myo3a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	myo3a
Entrez Gene ID	402814
Full Name	myosin IIIA
General protein information	Preferred Names myosin IIIA Names myosin-IIIa
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001362412.1	NP_001349341.1	myosin-IIIa isoform 2
NM_205579.2	NP_991142.2	myosin-IIIa isoform 1
NM_205579.1	NP_991142.1	myosin-IIIa

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYO3A	XP_507703.2
Mus musculus (house mouse)	Myo3a	NP_680779.3
Macaca mulatta (Rhesus monkey)	MYO3A	XP_001101244.2
Xenopus tropicalis (tropical clawed frog)	myo3a	XP_002933189.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009730	XP_318814.4
Homo sapiens (human)	MYO3A	NP_059129.3
Rattus norvegicus (Norway rat)	Myo3a	XP_574090.4
Gallus gallus (chicken)	MYO3A	XP_418597.3
Canis lupus familiaris (dog)	MYO3A	XP_005617041.1
Drosophila melanogaster (fruit fly)	ninaC	NP_523503.2
Bos taurus (cattle)	MYO3A	XP_002692119.1
Danio rerio (zebrafish)	myo3a	NP_991142.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic control of cellular morphogenesis in Müller glia.
Charlton-Perkins M, Almeida AD, MacDonald RB, Harris WA
Glia67(7)1401-1411(2019 07)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Rbfox-regulated alternative splicing is critical for zebrafish cardiac and skeletal muscle functions.
Gallagher TL, Arribere JA, Geurts PA, Exner CR, McDonald KL, Dill KK, Marr HL, Adkar SS, Garnett AT, Amacher SL, Conboy JG
Developmental biology359(2)251-61(2011 Nov)

Identification and localization of myosin superfamily members in fish retina and retinal pigmented epithelium.
Lin-Jones J, Sohlberg L, Dosé A, Breckler J, Hillman DW, Burnside B
The Journal of comparative neurology513(2)209-23(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following myo3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myo3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa05145	NM_205579.1 Latest version!	Danio rerio myosin IIIA (myo3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa159816	NM_205579.2 Latest version!	Danio rerio myosin IIIA (myo3a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa159841	NM_001362412.1 Latest version!	Danio rerio myosin IIIA (myo3a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myo3a ( NM_205579.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991142.1 Danio rerio myosin IIIA (myo3a), mRNA.

Price & Availability↑

CloneID	ODa05145
Clone ID Related Accession (Same CDS sequence)	NM_205579.1
Accession Version	NM_205579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1501862400000
Organism	Danio rerio(zebrafish)
Product	myosin-IIIa
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF384863.1. ##Evidence-Data-START## Transcript exon combination :: AF384863.1 [ECO:0000332] ##Evidence-Data-END##

ATGTTTCCTC GCTCAGGGAA ATCCATCATC TTTGACAGCT TTCCAGATCC GTCTGACACC 
TGGGAGATCA TTGAGACTAT TGGCAAAGGG ACTTATGGGA AAGTTTACAA AGTGCATAAT 
AAGGTCGATG GCAGCAAAGC CGCAGTCAAA ATACTGGATC CGATCCATGA CATCGATGAA 
GAAATTGAAG CTGAGTATAA CATCCTGAAA GCTCTTTCAG ACCATCCCAA TGTTGTTAAA 
TTCTTTGGGA TGTTCTACAA GAAGGATGTC AAGACTGGAG ACCAGCTATG GCTAGTACTA 
GAGCTGTGTA ACGGAGGGTC TGTCACAGAT CTGGCCAAAG GTATGCTAAA AAGAGGAGAT 
CGCATGGAGG AACCCATCAT AGCCTTTATT CTACATGAAG CGCTCATGGG TCTTCAACAT 
TTGCACGTCA ATAAGACCAT CCATCGAGAC GTCAAGGGCA ACAACATCCT GCTCACCACG 
GGCGGAGGAA TCAAGCTGGT TGACTTTGGG GTTTCAGCCC AGCTCACAAA CACCCGACTA 
AGGAGGAACA CTTCGGTGGG AACGCCCTTC TGGATGGCTC CTGAGGTCAT CGCGTGTGAG 
CAGCAGCTGG ACTCCACATA TGATGAACGC TGTGATGTTT GGTCATTGGG AATCACAGCC 
ATAGAGCTAG GTGATGGAGA CCCGCCTCTC GCTGAACTCC ATCCAATGAG AGCTCTCTTC 
AAAATACCAA GAAATCCACC ACCCACTCTA CACCAGCCAG AGCTCTGGTC AAACGACTTT 
AACGATTTCA TACGCAAGTG TCTGATAAAG GATTTTGAGC TCAGGCCCAA TGTGCTTGAC 
CTTCTTCAGC ATGTCTTCAT CAAACAGATT GTGGGCCGAG AGAAGACGCT GCAGAAGCAG 
CTGATGGAGC TAATCGACCT CAACCAGCAA ATGGGCATCA TTGAGAAAAC AAGCCTTCTC 
GGAAAATCTG ACAGGGACAG TGACTCCAGG CATGAGCGTA TCCACACCAA GAAAAACAGC 
TACATGAAGT CTCAAAGTCA AACGTGTGAC GACGCTGATG ATCTGGCAAC CCTAGAGGTT 
CTAGATGAAA ACACAATCAC CGAACAGCTA CAGAGACGTT ACACAAAGGA GCAGATCTAC 
ACCTACGTCG GAGACATCCT TATAGCGGTC AATCCATTCC ACAAAACCGA GTTATACACA 
CCTGAGCATA CTAAGAGTTA TGTTGGCGCC AAACGAACAT CAAACCCCCC ACATATATAT 
GCAGTGGCAG ACATTGCGTA TCAGTCCATG GTGTCCTATA ATGCAGATCA GTGTATTGTC 
ATCAGTGGAG AAAGCGGCGC TGGGAAAACA GAGAGTGCTC ACCTGCTAGT CCAACAGCTG 
ACCGTCCTTG GAAAGGCCAA CAATCGGACC CTCCAGGAGA AGATTTTGCT GGTTAACAGT 
CTAGTCGAGG CATTCGGAAA TGCCTGCACT GTGATCAATG ATAACTCCAG TCGCTTTGGC 
AAATATCTGG AGATGAAGTT CACCTGTGGT GGGACAGTAG TGGGAGCTCA GATCTCTGAT 
TATCTGCTGG AGAAGTCTCG AGTCGTCCAC CATGCAGTAG GGGAACGTAA CTTCCACATC 
TTCTATTATA TCTACGCCGG ACTGGCAGAG AGGAAGAAAC TCGGCCATTA CAAGCTGTCA 
GACAGCAAAA CTCCAAGGTA TCTCCACAAT GAGAATGTTA AATTAGTGCC TGACATTGTG 
GGCAATGCCT TCTATAAGGA GCAGTTTGAG ACTGTGGAGC AGTGCTTCAA AGTCATCGGC 
TTCACCCTAG AGGAGCTCGG GAGTGTTTAC GGCATCTTAG CTGGGATCCT GAATACAGGA 
GACGTCGAGT TCACGTCAGT GGCTTCAGAA CATCAAACGG ACAAAAGCAA CATTTCCAAC 
ATGGTAGTAC TAGAGAGTGC TGCATCGCTG CTGTGTATCC GTGCAGATGA GCTACAGGAG 
GCTCTGACAT CTCATTGTGT GGTCACTCGG GGTGAAACCA TCGTCAGGTC AAACACAGTG 
GAGAAGGCCA CAGAGGCGCG GGACGCCATG GGTAAAGCCC TATATGGACG CCTGTTCAGC 
TGGATTGTCA ACCGCATCAA CTCTCTGCTC AAACCAGACA ACCAATCAGG GGAGGACGAC 
AAGGGTCTGA ACATCGGCAT CCTGGACATC TTCGGCTTTG AAAACTTTAA GAGGAACTCT 
TTCGAACAGC TCTGCATCAA CATCGCCAAT GAGCAGATCC AGTTCTACTT CAACCAGCAC 
ATCTTCGCCT GGGAACAGGA TGAGTATTTG AACGAAGATG TAGATGCTCG AGTAATTGAG 
TATGAAGACA ACCGGCCACT GCTGGACCTG TTCCTTCAGA AGCCCATGGG CATGTTATCG 
CTGCTGGACG AGGAGAGTCG CTTTCCTCAG GCAACTGACC AGACCCTCGT CGAGAAATTT 
GAGGATAACT TAAAGTCCAA AAACTTCTGG AGACCAAAGA GGGTTGACCT GGGCTTTGGA 
ATCCATCACT ATGCAGGCAA GGTGATCTAT AATGCCGGCG GCTTCCTGGC TAAGAACAGA 
GACGCTTTGC CTGCTGATAT AGTTCTTCTG CTGAGGTCAT CAGAGAATGA ACTGACCCGT 
AAACTGGTTA CGCATCCGCT CACTAAAACA GGTAATTTGG CGCACACTAA GGGCAAAGCT 
GTGGGCTCTG CTCGGATGGG CACACAACAA TCACAGCGCT TTGCCAAGTT GGATTGCCTT 
TTGTTGTTCA GTTCTGTATC ATTCAGTGAG TCTTCCTCTC GGAAATTAAC TCCACCCATT 
GACAAATCAA TGCTTGCCTT GCCTGTTGAC TCTGTACAAA CGACGGAGAG CACTGGAGAC 
ACCACACATC ACCCCAGGGA GACCACCAAC ATGAAGACTC AAACTGTAGC GTCGTACTTC 
AGATATTCTC TAATGGACCT GCTATCAAAG ATGGTGGCAG GCCAGCCTCA TTTTGTTCGA 
TGCATAAAAC CCAACAATGA TCGTCAAGCT CACAAGTTTG ACAGGGAAAA GGTTCTGATC 
CAACTACGCT ACACTGGTAT TCTAGAGACG GCCAAGATCC GCAGACAAGG CTATTCTCAT 
CGCATTCTCT TCAACAACTT CATCGAAAGG TACTCCATAT TAGCATTTAG AGCTAATGAA 
GAGCCAGCAA TCAGTCCTGA GACGTGTGCA GTTATTTTAG AGAAGGCCAA ACTGGAGAAC 
TGGATTTTGG GCAAGACAAA GGTCTTTCTG AAGTATTATC ATGTGGAACA GCTGAATCTG 
ATGGTCAGGC AGACCACTGA CCGGATTGTT CTAGTTCAGG CATATGTACG GGGCTGGATG 
GCATTCAAGC GATACTGTTT GCTGAAGGAA AAGAGAGAAC AAAGCGCTGT TGTGTTACAA 
TCAGCATACA GAGGACACAA GGCTCGGAAA AGTATAGCTG ATGACAGAAG CAAAGCAAAG 
TCTGAAATAG TTTTCACCCA ACTTCAAGCT TGGTGCAGAG GATATTTGGC AAGGCAGAAT 
TACAAGGAGC TACTAGATGA GAAGAACAAA GCTGCTGCAA AGATCCAGGC CCATTACAGG 
GGACAAAAAG AGAGGAAGAG TTTCCAGAGG AAAAAGGAAG CCATGCAGAA GGAAAACACA 
GAGAAGACAG AAAGAGTGAC AGAGGTCCCT TTAGAAGCAG ACGATCCTGT TTCAGAGGAA 
CCGCTACAGG AAGGAGGTGA AGTGGAAATG GATGATGCTG ATGAAAAGGC AGCTGTGGTC 
CTCCAAAGCA ACTACCGAGG ATACAGAGAA AGGAAGAAAT TCAAGGAGCG TCAGGAACGG 
AATAAGACCT TGAGCGGAGA TGAGCTGGAT GCTTCCTCAT CCACCCCGGA AGAAATGACT 
TCTGGAGAAG ATCTGGAAGC TACACGAGAG GACACGCAGG AGAATGTTGA GAAAGAGGGG 
AATGAGGTTG AGGAAGGTGG AGGAGATGAA GACCCTGAGC AGGAAGCCAA GGCAGCCACT 
GTTCTCCAGA GCAACTTCAG AGGTCATAAG GAACGTAAAA GACTCCAAGA GGAAGGAAAG 
ATACCAGTTA GAGGAAAGAA GGAGATTAAG AGTCCTACAG ATGAGGAGGA GCTTCCTATT 
CCAGATCCAC CGCCCCCTGA GGAACTGATG GAGTTTTCAG AAGAAATGGA ATTTACTGAA 
GAAAATCCAC CAGGAGAAGA AGACGAGGCC AAAGCTGCTG TGGTCCTTCA GAGCAACTTC 
CGTGGCCACA AAGAAAGGAA AAGACTCCAA GAGGAAGGGA AAATCCCAAA GAAAAAGACT 
AAAGAGGAAA TGGATGATCC AGGAGCTTCT GAACCAGACA TTGCCATTGA GGAGCTTCCA 
CCGCCTCCTT TAGAACTTGA ACATGATCAA GAGCTAGACT CTAGTCCATC AGGGGGAGAT 
GAGGAACACG CTGCAACTGT TTTACAAAGC AATTTCAGAG GACACCGGGA GAGAAAGAGA 
CTGAGAGCTG AGAAAGAAGA GGCAACAGGA GGAGGGCAAA GCACTGGAGA TGTGGAGGAG 
CAGGAGAACA ATGACGTTCT AGACATTACA GATGTTCAGC TGGAACGGAG GGAAGAAGAG 
GAGGAAGGAC AATACGATGA GGAGCAGGCA GCCATTAAAA TCCAGAGCAA TTTTAGAGGC 
TATAAAGACC GAAAGAACCT GAAAGCTACA TCACAAAAAC AAGATGAGGA AGTGGAGTCA 
CTCTTCAAAC AGGTATCCAA GGCCTCTGAA GACTACCTTT CCCTTCAGAA GAAGTTAAAT 
GAAATCATCC TTGCCCATCA GCTTAACCCC AGCAAAAATG GCATGTTTGT GAAAGGACAA 
CCCATGAATG GCTACATGGG CTACTACCAG CCACCGGCTG ACACAAAGCA GTCTCGAACA 
CCTCGCCGCG CACAACAACC CAAGACCCTG AACACGCCGG AGGACTCCAC ATACTACACA 
CTTATTCATA GGTCTGTCCA GGACGATAAA AGGAGGCCGC GGAAACAAAG CCCAAAAAAG 
CTGGACAACG ATGACCAATA CTACCAGGGT TTGTCCAGCA GTAAATCCAC CAGCCTTCCC 
AATGAACGAC GCCGGTCGAG CAAGCGGAGG GCTTCTGATG AAAGACGCTC CTCTGAAAGA 
CCACTCTCTG ATGTCCCCAA ATCTGAAATC CCCAAAGACC CATTGGAGAA GAGGCACTCG 
TTCTCTAAAA TGCCCTCTAC AGAGAGTCAA AGCGAGGACA ACCCCTACGA CTACAGGAAG 
ATACTGAGAA AGACCTCCCA GAGACGAAGG CTAATAACAC AGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991142.1
CDS	113..5440
Translation	MFPRSGKSIIFDSFPDPSDTWEIIETIGKGTYGKVYKVHNKVDGSKAAVKILDPIHDIDEEIEAEYNILKALSDHPNVVKFFGMFYKKDVKTGDQLWLVLELCNGGSVTDLAKGMLKRGDRMEEPIIAFILHEALMGLQHLHVNKTIHRDVKGNNILLTTGGGIKLVDFGVSAQLTNTRLRRNTSVGTPFWMAPEVIACEQQLDSTYDERCDVWSLGITAIELGDGDPPLAELHPMRALFKIPRNPPPTLHQPELWSNDFNDFIRKCLIKDFELRPNVLDLLQHVFIKQIVGREKTLQKQLMELIDLNQQMGIIEKTSLLGKSDRDSDSRHERIHTKKNSYMKSQSQTCDDADDLATLEVLDENTITEQLQRRYTKEQIYTYVGDILIAVNPFHKTELYTPEHTKSYVGAKRTSNPPHIYAVADIAYQSMVSYNADQCIVISGESGAGKTESAHLLVQQLTVLGKANNRTLQEKILLVNSLVEAFGNACTVINDNSSRFGKYLEMKFTCGGTVVGAQISDYLLEKSRVVHHAVGERNFHIFYYIYAGLAERKKLGHYKLSDSKTPRYLHNENVKLVPDIVGNAFYKEQFETVEQCFKVIGFTLEELGSVYGILAGILNTGDVEFTSVASEHQTDKSNISNMVVLESAASLLCIRADELQEALTSHCVVTRGETIVRSNTVEKATEARDAMGKALYGRLFSWIVNRINSLLKPDNQSGEDDKGLNIGILDIFGFENFKRNSFEQLCINIANEQIQFYFNQHIFAWEQDEYLNEDVDARVIEYEDNRPLLDLFLQKPMGMLSLLDEESRFPQATDQTLVEKFEDNLKSKNFWRPKRVDLGFGIHHYAGKVIYNAGGFLAKNRDALPADIVLLLRSSENELTRKLVTHPLTKTGNLAHTKGKAVGSARMGTQQSQRFAKLDCLLLFSSVSFSESSSRKLTPPIDKSMLALPVDSVQTTESTGDTTHHPRETTNMKTQTVASYFRYSLMDLLSKMVAGQPHFVRCIKPNNDRQAHKFDREKVLIQLRYTGILETAKIRRQGYSHRILFNNFIERYSILAFRANEEPAISPETCAVILEKAKLENWILGKTKVFLKYYHVEQLNLMVRQTTDRIVLVQAYVRGWMAFKRYCLLKEKREQSAVVLQSAYRGHKARKSIADDRSKAKSEIVFTQLQAWCRGYLARQNYKELLDEKNKAAAKIQAHYRGQKERKSFQRKKEAMQKENTEKTERVTEVPLEADDPVSEEPLQEGGEVEMDDADEKAAVVLQSNYRGYRERKKFKERQERNKTLSGDELDASSSTPEEMTSGEDLEATREDTQENVEKEGNEVEEGGGDEDPEQEAKAATVLQSNFRGHKERKRLQEEGKIPVRGKKEIKSPTDEEELPIPDPPPPEELMEFSEEMEFTEENPPGEEDEAKAAVVLQSNFRGHKERKRLQEEGKIPKKKTKEEMDDPGASEPDIAIEELPPPPLELEHDQELDSSPSGGDEEHAATVLQSNFRGHRERKRLRAEKEEATGGGQSTGDVEEQENNDVLDITDVQLERREEEEEGQYDEEQAAIKIQSNFRGYKDRKNLKATSQKQDEEVESLFKQVSKASEDYLSLQKKLNEIILAHQLNPSKNGMFVKGQPMNGYMGYYQPPADTKQSRTPRRAQQPKTLNTPEDSTYYTLIHRSVQDDKRRPRKQSPKKLDNDDQYYQGLSSSKSTSLPNERRRSSKRRASDERRSSERPLSDVPKSEIPKDPLEKRHSFSKMPSTESQSEDNPYDYRKILRKTSQRRRLITQP

Target ORF information:

RefSeq Version	NM_205579.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin IIIA (myo3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205579.1

ORF Insert Sequence:

ATGTTTCCTC GCTCAGGGAA ATCCATCATC TTTGACAGCT TTCCAGATCC GTCTGACACC 
TGGGAGATCA TTGAGACTAT TGGCAAAGGG ACTTATGGGA AAGTTTACAA AGTGCATAAT 
AAGGTCGATG GCAGCAAAGC CGCAGTCAAA ATACTGGATC CGATCCATGA CATCGATGAA 
GAAATTGAAG CTGAGTATAA CATCCTGAAA GCTCTTTCAG ACCATCCCAA TGTTGTTAAA 
TTCTTTGGGA TGTTCTACAA GAAGGATGTC AAGACTGGAG ACCAGCTATG GCTAGTACTA 
GAGCTGTGTA ACGGAGGGTC TGTCACAGAT CTGGCCAAAG GTATGCTAAA AAGAGGAGAT 
CGCATGGAGG AACCCATCAT AGCCTTTATT CTACATGAAG CGCTCATGGG TCTTCAACAT 
TTGCACGTCA ATAAGACCAT CCATCGAGAC GTCAAGGGCA ACAACATCCT GCTCACCACG 
GGCGGAGGAA TCAAGCTGGT TGACTTTGGG GTTTCAGCCC AGCTCACAAA CACCCGACTA 
AGGAGGAACA CTTCGGTGGG AACGCCCTTC TGGATGGCTC CTGAGGTCAT CGCGTGTGAG 
CAGCAGCTGG ACTCCACATA TGATGAACGC TGTGATGTTT GGTCATTGGG AATCACAGCC 
ATAGAGCTAG GTGATGGAGA CCCGCCTCTC GCTGAACTCC ATCCAATGAG AGCTCTCTTC 
AAAATACCAA GAAATCCACC ACCCACTCTA CACCAGCCAG AGCTCTGGTC AAACGACTTT 
AACGATTTCA TACGCAAGTG TCTGATAAAG GATTTTGAGC TCAGGCCCAA TGTGCTTGAC 
CTTCTTCAGC ATGTCTTCAT CAAACAGATT GTGGGCCGAG AGAAGACGCT GCAGAAGCAG 
CTGATGGAGC TAATCGACCT CAACCAGCAA ATGGGCATCA TTGAGAAAAC AAGCCTTCTC 
GGAAAATCTG ACAGGGACAG TGACTCCAGG CATGAGCGTA TCCACACCAA GAAAAACAGC 
TACATGAAGT CTCAAAGTCA AACGTGTGAC GACGCTGATG ATCTGGCAAC CCTAGAGGTT 
CTAGATGAAA ACACAATCAC CGAACAGCTA CAGAGACGTT ACACAAAGGA GCAGATCTAC 
ACCTACGTCG GAGACATCCT TATAGCGGTC AATCCATTCC ACAAAACCGA GTTATACACA 
CCTGAGCATA CTAAGAGTTA TGTTGGCGCC AAACGAACAT CAAACCCCCC ACATATATAT 
GCAGTGGCAG ACATTGCGTA TCAGTCCATG GTGTCCTATA ATGCAGATCA GTGTATTGTC 
ATCAGTGGAG AAAGCGGCGC TGGGAAAACA GAGAGTGCTC ACCTGCTAGT CCAACAGCTG 
ACCGTCCTTG GAAAGGCCAA CAATCGGACC CTCCAGGAGA AGATTTTGCT GGTTAACAGT 
CTAGTCGAGG CATTCGGAAA TGCCTGCACT GTGATCAATG ATAACTCCAG TCGCTTTGGC 
AAATATCTGG AGATGAAGTT CACCTGTGGT GGGACAGTAG TGGGAGCTCA GATCTCTGAT 
TATCTGCTGG AGAAGTCTCG AGTCGTCCAC CATGCAGTAG GGGAACGTAA CTTCCACATC 
TTCTATTATA TCTACGCCGG ACTGGCAGAG AGGAAGAAAC TCGGCCATTA CAAGCTGTCA 
GACAGCAAAA CTCCAAGGTA TCTCCACAAT GAGAATGTTA AATTAGTGCC TGACATTGTG 
GGCAATGCCT TCTATAAGGA GCAGTTTGAG ACTGTGGAGC AGTGCTTCAA AGTCATCGGC 
TTCACCCTAG AGGAGCTCGG GAGTGTTTAC GGCATCTTAG CTGGGATCCT GAATACAGGA 
GACGTCGAGT TCACGTCAGT GGCTTCAGAA CATCAAACGG ACAAAAGCAA CATTTCCAAC 
ATGGTAGTAC TAGAGAGTGC TGCATCGCTG CTGTGTATCC GTGCAGATGA GCTACAGGAG 
GCTCTGACAT CTCATTGTGT GGTCACTCGG GGTGAAACCA TCGTCAGGTC AAACACAGTG 
GAGAAGGCCA CAGAGGCGCG GGACGCCATG GGTAAAGCCC TATATGGACG CCTGTTCAGC 
TGGATTGTCA ACCGCATCAA CTCTCTGCTC AAACCAGACA ACCAATCAGG GGAGGACGAC 
AAGGGTCTGA ACATCGGCAT CCTGGACATC TTCGGCTTTG AAAACTTTAA GAGGAACTCT 
TTCGAACAGC TCTGCATCAA CATCGCCAAT GAGCAGATCC AGTTCTACTT CAACCAGCAC 
ATCTTCGCCT GGGAACAGGA TGAGTATTTG AACGAAGATG TAGATGCTCG AGTAATTGAG 
TATGAAGACA ACCGGCCACT GCTGGACCTG TTCCTTCAGA AGCCCATGGG CATGTTATCG 
CTGCTGGACG AGGAGAGTCG CTTTCCTCAG GCAACTGACC AGACCCTCGT CGAGAAATTT 
GAGGATAACT TAAAGTCCAA AAACTTCTGG AGACCAAAGA GGGTTGACCT GGGCTTTGGA 
ATCCATCACT ATGCAGGCAA GGTGATCTAT AATGCCGGCG GCTTCCTGGC TAAGAACAGA 
GACGCTTTGC CTGCTGATAT AGTTCTTCTG CTGAGGTCAT CAGAGAATGA ACTGACCCGT 
AAACTGGTTA CGCATCCGCT CACTAAAACA GGTAATTTGG CGCACACTAA GGGCAAAGCT 
GTGGGCTCTG CTCGGATGGG CACACAACAA TCACAGCGCT TTGCCAAGTT GGATTGCCTT 
TTGTTGTTCA GTTCTGTATC ATTCAGTGAG TCTTCCTCTC GGAAATTAAC TCCACCCATT 
GACAAATCAA TGCTTGCCTT GCCTGTTGAC TCTGTACAAA CGACGGAGAG CACTGGAGAC 
ACCACACATC ACCCCAGGGA GACCACCAAC ATGAAGACTC AAACTGTAGC GTCGTACTTC 
AGATATTCTC TAATGGACCT GCTATCAAAG ATGGTGGCAG GCCAGCCTCA TTTTGTTCGA 
TGCATAAAAC CCAACAATGA TCGTCAAGCT CACAAGTTTG ACAGGGAAAA GGTTCTGATC 
CAACTACGCT ACACTGGTAT TCTAGAGACG GCCAAGATCC GCAGACAAGG CTATTCTCAT 
CGCATTCTCT TCAACAACTT CATCGAAAGG TACTCCATAT TAGCATTTAG AGCTAATGAA 
GAGCCAGCAA TCAGTCCTGA GACGTGTGCA GTTATTTTAG AGAAGGCCAA ACTGGAGAAC 
TGGATTTTGG GCAAGACAAA GGTCTTTCTG AAGTATTATC ATGTGGAACA GCTGAATCTG 
ATGGTCAGGC AGACCACTGA CCGGATTGTT CTAGTTCAGG CATATGTACG GGGCTGGATG 
GCATTCAAGC GATACTGTTT GCTGAAGGAA AAGAGAGAAC AAAGCGCTGT TGTGTTACAA 
TCAGCATACA GAGGACACAA GGCTCGGAAA AGTATAGCTG ATGACAGAAG CAAAGCAAAG 
TCTGAAATAG TTTTCACCCA ACTTCAAGCT TGGTGCAGAG GATATTTGGC AAGGCAGAAT 
TACAAGGAGC TACTAGATGA GAAGAACAAA GCTGCTGCAA AGATCCAGGC CCATTACAGG 
GGACAAAAAG AGAGGAAGAG TTTCCAGAGG AAAAAGGAAG CCATGCAGAA GGAAAACACA 
GAGAAGACAG AAAGAGTGAC AGAGGTCCCT TTAGAAGCAG ACGATCCTGT TTCAGAGGAA 
CCGCTACAGG AAGGAGGTGA AGTGGAAATG GATGATGCTG ATGAAAAGGC AGCTGTGGTC 
CTCCAAAGCA ACTACCGAGG ATACAGAGAA AGGAAGAAAT TCAAGGAGCG TCAGGAACGG 
AATAAGACCT TGAGCGGAGA TGAGCTGGAT GCTTCCTCAT CCACCCCGGA AGAAATGACT 
TCTGGAGAAG ATCTGGAAGC TACACGAGAG GACACGCAGG AGAATGTTGA GAAAGAGGGG 
AATGAGGTTG AGGAAGGTGG AGGAGATGAA GACCCTGAGC AGGAAGCCAA GGCAGCCACT 
GTTCTCCAGA GCAACTTCAG AGGTCATAAG GAACGTAAAA GACTCCAAGA GGAAGGAAAG 
ATACCAGTTA GAGGAAAGAA GGAGATTAAG AGTCCTACAG ATGAGGAGGA GCTTCCTATT 
CCAGATCCAC CGCCCCCTGA GGAACTGATG GAGTTTTCAG AAGAAATGGA ATTTACTGAA 
GAAAATCCAC CAGGAGAAGA AGACGAGGCC AAAGCTGCTG TGGTCCTTCA GAGCAACTTC 
CGTGGCCACA AAGAAAGGAA AAGACTCCAA GAGGAAGGGA AAATCCCAAA GAAAAAGACT 
AAAGAGGAAA TGGATGATCC AGGAGCTTCT GAACCAGACA TTGCCATTGA GGAGCTTCCA 
CCGCCTCCTT TAGAACTTGA ACATGATCAA GAGCTAGACT CTAGTCCATC AGGGGGAGAT 
GAGGAACACG CTGCAACTGT TTTACAAAGC AATTTCAGAG GACACCGGGA GAGAAAGAGA 
CTGAGAGCTG AGAAAGAAGA GGCAACAGGA GGAGGGCAAA GCACTGGAGA TGTGGAGGAG 
CAGGAGAACA ATGACGTTCT AGACATTACA GATGTTCAGC TGGAACGGAG GGAAGAAGAG 
GAGGAAGGAC AATACGATGA GGAGCAGGCA GCCATTAAAA TCCAGAGCAA TTTTAGAGGC 
TATAAAGACC GAAAGAACCT GAAAGCTACA TCACAAAAAC AAGATGAGGA AGTGGAGTCA 
CTCTTCAAAC AGGTATCCAA GGCCTCTGAA GACTACCTTT CCCTTCAGAA GAAGTTAAAT 
GAAATCATCC TTGCCCATCA GCTTAACCCC AGCAAAAATG GCATGTTTGT GAAAGGACAA 
CCCATGAATG GCTACATGGG CTACTACCAG CCACCGGCTG ACACAAAGCA GTCTCGAACA 
CCTCGCCGCG CACAACAACC CAAGACCCTG AACACGCCGG AGGACTCCAC ATACTACACA 
CTTATTCATA GGTCTGTCCA GGACGATAAA AGGAGGCCGC GGAAACAAAG CCCAAAAAAG 
CTGGACAACG ATGACCAATA CTACCAGGGT TTGTCCAGCA GTAAATCCAC CAGCCTTCCC 
AATGAACGAC GCCGGTCGAG CAAGCGGAGG GCTTCTGATG AAAGACGCTC CTCTGAAAGA 
CCACTCTCTG ATGTCCCCAA ATCTGAAATC CCCAAAGACC CATTGGAGAA GAGGCACTCG 
TTCTCTAAAA TGCCCTCTAC AGAGAGTCAA AGCGAGGACA ACCCCTACGA CTACAGGAAG 
ATACTGAGAA AGACCTCCCA GAGACGAAGG CTAATAACAC AGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo3a ( NM_205579.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991142.2 Danio rerio myosin IIIA (myo3a), transcript variant 1, mRNA.

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CloneID	ODa159816
Clone ID Related Accession (Same CDS sequence)	NM_205579.2
Accession Version	NM_205579.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570809600000
Organism	Danio rerio(zebrafish)
Product	myosin-IIIa isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX530066.21. On Apr 14, 2018 this sequence version replaced NM_205579.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF384863.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTTCCTC GCTCAGGGAA ATCCATCATC TTTGACAGCT TCCCAGATCC GTCTGACACC 
TGGGAGATCA TTGAGACTAT TGGCAAAGGG ACTTATGGGA AAGTTTACAA AGTGCATAAT 
AAGGTCGATG GCAGCAAAGC CGCAGTCAAA ATACTGGATC CAATCCATGA CATCGATGAA 
GAAATTGAAG CTGAGTATAA CATCCTGAAA GCTCTTTCAG ACCATCCCAA TGTTGTTAAA 
TTCTTTGGGA TGTTCTACAA GAAGGATGTC AAGACTGGAG ACCAGCTATG GCTAGTACTA 
GAGCTGTGTA ACGGAGGGTC TGTCACAGAT CTGGCCAAAG GTATGCTAAA AAGAGGAGAT 
CGCATGGAGG AACCCATCAT AGCCTTTATT CTACATGAAG CGCTCATGGG TCTTCAACAT 
TTGCACGTCA ATAAGACCAT CCATCGAGAC GTCAAGGGCA ACAACATCCT GCTCACCACG 
GACGGAGGAA TCAAGCTGGT TGACTTTGGG GTTTCAGCCC AGCTCACAAA CACCCGACTA 
AGGAGGAACA CTTCGGTGGG AACGCCCTTC TGGATGGCTC CTGAGGTCAT CGCGTGTGAG 
CAGCAGCTGG ACTCCACATA TGATGAACGC TGTGATGTTT GGTCATTGGG AATCACAGCC 
ATAGAGCTAG GTGATGGAGA CCCGCCTCTC GCTGAACTCC ATCCAATGAG AGCTCTCTTC 
AAAATACCAA GAAATCCACC ACCCACTCTA CACCAGCCAG AGCTCTGGTC AAACGACTTT 
AACGATTTCA TACGCAAGTG TCTGATAAAG GATTTTGAGC TCAGGCCCAA TGTGCTTGAC 
CTTCTTCAGC ATGTCTTCAT CAAACAGATT GTGGGCCGAG AGAAGACGCT GCAGAAGCAG 
CTGATGGAGC TAATCGACCT CAACCAGCAA ATGGGCATCA TTGAGAAAAC AAGCCTTCTC 
GGAAAATCTG ACAGGGACAG TGACTCCAGG CATGAGCGTA TCCACACCAA GAAAAACAGC 
TACATGAAGT CTCAAAGTCA AACGTGTGAC GACGCTGATG ATCTGGCAAC CCTAGAGGTT 
CTAGATGAAA ACACAATCAC CGAACAGCTA CAGAGACGTT ACACAAAGGA GCAGATCTAC 
ACCTACGTCG GAGACATCCT TATAGCGGTC AATCCATTTC ACAAAACCGA GTTATACACA 
CCTGAGCATA CTAAGAGTTA TGTTGGCGCC AAACGAACAT CAAACCCCCC ACATATATAT 
GCAGTGGCAG ACATTGCGTA TCAGTCCATG GTGTCCTATA ATGCAGATCA GTGTATTGTC 
ATCAGTGGAG AAAGCGGCGC TGGGAAAACA GAGAGTGCTC ACCTGCTAGT CCAACAGCTG 
ACTGTCCTTG GAAAGGCCAA CAATCGGACC CTCCAGGAGA AGATTTTGCT GGTTAACAGT 
CTGGTCGAGG CATTCGGAAA TGCCTGCACT GTGATCAATG ATAACTCCAG TCGCTTTGGC 
AAATATCTGG AGATGAAGTT CACCTGTGGT GGGACAGTAG TGGGAGCTCA GATCTCTGAT 
TATCTGCTGG AGAAGTCTCG AGTCGTCCAC CATGCAGTAG GGGAACGCAA CTTCCACATC 
TTCTATTATA TCTACGCCGG ACTGGCAGAG AGGAAGAAAC TCGGCCATTA CAAGCTGTCA 
GACAGCAAAA CTCCAAGGTA TCTCCACAAT GAGAATGTTA AATTAGTGCC TGACATTGTG 
GGCAATGCCT TCTATAAGGA GCAGTTTGAG ACTGTGGAGC AGTGCTTCAA AGTCATCGGC 
TTCACCCTAG AGGAGCTCGG GAGTGTTTAC GGCATCTTAG CTGGGATCCT GAATACAGGA 
GACGTCGAGT TCACGTCAGT GGCTTCAGAA CATCAAACGG ACAAAAGCAA CATTTCCAAC 
ATGGCAGTAC TAGAGAGTGC TGCATCGCTG CTGTGTATCC GTGCAGATGA GCTACAGGAG 
GCTCTGACAT CTCATTGTGT GGTCACTCGG GGTGAAACCA TCGTCAGGTC AAACACAGTG 
GAGAAGGCCA CAGAGGTGCG GGACGCCATG GGTAAAGCCC TATATGGACG CCTGTTCAGC 
TGGATTGTCA ACCGCATCAA CTCTCTGCTC AAACCAGACA ACCAATCAGG GGAGGACGAC 
AAGGGTCTGA ACATCGGCAT CCTGGACATC TTCGGCTTTG AAAACTTTAA GAGGAACTCT 
TTCGAACAGC TCTGCATCAA CATCGCCAAT GAGCAGATCC AGTTCTACTT CAACCAGCAC 
ATCTTCGCCT GGGAACAGGA TGAGTATCTG AATGAAGATG TAGACGCTCG AGTAATTGAG 
TATGAAGACA ACCGGCCGCT GCTGGACCTG TTCCTTCAGA AGCCCATGGG CATGTTATCG 
CTGCTGGACG AGGAGAGTCG CTTTCCTCAG GCAACTGACC AGACCCTCGT CGAGAAATTT 
GAGGATAACT TAAAGTCCAA AAACTTCTGG AGACCAAAGA GGGTTGACCT GGGCTTTGGA 
ATCCATCACT ATGCAGGCAA GGTAATCTAT AATGCCGGCG GCTTCCTGGC TAAGAACAGA 
GACGCTTTGC CTGCTGATAT AGTTCTTCTG CTGAGGTCAT CAGAGAATGA ACTGACCCGT 
AAACTGGTTA CGCATCCGCT CACTAAAACA GGTAATTTGG CGCACACTAA GGGCAAAGCT 
GTGGGCTCTG CTCGGATGGG CACACAACAA TCACAGCGCT TTGCCAAGTT GGATTGCCTT 
TTGTTGTTCA GTTCTGTATC ATTCAGTGAG TCTTCCTCTC GGAAATTAAC TCCACCCATT 
GACAAATCAA TGCTTGCCTT GCCTGTTGAC TCTGTACAAA CGACGGAGAG CACTGGAGAC 
ACCACACATC ACCCCAGGGA GACCACCAAC ATGAAGACTC AAACTGTAGC GTCGTACTTC 
AGATATTCTC TAATGGACCT GCTATCAAAG ATGGTGGCAG GCCAGCCTCA TTTTGTTCGA 
TGCATAAAAC CCAACAATGA TCGTCAAGCT CACAAGTTTG ACAGGGAAAA GGTTCTGATC 
CAACTACGCT ACACTGGTAT TCTAGAGACG GCCAAGATCC GCAGACAAGG CTACTCTCAT 
CGCATTCTCT TCAACAACTT CATCGAAAGG TACTCCATAT TAGCATTTAG AGCTAATGAA 
GAGCCAGCAA TCAGTCCTGA GACGTGTGCG GTTATTTTAG AGAAGGCCAA ACTGGAGAAC 
TGGATTTTGG GCAAGACAAA GGTCTTTCTG AAGTATTATC ATGTGGAGCA GCTGAATCTG 
ATGGTCAGGC AGACCACTGA CCGGATTGTT CTAGTTCAGG CATATGTACG GGGCTGGATG 
GCATTCAAGC GATACTGTTT GCTGAAGGAA AAGAGAGAAC AAAGCGCTGT GGTGTTACAA 
TCAGCATACA GAGGACACAA GGCTCGGAAA AGTATAGCTG ATGACAGAAG TAAAGCAAAG 
TCTGAAATAG TTTTCACCCA ACTTCAAGCT TGGTGCAGAG GATATTTGGC AAGGCAGAAT 
TACAAGGAGC TACTAGATGA GAAGAACAAA GCTGCTGCAA AGATCCAGGC CCATTACAGG 
GGACAAAAAG AGAGGAAGAG TTTCCAGAGG AAAAAGGAAG CCATGCAGAA GGAAAACACA 
GAGAAGACAG AAAGAGTGAC AGAGGTCCCT TTAGAAGCAG ACGATCCTGT TTCAGAGGAA 
CCGCTACAGG AAGGAGGTGA AGTGGAAATG GATGATGCTG ATGAAAAGGC AGCTGTGGTC 
CTCCAGAGCA ACTACCGAGG ATACAGAGAA AGGAAGAAAT TCAAGGAACG TCAGGAACGG 
AATAAGACCT TGAGCGGAGA TGAGCTGGAT GCTTCCTCAT CCACCCCTGA AGAAATGACT 
TCTGGAGAAG ATCTGGAAGC TACACGAGAG GACACGCAGG AGAATGTTGA GAAAGAGGGG 
AATGAGGTTG AGGAAGGTGG AGGAGATGAA GACCCTGAGC AGGAAGCCAA GGCAGCCACT 
GTTCTCCAGA GCAACTTCAG AGGTCATAAG GAACGTAAAA GACTCCAAGA GGAAGGAAAG 
ATACCAGTTA GAGGAAAGAA GGAGATTAAG AGTCCTACAG ATGAGGAGGA GCTTCCTATT 
CCAGATCCAC CGCCCCCTGA GGAATTGATG GAGTTTTCAG AGGAAATGGA ATTTACTGAA 
GAAAATCCAC CAGGAGAAGA AGACGAGGCC AAAGCTGCTG TGGTCCTTCA GAGCAACTTC 
CGTGGCCACA AAGAAAGGAA AAGACTCCAA GAGGAAGGGA AAATCCCAAA GAAAAAGACT 
AAAGAGGAAA TGGATGATCC AGGAGCTTCA GAACCAGACA TTGCCATTGA GGAGCTTCCA 
CCGCCTCCTT TAGAACTTGA ACATGATCAA GAGCTAGACT CTAGTCCATC AGGAGGAGAT 
GAGGAACACG CTGCAACTGT TTTACAAAGC AATTTCAGAG GACACCGGGA GAGAAAGAGA 
CTGAGAGCTG AGAAAGAAGA GGCAACAGGA GGAGGGCAAA GCACTGGAGA TGTGGAGGAG 
CAGGAGAACA ATGACGTTCT AGACATTACA GATGTTCAGC TGGAACGAAG GGAAGAAGAG 
GAGGAAGGAC AATACGATGA GGAGCAGGCA GCCATTAAAA TCCAGAGCAA TTTTAGAGGC 
TATAAAGACC GAAAGAACCT GAAAGCTACA TCTCAAAAAC AAGATGAGGA AGTGGAGTCA 
CTCTTCAAAC AGGTATCCAA GGCCTCTGAA GACTACCTTT CCCTTCAGAA GAAGTTAAAT 
GAAATCATCC TCGCCCATCA GCTTAACCCC AGCAAAAATG GCATGTTTGT GAAAGGACAA 
CCCATGAATG GCTACATGGG CTACTACCAG CCACCAGCTG ACACAAAGCA GTCTCGAACA 
CCACGCCGCG CACAACAACC CAAGACCCTG AACACGCCGG AGGACTCCAC ATACTACACA 
CTTATTCATA GGTCTGTCCA GGACGATAAA AGGAGGCCGC GGAAACAAAG CCCAAAAAAG 
TTGGACAACG ATGACCAATA CTACCAGGGT TTGTCCAGCA GTAAATCCAC CAGCCTTCCC 
AATGAACGAC GCCGGTCGAG CAAGCGGAGG GCTTCTGATG AAAGACGCTC CTCTGAAAGA 
CCACTCTCTG ATGTCCCCAA ATCTGAAATC CCCAAAGACC CATTGGAGAA GAGGCACTCG 
TTCTCTAAAA TGCCCTCTAC AGAGAGTCAA AGCGAGGACA ACCCCTACGA CTACAGGAAG 
ATACTGAGAA AGACCTCCCA GAGACGACGA CTAATAACAC AGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991142.2
CDS	102..5429
Translation	MFPRSGKSIIFDSFPDPSDTWEIIETIGKGTYGKVYKVHNKVDGSKAAVKILDPIHDIDEEIEAEYNILKALSDHPNVVKFFGMFYKKDVKTGDQLWLVLELCNGGSVTDLAKGMLKRGDRMEEPIIAFILHEALMGLQHLHVNKTIHRDVKGNNILLTTDGGIKLVDFGVSAQLTNTRLRRNTSVGTPFWMAPEVIACEQQLDSTYDERCDVWSLGITAIELGDGDPPLAELHPMRALFKIPRNPPPTLHQPELWSNDFNDFIRKCLIKDFELRPNVLDLLQHVFIKQIVGREKTLQKQLMELIDLNQQMGIIEKTSLLGKSDRDSDSRHERIHTKKNSYMKSQSQTCDDADDLATLEVLDENTITEQLQRRYTKEQIYTYVGDILIAVNPFHKTELYTPEHTKSYVGAKRTSNPPHIYAVADIAYQSMVSYNADQCIVISGESGAGKTESAHLLVQQLTVLGKANNRTLQEKILLVNSLVEAFGNACTVINDNSSRFGKYLEMKFTCGGTVVGAQISDYLLEKSRVVHHAVGERNFHIFYYIYAGLAERKKLGHYKLSDSKTPRYLHNENVKLVPDIVGNAFYKEQFETVEQCFKVIGFTLEELGSVYGILAGILNTGDVEFTSVASEHQTDKSNISNMAVLESAASLLCIRADELQEALTSHCVVTRGETIVRSNTVEKATEVRDAMGKALYGRLFSWIVNRINSLLKPDNQSGEDDKGLNIGILDIFGFENFKRNSFEQLCINIANEQIQFYFNQHIFAWEQDEYLNEDVDARVIEYEDNRPLLDLFLQKPMGMLSLLDEESRFPQATDQTLVEKFEDNLKSKNFWRPKRVDLGFGIHHYAGKVIYNAGGFLAKNRDALPADIVLLLRSSENELTRKLVTHPLTKTGNLAHTKGKAVGSARMGTQQSQRFAKLDCLLLFSSVSFSESSSRKLTPPIDKSMLALPVDSVQTTESTGDTTHHPRETTNMKTQTVASYFRYSLMDLLSKMVAGQPHFVRCIKPNNDRQAHKFDREKVLIQLRYTGILETAKIRRQGYSHRILFNNFIERYSILAFRANEEPAISPETCAVILEKAKLENWILGKTKVFLKYYHVEQLNLMVRQTTDRIVLVQAYVRGWMAFKRYCLLKEKREQSAVVLQSAYRGHKARKSIADDRSKAKSEIVFTQLQAWCRGYLARQNYKELLDEKNKAAAKIQAHYRGQKERKSFQRKKEAMQKENTEKTERVTEVPLEADDPVSEEPLQEGGEVEMDDADEKAAVVLQSNYRGYRERKKFKERQERNKTLSGDELDASSSTPEEMTSGEDLEATREDTQENVEKEGNEVEEGGGDEDPEQEAKAATVLQSNFRGHKERKRLQEEGKIPVRGKKEIKSPTDEEELPIPDPPPPEELMEFSEEMEFTEENPPGEEDEAKAAVVLQSNFRGHKERKRLQEEGKIPKKKTKEEMDDPGASEPDIAIEELPPPPLELEHDQELDSSPSGGDEEHAATVLQSNFRGHRERKRLRAEKEEATGGGQSTGDVEEQENNDVLDITDVQLERREEEEEGQYDEEQAAIKIQSNFRGYKDRKNLKATSQKQDEEVESLFKQVSKASEDYLSLQKKLNEIILAHQLNPSKNGMFVKGQPMNGYMGYYQPPADTKQSRTPRRAQQPKTLNTPEDSTYYTLIHRSVQDDKRRPRKQSPKKLDNDDQYYQGLSSSKSTSLPNERRRSSKRRASDERRSSERPLSDVPKSEIPKDPLEKRHSFSKMPSTESQSEDNPYDYRKILRKTSQRRRLITQP

Target ORF information:

RefSeq Version	NM_205579.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin IIIA (myo3a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205579.2

ORF Insert Sequence:

ATGTTTCCTC GCTCAGGGAA ATCCATCATC TTTGACAGCT TCCCAGATCC GTCTGACACC 
TGGGAGATCA TTGAGACTAT TGGCAAAGGG ACTTATGGGA AAGTTTACAA AGTGCATAAT 
AAGGTCGATG GCAGCAAAGC CGCAGTCAAA ATACTGGATC CAATCCATGA CATCGATGAA 
GAAATTGAAG CTGAGTATAA CATCCTGAAA GCTCTTTCAG ACCATCCCAA TGTTGTTAAA 
TTCTTTGGGA TGTTCTACAA GAAGGATGTC AAGACTGGAG ACCAGCTATG GCTAGTACTA 
GAGCTGTGTA ACGGAGGGTC TGTCACAGAT CTGGCCAAAG GTATGCTAAA AAGAGGAGAT 
CGCATGGAGG AACCCATCAT AGCCTTTATT CTACATGAAG CGCTCATGGG TCTTCAACAT 
TTGCACGTCA ATAAGACCAT CCATCGAGAC GTCAAGGGCA ACAACATCCT GCTCACCACG 
GACGGAGGAA TCAAGCTGGT TGACTTTGGG GTTTCAGCCC AGCTCACAAA CACCCGACTA 
AGGAGGAACA CTTCGGTGGG AACGCCCTTC TGGATGGCTC CTGAGGTCAT CGCGTGTGAG 
CAGCAGCTGG ACTCCACATA TGATGAACGC TGTGATGTTT GGTCATTGGG AATCACAGCC 
ATAGAGCTAG GTGATGGAGA CCCGCCTCTC GCTGAACTCC ATCCAATGAG AGCTCTCTTC 
AAAATACCAA GAAATCCACC ACCCACTCTA CACCAGCCAG AGCTCTGGTC AAACGACTTT 
AACGATTTCA TACGCAAGTG TCTGATAAAG GATTTTGAGC TCAGGCCCAA TGTGCTTGAC 
CTTCTTCAGC ATGTCTTCAT CAAACAGATT GTGGGCCGAG AGAAGACGCT GCAGAAGCAG 
CTGATGGAGC TAATCGACCT CAACCAGCAA ATGGGCATCA TTGAGAAAAC AAGCCTTCTC 
GGAAAATCTG ACAGGGACAG TGACTCCAGG CATGAGCGTA TCCACACCAA GAAAAACAGC 
TACATGAAGT CTCAAAGTCA AACGTGTGAC GACGCTGATG ATCTGGCAAC CCTAGAGGTT 
CTAGATGAAA ACACAATCAC CGAACAGCTA CAGAGACGTT ACACAAAGGA GCAGATCTAC 
ACCTACGTCG GAGACATCCT TATAGCGGTC AATCCATTTC ACAAAACCGA GTTATACACA 
CCTGAGCATA CTAAGAGTTA TGTTGGCGCC AAACGAACAT CAAACCCCCC ACATATATAT 
GCAGTGGCAG ACATTGCGTA TCAGTCCATG GTGTCCTATA ATGCAGATCA GTGTATTGTC 
ATCAGTGGAG AAAGCGGCGC TGGGAAAACA GAGAGTGCTC ACCTGCTAGT CCAACAGCTG 
ACTGTCCTTG GAAAGGCCAA CAATCGGACC CTCCAGGAGA AGATTTTGCT GGTTAACAGT 
CTGGTCGAGG CATTCGGAAA TGCCTGCACT GTGATCAATG ATAACTCCAG TCGCTTTGGC 
AAATATCTGG AGATGAAGTT CACCTGTGGT GGGACAGTAG TGGGAGCTCA GATCTCTGAT 
TATCTGCTGG AGAAGTCTCG AGTCGTCCAC CATGCAGTAG GGGAACGCAA CTTCCACATC 
TTCTATTATA TCTACGCCGG ACTGGCAGAG AGGAAGAAAC TCGGCCATTA CAAGCTGTCA 
GACAGCAAAA CTCCAAGGTA TCTCCACAAT GAGAATGTTA AATTAGTGCC TGACATTGTG 
GGCAATGCCT TCTATAAGGA GCAGTTTGAG ACTGTGGAGC AGTGCTTCAA AGTCATCGGC 
TTCACCCTAG AGGAGCTCGG GAGTGTTTAC GGCATCTTAG CTGGGATCCT GAATACAGGA 
GACGTCGAGT TCACGTCAGT GGCTTCAGAA CATCAAACGG ACAAAAGCAA CATTTCCAAC 
ATGGCAGTAC TAGAGAGTGC TGCATCGCTG CTGTGTATCC GTGCAGATGA GCTACAGGAG 
GCTCTGACAT CTCATTGTGT GGTCACTCGG GGTGAAACCA TCGTCAGGTC AAACACAGTG 
GAGAAGGCCA CAGAGGTGCG GGACGCCATG GGTAAAGCCC TATATGGACG CCTGTTCAGC 
TGGATTGTCA ACCGCATCAA CTCTCTGCTC AAACCAGACA ACCAATCAGG GGAGGACGAC 
AAGGGTCTGA ACATCGGCAT CCTGGACATC TTCGGCTTTG AAAACTTTAA GAGGAACTCT 
TTCGAACAGC TCTGCATCAA CATCGCCAAT GAGCAGATCC AGTTCTACTT CAACCAGCAC 
ATCTTCGCCT GGGAACAGGA TGAGTATCTG AATGAAGATG TAGACGCTCG AGTAATTGAG 
TATGAAGACA ACCGGCCGCT GCTGGACCTG TTCCTTCAGA AGCCCATGGG CATGTTATCG 
CTGCTGGACG AGGAGAGTCG CTTTCCTCAG GCAACTGACC AGACCCTCGT CGAGAAATTT 
GAGGATAACT TAAAGTCCAA AAACTTCTGG AGACCAAAGA GGGTTGACCT GGGCTTTGGA 
ATCCATCACT ATGCAGGCAA GGTAATCTAT AATGCCGGCG GCTTCCTGGC TAAGAACAGA 
GACGCTTTGC CTGCTGATAT AGTTCTTCTG CTGAGGTCAT CAGAGAATGA ACTGACCCGT 
AAACTGGTTA CGCATCCGCT CACTAAAACA GGTAATTTGG CGCACACTAA GGGCAAAGCT 
GTGGGCTCTG CTCGGATGGG CACACAACAA TCACAGCGCT TTGCCAAGTT GGATTGCCTT 
TTGTTGTTCA GTTCTGTATC ATTCAGTGAG TCTTCCTCTC GGAAATTAAC TCCACCCATT 
GACAAATCAA TGCTTGCCTT GCCTGTTGAC TCTGTACAAA CGACGGAGAG CACTGGAGAC 
ACCACACATC ACCCCAGGGA GACCACCAAC ATGAAGACTC AAACTGTAGC GTCGTACTTC 
AGATATTCTC TAATGGACCT GCTATCAAAG ATGGTGGCAG GCCAGCCTCA TTTTGTTCGA 
TGCATAAAAC CCAACAATGA TCGTCAAGCT CACAAGTTTG ACAGGGAAAA GGTTCTGATC 
CAACTACGCT ACACTGGTAT TCTAGAGACG GCCAAGATCC GCAGACAAGG CTACTCTCAT 
CGCATTCTCT TCAACAACTT CATCGAAAGG TACTCCATAT TAGCATTTAG AGCTAATGAA 
GAGCCAGCAA TCAGTCCTGA GACGTGTGCG GTTATTTTAG AGAAGGCCAA ACTGGAGAAC 
TGGATTTTGG GCAAGACAAA GGTCTTTCTG AAGTATTATC ATGTGGAGCA GCTGAATCTG 
ATGGTCAGGC AGACCACTGA CCGGATTGTT CTAGTTCAGG CATATGTACG GGGCTGGATG 
GCATTCAAGC GATACTGTTT GCTGAAGGAA AAGAGAGAAC AAAGCGCTGT GGTGTTACAA 
TCAGCATACA GAGGACACAA GGCTCGGAAA AGTATAGCTG ATGACAGAAG TAAAGCAAAG 
TCTGAAATAG TTTTCACCCA ACTTCAAGCT TGGTGCAGAG GATATTTGGC AAGGCAGAAT 
TACAAGGAGC TACTAGATGA GAAGAACAAA GCTGCTGCAA AGATCCAGGC CCATTACAGG 
GGACAAAAAG AGAGGAAGAG TTTCCAGAGG AAAAAGGAAG CCATGCAGAA GGAAAACACA 
GAGAAGACAG AAAGAGTGAC AGAGGTCCCT TTAGAAGCAG ACGATCCTGT TTCAGAGGAA 
CCGCTACAGG AAGGAGGTGA AGTGGAAATG GATGATGCTG ATGAAAAGGC AGCTGTGGTC 
CTCCAGAGCA ACTACCGAGG ATACAGAGAA AGGAAGAAAT TCAAGGAACG TCAGGAACGG 
AATAAGACCT TGAGCGGAGA TGAGCTGGAT GCTTCCTCAT CCACCCCTGA AGAAATGACT 
TCTGGAGAAG ATCTGGAAGC TACACGAGAG GACACGCAGG AGAATGTTGA GAAAGAGGGG 
AATGAGGTTG AGGAAGGTGG AGGAGATGAA GACCCTGAGC AGGAAGCCAA GGCAGCCACT 
GTTCTCCAGA GCAACTTCAG AGGTCATAAG GAACGTAAAA GACTCCAAGA GGAAGGAAAG 
ATACCAGTTA GAGGAAAGAA GGAGATTAAG AGTCCTACAG ATGAGGAGGA GCTTCCTATT 
CCAGATCCAC CGCCCCCTGA GGAATTGATG GAGTTTTCAG AGGAAATGGA ATTTACTGAA 
GAAAATCCAC CAGGAGAAGA AGACGAGGCC AAAGCTGCTG TGGTCCTTCA GAGCAACTTC 
CGTGGCCACA AAGAAAGGAA AAGACTCCAA GAGGAAGGGA AAATCCCAAA GAAAAAGACT 
AAAGAGGAAA TGGATGATCC AGGAGCTTCA GAACCAGACA TTGCCATTGA GGAGCTTCCA 
CCGCCTCCTT TAGAACTTGA ACATGATCAA GAGCTAGACT CTAGTCCATC AGGAGGAGAT 
GAGGAACACG CTGCAACTGT TTTACAAAGC AATTTCAGAG GACACCGGGA GAGAAAGAGA 
CTGAGAGCTG AGAAAGAAGA GGCAACAGGA GGAGGGCAAA GCACTGGAGA TGTGGAGGAG 
CAGGAGAACA ATGACGTTCT AGACATTACA GATGTTCAGC TGGAACGAAG GGAAGAAGAG 
GAGGAAGGAC AATACGATGA GGAGCAGGCA GCCATTAAAA TCCAGAGCAA TTTTAGAGGC 
TATAAAGACC GAAAGAACCT GAAAGCTACA TCTCAAAAAC AAGATGAGGA AGTGGAGTCA 
CTCTTCAAAC AGGTATCCAA GGCCTCTGAA GACTACCTTT CCCTTCAGAA GAAGTTAAAT 
GAAATCATCC TCGCCCATCA GCTTAACCCC AGCAAAAATG GCATGTTTGT GAAAGGACAA 
CCCATGAATG GCTACATGGG CTACTACCAG CCACCAGCTG ACACAAAGCA GTCTCGAACA 
CCACGCCGCG CACAACAACC CAAGACCCTG AACACGCCGG AGGACTCCAC ATACTACACA 
CTTATTCATA GGTCTGTCCA GGACGATAAA AGGAGGCCGC GGAAACAAAG CCCAAAAAAG 
TTGGACAACG ATGACCAATA CTACCAGGGT TTGTCCAGCA GTAAATCCAC CAGCCTTCCC 
AATGAACGAC GCCGGTCGAG CAAGCGGAGG GCTTCTGATG AAAGACGCTC CTCTGAAAGA 
CCACTCTCTG ATGTCCCCAA ATCTGAAATC CCCAAAGACC CATTGGAGAA GAGGCACTCG 
TTCTCTAAAA TGCCCTCTAC AGAGAGTCAA AGCGAGGACA ACCCCTACGA CTACAGGAAG 
ATACTGAGAA AGACCTCCCA GAGACGACGA CTAATAACAC AGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo3a ( NM_001362412.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001349341.1 Danio rerio myosin IIIA (myo3a), transcript variant 2, mRNA.

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CloneID	ODa159841
Clone ID Related Accession (Same CDS sequence)	NM_001362412.1
Accession Version	NM_001362412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570809600000
Organism	Danio rerio(zebrafish)
Product	myosin-IIIa isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX530066.21. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC162575.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTTCCTC GCTCAGGGAA ATCCATCATC TTTGACAGCT TCCCAGATCC GTCTGACACC 
TGGGAGATCA TTGAGACTAT TGGCAAAGGG ACTTATGGGA AAGTTTACAA AGTGCATAAT 
AAGGTCGATG GCAGCAAAGC CGCAGTCAAA ATACTGGATC CAATCCATGA CATCGATGAA 
GAAATTGAAG CTGAGTATAA CATCCTGAAA GCTCTTTCAG ACCATCCCAA TGTTGTTAAA 
TTCTTTGGGA TGTTCTACAA GAAGGATGTC AAGACTGGAG ACCAGCTATG GCTAGTACTA 
GAGCTGTGTA ACGGAGGGTC TGTCACAGAT CTGGCCAAAG GTATGCTAAA AAGAGGAGAT 
CGCATGGAGG AACCCATCAT AGCCTTTATT CTACATGAAG CGCTCATGGG TCTTCAACAT 
TTGCACGTCA ATAAGACCAT CCATCGAGAC GTCAAGGGCA ACAACATCCT GCTCACCACG 
GACGGAGGAA TCAAGCTGGT TGACTTTGGG GTTTCAGCCC AGCTCACAAA CACCCGACTA 
AGGAGGAACA CTTCGGTGGG AACGCCCTTC TGGATGGCTC CTGAGGTCAT CGCGTGTGAG 
CAGCAGCTGG ACTCCACATA TGATGAACGC TGTGATGTTT GGTCATTGGG AATCACAGCC 
ATAGAGCTAG GTGATGGAGA CCCGCCTCTC GCTGAACTCC ATCCAATGAG AGCTCTCTTC 
AAAATACCAA GAAATCCACC ACCCACTCTA CACCAGCCAG AGCTCTGGTC AAACGACTTT 
AACGATTTCA TACGCAAGTG TCTGATAAAG GATTTTGAGC TCAGGCCCAA TGTGCTTGAC 
CTTCTTCAGC ATGTCTTCAT CAAACAGATT GTGGGCCGAG AGAAGACGCT GCAGAAGCAG 
CTGATGGAGC TAATCGACCT CAACCAGCAA ATGGGCATCA TTGAGAAAAC AAGGCATGAG 
CGTATCCACA CCAAGAAAAA CAGCTACATG AAGTCTCAAA GTCAAACGTG TGACGACGCT 
GATGATCTGG CAACCCTAGA GGTTCTAGAT GAAAACACAA TCACCGAACA GCTACAGAGA 
CGTTACACAA AGGAGCAGAT CTACACCTAC GTCGGAGACA TCCTTATAGC GGTCAATCCA 
TTTCACAAAA CCGAGTTATA CACACCTGAG CATACTAAGA GTTATGTTGG CGCCAAACGA 
ACATCAAACC CCCCACATAT ATATGCAGTG GCAGACATTG CGTATCAGTC CATGGTGTCC 
TATAATGCAG ATCAGTGTAT TGTCATCAGT GGAGAAAGCG GCGCTGGGAA AACAGAGAGT 
GCTCACCTGC TAGTCCAACA GCTGACTGTC CTTGGAAAGG CCAACAATCG GACCCTCCAG 
GAGAAGATTT TGCTGGTTAA CAGTCTGGTC GAGGCATTCG GAAATGCCTG CACTGTGATC 
AATGATAACT CCAGTCGCTT TGGCAAATAT CTGGAGATGA AGTTCACCTG TGGTGGGACA 
GTAGTGGGAG CTCAGATCTC TGATTATCTG CTGGAGAAGT CTCGAGTCGT CCACCATGCA 
GTAGGGGAAC GCAACTTCCA CATCTTCTAT TATATCTACG CCGGACTGGC AGAGAGGAAG 
AAACTCGGCC ATTACAAGCT GTCAGACAGC AAAACTCCAA GGTATCTCCA CAATGAGAAT 
GTTAAATTAG TGCCTGACAT TGTGGGCAAT GCCTTCTATA AGGAGCAGTT TGAGACTGTG 
GAGCAGTGCT TCAAAGTCAT CGGCTTCACC CTAGAGGAGC TCGGGAGTGT TTACGGCATC 
TTAGCTGGGA TCCTGAATAC AGGAGACGTC GAGTTCACGT CAGTGGCTTC AGAACATCAA 
ACGGACAAAA GCAACATTTC CAACATGGCA GTACTAGAGA GTGCTGCATC GCTGCTGTGT 
ATCCGTGCAG ATGAGCTACA GGAGGCTCTG ACATCTCATT GTGTGGTCAC TCGGGGTGAA 
ACCATCGTCA GGTCAAACAC AGTGGAGAAG GCCACAGAGG TGCGGGACGC CATGGGTAAA 
GCCCTATATG GACGCCTGTT CAGCTGGATT GTCAACCGCA TCAACTCTCT GCTCAAACCA 
GACAACCAAT CAGGGGAGGA CGACAAGGGT CTGAACATCG GCATCCTGGA CATCTTCGGC 
TTTGAAAACT TTAAGAGGAA CTCTTTCGAA CAGCTCTGCA TCAACATCGC CAATGAGCAG 
ATCCAGTTCT ACTTCAACCA GCACATCTTC GCCTGGGAAC AGGATGAGTA TCTGAATGAA 
GATGTAGACG CTCGAGTAAT TGAGTATGAA GACAACCGGC CGCTGCTGGA CCTGTTCCTT 
CAGAAGCCCA TGGGCATGTT ATCGCTGCTG GACGAGGAGA GTCGCTTTCC TCAGGCAACT 
GACCAGACCC TCGTCGAGAA ATTTGAGGAT AACTTAAAGT CCAAAAACTT CTGGAGACCA 
AAGAGGGTTG ACCTGGGCTT TGGAATCCAT CACTATGCAG GCAAGGTAAT CTATAATGCC 
GGCGGCTTCC TGGCTAAGAA CAGAGACGCT TTGCCTGCTG ATATAGTTCT TCTGCTGAGG 
TCATCAGAGA ATGAACTGAC CCGTAAACTG GTTACGCATC CGCTCACTAA AACAGGTAAT 
TTGGCGCACA CTAAGGGCAA AGCTGTGGGC TCTGCTCGGA TGGGCACACA ACAATCACAG 
CGCTTTGCCA AGTTGGATTG CCTTTTGTTG TTCAGTTCTG TATCATTCAG TGAGTCTTCC 
TCTCGGAAAT TAACTCCACC CATTGACAAA TCAATGCTTG CCTTGCCTGT TGACTCTGTA 
CAAACGACGG AGAGCACTGG AGACACCACA CATCACCCCA GGGAGACCAC CAACATGAAG 
ACTCAAACTG TAGCGTCGTA CTTCAGATAT TCTCTAATGG ACCTGCTATC AAAGATGGTG 
GCAGGCCAGC CTCATTTTGT TCGATGCATA AAACCCAACA ATGATCGTCA AGCTCACAAG 
TTTGACAGGG AAAAGGTTCT GATCCAACTA CGCTACACTG GTATTCTAGA GACGGCCAAG 
ATCCGCAGAC AAGGCTACTC TCATCGCATT CTCTTCAACA ACTTCATCGA AAGGTACTCC 
ATATTAGCAT TTAGAGCTAA TGAAGAGCCA GCAATCAGTC CTGAGACGTG TGCGGTTATT 
TTAGAGAAGG CCAAACTGGA GAACTGGATT TTGGGCAAGA CAAAGGTCTT TCTGAAGTAT 
TATCATGTGG AGCAGCTGAA TCTGATGGTC AGGCAGACCA CTGACCGGAT TGTTCTAGTT 
CAGGCATATG TACGGGGCTG GATGGCATTC AAGCGATACT GTTTGCTGAA GGAAAAGAGA 
GAACAAAGCG CTGTGGTGTT ACAATCAGCA TACAGAGGAC ACAAGGCTCG GAAAAGTATA 
GCTGATGACA GAAGTAAAGC AAAGTCTGAA ATAGTTTTCA CCCAACTTCA AGCTTGGTGC 
AGAGGATATT TGGCAAGGCA GAATTACAAG GAGCTACTAG ATGAGAAGAA CAAAGCTGCT 
GCAAAGATCC AGGCCCATTA CAGGGGACAA AAAGAGAGGA AGAGTTTCCA GAGGAAAAAG 
GAAGCCATGC AGAAGGAAAA CACAGAGAAG ACAGAAAGAG TGACAGAGGT CCCTTTAGAA 
GCAGACGATC CTGTTTCAGA GGAACCGCTA CAGGAAGGAG GTGAAGTGGA AATGGATGAT 
GCTGATGAAA AGGCAGCTGT GGTCCTCCAG AGCAACTACC GAGGATACAG AGAAAGGAAG 
AAATTCAAGG AACGTCAGGA ACGGAATAAG ACCTTGAGCG GAGATGAGCT GGATGCTTCC 
TCATCCACCC CTGAAGAAAT GACTTCTGGA GAAGATCTGG AAGCTACACG AGAGGACACG 
CAGGAGAATG TTGAGAAAGA GGGGAATGAG GTTGAGGAAG GTGGAGGAGA TGAAGACCCT 
GAGCAGGAAG CCAAGGCAGC CACTGTTCTC CAGAGCAACT TCAGAGGTCA TAAGGAACGT 
AAAAGACTCC AAGAGGAAGG AAAGATACCA GTTAGAGGAA AGAAGGAGAT TAAGAGTCCT 
ACAGATGAGG AGGAGCTTCC TATTCCAGAT CCACCGCCCC CTGAGGAATT GATGGAGTTT 
TCAGAGGAAA TGGAATTTAC TGAAGAAAAT CCACCAGGAG AAGAAGACGA GGCCAAAGCT 
GCTGTGGTCC TTCAGAGCAA CTTCCGTGGC CACAAAGAAA GGAAAAGACT CCAAGAGGAA 
GGGAAAATCC CAAAGAAAAA GACTAAAGAG GAAATGGATG ATCCAGGAGC TTCAGAACCA 
GACATTGCCA TTGAGGAGCT TCCACCGCCT CCTTTAGAAC TTGAACATGA TCAAGAGCTA 
GACTCTAGTC CATCAGGAGG AGATGAGGAA CACGCTGCAA CTGTTTTACA AAGCAATTTC 
AGAGGACACC GGGAGAGAAA GAGACTGAGA GCTGAGAAAG AAGAGGCAAC AGGAGGAGGG 
CAAAGCACTG GAGATGTGGA GGAGCAGGAG AACAATGACG TTCTAGACAT TACAGATGTT 
CAGCTGGAAC GAAGGGAAGA AGAGGAGGAA GGACAATACG ATGAGGAGCA GGCAGCCATT 
AAAATCCAGA GCAATTTTAG AGGCTATAAA GACCGAAAGA ACCTGAAAGC TACATCTCAA 
AAACAAGATG AGGAAGTGGA GTCACTCTTC AAACAGGTAT CCAAGGCCTC TGAAGACTAC 
CTTTCCCTTC AGAAGAAGTT AAATGAAATC ATCCTCGCCC ATCAGCTTAA CCCCAGCAAA 
AATGGCATGT TTGTGAAAGG ACAACCCATG AATGGCTACA TGGGCTACTA CCAGCCACCA 
GCTGACACAA AGCAGTCTCG AACACCACGC CGCGCACAAC AACCCAAGAC CCTGAACACG 
CCGGAGGACT CCACATACTA CACACTTATT CATAGGTCTG TCCAGGACGA TAAAAGGAGG 
CCGCGGAAAC AAAGCCCAAA AAAGTTGGAC AACGATGACC AATACTACCA GGGTTTGTCC 
AGCAGTAAAT CCACCAGCCT TCCCAATGAA CGACGCCGGT CGAGCAAGCG GAGGGCTTCT 
GATGAAAGAC GCTCCTCTGA AAGACCACTC TCTGATGTCC CCAAATCTGA AATCCCCAAA 
GACCCATTGG AGAAGAGGCA CTCGTTCTCT AAAATGCCCT CTACAGAGAG TCAAAGCGAG 
GACAACCCCT ACGACTACAG GAAGATACTG AGAAAGACCT CCCAGAGACG ACGACTAATA 
ACACAGCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349341.1
CDS	102..5393
Translation	MFPRSGKSIIFDSFPDPSDTWEIIETIGKGTYGKVYKVHNKVDGSKAAVKILDPIHDIDEEIEAEYNILKALSDHPNVVKFFGMFYKKDVKTGDQLWLVLELCNGGSVTDLAKGMLKRGDRMEEPIIAFILHEALMGLQHLHVNKTIHRDVKGNNILLTTDGGIKLVDFGVSAQLTNTRLRRNTSVGTPFWMAPEVIACEQQLDSTYDERCDVWSLGITAIELGDGDPPLAELHPMRALFKIPRNPPPTLHQPELWSNDFNDFIRKCLIKDFELRPNVLDLLQHVFIKQIVGREKTLQKQLMELIDLNQQMGIIEKTRHERIHTKKNSYMKSQSQTCDDADDLATLEVLDENTITEQLQRRYTKEQIYTYVGDILIAVNPFHKTELYTPEHTKSYVGAKRTSNPPHIYAVADIAYQSMVSYNADQCIVISGESGAGKTESAHLLVQQLTVLGKANNRTLQEKILLVNSLVEAFGNACTVINDNSSRFGKYLEMKFTCGGTVVGAQISDYLLEKSRVVHHAVGERNFHIFYYIYAGLAERKKLGHYKLSDSKTPRYLHNENVKLVPDIVGNAFYKEQFETVEQCFKVIGFTLEELGSVYGILAGILNTGDVEFTSVASEHQTDKSNISNMAVLESAASLLCIRADELQEALTSHCVVTRGETIVRSNTVEKATEVRDAMGKALYGRLFSWIVNRINSLLKPDNQSGEDDKGLNIGILDIFGFENFKRNSFEQLCINIANEQIQFYFNQHIFAWEQDEYLNEDVDARVIEYEDNRPLLDLFLQKPMGMLSLLDEESRFPQATDQTLVEKFEDNLKSKNFWRPKRVDLGFGIHHYAGKVIYNAGGFLAKNRDALPADIVLLLRSSENELTRKLVTHPLTKTGNLAHTKGKAVGSARMGTQQSQRFAKLDCLLLFSSVSFSESSSRKLTPPIDKSMLALPVDSVQTTESTGDTTHHPRETTNMKTQTVASYFRYSLMDLLSKMVAGQPHFVRCIKPNNDRQAHKFDREKVLIQLRYTGILETAKIRRQGYSHRILFNNFIERYSILAFRANEEPAISPETCAVILEKAKLENWILGKTKVFLKYYHVEQLNLMVRQTTDRIVLVQAYVRGWMAFKRYCLLKEKREQSAVVLQSAYRGHKARKSIADDRSKAKSEIVFTQLQAWCRGYLARQNYKELLDEKNKAAAKIQAHYRGQKERKSFQRKKEAMQKENTEKTERVTEVPLEADDPVSEEPLQEGGEVEMDDADEKAAVVLQSNYRGYRERKKFKERQERNKTLSGDELDASSSTPEEMTSGEDLEATREDTQENVEKEGNEVEEGGGDEDPEQEAKAATVLQSNFRGHKERKRLQEEGKIPVRGKKEIKSPTDEEELPIPDPPPPEELMEFSEEMEFTEENPPGEEDEAKAAVVLQSNFRGHKERKRLQEEGKIPKKKTKEEMDDPGASEPDIAIEELPPPPLELEHDQELDSSPSGGDEEHAATVLQSNFRGHRERKRLRAEKEEATGGGQSTGDVEEQENNDVLDITDVQLERREEEEEGQYDEEQAAIKIQSNFRGYKDRKNLKATSQKQDEEVESLFKQVSKASEDYLSLQKKLNEIILAHQLNPSKNGMFVKGQPMNGYMGYYQPPADTKQSRTPRRAQQPKTLNTPEDSTYYTLIHRSVQDDKRRPRKQSPKKLDNDDQYYQGLSSSKSTSLPNERRRSSKRRASDERRSSERPLSDVPKSEIPKDPLEKRHSFSKMPSTESQSEDNPYDYRKILRKTSQRRRLITQP

Target ORF information:

RefSeq Version	NM_001362412.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin IIIA (myo3a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362412.1

ORF Insert Sequence:

ATGTTTCCTC GCTCAGGGAA ATCCATCATC TTTGACAGCT TCCCAGATCC GTCTGACACC 
TGGGAGATCA TTGAGACTAT TGGCAAAGGG ACTTATGGGA AAGTTTACAA AGTGCATAAT 
AAGGTCGATG GCAGCAAAGC CGCAGTCAAA ATACTGGATC CAATCCATGA CATCGATGAA 
GAAATTGAAG CTGAGTATAA CATCCTGAAA GCTCTTTCAG ACCATCCCAA TGTTGTTAAA 
TTCTTTGGGA TGTTCTACAA GAAGGATGTC AAGACTGGAG ACCAGCTATG GCTAGTACTA 
GAGCTGTGTA ACGGAGGGTC TGTCACAGAT CTGGCCAAAG GTATGCTAAA AAGAGGAGAT 
CGCATGGAGG AACCCATCAT AGCCTTTATT CTACATGAAG CGCTCATGGG TCTTCAACAT 
TTGCACGTCA ATAAGACCAT CCATCGAGAC GTCAAGGGCA ACAACATCCT GCTCACCACG 
GACGGAGGAA TCAAGCTGGT TGACTTTGGG GTTTCAGCCC AGCTCACAAA CACCCGACTA 
AGGAGGAACA CTTCGGTGGG AACGCCCTTC TGGATGGCTC CTGAGGTCAT CGCGTGTGAG 
CAGCAGCTGG ACTCCACATA TGATGAACGC TGTGATGTTT GGTCATTGGG AATCACAGCC 
ATAGAGCTAG GTGATGGAGA CCCGCCTCTC GCTGAACTCC ATCCAATGAG AGCTCTCTTC 
AAAATACCAA GAAATCCACC ACCCACTCTA CACCAGCCAG AGCTCTGGTC AAACGACTTT 
AACGATTTCA TACGCAAGTG TCTGATAAAG GATTTTGAGC TCAGGCCCAA TGTGCTTGAC 
CTTCTTCAGC ATGTCTTCAT CAAACAGATT GTGGGCCGAG AGAAGACGCT GCAGAAGCAG 
CTGATGGAGC TAATCGACCT CAACCAGCAA ATGGGCATCA TTGAGAAAAC AAGGCATGAG 
CGTATCCACA CCAAGAAAAA CAGCTACATG AAGTCTCAAA GTCAAACGTG TGACGACGCT 
GATGATCTGG CAACCCTAGA GGTTCTAGAT GAAAACACAA TCACCGAACA GCTACAGAGA 
CGTTACACAA AGGAGCAGAT CTACACCTAC GTCGGAGACA TCCTTATAGC GGTCAATCCA 
TTTCACAAAA CCGAGTTATA CACACCTGAG CATACTAAGA GTTATGTTGG CGCCAAACGA 
ACATCAAACC CCCCACATAT ATATGCAGTG GCAGACATTG CGTATCAGTC CATGGTGTCC 
TATAATGCAG ATCAGTGTAT TGTCATCAGT GGAGAAAGCG GCGCTGGGAA AACAGAGAGT 
GCTCACCTGC TAGTCCAACA GCTGACTGTC CTTGGAAAGG CCAACAATCG GACCCTCCAG 
GAGAAGATTT TGCTGGTTAA CAGTCTGGTC GAGGCATTCG GAAATGCCTG CACTGTGATC 
AATGATAACT CCAGTCGCTT TGGCAAATAT CTGGAGATGA AGTTCACCTG TGGTGGGACA 
GTAGTGGGAG CTCAGATCTC TGATTATCTG CTGGAGAAGT CTCGAGTCGT CCACCATGCA 
GTAGGGGAAC GCAACTTCCA CATCTTCTAT TATATCTACG CCGGACTGGC AGAGAGGAAG 
AAACTCGGCC ATTACAAGCT GTCAGACAGC AAAACTCCAA GGTATCTCCA CAATGAGAAT 
GTTAAATTAG TGCCTGACAT TGTGGGCAAT GCCTTCTATA AGGAGCAGTT TGAGACTGTG 
GAGCAGTGCT TCAAAGTCAT CGGCTTCACC CTAGAGGAGC TCGGGAGTGT TTACGGCATC 
TTAGCTGGGA TCCTGAATAC AGGAGACGTC GAGTTCACGT CAGTGGCTTC AGAACATCAA 
ACGGACAAAA GCAACATTTC CAACATGGCA GTACTAGAGA GTGCTGCATC GCTGCTGTGT 
ATCCGTGCAG ATGAGCTACA GGAGGCTCTG ACATCTCATT GTGTGGTCAC TCGGGGTGAA 
ACCATCGTCA GGTCAAACAC AGTGGAGAAG GCCACAGAGG TGCGGGACGC CATGGGTAAA 
GCCCTATATG GACGCCTGTT CAGCTGGATT GTCAACCGCA TCAACTCTCT GCTCAAACCA 
GACAACCAAT CAGGGGAGGA CGACAAGGGT CTGAACATCG GCATCCTGGA CATCTTCGGC 
TTTGAAAACT TTAAGAGGAA CTCTTTCGAA CAGCTCTGCA TCAACATCGC CAATGAGCAG 
ATCCAGTTCT ACTTCAACCA GCACATCTTC GCCTGGGAAC AGGATGAGTA TCTGAATGAA 
GATGTAGACG CTCGAGTAAT TGAGTATGAA GACAACCGGC CGCTGCTGGA CCTGTTCCTT 
CAGAAGCCCA TGGGCATGTT ATCGCTGCTG GACGAGGAGA GTCGCTTTCC TCAGGCAACT 
GACCAGACCC TCGTCGAGAA ATTTGAGGAT AACTTAAAGT CCAAAAACTT CTGGAGACCA 
AAGAGGGTTG ACCTGGGCTT TGGAATCCAT CACTATGCAG GCAAGGTAAT CTATAATGCC 
GGCGGCTTCC TGGCTAAGAA CAGAGACGCT TTGCCTGCTG ATATAGTTCT TCTGCTGAGG 
TCATCAGAGA ATGAACTGAC CCGTAAACTG GTTACGCATC CGCTCACTAA AACAGGTAAT 
TTGGCGCACA CTAAGGGCAA AGCTGTGGGC TCTGCTCGGA TGGGCACACA ACAATCACAG 
CGCTTTGCCA AGTTGGATTG CCTTTTGTTG TTCAGTTCTG TATCATTCAG TGAGTCTTCC 
TCTCGGAAAT TAACTCCACC CATTGACAAA TCAATGCTTG CCTTGCCTGT TGACTCTGTA 
CAAACGACGG AGAGCACTGG AGACACCACA CATCACCCCA GGGAGACCAC CAACATGAAG 
ACTCAAACTG TAGCGTCGTA CTTCAGATAT TCTCTAATGG ACCTGCTATC AAAGATGGTG 
GCAGGCCAGC CTCATTTTGT TCGATGCATA AAACCCAACA ATGATCGTCA AGCTCACAAG 
TTTGACAGGG AAAAGGTTCT GATCCAACTA CGCTACACTG GTATTCTAGA GACGGCCAAG 
ATCCGCAGAC AAGGCTACTC TCATCGCATT CTCTTCAACA ACTTCATCGA AAGGTACTCC 
ATATTAGCAT TTAGAGCTAA TGAAGAGCCA GCAATCAGTC CTGAGACGTG TGCGGTTATT 
TTAGAGAAGG CCAAACTGGA GAACTGGATT TTGGGCAAGA CAAAGGTCTT TCTGAAGTAT 
TATCATGTGG AGCAGCTGAA TCTGATGGTC AGGCAGACCA CTGACCGGAT TGTTCTAGTT 
CAGGCATATG TACGGGGCTG GATGGCATTC AAGCGATACT GTTTGCTGAA GGAAAAGAGA 
GAACAAAGCG CTGTGGTGTT ACAATCAGCA TACAGAGGAC ACAAGGCTCG GAAAAGTATA 
GCTGATGACA GAAGTAAAGC AAAGTCTGAA ATAGTTTTCA CCCAACTTCA AGCTTGGTGC 
AGAGGATATT TGGCAAGGCA GAATTACAAG GAGCTACTAG ATGAGAAGAA CAAAGCTGCT 
GCAAAGATCC AGGCCCATTA CAGGGGACAA AAAGAGAGGA AGAGTTTCCA GAGGAAAAAG 
GAAGCCATGC AGAAGGAAAA CACAGAGAAG ACAGAAAGAG TGACAGAGGT CCCTTTAGAA 
GCAGACGATC CTGTTTCAGA GGAACCGCTA CAGGAAGGAG GTGAAGTGGA AATGGATGAT 
GCTGATGAAA AGGCAGCTGT GGTCCTCCAG AGCAACTACC GAGGATACAG AGAAAGGAAG 
AAATTCAAGG AACGTCAGGA ACGGAATAAG ACCTTGAGCG GAGATGAGCT GGATGCTTCC 
TCATCCACCC CTGAAGAAAT GACTTCTGGA GAAGATCTGG AAGCTACACG AGAGGACACG 
CAGGAGAATG TTGAGAAAGA GGGGAATGAG GTTGAGGAAG GTGGAGGAGA TGAAGACCCT 
GAGCAGGAAG CCAAGGCAGC CACTGTTCTC CAGAGCAACT TCAGAGGTCA TAAGGAACGT 
AAAAGACTCC AAGAGGAAGG AAAGATACCA GTTAGAGGAA AGAAGGAGAT TAAGAGTCCT 
ACAGATGAGG AGGAGCTTCC TATTCCAGAT CCACCGCCCC CTGAGGAATT GATGGAGTTT 
TCAGAGGAAA TGGAATTTAC TGAAGAAAAT CCACCAGGAG AAGAAGACGA GGCCAAAGCT 
GCTGTGGTCC TTCAGAGCAA CTTCCGTGGC CACAAAGAAA GGAAAAGACT CCAAGAGGAA 
GGGAAAATCC CAAAGAAAAA GACTAAAGAG GAAATGGATG ATCCAGGAGC TTCAGAACCA 
GACATTGCCA TTGAGGAGCT TCCACCGCCT CCTTTAGAAC TTGAACATGA TCAAGAGCTA 
GACTCTAGTC CATCAGGAGG AGATGAGGAA CACGCTGCAA CTGTTTTACA AAGCAATTTC 
AGAGGACACC GGGAGAGAAA GAGACTGAGA GCTGAGAAAG AAGAGGCAAC AGGAGGAGGG 
CAAAGCACTG GAGATGTGGA GGAGCAGGAG AACAATGACG TTCTAGACAT TACAGATGTT 
CAGCTGGAAC GAAGGGAAGA AGAGGAGGAA GGACAATACG ATGAGGAGCA GGCAGCCATT 
AAAATCCAGA GCAATTTTAG AGGCTATAAA GACCGAAAGA ACCTGAAAGC TACATCTCAA 
AAACAAGATG AGGAAGTGGA GTCACTCTTC AAACAGGTAT CCAAGGCCTC TGAAGACTAC 
CTTTCCCTTC AGAAGAAGTT AAATGAAATC ATCCTCGCCC ATCAGCTTAA CCCCAGCAAA 
AATGGCATGT TTGTGAAAGG ACAACCCATG AATGGCTACA TGGGCTACTA CCAGCCACCA 
GCTGACACAA AGCAGTCTCG AACACCACGC CGCGCACAAC AACCCAAGAC CCTGAACACG 
CCGGAGGACT CCACATACTA CACACTTATT CATAGGTCTG TCCAGGACGA TAAAAGGAGG 
CCGCGGAAAC AAAGCCCAAA AAAGTTGGAC AACGATGACC AATACTACCA GGGTTTGTCC 
AGCAGTAAAT CCACCAGCCT TCCCAATGAA CGACGCCGGT CGAGCAAGCG GAGGGCTTCT 
GATGAAAGAC GCTCCTCTGA AAGACCACTC TCTGATGTCC CCAAATCTGA AATCCCCAAA 
GACCCATTGG AGAAGAGGCA CTCGTTCTCT AAAATGCCCT CTACAGAGAG TCAAAGCGAG 
GACAACCCCT ACGACTACAG GAAGATACTG AGAAAGACCT CCCAGAGACG ACGACTAATA 
ACACAGCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic control of cellular morphogenesis in Müller glia.
Glia67(7)1401-1411(2019 07)
Charlton-Perkins M,Almeida AD,MacDonald RB,Harris WA

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Rbfox-regulated alternative splicing is critical for zebrafish cardiac and skeletal muscle functions.
Developmental biology359(2)251-61(2011 Nov)
Gallagher TL,Arribere JA,Geurts PA,Exner CR,McDonald KL,Dill KK,Marr HL,Adkar SS,Garnett AT,Amacher SL,Conboy JG

Identification and localization of myosin superfamily members in fish retina and retinal pigmented epithelium.
The Journal of comparative neurology513(2)209-23(2009 Mar)
Lin-Jones J,Sohlberg L,Dosé A,Breckler J,Hillman DW,Burnside B

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myo3a cDNA ORF clone, Danio rerio(zebrafish)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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