msh2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	msh2
Entrez Gene ID	406845
Full Name	mutS homolog 2 (E. coli)
Synonyms	wu:fc06b02,wu:fc13e09,zgc:55333
General protein information	Preferred Names mutS homolog 2 (E. coli) Names DNA mismatch repair protein Msh2 mismatch repair protein Msh2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213524.1	NP_998689.1	DNA mismatch repair protein Msh2

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F02706g	XP_455202.1
Schizosaccharomyces pombe (fission yeast)	msh2	XP_001713136.1
Eremothecium gossypii	AGOS_AFR603C	NP_986150.1
Magnaporthe oryzae (rice blast fungus)	MGG_00879	XP_003718090.1
Rattus norvegicus (Norway rat)	Msh2	NP_112320.1
Gallus gallus (chicken)	MSH2	XP_426110.4
Saccharomyces cerevisiae (baker's yeast)	MSH2	NP_014551.1
Danio rerio (zebrafish)	msh2	NP_998689.1
Arabidopsis thaliana (thale cress)	MSH2	NP_566804.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010282	XP_319476.4
Homo sapiens (human)	MSH2	NP_000242.1
Canis lupus familiaris (dog)	MSH2	XP_538482.2
Bos taurus (cattle)	MSH2	NP_001029756.1
Mus musculus (house mouse)	Msh2	NP_032654.1
Drosophila melanogaster (fruit fly)	spel1	NP_001246031.1
Neurospora crassa	NCU02230	XP_959643.1
Xenopus tropicalis (tropical clawed frog)	msh2	XP_002935427.2

Gene Ontology
Bibliography

Related articles in PubMed

Cellular responses to ionizing radiation change quickly over time during early development in zebrafish.
Honjo Y, Ichinohe T
Cell biology international43(5)516-527(2019 May)

Heat stress upregulates G-T mismatch binding activities in zebrafish (Danio rerio) embryos preexposed and nonexposed to a sublethal level of cadmium (Cd).
Ho TN, Paul GV, Chen YH, Hsu T
Chemosphere218179-188(2019 Mar)

Ribosomal Proteins Rpl22 and Rpl22l1 Control Morphogenesis by Regulating Pre-mRNA Splicing.
Zhang Y, O'Leary MN, Peri S, Wang M, Zha J, Melov S, Kappes DJ, Feng Q, Rhodes J, Amieux PS, Morris DR, Kennedy BK, Wiest DL
Cell reports18(2)545-556(2017 01)

Epigenetic Studies Point to DNA Replication/Repair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes.
Leontovich AA, Intine RV, Sarras MP Jr
Journal of diabetes research20162860780(2016)

Demonstration of the Coexistence of Duplicated LH Receptors in Teleosts, and Their Origin in Ancestral Actinopterygians.
Maugars G, Dufour S
PloS one10(8)e0135184(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following msh2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the msh2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00528	NM_213524.1 Latest version!	Danio rerio mutS homolog 2 (E. coli) (msh2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

msh2 ( NM_213524.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998689.1 Danio rerio mutS homolog 2 (E. coli) (msh2), mRNA.

Price & Availability↑

CloneID	ODa00528
Clone ID Related Accession (Same CDS sequence)	NM_213524.1
Accession Version	NM_213524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1578067200000
Organism	Danio rerio(zebrafish)
Product	DNA mismatch repair protein Msh2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC044370.1. On Nov 22, 2004 this sequence version replaced NM_214778.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC044370.1, AF412833.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGTGC AACCTAAACA GAATCTGTCT ATGGACAGCG CGTCTGAACA TGGATTCCTC 
AATTTTTACT TTTCGATGTC CGACAAACCA GACACCACGG TGCGAGTGTT TGATAGAAAC 
GATTACTATA CTGTGCACGG GAAAGATGCG ATTTTTGCAG CTAAAGAGGT TTTCAAGACT 
AATGGAGTCA TCAAAAATCT GGGTTCAGGG AACAGGAGGT TGGAAAGTGT GGTCCTCAGT 
AAGATGAACT TTGAGTCATT TGTGAGGGAT TTGCTGCTGG TTAGACAATA CCGGGTGGAG 
GTTTACAAGA ACGCCAGTAA GAGCAGCAAA GAGCATGACT GGCAAATCGC TTTCAAGGCA 
TCTCCAGGAA ACCTTACTCA GTTTGAGGAG ATTCTGTTTG GCAGTGGCGG AGGGCCTGCT 
GAGGGTGCTG TCGGTGTGGT GGGTGTTCGT CTTGGCACTG GCACTGATGG CCAGCGTGTT 
GTCGGTGTTG GTTATGTGGA CAGTACCCTC AGGAAACTTG GTGTGTGCGA GTTCCCTGAT 
AACGATCAGT TCTCCAACCT GGAGGCTCTA CTTGTGCAAA TCGGCCCCAA AGAGTGTGTG 
CTTCCTGCAG GAGATTCAGG AGGAGACCAG GGCAAACTTA AACAGGTGGT GCAGCGTGGT 
GGCATTCTCC TCACAGACAG GAAGAAGTCA GAGTTCACAA CTAAAGACAT TGTGCAGGAT 
CTGAATCGGC TACTGAAGGC CCGTAAAGGA GAGACTGTGT CCAGCGCTGC CCTGCCTGAG 
ATGGAGAAAA AGATTGCGAT GTCTTGCTTA GAAGCAGTCA TCAAATACCT GGAGCTTCTC 
GCAGATGAGG CCAATTTTGG GTCTTTCAAG ATGACCACCT TTGACCTCAA CCAGTACATG 
CGCCTGGATA ATGCTGCTGT ACAGGCTCTC AATCTCTTTC AGGGTTCCTC TGATGATGCT 
ACAGGCACTC ACTCTCTGGC TGGACTTCTG AACAAATGTC GGACTCCACA AGGACAGCGA 
CTGGTCAACC AATGGATCAA ACAGCCTCTC ATTGACAAAA ACAAGATAGA AGAAAGACTG 
GACCTTGTGG AGACATTTGT GGAGGACTCG GAGCTAAGGA AAAGTTGTCA AGAGGATCTA 
CTTCGTCGCT TTCCTGACTT AAATCGGATG GCAAAGAAGT TCCAGCGTCA GAGCTCCAAT 
TTACAGGACT GTTACCGGGT CTATCAATCT GTGGGGCAAC TGCCCAATGT GGTGCTGGCA 
CTGGAGAGAT ACAGTGGAAA ACATCAGGTC CTCCTGCATG CAGCATTCAT CTCTCCACTA 
AACGACCTCA TCTCAGACTT CTCCAAATTT CAGGAGATGA TTGAAACCAC ACTTGACATG 
AACCAGGTTG AGCATCACGA GTTTCTGGTG AAACCTTCCT TTGATCCAAC TTTGAGTGAT 
CTCAGGGAAA ACATGGATCG TTTGGAGAAA GCCATGCAGG CTGCCTTGAG CAGCGCAGCT 
CGGGAGCTTG GGTTGGAAGC AGCCAAGACT GTAAAGCTTG AATCCAATGC ACAAATTGGT 
TATTTTTTCC GAGTTACGTG CAAAGAAGAA AAGAGTCTTC GCAACAACAA GAAATTCACT 
ACTTTAGACG TTCAGAAGAA TGGAGTACGT TTCACTAATA GTAAGCTTAG CTCGTTGAAT 
GAAGAATACA CAAAAAGCAG AGAAGAGTAT GAAGAGGCTC AGAATGCCAT CGTCAAGGAA 
ATCATCAGCA TTGCTGCAGG TTATGTGGAT CCAGTACAGA CTCTGAATGA GGTGATCGCT 
CAGCTGGATG CAGTGTTAAG CTTCGCTGTG GTGTCACATG CTGCCCCAGT GCCTTTCATA 
AGACCAAAAA TACTAGAGAA GGGTTCAGGG AGACTCGTTC TGAAGGCAGC AAGACATCCC 
TGTGTTGAAG CCCAGGATGA AGTGGCTTTC ATTCCCAATG ATGTCACCTT CATAAGAGGA 
GAGAAGATGT TTCATATCAT CACAGGTCCA AGTATGGGTG GTAAATCTAC CTACATCCGT 
CAAGTGGGTG TGATTGTGCT GATGGCACAG ATTGGCTGTT TTGTGCCATG TGATGAGGCA 
GAGCTGAGCG TGGTGGACTG TGTTCTTGCT CGAGTAGGTG CGGGTGACAG CCAGATTAAA 
GGAGTGTCCA CTTTTATGGC TGAGATGCTG GAGACAGCAG CTATTCTTCG GTCAGCCACT 
GAGGATTCTC TCATCACTAT TGATGAATTG GGCAGAGGCA CATCCACATA TGATGGCTTT 
GGTCTGGCCT GGGCGATCTC AGAGTATATC GCCACCCGCC TCAAGTCCTT CTGCCTGTTC 
GCAACTCACT TCCATGAGCT GACGGCGCTG GCACAGCAGG TGCCCACTGT CCGCAACCTC 
CATGTCACCG CGCTGACCAC AGACAGCACT CTTACCATGC TCTACAAGGT CAAAAAGGGT 
GTGTGTGACC AGAGCTTTGG GATTCATGTG GCGGAGCTTG CTAGCTTCCC CAAACATGTA 
ATTGCAAATG CGCGGGAGAA GGCTTTGGAG CTTGAAGAAT TTCAAGACAT CTCCAGTGTT 
GGGGAGGAAG CAGGGCCCAA AGCCAAGAAA CGCTGCATGG AGAAACAGGA GGGAGAGAGG 
ATCATTGAGG CTTTTCTGGC TAAGGTTAAA TCAATGCCTG TTGATGGGAT GTCTGACAAG 
GCTGTCAAAG AGGAGCTGCG CAAACTCAAG GCAGAGGTCA TCAGCCAAAA CAATAGCTTC 
GTCAATGAGA TAGTGTCACG CTCTGGCAAG GTCAAACTCA GTTCAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998689.1
CDS	41..2851
Translation	MAVQPKQNLSMDSASEHGFLNFYFSMSDKPDTTVRVFDRNDYYTVHGKDAIFAAKEVFKTNGVIKNLGSGNRRLESVVLSKMNFESFVRDLLLVRQYRVEVYKNASKSSKEHDWQIAFKASPGNLTQFEEILFGSGGGPAEGAVGVVGVRLGTGTDGQRVVGVGYVDSTLRKLGVCEFPDNDQFSNLEALLVQIGPKECVLPAGDSGGDQGKLKQVVQRGGILLTDRKKSEFTTKDIVQDLNRLLKARKGETVSSAALPEMEKKIAMSCLEAVIKYLELLADEANFGSFKMTTFDLNQYMRLDNAAVQALNLFQGSSDDATGTHSLAGLLNKCRTPQGQRLVNQWIKQPLIDKNKIEERLDLVETFVEDSELRKSCQEDLLRRFPDLNRMAKKFQRQSSNLQDCYRVYQSVGQLPNVVLALERYSGKHQVLLHAAFISPLNDLISDFSKFQEMIETTLDMNQVEHHEFLVKPSFDPTLSDLRENMDRLEKAMQAALSSAARELGLEAAKTVKLESNAQIGYFFRVTCKEEKSLRNNKKFTTLDVQKNGVRFTNSKLSSLNEEYTKSREEYEEAQNAIVKEIISIAAGYVDPVQTLNEVIAQLDAVLSFAVVSHAAPVPFIRPKILEKGSGRLVLKAARHPCVEAQDEVAFIPNDVTFIRGEKMFHIITGPSMGGKSTYIRQVGVIVLMAQIGCFVPCDEAELSVVDCVLARVGAGDSQIKGVSTFMAEMLETAAILRSATEDSLITIDELGRGTSTYDGFGLAWAISEYIATRLKSFCLFATHFHELTALAQQVPTVRNLHVTALTTDSTLTMLYKVKKGVCDQSFGIHVAELASFPKHVIANAREKALELEEFQDISSVGEEAGPKAKKRCMEKQEGERIIEAFLAKVKSMPVDGMSDKAVKEELRKLKAEVISQNNSFVNEIVSRSGKVKLSSA

Target ORF information:

RefSeq Version	NM_213524.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mutS homolog 2 (E. coli) (msh2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213524.1

ORF Insert Sequence:

ATGGCGGTGC AACCTAAACA GAATCTGTCT ATGGACAGCG CGTCTGAACA TGGATTCCTC 
AATTTTTACT TTTCGATGTC CGACAAACCA GACACCACGG TGCGAGTGTT TGATAGAAAC 
GATTACTATA CTGTGCACGG GAAAGATGCG ATTTTTGCAG CTAAAGAGGT TTTCAAGACT 
AATGGAGTCA TCAAAAATCT GGGTTCAGGG AACAGGAGGT TGGAAAGTGT GGTCCTCAGT 
AAGATGAACT TTGAGTCATT TGTGAGGGAT TTGCTGCTGG TTAGACAATA CCGGGTGGAG 
GTTTACAAGA ACGCCAGTAA GAGCAGCAAA GAGCATGACT GGCAAATCGC TTTCAAGGCA 
TCTCCAGGAA ACCTTACTCA GTTTGAGGAG ATTCTGTTTG GCAGTGGCGG AGGGCCTGCT 
GAGGGTGCTG TCGGTGTGGT GGGTGTTCGT CTTGGCACTG GCACTGATGG CCAGCGTGTT 
GTCGGTGTTG GTTATGTGGA CAGTACCCTC AGGAAACTTG GTGTGTGCGA GTTCCCTGAT 
AACGATCAGT TCTCCAACCT GGAGGCTCTA CTTGTGCAAA TCGGCCCCAA AGAGTGTGTG 
CTTCCTGCAG GAGATTCAGG AGGAGACCAG GGCAAACTTA AACAGGTGGT GCAGCGTGGT 
GGCATTCTCC TCACAGACAG GAAGAAGTCA GAGTTCACAA CTAAAGACAT TGTGCAGGAT 
CTGAATCGGC TACTGAAGGC CCGTAAAGGA GAGACTGTGT CCAGCGCTGC CCTGCCTGAG 
ATGGAGAAAA AGATTGCGAT GTCTTGCTTA GAAGCAGTCA TCAAATACCT GGAGCTTCTC 
GCAGATGAGG CCAATTTTGG GTCTTTCAAG ATGACCACCT TTGACCTCAA CCAGTACATG 
CGCCTGGATA ATGCTGCTGT ACAGGCTCTC AATCTCTTTC AGGGTTCCTC TGATGATGCT 
ACAGGCACTC ACTCTCTGGC TGGACTTCTG AACAAATGTC GGACTCCACA AGGACAGCGA 
CTGGTCAACC AATGGATCAA ACAGCCTCTC ATTGACAAAA ACAAGATAGA AGAAAGACTG 
GACCTTGTGG AGACATTTGT GGAGGACTCG GAGCTAAGGA AAAGTTGTCA AGAGGATCTA 
CTTCGTCGCT TTCCTGACTT AAATCGGATG GCAAAGAAGT TCCAGCGTCA GAGCTCCAAT 
TTACAGGACT GTTACCGGGT CTATCAATCT GTGGGGCAAC TGCCCAATGT GGTGCTGGCA 
CTGGAGAGAT ACAGTGGAAA ACATCAGGTC CTCCTGCATG CAGCATTCAT CTCTCCACTA 
AACGACCTCA TCTCAGACTT CTCCAAATTT CAGGAGATGA TTGAAACCAC ACTTGACATG 
AACCAGGTTG AGCATCACGA GTTTCTGGTG AAACCTTCCT TTGATCCAAC TTTGAGTGAT 
CTCAGGGAAA ACATGGATCG TTTGGAGAAA GCCATGCAGG CTGCCTTGAG CAGCGCAGCT 
CGGGAGCTTG GGTTGGAAGC AGCCAAGACT GTAAAGCTTG AATCCAATGC ACAAATTGGT 
TATTTTTTCC GAGTTACGTG CAAAGAAGAA AAGAGTCTTC GCAACAACAA GAAATTCACT 
ACTTTAGACG TTCAGAAGAA TGGAGTACGT TTCACTAATA GTAAGCTTAG CTCGTTGAAT 
GAAGAATACA CAAAAAGCAG AGAAGAGTAT GAAGAGGCTC AGAATGCCAT CGTCAAGGAA 
ATCATCAGCA TTGCTGCAGG TTATGTGGAT CCAGTACAGA CTCTGAATGA GGTGATCGCT 
CAGCTGGATG CAGTGTTAAG CTTCGCTGTG GTGTCACATG CTGCCCCAGT GCCTTTCATA 
AGACCAAAAA TACTAGAGAA GGGTTCAGGG AGACTCGTTC TGAAGGCAGC AAGACATCCC 
TGTGTTGAAG CCCAGGATGA AGTGGCTTTC ATTCCCAATG ATGTCACCTT CATAAGAGGA 
GAGAAGATGT TTCATATCAT CACAGGTCCA AGTATGGGTG GTAAATCTAC CTACATCCGT 
CAAGTGGGTG TGATTGTGCT GATGGCACAG ATTGGCTGTT TTGTGCCATG TGATGAGGCA 
GAGCTGAGCG TGGTGGACTG TGTTCTTGCT CGAGTAGGTG CGGGTGACAG CCAGATTAAA 
GGAGTGTCCA CTTTTATGGC TGAGATGCTG GAGACAGCAG CTATTCTTCG GTCAGCCACT 
GAGGATTCTC TCATCACTAT TGATGAATTG GGCAGAGGCA CATCCACATA TGATGGCTTT 
GGTCTGGCCT GGGCGATCTC AGAGTATATC GCCACCCGCC TCAAGTCCTT CTGCCTGTTC 
GCAACTCACT TCCATGAGCT GACGGCGCTG GCACAGCAGG TGCCCACTGT CCGCAACCTC 
CATGTCACCG CGCTGACCAC AGACAGCACT CTTACCATGC TCTACAAGGT CAAAAAGGGT 
GTGTGTGACC AGAGCTTTGG GATTCATGTG GCGGAGCTTG CTAGCTTCCC CAAACATGTA 
ATTGCAAATG CGCGGGAGAA GGCTTTGGAG CTTGAAGAAT TTCAAGACAT CTCCAGTGTT 
GGGGAGGAAG CAGGGCCCAA AGCCAAGAAA CGCTGCATGG AGAAACAGGA GGGAGAGAGG 
ATCATTGAGG CTTTTCTGGC TAAGGTTAAA TCAATGCCTG TTGATGGGAT GTCTGACAAG 
GCTGTCAAAG AGGAGCTGCG CAAACTCAAG GCAGAGGTCA TCAGCCAAAA CAATAGCTTC 
GTCAATGAGA TAGTGTCACG CTCTGGCAAG GTCAAACTCA GTTCAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cellular responses to ionizing radiation change quickly over time during early development in zebrafish.
Cell biology international43(5)516-527(2019 May)
Honjo Y,Ichinohe T

Heat stress upregulates G-T mismatch binding activities in zebrafish (Danio rerio) embryos preexposed and nonexposed to a sublethal level of cadmium (Cd).
Chemosphere218179-188(2019 Mar)
Ho TN,Paul GV,Chen YH,Hsu T

Ribosomal Proteins Rpl22 and Rpl22l1 Control Morphogenesis by Regulating Pre-mRNA Splicing.
Cell reports18(2)545-556(2017 01)
Zhang Y,O'Leary MN,Peri S,Wang M,Zha J,Melov S,Kappes DJ,Feng Q,Rhodes J,Amieux PS,Morris DR,Kennedy BK,Wiest DL

Epigenetic Studies Point to DNA Replication/Repair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes.
Journal of diabetes research20162860780(2016)
Leontovich AA,Intine RV,Sarras MP Jr

Demonstration of the Coexistence of Duplicated LH Receptors in Teleosts, and Their Origin in Ancestral Actinopterygians.
PloS one10(8)e0135184(2015)
Maugars G,Dufour S

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msh2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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