prpf3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prpf3
Entrez Gene ID	403072
Full Name	PRP3 pre-mRNA processing factor 3 homolog (yeast)
Synonyms	HPRP3,HPRP3P,wu:fa11f05
General protein information	Preferred Names PRP3 pre-mRNA processing factor 3 homolog (yeast) Names U4/U6 small nuclear ribonucleoprotein Prp3 U4/U6-associated RNA splicing factor
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205748.2	NP_991311.2	U4/U6 small nuclear ribonucleoprotein Prp3

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os09g0249600	NP_001062673.1
Pan troglodytes (chimpanzee)	PRPF3	NP_001233355.1
Caenorhabditis elegans (roundworm)	prp-3	NP_499300.1
Homo sapiens (human)	PRPF3	NP_004689.1
Macaca mulatta (Rhesus monkey)	PRPF3	XP_001101105.1
Mus musculus (house mouse)	Prpf3	NP_081817.2
Bos taurus (cattle)	PRPF3	NP_001039516.1
Gallus gallus (chicken)	PRPF3	NP_001026561.1
Danio rerio (zebrafish)	prpf3	NP_991311.1
Xenopus tropicalis (tropical clawed frog)	prpf3	NP_001107672.1
Neurospora crassa	NCU01637	XP_956005.1
Canis lupus familiaris (dog)	PRPF3	XP_003639679.1
Schizosaccharomyces pombe (fission yeast)	prp3	NP_594560.1
Drosophila melanogaster (fruit fly)	Prp3	NP_649156.1
Magnaporthe oryzae (rice blast fungus)	MGG_01157	XP_003717764.1
Rattus norvegicus (Norway rat)	Prpf3	NP_001102029.1
Arabidopsis thaliana (thale cress)	AT1G28060	NP_174127.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004682	XP_307259.4

Gene Ontology
Bibliography

Related articles in PubMed

Knocking Down Snrnp200 Initiates Demorphogenesis of Rod Photoreceptors in Zebrafish.
Liu Y, Chen X, Qin B, Zhao K, Zhao Q, Staley JP, Zhao C
Journal of ophthalmology2015816329(2015)

PRPF4 mutations cause autosomal dominant retinitis pigmentosa.
Chen X, Liu Y, Sheng X, Tam PO, Zhao K, Chen X, Rong W, Liu Y, Liu X, Pan X, Chen LJ, Zhao Q, Vollrath D, Pang CP, Zhao C
Human molecular genetics23(11)2926-39(2014 Jun)

Sperm, but not oocyte, DNA methylome is inherited by zebrafish early embryos.
Jiang L, Zhang J, Wang JJ, Wang L, Zhang L, Li G, Yang X, Ma X, Sun X, Cai J, Zhang J, Huang X, Yu M, Wang X, Liu F, Wu CI, He C, Zhang B, Ci W, Liu J
Cell153(4)773-84(2013 May)

Decreased levels of the RNA splicing factor Prpf3 in mice and zebrafish do not cause photoreceptor degeneration.
Graziotto JJ, Inglehearn CF, Pack MA, Pierce EA
Investigative ophthalmology & visual science49(9)3830-8(2008 Sep)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following prpf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prpf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa40764	NM_205748.2 Latest version!	Danio rerio PRP3 pre-mRNA processing factor 3 homolog (yeast) (prpf3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prpf3 ( NM_205748.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991311.2 Danio rerio PRP3 pre-mRNA processing factor 3 homolog (yeast) (prpf3), mRNA.

Price & Availability↑

CloneID	ODa40764
Clone ID Related Accession (Same CDS sequence)	NM_205748.2
Accession Version	NM_205748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Danio rerio(zebrafish)
Product	U4/U6 small nuclear ribonucleoprotein Prp3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC139603.1. On Jun 19, 2015 this sequence version replaced NM_205748.1. ##Evidence-Data-START## Transcript exon combination :: BC139603.1, AY648801.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCACTGC CTAAACGTGA GGTTGATGAA CTGAGGCCAT GGGTGGAACG CACAGTAAAG 
AAGGTCCTTG GCTTCTCTGA ACCCACTGTG GTCACAGCAG CATTGCATTG TGTGGGAAAA 
GGCTTAGACA AAAGAAAAAC CATAGATCAG CTAAGACCAT TTCTTGATGA TTCGGCAAGC 
AGTTTCGTTG AGCGTCTGTT TGAAGCTCTC GAGGAAAGCA GAAACTCTCG TGGAAGTAAA 
GGAAGTGGAG AGAAAAACCG CAAAAGAGAA CTAAAAGAAG TGTTTGGAGA TGAGATGGAA 
GGGGTGAAGG ATGTAGTCAG TGTGGAATCA GCAGTAAAAA AGAAAAGGGT GCCCCGCTTT 
GAGGAGGTGG AAGAACCAGT TGTTTTACCT GGTCCCCCGA CTGAGAGTCC TGGCATGCTC 
ACTAAGATTC AGATAAAGCA AATGATGGAA GCAGCCACAC GACAGATTGA GGAGAGAAAG 
AAACAGCTAA GTTTTGCGGC TCCTACCCCC TCTTCGCAGT CAAGATTAAG TGCTTCTCAG 
ACAGACTCTA GTACTCCACG ATCTATGAAC CAAGCAGGGG GTGCAACCCA GTCCATCGCT 
CCTTCTCAGG CTGCTAATTT CATGAATGAT GCTATAGAAA AAGCACGCAA GGCAGCAGAG 
CTGCAGGCCA AGATCCAATC TACACTGTCC ATGAAACCAG GAATTCTGGC TACTGTGGGA 
AACCCTGGGC CACATAATAT GGTAGCACTG GCCAACCTAC ACGCCATGGG CATTGCACCA 
CCTAAAGTCG AACCTCGCGA GATCACAAAG CCAACACCTC TCATTTTGGA TGAGCTCGGA 
CGAACTGTTG ATGCCAGTGG GAAAGAAATT GAGCTCACAC ATCGTATGCC CACTCTCAAA 
GCAAACATCA GAGCTGTGAA GAGGGAACAG TTTCGACAAC AGTTAAAAGA AAAGCCTAGC 
GATGACCTAG AATCCACATC TTACTTTGAC CCACGTGTGG CCCTTGCCCC TGCACTAAAA 
CCCAAAAAAG GCTTTAGGTT CCATGAACAG GGGCGCTTTG AGAAGATAGC CCAGCGCATA 
CGCACCAAAG CTCAGCTGGA AAAATTACAA ATGGAGATTG CTCAGGCTGC CAAAAAGACT 
GGAATACAGG CCTCCACTAA ACTGGCTCTT ATTGCCCCAA AGAAGGAGCT GGGAGAGGGG 
GAGATTCCTA ATATAGAGTG GTGGGACTCC TACATACTGC CCTTCCATAT AAACCTATCT 
GCACATACAA ATTTAGATGA GGTGGAAATG CATGGAGTCA CCAACCTAGT TGAACATCCA 
ACTCAGATGG CTCCACCAGT GGATACCGAC AAGCCAGTTA CTCTTGGTGT GTATTTGACT 
AAGAAAGAAC AGAAGAAGTT GAGGAGACAA ACTCGCAGAG AAGGCCAGAA AGAGGTGCAG 
GAAAAAGTCA GATTAGGCCT TATGCCTCCA CCTGAACCCA AAGTGCGGAT CTCCAATCTG 
ATGAGGGTTT TGGGCACAGA AGCTGTGCAG GGCCCCACTA AAGTGGAGGC CCATGTTAGA 
GCACAAATGG CAAAGAGGCA GAAGGCACAT GAGGAGGCAA ATGCAGCACG CAAACTCACA 
GCAGAACAAA GGAAAGAGAA GAAGGTGAAA AAACTGAAGG AGGATTTGAG TCATGGAGTT 
CATATTGCTG TTTACAGAAT CCGAAATCTG CATAACCCAG CCAAGAAGTT TAAAGTGGAG 
GCCAATGCAA ACCAGCTGTA CCTCACTGGC ACTGTGGTAC TGCATAAAGA TGTCAACATC 
GTAGTTGTGG AAGGAGGACC CAAAGCACAG AAGAAGTTTA AGCGATTGAT GCTGACTCGG 
ATCAAATGGG ATGAGCCAAA TTCCAAAAGA GATGAGCATC ACGATGACGA TGAATCCAGG 
AGGAATAATA GATGCTCATT GGTCTGGGAG GGTACATCAA AAGAACGTAA CTATGGGGAG 
GTTAAGTTTA AGCAGTGTCC AACGGAGAAC ATGGCTCGGG AACATTTTAA GAAGCATGGC 
ACCGAGCACT ACTGGGACTT GGCTCTGAGC CAAAGTGTTC TGGAGACAAC TGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991311.2
CDS	182..2281
Translation	MSLPKREVDELRPWVERTVKKVLGFSEPTVVTAALHCVGKGLDKRKTIDQLRPFLDDSASSFVERLFEALEESRNSRGSKGSGEKNRKRELKEVFGDEMEGVKDVVSVESAVKKKRVPRFEEVEEPVVLPGPPTESPGMLTKIQIKQMMEAATRQIEERKKQLSFAAPTPSSQSRLSASQTDSSTPRSMNQAGGATQSIAPSQAANFMNDAIEKARKAAELQAKIQSTLSMKPGILATVGNPGPHNMVALANLHAMGIAPPKVEPREITKPTPLILDELGRTVDASGKEIELTHRMPTLKANIRAVKREQFRQQLKEKPSDDLESTSYFDPRVALAPALKPKKGFRFHEQGRFEKIAQRIRTKAQLEKLQMEIAQAAKKTGIQASTKLALIAPKKELGEGEIPNIEWWDSYILPFHINLSAHTNLDEVEMHGVTNLVEHPTQMAPPVDTDKPVTLGVYLTKKEQKKLRRQTRREGQKEVQEKVRLGLMPPPEPKVRISNLMRVLGTEAVQGPTKVEAHVRAQMAKRQKAHEEANAARKLTAEQRKEKKVKKLKEDLSHGVHIAVYRIRNLHNPAKKFKVEANANQLYLTGTVVLHKDVNIVVVEGGPKAQKKFKRLMLTRIKWDEPNSKRDEHHDDDESRRNNRCSLVWEGTSKERNYGEVKFKQCPTENMAREHFKKHGTEHYWDLALSQSVLETTDD

Target ORF information:

RefSeq Version	NM_205748.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio PRP3 pre-mRNA processing factor 3 homolog (yeast) (prpf3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205748.2

ORF Insert Sequence:

ATGTCACTGC CTAAACGTGA GGTTGATGAA CTGAGGCCAT GGGTGGAACG CACAGTAAAG 
AAGGTCCTTG GCTTCTCTGA ACCCACTGTG GTCACAGCAG CATTGCATTG TGTGGGAAAA 
GGCTTAGACA AAAGAAAAAC CATAGATCAG CTAAGACCAT TTCTTGATGA TTCGGCAAGC 
AGTTTCGTTG AGCGTCTGTT TGAAGCTCTC GAGGAAAGCA GAAACTCTCG TGGAAGTAAA 
GGAAGTGGAG AGAAAAACCG CAAAAGAGAA CTAAAAGAAG TGTTTGGAGA TGAGATGGAA 
GGGGTGAAGG ATGTAGTCAG TGTGGAATCA GCAGTAAAAA AGAAAAGGGT GCCCCGCTTT 
GAGGAGGTGG AAGAACCAGT TGTTTTACCT GGTCCCCCGA CTGAGAGTCC TGGCATGCTC 
ACTAAGATTC AGATAAAGCA AATGATGGAA GCAGCCACAC GACAGATTGA GGAGAGAAAG 
AAACAGCTAA GTTTTGCGGC TCCTACCCCC TCTTCGCAGT CAAGATTAAG TGCTTCTCAG 
ACAGACTCTA GTACTCCACG ATCTATGAAC CAAGCAGGGG GTGCAACCCA GTCCATCGCT 
CCTTCTCAGG CTGCTAATTT CATGAATGAT GCTATAGAAA AAGCACGCAA GGCAGCAGAG 
CTGCAGGCCA AGATCCAATC TACACTGTCC ATGAAACCAG GAATTCTGGC TACTGTGGGA 
AACCCTGGGC CACATAATAT GGTAGCACTG GCCAACCTAC ACGCCATGGG CATTGCACCA 
CCTAAAGTCG AACCTCGCGA GATCACAAAG CCAACACCTC TCATTTTGGA TGAGCTCGGA 
CGAACTGTTG ATGCCAGTGG GAAAGAAATT GAGCTCACAC ATCGTATGCC CACTCTCAAA 
GCAAACATCA GAGCTGTGAA GAGGGAACAG TTTCGACAAC AGTTAAAAGA AAAGCCTAGC 
GATGACCTAG AATCCACATC TTACTTTGAC CCACGTGTGG CCCTTGCCCC TGCACTAAAA 
CCCAAAAAAG GCTTTAGGTT CCATGAACAG GGGCGCTTTG AGAAGATAGC CCAGCGCATA 
CGCACCAAAG CTCAGCTGGA AAAATTACAA ATGGAGATTG CTCAGGCTGC CAAAAAGACT 
GGAATACAGG CCTCCACTAA ACTGGCTCTT ATTGCCCCAA AGAAGGAGCT GGGAGAGGGG 
GAGATTCCTA ATATAGAGTG GTGGGACTCC TACATACTGC CCTTCCATAT AAACCTATCT 
GCACATACAA ATTTAGATGA GGTGGAAATG CATGGAGTCA CCAACCTAGT TGAACATCCA 
ACTCAGATGG CTCCACCAGT GGATACCGAC AAGCCAGTTA CTCTTGGTGT GTATTTGACT 
AAGAAAGAAC AGAAGAAGTT GAGGAGACAA ACTCGCAGAG AAGGCCAGAA AGAGGTGCAG 
GAAAAAGTCA GATTAGGCCT TATGCCTCCA CCTGAACCCA AAGTGCGGAT CTCCAATCTG 
ATGAGGGTTT TGGGCACAGA AGCTGTGCAG GGCCCCACTA AAGTGGAGGC CCATGTTAGA 
GCACAAATGG CAAAGAGGCA GAAGGCACAT GAGGAGGCAA ATGCAGCACG CAAACTCACA 
GCAGAACAAA GGAAAGAGAA GAAGGTGAAA AAACTGAAGG AGGATTTGAG TCATGGAGTT 
CATATTGCTG TTTACAGAAT CCGAAATCTG CATAACCCAG CCAAGAAGTT TAAAGTGGAG 
GCCAATGCAA ACCAGCTGTA CCTCACTGGC ACTGTGGTAC TGCATAAAGA TGTCAACATC 
GTAGTTGTGG AAGGAGGACC CAAAGCACAG AAGAAGTTTA AGCGATTGAT GCTGACTCGG 
ATCAAATGGG ATGAGCCAAA TTCCAAAAGA GATGAGCATC ACGATGACGA TGAATCCAGG 
AGGAATAATA GATGCTCATT GGTCTGGGAG GGTACATCAA AAGAACGTAA CTATGGGGAG 
GTTAAGTTTA AGCAGTGTCC AACGGAGAAC ATGGCTCGGG AACATTTTAA GAAGCATGGC 
ACCGAGCACT ACTGGGACTT GGCTCTGAGC CAAAGTGTTC TGGAGACAAC TGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Knocking Down Snrnp200 Initiates Demorphogenesis of Rod Photoreceptors in Zebrafish.
Journal of ophthalmology2015816329(2015)
Liu Y,Chen X,Qin B,Zhao K,Zhao Q,Staley JP,Zhao C

PRPF4 mutations cause autosomal dominant retinitis pigmentosa.
Human molecular genetics23(11)2926-39(2014 Jun)
Chen X,Liu Y,Sheng X,Tam PO,Zhao K,Chen X,Rong W,Liu Y,Liu X,Pan X,Chen LJ,Zhao Q,Vollrath D,Pang CP,Zhao C

Sperm, but not oocyte, DNA methylome is inherited by zebrafish early embryos.
Cell153(4)773-84(2013 May)
Jiang L,Zhang J,Wang JJ,Wang L,Zhang L,Li G,Yang X,Ma X,Sun X,Cai J,Zhang J,Huang X,Yu M,Wang X,Liu F,Wu CI,He C,Zhang B,Ci W,Liu J

Decreased levels of the RNA splicing factor Prpf3 in mice and zebrafish do not cause photoreceptor degeneration.
Investigative ophthalmology & visual science49(9)3830-8(2008 Sep)
Graziotto JJ,Inglehearn CF,Pack MA,Pierce EA

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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prpf3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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