nrp2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nrp2a
Entrez Gene ID	405901
Full Name	neuropilin 2a
Synonyms	npn-2A
General protein information	Preferred Names neuropilin 2a Names neuropilin-2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212965.1	NP_998130.1	neuropilin-2
XM_021469092.1	XP_021324767.1	neuropilin-2 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	nrp2b	NP_998131.1
Homo sapiens (human)	NRP2	NP_957718.1
Bos taurus (cattle)	NRP2	NP_001180166.1
Xenopus tropicalis (tropical clawed frog)	nrp2	NP_001093705.1
Pan troglodytes (chimpanzee)	NRP2	XP_001136720.1
Mus musculus (house mouse)	Nrp2	NP_001070871.1
Canis lupus familiaris (dog)	NRP2	XP_005640627.1
Rattus norvegicus (Norway rat)	Nrp2	NP_110496.1
Gallus gallus (chicken)	NRP2	NP_989615.1
Danio rerio (zebrafish)	nrp2a	NP_998130.1
Macaca mulatta (Rhesus monkey)	NRP2	XP_002799071.1

Gene Ontology
Bibliography

Related articles in PubMed

Neuropilin 1 mediates epicardial activation and revascularization in the regenerating zebrafish heart.
Lowe V, Wisniewski L, Sayers J, Evans I, Frankel P, Mercader-Huber N, Zachary IC, Pellet-Many C
Development (Cambridge, England)146(13)(2019 Jul)

Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance.
van der Klaauw AA, Croizier S, Mendes de Oliveira E, Stadler LKJ, Park S, Kong Y, Banton MC, Tandon P, Hendricks AE, Keogh JM, Riley SE, Papadia S, Henning E, Bounds R, Bochukova EG, Mistry V, O'Rahilly S, Simerly RB, , , Minchin JEN, Barroso I, Jones EY, Bouret SG, Farooqi IS
Cell176(4)729-742.e18(2019 02)

HHEX is a transcriptional regulator of the VEGFC/FLT4/PROX1 signaling axis during vascular development.
Gauvrit S, Villasenor A, Strilic B, Kitchen P, Collins MM, Marín-Juez R, Guenther S, Maischein HM, Fukuda N, Canham MA, Brickman JM, Bogue CW, Jayaraman PS, Stainier DYR
Nature communications9(1)2704(2018 07)

Anti-angiogenic activity of water extract from Euphorbia pekinensis Rupr.
Zhang W, Liu B, Feng Y, Liu J, Ma Z, Zheng J, Xia Q, Ni Y, Li F, Lin R
Journal of ethnopharmacology206337-346(2017 Jul)

Mural lymphatic endothelial cells regulate meningeal angiogenesis in the zebrafish.
Bower NI, Koltowska K, Pichol-Thievend C, Virshup I, Paterson S, Lagendijk AK, Wang W, Lindsey BW, Bent SJ, Baek S, Rondon-Galeano M, Hurley DG, Mochizuki N, Simons C, Francois M, Wells CA, Kaslin J, Hogan BM
Nature neuroscience20(6)774-783(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nrp2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nrp2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01360	NM_212965.1 Latest version!	Danio rerio neuropilin 2a (nrp2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa147217	XM_021469092.1 Latest version!	Danio rerio neuropilin 2a (nrp2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nrp2a ( NM_212965.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998130.1 Danio rerio neuropilin 2a (nrp2a), mRNA.

Price & Availability↑

CloneID	ODa01360
Clone ID Related Accession (Same CDS sequence)	NM_212965.1
Accession Version	NM_212965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575648000000
Organism	Danio rerio(zebrafish)
Product	neuropilin-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY428801.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY428801.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATTTCT GGATATCACA CCAACGGATT GCATTTATCT TTTGCTTCGT TCTGAAAGTC 
AGCGCAAAAG AAAGTGAGAC ATGTGGAGGC CACCTGGATG CCAGCGGAGC CGGCTACATC 
ACATCTCCAG GATACCCTCT GGAATACCCG CCCCATCAGA GCTGTCAGTG GGTGATCAGC 
GCACCCGAAC CCTCACAGCG CATCGTGCTC AACTTCAACC CCCACTTTGA GCTGGAACGC 
CTGGACTGCA GGTATGACTT CATCGAGATC CGAGATGGGA ATTCGGAGTC TGCAGACCTG 
CTGGGCAAAC ACTGCAGTAA CATCGCTCCT CCCGCCATCA TCTCCTCCGG TCCCTTCATC 
CACATCAAGT TTGTGTCTGA TTACGCACAC CAGGGTGCCG GTTTCTCCCT GCGCTACGAG 
ATCTTCAAGA CTGGAGCTGA GCATTGTTTC CGTAATTTCA GTTCCCCAAC CGGAGTGATC 
GAGTCTCCGG GTTTCCCTGA CAAGTATCCA CATAATTTGG AGTGCTCCTT CATCATCATC 
GCTCCTCCTC AAACCGAGGT CACTCTCACC TTCCAGACCT TCGACCTGGA GAACGACCCT 
CTGCTGATGG GGGAAGGAGA GTGCAAATAT GACTGGCTCG ATGTCTGGGA CGGCTTGCCG 
CAAGTGGGAC CCTTGATCGG ACGATACTGT GGCTCGAAGA TCCCTCCAAA GATCCAGTCC 
TCCACGGGCC TTCTGTCGCT GTCCTTCCAC ACTGACATGG CTGTGGCCAA AGACGGATTC 
TCAGCCAGAT ATAACATGAC CCACAATGAA GTTAGCGACA CGTTTCACTG TAGTAATGCG 
TTTGGACTGG AGTCTGGGAA GATCTCTGAT GATCAGATTT CGGCCTCCAC TTCGTTTTAT 
GATGGACGCT GGCAGCCTCG TCAGGCACGT CTGAACAATG AGGACAACGC CTGGACGCCC 
GGTGAGGACA GCAACAAGGA GTACATACAG GTGGATCTTC AGTTCCTCAA GGTTCTGACC 
GGCATCGCTA CTCAAGGAGC CATTTCCAAA GAGACCCAGA AGTCATACTA CGTCACCACC 
TTCAAGCTGG AAGTTAGCAC TAACGGAGAG GACTGGATGT TTTATCGGCA TGGCAAAAAC 
CACAAGATTT TCCATGCCAA CACTGACCCG TCGGAGGTGG TTTTGAATCG TATCCCTCAG 
CCTGTTCTGG CCCGCTTTGT CCGCATTCGA CCACAGACGT GGAACAATGG CATTGCACTG 
CGCTTTGAGC TTTATGGCTG CCAGATTACA GATGCTCCTT GTTCAGAGCT CCAGGGAATG 
TTGTCAGGAC TCCTCCCGGA CTCCCAGATC TCAGCCTCCT CAATGCGGGA CCTCCACTGG 
AGCCCTGGAG CGGCTCGTTT GGTGGCCAGT CGATCAGGAT GGTTTCCCAG TCCTGCTCAG 
CCTATAGCTG GAGAAGAGTG GCTCCAGGTA GACCTCGGGA CACCCAGGAC GGTGCGTGGA 
GTGATAACTC AAGGAGCGCG GAGTGGTGAT GGAGGAACCA GCTCAGAGAA CCGGGCTTTC 
GTACGGAAGT ACCGCCTCGC ACACAGCCTG AACGGGAAAG ACTGGAACTT TGTAATAGAT 
AGCAAGACTA GCATGCCTAA GCTGTTTGAG GGGAACACGA ACTATGACAC CCCAGAGCTA 
CGTCGGTTTG AGGAGCTGCC AGCTCAGTTT ATCCGCATCT ATCCAGAGCG CTGGTCTCCG 
GCAGGCATCG GAATGAGAAT GGAAGTATTG GGCTGCAACC TGCCAGAAAC CTCCACACCA 
GCGCTGGAGA CGAGCAAACC AACACAACCG ACCTCAGTAG AGACCAGCAC GACACAGAAA 
CCCAGTCCAG TAACCAGCGC TGCCCTGCCA TCTCACAAAG GACTGTGTGA TTTTGAGCAG 
GGATGGTGTG GTTGGACCCT CGACCTGGAT TCTGACTTCA GCTGGGTTAT GCACAGCTCC 
AGCAAAAGCT CTGCCTTGGG TCAAAGTCAT AATCTCTCCA TGGGCTCAAA CAGTTTGGGA 
AACTACCTGT ACATCGAGGC TAGTCCAGAG ACGCAGGGCC AGAAAGCTCG CCTCTTCAGC 
CCGGGTGTGG ATCCAGACAC GGGACCCCTG TGCCTGATGT TCTCCTACCA GCTGGAGGAT 
CAGGGCACTC TGAGGGTCCT GCTGCAGAAC AAACAGCAGG AGAAGACGCT GCTGTGGTCC 
CTCAGCGGAG ACCAGCAGCC CATCTGGAGA GAAGGACGTA CCATTATTCC TCGATCCCCT 
GATGAGTTAA AGGTGGTAAT CGAAGGATTT TTCGAGGGTA CCAAAGGCCA CATTTGGATT 
GATAACATCC ACATGAGTGG GAACACACCG CTGGAAGAGT GCACTCAGCC GTTCTCCGCG 
TTTCCTCCGG ACATGATCGT TAAAAAACCA GAGGTTGGAA ATGGCCGTCT CTTCACCGGG 
AAGGACCACA TAAATGCAGG TTTCCAGTTT CCAGAGTGGG GCACGGCTGA GACCCCATCG 
GAGCCTCCGG TGACCCATGT GATCGATAAA GACAACTCAT GGCTGTACTC TCTGGACCCT 
ATTCTCCTGA CCATCATCGT CATGAGCTCT CTAGGAGTTC TTCTAGGTGC TGTTTGTGCT 
GGCCTACTCC TTTACTGCAC CTGCTCATAC GGAGGCTTAA CGTCTCGCTC TTCCACCACT 
TTGGAGAACT ACAACTTCGA GCTATACGAT GGGATTAAGC ACAAAGTGAA GCTCAACCAA 
CAGCGCTGCT GCTCCGAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998130.1
CDS	617..3400
Translation	MDFWISHQRIAFIFCFVLKVSAKESETCGGHLDASGAGYITSPGYPLEYPPHQSCQWVISAPEPSQRIVLNFNPHFELERLDCRYDFIEIRDGNSESADLLGKHCSNIAPPAIISSGPFIHIKFVSDYAHQGAGFSLRYEIFKTGAEHCFRNFSSPTGVIESPGFPDKYPHNLECSFIIIAPPQTEVTLTFQTFDLENDPLLMGEGECKYDWLDVWDGLPQVGPLIGRYCGSKIPPKIQSSTGLLSLSFHTDMAVAKDGFSARYNMTHNEVSDTFHCSNAFGLESGKISDDQISASTSFYDGRWQPRQARLNNEDNAWTPGEDSNKEYIQVDLQFLKVLTGIATQGAISKETQKSYYVTTFKLEVSTNGEDWMFYRHGKNHKIFHANTDPSEVVLNRIPQPVLARFVRIRPQTWNNGIALRFELYGCQITDAPCSELQGMLSGLLPDSQISASSMRDLHWSPGAARLVASRSGWFPSPAQPIAGEEWLQVDLGTPRTVRGVITQGARSGDGGTSSENRAFVRKYRLAHSLNGKDWNFVIDSKTSMPKLFEGNTNYDTPELRRFEELPAQFIRIYPERWSPAGIGMRMEVLGCNLPETSTPALETSKPTQPTSVETSTTQKPSPVTSAALPSHKGLCDFEQGWCGWTLDLDSDFSWVMHSSSKSSALGQSHNLSMGSNSLGNYLYIEASPETQGQKARLFSPGVDPDTGPLCLMFSYQLEDQGTLRVLLQNKQQEKTLLWSLSGDQQPIWREGRTIIPRSPDELKVVIEGFFEGTKGHIWIDNIHMSGNTPLEECTQPFSAFPPDMIVKKPEVGNGRLFTGKDHINAGFQFPEWGTAETPSEPPVTHVIDKDNSWLYSLDPILLTIIVMSSLGVLLGAVCAGLLLYCTCSYGGLTSRSSTTLENYNFELYDGIKHKVKLNQQRCCSEA

Target ORF information:

RefSeq Version	NM_212965.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuropilin 2a (nrp2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212965.1

ORF Insert Sequence:

ATGGATTTCT GGATATCACA CCAACGGATT GCATTTATCT TTTGCTTCGT TCTGAAAGTC 
AGCGCAAAAG AAAGTGAGAC ATGTGGAGGC CACCTGGATG CCAGCGGAGC CGGCTACATC 
ACATCTCCAG GATACCCTCT GGAATACCCG CCCCATCAGA GCTGTCAGTG GGTGATCAGC 
GCACCCGAAC CCTCACAGCG CATCGTGCTC AACTTCAACC CCCACTTTGA GCTGGAACGC 
CTGGACTGCA GGTATGACTT CATCGAGATC CGAGATGGGA ATTCGGAGTC TGCAGACCTG 
CTGGGCAAAC ACTGCAGTAA CATCGCTCCT CCCGCCATCA TCTCCTCCGG TCCCTTCATC 
CACATCAAGT TTGTGTCTGA TTACGCACAC CAGGGTGCCG GTTTCTCCCT GCGCTACGAG 
ATCTTCAAGA CTGGAGCTGA GCATTGTTTC CGTAATTTCA GTTCCCCAAC CGGAGTGATC 
GAGTCTCCGG GTTTCCCTGA CAAGTATCCA CATAATTTGG AGTGCTCCTT CATCATCATC 
GCTCCTCCTC AAACCGAGGT CACTCTCACC TTCCAGACCT TCGACCTGGA GAACGACCCT 
CTGCTGATGG GGGAAGGAGA GTGCAAATAT GACTGGCTCG ATGTCTGGGA CGGCTTGCCG 
CAAGTGGGAC CCTTGATCGG ACGATACTGT GGCTCGAAGA TCCCTCCAAA GATCCAGTCC 
TCCACGGGCC TTCTGTCGCT GTCCTTCCAC ACTGACATGG CTGTGGCCAA AGACGGATTC 
TCAGCCAGAT ATAACATGAC CCACAATGAA GTTAGCGACA CGTTTCACTG TAGTAATGCG 
TTTGGACTGG AGTCTGGGAA GATCTCTGAT GATCAGATTT CGGCCTCCAC TTCGTTTTAT 
GATGGACGCT GGCAGCCTCG TCAGGCACGT CTGAACAATG AGGACAACGC CTGGACGCCC 
GGTGAGGACA GCAACAAGGA GTACATACAG GTGGATCTTC AGTTCCTCAA GGTTCTGACC 
GGCATCGCTA CTCAAGGAGC CATTTCCAAA GAGACCCAGA AGTCATACTA CGTCACCACC 
TTCAAGCTGG AAGTTAGCAC TAACGGAGAG GACTGGATGT TTTATCGGCA TGGCAAAAAC 
CACAAGATTT TCCATGCCAA CACTGACCCG TCGGAGGTGG TTTTGAATCG TATCCCTCAG 
CCTGTTCTGG CCCGCTTTGT CCGCATTCGA CCACAGACGT GGAACAATGG CATTGCACTG 
CGCTTTGAGC TTTATGGCTG CCAGATTACA GATGCTCCTT GTTCAGAGCT CCAGGGAATG 
TTGTCAGGAC TCCTCCCGGA CTCCCAGATC TCAGCCTCCT CAATGCGGGA CCTCCACTGG 
AGCCCTGGAG CGGCTCGTTT GGTGGCCAGT CGATCAGGAT GGTTTCCCAG TCCTGCTCAG 
CCTATAGCTG GAGAAGAGTG GCTCCAGGTA GACCTCGGGA CACCCAGGAC GGTGCGTGGA 
GTGATAACTC AAGGAGCGCG GAGTGGTGAT GGAGGAACCA GCTCAGAGAA CCGGGCTTTC 
GTACGGAAGT ACCGCCTCGC ACACAGCCTG AACGGGAAAG ACTGGAACTT TGTAATAGAT 
AGCAAGACTA GCATGCCTAA GCTGTTTGAG GGGAACACGA ACTATGACAC CCCAGAGCTA 
CGTCGGTTTG AGGAGCTGCC AGCTCAGTTT ATCCGCATCT ATCCAGAGCG CTGGTCTCCG 
GCAGGCATCG GAATGAGAAT GGAAGTATTG GGCTGCAACC TGCCAGAAAC CTCCACACCA 
GCGCTGGAGA CGAGCAAACC AACACAACCG ACCTCAGTAG AGACCAGCAC GACACAGAAA 
CCCAGTCCAG TAACCAGCGC TGCCCTGCCA TCTCACAAAG GACTGTGTGA TTTTGAGCAG 
GGATGGTGTG GTTGGACCCT CGACCTGGAT TCTGACTTCA GCTGGGTTAT GCACAGCTCC 
AGCAAAAGCT CTGCCTTGGG TCAAAGTCAT AATCTCTCCA TGGGCTCAAA CAGTTTGGGA 
AACTACCTGT ACATCGAGGC TAGTCCAGAG ACGCAGGGCC AGAAAGCTCG CCTCTTCAGC 
CCGGGTGTGG ATCCAGACAC GGGACCCCTG TGCCTGATGT TCTCCTACCA GCTGGAGGAT 
CAGGGCACTC TGAGGGTCCT GCTGCAGAAC AAACAGCAGG AGAAGACGCT GCTGTGGTCC 
CTCAGCGGAG ACCAGCAGCC CATCTGGAGA GAAGGACGTA CCATTATTCC TCGATCCCCT 
GATGAGTTAA AGGTGGTAAT CGAAGGATTT TTCGAGGGTA CCAAAGGCCA CATTTGGATT 
GATAACATCC ACATGAGTGG GAACACACCG CTGGAAGAGT GCACTCAGCC GTTCTCCGCG 
TTTCCTCCGG ACATGATCGT TAAAAAACCA GAGGTTGGAA ATGGCCGTCT CTTCACCGGG 
AAGGACCACA TAAATGCAGG TTTCCAGTTT CCAGAGTGGG GCACGGCTGA GACCCCATCG 
GAGCCTCCGG TGACCCATGT GATCGATAAA GACAACTCAT GGCTGTACTC TCTGGACCCT 
ATTCTCCTGA CCATCATCGT CATGAGCTCT CTAGGAGTTC TTCTAGGTGC TGTTTGTGCT 
GGCCTACTCC TTTACTGCAC CTGCTCATAC GGAGGCTTAA CGTCTCGCTC TTCCACCACT 
TTGGAGAACT ACAACTTCGA GCTATACGAT GGGATTAAGC ACAAAGTGAA GCTCAACCAA 
CAGCGCTGCT GCTCCGAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrp2a ( XM_021469092.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324767.1 Danio rerio neuropilin 2a (nrp2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147217
Clone ID Related Accession (Same CDS sequence)	XM_021469092.1
Accession Version	XM_021469092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	neuropilin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTCT GGATATCACA CCAAGGGATT GCATTTATCT TTTGCTTCGT TCTGAAAGTC 
AGCGCAAAAG AAAGTGAGAC ATGTGGAGGC CACCTGGATG CCAGCGGAGC CGGCTACATC 
ACATCTCCAG GATACCCTCT GGAATACCCG CCCCATCAGA GCTGTCAGTG GGTGATCAGC 
GCACCCGAAC CCTCACAGCG CATCGTGCTC AACTTCAACC CCCACTTTGA GCTGGAACGC 
CTGGACTGCA GGTATGACTT CATCGAGATC CGAGATGGGA ATTCGGAGTC TGCAGACCTG 
CTGGGCAAAC ACTGCAGTAA CATCGCTCCT CCCGCCATCA TCTCCTCCGG TCCCTTCATC 
CACATCAAGT TTGTGTCTGA TTACGCACAC CAGGGTGCCG GTTTCTCCCT GCGCTACGAG 
ATCTTCAAGA CTGGAGCTGA GCATTGTTTC CGTAATTTCA GTTCCCCAAC CGGAGTGATC 
GAGTCTCCGG GTTTCCCTGA CAAGTATCCA CATAATTTGG AGTGCTCCTT CATCATCATC 
GCTCCTCCTC AAACCGAGGT CACTCTCACC TTCCAGACCT TCGACCTGGA GAACGACCCT 
CTGCTGATGG GGGAAGGAGA GTGCAAATAC GACTGGCTCG ATGTCTGGGA CGGCCTGCCG 
CAAGTGGGAC CCTTGATCGG ACGATACTGT GGCTCGAAGA TCCCTCCAAA GATCCAGTCC 
TCCACGGGCC TTCTGTCGCT GTCCTTCCAC ACTGACATGG CTGTGGCCAA AGACGGATTC 
TCGGCCAGAT ATAACATGAC CCACAAGGAA GTTAGCGACA CGTTTCACTG TAGTAATGCG 
TTTGGACTGG AGTCTGGGAA GATCTCTGAT GATCAGATTT CGGCCTCCAC TTCGTTTTAT 
GATGGACGCT GGCAGCCTCG TCAGGCACGT CTGAACAATG AGGACAACGC CTGGACGCCC 
GGTGAGGACA GCAACAAGGA GTACATACAG GTGGATCTTC AGTTCCTCAA GGTTCTGACC 
GGCATCGCTA CTCAAGGAGC CATTTCCAAA GAGACCCAGA AGTCATACTA CGTCACCACC 
TTCAAGCTGG AAGTTAGCAC TAACGGAGAG GACTGGATGT TTTATCGGCA TGGCAAAAAC 
CACAAGATTT TCCATGCCAA CACTGACCCG TCGGAGGTGG TTTTGAATCG TATCCCTCAG 
CCTGTTCTGG CCCGCTTTGT CCGCATTCGA CCACAGACGT GGAACAATGG CATTGCACTG 
CGCTTTGAGC TTTATGGCTG CCAGATTACA GATGCTCCTT GTTCAGAGCT CCAGGGAATG 
TTGTCAGGAC TCCTCCCGGA CTCCCAGATC TCAGCCTCCT CAATGCGGGA CCTCCACTGG 
AGCCCTGGAG CGGCTCGTTT GGTGGCCAGT CGATCAGGAT GGTTTCCCAG TCCTGCTCAG 
CCTATAGCTG GAGAAGAGTG GCTCCAGGTA GACCTCGGGA CACCCCGGAT GGTGCGTGGA 
GTGATAACTC AAGGAGCGCG GAGTGGTGAT GGAGGAACCA GCTCAGAGAA CCGGGCTTTC 
GTACGGAAGT ACCGCCTCGC ACACAGCCTG AACGGGAAAG ACTGGAACTT TGTAATAGAT 
AGCAAGACTA GCATGCCTAA GCTGTTTGAG GGGAACACGA ACTACGACAC CCCAGAGCTG 
CGTCGGTTTG AGGAGCTGCC AGCTCAGTTT ATCCGCATCT ATCCAGAGCG CTGGTCTCCG 
GCAGGCATCG GAATGAGAAT GGAAGTATTG GGCTGCAACC TGCCAGAAAC CTCCACACCA 
GCGCTGGAGA CGAGCAAACC AACACAACCG ACCTCAGTAG AGACCAGCAC GACACAGAAA 
CCCAGTCCAG TAACCAGCGC TGCCCTGCCG TCTCACAAAG GACTGTGTGA TTTTGAGCAG 
GGATGGTGTG GTTGGACCCT CGACCTGGAT TCTGACTTCA GCTGGGTTAT GCACAGCTCC 
AGCAAAAGCT CTGCCTTGGG TCAAAGTCAT AATCTCTCCA TGGGCTCAAA CAGTTTGGGA 
AACTACCTGT ACATCGAGGC TAGTCCAGAG ACGCAGGGCC AGAAAGCTCG CCTCTTCAGC 
CCGGGTGTGG ATCCAGACAC GGGACCCCTG TGCCTGATGT TCTCCTACCA GCTGGAGGAT 
CAGGGCACTC TGAGGGTCCT GCTGCAGAAC AAACAGCAGG AGAAGACGCT GCTGTGGTCC 
CTCAGCGGAG ACCAGCAGCC CATCTGGAGA GAAGGACGTA CCATTATTCC TCGATCCCCT 
GATGAGTTAA AGGTGGTAAT CGAAGGATTT TTCGAGGGTA CCAAAGGCCA CATATGGATT 
GATAACATTC ACATGAGTGG GAACACACCG CTGGAAGAGT GCACTCAGCC GTTCTCCGCG 
TTTCCTCCGG ACATGATCGG GGGAGCCCTA CCTGGCAAGA GTGAGCCAAC AATAGATACG 
CTGTCAATAC CGCCCATTTT GCCCAACTGG TATTACATAA TTGCTGGTGG AGGAGCCATC 
TTGTTCCTGG TCATTGTCAT TGTCATGGCC ATTTGCTGTC GTCATCTTAC GGCCAGGAGG 
AACCAACCTA CAATGGCTTA CTACGGTCCT CACAATTCAC ACTATCCCAA CGGCGGCATT 
CACAGGCCTG GGACAGCGCC CAAATTGACT GTATGGGTCG AGAAGGGTGA TGGGAACTCC 
TACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324767.1
CDS	637..3345
Translation	MDFWISHQGIAFIFCFVLKVSAKESETCGGHLDASGAGYITSPGYPLEYPPHQSCQWVISAPEPSQRIVLNFNPHFELERLDCRYDFIEIRDGNSESADLLGKHCSNIAPPAIISSGPFIHIKFVSDYAHQGAGFSLRYEIFKTGAEHCFRNFSSPTGVIESPGFPDKYPHNLECSFIIIAPPQTEVTLTFQTFDLENDPLLMGEGECKYDWLDVWDGLPQVGPLIGRYCGSKIPPKIQSSTGLLSLSFHTDMAVAKDGFSARYNMTHKEVSDTFHCSNAFGLESGKISDDQISASTSFYDGRWQPRQARLNNEDNAWTPGEDSNKEYIQVDLQFLKVLTGIATQGAISKETQKSYYVTTFKLEVSTNGEDWMFYRHGKNHKIFHANTDPSEVVLNRIPQPVLARFVRIRPQTWNNGIALRFELYGCQITDAPCSELQGMLSGLLPDSQISASSMRDLHWSPGAARLVASRSGWFPSPAQPIAGEEWLQVDLGTPRMVRGVITQGARSGDGGTSSENRAFVRKYRLAHSLNGKDWNFVIDSKTSMPKLFEGNTNYDTPELRRFEELPAQFIRIYPERWSPAGIGMRMEVLGCNLPETSTPALETSKPTQPTSVETSTTQKPSPVTSAALPSHKGLCDFEQGWCGWTLDLDSDFSWVMHSSSKSSALGQSHNLSMGSNSLGNYLYIEASPETQGQKARLFSPGVDPDTGPLCLMFSYQLEDQGTLRVLLQNKQQEKTLLWSLSGDQQPIWREGRTIIPRSPDELKVVIEGFFEGTKGHIWIDNIHMSGNTPLEECTQPFSAFPPDMIGGALPGKSEPTIDTLSIPPILPNWYYIIAGGGAILFLVIVIVMAICCRHLTARRNQPTMAYYGPHNSHYPNGGIHRPGTAPKLTVWVEKGDGNSYW

Target ORF information:

RefSeq Version	XM_021469092.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuropilin 2a (nrp2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469092.1

ORF Insert Sequence:

ATGGATTTCT GGATATCACA CCAAGGGATT GCATTTATCT TTTGCTTCGT TCTGAAAGTC 
AGCGCAAAAG AAAGTGAGAC ATGTGGAGGC CACCTGGATG CCAGCGGAGC CGGCTACATC 
ACATCTCCAG GATACCCTCT GGAATACCCG CCCCATCAGA GCTGTCAGTG GGTGATCAGC 
GCACCCGAAC CCTCACAGCG CATCGTGCTC AACTTCAACC CCCACTTTGA GCTGGAACGC 
CTGGACTGCA GGTATGACTT CATCGAGATC CGAGATGGGA ATTCGGAGTC TGCAGACCTG 
CTGGGCAAAC ACTGCAGTAA CATCGCTCCT CCCGCCATCA TCTCCTCCGG TCCCTTCATC 
CACATCAAGT TTGTGTCTGA TTACGCACAC CAGGGTGCCG GTTTCTCCCT GCGCTACGAG 
ATCTTCAAGA CTGGAGCTGA GCATTGTTTC CGTAATTTCA GTTCCCCAAC CGGAGTGATC 
GAGTCTCCGG GTTTCCCTGA CAAGTATCCA CATAATTTGG AGTGCTCCTT CATCATCATC 
GCTCCTCCTC AAACCGAGGT CACTCTCACC TTCCAGACCT TCGACCTGGA GAACGACCCT 
CTGCTGATGG GGGAAGGAGA GTGCAAATAC GACTGGCTCG ATGTCTGGGA CGGCCTGCCG 
CAAGTGGGAC CCTTGATCGG ACGATACTGT GGCTCGAAGA TCCCTCCAAA GATCCAGTCC 
TCCACGGGCC TTCTGTCGCT GTCCTTCCAC ACTGACATGG CTGTGGCCAA AGACGGATTC 
TCGGCCAGAT ATAACATGAC CCACAAGGAA GTTAGCGACA CGTTTCACTG TAGTAATGCG 
TTTGGACTGG AGTCTGGGAA GATCTCTGAT GATCAGATTT CGGCCTCCAC TTCGTTTTAT 
GATGGACGCT GGCAGCCTCG TCAGGCACGT CTGAACAATG AGGACAACGC CTGGACGCCC 
GGTGAGGACA GCAACAAGGA GTACATACAG GTGGATCTTC AGTTCCTCAA GGTTCTGACC 
GGCATCGCTA CTCAAGGAGC CATTTCCAAA GAGACCCAGA AGTCATACTA CGTCACCACC 
TTCAAGCTGG AAGTTAGCAC TAACGGAGAG GACTGGATGT TTTATCGGCA TGGCAAAAAC 
CACAAGATTT TCCATGCCAA CACTGACCCG TCGGAGGTGG TTTTGAATCG TATCCCTCAG 
CCTGTTCTGG CCCGCTTTGT CCGCATTCGA CCACAGACGT GGAACAATGG CATTGCACTG 
CGCTTTGAGC TTTATGGCTG CCAGATTACA GATGCTCCTT GTTCAGAGCT CCAGGGAATG 
TTGTCAGGAC TCCTCCCGGA CTCCCAGATC TCAGCCTCCT CAATGCGGGA CCTCCACTGG 
AGCCCTGGAG CGGCTCGTTT GGTGGCCAGT CGATCAGGAT GGTTTCCCAG TCCTGCTCAG 
CCTATAGCTG GAGAAGAGTG GCTCCAGGTA GACCTCGGGA CACCCCGGAT GGTGCGTGGA 
GTGATAACTC AAGGAGCGCG GAGTGGTGAT GGAGGAACCA GCTCAGAGAA CCGGGCTTTC 
GTACGGAAGT ACCGCCTCGC ACACAGCCTG AACGGGAAAG ACTGGAACTT TGTAATAGAT 
AGCAAGACTA GCATGCCTAA GCTGTTTGAG GGGAACACGA ACTACGACAC CCCAGAGCTG 
CGTCGGTTTG AGGAGCTGCC AGCTCAGTTT ATCCGCATCT ATCCAGAGCG CTGGTCTCCG 
GCAGGCATCG GAATGAGAAT GGAAGTATTG GGCTGCAACC TGCCAGAAAC CTCCACACCA 
GCGCTGGAGA CGAGCAAACC AACACAACCG ACCTCAGTAG AGACCAGCAC GACACAGAAA 
CCCAGTCCAG TAACCAGCGC TGCCCTGCCG TCTCACAAAG GACTGTGTGA TTTTGAGCAG 
GGATGGTGTG GTTGGACCCT CGACCTGGAT TCTGACTTCA GCTGGGTTAT GCACAGCTCC 
AGCAAAAGCT CTGCCTTGGG TCAAAGTCAT AATCTCTCCA TGGGCTCAAA CAGTTTGGGA 
AACTACCTGT ACATCGAGGC TAGTCCAGAG ACGCAGGGCC AGAAAGCTCG CCTCTTCAGC 
CCGGGTGTGG ATCCAGACAC GGGACCCCTG TGCCTGATGT TCTCCTACCA GCTGGAGGAT 
CAGGGCACTC TGAGGGTCCT GCTGCAGAAC AAACAGCAGG AGAAGACGCT GCTGTGGTCC 
CTCAGCGGAG ACCAGCAGCC CATCTGGAGA GAAGGACGTA CCATTATTCC TCGATCCCCT 
GATGAGTTAA AGGTGGTAAT CGAAGGATTT TTCGAGGGTA CCAAAGGCCA CATATGGATT 
GATAACATTC ACATGAGTGG GAACACACCG CTGGAAGAGT GCACTCAGCC GTTCTCCGCG 
TTTCCTCCGG ACATGATCGG GGGAGCCCTA CCTGGCAAGA GTGAGCCAAC AATAGATACG 
CTGTCAATAC CGCCCATTTT GCCCAACTGG TATTACATAA TTGCTGGTGG AGGAGCCATC 
TTGTTCCTGG TCATTGTCAT TGTCATGGCC ATTTGCTGTC GTCATCTTAC GGCCAGGAGG 
AACCAACCTA CAATGGCTTA CTACGGTCCT CACAATTCAC ACTATCCCAA CGGCGGCATT 
CACAGGCCTG GGACAGCGCC CAAATTGACT GTATGGGTCG AGAAGGGTGA TGGGAACTCC 
TACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neuropilin 1 mediates epicardial activation and revascularization in the regenerating zebrafish heart.
Development (Cambridge, England)146(13)(2019 Jul)
Lowe V,Wisniewski L,Sayers J,Evans I,Frankel P,Mercader-Huber N,Zachary IC,Pellet-Many C

Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance.
Cell176(4)729-742.e18(2019 02)
van der Klaauw AA,Croizier S,Mendes de Oliveira E,Stadler LKJ,Park S,Kong Y,Banton MC,Tandon P,Hendricks AE,Keogh JM,Riley SE,Papadia S,Henning E,Bounds R,Bochukova EG,Mistry V,O'Rahilly S,Simerly RB,,,Minchin JEN,Barroso I,Jones EY,Bouret SG,Farooqi IS

HHEX is a transcriptional regulator of the VEGFC/FLT4/PROX1 signaling axis during vascular development.
Nature communications9(1)2704(2018 07)
Gauvrit S,Villasenor A,Strilic B,Kitchen P,Collins MM,Marín-Juez R,Guenther S,Maischein HM,Fukuda N,Canham MA,Brickman JM,Bogue CW,Jayaraman PS,Stainier DYR

Anti-angiogenic activity of water extract from Euphorbia pekinensis Rupr.
Journal of ethnopharmacology206337-346(2017 Jul)
Zhang W,Liu B,Feng Y,Liu J,Ma Z,Zheng J,Xia Q,Ni Y,Li F,Lin R

Mural lymphatic endothelial cells regulate meningeal angiogenesis in the zebrafish.
Nature neuroscience20(6)774-783(2017 Jun)
Bower NI,Koltowska K,Pichol-Thievend C,Virshup I,Paterson S,Lagendijk AK,Wang W,Lindsey BW,Bent SJ,Baek S,Rondon-Galeano M,Hurley DG,Mochizuki N,Simons C,Francois M,Wells CA,Kaslin J,Hogan BM

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nrp2a cDNA ORF clone, Danio rerio(zebrafish)

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