slc12a2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc12a2
Entrez Gene ID	415170
Full Name	solute carrier family 12 (sodium/potassium/chloride transporter), member 2
Synonyms	slc12a4,zgc:85961
General protein information	Preferred Names solute carrier family 12 (sodium/potassium/chloride transporter), member 2 Names solute carrier family 12 member 2 little ears lte solute carrier family 12 (potassium/chloride transporters), member 2 solute carrier family 12 (potassium/chloride transporters), member 4 zfnkcc1 zmp:0000000537
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002080.1	NP_001002080.1	solute carrier family 12 member 2 isoform 1
NM_001163654.1	NP_001157126.1	solute carrier family 12 member 2 isoform 2
XM_021479152.1	XP_021334827.1	solute carrier family 12 member 2 isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	SLC12A2	XP_001098624.1
Rattus norvegicus (Norway rat)	Slc12a2	NP_113986.1
Danio rerio (zebrafish)	slc12a2	NP_001002080.1
Drosophila melanogaster (fruit fly)	Ncc69	NP_648572.1
Homo sapiens (human)	SLC12A2	NP_001243390.1
Bos taurus (cattle)	SLC12A2	NP_777207.1
Gallus gallus (chicken)	SLC12A2	XP_004949435.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001557	XP_321556.3
Pan troglodytes (chimpanzee)	SLC12A2	XP_003310846.1
Xenopus tropicalis (tropical clawed frog)	slc12a2	XP_004910531.1
Canis lupus familiaris (dog)	SLC12A2	XP_538611.5
Mus musculus (house mouse)	Slc12a2	NP_033220.2

Gene Ontology
Bibliography

Related articles in PubMed

Glycine is able to induce both a motility speed in- and decrease during zebrafish neuronal migration.
Theisen U, Hey S, Hennig CD, Schnabel R, Köster RW
Communicative & integrative biology11(3)1-7(2018)

In Vivo Characterization of an AHR-Dependent Long Noncoding RNA Required for Proper
Garcia GR, Goodale BC, Wiley MW, La Du JK, Hendrix DA, Tanguay RL
Molecular pharmacology91(6)609-619(2017 06)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Characterization of the γ-aminobutyric acid signaling system in the zebrafish (Danio rerio Hamilton) central nervous system by reverse transcription-quantitative polymerase chain reaction.
Cocco A, Rönnberg AM, Jin Z, André GI, Vossen LE, Bhandage AK, Thörnqvist PO, Birnir B, Winberg S
Neuroscience343300-321(2017 02)

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
Sullivan C, Lage CR, Yoder JA, Postlethwait JH, Kim CH
PloS one12(6)e0179517(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following slc12a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03681	NM_001163654.1 Latest version!	Danio rerio solute carrier family 12 member 2 (slc12a2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa03782	NM_001002080.1 Latest version!	Danio rerio solute carrier family 12 member 2 (slc12a2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa147601	XM_021479152.1 Latest version!	Danio rerio solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc12a2 ( NM_001163654.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001157126.1 Danio rerio solute carrier family 12 member 2 (slc12a2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa03681
Clone ID Related Accession (Same CDS sequence)	NM_001163654.1
Accession Version	NM_001163654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554566400000
Organism	Danio rerio(zebrafish)
Product	solute carrier family 12 member 2 isoform 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ259737.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GQ259737.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGCGT CACCTCCAAT CTCCGCAGGG GATTATCTCT CTGCGCCCGA ACCTGATGCG 
CTTAAACCCG CCGGTCCGAC TCCGAGCCAG AGCCGCTTCC AGGTGGATCT GGTGACAGAG 
TCCGCAGGGG ACGGTGAAAC CACTGTCGGC TTCGACTCAT CCCCACCCGA GTACGTTGCC 
GAGCCGCCGC CCGATGGGCT CCGGGATTCA GTATCTGGCG GCGAGGAAGC CAAGGGCCGG 
TTTCGGGTTG TGAACTTTGC GGCCTCAAGT CCGGACGCCG CGCCGGCGGA AACCGCGCAG 
AACGGGGACA CGGTGATGAG CGAGGGCAGT TTGCACTCCT CCACCGGCGG CCAGCAGCAC 
CATCACTACG ATACCCACAC CAACACCTAC TACCTCCGTA CGTTCGGACA CAACACCATC 
GACGCCGTGC CCAAGATCGA CTTCTACCGG CAGACCGCCG CTCCGCTGGG CGAGAAACTC 
ATTAGACCCA CCCTCTCAGA ACTGCACGAC GAGCTGGACA AGGAGCCATT TGAGGATGGG 
TTTGCTAACG GCGAGGAGTT GACCCCTGCC GAGGAGTCTG CAGCTAAAGA TGTATCAGAG 
TCTAAAGGAG TGGTGAAGTT CGGATGGATC AAGGGCGTGC TGGTGCGCTG CATGTTAAAC 
ATATGGGGTG TGATGCTCTT CATTCGAATG ACATGGATCG TGGGCCAGGC TGGAATTGCA 
TATTCCTGTA TCATTGTTAT TATGGCAACT GTTGTGACAA CCATCACAGG CTGCTCGACT 
TCGGCGATTG CAACTAATGG CTTTGTACGA GGAGGTGGAG CATATTACTT AATATCTCGA 
AGTCTGGGTC CAGAGTTTGG TGGCTCCATT GGTCTCATTT TTGCCTTTGC CAATGCTGTT 
GCTGTAGCCA TGTATGTTGT GGGTTTTGCC GAAACTGTTG TTGAGCTACT CATGGACTCT 
GATCTATTAA TGATCGATCA AACAAATGAC ATCAGAGTCA TTGGTACCAT TACAGTCATT 
CTGCTTCTCG GCATTTCAGT GGCTGGAATG GAGTGGGAAG CCAAGGCCCA GATATTCCTA 
TTGGTCATAT TGATTACTGC CATTTTCAAC TACTTCATTG GGTCCTTCAT TGCTGTTGAC 
TCGAAGAAGA AATTTGGCTT CTTTAGTTAT GATGCTGGCA TCTTGGCGGA AAATTTTGGT 
CCAGACTTTC GAGGACAGAC GTTTTTCTCT GTTTTTTCCA TCTTCTTCCC AGCAGCCACA 
GGCATTTTGG CTGGTGCCAA CATCTCAGGA GATCTAGCCG ACCCTCAAAT GGCTATCCCA 
AAAGGAACGC TTTTGGCTAT TCTCATAACA GGCCTTGTGT ACGTGGGTGT GGCCATTTCT 
GCAGGTGCTT GCATCGTGCG TGATGCTACA GGAATCGAAA GTAACTTTAC TCTCATTTCA 
AACTGTACTG ATGCAGCCTG TAAGTATGGC TATGACTTCA GTTCCTGCAG ACCTACGGTT 
GAAGGAGAAG TATCTTCCTG CAAGTTTGGT CTTCACAATG ATTTCCAGGT TATGAGCGTG 
GTGTCAGGCT TCAGTCCTCT TATCAGTGCT GGGATTTTCT CTGCCACACT TTCCTCTGCC 
CTGGCATCTC TCGTCAGTGC CCCAAAAGTG TTTCAGGCTT TATGCAAAGA CAATATCTAT 
CCTGGAATTG CAATTTTTGG AAAGGGCTAT GGCAAGAACA ACGAGCCCCT GCGAGGATAT 
TTTCTTACCT TTGGCATTGC GTTAGCTTTT ATTCTAATTG CGGAGTTGAA CGTCATTGCC 
CCAATCATTT CCAACTTTTT CCTGGCATCA TACGCTCTCA TCAACTTCTC AGTGTTTCAC 
GCTTCGCTGG CCAATTCCCC TGGATGGAGG CCAAGCTTCA AGTACTACAA CATGTGGGCG 
TCTCTGGCCG GGGCAATCCT ATGTTGTGTT GTCATGTTTA TCATCAATTG GTGGGCAGCG 
CTTTTGACCA ACGTCATTGT CTTATCCCTT TACATCTACG TCAGCTACAA AAAACCAGAT 
GTGAATTGGG GTTCGTCAAC TCAAGCTCTG ACGTATCATC AGGCCTTAAC ACACAGTTTA 
CAGCTGTGCG GAGTAGCTGA CCACATTAAA ACCTTCAGGC CACAGTGTCT GGTGATGACT 
GGGGCTCCAA ACTCGCGTCC AGCTATTCTT CATCTGGTCC ATGCCTTCAC CAAAAATGTG 
GGGCTGATGT TATGTGGGCA TGTGCGCATT AGTTCTCGAC GGCCCAACTT CAAAGAGCTA 
AACAGCGATA TGTTGCGTTA CCAGAGGTGG CTCCTTAACA ACAACTCCAA GGCCTTTTAC 
ACATGTGTCG TTGCTGAAGA CCTCCGTCAA GGAACCCAGT ACATGCTGCA GGCTGCTGGA 
CTGGGTAGAT TGAGGCCCAA TACTCTGGTT ATTGGTTTCA AAAATGACTG GCGCATTGGA 
GATATAAAGG AGGTTGAGAC CTACATAAAC CTAATACATG ATGCGTTTGA TTTTCAGTAT 
GGTGTTGTCA TTCTCAGACT GCGAGAGGGA CTTGATATTT CTCACATTCA AGGTCAAGAT 
GATTCTTCAG GTATGAAGGA TGTGGTGGTT TCAGTGGACA TAAGCAAAGA CTCGGATGGA 
GACTCATCCA AACCCTCCTC CAAGGCCACC AGTGTTCAGA ACAGCCCAGC GGTCCAGAAA 
GATAAAAAGA GCCCGACAGT TCCTCTAAAT GTAGCTGATC AGAGGTTGTT GGATGCCAGT 
CAACAGTTTC AGCAGAAACA GGGGAAGGGG ACAGTGGATG TGTGGTGGCT CTTTGATGAC 
GGGGGTCTGA CTCTACTGAT CCCCTATCTT ATCGCCAACA AGAAGAAATG GAAGGACTGT 
AAGATCCGCG TGTTCATCGG TGGGAAGATC AACAGAATAG ACCATGACCG CAGAGCCATG 
GCCACCTTGC TCAGCAAGTT CAGGATCGAT TTCTCTGACA TCACAGTCCT TGGTGACATC 
AACACAAAGC CAAAGTCTGA GGGTTTGACA GAGTTTGCTG AAATGATCGA GCCATATAAA 
CTGAGAGAAG ATGACATGGA GCAGGAAGCT GCTGAGAAGC TGAAAAGCGA GGAACCCTGG 
AGGATCACTG ACAATGAGCT GGAGCTCTAC AAAGCCAAGG GTAACCGTCA GATCAGACTG 
AATGAGCTGT TGAAGGAGCA CTCAAGCACA GCCAACCTCA TTGTCATGAG TATGCCGCTG 
GCCAGGAAAG GAGCCGTCTC CAGTGCCCTT TATATGGCCT GGCTAGACAC ACTATCCAAA 
GACCTGCCGC CCATCCTCTT AGTGCGCGGG AACCATCAGA GCGTCCTCAC GTTCTACTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157126.1
CDS	7..3369
Translation	MSASPPISAGDYLSAPEPDALKPAGPTPSQSRFQVDLVTESAGDGETTVGFDSSPPEYVAEPPPDGLRDSVSGGEEAKGRFRVVNFAASSPDAAPAETAQNGDTVMSEGSLHSSTGGQQHHHYDTHTNTYYLRTFGHNTIDAVPKIDFYRQTAAPLGEKLIRPTLSELHDELDKEPFEDGFANGEELTPAEESAAKDVSESKGVVKFGWIKGVLVRCMLNIWGVMLFIRMTWIVGQAGIAYSCIIVIMATVVTTITGCSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVELLMDSDLLMIDQTNDIRVIGTITVILLLGISVAGMEWEAKAQIFLLVILITAIFNYFIGSFIAVDSKKKFGFFSYDAGILAENFGPDFRGQTFFSVFSIFFPAATGILAGANISGDLADPQMAIPKGTLLAILITGLVYVGVAISAGACIVRDATGIESNFTLISNCTDAACKYGYDFSSCRPTVEGEVSSCKFGLHNDFQVMSVVSGFSPLISAGIFSATLSSALASLVSAPKVFQALCKDNIYPGIAIFGKGYGKNNEPLRGYFLTFGIALAFILIAELNVIAPIISNFFLASYALINFSVFHASLANSPGWRPSFKYYNMWASLAGAILCCVVMFIINWWAALLTNVIVLSLYIYVSYKKPDVNWGSSTQALTYHQALTHSLQLCGVADHIKTFRPQCLVMTGAPNSRPAILHLVHAFTKNVGLMLCGHVRISSRRPNFKELNSDMLRYQRWLLNNNSKAFYTCVVAEDLRQGTQYMLQAAGLGRLRPNTLVIGFKNDWRIGDIKEVETYINLIHDAFDFQYGVVILRLREGLDISHIQGQDDSSGMKDVVVSVDISKDSDGDSSKPSSKATSVQNSPAVQKDKKSPTVPLNVADQRLLDASQQFQQKQGKGTVDVWWLFDDGGLTLLIPYLIANKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDITVLGDINTKPKSEGLTEFAEMIEPYKLREDDMEQEAAEKLKSEEPWRITDNELELYKAKGNRQIRLNELLKEHSSTANLIVMSMPLARKGAVSSALYMAWLDTLSKDLPPILLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	NM_001163654.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 12 member 2 (slc12a2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163654.1

ORF Insert Sequence:

ATGTCGGCGT CACCTCCAAT CTCCGCAGGG GATTATCTCT CTGCGCCCGA ACCTGATGCG 
CTTAAACCCG CCGGTCCGAC TCCGAGCCAG AGCCGCTTCC AGGTGGATCT GGTGACAGAG 
TCCGCAGGGG ACGGTGAAAC CACTGTCGGC TTCGACTCAT CCCCACCCGA GTACGTTGCC 
GAGCCGCCGC CCGATGGGCT CCGGGATTCA GTATCTGGCG GCGAGGAAGC CAAGGGCCGG 
TTTCGGGTTG TGAACTTTGC GGCCTCAAGT CCGGACGCCG CGCCGGCGGA AACCGCGCAG 
AACGGGGACA CGGTGATGAG CGAGGGCAGT TTGCACTCCT CCACCGGCGG CCAGCAGCAC 
CATCACTACG ATACCCACAC CAACACCTAC TACCTCCGTA CGTTCGGACA CAACACCATC 
GACGCCGTGC CCAAGATCGA CTTCTACCGG CAGACCGCCG CTCCGCTGGG CGAGAAACTC 
ATTAGACCCA CCCTCTCAGA ACTGCACGAC GAGCTGGACA AGGAGCCATT TGAGGATGGG 
TTTGCTAACG GCGAGGAGTT GACCCCTGCC GAGGAGTCTG CAGCTAAAGA TGTATCAGAG 
TCTAAAGGAG TGGTGAAGTT CGGATGGATC AAGGGCGTGC TGGTGCGCTG CATGTTAAAC 
ATATGGGGTG TGATGCTCTT CATTCGAATG ACATGGATCG TGGGCCAGGC TGGAATTGCA 
TATTCCTGTA TCATTGTTAT TATGGCAACT GTTGTGACAA CCATCACAGG CTGCTCGACT 
TCGGCGATTG CAACTAATGG CTTTGTACGA GGAGGTGGAG CATATTACTT AATATCTCGA 
AGTCTGGGTC CAGAGTTTGG TGGCTCCATT GGTCTCATTT TTGCCTTTGC CAATGCTGTT 
GCTGTAGCCA TGTATGTTGT GGGTTTTGCC GAAACTGTTG TTGAGCTACT CATGGACTCT 
GATCTATTAA TGATCGATCA AACAAATGAC ATCAGAGTCA TTGGTACCAT TACAGTCATT 
CTGCTTCTCG GCATTTCAGT GGCTGGAATG GAGTGGGAAG CCAAGGCCCA GATATTCCTA 
TTGGTCATAT TGATTACTGC CATTTTCAAC TACTTCATTG GGTCCTTCAT TGCTGTTGAC 
TCGAAGAAGA AATTTGGCTT CTTTAGTTAT GATGCTGGCA TCTTGGCGGA AAATTTTGGT 
CCAGACTTTC GAGGACAGAC GTTTTTCTCT GTTTTTTCCA TCTTCTTCCC AGCAGCCACA 
GGCATTTTGG CTGGTGCCAA CATCTCAGGA GATCTAGCCG ACCCTCAAAT GGCTATCCCA 
AAAGGAACGC TTTTGGCTAT TCTCATAACA GGCCTTGTGT ACGTGGGTGT GGCCATTTCT 
GCAGGTGCTT GCATCGTGCG TGATGCTACA GGAATCGAAA GTAACTTTAC TCTCATTTCA 
AACTGTACTG ATGCAGCCTG TAAGTATGGC TATGACTTCA GTTCCTGCAG ACCTACGGTT 
GAAGGAGAAG TATCTTCCTG CAAGTTTGGT CTTCACAATG ATTTCCAGGT TATGAGCGTG 
GTGTCAGGCT TCAGTCCTCT TATCAGTGCT GGGATTTTCT CTGCCACACT TTCCTCTGCC 
CTGGCATCTC TCGTCAGTGC CCCAAAAGTG TTTCAGGCTT TATGCAAAGA CAATATCTAT 
CCTGGAATTG CAATTTTTGG AAAGGGCTAT GGCAAGAACA ACGAGCCCCT GCGAGGATAT 
TTTCTTACCT TTGGCATTGC GTTAGCTTTT ATTCTAATTG CGGAGTTGAA CGTCATTGCC 
CCAATCATTT CCAACTTTTT CCTGGCATCA TACGCTCTCA TCAACTTCTC AGTGTTTCAC 
GCTTCGCTGG CCAATTCCCC TGGATGGAGG CCAAGCTTCA AGTACTACAA CATGTGGGCG 
TCTCTGGCCG GGGCAATCCT ATGTTGTGTT GTCATGTTTA TCATCAATTG GTGGGCAGCG 
CTTTTGACCA ACGTCATTGT CTTATCCCTT TACATCTACG TCAGCTACAA AAAACCAGAT 
GTGAATTGGG GTTCGTCAAC TCAAGCTCTG ACGTATCATC AGGCCTTAAC ACACAGTTTA 
CAGCTGTGCG GAGTAGCTGA CCACATTAAA ACCTTCAGGC CACAGTGTCT GGTGATGACT 
GGGGCTCCAA ACTCGCGTCC AGCTATTCTT CATCTGGTCC ATGCCTTCAC CAAAAATGTG 
GGGCTGATGT TATGTGGGCA TGTGCGCATT AGTTCTCGAC GGCCCAACTT CAAAGAGCTA 
AACAGCGATA TGTTGCGTTA CCAGAGGTGG CTCCTTAACA ACAACTCCAA GGCCTTTTAC 
ACATGTGTCG TTGCTGAAGA CCTCCGTCAA GGAACCCAGT ACATGCTGCA GGCTGCTGGA 
CTGGGTAGAT TGAGGCCCAA TACTCTGGTT ATTGGTTTCA AAAATGACTG GCGCATTGGA 
GATATAAAGG AGGTTGAGAC CTACATAAAC CTAATACATG ATGCGTTTGA TTTTCAGTAT 
GGTGTTGTCA TTCTCAGACT GCGAGAGGGA CTTGATATTT CTCACATTCA AGGTCAAGAT 
GATTCTTCAG GTATGAAGGA TGTGGTGGTT TCAGTGGACA TAAGCAAAGA CTCGGATGGA 
GACTCATCCA AACCCTCCTC CAAGGCCACC AGTGTTCAGA ACAGCCCAGC GGTCCAGAAA 
GATAAAAAGA GCCCGACAGT TCCTCTAAAT GTAGCTGATC AGAGGTTGTT GGATGCCAGT 
CAACAGTTTC AGCAGAAACA GGGGAAGGGG ACAGTGGATG TGTGGTGGCT CTTTGATGAC 
GGGGGTCTGA CTCTACTGAT CCCCTATCTT ATCGCCAACA AGAAGAAATG GAAGGACTGT 
AAGATCCGCG TGTTCATCGG TGGGAAGATC AACAGAATAG ACCATGACCG CAGAGCCATG 
GCCACCTTGC TCAGCAAGTT CAGGATCGAT TTCTCTGACA TCACAGTCCT TGGTGACATC 
AACACAAAGC CAAAGTCTGA GGGTTTGACA GAGTTTGCTG AAATGATCGA GCCATATAAA 
CTGAGAGAAG ATGACATGGA GCAGGAAGCT GCTGAGAAGC TGAAAAGCGA GGAACCCTGG 
AGGATCACTG ACAATGAGCT GGAGCTCTAC AAAGCCAAGG GTAACCGTCA GATCAGACTG 
AATGAGCTGT TGAAGGAGCA CTCAAGCACA GCCAACCTCA TTGTCATGAG TATGCCGCTG 
GCCAGGAAAG GAGCCGTCTC CAGTGCCCTT TATATGGCCT GGCTAGACAC ACTATCCAAA 
GACCTGCCGC CCATCCTCTT AGTGCGCGGG AACCATCAGA GCGTCCTCAC GTTCTACTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a2 ( NM_001002080.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002080.1 Danio rerio solute carrier family 12 member 2 (slc12a2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa03782
Clone ID Related Accession (Same CDS sequence)	NM_001002080.1
Accession Version	NM_001002080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554566400000
Organism	Danio rerio(zebrafish)
Product	solute carrier family 12 member 2 isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC071283.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071283.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGCGT CACCTCCAAT CTCCGCGGGC GATTATCTCT CTGCGCCCGA ACCTGATGCG 
CTTAAACCCG CCGGTCCGAC TCCGAGCCAG AGCCGCTTCC AGGTGGATCT GGTGACAGAG 
TCCGCAGGGG ACGGTGAAAC CACTGTCGGC TTCGACTCAT CCCCACCCGA GTACGTTGCC 
GAGCCGCCGC CCGATGGGCT CCGGGATTCA GTATCTGGCG GCGAGAAAGC CAAGGGCCGG 
TTTCGGGTTG TGAACTTTGC GGCCTCAAGT CCGGACGCCG CGCCGGCGGA AACCGCGCAG 
AACGGGGACA CGGTGATGAG CGAGGGCAGT TTGCACTCCT CCACCGGCGG CCAGCAGCAC 
CATCACTACG ATACCCACAC CAACACCTAC TACCTCCGTA CGTTCGGACA CAACACCATC 
GACGCCGTGC CCAAGATCGA CTTCTACCGG CAGACCGCCG CTCCGCTGGG CGAGAAACTC 
ATTAGACCCA CCCTCTCAGA ACTGCACGAC GAGCTGGACA AGGAGCCATT TGAGGATGGG 
TTTGCTAACG GCGAGGAGTT GACCCCTGCC GAGGAGTCTG CAGCTAAAGA TGTATCAGAG 
TCTAAAGGAG TGGTGAAGTT CGGATGGATC AAGGGCGTGC TGGTGCGCTG CATGTTAAAC 
ATATGGGGTG TGATGCTCTT CATTCGAATG ACATGGATCG TGGGCCAAGC TGGAATTGCA 
TATTCCTGTA TCATTGTTAT TATGGCTACT GTTGTGACAA CCATCACAGG CTGCTCGACT 
TCGGCGATTG CAACTAATGG CTTTGTACGA GGAGGTGGAG CATATTACTT AATATCTCGA 
AGTCTGGGTC CAGAGTTTGG TGGCTCCATT GGTCTCATTT TTGCCTTTGC CAATGCTGTT 
GCTGTAGCCA TGTATGTTGT GGGTTTTGCC GAAACTGTTG TTGAGCTACT CATGGACTCT 
GGTCTATTAA TGATCGATCA AACAAATGAC ATCAGAGTCA TTGGGACCAT TACAGTCATT 
CTGCTTCTCG GCATTTCAGT GGCTGGAATG GAGTGGGAAG CCAAGGCCCA GATATTCCTA 
TTGGTCATAT TGATTACTGC CATTTTCAAC TACTTCATTG GGTCCTTCAT TGCTGTTGAC 
TCGAAGAAGA AATTTGGCTT CTTTAGTTAT GATGCTGGCA TCTTGGCGGA AAATTTTGGT 
CCAGACTTTC GAGGACAGAC GTTTTTCTCT GTTTTTTCCA TCTTCTTCCC AGCAGCCACA 
GGCATTTTGG CTGGTGCCAA CATCTCAGGA GATCTAGCAG ACCCTCAAAT GGCCATCCCA 
AAAGGAACGC TTTTGGCTAT TCTCATAACA GGCCTTGTGT ACGTGGGTGT GGCCATTTCT 
GCAGGTGCTT GCATCGTGCG TGATGCTACA GGAATCGAGA GTAACTTTAC TCTCATTTCA 
AACTGTACTG ATGCAGCCTG TAAGTATGGC TATGACTTCA GTTCCTGCAG ACCTACGGTT 
GAAGGAGAAG TATCTTCCTG CAAGTTTGGT CTTCACAATG ATTTCCAGGT TATGAGTGTG 
GTGTCAGGCT TCAGTCCTCT TATCAGTGCT GGGATTTTCT CTGCCACACT TTCCTCTGCC 
CTGGCATCTC TCGTCAGTGC CCCAAAAGTG TTTCAGGCTT TATGCAAAGA CAATATCTAT 
CCTGGAATTG CAATTTTTGG AAAGGGCTAT GGCAAGAACA ACGAGCCCCT GCGAGGATAT 
TTTCTTACCT TTGGCATTGC GTTAGCTTTT ATTCTAATTG CGGAGTTGAA CGTCATTGCC 
CCAATCATTT CCAACTTTTT CCTGGCATCA TACGCTCTCA TCAACTTCTC GGTGTTTCAC 
GCTTCGCTGG CCAATTCCCC TGGATGGAGG CCAAGCTTCA AGTACTACAA CATGTGGGCG 
TCTCTGGCCG GGGCAATCCT ATGTTGTGTT GTCATGTTTA TCATCAATTG GTGGGCAGCG 
CTTTTGACCA ACGTCATTGT CTTATCCCTT TACATCTACG TCAGCTACAA AAAACCAGAT 
GTGAATTGGG GTTCGTCAAC TCAAGCTCTG ACGTATCATC AGGCCTTAAC ACACAGTTTA 
CAGCTGTGCG GAGTAGCTGA CCACATTAAA ACCTTCAGGC CACAGTGTCT GGTGATGACT 
GGGGCTCCAA ACTCGCGTCC AGCTATTCTT CATCTGGTCC ATGCCTTCAC CAAAAATGTG 
GGGCTGATGT TATGTGGGCA TGTGCGCATT AGTTCTCGAC GGCCCAACTT CAAAGAGCTA 
AACAGCGATA TGTTGCGTTA CCAGAGGTGG CTCCTTAACA ACAACTCCAA GGCCTTTTAC 
ACATGTGTCG TTGCTGAAGA CCTCCGTCAA GGAACCCAGT ACATGCTGCA GGCTGCTGGA 
CTGGGTAGAT TGAGGCCCAA TACTCTGGTT ATTGGTTTCA AAAATGACTG GCGCATTGGA 
GACATAAAGG AGGTTGAGAC CTACATAAAC CTAATACATG ATGCGTTTGA TTTTCAGTAT 
GGTGTTGTCA TTCTCAGACT GCGAGAGGGA CTTGATATTT CTCACATTCA AGGTCAAGAT 
GATTCTTCAG GTATGAAGGA TGTGGTGGTT TCAGTGGACA TAAGCAAAGA CTCGGATGGA 
GACTCATCCA AACCCTCCTC CAAGGCCACC AGTGTTCAGA ACAGCCCAGC GGTCCAGAAA 
GATGAGGATG ATGATGGCAA AGCCCACACT CAGCCGCTGT TGAAGAAAGA TAAAAAGAGC 
CCGACAGTTC CTCTAAATGT AGCTGATCAG AGGTTGTTGG ATGCCAGTCA ACAGTTTCAG 
CAGAAACAGG GGAAGGGGAC AGTGGATGTG TGGTGGCTCT TTGATGACGG GGGTCTGACT 
CTACTGATCC CCTATCTTAT CGCCAACAAG AAGAAATGGA AGGACTGTAA GATCCGCGTG 
TTCATCGGTG GGAAGATCAA CAGAATAGAC CATGACCGCA GAGCCATGGC CACCTTGCTC 
AGCAAGTTCA GGATCGATTT CTCTGACATC ACAGTCCTTG GTGACATCAA CACAAAGCCA 
AAGTCTGAGG GTTTGACAGA GTTTGCTGAA ATGATCGAGC CATATAAACT GAGAGAAGAT 
GACATGGAGC AGGAAGCTGC TGAGAAGCTG AAAAGCGAGG AACCCTGGAG GATCACTGAC 
AATGAGCTGG AGCTCTACAA AGCCAAGGGT AACCGTCAGA TCAGACTGAA TGAGCTGTTG 
AAGGAGCACT CAAGCACAGC CAACCTCATT GTCATGAGTA TGCCGCTGGC CAGGAAAGGA 
GCCGTCTCCA GTGCCCTTTA TATGGCCTGG CTAGACACAC TATCCAAAGA CCTGCCGCCC 
ATCCTCTTAG TGCGCGGGAA CCATCAGAGC GTCCTCACGT TCTACTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002080.1
CDS	67..3477
Translation	MSASPPISAGDYLSAPEPDALKPAGPTPSQSRFQVDLVTESAGDGETTVGFDSSPPEYVAEPPPDGLRDSVSGGEKAKGRFRVVNFAASSPDAAPAETAQNGDTVMSEGSLHSSTGGQQHHHYDTHTNTYYLRTFGHNTIDAVPKIDFYRQTAAPLGEKLIRPTLSELHDELDKEPFEDGFANGEELTPAEESAAKDVSESKGVVKFGWIKGVLVRCMLNIWGVMLFIRMTWIVGQAGIAYSCIIVIMATVVTTITGCSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVELLMDSGLLMIDQTNDIRVIGTITVILLLGISVAGMEWEAKAQIFLLVILITAIFNYFIGSFIAVDSKKKFGFFSYDAGILAENFGPDFRGQTFFSVFSIFFPAATGILAGANISGDLADPQMAIPKGTLLAILITGLVYVGVAISAGACIVRDATGIESNFTLISNCTDAACKYGYDFSSCRPTVEGEVSSCKFGLHNDFQVMSVVSGFSPLISAGIFSATLSSALASLVSAPKVFQALCKDNIYPGIAIFGKGYGKNNEPLRGYFLTFGIALAFILIAELNVIAPIISNFFLASYALINFSVFHASLANSPGWRPSFKYYNMWASLAGAILCCVVMFIINWWAALLTNVIVLSLYIYVSYKKPDVNWGSSTQALTYHQALTHSLQLCGVADHIKTFRPQCLVMTGAPNSRPAILHLVHAFTKNVGLMLCGHVRISSRRPNFKELNSDMLRYQRWLLNNNSKAFYTCVVAEDLRQGTQYMLQAAGLGRLRPNTLVIGFKNDWRIGDIKEVETYINLIHDAFDFQYGVVILRLREGLDISHIQGQDDSSGMKDVVVSVDISKDSDGDSSKPSSKATSVQNSPAVQKDEDDDGKAHTQPLLKKDKKSPTVPLNVADQRLLDASQQFQQKQGKGTVDVWWLFDDGGLTLLIPYLIANKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDITVLGDINTKPKSEGLTEFAEMIEPYKLREDDMEQEAAEKLKSEEPWRITDNELELYKAKGNRQIRLNELLKEHSSTANLIVMSMPLARKGAVSSALYMAWLDTLSKDLPPILLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	NM_001002080.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 12 member 2 (slc12a2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002080.1

ORF Insert Sequence:

ATGTCGGCGT CACCTCCAAT CTCCGCGGGC GATTATCTCT CTGCGCCCGA ACCTGATGCG 
CTTAAACCCG CCGGTCCGAC TCCGAGCCAG AGCCGCTTCC AGGTGGATCT GGTGACAGAG 
TCCGCAGGGG ACGGTGAAAC CACTGTCGGC TTCGACTCAT CCCCACCCGA GTACGTTGCC 
GAGCCGCCGC CCGATGGGCT CCGGGATTCA GTATCTGGCG GCGAGAAAGC CAAGGGCCGG 
TTTCGGGTTG TGAACTTTGC GGCCTCAAGT CCGGACGCCG CGCCGGCGGA AACCGCGCAG 
AACGGGGACA CGGTGATGAG CGAGGGCAGT TTGCACTCCT CCACCGGCGG CCAGCAGCAC 
CATCACTACG ATACCCACAC CAACACCTAC TACCTCCGTA CGTTCGGACA CAACACCATC 
GACGCCGTGC CCAAGATCGA CTTCTACCGG CAGACCGCCG CTCCGCTGGG CGAGAAACTC 
ATTAGACCCA CCCTCTCAGA ACTGCACGAC GAGCTGGACA AGGAGCCATT TGAGGATGGG 
TTTGCTAACG GCGAGGAGTT GACCCCTGCC GAGGAGTCTG CAGCTAAAGA TGTATCAGAG 
TCTAAAGGAG TGGTGAAGTT CGGATGGATC AAGGGCGTGC TGGTGCGCTG CATGTTAAAC 
ATATGGGGTG TGATGCTCTT CATTCGAATG ACATGGATCG TGGGCCAAGC TGGAATTGCA 
TATTCCTGTA TCATTGTTAT TATGGCTACT GTTGTGACAA CCATCACAGG CTGCTCGACT 
TCGGCGATTG CAACTAATGG CTTTGTACGA GGAGGTGGAG CATATTACTT AATATCTCGA 
AGTCTGGGTC CAGAGTTTGG TGGCTCCATT GGTCTCATTT TTGCCTTTGC CAATGCTGTT 
GCTGTAGCCA TGTATGTTGT GGGTTTTGCC GAAACTGTTG TTGAGCTACT CATGGACTCT 
GGTCTATTAA TGATCGATCA AACAAATGAC ATCAGAGTCA TTGGGACCAT TACAGTCATT 
CTGCTTCTCG GCATTTCAGT GGCTGGAATG GAGTGGGAAG CCAAGGCCCA GATATTCCTA 
TTGGTCATAT TGATTACTGC CATTTTCAAC TACTTCATTG GGTCCTTCAT TGCTGTTGAC 
TCGAAGAAGA AATTTGGCTT CTTTAGTTAT GATGCTGGCA TCTTGGCGGA AAATTTTGGT 
CCAGACTTTC GAGGACAGAC GTTTTTCTCT GTTTTTTCCA TCTTCTTCCC AGCAGCCACA 
GGCATTTTGG CTGGTGCCAA CATCTCAGGA GATCTAGCAG ACCCTCAAAT GGCCATCCCA 
AAAGGAACGC TTTTGGCTAT TCTCATAACA GGCCTTGTGT ACGTGGGTGT GGCCATTTCT 
GCAGGTGCTT GCATCGTGCG TGATGCTACA GGAATCGAGA GTAACTTTAC TCTCATTTCA 
AACTGTACTG ATGCAGCCTG TAAGTATGGC TATGACTTCA GTTCCTGCAG ACCTACGGTT 
GAAGGAGAAG TATCTTCCTG CAAGTTTGGT CTTCACAATG ATTTCCAGGT TATGAGTGTG 
GTGTCAGGCT TCAGTCCTCT TATCAGTGCT GGGATTTTCT CTGCCACACT TTCCTCTGCC 
CTGGCATCTC TCGTCAGTGC CCCAAAAGTG TTTCAGGCTT TATGCAAAGA CAATATCTAT 
CCTGGAATTG CAATTTTTGG AAAGGGCTAT GGCAAGAACA ACGAGCCCCT GCGAGGATAT 
TTTCTTACCT TTGGCATTGC GTTAGCTTTT ATTCTAATTG CGGAGTTGAA CGTCATTGCC 
CCAATCATTT CCAACTTTTT CCTGGCATCA TACGCTCTCA TCAACTTCTC GGTGTTTCAC 
GCTTCGCTGG CCAATTCCCC TGGATGGAGG CCAAGCTTCA AGTACTACAA CATGTGGGCG 
TCTCTGGCCG GGGCAATCCT ATGTTGTGTT GTCATGTTTA TCATCAATTG GTGGGCAGCG 
CTTTTGACCA ACGTCATTGT CTTATCCCTT TACATCTACG TCAGCTACAA AAAACCAGAT 
GTGAATTGGG GTTCGTCAAC TCAAGCTCTG ACGTATCATC AGGCCTTAAC ACACAGTTTA 
CAGCTGTGCG GAGTAGCTGA CCACATTAAA ACCTTCAGGC CACAGTGTCT GGTGATGACT 
GGGGCTCCAA ACTCGCGTCC AGCTATTCTT CATCTGGTCC ATGCCTTCAC CAAAAATGTG 
GGGCTGATGT TATGTGGGCA TGTGCGCATT AGTTCTCGAC GGCCCAACTT CAAAGAGCTA 
AACAGCGATA TGTTGCGTTA CCAGAGGTGG CTCCTTAACA ACAACTCCAA GGCCTTTTAC 
ACATGTGTCG TTGCTGAAGA CCTCCGTCAA GGAACCCAGT ACATGCTGCA GGCTGCTGGA 
CTGGGTAGAT TGAGGCCCAA TACTCTGGTT ATTGGTTTCA AAAATGACTG GCGCATTGGA 
GACATAAAGG AGGTTGAGAC CTACATAAAC CTAATACATG ATGCGTTTGA TTTTCAGTAT 
GGTGTTGTCA TTCTCAGACT GCGAGAGGGA CTTGATATTT CTCACATTCA AGGTCAAGAT 
GATTCTTCAG GTATGAAGGA TGTGGTGGTT TCAGTGGACA TAAGCAAAGA CTCGGATGGA 
GACTCATCCA AACCCTCCTC CAAGGCCACC AGTGTTCAGA ACAGCCCAGC GGTCCAGAAA 
GATGAGGATG ATGATGGCAA AGCCCACACT CAGCCGCTGT TGAAGAAAGA TAAAAAGAGC 
CCGACAGTTC CTCTAAATGT AGCTGATCAG AGGTTGTTGG ATGCCAGTCA ACAGTTTCAG 
CAGAAACAGG GGAAGGGGAC AGTGGATGTG TGGTGGCTCT TTGATGACGG GGGTCTGACT 
CTACTGATCC CCTATCTTAT CGCCAACAAG AAGAAATGGA AGGACTGTAA GATCCGCGTG 
TTCATCGGTG GGAAGATCAA CAGAATAGAC CATGACCGCA GAGCCATGGC CACCTTGCTC 
AGCAAGTTCA GGATCGATTT CTCTGACATC ACAGTCCTTG GTGACATCAA CACAAAGCCA 
AAGTCTGAGG GTTTGACAGA GTTTGCTGAA ATGATCGAGC CATATAAACT GAGAGAAGAT 
GACATGGAGC AGGAAGCTGC TGAGAAGCTG AAAAGCGAGG AACCCTGGAG GATCACTGAC 
AATGAGCTGG AGCTCTACAA AGCCAAGGGT AACCGTCAGA TCAGACTGAA TGAGCTGTTG 
AAGGAGCACT CAAGCACAGC CAACCTCATT GTCATGAGTA TGCCGCTGGC CAGGAAAGGA 
GCCGTCTCCA GTGCCCTTTA TATGGCCTGG CTAGACACAC TATCCAAAGA CCTGCCGCCC 
ATCCTCTTAG TGCGCGGGAA CCATCAGAGC GTCCTCACGT TCTACTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a2 ( XM_021479152.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334827.1 Danio rerio solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X1, mRNA.

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CloneID	ODa147601
Clone ID Related Accession (Same CDS sequence)	XM_021479152.1
Accession Version	XM_021479152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	solute carrier family 12 member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGT CACCTCCAAT CTCCGCGGGC GATTATCTCT CTGCGCCCGA ACCTGATGCG 
CTTAAACCCG CCGGTCCGAC TCCGAGCCAG AGCCGCTTCC AGGTGGATCT GGTGACAGAG 
TCCGCAGGGG ACGGTGAAAC CACTGTCGGC TTCGACTCAT CCCCACCCGA GTACGTTGCC 
GAGCCGCCGC CCGATGGGCT CCGGGATTCA GTATCTGGCG GCGAGGAAGC CAAGGGCCGG 
TTTCGGGTTG TGAACTTTGC GGCCTCAAGT CCGGACGCCG CGCCGGCGGA AACCGCGCAG 
AACGGGGACA CGGTGATGAG CGAGGGCAGT TTGCACTCCT CCACCGGCGG CCAGCAGCAC 
CATCACTACG ATACACACAC CAACACCTAC TACCTCCGTA CGTTCGGACA CAACACCATC 
GACGCCGTGC CCAAGATCGA CTTCTACCGG CAGACCGCCG CTCCGCTGGG CGAGAAACTC 
ATTAGACCCA CCCTCTCAGA ACTGCACGAC GAGCTGGACA AGGAGCCATT TGAGGATGGG 
TTTGCTAACG GCGAGGAGTT GACCCCTGCC GAGGAGTCTG CAGCTAAAGA TGTATCAGAG 
TCTAAAGGAG TGGTGAAGTT CGGATGGATC AAGGGCGTGC TGGTGCGCTG CATGTTAAAC 
ATATGGGGTG TGATGCTCTT CATTCGAATG ACATGGATCG TGGGCCAGGC TGGAATTGCA 
TATTCCTGTA TCATTGTTAT TATGGCAACT GTTGTGACAA CCATCACAGG CTGCTCGACT 
TCTGCGATTG CAACTAATGG CTTTGTACGA GGAGGTGGAG CATATTACTT AATATCTCGA 
AGTCTGGGTC CAGAGTTTGG TGGCTCCATT GGTCTCATTT TTGCCTTTGC CAATGCTGTT 
GCTGTAGCCA TGTATGTTGT GGGTTTTGCC GAAACTGTTG TTGAGCTACT CATGGACTCT 
GGTCTATTAA TGATCGATCA AACAAATGAC ATCAGAGTCA TTGGGACCAT TACAGTCATT 
CTGCTTCTCG GCATTTCAGT GGCTGGAATG GAGTGGGAAG CCAAGGCCCA GATATTCCTA 
TTGGTCATAT TGATTACTGC CATTTTCAAC TACTTCATTG GGTCCTTCAT TGCTGTTGAC 
TCGAAGAAGA AATTTGGCTT CTTTAGTTAT GATGCTGGCA TCTTGGCGGA AAATTTTGGT 
CCAGACTTTC GAGGACAGAC GTTTTTCTCT GTTTTTTCCA TCTTCTTCCC AGCAGCCACA 
GGCATTTTGG CTGGTGCCAA CATCTCAGGA GATCTAGCAG ACCCTCAAAT GGCCATCCCA 
AAAGGAACGC TTTTGGCTAT TCTCATAACA GGCCTTGTGT ACGTGGGTGT GGCCATTTCT 
GCAGGTGCTT GCATCGTGCG TGATGCTACA GGAATCGAGA GTAACTTTAC TCTCATTTCA 
AACTGTACTG ATGCAGCCTG TAAGTATGGC TATGACTTCA GTTCCTGCAG ACCTACGGTT 
GAAGGAGAAG TATCTTCCTG CAAGTTTGGT CTTCACAATG ATTTCCAGGT TATGAGCGTG 
GTGTCAGGCT TCAGTCCTCT TATCAGTGCT GGGATTTTCT CTGCCACACT TTCCTCTGCC 
CTGGCATCTC TCGTCAGTGC CCCAAAAGTG TTTCAGGCTT TATGCAAAGA CAATATCTAT 
CCTGGAATTG CAATTTTTGG AAAGGGCTAT GGCAAGAACA ACGAGCCCCT GCGAGGATAT 
TTTCTTACCT TTGGCATTGC GTTAGCTTTC ATTCTAATTG CGGAGTTGAA CGTCATTGCC 
CCAATCATTT CCAACTTTTT CCTGGCATCA TACGCTCTCA TCAACTTCTC GGTGTTTCAC 
GCTTCGCTGG CCAATTCCCC TGGATGGAGG CCAAGCTTCA AGTACTACAA CATGTGGGCG 
TCTCTGGCCG GGGCAATCCT ATGTTGTGTT GTCATGTTTA TCATCAATTG GTGGGCAGCG 
CTTTTGACCA ACGTCATTGT CTTATCCCTT TACATCTACG TCAGCTACAA AAAACCAGAT 
GTGAATTGGG GTTCGTCAAC TCAAGCTCTG ACGTATCATC AGGCCTTAAC ACACAGTTTA 
CAGCTGTGCG GAGTAGCTGA CCACATTAAA ACCTTCAGGC CACAGTGTCT GGTGATGACT 
GGGGCTCCAA ACTCGCGTCC AGCTATTCTT CATCTGGTCC ATGCCTTCAC CAAAAATGTG 
GGGCTGATGT TATGTGGGCA TGTGCGCATT AGTTCTCGAC GGCCCAACTT CAAAGAGCTA 
AACAGCGATA TGTTGCGTTA CCAGAGGTGG CTCCTTAACA ACAACTCCAA GGCCTTTTAC 
ACATGTGTCG TTGCTGAAGA CCTCCGTCAA GGAACCCAGT ACATGCTGCA GGCTGCTGGA 
CTGGGTAGAT TGAGGCCCAA TACTCTGGTT ATTGGTTTCA AAAATGACTG GCGCACTGGA 
GACATAAAGG AGGTTGAGAC CTACATAAAC CTAATACATG ATGCGTTTGA TTTTCAGTAT 
GGTGTTGTCA TTCTCAGACT GCGAGAGGGA CTTGATATTT CTCACATTCA AGATGATTCT 
TCAGGTATGA AGGATGTGGT GGTTTCAGTG GACATAAGCA AAGACTCGGA TGGAGACTCA 
TCCAAACCCT CCTCCAAGGC CACCAGTGTT CAGAACAGCC CAGCGGTCCA GAAAGATGAG 
GATGATGATG GCAAAGCCCA CACTCAGCCG CTGTTGAAGA AAGATAAAAA GAGCCCGACA 
GTTCCTCTAA ATGTAGCTGA TCAGAGGTTG TTGGATGCCA GTCAACAGTT TCAGCAGAAA 
CAGGGGAAGG GGACAGTGGA TGTGTGGTGG CTCTTTGATG ACGGGGGTCT GACTCTACTG 
ATCCCCTATC TTATCGCCAA CAAGAAGAAA TGGAAGGACT GTAAGATCCG CGTGTTCATC 
GGTGGGAAGA TCAACAGAAT AGACCATGAC CGCAGAGCCA TGGCCACCTT GCTCAGCAAG 
TTCAGGATCG ATTTCTCTGA CATCACAGTC CTTGGTGACA TCAACACAAA GCCAAAGTCT 
GAGGGTTTGA CAGAGTTTGC TGAAATGATC GAGCCATATA AACTGAGAGA AGATGACATG 
GAGCAGGAAG CTGCTGAGAA GCTGAAAAGC GAGGAACCCT GGAGGATCAC AGACAATGAG 
CTGGAGCTCT ACAAAGCCAA GGGTAACCGT CAGATCAGAC TGAATGAGCT GTTGAAGGAG 
CACTCAAGCA CAGCCAACCT CATTGTCATG AGTATGCCGC TGGCCAGGAA AGGAGCCGTC 
TCCAGTGCCC TTTATATGGC CTGGCTAGAC ACACTATCCA AAGACCTGCC GCCCATCCTC 
TTAGTGCGCG GGAACCATCA GAGCGTCCTC ACGTTCTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334827.1
CDS	565..3969
Translation	MSASPPISAGDYLSAPEPDALKPAGPTPSQSRFQVDLVTESAGDGETTVGFDSSPPEYVAEPPPDGLRDSVSGGEEAKGRFRVVNFAASSPDAAPAETAQNGDTVMSEGSLHSSTGGQQHHHYDTHTNTYYLRTFGHNTIDAVPKIDFYRQTAAPLGEKLIRPTLSELHDELDKEPFEDGFANGEELTPAEESAAKDVSESKGVVKFGWIKGVLVRCMLNIWGVMLFIRMTWIVGQAGIAYSCIIVIMATVVTTITGCSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVELLMDSGLLMIDQTNDIRVIGTITVILLLGISVAGMEWEAKAQIFLLVILITAIFNYFIGSFIAVDSKKKFGFFSYDAGILAENFGPDFRGQTFFSVFSIFFPAATGILAGANISGDLADPQMAIPKGTLLAILITGLVYVGVAISAGACIVRDATGIESNFTLISNCTDAACKYGYDFSSCRPTVEGEVSSCKFGLHNDFQVMSVVSGFSPLISAGIFSATLSSALASLVSAPKVFQALCKDNIYPGIAIFGKGYGKNNEPLRGYFLTFGIALAFILIAELNVIAPIISNFFLASYALINFSVFHASLANSPGWRPSFKYYNMWASLAGAILCCVVMFIINWWAALLTNVIVLSLYIYVSYKKPDVNWGSSTQALTYHQALTHSLQLCGVADHIKTFRPQCLVMTGAPNSRPAILHLVHAFTKNVGLMLCGHVRISSRRPNFKELNSDMLRYQRWLLNNNSKAFYTCVVAEDLRQGTQYMLQAAGLGRLRPNTLVIGFKNDWRTGDIKEVETYINLIHDAFDFQYGVVILRLREGLDISHIQDDSSGMKDVVVSVDISKDSDGDSSKPSSKATSVQNSPAVQKDEDDDGKAHTQPLLKKDKKSPTVPLNVADQRLLDASQQFQQKQGKGTVDVWWLFDDGGLTLLIPYLIANKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDITVLGDINTKPKSEGLTEFAEMIEPYKLREDDMEQEAAEKLKSEEPWRITDNELELYKAKGNRQIRLNELLKEHSSTANLIVMSMPLARKGAVSSALYMAWLDTLSKDLPPILLVRGNHQSVLTFYS

Target ORF information:

RefSeq Version	XM_021479152.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (slc12a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479152.1

ORF Insert Sequence:

ATGTCGGCGT CACCTCCAAT CTCCGCGGGC GATTATCTCT CTGCGCCCGA ACCTGATGCG 
CTTAAACCCG CCGGTCCGAC TCCGAGCCAG AGCCGCTTCC AGGTGGATCT GGTGACAGAG 
TCCGCAGGGG ACGGTGAAAC CACTGTCGGC TTCGACTCAT CCCCACCCGA GTACGTTGCC 
GAGCCGCCGC CCGATGGGCT CCGGGATTCA GTATCTGGCG GCGAGGAAGC CAAGGGCCGG 
TTTCGGGTTG TGAACTTTGC GGCCTCAAGT CCGGACGCCG CGCCGGCGGA AACCGCGCAG 
AACGGGGACA CGGTGATGAG CGAGGGCAGT TTGCACTCCT CCACCGGCGG CCAGCAGCAC 
CATCACTACG ATACACACAC CAACACCTAC TACCTCCGTA CGTTCGGACA CAACACCATC 
GACGCCGTGC CCAAGATCGA CTTCTACCGG CAGACCGCCG CTCCGCTGGG CGAGAAACTC 
ATTAGACCCA CCCTCTCAGA ACTGCACGAC GAGCTGGACA AGGAGCCATT TGAGGATGGG 
TTTGCTAACG GCGAGGAGTT GACCCCTGCC GAGGAGTCTG CAGCTAAAGA TGTATCAGAG 
TCTAAAGGAG TGGTGAAGTT CGGATGGATC AAGGGCGTGC TGGTGCGCTG CATGTTAAAC 
ATATGGGGTG TGATGCTCTT CATTCGAATG ACATGGATCG TGGGCCAGGC TGGAATTGCA 
TATTCCTGTA TCATTGTTAT TATGGCAACT GTTGTGACAA CCATCACAGG CTGCTCGACT 
TCTGCGATTG CAACTAATGG CTTTGTACGA GGAGGTGGAG CATATTACTT AATATCTCGA 
AGTCTGGGTC CAGAGTTTGG TGGCTCCATT GGTCTCATTT TTGCCTTTGC CAATGCTGTT 
GCTGTAGCCA TGTATGTTGT GGGTTTTGCC GAAACTGTTG TTGAGCTACT CATGGACTCT 
GGTCTATTAA TGATCGATCA AACAAATGAC ATCAGAGTCA TTGGGACCAT TACAGTCATT 
CTGCTTCTCG GCATTTCAGT GGCTGGAATG GAGTGGGAAG CCAAGGCCCA GATATTCCTA 
TTGGTCATAT TGATTACTGC CATTTTCAAC TACTTCATTG GGTCCTTCAT TGCTGTTGAC 
TCGAAGAAGA AATTTGGCTT CTTTAGTTAT GATGCTGGCA TCTTGGCGGA AAATTTTGGT 
CCAGACTTTC GAGGACAGAC GTTTTTCTCT GTTTTTTCCA TCTTCTTCCC AGCAGCCACA 
GGCATTTTGG CTGGTGCCAA CATCTCAGGA GATCTAGCAG ACCCTCAAAT GGCCATCCCA 
AAAGGAACGC TTTTGGCTAT TCTCATAACA GGCCTTGTGT ACGTGGGTGT GGCCATTTCT 
GCAGGTGCTT GCATCGTGCG TGATGCTACA GGAATCGAGA GTAACTTTAC TCTCATTTCA 
AACTGTACTG ATGCAGCCTG TAAGTATGGC TATGACTTCA GTTCCTGCAG ACCTACGGTT 
GAAGGAGAAG TATCTTCCTG CAAGTTTGGT CTTCACAATG ATTTCCAGGT TATGAGCGTG 
GTGTCAGGCT TCAGTCCTCT TATCAGTGCT GGGATTTTCT CTGCCACACT TTCCTCTGCC 
CTGGCATCTC TCGTCAGTGC CCCAAAAGTG TTTCAGGCTT TATGCAAAGA CAATATCTAT 
CCTGGAATTG CAATTTTTGG AAAGGGCTAT GGCAAGAACA ACGAGCCCCT GCGAGGATAT 
TTTCTTACCT TTGGCATTGC GTTAGCTTTC ATTCTAATTG CGGAGTTGAA CGTCATTGCC 
CCAATCATTT CCAACTTTTT CCTGGCATCA TACGCTCTCA TCAACTTCTC GGTGTTTCAC 
GCTTCGCTGG CCAATTCCCC TGGATGGAGG CCAAGCTTCA AGTACTACAA CATGTGGGCG 
TCTCTGGCCG GGGCAATCCT ATGTTGTGTT GTCATGTTTA TCATCAATTG GTGGGCAGCG 
CTTTTGACCA ACGTCATTGT CTTATCCCTT TACATCTACG TCAGCTACAA AAAACCAGAT 
GTGAATTGGG GTTCGTCAAC TCAAGCTCTG ACGTATCATC AGGCCTTAAC ACACAGTTTA 
CAGCTGTGCG GAGTAGCTGA CCACATTAAA ACCTTCAGGC CACAGTGTCT GGTGATGACT 
GGGGCTCCAA ACTCGCGTCC AGCTATTCTT CATCTGGTCC ATGCCTTCAC CAAAAATGTG 
GGGCTGATGT TATGTGGGCA TGTGCGCATT AGTTCTCGAC GGCCCAACTT CAAAGAGCTA 
AACAGCGATA TGTTGCGTTA CCAGAGGTGG CTCCTTAACA ACAACTCCAA GGCCTTTTAC 
ACATGTGTCG TTGCTGAAGA CCTCCGTCAA GGAACCCAGT ACATGCTGCA GGCTGCTGGA 
CTGGGTAGAT TGAGGCCCAA TACTCTGGTT ATTGGTTTCA AAAATGACTG GCGCACTGGA 
GACATAAAGG AGGTTGAGAC CTACATAAAC CTAATACATG ATGCGTTTGA TTTTCAGTAT 
GGTGTTGTCA TTCTCAGACT GCGAGAGGGA CTTGATATTT CTCACATTCA AGATGATTCT 
TCAGGTATGA AGGATGTGGT GGTTTCAGTG GACATAAGCA AAGACTCGGA TGGAGACTCA 
TCCAAACCCT CCTCCAAGGC CACCAGTGTT CAGAACAGCC CAGCGGTCCA GAAAGATGAG 
GATGATGATG GCAAAGCCCA CACTCAGCCG CTGTTGAAGA AAGATAAAAA GAGCCCGACA 
GTTCCTCTAA ATGTAGCTGA TCAGAGGTTG TTGGATGCCA GTCAACAGTT TCAGCAGAAA 
CAGGGGAAGG GGACAGTGGA TGTGTGGTGG CTCTTTGATG ACGGGGGTCT GACTCTACTG 
ATCCCCTATC TTATCGCCAA CAAGAAGAAA TGGAAGGACT GTAAGATCCG CGTGTTCATC 
GGTGGGAAGA TCAACAGAAT AGACCATGAC CGCAGAGCCA TGGCCACCTT GCTCAGCAAG 
TTCAGGATCG ATTTCTCTGA CATCACAGTC CTTGGTGACA TCAACACAAA GCCAAAGTCT 
GAGGGTTTGA CAGAGTTTGC TGAAATGATC GAGCCATATA AACTGAGAGA AGATGACATG 
GAGCAGGAAG CTGCTGAGAA GCTGAAAAGC GAGGAACCCT GGAGGATCAC AGACAATGAG 
CTGGAGCTCT ACAAAGCCAA GGGTAACCGT CAGATCAGAC TGAATGAGCT GTTGAAGGAG 
CACTCAAGCA CAGCCAACCT CATTGTCATG AGTATGCCGC TGGCCAGGAA AGGAGCCGTC 
TCCAGTGCCC TTTATATGGC CTGGCTAGAC ACACTATCCA AAGACCTGCC GCCCATCCTC 
TTAGTGCGCG GGAACCATCA GAGCGTCCTC ACGTTCTACT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycine is able to induce both a motility speed in- and decrease during zebrafish neuronal migration.
Communicative & integrative biology11(3)1-7(2018)
Theisen U,Hey S,Hennig CD,Schnabel R,Köster RW

In Vivo Characterization of an AHR-Dependent Long Noncoding RNA Required for Proper
Molecular pharmacology91(6)609-619(2017 06)
Garcia GR,Goodale BC,Wiley MW,La Du JK,Hendrix DA,Tanguay RL

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Characterization of the γ-aminobutyric acid signaling system in the zebrafish (Danio rerio Hamilton) central nervous system by reverse transcription-quantitative polymerase chain reaction.
Neuroscience343300-321(2017 02)
Cocco A,Rönnberg AM,Jin Z,André GI,Vossen LE,Bhandage AK,Thörnqvist PO,Birnir B,Winberg S

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
PloS one12(6)e0179517(2017)
Sullivan C,Lage CR,Yoder JA,Postlethwait JH,Kim CH

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