tnfa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tnfa
Entrez Gene ID	405785
Full Name	tumor necrosis factor a (TNF superfamily, member 2)
General protein information	Preferred Names tumor necrosis factor a (TNF superfamily, member 2) Names tumor necrosis factor, alpha TNF-alpha 1 TNF1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212859.2	NP_998024.2	tumor necrosis factor, alpha

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	tnfa	NP_998024.2
Danio rerio (zebrafish)	tnfb	NP_001019618.1

Gene Ontology
Bibliography

Related articles in PubMed

Zebrafish modeling of intestinal injury, bacterial exposures and medications defines epithelial
Chuang LS, Morrison J, Hsu NY, Labrias PR, Nayar S, Chen E, Villaverde N, Facey JA, Boschetti G, Giri M, Castillo-Martin M, Thin TH, Sharma Y, Chu J, Cho JH
Disease models & mechanisms12(8)(2019 08)

The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection.
Yang D, Liu X, Xu W, Gu Z, Yang C, Zhang L, Tan J, Zheng X, Wang Z, Quan S, Zhang Y, Liu Q
PLoS pathogens15(7)e1007917(2019 07)

What makes a bad egg? Egg transcriptome reveals dysregulation of translational machinery and novel fertility genes important for fertilization.
Cheung CT, Nguyen TV, Le Cam A, Patinote A, Journot L, Reynes C, Bobe J
BMC genomics20(1)584(2019 Jul)

Differential Apoptotic and Mitogenic Effects of Lectins in Zebrafish.
Wang K, Liu C, Hou Y, Zhou H, Wang X, Mai K, He G
Frontiers in endocrinology10356(2019)

Knockout of longevity gene Sirt1 in zebrafish leads to oxidative injury, chronic inflammation, and reduced life span.
Kim DH, Jung IH, Kim DH, Park SW
PloS one14(8)e0220581(2019)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tnfa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tnfa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01539	NM_212859.2 Latest version!	Danio rerio tumor necrosis factor a (TNF superfamily, member 2) (tnfa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tnfa ( NM_212859.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998024.2 Danio rerio tumor necrosis factor a (TNF superfamily, member 2) (tnfa), mRNA.

Price & Availability↑

CloneID	ODa01539
Clone ID Related Accession (Same CDS sequence)	NM_212859.2
Accession Version	NM_212859.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1578067200000
Organism	Danio rerio(zebrafish)
Product	tumor necrosis factor, alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124141.1. On Oct 1, 2006 this sequence version replaced NM_212859.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC124141.1, DV593921.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCTTG AGAGTCGGGC GCTTTTGGAT GTTGAAGAAG GAGAGTTGCC TTTACCGCTG 
GTGATGGTGT CTAGGAGGAA AGCTGGATCT TCAAAGTCGG GTGTATGGAG GGTGTTTGGG 
ATCATTTTGG CTGTGGGCCT TTGTGCCGCT GCTGCCGTCT GCTTCACGCT CCATAAGACC 
CAGGGCAATC AACAAGATGG AAGTGTGCTG AGACTCACAT TGAGAGATCG CATTTCACAA 
GGCAATTTCA CTTCCAAGGC TGCCATCCAT TTAACAGGTG GATACAACTC TGAATCAAAG 
ACCTTAGACT GGAGAGATGA CCAGGACCAG GCCTTTTCTT CAGGTGGCTT GAAATTAGTA 
AACAGGGAGA TTATCATTCC CGATGATGGC ATTTATTTTG TCTACAGCCA GGTGTCTTTG 
CACATCAGCT GCACGTCTGA ACTGACTGAG GAACAAGTGC TTATGAGCCA TGCAGTGATG 
CGCTTTTCTG AATCCTACGG AGGCAAAAAG CCACTTTTCA GTGCAATCCG CTCAATCTGC 
ACGCAGGAGC CTGAATCTGA AAATCTGTGG TACAACACTA TTTACCTCGG CGCTGCCTTC 
CATTTACGAG AAGGAGACAG ACTGGGCACA GACACGACCA CAGCACTTCT ACCGATGGTT 
GAAAATGATA ACGGAAAGAC CTTCTTTGGG GTGTTTGGTT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998024.2
CDS	98..802
Translation	MKLESRALLDVEEGELPLPLVMVSRRKAGSSKSGVWRVFGIILAVGLCAAAAVCFTLHKTQGNQQDGSVLRLTLRDRISQGNFTSKAAIHLTGGYNSESKTLDWRDDQDQAFSSGGLKLVNREIIIPDDGIYFVYSQVSLHISCTSELTEEQVLMSHAVMRFSESYGGKKPLFSAIRSICTQEPESENLWYNTIYLGAAFHLREGDRLGTDTTTALLPMVENDNGKTFFGVFGL

Target ORF information:

RefSeq Version	NM_212859.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tumor necrosis factor a (TNF superfamily, member 2) (tnfa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212859.2

ORF Insert Sequence:

ATGAAGCTTG AGAGTCGGGC GCTTTTGGAT GTTGAAGAAG GAGAGTTGCC TTTACCGCTG 
GTGATGGTGT CTAGGAGGAA AGCTGGATCT TCAAAGTCGG GTGTATGGAG GGTGTTTGGG 
ATCATTTTGG CTGTGGGCCT TTGTGCCGCT GCTGCCGTCT GCTTCACGCT CCATAAGACC 
CAGGGCAATC AACAAGATGG AAGTGTGCTG AGACTCACAT TGAGAGATCG CATTTCACAA 
GGCAATTTCA CTTCCAAGGC TGCCATCCAT TTAACAGGTG GATACAACTC TGAATCAAAG 
ACCTTAGACT GGAGAGATGA CCAGGACCAG GCCTTTTCTT CAGGTGGCTT GAAATTAGTA 
AACAGGGAGA TTATCATTCC CGATGATGGC ATTTATTTTG TCTACAGCCA GGTGTCTTTG 
CACATCAGCT GCACGTCTGA ACTGACTGAG GAACAAGTGC TTATGAGCCA TGCAGTGATG 
CGCTTTTCTG AATCCTACGG AGGCAAAAAG CCACTTTTCA GTGCAATCCG CTCAATCTGC 
ACGCAGGAGC CTGAATCTGA AAATCTGTGG TACAACACTA TTTACCTCGG CGCTGCCTTC 
CATTTACGAG AAGGAGACAG ACTGGGCACA GACACGACCA CAGCACTTCT ACCGATGGTT 
GAAAATGATA ACGGAAAGAC CTTCTTTGGG GTGTTTGGTT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zebrafish modeling of intestinal injury, bacterial exposures and medications defines epithelial
Disease models & mechanisms12(8)(2019 08)
Chuang LS,Morrison J,Hsu NY,Labrias PR,Nayar S,Chen E,Villaverde N,Facey JA,Boschetti G,Giri M,Castillo-Martin M,Thin TH,Sharma Y,Chu J,Cho JH

The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection.
PLoS pathogens15(7)e1007917(2019 07)
Yang D,Liu X,Xu W,Gu Z,Yang C,Zhang L,Tan J,Zheng X,Wang Z,Quan S,Zhang Y,Liu Q

What makes a bad egg? Egg transcriptome reveals dysregulation of translational machinery and novel fertility genes important for fertilization.
BMC genomics20(1)584(2019 Jul)
Cheung CT,Nguyen TV,Le Cam A,Patinote A,Journot L,Reynes C,Bobe J

Differential Apoptotic and Mitogenic Effects of Lectins in Zebrafish.
Frontiers in endocrinology10356(2019)
Wang K,Liu C,Hou Y,Zhou H,Wang X,Mai K,He G

Knockout of longevity gene Sirt1 in zebrafish leads to oxidative injury, chronic inflammation, and reduced life span.
PloS one14(8)e0220581(2019)
Kim DH,Jung IH,Kim DH,Park SW