hk1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hk1
Entrez Gene ID	406791
Full Name	hexokinase 1
Synonyms	im:7148527,wu:fc09d08,wu:fc16e02,wu:fc21e02,wu:fq14b11,zgc:55790,zgc:77618
General protein information	Preferred Names hexokinase 1 Names hexokinase-1 DrHXK1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213252.1	NP_998417.1	hexokinase-1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os05g0522500	NP_001056082.1
Drosophila melanogaster (fruit fly)	Hex-A	NP_524848.1
Saccharomyces cerevisiae (baker's yeast)	HXK2	NP_011261.1
Xenopus tropicalis (tropical clawed frog)	hk1	NP_001096201.1
Magnaporthe oryzae (rice blast fungus)	MGG_09289	XP_003709938.1
Homo sapiens (human)	HK1	NP_000179.2
Bos taurus (cattle)	HK1	NP_001012686.1
Eremothecium gossypii	AGOS_AFR279C	NP_985826.1
Macaca mulatta (Rhesus monkey)	HK1	XP_001110396.2
Mus musculus (house mouse)	Hk1	NP_001139572.1
Gallus gallus (chicken)	HK1	NP_989432.1
Kluyveromyces lactis	KLLA0D11352g	XP_453567.1
Oryza sativa (rice)	Os01g0742500	NP_001044214.1
Canis lupus familiaris (dog)	HK1	XP_536376.3
Rattus norvegicus (Norway rat)	Hk1	NP_036866.1
Arabidopsis thaliana (thale cress)	HXK2	NP_179576.1
Schizosaccharomyces pombe (fission yeast)	hxk1	NP_592948.1
Neurospora crassa	NCU02542	XP_965673.2
Pan troglodytes (chimpanzee)	HK1	XP_001169264.2
Danio rerio (zebrafish)	hk1	NP_998417.1
Drosophila melanogaster (fruit fly)	Hex-t2	NP_733151.2
Drosophila melanogaster (fruit fly)	Hex-C	NP_524674.1
Saccharomyces cerevisiae (baker's yeast)	HXK1	NP_116711.3
Arabidopsis thaliana (thale cress)	HXK1	NP_194642.1

Gene Ontology
Bibliography

Related articles in PubMed

The effects of Roundup® in embryo development and energy metabolism of the zebrafish (Danio rerio).
Panetto OS, Gomes HF, Fraga Gomes DS, Campos E, Romeiro NC, Costa EP, do Carmo PRL, Feitosa NM, Moraes J
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP22274-81(2019 Aug)

Toxic effects of boscalid in adult zebrafish (Danio rerio) on carbohydrate and lipid metabolism.
Qian L, Zhang J, Chen X, Qi S, Wu P, Wang C, Wang C
Environmental pollution (Barking, Essex : 1987)247775-782(2019 Apr)

Dietary administration of Bacillus amyloliquefaciens R8 reduces hepatic oxidative stress and enhances nutrient metabolism and immunity against Aeromonas hydrophila and Streptococcus agalactiae in zebrafish (Danio rerio).
Lin YS, Saputra F, Chen YC, Hu SY
Fish & shellfish immunology86410-419(2019 Mar)

Effects of polystyrene microplastics on the composition of the microbiome and metabolism in larval zebrafish.
Wan Z, Wang C, Zhou J, Shen M, Wang X, Fu Z, Jin Y
Chemosphere217646-658(2019 Feb)

Impairment of bisphenol F on the glucose metabolism of zebrafish larvae.
Zhao F, Wang H, Wei P, Jiang G, Wang W, Zhang X, Ru S
Ecotoxicology and environmental safety165386-392(2018 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01430	NM_213252.1 Latest version!	Danio rerio hexokinase 1 (hk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hk1 ( NM_213252.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998417.1 Danio rerio hexokinase 1 (hk1), mRNA.

Price & Availability↑

CloneID	ODa01430
Clone ID Related Accession (Same CDS sequence)	NM_213252.1
Accession Version	NM_213252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1578067200000
Organism	Danio rerio(zebrafish)
Product	hexokinase-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC048065.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC048065.1, BC067330.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGATAGCGG CACAGCTTCT GGCCTACTAC TTCACTGAAC TGAAGGATGA CCAGGTCAAA 
AAGATTGACA AATACTTGTA CGCCATGCGG TTCTCTGACG AGACGCTGCG CGATATTATG 
GCTCGTTTCC GACGGGAAAT GGAGAACGGA TTGGCACGAG ACACCAACCC GACAGCCACC 
GCTAAAATGC TGCCCACCTT TGTAAGGTCC ATTCCTGATG GATCAGAGAA AGGAGATTTC 
ATCGCACTGG ATCTGGGTGG CTCTAATTTC CGTATTCTGC GTGTAAAAGT GTCTCATGAG 
AAGAAACAGA CAGTGCAGAT GGAAAGTCAG ATTTACGAGA CTCCAGAAGA CATCATCCAC 
GGCAGCAGAT CACGGTTGTT TGACCATGTT GCAGAATGTC TTGGAGACTT CATGGAGAAA 
CAAAAAATTA AAGACAAGAA GCTTCCTGTG GGCTTCACCT TCTCATTTCC TTGTTCACAA 
AGCAAACTGG ATGAGGCTGT TTTGCTGACA TGGACGAAAC GCTTCAAGGT TAATGGAGTA 
GAGGGAATGG ATGTAGTGAA GCTTCTCAAT AAGGCCATTA AAAAACGTGG AGATTACGAA 
GCAGATATAA TGGCTGTTGT CAATGACACT GTTGGAACTA TGATGACCTG CGGATTTGAT 
GACCAGCGAT GTGAAGTCGG CATTATCATA GGTACCGGCA CTAATGCATG CTACATGGAG 
GAGCTCAGAC ACATGGACAT GGTGGAGGGA GATGAAGGCA GGATGTGTAT TAATACTGAA 
TGGGGGGCGT TTGGAGATGA CGGCACATTG GAGGACATCC GGACTGAGTT TGACAGGGAG 
ATTGACCGAG GATCCCTCAA CCCTGGCAAG CAACTGTTTG AGAAGATGGT GAGTGGGATG 
TACATGGGTG AGCTGGTGAG GCTGATCCTG GTCAAAATGG CTAAAGAAGG CCTGCTGTTT 
GAAGGCCGCA TCACACCAGA GCTGCTTACA AAAGGAAAAA TCGAAACCAA GCACGTTTCT 
GCAATTGAGA AGAGCAAGGA AGGACTAACC AAAGCCAAGG AGATTTTGAC ACGCTTGGGT 
GTAGAGCCGT CTGAGGACGA CTGCATTGCT GTGCAGCATG TTTGTGCCAT TGTCTCTTTT 
CGCTCTGCTA ATCTAATTGC TGCCACTTTG GGTGCAATCC TGACACGTCT GAAGGACAAC 
AAGAACACCC CACGTCTCCG CACCACCGTG GGCATTGATG GCTCCCTATA CAAGATGCAC 
CCACAGTATG CCCGTCGGTT GCACAAAACA GTGCGTCGTC TGGTGCCAGA GTCTGATGTT 
CGCTTCTTGC TGTCTGAGAG TGGAAGTGGA AAGGGAGCTG CACTGGTGAC AGCCTGGGCT 
TACCGTTTGG CGGACCAGGA GCGTCAAATT GCAGAAACCC TGGAAGAGTT CAGGTTGACC 
AAAGACCAGC TGCTAGAGGT GAAAAAGAGG ATGAGAACTG AGATTCAGAA TGGACTGAGC 
AAGAGCACCC AAAACACAGC CACCGTCAAA ATGCTGCCCA CATATGTGCG GAGTACCCCT 
GATGGATCAG AAAACGGTGA CTTTTTGGCT CTGGATCTTG GAGGTACAAA CTTCAGGGTC 
CTTTTGGTAA AGATTCGCAG TGGCAAGAGA CGAACTGTGG AGATGCATAA TAAAATTTAT 
GCCATTCCCA TAGAAGTAAT GCAAGGAACC GGAGAGGAGC TTTTTGATCA CATCGTGTAC 
TGCATATCTG ACTTCCTGGA CTACATGGGA ATGAAGAATG CCCGTCTGCC TCTGGGCTTT 
ACCTTTTCTT TCCCCTGCAG ACAAACTAGT CTTGATGCGG GACTCTTGGT GAATTGGACG 
AAGGGCTTTA AAGCCACAGA CTGTGAAGGT GAAGATGTTG TCGGCCTTCT GAGAGAGGGG 
ATCAAGAGGA GAGAGGAGTT TGATTTGGAT GTTGTAGCCA TAGTAAATGA CACAGTGGGC 
ACAATGATGA CCTGCGCATA TGAAGAGCCC ACGTGTGAGG TTGGGCTAAT CGCAGGAACG 
GGCAGCAATG CCTGCTACAT GGAGGAAATG CGAAACATCG AGACAGTAAG TGGAGAGGAG 
GGCAGGATGT GTGTGAATAT GGAGTGGGGA GCATTTGGAG ATAATGGCTG TCTGGATGAC 
ATCCGGACGA AGTATGACGA TGCTGTGGAT GACCTCTCCC TCAATGCCGG CAAACAAAAG 
TATGAGAAGA TGTGCAGTGG CATGTACCTG GGTGAGATTG TTCGAAACAT CTTGATCGAC 
TTGACGAAGC GAGGCTTCCT CTTCAGGGGA CAGATCTCTG AAACACTGAA GACCAGAGGC 
ATCTTCGAAA CCAAGTTCCT GTCCCAAATT GAGAGTGACC GTTTGGCGCT ACTGCAGGTC 
AGGTCTATTC TCCAGCATCT GGGTCTGGAC AGCACATGCG ATGACAGTAT CATCGTCAAG 
GAGGTTTGTG GAGCTGTTTC TCGTCGAGCA GCTCAGCTCT GTGGAGCAGG AATGGCTGCT 
GTTGTGGATA AAATCCGGGA GAACCGTGGA CTGGATCATC TGGACATCAC TGTTGGAGTG 
GATGGCACCC TGTATAAGCT CCATCCACAT TTCTCTAGGA TAATGCATCA GACGGTGAAG 
GAGCTCGCCC CCAAATGCAA CGTCACTTTC CTTCTCTCAG AAGACGGAAG CGGTAAAGGT 
GCTGCCTTGA TCACCGCCGT TGGGTGTCGC CTCCGCCAGC AAGAACAGAA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998417.1
CDS	205..2961
Translation	MIAAQLLAYYFTELKDDQVKKIDKYLYAMRFSDETLRDIMARFRREMENGLARDTNPTATAKMLPTFVRSIPDGSEKGDFIALDLGGSNFRILRVKVSHEKKQTVQMESQIYETPEDIIHGSRSRLFDHVAECLGDFMEKQKIKDKKLPVGFTFSFPCSQSKLDEAVLLTWTKRFKVNGVEGMDVVKLLNKAIKKRGDYEADIMAVVNDTVGTMMTCGFDDQRCEVGIIIGTGTNACYMEELRHMDMVEGDEGRMCINTEWGAFGDDGTLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYMGELVRLILVKMAKEGLLFEGRITPELLTKGKIETKHVSAIEKSKEGLTKAKEILTRLGVEPSEDDCIAVQHVCAIVSFRSANLIAATLGAILTRLKDNKNTPRLRTTVGIDGSLYKMHPQYARRLHKTVRRLVPESDVRFLLSESGSGKGAALVTAWAYRLADQERQIAETLEEFRLTKDQLLEVKKRMRTEIQNGLSKSTQNTATVKMLPTYVRSTPDGSENGDFLALDLGGTNFRVLLVKIRSGKRRTVEMHNKIYAIPIEVMQGTGEELFDHIVYCISDFLDYMGMKNARLPLGFTFSFPCRQTSLDAGLLVNWTKGFKATDCEGEDVVGLLREGIKRREEFDLDVVAIVNDTVGTMMTCAYEEPTCEVGLIAGTGSNACYMEEMRNIETVSGEEGRMCVNMEWGAFGDNGCLDDIRTKYDDAVDDLSLNAGKQKYEKMCSGMYLGEIVRNILIDLTKRGFLFRGQISETLKTRGIFETKFLSQIESDRLALLQVRSILQHLGLDSTCDDSIIVKEVCGAVSRRAAQLCGAGMAAVVDKIRENRGLDHLDITVGVDGTLYKLHPHFSRIMHQTVKELAPKCNVTFLLSEDGSGKGAALITAVGCRLRQQEQKS

Target ORF information:

RefSeq Version	NM_213252.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hexokinase 1 (hk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213252.1

ORF Insert Sequence:

ATGATAGCGG CACAGCTTCT GGCCTACTAC TTCACTGAAC TGAAGGATGA CCAGGTCAAA 
AAGATTGACA AATACTTGTA CGCCATGCGG TTCTCTGACG AGACGCTGCG CGATATTATG 
GCTCGTTTCC GACGGGAAAT GGAGAACGGA TTGGCACGAG ACACCAACCC GACAGCCACC 
GCTAAAATGC TGCCCACCTT TGTAAGGTCC ATTCCTGATG GATCAGAGAA AGGAGATTTC 
ATCGCACTGG ATCTGGGTGG CTCTAATTTC CGTATTCTGC GTGTAAAAGT GTCTCATGAG 
AAGAAACAGA CAGTGCAGAT GGAAAGTCAG ATTTACGAGA CTCCAGAAGA CATCATCCAC 
GGCAGCAGAT CACGGTTGTT TGACCATGTT GCAGAATGTC TTGGAGACTT CATGGAGAAA 
CAAAAAATTA AAGACAAGAA GCTTCCTGTG GGCTTCACCT TCTCATTTCC TTGTTCACAA 
AGCAAACTGG ATGAGGCTGT TTTGCTGACA TGGACGAAAC GCTTCAAGGT TAATGGAGTA 
GAGGGAATGG ATGTAGTGAA GCTTCTCAAT AAGGCCATTA AAAAACGTGG AGATTACGAA 
GCAGATATAA TGGCTGTTGT CAATGACACT GTTGGAACTA TGATGACCTG CGGATTTGAT 
GACCAGCGAT GTGAAGTCGG CATTATCATA GGTACCGGCA CTAATGCATG CTACATGGAG 
GAGCTCAGAC ACATGGACAT GGTGGAGGGA GATGAAGGCA GGATGTGTAT TAATACTGAA 
TGGGGGGCGT TTGGAGATGA CGGCACATTG GAGGACATCC GGACTGAGTT TGACAGGGAG 
ATTGACCGAG GATCCCTCAA CCCTGGCAAG CAACTGTTTG AGAAGATGGT GAGTGGGATG 
TACATGGGTG AGCTGGTGAG GCTGATCCTG GTCAAAATGG CTAAAGAAGG CCTGCTGTTT 
GAAGGCCGCA TCACACCAGA GCTGCTTACA AAAGGAAAAA TCGAAACCAA GCACGTTTCT 
GCAATTGAGA AGAGCAAGGA AGGACTAACC AAAGCCAAGG AGATTTTGAC ACGCTTGGGT 
GTAGAGCCGT CTGAGGACGA CTGCATTGCT GTGCAGCATG TTTGTGCCAT TGTCTCTTTT 
CGCTCTGCTA ATCTAATTGC TGCCACTTTG GGTGCAATCC TGACACGTCT GAAGGACAAC 
AAGAACACCC CACGTCTCCG CACCACCGTG GGCATTGATG GCTCCCTATA CAAGATGCAC 
CCACAGTATG CCCGTCGGTT GCACAAAACA GTGCGTCGTC TGGTGCCAGA GTCTGATGTT 
CGCTTCTTGC TGTCTGAGAG TGGAAGTGGA AAGGGAGCTG CACTGGTGAC AGCCTGGGCT 
TACCGTTTGG CGGACCAGGA GCGTCAAATT GCAGAAACCC TGGAAGAGTT CAGGTTGACC 
AAAGACCAGC TGCTAGAGGT GAAAAAGAGG ATGAGAACTG AGATTCAGAA TGGACTGAGC 
AAGAGCACCC AAAACACAGC CACCGTCAAA ATGCTGCCCA CATATGTGCG GAGTACCCCT 
GATGGATCAG AAAACGGTGA CTTTTTGGCT CTGGATCTTG GAGGTACAAA CTTCAGGGTC 
CTTTTGGTAA AGATTCGCAG TGGCAAGAGA CGAACTGTGG AGATGCATAA TAAAATTTAT 
GCCATTCCCA TAGAAGTAAT GCAAGGAACC GGAGAGGAGC TTTTTGATCA CATCGTGTAC 
TGCATATCTG ACTTCCTGGA CTACATGGGA ATGAAGAATG CCCGTCTGCC TCTGGGCTTT 
ACCTTTTCTT TCCCCTGCAG ACAAACTAGT CTTGATGCGG GACTCTTGGT GAATTGGACG 
AAGGGCTTTA AAGCCACAGA CTGTGAAGGT GAAGATGTTG TCGGCCTTCT GAGAGAGGGG 
ATCAAGAGGA GAGAGGAGTT TGATTTGGAT GTTGTAGCCA TAGTAAATGA CACAGTGGGC 
ACAATGATGA CCTGCGCATA TGAAGAGCCC ACGTGTGAGG TTGGGCTAAT CGCAGGAACG 
GGCAGCAATG CCTGCTACAT GGAGGAAATG CGAAACATCG AGACAGTAAG TGGAGAGGAG 
GGCAGGATGT GTGTGAATAT GGAGTGGGGA GCATTTGGAG ATAATGGCTG TCTGGATGAC 
ATCCGGACGA AGTATGACGA TGCTGTGGAT GACCTCTCCC TCAATGCCGG CAAACAAAAG 
TATGAGAAGA TGTGCAGTGG CATGTACCTG GGTGAGATTG TTCGAAACAT CTTGATCGAC 
TTGACGAAGC GAGGCTTCCT CTTCAGGGGA CAGATCTCTG AAACACTGAA GACCAGAGGC 
ATCTTCGAAA CCAAGTTCCT GTCCCAAATT GAGAGTGACC GTTTGGCGCT ACTGCAGGTC 
AGGTCTATTC TCCAGCATCT GGGTCTGGAC AGCACATGCG ATGACAGTAT CATCGTCAAG 
GAGGTTTGTG GAGCTGTTTC TCGTCGAGCA GCTCAGCTCT GTGGAGCAGG AATGGCTGCT 
GTTGTGGATA AAATCCGGGA GAACCGTGGA CTGGATCATC TGGACATCAC TGTTGGAGTG 
GATGGCACCC TGTATAAGCT CCATCCACAT TTCTCTAGGA TAATGCATCA GACGGTGAAG 
GAGCTCGCCC CCAAATGCAA CGTCACTTTC CTTCTCTCAG AAGACGGAAG CGGTAAAGGT 
GCTGCCTTGA TCACCGCCGT TGGGTGTCGC CTCCGCCAGC AAGAACAGAA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The effects of Roundup® in embryo development and energy metabolism of the zebrafish (Danio rerio).
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP22274-81(2019 Aug)
Panetto OS,Gomes HF,Fraga Gomes DS,Campos E,Romeiro NC,Costa EP,do Carmo PRL,Feitosa NM,Moraes J

Toxic effects of boscalid in adult zebrafish (Danio rerio) on carbohydrate and lipid metabolism.
Environmental pollution (Barking, Essex : 1987)247775-782(2019 Apr)
Qian L,Zhang J,Chen X,Qi S,Wu P,Wang C,Wang C

Dietary administration of Bacillus amyloliquefaciens R8 reduces hepatic oxidative stress and enhances nutrient metabolism and immunity against Aeromonas hydrophila and Streptococcus agalactiae in zebrafish (Danio rerio).
Fish & shellfish immunology86410-419(2019 Mar)
Lin YS,Saputra F,Chen YC,Hu SY

Effects of polystyrene microplastics on the composition of the microbiome and metabolism in larval zebrafish.
Chemosphere217646-658(2019 Feb)
Wan Z,Wang C,Zhou J,Shen M,Wang X,Fu Z,Jin Y

Impairment of bisphenol F on the glucose metabolism of zebrafish larvae.
Ecotoxicology and environmental safety165386-392(2018 Dec)
Zhao F,Wang H,Wei P,Jiang G,Wang W,Zhang X,Ru S

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hk1 cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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