rnf20 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rnf20
Entrez Gene ID	402838
Full Name	ring finger protein 20, E3 ubiquitin protein ligase
General protein information	Preferred Names ring finger protein 20, E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase BRE1A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256175.1	NP_001243104.1	E3 ubiquitin-protein ligase BRE1A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RNF20	XP_005626937.1
Rattus norvegicus (Norway rat)	Rnf20	NP_001101399.2
Xenopus tropicalis (tropical clawed frog)	rnf20	XP_002941911.1
Mus musculus (house mouse)	Rnf20	NP_001156735.1
Gallus gallus (chicken)	RNF20	NP_001026605.1
Pan troglodytes (chimpanzee)	RNF20	XP_520159.2
Macaca mulatta (Rhesus monkey)	RNF20	XP_001110807.1
Bos taurus (cattle)	RNF20	NP_001075056.1
Danio rerio (zebrafish)	rnf20	NP_001243104.1
Homo sapiens (human)	RNF20	NP_062538.5

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rnf20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rnf20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31662	NM_001256175.1 Latest version!	Danio rerio ring finger protein 20, E3 ubiquitin protein ligase (rnf20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rnf20 ( NM_001256175.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001243104.1 Danio rerio ring finger protein 20, E3 ubiquitin protein ligase (rnf20), mRNA.

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CloneID	ODa31662
Clone ID Related Accession (Same CDS sequence)	NM_001256175.1
Accession Version	NM_001256175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase BRE1A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT956093.17. On Jan 12, 2012 this sequence version replaced XM_001923714.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01005529.1, GDQQ01004480.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCAGGTC AGAAGCGCCC CAGTGATGCC GGCAGCTCTG CCACCCTCGG TGCTCCTCCA 
GAGAAGAAGA AGGGAGGTGA AGATGGAGAG GGTATGAGCA CGGGATCAGG AGGAAGCACG 
GCTGTTGAAA CTGTCATCAA ACTGGGAGGA GGGGCCAACT TGGAAGAACA AGATATCAAA 
GCACTCCAGA TTAAAAACAG GAAGTTGGGA GAAGCTCTAG ACCAAAGACA GGTGATTGAG 
GATGAACTGA GGGAGCGAGT TGAGACGTTG GAGACTCGAC AGGCAACAGA TGATGCAAGT 
TTGTTGATTC TAAACCGATA CTGGAACCAG TTTGATGAAC ATGTACGATT ATTTATACGT 
CGCTGTGACC AATCGGGCAG TGAGCCTGTG GAAAGCCAAC CTCCTGAGGG GCGGAGCTTA 
AAACCTGGAA CACCAGAACC GGATGGAGAC TCAAACCAGG AGCGTGCAAA AGACAGAGGT 
CAGCAGGGGG AGGGAGTGAG CTCTTTTCTG GCTACACTGG CCAGCAGCAC CAGTGAGGAG 
ATCGACGCAG AGCTGCAGGA GCGAGTGGAG TCCAGCTGCA AACAGGCCAG TCGTGTGGTG 
GAAATTTATG AGAACCTGAA GAACATGGTG GATCAGCTAA AGAAGGATCT GGAGTCTGGA 
AAAGATGGCG GTCTCTGGGA CGTCGCCGTC CGCCTCAATG CCCTCCTGAC CGGTATGAAT 
GAGCGTCTCT GCCAGCTGAC CGATGGCCTC CAGCAGAAGC ACAGTCACAT GACCAGCGAG 
TCTCGTTCAC TGGGCCGGGC GGCTAATCGT GCTGATAACA GGATCAGTGA GCTGCAGGGG 
CTCATTGAGG AGCTGCAGTG GGACATGGAG AAGATCAGAC GCCGCGAGAA TCGGCTTAAC 
ACACACCTGG TGGAGGTGCT GGAAAGGGTG AACAGTAAAG GTTACAAGGT TTGTGGTGAA 
GCAAGCAGTG TATGTGGAAC CATCACTATC AACAAAAGAA AGTTTGAAGA GATGAACAGC 
GAATTAGAGG AGAACAGGGA GCTGGCGGAG AATCGGCTCA GTGAACTCCA GAAACTCCAG 
CAGGATCTTC AGACAGTTTA CCAGGAGAAT ACTAACATGA AGGTGGAGCT GTTGTCCAGA 
GCTGAGGAAG TGGCCAGAGA CAGTGCAGAG TATCGCTGTC TGCAGTCTCA ATTCTCTGTG 
CTCTACAATG AATCACTGGT GCTGAAATCT CAGCTGGATG AGACCAAAGC AAGACTCAAC 
ACGACCAGGA CTGCACGGCT GCGCCAGCTG GACCACATGG AGAATGATGA GGTCTCCTTG 
CAGCGGAAGG TCCGCACTGA AGTCATTCAG CTGGAAGACA CATTAGCACA AGTGCGCAAG 
GAGTACGAGA TGCTCAGGAT TGAATTTGAA CAGACTTTAG CTGCTAATGA ACAAGCAGGC 
CCCATAAACC GAGAGATGAG ACATTTGATC AGTACACTGC AAACTCATAA CCAGCAGATG 
AAGGGTGAGG TGGTCAAATA TAAACTCAGA CTGAGAGAGG CTCAGCAAGA GCTCAACCAG 
CTCCGGGCTG CCAAAGGCAA TGCTGCTGTT CAGTCTCAGT CCAGCACAGA GATGGATGTG 
AAGGAGGAGA CTGTCTCTCC CCTTACCCCT GCCCCGACCA CCGACGTTAC TGTGAAGACG 
GAGCCTGACA GCGGCTCTGC TACACCCAGC ACCACTGGTA TAACAGTCAA GACTGAACCA 
GGTACAGATG CAGAGACTGT GGTAAAAGAG GAGGAAAAGG AGAAGGAAAA AGACAAAGAA 
AAAGAAAAGG AAAAGGACAA AGAGAAGGAA CAGAGAGAAA GAGAGCGGGT GACGCGTGGC 
AGTACTGGAA GCTTAGGGGT GAAAGAAGAG AGAGACAAAG CTGGCACAAG CAGCAGCCAA 
TCAGAAGACA TCCCAGTTGA ACGCTGTGCT GTAATTGGAG GGCCTAAACG CAAGGAGATG 
GAGCAGCTGA AAATAGTGCG GGTGGAGCTG AAGAAAGCTC AAGAGTCCCA AAGGGAGATG 
AAGCTGTTGT TGGACATGTA CCGCTCAGCA CCAAAAGAGC AGAGGGATAA AGTACAGCTG 
ATGGCAGCTG AGAAGAAGAC AAAGTCAGAG GCCGAAGAGC TCCGTCAGAG GGTGCGAGAG 
CTGGAGGAGA GGGAAAGGAG AGAAGGAAAG AAGATGGCAG ATGAAGAAGC TCTCAGAAAG 
ATCCGCTCTG TGGAGGAGCA GATCGATATT CTTAATAAGA AACTCTCTCT TGCCAAGCAG 
GAGGAAGATG CTTTGCTGAG TGAAATGGAC GTGACGGGCC AGGCGTTTGA GGACATGCAG 
GAGCAGAACA TCCGTCTGAT GCAGCAGCTC AGAGAGAAAG ATGATGCCAA CTTCAAACTC 
ATGAGCGAGC GCATCAAATC CAACCAGATC CACAAACTCC TCAAAGAGGA GAAAGAGGAG 
CTCGCTGACC AGCTGCTCAC ACTTAAAACA CAGGTGGATG CTCAGCTACA GGTGGTTCGG 
AAGCTGGAAG AGAAAGAGCG CCTCCTGCAG GGCACCATCA GCGCAGCAGA GAGAGAGCTG 
GGGCTTCGCA CGCAGGCATT AGAGATGAAC AAACGCAAGG CGCAAGACTC TGTGCTGCTG 
TCGGAGGAGG TGCGGACTCA GCTGGAAGGG GTGCAGCAGA GGTTGCGTTC AGTCAGAGAA 
GAGGTGATTG AGAACAGCAT TTCACGAGAG AAGGAGTCTT TCAATGCCAG ACGTGCACAG 
GAGGACATAT CCAAACTCCG CAGGAAGATA GAGAAGGCCA AGAAACCGGC AGAGAACATT 
CGAAATGGAG ATGAGATCCT GAATGAAGAA ATAAACGAAT ACAAGGCCCG ATTGACTTGT 
CCATGCTGCA ATTCACGTGT AAAAGATGCA GTTCTGACAA AGTGCTTCCA CGTCTTCTGT 
TTCGAGTGCG TGAAGACACG TTACGACACC CGTCAGAGAA AGTGCCCAAA GTGCAATGCC 
GCATTTGGTG CCAACGACTT CCACCGCATT TACATCGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243104.1
CDS	150..3191
Translation	MSGQKRPSDAGSSATLGAPPEKKKGGEDGEGMSTGSGGSTAVETVIKLGGGANLEEQDIKALQIKNRKLGEALDQRQVIEDELRERVETLETRQATDDASLLILNRYWNQFDEHVRLFIRRCDQSGSEPVESQPPEGRSLKPGTPEPDGDSNQERAKDRGQQGEGVSSFLATLASSTSEEIDAELQERVESSCKQASRVVEIYENLKNMVDQLKKDLESGKDGGLWDVAVRLNALLTGMNERLCQLTDGLQQKHSHMTSESRSLGRAANRADNRISELQGLIEELQWDMEKIRRRENRLNTHLVEVLERVNSKGYKVCGEASSVCGTITINKRKFEEMNSELEENRELAENRLSELQKLQQDLQTVYQENTNMKVELLSRAEEVARDSAEYRCLQSQFSVLYNESLVLKSQLDETKARLNTTRTARLRQLDHMENDEVSLQRKVRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISTLQTHNQQMKGEVVKYKLRLREAQQELNQLRAAKGNAAVQSQSSTEMDVKEETVSPLTPAPTTDVTVKTEPDSGSATPSTTGITVKTEPGTDAETVVKEEEKEKEKDKEKEKEKDKEKEQRERERVTRGSTGSLGVKEERDKAGTSSSQSEDIPVERCAVIGGPKRKEMEQLKIVRVELKKAQESQREMKLLLDMYRSAPKEQRDKVQLMAAEKKTKSEAEELRQRVRELEERERREGKKMADEEALRKIRSVEEQIDILNKKLSLAKQEEDALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQLLTLKTQVDAQLQVVRKLEEKERLLQGTISAAERELGLRTQALEMNKRKAQDSVLLSEEVRTQLEGVQQRLRSVREEVIENSISREKESFNARRAQEDISKLRRKIEKAKKPAENIRNGDEILNEEINEYKARLTCPCCNSRVKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	NM_001256175.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ring finger protein 20, E3 ubiquitin protein ligase (rnf20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256175.1

ORF Insert Sequence:

ATGTCAGGTC AGAAGCGCCC CAGTGATGCC GGCAGCTCTG CCACCCTCGG TGCTCCTCCA 
GAGAAGAAGA AGGGAGGTGA AGATGGAGAG GGTATGAGCA CGGGATCAGG AGGAAGCACG 
GCTGTTGAAA CTGTCATCAA ACTGGGAGGA GGGGCCAACT TGGAAGAACA AGATATCAAA 
GCACTCCAGA TTAAAAACAG GAAGTTGGGA GAAGCTCTAG ACCAAAGACA GGTGATTGAG 
GATGAACTGA GGGAGCGAGT TGAGACGTTG GAGACTCGAC AGGCAACAGA TGATGCAAGT 
TTGTTGATTC TAAACCGATA CTGGAACCAG TTTGATGAAC ATGTACGATT ATTTATACGT 
CGCTGTGACC AATCGGGCAG TGAGCCTGTG GAAAGCCAAC CTCCTGAGGG GCGGAGCTTA 
AAACCTGGAA CACCAGAACC GGATGGAGAC TCAAACCAGG AGCGTGCAAA AGACAGAGGT 
CAGCAGGGGG AGGGAGTGAG CTCTTTTCTG GCTACACTGG CCAGCAGCAC CAGTGAGGAG 
ATCGACGCAG AGCTGCAGGA GCGAGTGGAG TCCAGCTGCA AACAGGCCAG TCGTGTGGTG 
GAAATTTATG AGAACCTGAA GAACATGGTG GATCAGCTAA AGAAGGATCT GGAGTCTGGA 
AAAGATGGCG GTCTCTGGGA CGTCGCCGTC CGCCTCAATG CCCTCCTGAC CGGTATGAAT 
GAGCGTCTCT GCCAGCTGAC CGATGGCCTC CAGCAGAAGC ACAGTCACAT GACCAGCGAG 
TCTCGTTCAC TGGGCCGGGC GGCTAATCGT GCTGATAACA GGATCAGTGA GCTGCAGGGG 
CTCATTGAGG AGCTGCAGTG GGACATGGAG AAGATCAGAC GCCGCGAGAA TCGGCTTAAC 
ACACACCTGG TGGAGGTGCT GGAAAGGGTG AACAGTAAAG GTTACAAGGT TTGTGGTGAA 
GCAAGCAGTG TATGTGGAAC CATCACTATC AACAAAAGAA AGTTTGAAGA GATGAACAGC 
GAATTAGAGG AGAACAGGGA GCTGGCGGAG AATCGGCTCA GTGAACTCCA GAAACTCCAG 
CAGGATCTTC AGACAGTTTA CCAGGAGAAT ACTAACATGA AGGTGGAGCT GTTGTCCAGA 
GCTGAGGAAG TGGCCAGAGA CAGTGCAGAG TATCGCTGTC TGCAGTCTCA ATTCTCTGTG 
CTCTACAATG AATCACTGGT GCTGAAATCT CAGCTGGATG AGACCAAAGC AAGACTCAAC 
ACGACCAGGA CTGCACGGCT GCGCCAGCTG GACCACATGG AGAATGATGA GGTCTCCTTG 
CAGCGGAAGG TCCGCACTGA AGTCATTCAG CTGGAAGACA CATTAGCACA AGTGCGCAAG 
GAGTACGAGA TGCTCAGGAT TGAATTTGAA CAGACTTTAG CTGCTAATGA ACAAGCAGGC 
CCCATAAACC GAGAGATGAG ACATTTGATC AGTACACTGC AAACTCATAA CCAGCAGATG 
AAGGGTGAGG TGGTCAAATA TAAACTCAGA CTGAGAGAGG CTCAGCAAGA GCTCAACCAG 
CTCCGGGCTG CCAAAGGCAA TGCTGCTGTT CAGTCTCAGT CCAGCACAGA GATGGATGTG 
AAGGAGGAGA CTGTCTCTCC CCTTACCCCT GCCCCGACCA CCGACGTTAC TGTGAAGACG 
GAGCCTGACA GCGGCTCTGC TACACCCAGC ACCACTGGTA TAACAGTCAA GACTGAACCA 
GGTACAGATG CAGAGACTGT GGTAAAAGAG GAGGAAAAGG AGAAGGAAAA AGACAAAGAA 
AAAGAAAAGG AAAAGGACAA AGAGAAGGAA CAGAGAGAAA GAGAGCGGGT GACGCGTGGC 
AGTACTGGAA GCTTAGGGGT GAAAGAAGAG AGAGACAAAG CTGGCACAAG CAGCAGCCAA 
TCAGAAGACA TCCCAGTTGA ACGCTGTGCT GTAATTGGAG GGCCTAAACG CAAGGAGATG 
GAGCAGCTGA AAATAGTGCG GGTGGAGCTG AAGAAAGCTC AAGAGTCCCA AAGGGAGATG 
AAGCTGTTGT TGGACATGTA CCGCTCAGCA CCAAAAGAGC AGAGGGATAA AGTACAGCTG 
ATGGCAGCTG AGAAGAAGAC AAAGTCAGAG GCCGAAGAGC TCCGTCAGAG GGTGCGAGAG 
CTGGAGGAGA GGGAAAGGAG AGAAGGAAAG AAGATGGCAG ATGAAGAAGC TCTCAGAAAG 
ATCCGCTCTG TGGAGGAGCA GATCGATATT CTTAATAAGA AACTCTCTCT TGCCAAGCAG 
GAGGAAGATG CTTTGCTGAG TGAAATGGAC GTGACGGGCC AGGCGTTTGA GGACATGCAG 
GAGCAGAACA TCCGTCTGAT GCAGCAGCTC AGAGAGAAAG ATGATGCCAA CTTCAAACTC 
ATGAGCGAGC GCATCAAATC CAACCAGATC CACAAACTCC TCAAAGAGGA GAAAGAGGAG 
CTCGCTGACC AGCTGCTCAC ACTTAAAACA CAGGTGGATG CTCAGCTACA GGTGGTTCGG 
AAGCTGGAAG AGAAAGAGCG CCTCCTGCAG GGCACCATCA GCGCAGCAGA GAGAGAGCTG 
GGGCTTCGCA CGCAGGCATT AGAGATGAAC AAACGCAAGG CGCAAGACTC TGTGCTGCTG 
TCGGAGGAGG TGCGGACTCA GCTGGAAGGG GTGCAGCAGA GGTTGCGTTC AGTCAGAGAA 
GAGGTGATTG AGAACAGCAT TTCACGAGAG AAGGAGTCTT TCAATGCCAG ACGTGCACAG 
GAGGACATAT CCAAACTCCG CAGGAAGATA GAGAAGGCCA AGAAACCGGC AGAGAACATT 
CGAAATGGAG ATGAGATCCT GAATGAAGAA ATAAACGAAT ACAAGGCCCG ATTGACTTGT 
CCATGCTGCA ATTCACGTGT AAAAGATGCA GTTCTGACAA AGTGCTTCCA CGTCTTCTGT 
TTCGAGTGCG TGAAGACACG TTACGACACC CGTCAGAGAA AGTGCCCAAA GTGCAATGCC 
GCATTTGGTG CCAACGACTT CCACCGCATT TACATCGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

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rnf20 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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