTT CGAGTTTCTC 
ACGGGAGTCC CACCTTTCAA CGATGAGACC CCTCAATTGG TGTTTCAGAA CATTCTCAAC 
AGAGATATAC CATGGCCAGA CGGAGAAGAG GAGTTGACTC CAAATTCAAG AAATGCCATT 
GAGATTCTGC TCACCATGGA CACACTCAAA CGGGCCGGCT TAAAAGAGTT GAAGGATCAC 
CCGTTTTTTG ATGGTGTGGA CTGGGAAAAC CTACACCATC AGACGATGCC CTTCATCCCA 
CAGCCTGACA ACGAGACTGA CACATCGTAC TTTGAGGCCA GGAATACGGC ACAACATCTG 
ACCGTTTCCG GCTTCAGTCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)
Briolat V,Jouneau L,Carvalho R,Palha N,Langevin C,Herbomel P,Schwartz O,Spaink HP,Levraud JP,Boudinot P

In vivo inactivation of MASTL kinase results in thrombocytopenia.
Experimental hematology37(8)901-8(2009 Aug)
Johnson HJ,Gandhi MJ,Shafizadeh E,Langer NB,Pierce EL,Paw BH,Gilligan DM,Drachman JG

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mastl cDNA ORF clone, Danio rerio(zebrafish)

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